Biomedical optics express最新文献

{"title":"AI-based 3D analysis of retinal vasculature associated with retinal diseases using OCT angiography.","authors":"Yu Liu, Zhenfei Tang, Chao Li, Zhengwei Zhang, Yaqin Zhang, Xiaogang Wang, Zhao Wang","doi":"10.1364/BOE.534703","DOIUrl":"10.1364/BOE.534703","url":null,"abstract":"<p><p>Retinal vasculature is the only vascular system in the human body that can be observed in a non-invasive manner, with a phenotype associated with a wide range of ocular, cerebral, and cardiovascular diseases. OCT and OCT angiography (OCTA) provide powerful imaging methods to visualize the three-dimensional morphological and functional information of the retina. In this study, based on OCT and OCTA multimodal inputs, a multitask convolutional neural network model was built to realize 3D segmentation of retinal blood vessels and disease classification for different retinal diseases, overcoming the limitations of existing methods that can only perform 2D analysis of OCTA. Two hundred thirty sets of OCT and OCTA data from 109 patients, including 138,000 cross-sectional images in normal and diseased eyes (age-related macular degeneration, retinal vein occlusion, and central serous chorioretinopathy), were collected from four commercial OCT systems for model training, validation, and testing. Experimental results verified that the proposed method was able to achieve a DICE coefficient of 0.956 for 3D segmentation of blood vessels and an accuracy of 91.49% for disease classification, and further enabled us to evaluate the 3D reconstruction of retinal vessels, explore the interlayer connections of superficial and deep vasculatures, and reveal the 3D quantitative vessel characteristics in different retinal diseases.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"15 11","pages":"6416-6432"},"PeriodicalIF":2.9,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proof of concept validation of bioresorbable optical fibers for diffuse correlation spectroscopy.","authors":"Jawad T Pandayil, Nadia G Boetti, Davide Janner, Turgut Durduran, Lorenzo Cortese","doi":"10.1364/BOE.540137","DOIUrl":"10.1364/BOE.540137","url":null,"abstract":"<p><p>Optical quality bioresorbable materials have been gaining interest in recent years for various interstitial biomedical/medical application. An example of this is when the implant gradually dissolves in the body, providing physiological information over extended periods of time, hence reducing the need for revision surgeries. This study reports for the first time the in-house fabrication of single mode (at 785 nm) calcium phosphate glass (CPG) based bioresorbable optical fibers and investigates their suitability for microvascular blood flow monitoring using diffuse correlation spectroscopy (DCS). <i>Ex vivo</i> experiments in liquid phantom and non-invasive <i>in vivo</i> experiments on the human forearm muscle were conducted using multimode and single mode CPG bioresorbable optical fibers. The retrieved flow index from the correlation curves acquired using CPG fibers was in good agreement with that obtained using standard silica (Si) fibers, both <i>ex vivo</i> and <i>in vivo.</i> The results demonstrate the potential of CPG optical fibers for further exploration.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"15 11","pages":"6384-6398"},"PeriodicalIF":2.9,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-destructive viability assessment of cancer cell spheroids using dynamic optical coherence tomography with trypan blue validation.","authors":"Ko Hui Tan, Joel Lang Yi Ang, Alexander Si Kai Yong, Stefanie Zi En Lim, Jessica Sze Jia Kng, Kaicheng Liang","doi":"10.1364/BOE.533339","DOIUrl":"10.1364/BOE.533339","url":null,"abstract":"<p><p>3D cell cultures are widely used in biomedical research for the recapitulation of <i>in vivo</i> microenvironments. Viability assessment and monitoring of these intricate conformations remain an open problem as standard cell viability protocols based on colorimetry or microscopy are not directly applicable to intact 3D samples. Optical coherence tomography (OCT) has been explored extensively for subsurface structural and quasi-functional analysis of 3D cell cultures and tissue. Recent studies of dynamic OCT as a source of cellular contrast have found qualitative associations with necrosis in cell spheroids, suggesting potential as a viability marker. We present empirical and validated evidence for dynamic OCT as a quantitative indicator of cell viability in 3D cultures. We analysed over 240 MCF-7 cancer cell spheroids with dynamic OCT and corresponding viability measurements using the trypan blue exclusion assay. Significant effects of common reagents dimethyl sulfoxide (DMSO) and phosphate-buffered saline (PBS) on OCT readouts were noted. We proposed a regression-based OCT brightness normalisation technique that removed reagent-induced OCT intensity biases and helped improve correspondence to the viability assay. These results offer a quantitative biological foundation for further advances of dynamic OCT as a novel non-invasive modality for 3D culture monitoring.