Biomedical optics express最新文献_第8页

Visual acuity measured with a 3 mm pupil and Maxwellian view for subjective vs. objective focus in patients with exudative retinal disease. 用3mm瞳孔和麦克斯韦视场测量主观和客观焦点对渗出性视网膜疾病患者的视力。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-07-22 eCollection Date: 2026-08-01 DOI: 10.1364/BOE.603037

Ann E Elsner, Vamsi Parimi, Christopher A Clark, Brittany R Walker, Brett J King, Amani Fawzi

{"title":"Visual acuity measured with a 3 mm pupil and Maxwellian view for subjective vs. objective focus in patients with exudative retinal disease.","authors":"Ann E Elsner, Vamsi Parimi, Christopher A Clark, Brittany R Walker, Brett J King, Amani Fawzi","doi":"10.1364/BOE.603037","DOIUrl":"https://doi.org/10.1364/BOE.603037","url":null,"abstract":"We demonstrate a potential vision tester (PVT), designed to improve the accuracy of visual acuity (VA) measurements in older eyes with exudative retinal disease quantified by optical coherence tomography (OCT). Key features include a high-resolution visual display presented in Maxwellian view, a 3 mm pupil to limit wavefront (WF) aberrations, a selected fixed cylinder lens to correct astigmatism, and a tunable lens to correct sphere. An external Hartmann-Shack-based autorefractor provided objective sphere and cylinder corrections. Subjective values of the sphere were obtained by the patient adjusting the tunable lens. VA was measured using four alternative forced choice for a single black-on-white E stimulus. In patients with exudative retinal disease, VAs did not differ when using the objective vs. subjective sphere values. The significant difference in the myopic direction for two subjective settings (-0.093, -0.28 D) vs. one objective setting (0.0 D) was too small to cause a difference in VA for this patient group, supporting the use of an autorefractor for VA measurements.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 8","pages":"4296-4316"},"PeriodicalIF":3.2,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13481072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148787978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coherent modem-enabled spread-spectrum time-domain diffuse optical spectroscopy. 相干调制解调器支持扩频时域漫射光谱学。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-07-22 eCollection Date: 2026-08-01 DOI: 10.1364/BOE.601122

Jiaying Lu, Brandon Buscaino, Michael A Reimer, Mohammad E Mousa Pasandi, Kim Roberts

引用次数: 0

Fast volumetric imaging of a zebrafish seizure model with adaptive optics light sheet microscopy. 应用自适应光学薄片显微镜对斑马鱼癫痫模型进行快速体积成像。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-07-21 eCollection Date: 2026-08-01 DOI: 10.1364/BOE.596096

Bingxi Liu, Yang Liu, Carly Duffy, James D Lauderdale, Peter Kner

引用次数: 0

Generalized plaque digitization framework for multi-dimensional mesoscopic images. 多维介观图像的广义斑块数字化框架。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-07-20 eCollection Date: 2026-08-01 DOI: 10.1364/BOE.605322

Guixuan Gong, Xin Liu, Xueyan Jia, Ben Long, Siqi Chen, Tao Jiang, Yue Luo, Zhao Feng, Xiangning Li, Qingming Luo, Hui Gong, Anan Li

{"title":"Generalized plaque digitization framework for multi-dimensional mesoscopic images.","authors":"Guixuan Gong, Xin Liu, Xueyan Jia, Ben Long, Siqi Chen, Tao Jiang, Yue Luo, Zhao Feng, Xiangning Li, Qingming Luo, Hui Gong, Anan Li","doi":"10.1364/BOE.605322","DOIUrl":"https://doi.org/10.1364/BOE.605322","url":null,"abstract":"Fine analysis of the spatial distribution and morphology of Aβ plaques is crucial for understanding the pathological progression of Alzheimer's disease (AD). However, at the whole-brain scale, the enormous number of plaques, wide size range, diffuse morphologies, and complex imaging background pose challenges that existing digitization methods often fail to address comprehensively. To this end, this study developed a Generalized Plaque Digitization Framework (GPDigit). The framework adopts a two-step strategy of detection followed by segmentation: first, a customized object detection network achieves precise spatial localization of plaques; second, adaptive foreground signal segmentation is performed within local regions. This design circumvents the difficulty of global threshold selection and the high annotation cost of segmentation networks. GPDigit supports both 2D and 3D data scenarios, ensures detection accuracy through targeted feature extraction mechanisms, and significantly improves training data preparation efficiency with a self-developed annotation tool and multiple data augmentation strategies. Experimental results demonstrate that GPDigit achieves satisfactory digitization performance under challenging conditions such as dense plaque distribution, severe background interference, and weak signals. Application to whole-brain plaque analysis in 5xFAD mice across multiple key ages revealed, at the fine brain-region scale, the spatiotemporal heterogeneity of plaque density and load development. This study provides a systematic solution for plaque digitization in neuropathological mesoscopic high-resolution images and offers a practical tool to advance AD pathology research.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 8","pages":"4198-4215"},"PeriodicalIF":3.2,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13481076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148787867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Compact FPGA-based software correlator for real-time blood flow measurement with diffuse correlation spectroscopy. 用于漫射相关光谱实时血流测量的紧凑型fpga软件相关器。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-07-17 eCollection Date: 2026-08-01 DOI: 10.1364/BOE.601793

