Biomedical optics express最新文献_第4页

Wide-field and non-invasive imaging of brain tumours with scattered light techniques. 用散射光技术进行脑肿瘤的宽视场无创成像。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-02-02 eCollection Date: 2026-03-01 DOI: 10.1364/BOE.587407

Philip Binner, Jack Radford, Ilya Starshynov, Mansa Madhusudan, Karen Strathdee, Katrina Stevenson, Matthew Walker, Giuseppe Ciccone, Gonzalo Tejeda, Andrew B Tobin, Massimo Vassalli, Anthony J Chalmers, Jinendra Ekanayake, Daniele Faccio

{"title":"Wide-field and non-invasive imaging of brain tumours with scattered light techniques.","authors":"Philip Binner, Jack Radford, Ilya Starshynov, Mansa Madhusudan, Karen Strathdee, Katrina Stevenson, Matthew Walker, Giuseppe Ciccone, Gonzalo Tejeda, Andrew B Tobin, Massimo Vassalli, Anthony J Chalmers, Jinendra Ekanayake, Daniele Faccio","doi":"10.1364/BOE.587407","DOIUrl":"https://doi.org/10.1364/BOE.587407","url":null,"abstract":"The ability to identify tumour tissue in a label-free, contactless, and real-time manner is much needed in tumour resection surgery. Current techniques cause interruptions to surgical flow and have high false positive rates, which can cause collateral damage to healthy brain tissue. We propose laser light scattering techniques, such as diffuse correlation spectroscopy and laser speckle contrast imaging, to image mechanical stiffness differences in the brain's surface associated with tumour tissue. We validate the optimal processing technique quantitatively with a controlled experiment in which paraformaldehyde was used to induce a change in tissue stiffness in ex vivo mouse brains. We then demonstrate that the technique applies to tumour localisation using ex vivo mouse models with real tumours. Qualitative comparisons with magnetic resonance imaging indicate accurate tumour localisation using only surface stiffness changes to underlying tumours. We also demonstrate sub-millimetre precision when imaging brain slices.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 3","pages":"1112-1124"},"PeriodicalIF":3.2,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13064597/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147670270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FPM2Stain Net: physics-guided super-resolution and multi-modal virtual staining for digital histopathology. FPM2Stain网：物理引导的超分辨率和多模态虚拟染色用于数字组织病理学。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-01-30 eCollection Date: 2026-02-01 DOI: 10.1364/BOE.586327

Qijun Yang, Lintao Xiang, Chang Bian, Yating Huang, Hongpei Zheng, Hujun Yin

{"title":"FPM2Stain Net: physics-guided super-resolution and multi-modal virtual staining for digital histopathology.","authors":"Qijun Yang, Lintao Xiang, Chang Bian, Yating Huang, Hongpei Zheng, Hujun Yin","doi":"10.1364/BOE.586327","DOIUrl":"10.1364/BOE.586327","url":null,"abstract":"We propose FPM2Stain Net, a jointly optimized end-to-end computational pipeline that integrates physics-guided super-resolution with deep learning-based virtual staining to enable high-resolution, multi-modal digital histopathology. The first stage, bidirectional physics-based Fourier ptychographic microscopy (BiP-FPM), reconstructs high-resolution amplitude and phase maps from a multi-LED FPM image stack using a self-supervised ResNet-U-Net guided by a differentiable point spread function (PSF) model with learnable pupil correction and bidirectional physics-consistent constraints. The second stage employs a multi-task conditional generative adversarial network (cGAN) enhanced with wavelet-based spatial-frequency fusion and perceptual supervision to synthesize clinically relevant staining modalities, including H&E, DAPI, LAP2, and panCK. Extensive experiments on both simulated and real tissue datasets demonstrate that FPM2Stain Net outperforms conventional FPM, GAN-based methods, and diffusion-based models in both reconstruction fidelity and staining accuracy. The synthesized virtual stains not only preserve fine structural details but also enable downstream analysis, such as cell segmentation and biomarker quantification, with higher accuracy than using images acquired with a conventional 40× objective microscope. These results confirm that FPM2Stain Net achieves a greater-than-10× pixel-level upsampling factor relative to the low-magnification (4×) input image, and provides a fast, scalable, and cost-effective alternative to chemical staining in digital pathology, multiplex imaging, and point-of-care diagnostics.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 2","pages":"1074-1097"},"PeriodicalIF":3.2,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12904525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146200135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatially-resolved optical monitoring of bioreactor cell growth. 生物反应器细胞生长的空间分辨光学监测。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-01-30 eCollection Date: 2026-02-01 DOI: 10.1364/BOE.583724

