Biomedical optics express最新文献

{"title":"Classifying retinal diseases via pyramid vision graph convolutional network for optical coherence tomography images.","authors":"Jin Qian, Lei Tao, Changhao Gong, Jun Xu, Yuemei Luo","doi":"10.1364/BOE.558731","DOIUrl":"10.1364/BOE.558731","url":null,"abstract":"<p><p>Recent advancements have seen a significant focus on using deep neural networks for classifying retinal diseases in optical coherence tomography (OCT) images. However, traditional deep neural networks treat images as grid or sequential structures, limiting their flexibility in capturing irregular and complex objects, resulting in suboptimal performance in practical applications. To address this issue, we propose a novel visual neural network model with a pyramid structure, called pyramid vision graph convolutional networks (PVGCN). This model enhances the correlations between structures by segmenting images into multiple nodes and connecting the nearest nodes. Specifically, it consists of two core components: 1) vision graph block and 2) pyramid structure. The vision graph block, composed of a grapher block and a feed-forward network (FFN), uses graph convolution methods to divide the image into multiple regions, treating them as nodes and representing the image as graph data. The graph constructed based on nodes can capture relationships between nodes without positional restrictions, better representing the irregular structure of retinal tissue. The FFN module improves the over-smoothing phenomenon in the grapher stage, enabling more accurate classification. The pyramid structure decomposes OCT images into a series of sub-images at different scales, integrating features at different scales to obtain a comprehensive feature representation of retinal hierarchical structure information. This structure can replace the extraction of higher-dimensional features in a large model by integrating features at different scales, significantly reducing the number of parameters. We conducted extensive experiments on two different datasets. The experimental results show that the proposed PVGCN achieved accuracies of 0.9954 and 0.9787 on the two datasets, respectively, surpassing existing methods. Additionally, the model demonstrated recognition capabilities comparable to those of human experts in the experiments, effectively identifying retinal diseases in OCT images.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 6","pages":"2312-2326"},"PeriodicalIF":2.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-resolution quantitative phase imaging via vortex beam speckle illumination.","authors":"Shengqiang Zhong, Hongwei Zou, Chao Hou, Fan Yang, Kaibin Zeng, Yuhan Liu, Yongsheng Huang, Xiantao Jiang","doi":"10.1364/BOE.560024","DOIUrl":"10.1364/BOE.560024","url":null,"abstract":"<p><p>This study introduces a vortex beam speckle imaging system for quantitative phase imaging (QPI) with high lateral resolution. By introducing vortex beams for non-diffracting speckle field regulation, the speckle size can be significantly reduced from 116.32 μm to 11.07 μm. With these advantages, the proposed imaging system has shown 1.52 folds of lateral resolution improvement compared to a traditional coherent imaging system. Furthermore, the intensity signal-to-noise ratio of the imaging system has also been improved from 13.26 dB to 30.62 dB. Transport-of-intensity equation (TIE) phase retrieval algorithms were applied to standard quantitative phase targets, and red blood cell samples were used to demonstrate the system's precise phase retrieval capability, indicating its potential applications for label-free, non-invasive biomedical imaging.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 6","pages":"2275-2282"},"PeriodicalIF":2.9,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265489/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of material property modifications on optical performance: a multidisciplinary study in the human eye under different intraocular pressures.","authors":"Saeed Zahabi, Milad Salimibani, Agnieszka Jóźwik, Magdalena Asejczyk","doi":"10.1364/BOE.555315","DOIUrl":"10.1364/BOE.555315","url":null,"abstract":"<p><p>This study integrates mechanical and optical analyses to provide a robust framework for determining eye parameters and predicting visual changes under specific environmental or physical conditions. A 3D microstructural finite element model of the healthy human eye with elastic and viscoelastic properties was created and subjected to idealized and physiological intraocular pressure (IOP) load boundaries. It was our goal to investigate how the cornea, limbus, zonulas, and lens properties changed and how these changes affected key optical parameters such as focal length, Strehl ratio, and the RMS wave. The findings underscore the intricate role these factors play, highlight the significant role limbus play in preserving optimal visual function, and reveal biomechanical thresholds essential for maintaining ocular stability. A non-linear relationship was observed, in which moderate increases in stiffness of the limbus enhance stability while excessive stiffness compromises adaptability. This interdisciplinary approach advances our understanding of biomechanical and optical coupling, which is essential for vision optimization.