Biotechnic & Histochemistry最新文献_第8页

Effects of silibinin on apoptosis and insulin secretion in rat RINm5F pancreatic β-cells. 水飞蓟宾对大鼠RINm5F胰腺β-细胞凋亡和胰岛素分泌的影响。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2022.2154840

Maryam Mazraesefidi, Mehdi Mahmoodi, Mohammadreza Hajizadeh

{"title":"Effects of silibinin on apoptosis and insulin secretion in rat RINm5F pancreatic β-cells.","authors":"Maryam Mazraesefidi, Mehdi Mahmoodi, Mohammadreza Hajizadeh","doi":"10.1080/10520295.2022.2154840","DOIUrl":"https://doi.org/10.1080/10520295.2022.2154840","url":null,"abstract":"We investigated whether silibinin, a flavonoid, might be useful for treating diabetes mellitus by treating five groups of rat RINm5F β-insulinemia cells as follows: control streptozotocin (STZ) group administered citrate buffer and dimethyl sulfoxide; STZ group administered 20 mM STZ; silibinin group administered 50 µM silibinin; pre-silibinin group administered 50 µM silibinin 5 h before administering 20 mM STZ; simultaneous group administered 50 µM silibinin at the same time as 20 mM STZ. For all groups, MTT assay and flow cytometry were used to evaluate cell viability and necrosis, respectively. Glucose-stimulated insulin secretion (GSIS) and insulin cell content were determined using enzyme-linked immunosorbent assay. Also, expression of genes, pancreatic and duodenal homeobox 1 (pdx1), neuronal differentiation 1 (neurod1), v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A (mafa), glucose transporter 2 (glut2)) was determined using the real-time polymerase chain reaction. We found that silibinin improved the viability of RINm5F cells and increased GSIS and cellular insulin under glucotoxic conditions. Silibinin increased the expression of neurod1, mafa and glut2, but reduced pdx1 expression. Our findings suggest that silibinin might increase glucose sensitivity and insulin synthesis under glucotoxic conditions, which could be useful for diabetes treatment.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":"98 3","pages":"201-209"},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9449041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of apocynin on sciatic nerve injury in rabbits. 罗布麻素对兔坐骨神经损伤的影响。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-04-01 Epub Date: 2022-11-28 DOI: 10.1080/10520295.2022.2146195

Mehmet Akif Durak, Onural Ozhan, Bora Tetik, Azibe Yildiz, Zeynep Aksungur, Nigar Vardi, Yusuf Turkoz, Muharrem Ucar, Hakan Parlakpinar

引用次数: 1

Expression of IRS2 in the female reproductive system during the estrous cycle in mice. 小鼠发情周期中雌性生殖系统中IRS2的表达。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2022.2153167

Zhongli Wang, Benyi Yi, Lijun Gan, Xiuli Li, Xiuxiu Liu, Qizhuang Lv, Lei Yang

引用次数: 0

Effects of agomelatine on rat liver regeneration following partial hepatectomy. 阿戈美拉汀对大鼠肝部分切除后肝脏再生的影响。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2022.2139862

A Kose, O Ozhan, H Parlakpinar, N Vardi, A Yildiz, Y Turkoz, Z Erdemli, Y Bilgic, M E Sarihan

