Biomedical optics express最新文献_第8页

Biomedical optics: illuminating paths to enhance health equity in the Philippines. 生物医学光学：照亮提高菲律宾卫生公平的道路。

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-26 DOI: 10.1364/boe.531499

Phoebe Nicole G Perez,Karell Jo Angelique C Calpito,Sarah Jane A Jimenez

引用次数: 0

Ex vivo human teeth imaging with various photoacoustic imaging systems. 利用各种光声成像系统对人体牙齿进行活体成像。

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-23 DOI: 10.1364/boe.531436

Vijitha Periyasamy,Katherine Gisi,Manojit Pramanik

{"title":"Ex vivo human teeth imaging with various photoacoustic imaging systems.","authors":"Vijitha Periyasamy,Katherine Gisi,Manojit Pramanik","doi":"10.1364/boe.531436","DOIUrl":"https://doi.org/10.1364/boe.531436","url":null,"abstract":"Dental caries cause pain and if not diagnosed, it may lead to the loss of teeth in extreme cases. Dental X-ray imaging is the gold standard for caries detection; however, it cannot detect hidden caries. In addition, the ionizing nature of X-ray radiation is another concern. Hence, other alternate imaging modalities like photoacoustic (PA) imaging are being explored for dental imaging. Here, we demonstrate the feasibility of acoustic resolution photoacoustic microscopy (ARPAM) to image a tooth with metal filling, circular photoacoustic computed tomography (cPACT) to acquire images of teeth with caries and pigmentation, and linear array-based photoacoustic imaging (lPACT) of teeth with caries and pigmentation. The cavity measured with lPACT imaging is compared with the X-ray computed tomography image. The metal filling and its boundaries are clearly seen in the ARPAM image. cPACT images at 1064 nm were a better representative of the tooth surface compared to the images acquired at 532 nm. It was possible to detect the cavities present in the dentine when lPACT imaging was used. The PA signal from the pigmented caries on the lateral surface (occlusion view) of the tooth was high when imaged using the lPACT system.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"115 1","pages":"5479-5490"},"PeriodicalIF":3.4,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational approach for correcting defocus and suppressing speckle noise in line-field optical coherence tomography images. 校正线场光学相干断层扫描图像中的散焦和抑制斑点噪声的计算方法。

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-23 DOI: 10.1364/boe.530569

Nima Abbasi,Keyu Chen,Alexander Wong,Kostadinka Bizheva

{"title":"Computational approach for correcting defocus and suppressing speckle noise in line-field optical coherence tomography images.","authors":"Nima Abbasi,Keyu Chen,Alexander Wong,Kostadinka Bizheva","doi":"10.1364/boe.530569","DOIUrl":"https://doi.org/10.1364/boe.530569","url":null,"abstract":"The trade-off between transverse resolution and depth-of-focus (DOF) typical for optical coherence tomography (OCT) systems based on conventional optics, prevents \"single-shot\" acquisition of volumetric OCT images with sustained high transverse resolution over the entire imaging depth. Computational approaches for correcting defocus and higher order aberrations in OCT images developed in the past require highly stable phase data, which poses a significant technological challenge. Here, we present an alternative computational approach to sharpening OCT images and reducing speckle noise, based on intensity OCT data. The novel algorithm uses non-local priors to model correlated speckle noise within a maximum a posteriori framework to generate sharp and noise-free images. The performance of the algorithm was tested on images of plant tissue (cucumber) and in-vivo healthy human cornea, acquired with line-field spectral domain OCT (LF-SD-OCT) systems. The novel algorithm effectively suppressed speckle noise and sharpened or recovered morphological features in the OCT images for depths up to 13×DOF (depth-of-focus) relative to the focal plane.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"23 1","pages":"5491-5504"},"PeriodicalIF":3.4,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probing diffusive media through speckle differencing. 通过斑点差分探测扩散介质