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 6","pages":"2254-2274"},"PeriodicalIF":2.9,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265445/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-low limit of detection of a carcinoembryonic antigen fiber sensor based on microfiber.","authors":"Jinlong Wan, Senyu Wang, Sirong Zhou, Hongxiao Qiu, Rongpei Lu, Mingrui Jiang, Jianfeng Li, Yi Huang, Lulu Cai","doi":"10.1364/BOE.559885","DOIUrl":"10.1364/BOE.559885","url":null,"abstract":"<p><p>Fiber optic sensors have been widely applied to address issues such as cardiovascular diseases, pathogen detection, and cancer biomarker detection. In this work, an ultra-low limit of detection, high-specificity, and label-free optical fiber biosensor for the detection of carcinoembryonic antigen (CEA) was proposed and experimentally demonstrated. A microfiber interferometer with refractive index sensitivity of up to 1915.955 nm/RIU was employed as the sensor head. The sensitivity of the sensor was further enhanced by the incorporation of molybdenum disulfide (MoS₂), which facilitated the immobilization of CEA antibodies as specific detection markers on the microfiber surface. Experimental results showed that the proposed sensor achieved a detection limit of 15.86 fg/mL. The sensitivity of the biosensor was 4.727 nm/log(mg/mL). Notably, this biosensor demonstrated excellent specificity and label-free operation and enabled the detection of low-concentration CEA in approximately 15 minutes. The proposed micro fiber CEA sensor provided a highly sensitive and low-cost method for the early diagnosis of cancer.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 6","pages":"2232-2242"},"PeriodicalIF":2.9,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283100/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-free sensing technology of two Staphylococcus species based on a terahertz metasurface.","authors":"Hui Zhong, Jingle Li, Jinghao Jiang, Xunpei Xu, Dongdong Zhang, Liwei Song, Rui Gao, Ye Tian","doi":"10.1364/BOE.560412","DOIUrl":"10.1364/BOE.560412","url":null,"abstract":"<p><p>Staphylococcus epidermidis (SE) and Staphylococcus aureus (SAU), comprising 50% of pathogenic bacteria in clinical blood isolates, demand precise detection for infection control. We present a label-free terahertz (THz) metasurface biosensor enabling simultaneous quantification and speciation of these Gram-positive pathogens. A tailored metasurface enhances THz wave-bacteria interaction through sharp resonance, creating quantitative correlations between resonance shifts and bacterial fluid dosage. Distinct linear regression slopes (SAU: 105.90 GHz/μL, SE: 45.14 GHz/μL) permit species differentiation, achieving specificity without biochemical labeling. This platform eliminates complex surface functionalization, reducing preparation time compared to ELISA-based methods while maintaining high sensitivity (theoretically 135.1 GHz/RIU, at a thickness of 3 μm, and experimentally 556 GHz/cell μm^-2 (SAU) and 237 GHz/cell μm^-2 (SE)). The technology's dual detection-differentiation capability, combined with its operational simplicity and cost-effectiveness, demonstrates transformative potential for clinical diagnostics and food safety monitoring, particularly in resource-limited settings requiring rapid pathogen screening.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 6","pages":"2210-2216"},"PeriodicalIF":2.9,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing the acquisition speed in oblique plane microscopy via aliasing.","authors":"Conor McFadden, James D Manton, Holly Merta, Reto Fiolka","doi":"10.1364/BOE.555473","DOIUrl":"https://doi.org/10.1364/BOE.555473","url":null,"abstract":"<p><p>Oblique plane microscopy (OPM), a variant of light-sheet fluorescence microscopy (LSFM), enables rapid volumetric imaging without mechanically scanning the sample or an objective. In an OPM, the sample space is mapped to a distortion-free image space via remote focusing, and the oblique light-sheet plane is mapped onto a camera via a tilted tertiary imaging system. As a result, the 3D point-spread function and optical transfer function (OTF) are tilted to the optical axis of the tertiary imaging system. To satisfy Nyquist sampling, small scanning steps are required to encompass the tilted 3D OTF, slowing down acquisition and increasing sample exposure. Here, we show that a judicious amount of under-sampling can lead to a form of aliasing in OPM that can be recovered without a loss of spatial resolution while minimizing artifacts. The resulting speed gains depend on the optical parameters of the system and reach 2-4-fold in our demonstrations. We leverage this method for rapid subcellular 3D imaging of mitochondria and the endoplasmic reticulum.