Biomedical optics express最新文献_第9页

HDB-Net: hierarchical dual-branch network for retinal layer segmentation in diseased OCT images. HDB-Net：用于病变 OCT 图像视网膜层分割的分层双分支网络。

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-19 DOI: 10.1364/boe.530469

Yu Chen,XueHe Zhang,Jiahui Yang,Gang Han,He Zhang,MingZhu Lai,Jie Zhao

{"title":"HDB-Net: hierarchical dual-branch network for retinal layer segmentation in diseased OCT images.","authors":"Yu Chen,XueHe Zhang,Jiahui Yang,Gang Han,He Zhang,MingZhu Lai,Jie Zhao","doi":"10.1364/boe.530469","DOIUrl":"https://doi.org/10.1364/boe.530469","url":null,"abstract":"Optical coherence tomography (OCT) retinal layer segmentation is a critical procedure of the modern ophthalmic process, which can be used for diagnosis and treatment of diseases such as diabetic macular edema (DME) and multiple sclerosis (MS). Due to the difficulties of low OCT image quality, highly similar retinal interlayer morphology, and the uncertain presence, shape and size of lesions, the existing algorithms do not perform well. In this work, we design an HDB-Net network for retinal layer segmentation in diseased OCT images, which solves this problem by combining global and detailed features. First, the proposed network uses a Swin transformer and Res50 as a parallel backbone network, combined with the pyramid structure in UperNet, to extract global context and aggregate multi-scale information from images. Secondly, a feature aggregation module (FAM) is designed to extract global context information from the Swin transformer and local feature information from ResNet by introducing mixed attention mechanism. Finally, the boundary awareness and feature enhancement module (BA-FEM) is used to extract the retinal layer boundary information and topological order from the low-resolution features of the shallow layer. Our approach has been validated on two public datasets, and Dice scores were 87.61% and 92.44, respectively, both outperforming other state-of-the-art technologies.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"21 1","pages":"5359-5383"},"PeriodicalIF":3.4,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico full-angle high-dynamic range scattering of microscopic objects exploiting holotomography. 利用全息图对微观物体进行硅学全角度高动态范围散射。

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-15 DOI: 10.1364/boe.528698

Seung Kyu Kang,Kyoohyun Kim,Jinsoo Jeong,Sunghee Hong,YongKeun Park,Jonghwa Shin

{"title":"In silico full-angle high-dynamic range scattering of microscopic objects exploiting holotomography.","authors":"Seung Kyu Kang,Kyoohyun Kim,Jinsoo Jeong,Sunghee Hong,YongKeun Park,Jonghwa Shin","doi":"10.1364/boe.528698","DOIUrl":"https://doi.org/10.1364/boe.528698","url":null,"abstract":"Accurate optical characterization of microscopic objects is crucial in academic research, product development, and clinical diagnosis. We present a method for obtaining full and high-dynamic range, angle-resolved light scattering attributes of microparticles, enabling distinction of variations in both overall morphology and detailed internal structures. This method overcomes previous limitations in observable scattering angles and dynamic range of signals through computationally assisted three-dimensional holotomography. This advancement is significant for particles spanning tens of wavelengths, such as human erythrocytes, which have historically posed measurement challenges due to faint side-scattering signals indicative of their complex interiors. Our technique addresses three key challenges in optical side-scattering analysis: limited observational angular range, reliance on simplified computational models, and low signal-to-noise ratios in both experimental and computational evaluations. We incorporate three-dimensional tomographic complex refractive index data from Fourier-transform light scattering into a tailored finite-difference time-domain simulation space. This approach facilitates precise near-to-far-field transformations. The process yields complete full-angle scattering phase functions, crucial for particles like Plasmodium falciparum-parasitized erythrocytes, predominantly involved in forward scattering. The resultant scattering data exhibit an extreme dynamic range exceeding 100 dB at various incident angles of a He-Ne laser. These findings have the potential to develop point-of-care, cost-effective, and rapid malaria diagnostic tools, inspiring further clinical and research applications in microparticle scattering.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"14 1","pages":"5238-5250"},"PeriodicalIF":3.4,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variation of skin hydration profile with biophysical factors and lifestyle revealed by in vivo terahertz sensing. 活体太赫兹传感技术揭示皮肤水合状况随生物物理因素和生活方式的变化。

