Biomedical optics express最新文献_第10页

Deep ultraviolet ultrashort laser pulses for precise ablation of soft biological tissue. 用于软组织精确消融的深紫外超短激光脉冲。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2025-12-10 eCollection Date: 2026-01-01 DOI: 10.1364/BOE.578629

Tatiana K Malikova, Rainer J Beck, Timothy P Frazer, Paul M Brennan, Kevin Dhaliwal, Robert R Thomson, Jonathan D Shephard

{"title":"Deep ultraviolet ultrashort laser pulses for precise ablation of soft biological tissue.","authors":"Tatiana K Malikova, Rainer J Beck, Timothy P Frazer, Paul M Brennan, Kevin Dhaliwal, Robert R Thomson, Jonathan D Shephard","doi":"10.1364/BOE.578629","DOIUrl":"10.1364/BOE.578629","url":null,"abstract":"Laser ablation offers the potential for precisely removing pathological tissue without damaging surrounding healthy structures. Among the existing laser types, deep ultraviolet ultrashort pulsed lasers offer the highest axial precision and reduced collateral damage, yet their application for ablating soft tissues apart from the cornea remains underexplored. Here, ablation of ex vivo lamb liver using laser pulses at 206 nm wavelength and 250 fs pulse duration is investigated. Laser parameters that enable clean, controlled tissue removal are identified by systematically varying the laser pulse energy, spot size, and pulse repetition rate, and the ablated tissues are analysed using histological analysis and surface profilometry. With optimised settings, tissue removal with axial precision down to 10 microns is demonstrated. Ablation threshold fluence of 38.7 ± 2.1 mJ·cm-2 is determined for lamb liver tissue, and fluence windows yielding precise ablation with no observable collateral damage are defined for different laser spot sizes. The ablation responses of tissues with different physical properties are also investigated. These results advance understanding of laser-tissue interaction in the deep ultraviolet ultrashort pulse regime and demonstrate the potential of the proposed tissue removal method for high-precision surgical applications.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 1","pages":"183-201"},"PeriodicalIF":3.2,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145965097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stiles-Crawford effect in infrared two-photon vision. 红外双光子视觉中的Stiles-Crawford效应。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2025-12-10 eCollection Date: 2026-01-01 DOI: 10.1364/BOE.581133

Xinyu Wang, Silvestre Manzanera, Juan Tabernero, Pablo Artal

引用次数: 0

Sub-50-femtosecond gain-managed amplified pulses enhance nonlinear ablation efficiency. 50飞秒以下的增益管理放大脉冲提高了非线性烧蚀效率。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2025-12-10 eCollection Date: 2026-01-01 DOI: 10.1364/BOE.582102

Liam J Price, Kai Zhang, Nicholas J Otero, Paul Repgen, Pablo A Valdes, Frank Wise, Fatih Ömer Ilday, Bryan Q Spring

{"title":"Sub-50-femtosecond gain-managed amplified pulses enhance nonlinear ablation efficiency.","authors":"Liam J Price, Kai Zhang, Nicholas J Otero, Paul Repgen, Pablo A Valdes, Frank Wise, Fatih Ömer Ilday, Bryan Q Spring","doi":"10.1364/BOE.582102","DOIUrl":"10.1364/BOE.582102","url":null,"abstract":"Nonlinear femtosecond (fs) laser ablation enables highly localized energy deposition for cell microsurgery. Conventional systems operate at either low (∼1 kHz, amplified µJ pulse energy) or high (∼80 MHz, unamplified low nJ) repetition rates, but intermediate rates with amplified pulse energy offer a promising balance of ablation speed and thermal control. We custom-built a low-cost, 32 MHz femtosecond fiber laser system with gain-managed nonlinear amplification, boosting pulse energy from 5 to 90 nJ while compressing pulse duration to 46 fs. In this intermediate-repetition-rate regime, the use of sub-50-fs pulses enhances ablation efficiency by strengthening multiphoton absorption and lowering the effective ablation threshold, while also leveraging multi-pulse incubation effects that promote cumulative energy deposition at reduced per-pulse energies. Compared to 200-500 fs pulses typically used for ablation, shorter durations double ablation efficiency in silicon and yield a ∼10× increase in cell membrane damage. In 3D tumor models, this approach enables targeted subsurface ablation up to 400 µm depth with 6 nJ pulse energy. These results inform the design of next-generation femtosecond laser systems for microsurgery.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 1","pages":"202-216"},"PeriodicalIF":3.2,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145965291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical characterization of ocular collagen microstructure and anisotropy in animal and human eyes. 动物和人眼眼胶原结构和各向异性的光学表征。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2025-12-09 eCollection Date: 2026-01-01 DOI: 10.1364/BOE.581592

