Biomedical optics express最新文献_第7页

Line-scan chromatic confocal microscopy for multi-depth imaging of skin tissue using deconvolution. 线扫描彩色共聚焦显微镜用于皮肤组织的多深度成像使用反褶积。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-05-29 eCollection Date: 2025-06-01 DOI: 10.1364/BOE.562118

Wenhao Zhao, YongKang Liang, Rui Shang, Kun Yue, Wenping Guo, Min Xia

{"title":"Line-scan chromatic confocal microscopy for multi-depth imaging of skin tissue using deconvolution.","authors":"Wenhao Zhao, YongKang Liang, Rui Shang, Kun Yue, Wenping Guo, Min Xia","doi":"10.1364/BOE.562118","DOIUrl":"10.1364/BOE.562118","url":null,"abstract":"In this paper, we present a line-scan chromatic confocal microscopy for rapid, multi-depth imaging of non-transparent skin tissue. Leveraging spectral dispersion encoding, this system enables the simultaneous acquisition of reflectance data across a depth range exceeding 180 <math><mrow><mi>μ</mi> </mrow> </math> m in skin tissue, effectively eliminating mechanical axial scanning and minimizing associated artifacts. With a lateral effective field of view (FOV) of 13.2 mm, the system provides a wide-field perspective for comprehensive tissue analysis. Furthermore, to overcome the typical limitations of lateral resolution in line-scan systems, we employ pre-calibrated deconvolution using an empirically determined point spread function obtained with a micro-spot mirror. We demonstrate deep-tissue tomography with ex vivo bovine skin tissue, achieving an increase in resolution from 45.25 lp/mm to 50.79 lp/mm post-deconvolution, as quantified using a USAF 1951 target. These results highlight the potential of line-scan chromatic confocal microscopy with pre-calibrated deconvolution as a powerful platform for multi-depth biomedical imaging applications requiring enhanced lateral resolution and contrast.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 6","pages":"2516-2527"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tartrazine-enabled optical clearing for in vivo optical resolution photoacoustic microscopy. 用于体内光学分辨率光声显微镜的酒石黄启用光学清除。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-05-29 eCollection Date: 2025-06-01 DOI: 10.1364/BOE.565643

Conger Jia, Zhiling Zhang, Yuecheng Shen, Wanli Hou, Jiayu Zhao, Jiawei Luo, Haoran Chen, Dalong Qi, Yunhua Yao, Lianzhong Deng, Hongmei Ma, Zhenrong Sun, Shian Zhang

{"title":"Tartrazine-enabled optical clearing for in vivo optical resolution photoacoustic microscopy.","authors":"Conger Jia, Zhiling Zhang, Yuecheng Shen, Wanli Hou, Jiayu Zhao, Jiawei Luo, Haoran Chen, Dalong Qi, Yunhua Yao, Lianzhong Deng, Hongmei Ma, Zhenrong Sun, Shian Zhang","doi":"10.1364/BOE.565643","DOIUrl":"10.1364/BOE.565643","url":null,"abstract":"Optical-resolution photoacoustic microscopy (OR-PAM) is a powerful imaging technique that visualizes microvascular and tissue structures with high spatial resolution, offering valuable insights into physiological and pathological processes. However, strong optical scattering in biological tissues fundamentally limits its imaging depth. Recent studies have suggested that tartrazine, a food-grade water-soluble dye, may serve as an effective optical clearing agent (OCA), yet its strong optical absorption near 532 nm raises concerns about compatibility with green-light-based OR-PAM systems. In this study, we demonstrate that by carefully controlling the concentration of tartrazine, it can effectively function as an OCA for OR-PAM at 532 nm without compromising signal quality. In vivo experiments on mouse ear and abdominal tissue showed that tartrazine significantly enhanced microvascular visibility across all tested concentrations, with the 15% weight of solute per weight of solution providing optimal clearing performance. Additionally, we evaluated 4-aminoantipyrine as another dye-based OCA, which also improved image clarity, albeit to a lesser extent. These findings highlight the promise of absorbing dye-based OCAs like tartrazine in enhancing in vivo OR-PAM by mitigating light scattering, potentially enabling deeper and clearer photoacoustic imaging in biomedical applications.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 6","pages":"2504-2515"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vignetting and peripheral double images with intraocular lenses. 人工晶状体的渐晕和周边双像。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-05-29 eCollection Date: 2025-06-01 DOI: 10.1364/BOE.559745

Michael J Simpson, David L Cooke, Maria Muzyka-Woźniak

引用次数: 0

Development of a multispectral imaging apparatus for cost-effective fundus disease detection. 一种具有成本效益的眼底疾病检测多光谱成像设备的研制。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-05-28 eCollection Date: 2025-06-01 DOI: 10.1364/BOE.563950