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-13 DOI: 10.1364/boe.527731

Xuefei Ding,A I Hernandez-Serrano,Jacob J Young,Emma Pickwell-MacPherson

{"title":"Variation of skin hydration profile with biophysical factors and lifestyle revealed by in vivo terahertz sensing.","authors":"Xuefei Ding,A I Hernandez-Serrano,Jacob J Young,Emma Pickwell-MacPherson","doi":"10.1364/boe.527731","DOIUrl":"https://doi.org/10.1364/boe.527731","url":null,"abstract":"The skin, being the body's largest organ, plays a pivotal role in protecting the body against dangerous external factors. The maintenance of adequate hydration levels is essential for the skin to fulfill this protective function. However, skin hydration depends upon different biophysical factors and lifestyles, such as ethnicity, sex, age, water consumption, and many more. Consequently, methods to assess skin hydration in a precise and non-invasive manner are in high demand. In this paper, using a portable and handheld terahertz (THz) probe, we systematically examine the correlation between diverse biophysical factors and skin hydration profile in a population exceeding 300 participants. Through comparative analysis of THz light reflected from the skin against a dielectric model, we successfully extracted the thickness and hydration percentage of the outermost layer of the epidermis, the stratum corneum (SC). Our findings indicate that SC hydration and thickness are associated with variables such as daily water consumption, age, drinking coffee, and exercise. Additionally, our measurements reveal distinctions in the skin's hydration properties concerning susceptibility to UV-induced effects by bringing in the Fitzpatrick skin types. This THz-based technique holds the potential for facile integration into clinical settings for the evaluation and diagnosis of various skin-related conditions.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"10 1","pages":"5180-5198"},"PeriodicalIF":3.4,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling enamel demineralization mechanisms by sensitive dielectric differentiation based on terahertz nanospectroscopy. 基于太赫兹纳米光谱学的灵敏介电分辨技术揭示釉质脱矿机制

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-13 DOI: 10.1364/boe.527554

Feng Xiao,Xiaoqiuyan Zhang,Xingxing Xu,Tianyu Zhang,Fu Tang,Haowei Yin,Tao Hu,Lei Lei,Li Cheng,Min Hu

{"title":"Unveiling enamel demineralization mechanisms by sensitive dielectric differentiation based on terahertz nanospectroscopy.","authors":"Feng Xiao,Xiaoqiuyan Zhang,Xingxing Xu,Tianyu Zhang,Fu Tang,Haowei Yin,Tao Hu,Lei Lei,Li Cheng,Min Hu","doi":"10.1364/boe.527554","DOIUrl":"https://doi.org/10.1364/boe.527554","url":null,"abstract":"The early stage of dental caries, i.e. demineralization, has always been a topic of concern to dentists. Understanding the essential mechanism of its occurrence is of great significance for the prevention and treatment of dental caries. However, owing to limitations in resolution and the detection capabilities of diagnostic tools, the study of enamel demineralization has always been a challenge. Terahertz (THz) technology, especially the combination of scanning near-field optical microscopy (s-SNOM) and THz time-domain spectroscopy (TDS), due to its nanoscale resolution, has shown great advantages in the field of biological imaging. Here, a THz s-SNOM system is used to perform near-field imaging of enamel before and after demineralization at the nanoscale. It can be found that near-field signals decrease significantly after demineralization. This is due to the changes of the crystal lattice and the transfer of mineral ions during demineralization, which leads to a decrease in the permittivity of the enamel. The novel approach in this study reveals the essence of demineralization and lays the groundwork for additional research and potential interventions.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"65 1","pages":"5229-5237"},"PeriodicalIF":3.4,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum: Towards improved 3D reconstruction of cystoscopies through real-time feedback for frame reacquisition: erratum. 勘误：通过帧再获取的实时反馈改进膀胱镜检查的三维重建：勘误。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2024-08-13 eCollection Date: 2024-09-01 DOI: 10.1364/BOE.538890

Rachel Eimen, Mayaank Pillai, Kristen R Scarpato, Audrey K Bowden

引用次数: 0

Comparison of vinculin tension in cellular monolayers and three-dimensional multicellular aggregates. 细胞单层和三维多细胞聚集体中长春花素张力的比较。

