Biomedical optics express最新文献

{"title":"Multispectral photoacoustic imaging of breast cancer tissue with histopathology validation.","authors":"Junhao Zhang, Junior Arroyo, Muyinatu A Lediju Bell","doi":"10.1364/BOE.547262","DOIUrl":"10.1364/BOE.547262","url":null,"abstract":"<p><p>Intraoperative multispectral photoacoustic pathology assessment presents a promising approach to guide biopsy resection. In this study, we developed and validated a novel photoacoustic technique to differentiate between healthy and cancerous tissues. Our method consisted of photoacoustic contrast calculations as a function of wavelength, followed by projections of the resulting spectra from training data into a two-dimensional space using principal component analysis to create representative spectra, then calculation of the average cosine similarity between the spectrum of each pixel in test data and the representative spectra. The test healthy tissue region had a 0.967 mean correlation with the representative healthy tissue spectrum and a lower mean correlation (0.801) with the cancer tissue spectrum. The test cancer tissue region had a 0.954 mean correlation with the cancer tissue spectrum and a lower mean correlation (0.762) with the healthy tissue spectrum. Our method was further validated through qualitative comparison with high-resolution hematoxylin and eosin histopathology scans. Healthy tissue was primarily correlated with the optical absorption of blood (i.e., deoxyhemoglobin), while invasive ductal carcinoma breast cancer tissue was primarily correlated with the optical absorption of lipids. Our label-free histopathology approach utilizing multispectral photoacoustic imaging has the potential to enable real-time tumor margin determination during biopsy or surgery.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"995-1005"},"PeriodicalIF":2.9,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919340/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and characterization of an optical phantom for mesoscopic multimodal fluorescence lifetime imaging and optical coherence elastography.","authors":"Luis Chavez, Shan Gao, Vikas Pandey, Nanxue Yuan, Saif Ragab, Jiayue Li, Matt S Hepburn, Percy Smith, Caroline Edelheit, David T Corr, Brendan F Kennedy, Xavier Intes","doi":"10.1364/BOE.549695","DOIUrl":"10.1364/BOE.549695","url":null,"abstract":"<p><p>We developed a novel methodology for manufacturing multimodal, tissue-mimicking phantoms that exhibit both molecular and biomechanical contrast. This methodology leverages the immiscibility of silicone and hydrogels to create solid mesoscale phantoms with localized regions of precisely controlled fluorescence, including fluorescence lifetime properties, and adjustable stiffness, without requiring physical barriers. Mechanical, fluorescent, and optical characterization confirmed the tunability of the phantoms across a range of values relevant to biomedical applications. A macroscale 3D phantom was fabricated, and its properties were validated through fluorescence lifetime imaging (FLI) and optical coherence elastography (OCE). Validation demonstrated the successful tuning of both mechanical and fluorescence lifetime contrasts within a 3D structure, highlighting the feasibility of multimodal FLI-OCE. This new phantom manufacturing process is expected to support the development and validation of new multimodal imaging approaches to study molecular and biomechanical properties of the tumor microenvironment (TME), as well as their impact on therapeutic efficacy, and to enhance targeted therapies.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"1006-1024"},"PeriodicalIF":2.9,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electro-active evanescent-wave cavity ring-down spectroscopy immunosensor for influenza virus detection.","authors":"Shadi A Alnaanah, Aymen H Qatamin, Sergio B Mendes, Martin G O'Toole, Betty M Nunn, Mohammad S Zannon","doi":"10.1364/BOE.554668","DOIUrl":"10.1364/BOE.554668","url":null,"abstract":"<p><p>The early and accurate detection of viral pathogens is critical for effective disease management and public health safety. This study introduces an immunosensor that integrates an electro-active evanescent-wave cavity ring-down spectroscopy (EW-CRDS) platform with a sandwich-type bioassay for label-free detection of the influenza A (H5N1) hemagglutinin (HA) protein, achieving a detection limit of 15 ng/mL. The sensor is constructed by functionalizing the EW-CRDS platform within a micro-electrochemical flow cell with a monoclonal antibody specific to the target antigen. Upon antigen binding, a secondary polyclonal antibody conjugated with a redox-active methylene blue dye is captured. This dye undergoes reversible optical signal changes during redox transitions, which are electrochemically modulated and detected with high sensitivity. Unlike conventional approaches, this sensor employs electrochemical modulation to amplify surface-specific optical signals while reducing processing time and minimizing background noise. The results demonstrate the potential of this technology for real-time monitoring and rapid, reliable diagnosis of infectious diseases, offering excellent sensitivity and ease of operation in detecting influenza viruses. This work highlights the promise of the electro-active EW-CRDS platform for antigen detection in clinical settings.