Vinh-Khanh Mai, Vishnukumar Raghu, Penaz Parveen Sultana Mohammad, Tristan Valenzuela, Ashwin B Parthasarathy

引用次数: 0

Magnetic resonance and X-ray contrast agents in the optical clearing of dermis - insights from ex vivo confocal Raman micro-spectroscopy. 磁共振和x射线造影剂在真皮光学清除中的应用——来自离体共聚焦拉曼显微光谱学的见解。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-07-17 eCollection Date: 2026-08-01 DOI: 10.1364/BOE.601566

Ali Jaafar, Bahaa Safaa, Maxim E Darvin, Tamás Váczi, Ahmed Al-Haddad, Valery V Tuchin, Miklós Veres

{"title":"Magnetic resonance and X-ray contrast agents in the optical clearing of dermis - insights from ex vivo confocal Raman micro-spectroscopy.","authors":"Ali Jaafar, Bahaa Safaa, Maxim E Darvin, Tamás Váczi, Ahmed Al-Haddad, Valery V Tuchin, Miklós Veres","doi":"10.1364/BOE.601566","DOIUrl":"https://doi.org/10.1364/BOE.601566","url":null,"abstract":"Confocal Raman micro-spectroscopy (CRM) has developed into a versatile tool that is frequently used to determine skin penetration of various compounds. Topically applied optical clearing agents decrease skin scattering, improving CRM imaging abilities. The use of magnetic resonance (MR) and X-ray contrast agents to increase transparency of biological objects has attracted more attention recently. By analysing Raman bands at 1003 and 1663 cm-1 and applying Fick's second law model, we calculated the diffusion coefficients of 3.51 × 10-8 cm2/s for Gadovist and 3.20 × 10-7 cm2/s for Omnipaque, with a slower diffusion regime for Dotarem. These diffusion kinetics directly governed penetration depth profiles across the investigated 0-240 µm dermal region and dictated optical clearing efficiency. Gadovist yielded the strongest short-term (30 min, up to 52% signal increase), whereas Omnipaque reached the highest and most depth-uniform optical clearing (60 min), consistent with its one-order-of-magnitude higher diffusivity. In contrast, Dotarem exhibited limited penetration and negligible optical clearing. Autofluorescence enhancement scaled with diffusion-driven refractive index matching, confirming that optical clearing is mechanistically coupled to molecular transport; it may also arise from protein structural modifications, oxidative stress, or increased protein density following contrast agent interaction. Together, these findings establish a quantitative link between contrast-agent diffusivity, dermal penetration depth, optical transparency, and tissue autofluorescence, providing a framework for integrating Raman, MR, and X-ray modalities in multimodal depth-resolved skin imaging.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 8","pages":"4159-4178"},"PeriodicalIF":3.2,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13481084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148787849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptive scan intervals in ultrawide-field OCT angiography achieved by a bidirectional interleaved approach. 双向交错方法实现超宽场OCT血管造影的自适应扫描间隔。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-07-16 eCollection Date: 2026-08-01 DOI: 10.1364/BOE.606139

Guangru B Liang, Shuibin Ni, Tristan T Hormel, Ringo Ng, Lauren Renner, Yukun Guo, Martha Neuringer, Yifan Jian, J Peter Campbell, Yali Jia

{"title":"Adaptive scan intervals in ultrawide-field OCT angiography achieved by a bidirectional interleaved approach.","authors":"Guangru B Liang, Shuibin Ni, Tristan T Hormel, Ringo Ng, Lauren Renner, Yukun Guo, Martha Neuringer, Yifan Jian, J Peter Campbell, Yali Jia","doi":"10.1364/BOE.606139","DOIUrl":"https://doi.org/10.1364/BOE.606139","url":null,"abstract":"Conventional unidirectional scanning rigidly couples the scan interval (t inter) to sampling density in ultra-wide field optical coherence tomography angiography (UWF-OCTA), which impedes optimal imaging of different blood flow velocities. In this study, we implement an adaptive bidirectional interleaved scanning pattern for UWF-OCTA that enables the adjustment of t inter while keeping the scan area, sampling density, and total acquisition time constant or optimally tuned. We implemented this pattern on our high-speed (800 kHz/1 MHz) swept-source UWF-OCTA prototypes and assessed image quality across several use cases, including pediatric patients, non-human primates, and rodents. This protocol successfully captured detailed UWF-OCTA images with well-defined microvascular patterns. We observed that, while keeping sampling density and total scan acquisition time constant, a longer t inter enhances sensitivity to slower retinal blood flow, whereas a shorter t inter yields more robust, motion-resistant images with clearer choroidal vasculature, as supported by quantitative contrast-to-noise ratio analysis. These findings illustrate that the optimal t inter is dependent on the specific application and pathology, and validate the adaptive scanning pattern as a useful approach for tailoring UWF-OCTA to diverse clinical needs.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 8","pages":"4145-4158"},"PeriodicalIF":3.2,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13481069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148787936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wavelength-tunable depletion expands fluorophore compatibility in two-photon STED nanoscopy. 波长可调耗尽扩展荧光团相容性在双光子STED纳米显微镜。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-07-16 eCollection Date: 2026-08-01 DOI: 10.1364/BOE.603553