Yingchao Shi, Luming Zhang, Xin Shu, Keke Zhang, Yuchao Yan, Weizheng Yuan, Yiting Yu, Yan Gong

{"title":"Development of a multispectral imaging apparatus for cost-effective fundus disease detection.","authors":"Yingchao Shi, Luming Zhang, Xin Shu, Keke Zhang, Yuchao Yan, Weizheng Yuan, Yiting Yu, Yan Gong","doi":"10.1364/BOE.563950","DOIUrl":"10.1364/BOE.563950","url":null,"abstract":"Fundus spectral imaging (FSI) integrates fundus photography with spectral techniques, providing both spatial and spectral information for retinal imaging. Whereas existing FSI systems have demonstrated advantages in structural and functional imaging, their widespread adoption is hindered by high costs and complex optical designs. To address these challenges, we propose a low-cost multispectral fundus camera with a simplified optical design, built from off-the-shelf optics, 3D-printed parts, and equipped with fiber-bundle-coupled multi-wavelength LED illumination source (470-740 nm). Additionally, the proposed multispectral imaging apparatus incorporates a coaxial non-separated polarization-based reflection suppression technique, using orthogonal polarizers to suppress corneal reflections without pupil-plane separation. To the best of our knowledge, this is the first application of such an architecture in the context of FSI. Experimental results demonstrate that the developed system achieves high-quality FSI under low-cost conditions, validating its feasibility as a practical solution. Clinical validation validates its diagnostic capability for diabetic retinopathy, choroidal pigmented nevus, and, notably, the first reported spectral imaging of peripapillary atrophy. The system achieves performance comparable to conventional color fundus photography while enabling superior diagnosis of deep fundus conditions such as choroidal lesions, offering a cost-effective and practical FSI solution for broader deployment in resource-limited settings.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 6","pages":"2482-2494"},"PeriodicalIF":2.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SAFFusion: a saliency-aware frequency fusion network for multimodal medical image fusion. SAFFusion：一种用于多模态医学图像融合的显著性感知频率融合网络。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-05-27 eCollection Date: 2025-06-01 DOI: 10.1364/BOE.555458

Renhe Liu, Yu Liu, Han Wang, Junxian Li, Kai Hu

{"title":"SAFFusion: a saliency-aware frequency fusion network for multimodal medical image fusion.","authors":"Renhe Liu, Yu Liu, Han Wang, Junxian Li, Kai Hu","doi":"10.1364/BOE.555458","DOIUrl":"10.1364/BOE.555458","url":null,"abstract":"Medical image fusion integrates complementary information from multimodal medical images to provide comprehensive references for clinical decision-making, such as the diagnosis of Alzheimer's disease and the detection and segmentation of brain tumors. Although traditional and deep learning-based fusion methods have been extensively studied, they often fail to devise targeted strategies that fully utilize distinct regional or feature-specific information. This paper proposes SAFFusion, a saliency-aware frequency fusion network that integrates intensity and texture cues from multimodal medical images. We first introduce Mamba-UNet, a multiscale encoder-decoder architecture enhanced by the Mamba design, to improve global modeling in feature extraction and image reconstruction. By employing the contourlet transform in Mamba-UNet, we replace conventional pooling with multiscale representations and decompose spatial features into high- and low-frequency subbands. A dual-branch frequency feature fusion module then fuses cross-modality information according to the distinct characteristics of these frequency subbands. Furthermore, we apply latent low-rank representation (LatLRR) to assess image saliency and implement adaptive loss constraints to preserve information in salient and non-salient regions. Quantitative results on CT/MRI, SPECT/MRI, and PET/MRI fusion tasks show that SAFFusion outperforms state-of-the-art methods. Qualitative evaluations confirm that SAFFusion effectively merges prominent intensity features and rich textures from multiple sources.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 6","pages":"2459-2481"},"PeriodicalIF":2.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic presbyopia correction in the macular field of view by using a liquid crystal lens. 动态老花眼矫正黄斑视野的液晶透镜。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-05-21 eCollection Date: 2025-06-01 DOI: 10.1364/BOE.557747

Louis Bégel, Tigran Galstian

引用次数: 0

Research on an all-medium two-parameter metasurface sensor based on Fano resonance. 基于范诺共振的全介质双参数超表面传感器研究。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-05-20 eCollection Date: 2025-06-01 DOI: 10.1364/BOE.562157

Xin Zhang, Jiguo Li, Chao Liu, Huansong Huang, Minghui Gu, Sijia Jiang, Qingbin Jiao, Ding Ma, Mingyu Yang, Liang Xu, Xin Tan