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"982-994"},"PeriodicalIF":2.9,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid, ultrasensitive, and specific RPA-THz system for pathogenic microorganism detection.","authors":"Xupeng Fu, Lintao Huang, Ying Chen, Menglu Pi, Lin Ma, Hu Cai, Xuehao Wang, Zhihao Chen, Hang Shi, Wenhui Yang, Fulai Zhang, Yang Zhang, Huili Jiang, Zeming Zhou, Changhe Wang, Rong Huang, Juan Zhang, Donghao Cheng, Li-An Wu, Airong Qian, Ye Tian","doi":"10.1364/BOE.549870","DOIUrl":"10.1364/BOE.549870","url":null,"abstract":"<p><p>Pathogenic microorganisms responsible for infectious diseases pose a significant global threat to human health. Existing detection methods, such as qPCR and ELISA, fail to simultaneously meet the requirements for high sensitivity, high specificity, and rapid detection. This study presents an innovative approach for the rapid, specific, and highly sensitive detection of pathogenic microorganisms, particularly <i>Escherichia coli</i> O157:H7 (<i>E. coli</i> O157:H7) and varicella-zoster virus (VZV), by combining recombinase polymerase amplification (RPA) with terahertz time-domain spectroscopy (THz-TDS). The qualitative and quantitative detection method for pathogenic microorganisms was developed and evaluated. The stable and efficient RPA reaction systems were established to specifically amplify the key conserved genes of these pathogens. Then the RPA products were purified, and enriched with MBs. The absorbance spectra were obtained using THz-TDS technology. The linear range of the RPA-THz for detecting <i>E. coli</i> O157:H7 was 0.55 to 5.5 × 10⁴ pg/mL, while for VZV, it was 0.75 to 7.5 × 10³ pg/mL. The limit of detection (LOD) for bacteria and viruses was 0.226 pg/mL and 0.528 pg/mL, respectively, demonstrating better sensitivity than the qPCR (550 pg/mL and 750 pg/mL, respectively). In addition, the whole amplification and detection process was completed in about 35 minutes. Compared to traditional pathogen detection techniques, the primary advantage of the developed RPA-THz method exhibited high accuracy, good reproducibility, and short detection times, enabling non-ionizing, label-free analysis for rapid detection with high sensitivity and specificity of pathogenic microorganisms. This study provides a theoretical foundation and practical demonstration for the fast and precise detection of pathogenic microorganisms. It establishes a crucial research basis for further development of RPA-THz sensors, advancing technological progress in the field of food safety, medical diagnostics, environmental monitoring, and public health.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"949-964"},"PeriodicalIF":2.9,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal imaging-based core peripheral temperature difference measurement for neonatal monitoring in the NICU.","authors":"Nantao Zhang, Xiaoyan Song, Junli He, Fengchao Liang, Jie Yang, Wenjin Wang","doi":"10.1364/BOE.549693","DOIUrl":"10.1364/BOE.549693","url":null,"abstract":"<p><p>The core-peripheral temperature difference (CPTD) refers to the difference between the body's core temperature (e.g., chest or abdomen) and peripheral skin temperature (e.g., hands or feet). It serves as a key biomarker for assessing the hemodynamic status of newborns and is an important early warning indicator of potential shock and severe infection. Measurement of CPTD in clinical practice currently requires the use of an infrared spot thermometer to measure the temperature of multiple body parts of a neonate, which is not possible for continuous and fully automatic long-term monitoring. To address these limitations, we propose a thermal infrared (TIR)-based approach that enables non-contact, fully automatic, and continuous CPTD measurement for neonates. The spatial redundancy property of TIR is utilised and combined with a deep learning-based body parsing model to automatically detect different body parts of a neonate, including the chest and limbs (e.g., hand or foot), and measure the temperatures of these two parts to derive their difference as CPTD. Although accurate measurement of the absolute temperature of the neonatal skin is difficult due to the calibration of the TIR camera and environmental influence, the temperature difference between different body parts that emphasizes the spatial