Biotechnic & Histochemistry最新文献

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Expression of IRS2 in the female reproductive system during the estrous cycle in mice. 小鼠发情周期中雌性生殖系统中IRS2的表达。
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2022.2153167
Zhongli Wang, Benyi Yi, Lijun Gan, Xiuli Li, Xiuxiu Liu, Qizhuang Lv, Lei Yang
{"title":"Expression of IRS2 in the female reproductive system during the estrous cycle in mice.","authors":"Zhongli Wang,&nbsp;Benyi Yi,&nbsp;Lijun Gan,&nbsp;Xiuli Li,&nbsp;Xiuxiu Liu,&nbsp;Qizhuang Lv,&nbsp;Lei Yang","doi":"10.1080/10520295.2022.2153167","DOIUrl":"https://doi.org/10.1080/10520295.2022.2153167","url":null,"abstract":"<p><p>Insulin receptor substrate 2 (IRS2) participates in reproduction; however, the location and expression of IRS2 in the reproductive system of female mice is not clear. We used real-time quantitative polymerase chain reaction (RT-PCR), western blot and immunohistochemical staining to investigate the expression of IRS2 in the ovary, oviduct and uterus of female mice during the estrous cycle. We found that IRS2 was expressed in all reproductive organs of mouse and that the expression level changed with the estrous phases. The expression of IRS2 in reproductive organs was greatest during estrus.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9621396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of agomelatine on rat liver regeneration following partial hepatectomy. 阿戈美拉汀对大鼠肝部分切除后肝脏再生的影响。
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2022.2139862
A Kose, O Ozhan, H Parlakpinar, N Vardi, A Yildiz, Y Turkoz, Z Erdemli, Y Bilgic, M E Sarihan
{"title":"Effects of agomelatine on rat liver regeneration following partial hepatectomy.","authors":"A Kose,&nbsp;O Ozhan,&nbsp;H Parlakpinar,&nbsp;N Vardi,&nbsp;A Yildiz,&nbsp;Y Turkoz,&nbsp;Z Erdemli,&nbsp;Y Bilgic,&nbsp;M E Sarihan","doi":"10.1080/10520295.2022.2139862","DOIUrl":"https://doi.org/10.1080/10520295.2022.2139862","url":null,"abstract":"<p><p>Primary or metastatic hepatic malignancies are common. Partial hepatectomy (PH) is the primary treatment for both benign and malignant hepatic neoplasms; it also is used for living donor liver transplantation. The regenerative potential of the liver after PH is 70-80% in humans. We investigated the protective and therapeutic effects of agomelatine (AGM) on rat liver regeneration following PH. We used 32 rats distributed equally into four groups: group 1, sham control; group 2, PH group; group 3, administered 20 mg/kg AGM orally once/day for 7 days following PH; group 4, administered 20 mg/kg AGM orally once/day 3 days before and 7 days following PH for 10 days. Liver samples were analyzed for antioxidants and free radicals. Tissue samples were processed and stained with hematoxylin and eosin to assess histopathological status and stained immunohistochemically for Ki-67. We found that PH reduced antioxidant enzymes and increased tissue reactive oxygen species, whereas AGM treatment had the opposite effect on these parameters. Our biochemical and histopathological findings were consistent. PH caused sinusoid congestion and dilation. Intensity of Ki-67 immunostaining of hepatocytes was increased in group 2, whereas these were reduced in group 4. Intensity of Ki-67 immunostaining of hepatocytes was increased in group 2, whereas it was reduced in the group 4 compared to group 1. We found that AGM was hepatoprotective following PH due to its antioxidant and free radical scavenger properties.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9094553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Olanzapine treatment of lactating females causes testicular atrophy in prepuberal rat offspring. 奥氮平治疗哺乳期雌性引起青春期前大鼠后代睾丸萎缩。
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2022.2150314
Luiz André Rodrigues Lima, Sandra Maria Torres, Simone Regina Barros Macêdo, Fernanda das Chagas Angelo Mendes Tenorio, Bruno Mendes Tenorio, Valdemiro Amaro da Silva Junior
