Biotechnic & Histochemistry最新文献_第7页

Inula viscosa ameliorates acetic acid induced ulcerative colitis in rats. 粘胶菊对醋酸致大鼠溃疡性结肠炎的改善作用。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-05-01 DOI: 10.1080/10520295.2023.2176923

Mustafa Cellat, İbrahim Ozan Tekeli, Erdinç Türk, Tuba Aydin, Ahmet Uyar, Cafer Tayer İşler, İshak Gökçek, Muhammed Etyemez, Mehmet Güvenç

{"title":"Inula viscosa ameliorates acetic acid induced ulcerative colitis in rats.","authors":"Mustafa Cellat, İbrahim Ozan Tekeli, Erdinç Türk, Tuba Aydin, Ahmet Uyar, Cafer Tayer İşler, İshak Gökçek, Muhammed Etyemez, Mehmet Güvenç","doi":"10.1080/10520295.2023.2176923","DOIUrl":"https://doi.org/10.1080/10520295.2023.2176923","url":null,"abstract":"Increased pro-inflammatory cytokines and oxidative stress contribute to the pathophysiology of ulcerative colitis (UC). Inula viscosa is a plant with antioxidant and anti-inflammatory properties. We investigated the effect of an ethanolic extract of I. viscosa on an experimental UC model created using acetic acid. Rats were divided into four groups of eight: group 1, control; group 2, 3% acetic acid group; group 3, 100 mg/kg sulfasalazine + 3% acetic acid group; group 4, 400 mg/kg I. viscosa + 3% acetic acid. I. viscosa and sulfasalazine were administered by oral gavage and 3% acetic acid was administered per rectum. We found that I. viscosa treatment decreased colon malondialdehyde, tumor necrosis factor-α, interleukin-1 beta and nuclear factor kappa B levels; it increased reduced glutathione, nuclear factor erythroid 2-related factor 2, heme oxygenase-1 and kelch-like ECH-associated protein 1 levels and glutathione peroxidase enzyme activity. Group 1 colon exhibited normal histological structure. Slight inflammatory cell infiltration and edema and insignificant slight erosion in crypts were detected in colon tissues of group 4. We found that I. viscosa reduced oxidative stress and inflammation, which was protective against UC by inducing the Nrf-2/Keap-1/HO-1 pathway in the colon.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9446637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin ameliorates acute acrylamide induced spleen injury. 槲皮素改善急性丙烯酰胺性脾损伤。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-05-01 DOI: 10.1080/10520295.2023.2172610

Piyush Shukla, Naresh Kumar Sahu, Raj Kumar, Deep Kaur Dhalla, Samrat Rakshit, Monika Bhadauria, Narottam Das Agrawal, Sadhana Shrivastava, Sangeeta Shukla, Satendra Kumar Nirala

{"title":"Quercetin ameliorates acute acrylamide induced spleen injury.","authors":"Piyush Shukla, Naresh Kumar Sahu, Raj Kumar, Deep Kaur Dhalla, Samrat Rakshit, Monika Bhadauria, Narottam Das Agrawal, Sadhana Shrivastava, Sangeeta Shukla, Satendra Kumar Nirala","doi":"10.1080/10520295.2023.2172610","DOIUrl":"https://doi.org/10.1080/10520295.2023.2172610","url":null,"abstract":"Acrylamide is used for industrial and laboratory purposes; it also is produced during cooking of carbohydrate-rich food at high temperature. We investigated the therapeutic potential of quercetin for treatment of acute acrylamide induced injury to the spleen. We used female albino rats treated with acrylamide for 10 days followed by oral administration of quercetin in three doses for 5 days. We observed significantly reduced total body weight, spleen weight, red blood cells, total proteins, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glucose-6-phophate dehydrogenase, reduced glutathione, concentration of serum IgG and IgM after acrylamide induced toxicity compared to controls. We also found that white blood cells, triglycerides, cholesterol and lipid oxidation were increased significantly after acrylamide induced toxicity in rats compared to controls. Histoarchitecture of spleen was affected adversely by acrylamide toxicity. Administration of quercetin ameliorated adverse effects of acrylamide in a dose-dependent manner. Quercetin appears to ameliorate acrylamide induced injury to the spleen by increasing endogenous antioxidants and improving histoarchitecture and immune function.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9642001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Betatrophin, elabela, asprosin, glucagon and subfatin peptides in breast tissue, blood and milk in gestational diabetes. 妊娠糖尿病患者乳腺组织、血液和乳汁中的Betatrophin、elabela、asprosin、胰高血糖素和亚脂肪素肽。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-05-01 DOI: 10.1080/10520295.2023.2176546

Adem Yavuz, Mustafa Ata Aydin, Kader Ugur, Suna Aydin, Arzu Senol, Yakup Baykus, Rulin Deniz, İbrahim Sahin, Mehmet Hanifi Yalcin, Berrin Tarakci Gencer, Yaprak Kandemir Deniz, Sefer Ustebay, Zuhal Karaca Karagoz, Elif Emre, Suleyman Aydin