Zhenli Lin, Haiyang Li, Zhi Zeng, Yuxi Wang, Rui Hu, Junle Qu, Liwei Liu, Binglin Shen

引用次数: 0

High-throughput spontaneous Raman imaging of biological specimens with water background reduction. 水背景还原生物标本的高通量自发拉曼成像。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2025-12-08 eCollection Date: 2026-01-01 DOI: 10.1364/BOE.578769

Tomoaki Okumura, Yasuaki Kumamoto, Nicholas Isaac Smith, Katsumasa Fujita

引用次数: 0

Diffusion prior-based zero-shot denoising for real-world fluorescence microscopy images via encoder-decoder guided sampling. 基于扩散先验的基于编码器-解码器引导采样的真实荧光显微镜图像零采样去噪。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2025-12-08 eCollection Date: 2026-01-01 DOI: 10.1364/BOE.578464

Qinxuan Luo, Ziwen Liu, Ge Yang

{"title":"Diffusion prior-based zero-shot denoising for real-world fluorescence microscopy images via encoder-decoder guided sampling.","authors":"Qinxuan Luo, Ziwen Liu, Ge Yang","doi":"10.1364/BOE.578464","DOIUrl":"10.1364/BOE.578464","url":null,"abstract":"Fluorescence microscopy is an important imaging technique for biological research and applications. However, owing to various constraints in its engineering implementation and image acquisition, the acquired images often suffer from substantial noise. Although many denoising methods have been proposed, their applicability is limited by the distinct properties of fluorescence microscopy images. In particular, in real-world applications, it is often challenging and sometimes infeasible to acquire a large number of paired noisy-clean images for supervised training, and it is impractical to parameterize and estimate all types of real-world noise distributions. We propose ED-Diff, a zero-shot denoising algorithm based on diffusion priors. ED-Diff integrates the optimization solution of inverse problems with the diffusion sampling process and introduces a noisy image transformation module (NiTM) with an encoder-decoder structure to handle real-world noise scenarios. Extensive experiments on multiple real-world datasets validated the effectiveness of NiTM, demonstrating that ED-Diff exhibits competitive and robust performance.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 1","pages":"145-159"},"PeriodicalIF":3.2,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145965032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptive optics stimulated Raman scattering microscopy for topical product pharmacokinetic imaging. 局部产品药代动力学成像的自适应光学刺激拉曼散射显微镜。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2025-12-08 eCollection Date: 2026-01-01 DOI: 10.1364/BOE.576409

Saara K Luna, Benjamin A Kuzma, Nick-Sidney Lemberger, Kristin Wallmeier, Carsten Fallnich, Conor L Evans

