Biomedical optics express最新文献

Improvements on speed, stability and field of view in adaptive optics OCT for anterior retinal imaging using a pyramid wavefront sensor. 利用金字塔波前传感器改进用于视网膜前部成像的自适应光学 OCT 的速度、稳定性和视野。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2024-09-30 eCollection Date: 2024-10-01 DOI: 10.1364/BOE.533451

Elisabeth Brunner, Laura Kunze, Victoria Laidlaw, Daniel Jodlbauer, Wolfgang Drexler, Ronny Ramlau, Andreas Pollreisz, Michael Pircher

{"title":"Improvements on speed, stability and field of view in adaptive optics OCT for anterior retinal imaging using a pyramid wavefront sensor.","authors":"Elisabeth Brunner, Laura Kunze, Victoria Laidlaw, Daniel Jodlbauer, Wolfgang Drexler, Ronny Ramlau, Andreas Pollreisz, Michael Pircher","doi":"10.1364/BOE.533451","DOIUrl":"https://doi.org/10.1364/BOE.533451","url":null,"abstract":"We present improvements on the adaptive optics (AO) correction method using a pyramid wavefront sensor (P-WFS) and introduce a novel approach for closed-loop focus shifting in retinal imaging. The method's efficacy is validated through in vivo adaptive optics optical coherence tomography (AO-OCT) imaging in both, healthy individuals and patients with diabetic retinopathy. In both study groups, a stable focusing on the anterior retinal layers is achieved. We further report on an improvement in AO loop speed that can be used to expand the imaging area of AO-OCT in the slow scanning direction, largely independent of the eye's isoplanatic patch. Our representative AO-OCT data reveal microstructural details of the neurosensory retina such as vessel walls and microglia cells that are visualized in single volume data and over an extended field of view. The excellent performance of the P-WFS based AO-OCT imaging in patients suggests good clinical applicability of this technology.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11482182/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlating stroke risk with non-invasive cerebrovascular perfusion dynamics using a portable speckle contrast optical spectroscopy laser device. 使用便携式斑点对比光学光谱激光设备，将中风风险与非侵入性脑血管灌注动态相关联。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2024-09-30 eCollection Date: 2024-10-01 DOI: 10.1364/BOE.534796

Yu Xi Huang, Simon Mahler, Aidin Abedi, Julian Michael Tyszka, Yu Tung Lo, Patrick D Lyden, Jonathan Russin, Charles Liu, Changhuei Yang

{"title":"Correlating stroke risk with non-invasive cerebrovascular perfusion dynamics using a portable speckle contrast optical spectroscopy laser device.","authors":"Yu Xi Huang, Simon Mahler, Aidin Abedi, Julian Michael Tyszka, Yu Tung Lo, Patrick D Lyden, Jonathan Russin, Charles Liu, Changhuei Yang","doi":"10.1364/BOE.534796","DOIUrl":"10.1364/BOE.534796","url":null,"abstract":"Stroke poses a significant global health threat, with millions affected annually, leading to substantial morbidity and mortality. Current stroke risk assessment for the general population relies on markers such as demographics, blood tests, and comorbidities. A minimally invasive, clinically scalable, and cost-effective way to directly measure cerebral blood flow presents an opportunity. This opportunity has the potential to positively impact effective stroke risk assessment prevention and intervention. Physiological changes in the cerebrovascular system, particularly in response to hypercapnia and hypoxia during voluntary breath-holding can offer insights into stroke risk assessment. However, existing methods for measuring cerebral perfusion reserves, such as blood flow and blood volume changes, are limited by either invasiveness or impracticality. Herein we propose a non-invasive transcranial approach using speckle contrast optical spectroscopy (SCOS) to non-invasively monitor regional changes in brain blood flow and volume during breath-holding. Our study, conducted on 50 individuals classified into two groups (low-risk and higher-risk for stroke), shows significant differences in blood dynamic changes during breath-holding between the two groups, providing physiological insights for stroke risk assessment using a non-invasive quantification paradigm. Given its cost-effectiveness, scalability, portability, and simplicity, this laser-centric tool has significant potential for early diagnosis and treatment of stroke in the general population.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11482158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photodynamic opening of the blood-brain barrier affects meningeal lymphatics and the brain's drainage in healthy male mice. 光动力打开血脑屏障会影响健康雄性小鼠的脑膜淋巴管和大脑引流。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2024-09-26 eCollection Date: 2024-10-01 DOI: 10.1364/BOE.527892

Inna Blokhina, Andrey Terskov, Arina Evsiukova, Alexander Dubrovsky, Viktoria Adushkina, Daria Zlatogorskaya, Alexander Dmitrenko, Matvey Tuzhilkin, Maria Manzhaeva, Valeria Krupnova, Egor Ilyukov, Dmitry Myagkov, Dmitry Tuktarov, Sergey Popov, Maria Tzoy, Alexander Shirokov, Ivan Fedosov, Oxana Semyachkina-Glushkovskaya

