Investigation of ameliorative effects of indole-3 carbinol on TNBS-induced ulcerative colitis in rats.

IF 1.4 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnic & Histochemistry Pub Date : 2025-10-03 DOI:10.1080/10520295.2025.2561682

Erkmen Tuğrul Epikmen, Emrah İpek, Mehmet Hesapçıoğlu, Mehmet Karaboğa, Ali Riza Öztürk, Hamdi Avci

{"title":"Investigation of ameliorative effects of indole-3 carbinol on TNBS-induced ulcerative colitis in rats.","authors":"Erkmen Tuğrul Epikmen, Emrah İpek, Mehmet Hesapçıoğlu, Mehmet Karaboğa, Ali Riza Öztürk, Hamdi Avci","doi":"10.1080/10520295.2025.2561682","DOIUrl":null,"url":null,"abstract":"<p><p>Ulcerative colitis is a chronic inflammatory condition of the gastrointestinal tract that can predispose patients to colonic neoplasms. Various natural compounds have been explored for their therapeutic potential. Indole-3-carbinol (I3C), a natural compound derived from cruciferous vegetables, is recognized for its tissue-protective and regenerative properties. This study aimed to investigate the effects of I3C on experimental ulcerative colitis in rats. Thirty-two Wistar rats were randomly assigned to four groups: a control group receiving isotonic saline, a TNBS group administered trinitrobenzene sulfonic acid (TNBS) intrarectally, an I3C group receiving I3C via gastric gavage, and a TNBS+I3C group treated with I3C following TNBS induction. After 7 days, all animals were euthanized under anesthesia, and pathological, histochemical, and immunohistochemical evaluations were conducted. The results revealed that I3C mitigated the severity of TNBS-induced colonic lesions and facilitated tissue repair. The I3C-treated group exhibited reduced tissue damage and enhanced mucosal regeneration. Additionally, vessel count, collagen, and myofibroblastic activity were markedly increased following I3C treatment. In conclusion, I3C exhibits both protective and reparative effects in experimental ulcerative colitis, potentially through anti-inflammatory mechanisms and the activation of tissue repair pathways.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-11"},"PeriodicalIF":1.4000,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnic & Histochemistry","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1080/10520295.2025.2561682","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Ulcerative colitis is a chronic inflammatory condition of the gastrointestinal tract that can predispose patients to colonic neoplasms. Various natural compounds have been explored for their therapeutic potential. Indole-3-carbinol (I3C), a natural compound derived from cruciferous vegetables, is recognized for its tissue-protective and regenerative properties. This study aimed to investigate the effects of I3C on experimental ulcerative colitis in rats. Thirty-two Wistar rats were randomly assigned to four groups: a control group receiving isotonic saline, a TNBS group administered trinitrobenzene sulfonic acid (TNBS) intrarectally, an I3C group receiving I3C via gastric gavage, and a TNBS+I3C group treated with I3C following TNBS induction. After 7 days, all animals were euthanized under anesthesia, and pathological, histochemical, and immunohistochemical evaluations were conducted. The results revealed that I3C mitigated the severity of TNBS-induced colonic lesions and facilitated tissue repair. The I3C-treated group exhibited reduced tissue damage and enhanced mucosal regeneration. Additionally, vessel count, collagen, and myofibroblastic activity were markedly increased following I3C treatment. In conclusion, I3C exhibits both protective and reparative effects in experimental ulcerative colitis, potentially through anti-inflammatory mechanisms and the activation of tissue repair pathways.

查看原文本刊更多论文

吲哚-3甲醇对tnbs诱导的大鼠溃疡性结肠炎的改善作用研究。

溃疡性结肠炎是一种胃肠道慢性炎症性疾病，可使患者易患结肠肿瘤。各种天然化合物的治疗潜力已被探索。吲哚-3-甲醇（I3C）是一种从十字花科蔬菜中提取的天然化合物，具有组织保护和再生特性。本研究旨在探讨I3C对实验性溃疡性结肠炎大鼠的影响。32只Wistar大鼠随机分为4组：对照组给予等渗生理盐水，TNBS组给予三硝基苯磺酸（TNBS）， I3C组灌胃给予I3C， TNBS诱导后给予I3C治疗的TNBS+I3C组。7 d后麻醉安乐死，进行病理、组织化学和免疫组织化学评价。结果显示，I3C减轻了tnbs诱导的结肠病变的严重程度，促进了组织修复。i3c处理组组织损伤减少，粘膜再生增强。此外，血管计数、胶原蛋白和肌成纤维细胞活性在I3C治疗后显著增加。综上所述，I3C在实验性溃疡性结肠炎中具有保护和修复作用，可能通过抗炎机制和激活组织修复途径实现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biotechnic & Histochemistry 生物-生物工程与应用微生物

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.