J Gorecki, C Redwood-Sawyerr, J Cao, H Dehghani, C Kontoravdi, K Polizzi, C Rowlands

{"title":"Spatially-resolved optical monitoring of bioreactor cell growth.","authors":"J Gorecki, C Redwood-Sawyerr, J Cao, H Dehghani, C Kontoravdi, K Polizzi, C Rowlands","doi":"10.1364/BOE.583724","DOIUrl":"10.1364/BOE.583724","url":null,"abstract":"Bioreactors are used for the industrial-scale culture of cells to obtain valuable products such as pharmaceuticals, enzymes, and biofuels; however, monitoring the growth conditions within the vessels is challenging and is often dependent on single-point ex-situ measurements. Further, spatial heterogeneities are known to exist within these environments, thereby creating regions of low growth or incomplete reactions, reducing yield, and importantly, reducing the applicability of single-point measurement methods. Optical imaging is an attractive method for remote spatially-resolved measurement platforms; however, the strong optical scattering within cell cultures makes imaging almost impossible. Here, we utilise this parasitic scattering effect and present a spatially resolved optical method for monitoring cell density within a bioreactor, using optical measurement of local scattering parameters as a proxy measurement for cell density. Our method is non-invasive and does not require the removal of any cell material from within the vessel. We propose that our optical measurement method can be incorporated into process-control feedback systems, providing insightful information on cell growth that can be used to deliver higher spatial homogeneity and increased yields.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 2","pages":"1098-1111"},"PeriodicalIF":3.2,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12904529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146199810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visual appearance of blood vessels: a phantom study. 血管的视觉外观：一项幻影研究。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-01-29 eCollection Date: 2026-02-01 DOI: 10.1364/BOE.579246

Markus Wagner, David Hevisov, Corinna Konrad, Hannes Schmidt, Florian Foschum, Alwin Kienle

引用次数: 0

Thermal lensing effects in two-photon light-sheet microscopy. 双光子光片显微镜中的热透镜效应。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-01-29 eCollection Date: 2026-02-01 DOI: 10.1364/BOE.564339

Antoine Hubert, Hugo Trentesaux, Thomas Pujol, Georges Debrégeas, Volker Bormuth

{"title":"Thermal lensing effects in two-photon light-sheet microscopy.","authors":"Antoine Hubert, Hugo Trentesaux, Thomas Pujol, Georges Debrégeas, Volker Bormuth","doi":"10.1364/BOE.564339","DOIUrl":"10.1364/BOE.564339","url":null,"abstract":"In light-sheet fluorescence microscopy (LSFM), the axial resolution is governed by the illumination beam profile, motivating the development of advanced beam-shaping techniques to enhance imaging performance. Two-photon LSFM (2P-LSFM), in particular, improves the signal-to-background ratio by reducing laser scattering and distortion in biological specimens. However, we report a potentially detrimental thermal effect in 2P-LSFM: the high laser powers required for two-photon excitation induce localized heating, which alters the refractive index of the medium and effectively forms a divergent thermal lens in water. At 500 mW, the light-sheet waist broadens by 25% and shifts by 300 μm before stabilizing several seconds after the laser shutter is opened. Both experiments and simulations reveal that this thermal lensing effect scales with laser power and the path length the beam travels through water. The resulting degradation in resolution and signal-to-noise ratio may compromise imaging applications that require high laser powers for rapid volumetric imaging of large specimens or functional brain imaging. This limitation is particularly critical in dynamic sample environments, such as during stepwise repositioning or flow-based delivery of chemical or hydrodynamic sensory stimuli, where changes occur on timescales comparable to the thermal settling time.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 2","pages":"1064-1073"},"PeriodicalIF":3.2,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12904557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146199859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-free tracking of subcortical white matter degradation in vivo using third harmonic generation microscopy in a mouse model of multiple sclerosis. 在多发性硬化症小鼠模型中，使用三次谐波显微镜对体内皮层下白质降解进行无标记跟踪。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-01-29 eCollection Date: 2026-02-01 DOI: 10.1364/BOE.576538

Nicole E Chernavsky, Nuri Hong, Lianne J Trigiani, Nozomi Nishimura, Chris B Schaffer

