Biomedical optics express最新文献

{"title":"Light-sheet microscopy enabled by a miniaturized plane illuminator.","authors":"Seonho Kim, Yeochan Yun, Hyeon-Yeol Cho, Sucbei Moon","doi":"10.1364/BOE.547464","DOIUrl":"10.1364/BOE.547464","url":null,"abstract":"<p><p>We present a implementation method of light-sheet microscopy utilizing a highly miniaturized device that produces light-sheet illumination while immersed in the sample container. Our miniaturized plane illuminator (MPI) internally equips a two-axis beam-scanning mechanism based on a magnetostatically driven optical fiber cantilever. A light sheet is produced by fast scanning of the focused beam in an axis while the illumination plane can move in the other axis for positioning and 3D imaging. Our MPI device is so compact in a 1.5 mm-thick needle form that it can be conveniently placed in the right vicinity of the imaging sample. Because the illumination is directly given in the sample-surrounding medium, a great deal of operational flexibility is obtained with an uncompromised beam quality. We could build a light-sheet microscopy system with a conventional inverted microscope frame by attaching our MPI upgrade kit as an add-on module. In this study, the optical and electromechanical characteristics of our MPI device were carefully investigated. As well, light-sheet microscopy imaging of various samples was performed to validate the practical power of our technique. We found our MPI can provide a low-cost and easy-to-use imaging mode, and make the light-sheet microscopy more available in various applications.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 1","pages":"115-125"},"PeriodicalIF":2.9,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum: Non-invasive glucose extraction by a single polarization rotator system in patients with diabetes: erratum.","authors":"Yu-Lung Lo, Yi-Sheng Chen, Po-Yu Wang, Ching-Min Chang, Guan-Ting Wei, Wei-Chun Hung","doi":"10.1364/BOE.550680","DOIUrl":"https://doi.org/10.1364/BOE.550680","url":null,"abstract":"<p><p>[This corrects the article on p. 4909 in vol. 15, PMID: 39346983.].</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 1","pages":"111-114"},"PeriodicalIF":2.9,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High dynamic range widefield fundus photography with transcranial illumination: a contrast agent-free method for non-mydriatic choroidal imaging.","authors":"Mojtaba Rahimi, Alfa Rossi, Taeyoon Son, Tobiloba Adejumo, Albert K Dadzie, Michael J Heiferman, Xincheng Yao","doi":"10.1364/BOE.543907","DOIUrl":"10.1364/BOE.543907","url":null,"abstract":"<p><p>The choroid, a critical vascular layer beneath the retina, is essential for maintaining retinal function and monitoring chorioretinal disorders. Existing imaging methods, such as indocyanine green angiography (ICGA) and optical coherence tomography (OCT), face significant limitations, including contrast agent requirements, restricted field of view (FOV), and high costs, limiting accessibility. To address these challenges, we developed a nonmydriatic, contrast agent-free fundus camera utilizing transcranial near-infrared (NIR) illumination. This system achieves a wide snapshot FOV of up to 185° eye-angle (130° visual-angle) without pharmacological pupillary dilation or contrast agents. By montaging two HDR images, the effective FOV can exceed 220° eye-angle (160° visual angle). Employing high dynamic range (HDR) imaging, the device ensures uniform contrast and enhanced choroidal visualization by correcting illumination inhomogeneity. The system demonstrated imaging performance comparable to ICGA when tested on healthy participants and patients with choroidal conditions, offering improved accessibility and affordability. This innovation holds promise for advancing the screening, diagnosis, and management of choroidal disorders, particularly in underserved settings.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 1","pages":"84-96"},"PeriodicalIF":2.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasmonic Ag/PMMA/Eu nanocomposite for sensitive dual mode detection of malachite green.","authors":"Linlin Tian, Ziheng Song, Chengmei Sun, Chengcheng Xu, Dong Zhang, Qingru Wang","doi":"10.1364/BOE.544257","DOIUrl":"10.1364/BOE.544257","url":null,"abstract":"<p><p>Accurate and efficient determination of malachite green (MG) in aquaculture is crucial for ensuring environment and food safety. Herein, we present a dual-response fluorescence probe based on an Ag/PMMA/Eu nanocomposite for the sensitive detection of MG with low concentration and single droplet. The luminescence properties of the Ag/PMMA/Eu nanocomposite and the fluorescence resonance energy transfer (FRET) effect between Eu and MG are significantly improved due to the localized surface plasmon resonance (LSPR) effect. The dual-response system enables the detection of MG through both luminescence intensity and energy transfer efficiency using the Ag/PMMA/Eu nanocomposite as a detection platform in the range of 0-10.78 µmol/L. The detection limit reaches as low as 0.5 nmol/L, a significant improvement over the 0.11 µmol/L limit achievable by pure Eu film alone, demonstrating superior sensitivity compared to traditional fluorescence detection techniques. The results indicate that the nanocomposite significantly boosts the sensitivity of the dual-mode sensors. In addition, the sensor successfully detects MG residues in lake water, highlighting the Ag/PMMA/Eu nanocomposite's potential to advance high sensitivity, selectivity, stability, and accurate detection in food security and biological analysis.