引用次数: 0

Stand-alone segmentation of blood flow pulsatility measured with diffuse correlation spectroscopy. 利用弥散相关光谱学测量血流脉动性的独立分割。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2024-09-26 eCollection Date: 2024-10-01 DOI: 10.1364/BOE.533916

Srinidhi Bharadwaj, Tara M Urner, Kyle R Cowdrick, Rowan O Brothers, Tisha Boodooram, Hongting Zhao, Vidisha Goyal, Eashani Sathialingam, Yueh-Chi Wu, Ayesha Quadri, Katherine Turrentine, Mariam M Akbar, Sydney E Triplett, Shasha Bai, Erin M Buckley

引用次数: 0

Light-adapted flicker-optoretinography based on raster-scan optical coherence tomography towards clinical translation. 基于光栅扫描光学相干断层扫描的光适配闪烁视网膜成像技术走向临床转化。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2024-09-26 eCollection Date: 2024-10-01 DOI: 10.1364/BOE.538481

Zhaoyu Gong, Yaping Shi, Jian Liu, Ramkumar Sabesan, Ruikang K Wang

{"title":"Light-adapted flicker-optoretinography based on raster-scan optical coherence tomography towards clinical translation.","authors":"Zhaoyu Gong, Yaping Shi, Jian Liu, Ramkumar Sabesan, Ruikang K Wang","doi":"10.1364/BOE.538481","DOIUrl":"https://doi.org/10.1364/BOE.538481","url":null,"abstract":"Optoretinography (ORG) is a promising non-invasive and objective technique for assessing retinal function by measuring its response to light stimulation. Optical coherence tomography (OCT) has emerged as a promising tool for implementing ORG due to its three-dimensional imaging capabilities, high sensitivity to nanometer-scale changes induced by light stimulation, and clinical availability. Although ORG has proven feasible in laboratory settings, research-grade OCT systems lack satisfactory usability and cost-effectiveness to be clinically viable. Standard clinical raster-scan OCT systems, with their limited imaging speed, fall short of the requirements for measuring rapid ORG responses. To bridge this gap, we introduce a flicker-ORG modality based on a raster-scan OCT system that resembles standard clinical OCT. This system overcomes speed limitations through an innovative two-stage scanning protocol coupled with a 600 kHz swept source, enabling repeated volume imaging and precise retinal activity measurements over a finite area. Additionally, the light-adapted ORG strategy eliminates the need for dark adaptation, allowing examinations under photopic conditions and thus improving patient compliance. We tested this new ORG method by measuring flicker-induced photoreceptor responses in five healthy subjects. The results demonstrated high repeatability and revealed dependencies of the ORG response on flicker frequency and retinal eccentricity. These findings, combined with the system's utility, cost-effectiveness, and ease of integration into existing technologies, underscore its substantial potential for clinical application.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11482172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stimulated Brillouin scattering flow cytometry. 受激布里渊散射流式细胞仪。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2024-09-25 eCollection Date: 2024-10-01 DOI: 10.1364/BOE.537602

Jake R Rosvold, Giulia Zanini, Chenchen Handler, Eric Frank, Jiarui Li, Michele I Vitolo, Stuart S Martin, Giuliano Scarcelli

引用次数: 0

OCTA-ReVA: an open-source toolbox for comprehensive retinal vessel feature analysis in optical coherence tomography angiography. OCTA-ReVA：用于光学相干断层血管造影中视网膜血管特征综合分析的开源工具箱。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2024-09-25 eCollection Date: 2024-10-01 DOI: 10.1364/BOE.537727

Albert K Dadzie, David Le, Mansour Abtahi, Behrouz Ebrahimi, Tobiloba Adejumo, Taeyoon Son, Michael J Heiferman, Jennifer I Lim, Xincheng Yao

{"title":"OCTA-ReVA: an open-source toolbox for comprehensive retinal vessel feature analysis in optical coherence tomography angiography.","authors":"Albert K Dadzie, David Le, Mansour Abtahi, Behrouz Ebrahimi, Tobiloba Adejumo, Taeyoon Son, Michael J Heiferman, Jennifer I Lim, Xincheng Yao","doi":"10.1364/BOE.537727","DOIUrl":"https://doi.org/10.1364/BOE.537727","url":null,"abstract":"Optical coherence tomography angiography (OCTA) has significantly advanced the study and diagnosis of eye diseases. However, current clinical OCTA systems and software tools lack comprehensive quantitative analysis capabilities, limiting their full clinical utility. This paper introduces the OCTA Retinal Vessel Analyzer (OCTA-ReVA), a versatile open-source platform featuring a user-friendly graphical interface designed for the automated extraction and quantitative analysis of OCTA features. OCTA-ReVA includes traditional established OCTA features based on binary vascular image processing, such as blood vessel density (BVD), foveal avascular zone area (FAZ-A), blood vessel tortuosity (BVT), and blood vessel caliber (BVC). Additionally, it introduces new features based on blood perfusion intensity processing, such as perfusion intensity density (PID), vessel area flux (VAF), and normalized blood flow index (NBFI), which provide deeper insights into retinal perfusion conditions. These additional capabilities are crucial for the early detection and monitoring of retinal diseases. OCTA-ReVA demystifies the intricate task of retinal vasculature quantification, offering a robust tool for researchers and clinicians to objectively evaluate eye diseases and enhance the precision of retinal health assessments.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11482184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-shot quantitative phase microscopy: a multi-functional tool for cell analysis. 单次定量相显微镜：细胞分析的多功能工具。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2024-09-24 eCollection Date: 2024-10-01 DOI: 10.1364/BOE.533091