引用次数: 0

Two-lens telecentric model eyes for image distortion measurement in adaptive optics ophthalmoscopes. 用于自适应光学检眼镜图像畸变测量的双透镜远心模型眼。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-01-29 eCollection Date: 2026-02-01 DOI: 10.1364/BOE.582188

Yuning Xia, Gastón A Ayubi, Julie Bentley, Alfredo Dubra

{"title":"Two-lens telecentric model eyes for image distortion measurement in adaptive optics ophthalmoscopes.","authors":"Yuning Xia, Gastón A Ayubi, Julie Bentley, Alfredo Dubra","doi":"10.1364/BOE.582188","DOIUrl":"10.1364/BOE.582188","url":null,"abstract":"Here we present the design of telecentric model eyes for measuring image distortion in adaptive optics ophthalmoscopes, using pairs of achromatic or near-achromatic commercial off-the-shelf (COTS) and/or custom doublet lenses. The proposed model eyes can operate over visible or visible plus near infrared wavelength ranges, across a 15-diopter focus range, with an 8 mm entrance pupil diameter. COTS lens selection was implemented as a systematic evaluation of catalog lenses after optimization of axial distances. Custom lens optimization was initially guided by wavefront aberration constraints derived from object-shift 3rd order aberration theory, before traditional variation of surfaces and distances to minimize a performance metric. When a wavefront corrector compensates for field-constant aberrations, the model eyes achieve nominal wavefront RMS below λ/20 across a ∼6.9° circular full field of view for COTS and hybrid lens pairs, limited by vignetting from a self-imposed 27.9 mm clear aperture. For custom lens pairs, the field of view is about 10.2°, limited by aberrations. A tolerance study indicates that a COTS lens pair for 450-1100 nm can achieve as-built diffraction-limited wavefront error of 0.044 λ @450 nm at 95% production yield. Model eyes with this lens pair yielded telecentricity quantified as image size changed per diopter of 0.1%/D when assembled with a camera and 0.01%/D when assembled with a power meter. The model eye was tested with an optical setup that emulates a fundus camera with Badal focus correction.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 2","pages":"1029-1048"},"PeriodicalIF":3.2,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12904538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146200042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blood flow analysis of retinal neovascularisations in a VLDLR mouse model using contrast-enhanced optical coherence tomography. 使用对比度增强光学相干断层扫描对VLDLR小鼠模型视网膜新生血管的血流分析。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-01-28 eCollection Date: 2026-02-01 DOI: 10.1364/BOE.574030

Yash Patel, Bernhard Baumann, Conrad Merkle

{"title":"Blood flow analysis of retinal neovascularisations in a VLDLR mouse model using contrast-enhanced optical coherence tomography.","authors":"Yash Patel, Bernhard Baumann, Conrad Merkle","doi":"10.1364/BOE.574030","DOIUrl":"10.1364/BOE.574030","url":null,"abstract":"Age-related macular degeneration (AMD) is a major cause of global blindness that affects millions worldwide. Certain forms of AMD cause neovascularisations (NVs), which form retinal-choroidal anastomoses. This disrupts healthy haemodynamic patterns, and early detection and treatment are crucial for preserving vision. Here, we employ a custom-built optical coherence tomography (OCT) imaging system to investigate these NVs in a very low-density lipoprotein receptor (VLDLR) knockout mouse model. Mice were imaged before, during, and after contrast agent injection, aiming to enhance our understanding of the NV haemodynamics. Doppler signal analysis techniques were employed to calculate flow velocities within individual NVs. Flow rates pre- and post-injection were determined based on these velocity measurements. Particle tracking was performed on two NVs for a comparative analysis with the Doppler velocity measurements. Both methods of measuring flow velocities showed good agreement post-contrast injection. The analysis of post-injection flow rates from the NVs revealed diverse behaviours. Some NVs exhibited stable flow rates over time, while others showed signs of instability, with flow rates changing substantially or even changing flow direction at different time points. Additionally, it was observed at multiple time points that flow from certain NVs moved from the choroid to the retina at the same time that other NVs displayed flow in the opposite direction. These observations suggest complex interactions between choroidal and retinal vascular networks in diseases like AMD. Further characterisation using contrast-enhanced Doppler OCT may improve our understanding of neovascular haemodynamics.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 2","pages":"991-1010"},"PeriodicalIF":3.2,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12904560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146200146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predicting wrist osteoporosis from excised human finger bones using spatially offset Raman spectroscopy - a cadaveric study. 利用空间偏移拉曼光谱从切除的人类指骨预测手腕骨质疏松症-一项尸体研究。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-01-27 eCollection Date: 2026-02-01 DOI: 10.1364/BOE.583075

