Serum perilipin-2 levels in a rat model of liver ischemia-reperfusion injury.

IF 1.4 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnic & Histochemistry Pub Date : 2025-08-01 Epub Date: 2025-07-18 DOI:10.1080/10520295.2025.2528036

Turan Can Yıldız, Kemal Eyvaz, Hamit Yaşar Ellidag, Ayşen Kılıçarslan, Ömer Çelik, Remzi Can Çakır, Erhan Aydemir, Arif Aslaner

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Abstract

Liver ischemia and ischemia/reperfusion (I/R) injury are common in hepatic resection, trauma surgery, and transplantation and contribute to postoperative morbidity and mortality. This study aimed to investigate the relationship between early histopathological changes due to liver I/R injury and serum levels of perilipin-2 (Plin2) and other oxidative stress biomarkers. Fifty female Wistar albino rats were divided into five groups: Group 1 (control), Group 2 (ischemia for 30 minutes), and Groups 3-5 (ischemia followed by 1, 2, or 3 hours of reperfusion). Intracardiac and arterial blood samples were collected for biochemical analysis, while liver tissue samples were used for histopathological examination. Serum levels of Plin2, ischemia-modified albumin (IMA), total antioxidant status (TAS), and total oxidant status (TOS) were measured. Plin2 levels were significantly lower in the ischemia group compared to others (p < 0.01). The control group had significantly lower IMA, TOS, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) levels (p < 0.01) and lower TAS levels (p < 0.05). Rats with Grade 1 liver injury showed significantly lower Plin2 (p < 0.01) and higher IMA levels (p < 0.05). Reduced serum Plin2 following ischemia suggests its potential as a biomarker for acute liver injury. Further studies are needed to clarify its role across different reperfusion durations.

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肝缺血再灌注损伤模型大鼠血清perilipin-2水平。

肝缺血和缺血/再灌注（I/R）损伤在肝切除术、创伤手术和肝移植中很常见，是术后发病率和死亡率的重要因素。本研究旨在探讨肝I/R损伤引起的早期组织病理学改变与血清periilipin -2 （Plin2）及其他氧化应激生物标志物水平的关系。将50只雌性Wistar白化大鼠分为5组：1组（对照组）、2组（缺血30min）和3-5组（缺血后再灌注1,2,3 h）。取心内、动脉血进行生化分析，取肝组织标本进行组织病理学检查。测定血清Plin2、缺血修饰白蛋白（IMA）、总抗氧化状态（TAS）和总氧化状态（TOS）水平。缺血组Plin2水平明显低于其他组(p p p p p p

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来源期刊

Biotechnic & Histochemistry 生物-生物工程与应用微生物

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.