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"15 11","pages":"6370-6383"},"PeriodicalIF":2.9,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Realtime and noninvasive assessment of endotracheal tube displacement using near-infrared and visible cameras.","authors":"Tongtong Lu, Pawjai Khampang, Ahmed Beydoun, Anna Berezovsky, Rebecca Rohde, Wenzhou Hong, Joseph E Kerschner, Bing Yu","doi":"10.1364/BOE.531815","DOIUrl":"10.1364/BOE.531815","url":null,"abstract":"<p><p>Endotracheal tube (ETT) intubation is a medical procedure routinely used for achieving mechanical ventilation in critically ill patients. Appropriate ETT placement is crucial as undetected tube migration may cause multiple complications or even fatalities. Therefore, prompt detection of unplanned movement of the ETT and immediate action to restore proper placement are essential to ensure patient safety. Despite this necessity, there is not a widely adopted tool for real-time assessment of ETT displacement. We have developed a device, a dual-camera endotracheal tube or DC-ETT, to address this unmet clinical need. This device uses a near-infrared (NIR) LED and a side-firing optical fiber embedded in the side of an ETT to light up the tracheal tissue and a visible and NIR camera module for the displacement detection. The NIR camera tracks the movement of the NIR pattern on the skin, while the visible camera is used to correct the body movements. The efficacy of the DC-ETT was assessed in two piglets with a linear displacement sensor as reference. A mean discrepancy of less than 0.5 mm between the DC-ETT and reference sensor was observed within a displacement range of ±15 mm. The results suggest that the DC-ETT can potentially provide a simple and cost-effective solution for real-time monitoring of ETT displacements in operating rooms, intensive care units, and emergency departments.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"15 11","pages":"6355-6369"},"PeriodicalIF":2.9,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimodal photoacoustic microscopy and optical coherence tomography ocular biomarker imaging in Alzheimer's disease in mice.","authors":"Tianqu Zhai, Wei Qian, Yannis M Paulus, Xueding Wang, Wei Zhang","doi":"10.1364/BOE.532042","DOIUrl":"10.1364/BOE.532042","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is a neurodegenerative disease characterized by amyloid beta (Aβ)-containing extracellular plaques and tau-containing intracellular neurofibrillary tangles. Reliable and more accessible biomarkers along with associated imaging methods are essential for early diagnosis and to develop effective therapeutic interventions. Described here is an integrated photoacoustic microscopy (PAM) and optical coherence tomography (OCT) dual-modality imaging system for multiple ocular biomarker imaging in an AD mouse model. Anti-Aβ-conjugated Au nanochains (AuNCs) were engineered and administered to the mice to provide molecular contrast of Aβ. The retinal vasculature structure and Aβ deposition in AD mice and wild-type (WT) mice were imaged simultaneously by dual-wavelength PAM. OCT distinguished significant differences in retinal layer thickness between AD and WT animals. With the unique ability of imaging the multiple ocular biomarkers via a coaxial multimodality imaging system, the proposed system provides a new tool for investigating the progression of AD in animal models, which could contribute to preclinical studies of AD.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"15 11","pages":"6340-6354"},"PeriodicalIF":2.9,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Video-rate two-photon microendoscopy using second harmonic resonance fiber scanning.","authors":"Kai Zhang, Guillaume Ducourthial, Taresh Sharan, Arvind Mohan, Jiasen Hou, Sudip Timilsina, Rongguang Liang, Frederic Louradour, Bryan Q Spring","doi":"10.1364/BOE.534399","DOIUrl":"10.1364/BOE.534399","url":null,"abstract":"<p><p>We present a 2.5-mm-diameter resonant fiber scanning two-photon microendoscope with a 30-mm long forward-viewing rigid probe tip that enables video-rate imaging (20 Hz frame rate) suitable for hand-held imaging of tissues without motion artifacts. Higher-order harmonic oscillation scanning techniques are developed to significantly increase the frame rate compared to prior published fiber scanning microendoscopy designs while maintaining the field-of-view (∼125 µm), the optical resolution (1.2 µm lateral and 10.9 µm axial resolution, full width at half maximum), and the spatial sampling (1250 circumferential pixels per spiral × 20 radial pixels over the diameter; 210 spirals per frame, ∼4 spiral samples per resolvable pixel) compared to a traditional scan using the fundamental resonance. 