Biomedical optics express最新文献_第3页

Highly parallel, 1060 nm interferometric diffusing wave spectroscopy with a time-of-flight filter. 高度平行，1060nm干涉漫射波光谱与飞行时间滤波器。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-02-10 eCollection Date: 2026-03-01 DOI: 10.1364/BOE.576865

Santosh Aparanji, Mingjun Zhao, Akshay Shashidhar Nadig, Hector Garcia Estrada, Drew Hamilton, Vivek J Srinivasan

{"title":"Highly parallel, 1060 nm interferometric diffusing wave spectroscopy with a time-of-flight filter.","authors":"Santosh Aparanji, Mingjun Zhao, Akshay Shashidhar Nadig, Hector Garcia Estrada, Drew Hamilton, Vivek J Srinivasan","doi":"10.1364/BOE.576865","DOIUrl":"https://doi.org/10.1364/BOE.576865","url":null,"abstract":"Interferometric diffuse optics (iDO) has recently emerged as a promising class of near-infrared (NIR) light technologies for monitoring human brain signals associated with coherent light fluctuations. In this work, we demonstrate a line scan interferometric diffusing wave spectroscopy (iDWS) system at 1060 nm, a wavelength that has a multitude of benefits for high-speed cerebral blood flow index (BFI) monitoring. Pulsatile BFI measurements on the forehead of a moderately dark-skinned (Fitzpatrick Type V) subject with medium-length black hair up to a source-collector (S-C) separation of 5.5 cm on the forehead and 4.0 cm over the parietal cortex are demonstrated in continuous wave (CW) mode. On this high-throughput platform, we further implement a simple time-of-flight filter (TOF) via source wavelength tuning. The TOF filter can be turned on and off, and its width can be changed electronically, enabling probing different sample depths without requiring multiple S-C separations. At 4 cm S-C separation, an electronic TOF filter afforded a 2.92-fold reduction in scalp sensitivity over CW mode. With further optimization, the combination of TOF filtering with highly parallel detection in the 1060 nm range promises to improve depth sensitivity and signal-to-noise ratio of iDO in neuromonitoring applications.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 3","pages":"1241-1254"},"PeriodicalIF":3.2,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13064615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147670178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tartrazine-enhanced visible-light OCT for deep-tissue imaging. 酒石黄增强可见光OCT用于深部组织成像。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-02-10 eCollection Date: 2026-03-01 DOI: 10.1364/BOE.585266

Jiaxuan Kong, Lingyun Wang, Shaohua Pi

引用次数: 0

Circular-scanning variance delay and sum algorithm for photoacoustic imaging. 光声成像的循环扫描方差延迟和算法。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-02-09 eCollection Date: 2026-03-01 DOI: 10.1364/BOE.577910

Zhicheng Wang, Zijun Xi, Qian Song, Xiong Wang

引用次数: 0

Semi-supervised 3D diabetic macular edema segmentation in OCT volumes via independent dual-branch consistency learning. 基于独立双分支一致性学习的半监督三维糖尿病黄斑水肿分割。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-02-09 eCollection Date: 2026-03-01 DOI: 10.1364/BOE.584054

Zitong Liu, Kaiming Li, Shuaichen Lin, Mohan Qin, Renxiong Wu, Yunchun Zou, Jie Zhong, Yong Liu, Guangming Ni

{"title":"Semi-supervised 3D diabetic macular edema segmentation in OCT volumes via independent dual-branch consistency learning.","authors":"Zitong Liu, Kaiming Li, Shuaichen Lin, Mohan Qin, Renxiong Wu, Yunchun Zou, Jie Zhong, Yong Liu, Guangming Ni","doi":"10.1364/BOE.584054","DOIUrl":"https://doi.org/10.1364/BOE.584054","url":null,"abstract":"Accurate 3D lesion segmentation in optical coherence tomography (OCT) images is crucial for the detection and treatment evaluation of diabetic macular edema (DME), but is hindered by scarce labeled data and complex boundaries. To address these challenges, we propose a semi-supervised 3D DME segmentation method that leverages a dual independent-branch co-training framework with mask perturbation consistency. The dual branches jointly exploit complementary multi-view features, while an independence-measuring strategy encourages parameter divergence to break performance bottlenecks. To improve robustness against diverse lesion morphologies, we introduce dynamic region-mask augmentation and a bidirectional confidence filtering mechanism, enforcing strong consistency constraints between the two branches. Additionally, a lightweight channel-attention module integrates multi-scale context. Extensive experiments demonstrate that our method surpasses state-of-the-art approaches, validating its effectiveness and superiority.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 3","pages":"1223-1240"},"PeriodicalIF":3.2,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13064604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147670252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the angiogenic potential of xenografted tumors by biomedical imaging techniques. 生物医学成像技术评估异种移植肿瘤血管生成潜能。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-02-05 eCollection Date: 2026-03-01 DOI: 10.1364/BOE.577775