contrast at certain moments can be reliably estimated, and it is independent of the subject and environment. In a prospective clinical trial involving 40 preterm infants, our TIR-based CPTD measurement showed a mean absolute error less than <math><msup><mi>0.3</mi> <mo>∘</mo></msup> </math> C. Additionally, hand temperatures were, on average, 1.11°C higher than foot temperatures.Hand temperatures also showed a more pronounced response to changes in core temperature, suggesting that they may be better indicators of fluctuations in core temperature. Finally, we investigated the relationship between TIR-based CPTD and infant circulatory disorders. We find that infants with circulatory disorders typically have higher CPTD values, which demonstrates the clinical potential of our methods in reflecting functional limitations of the circulatory system in newborns. To our knowledge, this is the first clinical showcase of using a TIR camera for continuous non-contact CPTD monitoring of preterm infants in the hospital neonatal intensive care unit (NICU), providing important preliminary findings that may enrich the video health monitoring applications in the NICU.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"965-981"},"PeriodicalIF":2.9,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-modality SEM-Raman smart scanning for fast hyperspectral Raman micro-imaging - application to bones.","authors":"Valentin Gilet, Guillaume Mabilleau, Matthieu Loumaigne, Raffaele Vitale, Thomas Oberlin, José Henrique de Morais Goulart, Nicolas Dobigeon, Cyril Ruckebusch, David Rousseau","doi":"10.1364/BOE.551298","DOIUrl":"10.1364/BOE.551298","url":null,"abstract":"<p><p>Recent works on smart scanning techniques in Raman micro-imaging demonstrate the possibility of highly reducing acquisition time. In particular, Gilet <i>et al.</i> [Optics Express32, 932 (2024)10.1364/OE.509736] proposed a protocol combining compression in both spectral and spatial domains by focusing on essential information. This protocol consists of a two-pass scan in Raman modality at different signal-to-noise ratios (SNR). The first scan of the entire sample area at low SNR, and was identified as the bottleneck of the whole process. We propose revisiting this protocol by replacing this first scan with scanning electron microscopy (SEM), which is a faster imaging modality. We demonstrate that acquiring real data of biomedical interest according to this new protocol is three times faster, with limited distortion on the reconstructed Raman spectra and preserved clinical value of the extracted information. This is illustrated on bone samples for which SEM is correlated with Raman. We discuss the potential extension of this method to other slow spectral imaging modalities conventionally based on raster scans.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"935-948"},"PeriodicalIF":2.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optical analysis for effective phototherapy in an LED oral care device.","authors":"Younghoon Shin, Woosub Song, In Hee Shin, Dae Won Ji, Kyoung Jae Min, Sun-Hee Ahn","doi":"10.1364/BOE.553671","DOIUrl":"10.1364/BOE.553671","url":null,"abstract":"<p><p>This paper investigates the advanced capabilities of light-emitting diodes (LEDs) in oral care devices, emphasizing their versatility in wavelength control and ability to reach complex areas within the oral cavity. While LEDs enable precise dosage control and adjustable penetration depths, existing oral care devices are often limited to single-wavelength designs, primarily targeting anterior teeth whitening or lateral surfaces, thereby failing to provide comprehensive oral coverage. To address these limitations, this study introduces a novel LED-based oral care device integrating three distinct wavelengths: blue for antibacterial effects, green for anti-inflammatory effects, and red for preventive and therapeutic applications. Using computed tomography (CT) data, upper and lower dental arch trajectories were acquired to design a flexible printed circuit board (FPCB) that conforms to the natural curvature of the dental arch. Strategically placed LEDs on the FPCB ensure uniform light distribution and optimized irradiance across the entire oral cavity. This research systematically determines the optimal design parameters and operating conditions necessary for achieving appropriate irradiance density, including LED placement, operating time, and power control through driving current and duty cycles. The findings demonstrate a practical and effective approach to overcoming the current limitations of LED oral care devices, significantly enhancing their performance and applicability in dental phototherapy.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"922-934"},"PeriodicalIF":2.