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 1","pages":"97-110"},"PeriodicalIF":2.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Motion-less depth-selective optogenetic probe using tapered fiber and an electrically tuneable liquid crystal steering element.","authors":"William Boissonneault, Maxime Lemieux, Frédéric Bretzner, Tigran Galstian","doi":"10.1364/BOE.544388","DOIUrl":"https://doi.org/10.1364/BOE.544388","url":null,"abstract":"<p><p>A miniature electrically tuneable liquid crystal component is used to steer light from -1° to +1° and then to inject into a simple tapered fiber. This allows the generation of various propagation modes, their leakage, and selective illumination of the surrounding medium at different depth levels without using mechanical movements nor deformation. The performance of the device is characterized in a reference fluorescence medium (Rhodamine 6G) as well as in a mouse brain (medullary reticular formation and mesencephalic locomotor regions) during in-vivo experiments as a proof of concept. This device may be further miniaturized to be applied to freely behaving animals for the dynamic selective excitation or inhibition of different brain regions.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 1","pages":"68-83"},"PeriodicalIF":2.9,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optical diffraction tomography using a self-reference module.","authors":"Zhengyuan Tang, Julianna Winnik, Bryan M Hennelly","doi":"10.1364/BOE.545296","DOIUrl":"10.1364/BOE.545296","url":null,"abstract":"<p><p>Optical diffraction tomography enables label-free, 3D refractive index (RI) imaging of biological samples. We present a novel, cost-effective approach to ODT that employs a modular design incorporating a self-reference holographic capture module. This two-part system consists of an illumination module and a capture module that can be seamlessly integrated with any life-science microscope using an automated alignment protocol. The illumination module employs a galvo-scanner system, providing precise control over the angular illumination, while the capture module utilises the principle of self-reference off-axis holography. The design has a compact form factor, simple alignment, and reduced cost. Furthermore, our system offers the capability to switch between two imaging modalities, ODT and real-time synthetic aperture digital holographic microscopy (SA-DHM), a unique feature not found in other setups. Experimental results are provided using a kidney cancer cell line. Experimental results are provided using a kidney cancer cell line.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 1","pages":"57-67"},"PeriodicalIF":2.9,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-contact confocal calcium imaging of <i>in vivo</i> murine corneal nerves.","authors":"Matthew T McPheeters, Brecken J Blackburn, Eric Y Lu, Made Airanthi K Widjaja-Adhi, Andrew M Rollins, Marcin Golczak, William J Dupps, Michael W Jenkins","doi":"10.1364/BOE.543333","DOIUrl":"10.1364/BOE.543333","url":null,"abstract":"<p><p>Abnormal corneal nerve function and associated disease is a significant public health concern. It is associated with prevalent ocular surface diseases, including dry eye disease. Corneal nerve dysfunction is also a common side effect of refractive surgeries, as well as a symptom of diseases that cause peripheral neuropathies. Here, we demonstrate <i>in vivo</i> calcium imaging of mouse corneal nerves expressing GCaMP6f, a genetically encoded calcium indicator. A custom fluorescence imaging and stereotactic system was designed, allowing for non-contact imaging of the mouse cornea with an air objective. Dynamic imaging of neuronal activity is demonstrated in the various layers of the cornea and in response to local anesthetic administration. This approach demonstrates a less invasive means of assessing corneal nerve function than has been previously used, and has significant potential for studying the effects of ocular diseases, refractive surgeries, and peripheral neuropathies on corneal nerve function, as well as the effectiveness of various therapies to treat corneal nerve dysfunction.