{"title":"Effects of agomelatine on rat liver regeneration following partial hepatectomy.","authors":"A Kose, O Ozhan, H Parlakpinar, N Vardi, A Yildiz, Y Turkoz, Z Erdemli, Y Bilgic, M E Sarihan","doi":"10.1080/10520295.2022.2139862","DOIUrl":"https://doi.org/10.1080/10520295.2022.2139862","url":null,"abstract":"Primary or metastatic hepatic malignancies are common. Partial hepatectomy (PH) is the primary treatment for both benign and malignant hepatic neoplasms; it also is used for living donor liver transplantation. The regenerative potential of the liver after PH is 70-80% in humans. We investigated the protective and therapeutic effects of agomelatine (AGM) on rat liver regeneration following PH. We used 32 rats distributed equally into four groups: group 1, sham control; group 2, PH group; group 3, administered 20 mg/kg AGM orally once/day for 7 days following PH; group 4, administered 20 mg/kg AGM orally once/day 3 days before and 7 days following PH for 10 days. Liver samples were analyzed for antioxidants and free radicals. Tissue samples were processed and stained with hematoxylin and eosin to assess histopathological status and stained immunohistochemically for Ki-67. We found that PH reduced antioxidant enzymes and increased tissue reactive oxygen species, whereas AGM treatment had the opposite effect on these parameters. Our biochemical and histopathological findings were consistent. PH caused sinusoid congestion and dilation. Intensity of Ki-67 immunostaining of hepatocytes was increased in group 2, whereas these were reduced in group 4. Intensity of Ki-67 immunostaining of hepatocytes was increased in group 2, whereas it was reduced in the group 4 compared to group 1. We found that AGM was hepatoprotective following PH due to its antioxidant and free radical scavenger properties.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":"98 3","pages":"157-165"},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9094553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Olanzapine treatment of lactating females causes testicular atrophy in prepuberal rat offspring. 奥氮平治疗哺乳期雌性引起青春期前大鼠后代睾丸萎缩。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2022.2150314

Luiz André Rodrigues Lima, Sandra Maria Torres, Simone Regina Barros Macêdo, Fernanda das Chagas Angelo Mendes Tenorio, Bruno Mendes Tenorio, Valdemiro Amaro da Silva Junior

{"title":"Olanzapine treatment of lactating females causes testicular atrophy in prepuberal rat offspring.","authors":"Luiz André Rodrigues Lima, Sandra Maria Torres, Simone Regina Barros Macêdo, Fernanda das Chagas Angelo Mendes Tenorio, Bruno Mendes Tenorio, Valdemiro Amaro da Silva Junior","doi":"10.1080/10520295.2022.2150314","DOIUrl":"https://doi.org/10.1080/10520295.2022.2150314","url":null,"abstract":"The antipsychotic drug, olanzapine, is prescribed for postpartum psychosis. Possible adverse effects on fertility of offspring are unclear. We investigated the effects of administering olanzapine via lactation on testicular development and endocrine function of prepuberal male rats. Olanzapine was administered to mothers at 2.5, 5 or 10 mg/kg. We found in male offspring increased body weight, decreased gonadosomatic index, testicular weight and epididymal weight. The volume of seminiferous tubules, seminiferous epithelium, Leydig cells, intertubule tissue and lymphatic space was reduced in rat pups exposed to olanzapine. Tubule diameter and length, seminiferous epithelium height, Leydig cell size and nuclear diameter also were reduced. Testosterone levels were reduced in the groups exposed to olanzapine, while prolactin levels were increased. We observed histopathology in testes of animals whose mothers had been treated with 2.5 mg/kg olanzapine; more severe pathology was observed in offspring whose mothers were administered higher doses. Administration of olanzapine to mothers during lactation produced testicular and endocrine pathology in prepuberal rats in a dose-dependent manner.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":"98 3","pages":"179-186"},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9149105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Protective effect of adenosine triphosphate and benidipine separately or together against cardiotoxicity caused by bevacizumab. 三磷酸腺苷和苯地平单独或联合对贝伐单抗引起的心脏毒性的保护作用。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2022.2153385

Erkan Yıldırım, Nilgun Yıldırım, Mahir Cengiz, Gulce Naz Yazıcı, Resit Coskun, Bahadır Suleyman, Abdulkadir Coban, Halis Suleyman