{"title":"Olanzapine treatment of lactating females causes testicular atrophy in prepuberal rat offspring.","authors":"Luiz André Rodrigues Lima,&nbsp;Sandra Maria Torres,&nbsp;Simone Regina Barros Macêdo,&nbsp;Fernanda das Chagas Angelo Mendes Tenorio,&nbsp;Bruno Mendes Tenorio,&nbsp;Valdemiro Amaro da Silva Junior","doi":"10.1080/10520295.2022.2150314","DOIUrl":"https://doi.org/10.1080/10520295.2022.2150314","url":null,"abstract":"<p><p>The antipsychotic drug, olanzapine, is prescribed for postpartum psychosis. Possible adverse effects on fertility of offspring are unclear. We investigated the effects of administering olanzapine via lactation on testicular development and endocrine function of prepuberal male rats. Olanzapine was administered to mothers at 2.5, 5 or 10 mg/kg. We found in male offspring increased body weight, decreased gonadosomatic index, testicular weight and epididymal weight. The volume of seminiferous tubules, seminiferous epithelium, Leydig cells, intertubule tissue and lymphatic space was reduced in rat pups exposed to olanzapine. Tubule diameter and length, seminiferous epithelium height, Leydig cell size and nuclear diameter also were reduced. Testosterone levels were reduced in the groups exposed to olanzapine, while prolactin levels were increased. We observed histopathology in testes of animals whose mothers had been treated with 2.5 mg/kg olanzapine; more severe pathology was observed in offspring whose mothers were administered higher doses. Administration of olanzapine to mothers during lactation produced testicular and endocrine pathology in prepuberal rats in a dose-dependent manner.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9149105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Protective effect of adenosine triphosphate and benidipine separately or together against cardiotoxicity caused by bevacizumab. 三磷酸腺苷和苯地平单独或联合对贝伐单抗引起的心脏毒性的保护作用。
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2022.2153385
Erkan Yıldırım, Nilgun Yıldırım, Mahir Cengiz, Gulce Naz Yazıcı, Resit Coskun, Bahadır Suleyman, Abdulkadir Coban, Halis Suleyman
{"title":"Protective effect of adenosine triphosphate and benidipine separately or together against cardiotoxicity caused by bevacizumab.","authors":"Erkan Yıldırım,&nbsp;Nilgun Yıldırım,&nbsp;Mahir Cengiz,&nbsp;Gulce Naz Yazıcı,&nbsp;Resit Coskun,&nbsp;Bahadır Suleyman,&nbsp;Abdulkadir Coban,&nbsp;Halis Suleyman","doi":"10.1080/10520295.2022.2153385","DOIUrl":"https://doi.org/10.1080/10520295.2022.2153385","url":null,"abstract":"<p><p>Bevacizumab is a recombinant humanized monoclonal antibody whose adverse effects include cardiotoxicity. We investigated whether using adenosine triphosphate (ATP) or benidipine either separately or together protects against cardiac damage induced by bevacizumab in rats. Forty Wistar albino male rats were allocated to five groups of eight: bevacizumab (Bv), ATP + bevacizumab (ABv), benidipine + bevacizumab (BBv), ATP + benidipine + bevacizumab (ABBv) and untreated controls. Rats in the ABv group were injected intraperitoneally (i.p.) with 2 mg/kg ATP. The BBv group was given 4 mg/kg benidipine by oral gavage. The ABBv group was injected i.p. with 2 mg/kg ATP and simultaneously administered 4 mg/kg benidipine orally. One hour after administration of ATP, benidipine or normal saline, the Bv, ABv, BBv and ABBv groups were injected i.p. with 10 mg/kg bevacizumab. Malondialdehyde (MDA) and total glutathione (tGSH) levels were measured in cardiac tissue, and troponin I (TP I) and creatine kinase MB (CK-MB) levels were measured in blood samples. Tissue samples were examined for histopathology. We found the lowest TP I, CK-MB and MDA levels and the highest tGSH level in the ABBv group; these results were similar to the control group. Nuclei of cardiomyocytes in the BV group were misshapen and shrunken, and myofibers were disrupted; we also observed eosinophilic degeneration and interstitial edema. Blood capillaries were dilated and congested. We observed amelioration of these findings in the ABBv group. We found that ATP and benidipine alone or in combination reduced cardiac damage associated with the use of bevacizumab. ATP + benidipine combined therapy produced the most favorable results.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9463329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Visualization of the carotid body in situ in fixed human carotid bifurcations using a xylene-based tissue clearing method. 使用二甲苯为基础的组织清除方法原位观察固定人颈动脉分叉的颈动脉体。
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2022.2140831
Daphne Verdoorn, Cindy Gj Cleypool, Claire Mackaaij, Ronald Law Bleys