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 1","pages":"1-11"},"PeriodicalIF":2.9,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical components of corneal stroma: a study on myopia classification based on Raman spectroscopy and deep learning methods.","authors":"Zhe Yu, Yong Li, Tingyan Xing, Ming Han, Yaohua Zhang, Jinrong Gao, Jing Du, Jing Li, Qi Zeng, Xueli Chen","doi":"10.1364/BOE.539721","DOIUrl":"10.1364/BOE.539721","url":null,"abstract":"<p><p>The study aimed to identify differences in the biochemical composition of corneal stroma lenses across varying degrees of myopia using Raman spectrum characteristics. Corneal stroma lens samples from 38 patients who underwent small incision lens extraction (SMILE) surgery, were categorized into low (n = 9, spherical power <math><mo>≧</mo></math> -3.00D), moderate (n = 23, spherical power < -3.00D and > -6.00D), and high myopia (n = 6, spherical power ≦-6.00D) groups. A custom-built microscopic confocal Raman system (MCRS) was used to collect Raman spectra, which were processed by smoothing, denoising, and baseline calibrating to refine raw data. Independent sample t-tests were used to analyze spectral feature peaks among sample types. Significant differences (<i>P</i> < 0.001) were found in multiple Raman spectral characteristic peaks (854 cm^-1, 937 cm^-1, 1002 cm^-1, 1243 cm^-1, 1448 cm^-1, and 2940 cm^-1) between low and high myopia samples, particularly at 2940 cm^-1. Differences were also found between low and moderate, and moderate and high myopia samples, although fewer than between low and high myopia samples. The three-classification model, particularly with PLS-KNN training, exhibited superior discriminative performance with accuracy rates of 95%. Similarly, the two-classification model for low and high myopia achieved high accuracy with PLS-KNN (94.4%) compared to PCA-KNN (93.3%). PLS dimensionality reduction slightly outperformed PCA, enhancing classification accuracy. In addition, in both reduction methods, the KNN algorithm demonstrated the highest accuracy and performance. The optimal PLS-KNN classification model showed AUC values of 0.99, 0.98, and 1.00 for ROC curves corresponding to low, moderate, and high myopia, respectively. Classification accuracy rates were 89.7% and 96.9%, and 100% for low and high myopia, respectively. For the two-classification model, accuracy reached 94.4% with an AUC of 0.98, indicating strong performance in distinguishing between high and low myopic corneal stroma. We found significant biochemical differences such as collagen, lipids, and nucleic acids in corneal stroma lenses across varying degrees of myopia, suggesting that Raman spectroscopy holds substantial potential in elucidating the pathogenesis of myopia.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 1","pages":"28-41"},"PeriodicalIF":2.9,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Camera-on-tip endoscope for <i>in vivo</i> cardiovascular diagnostics and surgical guidance.","authors":"Simon T Sørensen, Walter Messina, Lorenzo Niemitz, Claire O'Dowling, Piotr Buszman, Stefan Andersson-Engels, Ray Burke","doi":"10.1364/BOE.543373","DOIUrl":"10.1364/BOE.543373","url":null,"abstract":"<p><p>Cardiovascular imaging with camera-on-tip endoscopes has the potential to provide physiologically relevant data on the tissue state and device placement that can improve clinical outcomes. In this work, we review the unmet clinical need for image-based <i>in vivo</i> cardiovascular diagnostics and guidance for minimally invasive procedures. We present a 7 Fr camera-on-tip endoscope with fibre-coupled multispectral illumination that includes methods for imaging in a blood-filled field of view (FOV). We demonstrate that the endoscope can be navigated from the femoral artery to cardiac regions such as the left atrium and left ventricle in a porcine model, where <i>in vivo</i> images of the cardiac walls are recorded. We further show that physiologically relevant parameters such as heart rate and respiration can be extracted from the images and that changes to tissue state can be inferred from the imaging data. Finally, a methodology for merging the imaging data with diffuse reflection spectroscopy (DRS) recorded through the optical fibre is outlined.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 1","pages":"12-27"},"PeriodicalIF":2.9,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of capillaries and small arterioles to full-field flicker is not dependent on local ganglion cell thickness.","authors":"Marcelina Sobczak, Brittany R Walker, Thomas Gast, Nicholas Cassavaugh, Alessandra Carmichael-Martins, Stephen A Burns","doi":"10.1364/BOE.544772","DOIUrl":"10.1364/BOE.544772","url":null,"abstract":"<p><p>To measure the influence of ganglion cell layer (GCL) thickness on the changes in size and red blood cell (RBC) flow in small retinal vessels evoked by full-field flicker. We used a dual-beam adaptive optics scanning laser ophthalmoscope to image 11 healthy young controls in two retinal areas with significantly different GCL thicknesses. All capillaries and arterioles of the superficial vascular plexus were responsive to the flicker stimulation. Average lumen dilation and RBC flow changes were greater in capillaries than in arterioles (vasodilation: 10.9%, 6.7%; RBC flow: 51%, 38%, respectively). No statistically significant differences regarding relative lumen diameter, RBC velocity, or RBC flow were found with respect to GCL thickness, or vessel size.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 1","pages":"42-56"},"PeriodicalIF":2.9,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}