{"title":"Betatrophin, elabela, asprosin, glucagon and subfatin peptides in breast tissue, blood and milk in gestational diabetes.","authors":"Adem Yavuz, Mustafa Ata Aydin, Kader Ugur, Suna Aydin, Arzu Senol, Yakup Baykus, Rulin Deniz, İbrahim Sahin, Mehmet Hanifi Yalcin, Berrin Tarakci Gencer, Yaprak Kandemir Deniz, Sefer Ustebay, Zuhal Karaca Karagoz, Elif Emre, Suleyman Aydin","doi":"10.1080/10520295.2023.2176546","DOIUrl":"https://doi.org/10.1080/10520295.2023.2176546","url":null,"abstract":"We investigated the presence of asprosin (ASP), betatrophin, elabela (ELA), glucagon and subfatin (SUB) in the milk of mothers with gestational diabetes mellitus (GDM) and compared their levels with blood levels. We also investigated whether these peptides are synthesized by the breast. We investigated 12 volunteer mothers with GDM and 14 pregnant non-GDM control mothers. The peptides were measured using ELISA and their tissue localization was determined using immunohistochemistry. Breast milk contains ASP, betatrophin, ELA, glucagon and SUB. The amount of the peptides ranged from highest to the lowest in colostrum, transitional milk and mature milk. The amount of peptides in the milk was greater than for blood. The peptides, except for ELA, were increased in milk and blood by GDM. Betatrophin and ELA are synthesized in the connective tissue of the breast. ASP, glucagon and SUB are synthesized in the alveolar tissue of the breast. These peptides in breast milk may contribute to the development of the gastrointestinal tract of newborns and infants.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10057698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A tribute to Charles (Chuck) Willis. 致敬查尔斯(查克)威利斯。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-05-01 DOI: 10.1080/10520295.2023.2205206

David Penney

引用次数: 0

Seed coating with micronutrients improves germination, growth, yield and microelement nutrients of maize (Zea mays L.). 微量元素包种对玉米的萌发、生长、产量和微量元素养分均有促进作用。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-05-01 DOI: 10.1080/10520295.2023.2174273

Fa-Bo Chen, Yun-Chao Feng, Shi-Ping Huo

{"title":"Seed coating with micronutrients improves germination, growth, yield and microelement nutrients of maize (Zea mays L.).","authors":"Fa-Bo Chen, Yun-Chao Feng, Shi-Ping Huo","doi":"10.1080/10520295.2023.2174273","DOIUrl":"https://doi.org/10.1080/10520295.2023.2174273","url":null,"abstract":"Soil and foliar application are the most widely used methods for adding micronutrients to maize. High quality micronutrient fertilizers, however, are difficult to obtain in developing countries; micronutrient seed coatings are an attractive and practical alternative. We applied this approach to maize (Zea mays L.) to demonstrate the effects of boron (B), iron (Fe), manganese (Mn), molybdenum (Mo) and zinc (Zn) sulfates on maize germination, vigor, seedling growth, seed yield and seed quality as well as on seed microelement concentration. Seed coating was tested on three representative Chinese soil types (sandy, purple and lime soils). Compared to untreated controls, coating maize seeds with micronutrients significantly increased the seed emergence rate, seedling height, leaf length, leaf width, leaf area, main root length, root number, above ground fresh biomass, above ground dry biomass, underground fresh biomass, underground dry biomass, ear thickness and yield in sandy, purple and lime soils. Coating maize seeds with micronutrients also significantly increased the yield and quality of maize seed compared to untreated controls including ear barren tip, ear length, ear thickness, grains/row, hundred seed weigh, and rows/ear. Also, B, Zn, Fe, Mn and Mo microelements accumulated in maize seed after coating the seed with micronutrients. Our findings indicate that micronutrient seed coating may improve nutrient uptake and production of maize hybrids.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9446639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A fluorescence method to detect cyanophycin in the symbiotic cyanobacterium, Anabaena azollae. 荧光法检测共生蓝细菌中紫藻素的含量。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-05-01 DOI: 10.1080/10520295.2023.2182452

Ana L Pereira, Francisco Carrapiço

引用次数: 0

Comparison of proliferation and osteogenic differentiation potential of bovine adipose tissue and bone marrow derived stem cells. 牛脂肪组织和骨髓干细胞的增殖和成骨分化潜能比较。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-05-01 DOI: 10.1080/10520295.2023.2177347

Ozlem Ozden Akkaya, Tayfun Dikmen, Shah Nawaz, Asm Golam Kibria, Korhan Altunbaş, Artay Yağci, Metin Erdoğan, Mustafa Volkan Yaprakci