3D printed mounts were created to reduce the cost and simplify the fabrication for the fiber scanner without compromising performance or stability (<0.3 µm drift over 84 hours). A custom long-wavelength (∼1.08 µm) femtosecond fiber laser is coupled into several meters of fiber to realize a flexible, hand-held device for long-wavelength multiphoton microendoscopy.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"15 11","pages":"6324-6339"},"PeriodicalIF":2.9,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-mode OCT/fluorescence system for monitoring the morphology and metabolism of laser-printed 3D full-thickness skin equivalents.","authors":"Arooj Khalid, Viktor Dremin, Ayman El-Tamer, Maria Surnina, Celine Lancelot, Edik Rafailov, Sergei Sokolovski","doi":"10.1364/BOE.510610","DOIUrl":"10.1364/BOE.510610","url":null,"abstract":"<p><p>The 3D structure of native human skin is fundamental for studying skin health, diseases, wound healing, and for testing the safety of skin care products, as well as personalized treatments for skin conditions. Tissue regeneration, driven by tissue engineering, often involves creating full-thickness skin equivalents (FSE), which are widely used for developing both healthy and diseased skin models. In this study, we utilized human skin cell lines to create FSE. We designed high-resolution 3D scaffolds to support the growth and maturation of these skin models. Additionally, we developed and validated a cost-effective, custom-built system combining fluorescence spectroscopy (FS) and optical coherence tomography (OCT) for non-destructive analysis of the metabolism and morphology of 3D FSEs. This system proved highly sensitive in detecting fluorescence from key metabolic co-enzymes (NADH and FAD) in solutions and cell suspensions, while OCT provided adequate resolution to observe the morphology of FSEs. As a result, both the 3D FSE model and the dual-mode optical system hold significant potential for use in 3D bioprinting of biological tissues, as well as in the development of cosmetics, drugs, and in monitoring their maturation over time.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"15 11","pages":"6299-6312"},"PeriodicalIF":2.9,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved tracking of corneal immune cell dynamics using <i>in vivo</i> confocal microscopy.","authors":"Phillip Bedggood, Mengliang Wu, Xinyuan Zhang, Rajni Rajan, Ching Yi Wu, Senuri Karunaratne, Andrew B Metha, Scott N Mueller, Holly R Chinnery, Laura E Downie","doi":"10.1364/BOE.536553","DOIUrl":"10.1364/BOE.536553","url":null,"abstract":"<p><p><i>In vivo</i> confocal microscopy (IVCM) is a widely used technique for imaging the cornea of the eye with a confocal scanning light ophthalmoscope. Cellular resolution and high contrast are achieved without invasive procedures, suiting the study of living humans. However, acquiring useful image data can be challenging due to the incessant motion of the eye, such that images are typically limited by noise and a restricted field of view. These factors affect the degree to which the same cells can be identified and tracked over time. To redress these shortcomings, here we present a data acquisition protocol together with the details of a free, open-source software package written in Matlab. The software package automatically registers and processes IVCM videos to significantly improve contrast, resolution, and field of view. The software also registers scans acquired at progressive time intervals from the same tissue region, producing a time-lapsed video to facilitate visualization and quantification of individual cell dynamics (e.g., motility and dendrite probing). With minimal user intervention, to date, this protocol has been employed to both cross-sectionally and longitudinally assess the dynamics of immune cells in the human corneal epithelium and stroma, using a technique termed functional in vivo confocal microscopy (Fun-IVCM) in 68 eyes from 68 participants. Using the custom software, registration of 'sequence scan' data was successful in 97% of videos acquired from the corneal epithelium and 93% for the corneal stroma. Creation of time-lapsed videos, in which the averages from single videos were registered across time points, was successful in 93% of image series for the epithelium and 75% of image series for the stroma. The reduced success rate for the stroma occurred due to practical difficulties in finding the same tissue between time points, rather than due to errors in image registration. We also present preliminary results showing that the protocol is well suited to <i>in vivo</i> cellular imaging in the retina with adaptive optics scanning laser ophthalmoscopy (AOSLO). Overall, the approach described here substantially improves the efficiency and consistency of time-lapsed video creation to enable non-invasive study of cell dynamics across diverse tissues in the living eye.