{"title":"Protective effect of adenosine triphosphate and benidipine separately or together against cardiotoxicity caused by bevacizumab.","authors":"Erkan Yıldırım, Nilgun Yıldırım, Mahir Cengiz, Gulce Naz Yazıcı, Resit Coskun, Bahadır Suleyman, Abdulkadir Coban, Halis Suleyman","doi":"10.1080/10520295.2022.2153385","DOIUrl":"https://doi.org/10.1080/10520295.2022.2153385","url":null,"abstract":"Bevacizumab is a recombinant humanized monoclonal antibody whose adverse effects include cardiotoxicity. We investigated whether using adenosine triphosphate (ATP) or benidipine either separately or together protects against cardiac damage induced by bevacizumab in rats. Forty Wistar albino male rats were allocated to five groups of eight: bevacizumab (Bv), ATP + bevacizumab (ABv), benidipine + bevacizumab (BBv), ATP + benidipine + bevacizumab (ABBv) and untreated controls. Rats in the ABv group were injected intraperitoneally (i.p.) with 2 mg/kg ATP. The BBv group was given 4 mg/kg benidipine by oral gavage. The ABBv group was injected i.p. with 2 mg/kg ATP and simultaneously administered 4 mg/kg benidipine orally. One hour after administration of ATP, benidipine or normal saline, the Bv, ABv, BBv and ABBv groups were injected i.p. with 10 mg/kg bevacizumab. Malondialdehyde (MDA) and total glutathione (tGSH) levels were measured in cardiac tissue, and troponin I (TP I) and creatine kinase MB (CK-MB) levels were measured in blood samples. Tissue samples were examined for histopathology. We found the lowest TP I, CK-MB and MDA levels and the highest tGSH level in the ABBv group; these results were similar to the control group. Nuclei of cardiomyocytes in the BV group were misshapen and shrunken, and myofibers were disrupted; we also observed eosinophilic degeneration and interstitial edema. Blood capillaries were dilated and congested. We observed amelioration of these findings in the ABBv group. We found that ATP and benidipine alone or in combination reduced cardiac damage associated with the use of bevacizumab. ATP + benidipine combined therapy produced the most favorable results.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":"98 3","pages":"193-200"},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9463329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visualization of the carotid body in situ in fixed human carotid bifurcations using a xylene-based tissue clearing method. 使用二甲苯为基础的组织清除方法原位观察固定人颈动脉分叉的颈动脉体。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2022.2140831

Daphne Verdoorn, Cindy Gj Cleypool, Claire Mackaaij, Ronald Law Bleys

引用次数: 0

Effects of diazoxide on streptozotocin induced β cell damage via HSP70/HSP90/TLR4/AMPK signaling pathways. 二氮氧化物通过HSP70/HSP90/TLR4/AMPK信号通路对链脲佐菌素诱导的β细胞损伤的影响

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2023.2168757

Salih Tunc Kaya

{"title":"Effects of diazoxide on streptozotocin induced β cell damage via HSP70/HSP90/TLR4/AMPK signaling pathways.","authors":"Salih Tunc Kaya","doi":"10.1080/10520295.2023.2168757","DOIUrl":"https://doi.org/10.1080/10520295.2023.2168757","url":null,"abstract":"I investigated the effects of diazoxide, a mitochondrial potassium channel opener, on streptozotocin (STZ) induced pancreatic β cell damage via the HSP70/HSP90/TLR4/AMPK signaling pathways in vitro. I used the pancreatic β cell line, 1.1B4, to create four groups: control, STZ treated, diazoxide treated, STZ + diazoxide treated. The STZ treated cells were exposed to 20 µM STZ for 2 h with or without 100 µM diazoxide for 24 h. Total antioxidant status (TAS), total oxidant status (TOS), cell viability and mitochondrial membrane potential (MMP) were measured. Expression of ATP-sensitive potassium channel (KATP) subunits, heat shock protein-70 (HSP70), heat shock protein-90 (HSP90), toll-like receptor 4 (TLR4), AMP-activated protein kinase (AMPK) and some apoptotic proteins were detected using western blotting. Apoptosis was assessed using TUNEL staining. STZ increased TOS and OSI in the pancreatic β cells; however, diazoxide failed to improve oxidative stress. Also, STZ increased tunnel positive cells in the pancreatic β cells. Diazoxide decreased the tunnel positive cells in the STZ treated β cell. STZ decreased MMP; however, diazoxide did not normalize MMP in the STZ induced β cells. Diazoxide increased the HSP70:HSP90 protein expression ratio. STZ decreased expression of AMPK and subunits of KATP channel and increased the expression of caspase-3 and TLR4 protein; diazoxide normalized the expression of all proteins studied. KATP channel opening by diazoxide protects pancreatic β cells against STZ toxicity via HSP70/HSP90/TLR4/AMPK signaling.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":"98 3","pages":"210-219"},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9151093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stains recently certified. 最近认证的污渍。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-03-01 DOI: 10.1080/10520295.2023.2184029

引用次数: 0

Stains recently certified. 最近认证的污渍。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-02-08 DOI: 10.1080/10520295.2023.2174670

引用次数: 0