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-22 DOI: 10.1364/boe.531797

Muralidhar Madabhushi Balaji,Danyal Ahsanullah,Prasanna Rangarajan

引用次数: 0

CRISPR/Cas12a cleavage triggered nanoflower for fluorescence-free and target amplification-free biosensing of ctDNA in the terahertz frequencies. CRISPR/Cas12a 裂解触发纳米花，用于太赫兹频率下无荧光和无靶标放大的 ctDNA 生物传感。

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-21 DOI: 10.1364/boe.534511

Jingjing Zhao,Jianfang Zhu,Weiqiang Wang,Zhengfang Qian,Shuting Fan

{"title":"CRISPR/Cas12a cleavage triggered nanoflower for fluorescence-free and target amplification-free biosensing of ctDNA in the terahertz frequencies.","authors":"Jingjing Zhao,Jianfang Zhu,Weiqiang Wang,Zhengfang Qian,Shuting Fan","doi":"10.1364/boe.534511","DOIUrl":"https://doi.org/10.1364/boe.534511","url":null,"abstract":"The detection of tumor biomarkers in liquid biopsies requires high sensitivity and low-cost biosensing strategies. However, few traditional techniques can satisfy the requirements of target amplification-free and fluorescence-free at the same time. In this study, we have proposed a novel strategy for ctDNA detection with the combination of terahertz spectroscopy and the CRISPR/Cas12 system. The CRISPR/Cas12a system is activated by the target ctDNA, resulting in a series of reactions leading to the formation of an Au-Fe complex. This complex is easily extracted with magnets and when dropped onto the terahertz metamaterial sensor, it can enhance the frequency shift, providing sensitive and selective sensing of the target ctDNA. Results show that the proposed terahertz biosensor exhibits a relatively low detection limit of 0.8 fM and a good selectivity over interference species. This detection limit is improved by three orders of magnitude compared with traditional biosensing methods using terahertz waves. Furthermore, a ctDNA concentration of 100 fM has been successfully detected in bovine serum (corresponding to 50 fM in the final reaction system) without amplification.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"10 1","pages":"5400-5410"},"PeriodicalIF":3.4,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Image stitching algorithm for super-resolution localization microscopy combined with fluorescence noise prior. 结合荧光噪声先验的超分辨率定位显微镜图像拼接算法。

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-21 DOI: 10.1364/boe.534658

Yanzhu Chen,Zhiwang Xu,Shijie Ren,Zhen-Li Huang,Zhengxia Wang

{"title":"Image stitching algorithm for super-resolution localization microscopy combined with fluorescence noise prior.","authors":"Yanzhu Chen,Zhiwang Xu,Shijie Ren,Zhen-Li Huang,Zhengxia Wang","doi":"10.1364/boe.534658","DOIUrl":"https://doi.org/10.1364/boe.534658","url":null,"abstract":"Super-resolution panoramic pathological imaging provides a powerful tool for biologists to observe the ultrastructure of samples. Localization data can maintain the essential ultrastructural information of biological samples with a small storage space, and also provides a new opportunity for stitching super-resolution images. However, the existing image stitching methods based on localization data cannot accurately calculate the registration offset of sample regions with no or few structural points and thus lead to registration errors. Here, we proposed a stitching framework called PNanoStitcher. The framework fully utilizes the distribution characteristics of the background fluorescence noise in the stitching region and solves the stitching failure in sample regions with no or few structural points. We verified our method using both simulated and experimental datasets, and compared it with existing stitching methods. PNanoStitcher achieved superior stitching results on biological samples with no structural and few structural regions. The study provides an important driving force for the development of super-resolution digital pathology.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"8 1","pages":"5411-5428"},"PeriodicalIF":3.4,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bidirectional in-silico clearing approach for deep refractive-index tomography using a sparsely sampled transmission matrix. 利用稀疏采样透射矩阵进行深层折射率断层成像的双向硅内清除方法。