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"15 11","pages":"6277-6298"},"PeriodicalIF":2.9,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an ultrafast laser system based on a Yb-doped fiber and a Yb:YAG thin-rod for the preclinical study of pigmented lesions treatment using a Hartley guinea pig.","authors":"Jun Wan Kim, Guang-Hoon Kim, Seolwon Park, Byung Cheol Park, Soon Re Kim, Juhee Kim, Juhee Yang","doi":"10.1364/BOE.540536","DOIUrl":"10.1364/BOE.540536","url":null,"abstract":"<p><p>In this research, we developed an ultrafast laser system based on a Yb-doped fiber oscillator and Yb:YAG thin-rod amplifier to investigate the efficacy of the laser for the treatment of pigmented lesions. The developed laser exhibited an output power of 22.7 W, center wavelength of 1030 nm, repetition rate of 495 kHz, pulse energy of 45.9 µJ, and pulse duration of 1.56 ps, respectively. For a compact and stable chirped pulse amplification system, a chirped fiber Bragg grating (CFBG) stretcher and a chirped volume Bragg grating (CVBG) compressor, both with fixed dispersion, were used. The dispersion of the total laser systems was precisely compensated by adjusting the length of the passive fiber and utilizing the self-phase modulation effect of the fiber amplifier. The developed ultrafast laser system was then applied in preclinical studies for the treatment of pigmented lesions in a guinea pig model. Three colored squares, each measuring approximately 15 × 15 mm, were treated by scanning a focused beam with varying laser fluences ranging from 0.5 to 2 J/cm², using wavelengths of 515 nm and 1030 nm. The colorimeter measurements, which were performed 1-5 weeks after laser treatment, indicated that the laser was effective in reducing pigment, particularly black and blue pigments at higher fluences. This research represents the first trial of a preclinical study on pigmented lesions using an ultrafast laser system with a pulse duration below 10 ps, shorter than the stress relaxation time of 10 nm melanin granules. The results are meaningful as they offer valuable insights into the effectiveness of ultrafast laser therapy.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"15 11","pages":"6313-6323"},"PeriodicalIF":2.9,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diffuse correlation tomography: a technique to characterize tissue blood flow abnormalities in benign and malignant breast lesions.","authors":"Ruizhi Zhang, Jianju Lu, Wenqi Di, Zhiguo Gui, Shun Wan Chan, Fengbao Yang, Yu Shang","doi":"10.1364/BOE.535330","DOIUrl":"10.1364/BOE.535330","url":null,"abstract":"<p><p>Accurate assessment and quantification of neoangiogenesis associated with breast cancer could be potentially used to improve the sensitivity and specificity of non-invasive diagnosis, as well as predict outcomes and monitor treatment effects. In this study, we adapted an emerging technology, namely diffuse correlation tomography (DCT), to image microvascular blood flow in breast tissues and evaluate the potential for discriminating between benign and malignant lesions. A custom-made DCT system was designed for breast blood flow imaging, with both the source-detector array and reconstruction algorithm optimized to ensure precise imaging of breast blood flow. The global features and local features of three-dimensional blood flow images were extracted from the relative blood flow index (rBFI), which was obtained from most of the breasts targeted to the lesion. A total of 37 women with 19 benign and 18 malignant lesions were included in the study. Significant differences between malignant and benign groups were found in 12 image features. Moreover, when selecting the lesion mean relative blood flow index (MrBFI) as a single indicator, the malignant and benign tumors were discriminated with an accuracy of 89.2%. The blood flow features were found to successfully identify malignant and benign tumors, suggesting that DCT, as an alternate functional imaging modality, has the potential to be translated into clinical practice for diagnosis and assessment of breast cancers. There is potential to reduce the need for biopsy of benign lesions by improving the specificity of diagnostic imaging, as well as monitoring response to breast cancer treatment.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"15 11","pages":"6259-6276"},"PeriodicalIF":2.9,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142650569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}