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-13 DOI: 10.1364/boe.529156

Luni Hu,Rick I Cohen,Margarida Barroso,Nada N Boustany

{"title":"Comparison of vinculin tension in cellular monolayers and three-dimensional multicellular aggregates.","authors":"Luni Hu,Rick I Cohen,Margarida Barroso,Nada N Boustany","doi":"10.1364/boe.529156","DOIUrl":"https://doi.org/10.1364/boe.529156","url":null,"abstract":"Confocal frequency-domain fluorescence lifetime and Förster resonance energy transfer (FRET) microscopy of Chinese hamster ovary (CHO-K1) cells expressing the vinculin tension sensor (VinTS) is used to compare vinculin tension in three-dimensional (3D) multicellular aggregates and 2D cellular monolayers. In both 2D and 3D cultures, the FRET efficiency of VinTS is 5-6% lower than that of VinTL (p < 0.05), a tail-less control which cannot bind actin or paxillin. The difference between VinTS and VinTL FRET efficiency can be mitigated by treatment with the Rho-associated kinase inhibitor Y-27632, demonstrating that VinTS is under tension in both 2D and 3D cultures. However, there is an overall decrease in FRET efficiency of both VinTS and VinTL in the 3D multicellular aggregates compared with the 2D monolayers. Expression of VinTS in 2D and 3D cultures exhibits puncta consistent with cellular adhesions. While paxillin is present at the sites of VinTS expression in the 2D monolayers, it is generally absent from VinTS puncta in the 3D aggregates. The results suggest that VinTS experiences a modified environment in 3D aggregates compared with 2D monolayers and provide a basis for further investigation of molecular tension sensors in 3D tissue models.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"14 1","pages":"5199-5214"},"PeriodicalIF":3.4,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid, sensitive and multiplexed detection of SARS-CoV-2 viral nucleic acids enabled by phase-based surface plasmon resonance of metallic gratings. 利用金属光栅的相位表面等离子体共振技术实现对 SARS-CoV-2 病毒核酸的快速、灵敏和多重检测。

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-13 DOI: 10.1364/boe.535051

Shuai Sun,Yuanyuan Zhao,Yuxuan Hao,Pan Xue,Xiaoyang Guo,Wei Zhang,Chunxiang Zhang,Qiongdi Zhang,Wenhua Zhou,Hock Chun Ong,Xuefeng Yu,Zeren Li,Jia Li,Jianquan Yao

引用次数: 0

SODL-IR-FISTA: sparse online dictionary learning with iterative reduction FISTA for cone-beam X-ray luminescence computed tomography. SODL-IR-FISTA：用于锥束 X 射线发光计算机断层扫描的稀疏在线词典学习与迭代还原 FISTA。

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-12 DOI: 10.1364/boe.531828

Xin Cao,Wenlong Tang,Huimin Gao,Yifan Wang,Yi Chen,Chengyi Gao,Fengjun Zhao,Linzhi Su

{"title":"SODL-IR-FISTA: sparse online dictionary learning with iterative reduction FISTA for cone-beam X-ray luminescence computed tomography.","authors":"Xin Cao,Wenlong Tang,Huimin Gao,Yifan Wang,Yi Chen,Chengyi Gao,Fengjun Zhao,Linzhi Su","doi":"10.1364/boe.531828","DOIUrl":"https://doi.org/10.1364/boe.531828","url":null,"abstract":"Cone beam X-ray luminescence computed tomography (CB-XLCT) is an emerging imaging technique with potential for early 3D tumor detection. However, the reconstruction challenge due to low light absorption and high scattering in tissues makes it a difficult inverse problem. In this study, the online dictionary learning (ODL) method, combined with iterative reduction FISTA (IR-FISTA), has been utilized to achieve high-quality reconstruction. Our method integrates IR-FISTA for efficient and accurate sparse coding, followed by an online stochastic approximation for dictionary updates, effectively capturing the sparse features inherent to the problem. Additionally, a re-sparse step is introduced to enhance the sparsity of the solution, making it better suited for CB-XLCT reconstruction. Numerical simulations and in vivo experiments were conducted to assess the performance of the method. The SODL-IR-FISTA achieved the smallest location error of 0.325 mm in in vivo experiments, which is 58% and 45% of the IVTCG-L 1 (0.562 mm) and OMP-L 0 (0.721 mm), respectively. Additionally, it has the highest DICE similarity coefficient, which is 0.748. The results demonstrate that our approach outperforms traditional methods in terms of localization precision, shape restoration, robustness, and practicality in live subjects.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"11 1","pages":"5162-5179"},"PeriodicalIF":3.4,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adversarial training collaborating hybrid convolution-transformer network for automatic identification of reactive lymphocytes in peripheral blood. 用于自动识别外周血中反应性淋巴细胞的对抗训练协作混合卷积-变换器网络。

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-09 DOI: 10.1364/boe.525119

Liye Mei,Haoran Peng,Ping Luo,Shuangtong Jin,Hui Shen,Jing He,Wei Yang,Zhiwei Ye,Haigang Sui,Mengqing Mei,Cheng Lei,Bei Xiong

引用次数: 0

Chromatic cues for the sign of defocus in the peripheral retina. 周边视网膜散焦迹象的色度线索。

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-08 DOI: 10.1364/boe.537268

Len Zheleznyak,Chang Liu,Simon Winter

引用次数: 0