Joe Sakamoto, Hirokazu Ishii, Kohei Otomo, Yuichi Kozawa, Shunichi Sato, Hiroyuki Yokoyama, Tomomi Nemoto

{"title":"Wavelength-tunable depletion expands fluorophore compatibility in two-photon STED nanoscopy.","authors":"Joe Sakamoto, Hirokazu Ishii, Kohei Otomo, Yuichi Kozawa, Shunichi Sato, Hiroyuki Yokoyama, Tomomi Nemoto","doi":"10.1364/BOE.603553","DOIUrl":"https://doi.org/10.1364/BOE.603553","url":null,"abstract":"We report the development of a novel two-photon excitation stimulated emission depletion microscopy system, namely 2PE-scSTED, featuring a custom light source setup that integrates a supercontinuum (SC) laser light source with our proprietary 1064 nm pulsed semiconductor excitation laser. This SC source provides the capability of emitting a broadband spectral range, enabling highly reliable selection of depletion wavelength. Utilizing 2PE-scSTED, we investigated the depletion characteristics of several fluorophores. By employing a tunable filter to precisely select depletion wavelength from 640 to 800 nm, we systematically measured the depletion efficiency and identified the optimal wavelengths that maximize fluorescence depletion efficiency for four distinct fluorescent probes. This approach yielded super-resolution images with a lateral spatial resolution as fine as 45 nm (approximately λ 2PE /24) with a relatively lower depletion power than our previous reports, and can be principally applicable to all fluorescent probes. Our results demonstrate that the integration of a broadband SC source removes the spectral constraint of fluorescence depletion, enabling tailored 2PE-STED microscopy for individual fluorophores. This enhances the versatility and applicability of 2PE-STED microscopy for super-resolution imaging of biological specimens.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 8","pages":"4131-4144"},"PeriodicalIF":3.2,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13481070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum: Physiological chromatic model eyes for IOL characterization: erratum. 校正：人工晶状体表征的生理色度模型眼：校正。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-07-10 eCollection Date: 2026-08-01 DOI: 10.1364/BOE.609207

Miguel Faria-Ribeiro, Noé Villemagne, Luc Joannes

引用次数: 0

Evaluation of Shack-Hartmann wavefront sensing artifacts due to reflectivity variations across thick layered samples using the discrete Fourier transform. 利用离散傅里叶变换评估厚层样品反射率变化引起的Shack-Hartmann波前传感伪影。

IF 3.2 2区医学

Biomedical optics express Pub Date : 2026-07-10 eCollection Date: 2026-08-01 DOI: 10.1364/BOE.596303

Paresh Kumar Sahoo, Arjun Raj M R, Vyas Akondi

{"title":"Evaluation of Shack-Hartmann wavefront sensing artifacts due to reflectivity variations across thick layered samples using the discrete Fourier transform.","authors":"Paresh Kumar Sahoo, Arjun Raj M R, Vyas Akondi","doi":"10.1364/BOE.596303","DOIUrl":"https://doi.org/10.1364/BOE.596303","url":null,"abstract":"Multiple wavefronts originating from axially and/or transversely displaced light sources are known to introduce artifactual aberrations in Shack-Hartmann wavefront sensors (SHWSs). These aberrations are primarily dominated by defocus, followed by spherical aberration and coma, depending on whether an on-axis or off-axis binary transmission pupil mask is used in the illumination path. In an earlier study, such artifactual aberrations-caused by multiple reflections from displaced sources-were predicted using rigorous diffraction-theory-based integral calculations. However, such calculations are computationally intensive, and were therefore limited to modeling only two reflecting layers. Moreover, the low Fresnel number associated with SHWS lenslets introduces a focal shift, complicating the application of the discrete Fourier transform (DFT). Here, we showed that, with an appropriate coordinate transformation, the diffraction integrals that account for focal shift can be expressed as Fourier transform integrals. Using this approach to model human retinal reflectivity from 75 axially separated layers, we demonstrate a computational speed-up of over three orders of magnitude. Our results reveal that wavefront error in multilayered structures is fundamentally sensitive to both retinal architecture and centroiding strategy. While dynamic beacon positioning remains a primary mitigation strategy, small on-axis illumination paired with polarizers offers a robust alternative for substantially minimizing these artifactual aberrations. These findings have broad implications for optical systems requiring precise wavefront sensing in volumetric samples, including microscopy, retinal imaging, and astronomy.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 8","pages":"4115-4130"},"PeriodicalIF":3.2,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13481079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148787802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0