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 4","pages":"1742-1751"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12047713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential artery-vein analysis in OCTA for predicting the anti-VEGF treatment outcome of diabetic macular edema.","authors":"Mansour Abtahi, Albert K Dadzie, Behrouz Ebrahimi, Boda Huang, Yi-Ting Hsieh, Xincheng Yao","doi":"10.1364/BOE.557748","DOIUrl":"https://doi.org/10.1364/BOE.557748","url":null,"abstract":"<p><p>This study evaluates the role of differential artery-vein (AV) analysis in optical coherence tomography angiography (OCTA) for treatment outcome prediction of diabetic macular edema (DME). Deep learning AV segmentation in OCTA enabled the robust extraction of quantitative AV features, including perfusion intensity density (PID), blood vessel density (BVD), vessel skeleton density (VSD), vessel area flux (VAF), blood vessel caliber (BVC), blood vessel tortuosity (BVT), and vessel perimeter index (VPI). Support vector machine (SVM) classifiers were employed to predict changes in best-corrected visual acuity (BCVA) and central retinal thickness (CRT). Comparative analysis revealed that differential AV analysis significantly enhanced prediction performance, with BCVA accuracy improved from 70.45% to 86.36% and CRT accuracy enhanced from 68.18% to 79.55% compared to traditional OCTA analysis. These findings underscore the potential of AV analysis as a transformative tool for advancing personalized therapeutic strategies and improving clinical decision-making in managing DME.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 4","pages":"1732-1741"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12047724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FLIMB: fluorescence lifetime microendoscopy for metabolic and functional imaging of femoral marrow at subcellular resolution.","authors":"Alexander F Fiedler, Ruth Leben, Herbert Stürmer, Robert Günther, Romano Matthys, Reto Nützi, Anja E Hauser, Raluca A Niesner","doi":"10.1364/BOE.549311","DOIUrl":"https://doi.org/10.1364/BOE.549311","url":null,"abstract":"<p><p>Intravital imaging of bone marrow provides a unique opportunity to study cellular dynamics and their interaction with the tissue microenvironment, which governs cell functions and metabolic profiles. To optically access the deep marrow of long bones, we previously developed a microendoscopy system for longitudinal two-photon fluorescence imaging of the murine femur. However, this does not provide information on cell functions or metabolism, for which quantification fluorescence lifetime imaging (FLIM) has proven to be a versatile tool. We present and characterize FLIMB, an adapted GRIN-based microendoscopic system capable of performing reliable, co-registered TCSPC-based two-photon excited FLIM and fluorescence imaging in the femur of fluorescent reporter mice, at sub-cellular resolution. Using FLIMB, we demonstrate metabolic imaging via NAD(P)H-FLIM and intracellular Ca²⁺ signaling via FRET-FLIM in immune cell subsets, in the femoral marrow. This method retains the power to study molecular mechanisms underlying various cell functions in tissue context thus providing new insights into bone biology.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 4","pages":"1711-1731"},"PeriodicalIF":2.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12047734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introduction to the feature Issue \"Improving Vision through Intraocular Lenses\": a tribute to Jim Schwiegerling.","authors":"Susana Marcos, Pablo Artal, Damien Gatinel, Linda Lundström, Geunyoung Yoon, Nathan Lewis","doi":"10.1364/BOE.561537","DOIUrl":"10.1364/BOE.561537","url":null,"abstract":"<p><p>Cataract surgery, a transformative procedure to restore vision, has seen remarkable advancements in intraocular lens (IOL) technologies. This special issue presents a collection of research that explores the performance, design, and evaluation of IOLs. From established designs and the impact of key optical parameters to innovative approaches and preoperative simulations, these contributions offer a comprehensive view of current trends and future directions in IOL development. The special issue also honors the legacy of Prof. Jim Schwiegerling whose contributions to visual optics in general, and IOLs in particular, have had a tremendous impact in the field, both in the academic, clinical and industrial communities.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 4","pages":"1707-1710"},"PeriodicalIF":2.9,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12047719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiphoton microscopy at a microwatt level via gain-managed nonlinear amplification and pulse-picking.","authors":"Katarzyna Kunio, Grzegorz Soboń, Jakub Bogusławski","doi":"10.1364/BOE.557132","DOIUrl":"https://doi.org/10.1364/BOE.557132","url":null,"abstract":"<p><p>We introduce a compact, all-fiber laser system with a gain-managed nonlinear (GMN) amplified Yb:fiber oscillator and an integrated pulse-picker. The system delivers 39 fs pulses with peak powers of 0.83 MW and adjustable pulse repetition rates (0.3-15 MHz), enabling multiphoton imaging at remarkably low excitation powers (as low as 66 µW). Its design simplifies integration and enhances experimental flexibility. Compatible with two- and three-photon excitation, but also second harmonic generation microscopy, this versatile system offers precise control of imaging parameters, making it an effective tool for advancing multiphoton microscopy and other imaging techniques across various experimental environments.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 4","pages":"1692-1706"},"PeriodicalIF":2.9,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12047729/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}