Mohammad Hosseini, Sadia Afrin, Anthony Yosick, Emma Schenker, Hani Awad, Andrew J Berger

{"title":"Predicting wrist osteoporosis from excised human finger bones using spatially offset Raman spectroscopy - a cadaveric study.","authors":"Mohammad Hosseini, Sadia Afrin, Anthony Yosick, Emma Schenker, Hani Awad, Andrew J Berger","doi":"10.1364/BOE.583075","DOIUrl":"10.1364/BOE.583075","url":null,"abstract":"Osteoporosis and osteopenia remain vastly underdiagnosed. Current clinical screening relies almost exclusively on dual-energy X-ray absorptiometry (DXA), which measures bone mineral density (BMD) but fails to capture the compositional changes that lead to BMD loss. We investigated whether spatially offset Raman spectroscopy (SORS) applied to excised finger bones can assess subsurface biochemical markers associated with bone health and estimate wrist DXA T-scores. Raman spectra were acquired ex vivo on the mid-shaft of the proximal phalanx of the second digit from 25 female cadavers spanning the three T-score categories (n = 8 normal, n = 6 osteopenic, and n = 11 osteoporotic) at spatial offsets of 0, 3, and 6 mm from a laser irradiation spot. After normalizing spectra to the PO4 3- peak, group-averaged spectra of the three categories, measured at 3-mm offset, showed clear differences in the CO3 2-, Amide III, CH2, and Amide I bands. Quantitatively, four out of five mineral-to-matrix ratios discriminated (p ≤ 0.05) osteopenia from both normal and osteoporotic bone, and all five ratios showed significant differences between normal and osteoporotic bone. In contrast, the 0-mm offset showed reduced biochemical contrast in univariate analyses, while the 6-mm offset did not provide additional discrimination relative to 3-mm. Multivariate partial-least-squares regression (PLSR) models using 0-mm and 3-mm spectra predicted distal radius T-scores with comparable accuracy (Pearson r ≈ 0.90, RMSECV ≈ 0.8), but the 3-mm offset required fewer latent variables (1 vs. 9), consistent with the univariate analysis trends favoring 3-mm offset. These excised-bone findings justify future studies extending this approach to transcutaneous fingers for non-ionizing assessment of bone health.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 2","pages":"977-990"},"PeriodicalIF":3.2,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12904550/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146199527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescent properties of FDA-approved anti-leukemia drugs. fda批准的抗白血病药物的荧光特性。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-01-26 eCollection Date: 2026-02-01 DOI: 10.1364/BOE.580253

Dóra Bereczki, Ines Lidia Haffaressas, Zoltán Szabó, Szilárd Tóth, András Füredi, Péter Fürjes

{"title":"Fluorescent properties of FDA-approved anti-leukemia drugs.","authors":"Dóra Bereczki, Ines Lidia Haffaressas, Zoltán Szabó, Szilárd Tóth, András Füredi, Péter Fürjes","doi":"10.1364/BOE.580253","DOIUrl":"10.1364/BOE.580253","url":null,"abstract":"The inherent fluorescent properties of anti-leukemic drugs offer unique advantages for real-time therapeutic tracking and optimization. In this study, we systematically screened the absorption and emission spectra of 82 leukemia-related compounds, identifying 28 autofluorescent drugs suitable for fluorescence-based optical concentration monitoring. Excitation and emission parameters were evaluated across various solvents (DMSO, fetal bovine serum, and culture media), revealing solvent-dependent spectral changes, intensity variations, and effect on detection limits. These 28 compounds were further assessed for cytotoxicity screening in case of drug-naive and drug-resistant K562 leukemia lymphoblast cells. By correlating their spectral properties with cytotoxic responses, our study establishes a robust framework for fluorescence-assisted drug profiling, enabling pharmacokinetic insights, resistance prediction, and informed therapeutic adjustments. These findings underscore the translational potential of fluorescence-based methodologies in supporting precision medicine for leukemia treatment.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 2","pages":"963-976"},"PeriodicalIF":3.2,"publicationDate":"2026-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12904533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146200120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0