{"title":"Adaptive optics stimulated Raman scattering microscopy for topical product pharmacokinetic imaging.","authors":"Saara K Luna, Benjamin A Kuzma, Nick-Sidney Lemberger, Kristin Wallmeier, Carsten Fallnich, Conor L Evans","doi":"10.1364/BOE.576409","DOIUrl":"10.1364/BOE.576409","url":null,"abstract":"Topically applied drug products are used to treat various dermatological conditions, enabling local and targeted delivery of active pharmaceutical ingredients. To ensure appropriate topical drug efficacy and delivery, it is necessary to understand the pharmacokinetics of topical drug uptake. Stimulated Raman scattering (SRS) microscopy, a chemically specific imaging modality, is well-suited to quantitatively measure the spatiotemporal kinetics of topical drug permeation in skin. However, SRS imaging depth in skin is often limited due to skin heterogeneity and scattering effects, distorting the laser focus and reducing the SRS signal. Adaptive optics (AO) can help overcome and correct depth limitations by adjusting wavefront shape and improving laser focus with a programmable optical element, such as a deformable membrane mirror. Previous work has demonstrated the use of AO in optimizing coherent anti-Stokes Raman scattering (CARS) signal in tissue imaging. In this study, we evaluated the ability of AO to improve the SRS signal in tissue imaging using a deformable membrane mirror as the AO element, which utilized a simulated annealing algorithm to search for mirror shapes producing optimal SRS signal at different tissue imaging depths. AO SRS images had an 11% increase in SRS signal intensity and >350% increase in image contrast between high and low signal regions compared to non-AO SRS images in nude mouse ear skin at the same depth. Mirror shapes optimized at greater imaging depths produced increased SRS intensity and contrast for a range of imaging depths (up to 290 μm in chicken muscle). Using AO SRS, we performed time-lapse imaging of ruxolitinib uptake in ex vivo human skin, demonstrating that AO SRS can be used to quantitatively measure topical drug pharmacokinetics in deeper skin structures (>100 μm).","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 1","pages":"125-144"},"PeriodicalIF":3.2,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145965082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hybrid particle-wave Monte Carlo OCT simulation method provides a three orders of magnitude improvement in efficiency. 混合粒子波蒙特卡罗OCT模拟方法的效率提高了三个数量级。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2025-12-04 eCollection Date: 2026-01-01 DOI: 10.1364/BOE.572256

Gijs Buist, Arjen Amelink, Johannes F de Boer

{"title":"Hybrid particle-wave Monte Carlo OCT simulation method provides a three orders of magnitude improvement in efficiency.","authors":"Gijs Buist, Arjen Amelink, Johannes F de Boer","doi":"10.1364/BOE.572256","DOIUrl":"10.1364/BOE.572256","url":null,"abstract":"The attenuation coefficient of biological tissue could serve as an indicator of structural and functional changes related to the onset or progression of disease. Optical coherence tomography (OCT) provides cross-sectional images of tissue up to a depth of a few millimeters, based on the local backscatter properties. Monte Carlo (MC) simulations are ideally suited to investigate and improve OCT attenuation coefficient extraction in confounding cases of (low-order) multiple scattering and inclusions such as blood vessels. However, current MC methods are time-consuming due to the OCT detection configuration rejecting many of the backscattered photons. In this work, we present two MC OCT detection models, a conventional photon detection model and a hybrid particle-wave detection model based on spherical waves from a photon's last scatter position, and compare their simulation efficiency by comparing the SNR of the resulting depth profiles for equal conditions and number of simulated photons. We find a three orders of magnitude increase in the simulation efficiency for the hybrid particle-wave model compared to the conventional photon detection model. Both models show excellent agreement with single-scatter theory for weakly scattering samples. Additionally, we use the hybrid particle-wave model to simulate experimentally measured samples with 0.1 mm-1 ≤µ s ≤ ~4.5 mm-1. The resulting simulated depth profiles show excellent agreement with the experimental depth profiles, even in those cases where the single-scatter theory model fails to describe the experimental depth profiles accurately.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 1","pages":"49-67"},"PeriodicalIF":3.2,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145965126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Translational photoacoustic and ultrasound imaging catheter for characterizing gastrointestinal inflammation and fibrosis: a study with a pig model in vivo. 翻译光声和超声成像导管表征胃肠道炎症和纤维化：猪体内模型的研究。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2025-12-04 eCollection Date: 2026-01-01 DOI: 10.1364/BOE.577556

Yaocai Huang, Laura A Johnson, Xiaorui Peng, Yao Lee, Xueding Wang, Peter D R Higgins, Guan Xu