Ana Espinosa-Momox, Brandon Norton, Maria Cywinska, Bryce Evans, Juan Vivero-Escoto, Rosario Porras-Aguilar

引用次数: 0

Development of a carboxymethyl chitosan functionalized slide for small molecule detection using oblique-incidence reflectivity difference technology. 利用斜入射反射率差技术开发用于小分子检测的羧甲基壳聚糖功能化载玻片。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2024-09-19 eCollection Date: 2024-10-01 DOI: 10.1364/BOE.534563

Mengjing Xu, Boyang Shi, Haofeng Li, Xiaohan Mai, Lan Mi, Jiong Ma, Xiangdong Zhu, Guowei Wang, Yiyan Fei

{"title":"Development of a carboxymethyl chitosan functionalized slide for small molecule detection using oblique-incidence reflectivity difference technology.","authors":"Mengjing Xu, Boyang Shi, Haofeng Li, Xiaohan Mai, Lan Mi, Jiong Ma, Xiangdong Zhu, Guowei Wang, Yiyan Fei","doi":"10.1364/BOE.534563","DOIUrl":"https://doi.org/10.1364/BOE.534563","url":null,"abstract":"Label-free optical biosensors have become powerful tools in the study of biomolecular interactions without the need for labels. High throughput and low detection limit are desirable for rapid and accurate biomolecule detection. The oblique-incidence reflectivity difference (OI-RD) technique is capable of detecting thousands of biomolecular interactions in a high-throughput mode, specifically for biomolecules larger than 1000 Da. In order to enhance the detection capability of OI-RD for small molecules (typically < 500 Da), we have developed a three-dimensional biochip that utilized carboxymethyl chitosan (CMCS) functionalized slides. By investigating various factors such as sonication time, protein immobilization time, CMCS molecular weight, and glutaraldehyde (GA) functionalization time, we have achieved a detection limit of 6.8 pM for avidin (68 kDa). Furthermore, accurate detection of D-biotin with a molecular weight of 244 Da has also been achieved. This paper presents an effective solution for achieving both high throughput and low detection limits using the OI-RD technique in the field of biomolecular interaction detection.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11482164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DMD and microlens array as a switchable module for illumination angle scanning in optical diffraction tomography. 将 DMD 和微透镜阵列作为光学衍射断层扫描中照明角扫描的可切换模块。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2024-09-18 eCollection Date: 2024-10-01 DOI: 10.1364/BOE.535123

Siqi Yang, Jeongsoo Kim, Mary E Swartz, Johann K Eberhart, Shwetadwip Chowdhury

{"title":"DMD and microlens array as a switchable module for illumination angle scanning in optical diffraction tomography.","authors":"Siqi Yang, Jeongsoo Kim, Mary E Swartz, Johann K Eberhart, Shwetadwip Chowdhury","doi":"10.1364/BOE.535123","DOIUrl":"10.1364/BOE.535123","url":null,"abstract":"Optical diffraction tomography (ODT) enables label-free and morphological 3D imaging of biological samples using refractive-index (RI) contrast. To accomplish this, ODT systems typically capture multiple angular-specific scattering measurements, which are used to computationally reconstruct a sample's 3D RI. Standard ODT systems employ scanning mirrors to generate angular illuminations. However, scanning mirrors are limited to illuminating the sample from only one angle at a time. Furthermore, when operated at high speeds, these mirrors may exhibit mechanical instabilities that compromise image quality and measurement speed. Recently, newer ODT systems have been introduced that utilize digital-micromirror devices (DMD), spatial light modulators (SLMs), or LED arrays to achieve switchable angle-scanning with no physically-scanning components. However, these systems associate with power inefficiencies and/or spurious diffraction orders that can also limit imaging performance. In this work, we developed a novel non-interferometric ODT system that utilizes a fully switchable module for angle scanning composed of a DMD and microlens array (MLA). Compared to other switchable ODT systems, this module enables each illumination angle to be generated fully independently from every other illumination angle (i.e., no spurious diffraction orders) while also optimizing the power efficiency based on the required density of illumination angles. We validate the quantitative imaging capability of this system using calibration microspheres. We also demonstrate its capability for imaging multiple-scattering samples by imaging an early-stage zebrafish embryo.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11482169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0