Irina Druzhkova, Anna Orlova, Pavel Subochev, Alexey Kurnikov, Anna Glyavina, Alina Isakova, Ekaterina Kukovyakina, Ekaterina Plotnikova, Marine Gasparian, Anastasia Komarova, Snezhana Sinyushkina, Arseniy Potapov, Ruslan Spashchanskii, Anastasia Anina, Mikhail Kirpichnikov, Dmitry Dolgikh, Daniel Razansky, Anne Yagolovich

{"title":"Assessment of the angiogenic potential of xenografted tumors by biomedical imaging techniques.","authors":"Irina Druzhkova, Anna Orlova, Pavel Subochev, Alexey Kurnikov, Anna Glyavina, Alina Isakova, Ekaterina Kukovyakina, Ekaterina Plotnikova, Marine Gasparian, Anastasia Komarova, Snezhana Sinyushkina, Arseniy Potapov, Ruslan Spashchanskii, Anastasia Anina, Mikhail Kirpichnikov, Dmitry Dolgikh, Daniel Razansky, Anne Yagolovich","doi":"10.1364/BOE.577775","DOIUrl":"https://doi.org/10.1364/BOE.577775","url":null,"abstract":"Tumor angiogenesis promotes tumor growth, metastasis and disease progression. Different cancer types vary in their angiogenic potential, which may influence prognosis and response to therapy. In the present work, we established xenograft models of three of the most aggressive types of human cancers: glioblastoma U87MG, gastric cancer MKN-45, and pancreatic cancer MIA PaCa-2, in immunodeficient mice. The study of vascular network by optoacoustic microangiography revealed the highest degree of vascularization in U87MG xenografts, and the lowest in MIA PaCa-2 xenografts. As shown by PAS-CD31 dual staining, U87MG-derived tumors also showed the highest expression of the endothelial marker CD31 as well as the highest vasculogenic mimicry capacity. In line with this, metabolic imaging by fluorescence Lifetime Imaging Microscopy (FLIM) of nicotinamide adenine dinucleotide (NADH) revealed that MIA PaCa-2 xenografts were the most glycolytic, whereas U87MG had higher levels of oxidative phosphorylation, and MKN-45 showed intermediate values. Therefore, when creating animal models with xenografted tumors, it is important to understand the angiogenic potential of cancer cells, especially for studying drug candidates with an antiangiogenic effect. Also, the combination of optoacoustics and immunohistochemical analysis with FLIM imaging allows for a comprehensive assessment of both vascularization and the metabolic state of the tumor, which can help predict the therapeutic response.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 3","pages":"1189-1204"},"PeriodicalIF":3.2,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13064617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147670298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continuous scanning full-field OCT for fast volumetric imaging of multi-cellular aggregates. 连续扫描全视野OCT用于多细胞聚集体的快速体积成像。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-02-04 eCollection Date: 2026-03-01 DOI: 10.1364/BOE.578177

C Kersuzan, A Jana, A Badon

引用次数: 0

Reliability of diffuse optical spectroscopies for evaluation of cerebral hemometabolic stress in children with sickle cell anemia. 漫射光谱评估镰状细胞性贫血患儿脑血代谢应激的可靠性。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-02-04 eCollection Date: 2026-03-01 DOI: 10.1364/BOE.582416

Giovani G Martins, Rowan O Brothers, Kristin Wubbena, Mariam Akbar, Michael Arrington, Katherine B Turrentine, Sydney Triplett, Hongting Zhao, Tara M Urner, Shasha Bai, Amy Tang, Kirshma Khemani, Beatrice E Gee, R Clark Brown, Erin M Buckley

引用次数: 0

Fusing photoacoustic microscopy and ultrasound imaging: an artificial intelligence strategy for vessel enhancement in interventional guidance. 融合光声显微镜和超声成像：介入引导中血管增强的人工智能策略。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-02-04 eCollection Date: 2026-03-01 DOI: 10.1364/BOE.588654

Wenjie Yin, Yanping He, Yuxiang Chen, Zixi Liu, Jinjun Fang, Chaohao Liang, Zhen Yuan, Kun Qian, Wuyu Zhang, Jian Zhang

{"title":"Fusing photoacoustic microscopy and ultrasound imaging: an artificial intelligence strategy for vessel enhancement in interventional guidance.","authors":"Wenjie Yin, Yanping He, Yuxiang Chen, Zixi Liu, Jinjun Fang, Chaohao Liang, Zhen Yuan, Kun Qian, Wuyu Zhang, Jian Zhang","doi":"10.1364/BOE.588654","DOIUrl":"https://doi.org/10.1364/BOE.588654","url":null,"abstract":"Vessel localization is a key component of ultrasound (US) -guided interventional procedures. However, US imaging faces challenges such as insufficient resolution and a low recognition rate in skin vessel detection. In this study, a photoacoustic (PA) -US multi-modal imaging enhancement framework is proposed. High-resolution vascular light absorption maps from photoacoustic microscopy (PAM) provide objective and accurate labels for US images. Accurate enhancement of blood vessels in skin US images is realized by neural networks driven by high-quality data sources. The results of our novel architecture (UIU-Net) on an ex vivo dataset of controllable vascular complexity show that UIU-Net outperforms existing methods in complex vascular morphology. Based on in vivo experiments, UIU-Net predicts vessels with substantial similarity to actual vessels with the best performance compared to conventional methods, with a 25.57% improvement in the similarity coefficient. Extended to the rabbit ear vein puncture scenario, UIU-Net consistently enhances US images of deep microvasculature. This method successfully guided puncture interventions, establishing a US vascular enhancement paradigm guided by PA imaging. It provides an intelligent solution that combines anatomical fidelity with the ability to avoid microvessels, thereby reducing complications in minimally invasive interventional US settings.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 3","pages":"1173-1188"},"PeriodicalIF":3.2,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13064631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147670020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative holographic agglutination assay for immunoglobulin A. 免疫球蛋白A的定量全息凝集试验。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-02-03 eCollection Date: 2026-03-01 DOI: 10.1364/BOE.581211