{"title":"Translational photoacoustic and ultrasound imaging catheter for characterizing gastrointestinal inflammation and fibrosis: a study with a pig model in vivo.","authors":"Yaocai Huang, Laura A Johnson, Xiaorui Peng, Yao Lee, Xueding Wang, Peter D R Higgins, Guan Xu","doi":"10.1364/BOE.577556","DOIUrl":"10.1364/BOE.577556","url":null,"abstract":"Our previous studies demonstrated that a prototype photoacoustic (PA) and ultrasound (US) imaging catheter can differentiate intestinal inflammation and fibrosis. However, its compatibility with clinical endoscopic procedures has not been validated. Here, we present a translational PA-US dual-modality imaging catheter with a reduced diameter to fit the biopsy channel of standard adult colonoscopes and therapeutic upper endoscopes (∼3.7 mm). The catheter integrates an angle-tipped optical fiber (600-µm core) for PA illumination and a miniaturized 48-element ultrasound array operating at a center frequency of ∼9 MHz for signal reception. These components are enclosed in a hydrostatic balloon (12 mm diameter, 15 mm length when fully inflated) to ensure acoustic coupling with the intestinal lumen. The system was upgraded from our previous setup by incorporating a more portable optical parametric oscillator (OPO) laser and a Verasonics imaging platform. The imaging catheter was positioned at the disease locations in a pig model of esophageal fibrosis induced by argon plasma coagulation (APC). Multi-wavelength PA imaging and US imaging were performed to resolve the tissue components. The results demonstrate the catheter's ability to assess inflammation and fibrosis in the gastrointestinal tract during a standard clinical endoscopy procedure.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 1","pages":"98-108"},"PeriodicalIF":3.2,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145964708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-free viability assessment of ex vivo mouse kidneys for transplant applications using dynamic optical coherence tomography. 使用动态光学相干断层扫描对移植应用的小鼠离体肾脏进行无标记活力评估。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2025-12-04 eCollection Date: 2026-01-01 DOI: 10.1364/BOE.579100

Ke Zhang, Qinghao Zhang, Trisha Valerio, Feng Yan, Yan Cui, Ronghao Liu, Xiaoyu Ma, Junyuan Liu, Tri Vu, Chen Wang, Bornface Mutembei, Kaustubh Pandit, Fabricio Silveyra, Dan Duong, Clint Hostetler, Ashley Milam, Bradon Nave, Ron Squires, Zhongxin Yu, Kar-Ming Fung, Narendra Battula, Steven Potter, Chongle Pan, Wei R Chen, Yuchen Qiu, Javier Jo, Paulo Martins, Yuye Ling, Qinggong Tang

{"title":"Label-free viability assessment of ex vivo mouse kidneys for transplant applications using dynamic optical coherence tomography.","authors":"Ke Zhang, Qinghao Zhang, Trisha Valerio, Feng Yan, Yan Cui, Ronghao Liu, Xiaoyu Ma, Junyuan Liu, Tri Vu, Chen Wang, Bornface Mutembei, Kaustubh Pandit, Fabricio Silveyra, Dan Duong, Clint Hostetler, Ashley Milam, Bradon Nave, Ron Squires, Zhongxin Yu, Kar-Ming Fung, Narendra Battula, Steven Potter, Chongle Pan, Wei R Chen, Yuchen Qiu, Javier Jo, Paulo Martins, Yuye Ling, Qinggong Tang","doi":"10.1364/BOE.579100","DOIUrl":"10.1364/BOE.579100","url":null,"abstract":"Kidney transplantation remains the preferred treatment for patients with end-stage kidney disease. However, the ongoing shortage of donor organs continues to limit the availability of transplant treatments. Existing evaluation methods, such as the kidney donor profile index (KDPI) and pre-transplant donor biopsy (PTDB), have various limitations, including low discriminative power, invasiveness, and sampling errors, which reduce their effectiveness in organ quality assessment and contribute to the risk of unnecessary organ discard. In this study, we explored the dynamic optical coherence tomography (DOCT) as a label-free, non-invasive approach to monitor the viability of ex vivo mouse kidneys during static cold storage over 48 hours. The dynamic metrics logarithmic intensity variance (LIV), early OCT correlation decay speed (OCDS e ), and late OCT correlation decay speed (OCDS l ) were extracted from OCT signal fluctuations to quantify temporal and spatial tissue activity and deterioration. Our results demonstrate that DOCT provides complementary information relevant to tissue viability, in addition to the morphological assessment offered by conventional OCT imaging, showing potential to improve pre-transplant organ evaluation and clinic decision-making.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 1","pages":"68-81"},"PeriodicalIF":3.2,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145965177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0