{"title":"Visualization of the carotid body in situ in fixed human carotid bifurcations using a xylene-based tissue clearing method.","authors":"Daphne Verdoorn,&nbsp;Cindy Gj Cleypool,&nbsp;Claire Mackaaij,&nbsp;Ronald Law Bleys","doi":"10.1080/10520295.2022.2140831","DOIUrl":"https://doi.org/10.1080/10520295.2022.2140831","url":null,"abstract":"<p><p>The anatomy of the carotid body (CB) and its nerve supply are important, because it is a potential therapeutic target for treatment of various clinical conditions. Visualization of the CB in situ in fixed human anatomical specimens is hampered by obscuring adipose and connective tissues. We developed a tissue clearing method to optimize identification of the CB. We used single sided carotid bifurcations of six human cadavers fixed long term. Visualization of the CB was accomplished by clearing tissue with xylene. Under incident light, carotid bifurcations exhibited a less transparent, darker colored CB; hematoxylin and eosin stained paraffin sections confirmed its identity. Our visualization of the CB in situ in human carotid bifurcations fixed long term enabled targeted resection and subsequent topographic and morphometric measurements of the CB. Our procedure does not interfere with immunohistochemical staining of sections prepared from such specimens.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9096209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of diazoxide on streptozotocin induced β cell damage via HSP70/HSP90/TLR4/AMPK signaling pathways. 二氮氧化物通过HSP70/HSP90/TLR4/AMPK信号通路对链脲佐菌素诱导的β细胞损伤的影响
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2023.2168757
Salih Tunc Kaya
{"title":"Effects of diazoxide on streptozotocin induced β cell damage via HSP70/HSP90/TLR4/AMPK signaling pathways.","authors":"Salih Tunc Kaya","doi":"10.1080/10520295.2023.2168757","DOIUrl":"https://doi.org/10.1080/10520295.2023.2168757","url":null,"abstract":"<p><p>I investigated the effects of diazoxide, a mitochondrial potassium channel opener, on streptozotocin (STZ) induced pancreatic β cell damage via the HSP70/HSP90/TLR4/AMPK signaling pathways in vitro. I used the pancreatic β cell line, 1.1B4, to create four groups: control, STZ treated, diazoxide treated, STZ + diazoxide treated. The STZ treated cells were exposed to 20 µM STZ for 2 h with or without 100 µM diazoxide for 24 h. Total antioxidant status (TAS), total oxidant status (TOS), cell viability and mitochondrial membrane potential (MMP) were measured. Expression of ATP-sensitive potassium channel (K<sub>ATP</sub>) subunits, heat shock protein-70 (HSP70), heat shock protein-90 (HSP90), toll-like receptor 4 (TLR4), AMP-activated protein kinase (AMPK) and some apoptotic proteins were detected using western blotting. Apoptosis was assessed using TUNEL staining. STZ increased TOS and OSI in the pancreatic β cells; however, diazoxide failed to improve oxidative stress. Also, STZ increased tunnel positive cells in the pancreatic β cells. Diazoxide decreased the tunnel positive cells in the STZ treated β cell. STZ decreased MMP; however, diazoxide did not normalize MMP in the STZ induced β cells. Diazoxide increased the HSP70:HSP90 protein expression ratio. STZ decreased expression of AMPK and subunits of K<sub>ATP</sub> channel and increased the expression of caspase-3 and TLR4 protein; diazoxide normalized the expression of all proteins studied. K<sub>ATP</sub> channel opening by diazoxide protects pancreatic β cells against STZ toxicity via HSP70/HSP90/TLR4/AMPK signaling.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9151093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stains recently certified. 最近认证的污渍。
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2023-03-01 DOI: 10.1080/10520295.2023.2184029
{"title":"Stains recently certified.","authors":"","doi":"10.1080/10520295.2023.2184029","DOIUrl":"https://doi.org/10.1080/10520295.2023.2184029","url":null,"abstract":"","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10813994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stains recently certified. 最近认证的污渍。
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2023-02-08 DOI: 10.1080/10520295.2023.2174670
{"title":"Stains recently certified.","authors":"","doi":"10.1080/10520295.2023.2174670","DOIUrl":"https://doi.org/10.1080/10520295.2023.2174670","url":null,"abstract":"","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10668232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stains recently certified. 最近认证的污渍。
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2023-02-07 DOI: 10.1080/10520295.2023.2174669
{"title":"Stains recently certified.","authors":"","doi":"10.1080/10520295.2023.2174669","DOIUrl":"https://doi.org/10.1080/10520295.2023.2174669","url":null,"abstract":"","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10654175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stains recently certified. 最近认证的污渍。
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2023-02-07 DOI: 10.1080/10520295.2023.2174668
{"title":"Stains recently certified.","authors":"","doi":"10.1080/10520295.2023.2174668","DOIUrl":"https://doi.org/10.1080/10520295.2023.2174668","url":null,"abstract":"","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10654602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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