{"title":"Comparison of proliferation and osteogenic differentiation potential of bovine adipose tissue and bone marrow derived stem cells.","authors":"Ozlem Ozden Akkaya, Tayfun Dikmen, Shah Nawaz, Asm Golam Kibria, Korhan Altunbaş, Artay Yağci, Metin Erdoğan, Mustafa Volkan Yaprakci","doi":"10.1080/10520295.2023.2177347","DOIUrl":"https://doi.org/10.1080/10520295.2023.2177347","url":null,"abstract":"Bone marrow derived stem cells (BMSC) are the most utilized cell type in the field of bone regeneration. Although BMSC are both safe and efficacious, the search for alternative sources for stem cells continues. We investigated bovine BMSC and adipose tissue derived mesenchymal stem cells (ATSC) using immunofluorescence and PCR. We further compared the osteogenic differentiation potentials of both sources of stem cells. We assessed alkaline phosphatase (ALP) enzyme levels and calcium deposition in differentiating cells at days 7, 14 and 21 to compare the osteogenic differentiation capability of both cell types. We found that ATSC expressed significantly higher ALP levels compared to BMSC throughout osteogenic differentiation. Calcium deposition was greater in ATSC than BMSC at days 7 and 14. By the end of day 21, BMSC produced greater calcium deposition. We found that ATSC undergo osteogenic differentiation more rapidly than BMSC, but BMSC provide greater mineralization over longer periods.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9499855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying stem cells in the main excretory ducts of rat major salivary glands: adventures with commercial antibodies. 鉴定大鼠大唾液腺主要排泄管中的干细胞:商业抗体的冒险。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-05-01 DOI: 10.1080/10520295.2023.2177348

Robert S Redman, José C Alvarez-Martinez

{"title":"Identifying stem cells in the main excretory ducts of rat major salivary glands: adventures with commercial antibodies.","authors":"Robert S Redman, José C Alvarez-Martinez","doi":"10.1080/10520295.2023.2177348","DOIUrl":"https://doi.org/10.1080/10520295.2023.2177348","url":null,"abstract":"We investigated the entire length of the main excretory ducts (MED) of the major sublingual, parotid and submandibular salivary glands of mature laboratory rats for mucous (goblet) and luminal ciliated cells, biomarkers of cell proliferation, apoptosis, and five biomarkers of stem cells. Spleen and testis were used as positive controls. We used formalin fixed, paraffin embedded tissues. No mucous cells or cells with luminal cilia were observed in hematoxylin and eosin, alcian blue or periodic acid-Schiff stained sections. Immunohistochemistry using rabbit anti-rat antibodies produced anomalous reactions with cleaved caspase-3 for apoptosis, Ki-67 for proliferative activity and Sox 2. Following antigen retrieval, no primary antibody and all three negative controls, labeled macrophages appeared in the spleen. TUNEL staining revealed a few cells per section undergoing apoptosis. Reactions deemed valid occurred in MED with cytokeratin-5 and c-Kit and stem cell antigen 1 (Sca-1) mostly in the gland and middle segments. Other ducts, but not acini or myoepithelial cells, also were variably stained with c-Kit and Sca-1.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9666196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of silibinin on apoptosis and insulin secretion in rat RINm5F pancreatic β-cells. 水飞蓟宾对大鼠RINm5F胰腺β-细胞凋亡和胰岛素分泌的影响。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2022.2154840

Maryam Mazraesefidi, Mehdi Mahmoodi, Mohammadreza Hajizadeh

{"title":"Effects of silibinin on apoptosis and insulin secretion in rat RINm5F pancreatic β-cells.","authors":"Maryam Mazraesefidi, Mehdi Mahmoodi, Mohammadreza Hajizadeh","doi":"10.1080/10520295.2022.2154840","DOIUrl":"https://doi.org/10.1080/10520295.2022.2154840","url":null,"abstract":"We investigated whether silibinin, a flavonoid, might be useful for treating diabetes mellitus by treating five groups of rat RINm5F β-insulinemia cells as follows: control streptozotocin (STZ) group administered citrate buffer and dimethyl sulfoxide; STZ group administered 20 mM STZ; silibinin group administered 50 µM silibinin; pre-silibinin group administered 50 µM silibinin 5 h before administering 20 mM STZ; simultaneous group administered 50 µM silibinin at the same time as 20 mM STZ. For all groups, MTT assay and flow cytometry were used to evaluate cell viability and necrosis, respectively. Glucose-stimulated insulin secretion (GSIS) and insulin cell content were determined using enzyme-linked immunosorbent assay. Also, expression of genes, pancreatic and duodenal homeobox 1 (pdx1), neuronal differentiation 1 (neurod1), v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A (mafa), glucose transporter 2 (glut2)) was determined using the real-time polymerase chain reaction. We found that silibinin improved the viability of RINm5F cells and increased GSIS and cellular insulin under glucotoxic conditions. Silibinin increased the expression of neurod1, mafa and glut2, but reduced pdx1 expression. Our findings suggest that silibinin might increase glucose sensitivity and insulin synthesis under glucotoxic conditions, which could be useful for diabetes treatment.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9449041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of apocynin on sciatic nerve injury in rabbits. 罗布麻素对兔坐骨神经损伤的影响。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-04-01 Epub Date: 2022-11-28 DOI: 10.1080/10520295.2022.2146195

Mehmet Akif Durak, Onural Ozhan, Bora Tetik, Azibe Yildiz, Zeynep Aksungur, Nigar Vardi, Yusuf Turkoz, Muharrem Ucar, Hakan Parlakpinar

引用次数: 1