Rushna Quddus, Kent Kirshenbaum, David G Grier

{"title":"Quantitative holographic agglutination assay for immunoglobulin A.","authors":"Rushna Quddus, Kent Kirshenbaum, David G Grier","doi":"10.1364/BOE.581211","DOIUrl":"https://doi.org/10.1364/BOE.581211","url":null,"abstract":"This study introduces a holographic agglutination assay for quantifying levels of the immunoglobulin protein IgA in biological samples. This is the first example of a label-free and bead-free assay that quantifies protein agglutinates by direct detection using Total Holographic Characterization. A proof-of-concept assay for human serum immunoglobulins is demonstrated using Jacalin, the galactose-specific plant lectin, to induce selective agglutination. By analyzing the size, refractive index, and number of particles in an assay sample, we obtain a reproducible and quantitative measurement of galactosylated immunoglobulins in a given sample. The assay is calibrated for a reference interval of IgA concentrations from 4 mg dL-1 (0.25 μM) to 32 mg dL-1 (2 μM) with a limit of detection (LOD) of 6.3 mg dL-1 (0.39 μM) in emulated biological samples. This covers the physiologically relevant concentration range with ten-fold dilution. The assay clearly distinguishes samples containing IgA from samples containing IgG. More broadly, this study introduces a platform for creating lectin-mediated holographic agglutination assays to monitor levels of immunoglobulins in biological samples. The ability to quantify immunoglobulin levels efficiently in clinical samples is likely to be valuable for diagnostics and will provide a basis for assaying other proteins that can be induced to agglutinate.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 3","pages":"1125-1137"},"PeriodicalIF":3.2,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13064609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147670193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

fNIRS reveals comparable reductions in cortical hyperconnectivity following once-daily and twice-daily rTMS in poststroke aphasia. fNIRS显示，卒中后失语症患者在每日1次和每日2次rTMS治疗后，皮质超连通性明显降低。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-02-03 eCollection Date: 2026-03-01 DOI: 10.1364/BOE.583648

Chong Lu, Yizhen Liu, Yao Wang, Tiantian Xue, Siyu Wang, Tianxu Chen

{"title":"fNIRS reveals comparable reductions in cortical hyperconnectivity following once-daily and twice-daily rTMS in poststroke aphasia.","authors":"Chong Lu, Yizhen Liu, Yao Wang, Tiantian Xue, Siyu Wang, Tianxu Chen","doi":"10.1364/BOE.583648","DOIUrl":"https://doi.org/10.1364/BOE.583648","url":null,"abstract":"In clinical practice, there is a common assumption that more frequent intervention yields better outcomes for patients with post-stroke aphasia (PSA). This study investigated this by comparing the efficacy of once-daily versus twice-daily low-frequency repetitive transcranial magnetic stimulation (rTMS). In this non-randomized trial, 40 PSA patients self-selected into once-daily (n = 20) or twice-daily (n = 20) 1 Hz rTMS groups targeting the right Broca's area for three weeks, alongside 26 healthy controls. Functional near-infrared spectroscopy (fNIRS) assessed oxygenated hemoglobin-based functional connectivity (FC) during a verbal fluency task pre- and post-intervention. Data were analyzed using repeated-measures ANOVA. Pre-intervention, PSA patients exhibited maladaptive cortical hyperconnectivity. Following rTMS, both groups showed comparable improvement in aphasia quotient scores, with no significant between-group differences. Analysis of fNIRS data showed that both once-daily and twice-daily rTMS regimens led to a clear reduction in the count of strong FC edges, accompanied by an overall decrease in global mean FC following intervention. Notably, reductions in global mean FC were more pronounced in the twice-daily group, whereas only modest changes were observed following once-daily stimulation. Overall, both low-frequency rTMS regimens effectively attenuated maladaptive cortical hyperconnectivity in poststroke aphasia, supporting their role in network normalization. Importantly, the absence of a clear advantage for twice-daily stimulation challenges the \"more is better\" paradigm and highlights the potential value of individualized and cost-effective treatment strategies.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 3","pages":"1138-1151"},"PeriodicalIF":3.2,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13064620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147669693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0