引用次数: 0

High-resolution multimodal visible light optical coherence tomography and scanning laser ophthalmoscopy for in vivo neuronal and vascular retinal imaging in mice. 高分辨率多模态可见光光学相干断层扫描和扫描激光检眼镜用于小鼠体内神经元和血管视网膜成像。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-05-19 eCollection Date: 2025-06-01 DOI: 10.1364/BOE.560539

Siyu Song, Guangru Ben Liang, Tristan T Hormel, Yukun Guo, Min Gao, Benjamin Sivyer, J Peter Campbell, Siyu Chen, Yifan Jian, Yali Jia

引用次数: 0

Power spectrum curve compensation method for image quality enhancement in high-throughput gene sequencing. 高通量基因测序图像质量增强的功率谱曲线补偿方法。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-05-15 eCollection Date: 2025-06-01 DOI: 10.1364/BOE.562958

Jianglan Wang, Siyuan Yao, Suocheng Duan, Yu Jiao, Xin Zhang, Xindong Chen

{"title":"Power spectrum curve compensation method for image quality enhancement in high-throughput gene sequencing.","authors":"Jianglan Wang, Siyuan Yao, Suocheng Duan, Yu Jiao, Xin Zhang, Xindong Chen","doi":"10.1364/BOE.562958","DOIUrl":"10.1364/BOE.562958","url":null,"abstract":"In high-throughput gene sequencing, the quality of sequencing images is critical for the accuracy of subsequent base calling. However, during practical sequencing processes, the time delay integration (TDI) camera's push-scan imaging often leads to significant degradation of image quality along the push-scan direction. Addressing the current limitations in TDI image restoration research for gene sequencing, this study establishes an imaging spectrum model of sequencing images based on MGI's ultra-high-throughput sequencer. We systematically analyze the causes and intrinsic mechanisms of image quality degradation, with a focus on elucidating the specific impacts of TDI push-scanning on image quality. To enhance TDI sequencing image quality, we compare the differences in power spectral projection curves between stare-mode imaging and TDI push-scan imaging and propose a power spectrum curve compensation (PSCC)-based quality optimization method alongside a novel evaluation framework for sequencing image quality. Experimental results demonstrate that compared to original H-channel images from cycle 1 to 50, the energy concentration (1/σ) of the optimized images increases by 9.13% in the TDI direction and 4.64% in the direction perpendicular to TDI. Signal-to-noise ratio (SNR) increases by 6.90% for base A and 4.99% for base C, while base calling accuracy (Q30) improves by 1.67%.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 6","pages":"2351-2364"},"PeriodicalIF":2.9,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two-photon microscopy using picosecond pulses from four-wave mixing in a Yb-doped photonic crystal fiber. 利用掺镱光子晶体光纤中四波混频产生的皮秒脉冲的双光子显微镜。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-05-14 eCollection Date: 2025-06-01 DOI: 10.1364/BOE.563581

Bartosz Krawczyk, Alexandre Kudlinski, Robert T Murray, Simon R Schultz, Amanda J Foust, Timothy H Runcorn

{"title":"Two-photon microscopy using picosecond pulses from four-wave mixing in a Yb-doped photonic crystal fiber.","authors":"Bartosz Krawczyk, Alexandre Kudlinski, Robert T Murray, Simon R Schultz, Amanda J Foust, Timothy H Runcorn","doi":"10.1364/BOE.563581","DOIUrl":"10.1364/BOE.563581","url":null,"abstract":"Two-photon microscopy (TPM) enables deep tissue imaging but requires excitation pulses that have a large product of average and peak power, typically supplied by femtosecond solid-state lasers. However, these lasers are bulky, and femtosecond pulses require careful dispersion management to avoid pulse broadening, particularly when delivery fibers are used. Here we present a compact, fiber-based picosecond laser source operating at 790 nm for TPM using an ytterbium-doped photonic crystal fiber (Yb-doped PCF). The Yb-doped PCF simultaneously amplifies 1064 nm input pulses and efficiently converts them to 790 nm via four-wave mixing, generating pulses with a peak power of up to ∼3.8 kW. The source has a variable repetition rate (1.48 MHz-14.78 MHz), enabling the two-photon excitation fluorescence signal to be maximized in the presence of excitation saturation. We benchmark our picosecond laser source against a femtosecond Ti:Sapphire laser for TPM of stained Convallaria majalis samples and demonstrate comparable fluorescence signal when the two-photon excitation conditions are matched.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 6","pages":"2327-2336"},"PeriodicalIF":2.9,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0