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OAH-Net: a deep neural network for efficient and robust hologram reconstruction for off-axis digital holographic microscopy.","authors":"Wei Liu, Kerem Delikoyun, Qianyu Chen, Alperen Yildiz, Si Ko Myo, Win Sen Kuan, John Tshon Yit Soong, Matthew Edward Cove, Oliver Hayden, Hwee Kuan Lee","doi":"10.1364/BOE.547292","DOIUrl":"10.1364/BOE.547292","url":null,"abstract":"<p><p>Off-axis digital holographic microscopy is a high-throughput, label-free imaging technology that provides three-dimensional, high-resolution information about samples, which is particularly useful in large-scale cellular imaging. However, the hologram reconstruction process poses a significant bottleneck for timely data analysis. To address this challenge, we propose a novel reconstruction approach that integrates deep learning with the physical principles of off-axis holography. We initialized part of the network weights based on the physical principle and then fine-tuned them via supersized learning. Our off-axis hologram network (OAH-Net) retrieves phase and amplitude images with errors that fall within the measurement error range attributable to hardware, and its reconstruction speed significantly surpasses the microscope's acquisition rate. Crucially, OAH-Net, trained and validated on diluted whole blood samples, demonstrates remarkable external generalization capabilities on unseen samples with distinct patterns. Additionally, it can be seamlessly integrated with other models for downstream tasks, enabling end-to-end real-time hologram analysis. This capability further expands off-axis holography's applications in both biological and medical studies.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"894-909"},"PeriodicalIF":2.9,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Super-resolution microscopy based on the inherent fluctuations of dye molecules.","authors":"Alexander Krupinski-Ptaszek, Adrian Makowski, Aleksandra Mielnicka, Monika Pawłowska, Ron Tenne, Radek Lapkiewicz","doi":"10.1364/BOE.533263","DOIUrl":"10.1364/BOE.533263","url":null,"abstract":"<p><p>Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization microscopy and stimulated-emission-depletion microscopy often demand considerable resources. These methods depend heavily on elaborate sample-staining, complex optical systems, or prolonged acquisition periods, and their application in 3D and multicolor imaging presents significant experimental challenges. In the current work, we provide a complete demonstration of a widely accessible super-resolution imaging approach capable of 3D and multicolor imaging based on super-resolution optical fluctuation imaging (SOFI). We replace the confocal pinhole with an array of single-photon avalanche diodes and use the microsecond-scale fluctuations of dye molecules as a contrast mechanism. This contrast is transformed into a super-resolved image using a robust and deterministic algorithm. Our technique utilizes natural fluctuations inherent to organic dyes, thereby it does not require engineering of the blinking statistics. Our robust, versatile super-resolution method opens the way to next-generation multimodal imaging and facilitates on-demand super-resolution within a confocal architecture.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"910-921"},"PeriodicalIF":2.9,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of focus-tunable presbyopia correction lenses operated using gaze-tracking and LIDAR.","authors":"Rajat Agarwala, Björn R Severitt, Felix F Reichel, Benedikt W Hosp, Siegfried Wahl","doi":"10.1364/BOE.543807","DOIUrl":"10.1364/BOE.543807","url":null,"abstract":"<p><p>Presbyopia is an age-related loss of accommodation ability of the eye which affects an individual's capacity to focus on closer objects. With the advent of tunable lens technologies, various algorithms have been developed to tune such lenses for presbyopia correction in older populations. In this study, we assessed a gaze and LIDAR-based feedback mechanism with electronically tunable lenses for their use as correction lenses for presbyopia. The tunable lens prototype was evaluated in 15 healthy young participants with their corrected sphero-cylindrical refraction by comparing their performance for a dynamic matching task under two conditions: (1) natural accommodation, and (2) emulating presbyopia using cycloplegic drops to paralyse accommodation while focussing using the developed visual demonstrator prototype. The participants performed the matching task on three screens placed at multiple distances. We have demonstrated that gaze can be used in conjunction with LIDAR to tune the lenses in the wearable visual demonstrator prototype, enabling participants to achieve a fast and accurate response for the matching task.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"883-893"},"PeriodicalIF":2.9,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}