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-19 DOI: 10.1364/boe.524859

Osamu Yasuhiko,Kozo Takeuchi

{"title":"Bidirectional in-silico clearing approach for deep refractive-index tomography using a sparsely sampled transmission matrix.","authors":"Osamu Yasuhiko,Kozo Takeuchi","doi":"10.1364/boe.524859","DOIUrl":"https://doi.org/10.1364/boe.524859","url":null,"abstract":"Optical diffraction tomography (ODT) enables the label-free volumetric imaging of biological specimens by mapping their three-dimensional refractive index (RI) distribution. However, the depth of imaging achievable is restricted due to spatially inhomogeneous RI distributions that induce multiple scattering. In this study, we introduce a novel ODT technique named bidirectional in-silico clearing RI tomography. This method incorporates both forward and reversed in-silico clearing. For the reversed in-silico clearing, we have integrated an ODT reconstruction framework with a transmission matrix approach, which enables RI reconstruction and wave backpropagation from the illumination side without necessitating modifications to the conventional ODT setup. Furthermore, the framework employs a sparsely sampled transmission matrix, significantly reducing the requisite number of measurements and computational expenses. Employing this proposed technique, we successfully imaged a spheroid with a thickness of 263 µm, corresponding to 11.4 scattering mean free paths. This method was successfully applied to various biological specimens, including liver and colon spheroids, demonstrating consistent imaging performance across samples with varied morphologies.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"5 1","pages":"5296-5313"},"PeriodicalIF":3.4,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanically sheared axially swept light-sheet microscopy. 机械剪切轴向扫描光片显微镜。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2024-08-19 eCollection Date: 2024-09-01 DOI: 10.1364/BOE.526145

Jinlong Lin, Dushyant Mehra, Zach Marin, Xiaoding Wang, Hazel M Borges, Qionghua Shen, Seweryn Gałecki, John Haug, Derek H Abbott, Kevin M Dean

引用次数: 0

Sensitivity analysis of transabdominal fetal pulse oximetry using MRI-based simulations. 基于磁共振成像模拟的经腹胎儿脉搏血氧仪敏感性分析。

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-19 DOI: 10.1364/boe.531149

Jingyi Wu,Gopika Satish,Alexander Ruesch,Baptiste Jayet,Katarzyna Komolibus,Stefan Andersson-Engels,Martin P Debreczeny,Jana M Kainerstorfer

引用次数: 0

Acoustomotive diffuse correlation spectroscopy for sensing mechanical stiffness in tissue-mimicking phantoms. 声学扩散相关光谱法用于感知组织模拟模型中的机械刚度。

IF 3.4 2区医学

Biomedical optics express Pub Date : 2024-08-19 DOI: 10.1364/boe.531963

Wenqi Di,Ruizhi Zhang,Zhiguo Gui,Yu Shang

{"title":"Acoustomotive diffuse correlation spectroscopy for sensing mechanical stiffness in tissue-mimicking phantoms.","authors":"Wenqi Di,Ruizhi Zhang,Zhiguo Gui,Yu Shang","doi":"10.1364/boe.531963","DOIUrl":"https://doi.org/10.1364/boe.531963","url":null,"abstract":"Many diseases, such as inflammation, dropsy, or tumors, often cause alterations in the mechanical stiffness of human tissues. Ultrasound-based techniques are commonly adopted in clinics for stiffness assessment, whereas optical methodologies hold promise for sensing strain changes and providing optical information pertaining to the microcirculatory network, thereby facilitating comprehensive measurements of tissue physiopathology. Diffuse correlation spectroscopy (DCS), an emerging dynamic light scattering technique, has been used to capture the enhanced motion of light scatterers induced by acoustic radiation force (ARF). Theoretically, the amplitude of this enhanced scatterers motion is related to the medium stiffness. Based on this relationship, we report a light coherent technique that combines ARF and DCS to qualitatively evaluate changes in the stiffness of medium. We experimentally demonstrate the accuracy and feasibility of this technique for probing stiffness in homogeneous phantom by comparing it with independent ultrasound methods. Additionally, we explore a potential application of this technique in distinguishing between fluid filled lesion and homogeneous tissue through heterogeneous phantom experiments. This unique combination of ARF and DCS, namely, acoustomotive DCS (AM-DCS), would provide an alternative way to measure particle-motion related stiffness, thereby assisting in the diagnosis and treatment of diseases.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"1 1","pages":"5328-5348"},"PeriodicalIF":3.4,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0