High-resolution multimodal visible light optical coherence tomography and scanning laser ophthalmoscopy for in vivo neuronal and vascular retinal imaging in mice.

IF 3.2 2区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Biomedical optics express Pub Date : 2025-05-19 eCollection Date: 2025-06-01 DOI:10.1364/BOE.560539

Siyu Song, Guangru Ben Liang, Tristan T Hormel, Yukun Guo, Min Gao, Benjamin Sivyer, J Peter Campbell, Siyu Chen, Yifan Jian, Yali Jia

{"title":"High-resolution multimodal visible light optical coherence tomography and scanning laser ophthalmoscopy for in vivo neuronal and vascular retinal imaging in mice.","authors":"Siyu Song, Guangru Ben Liang, Tristan T Hormel, Yukun Guo, Min Gao, Benjamin Sivyer, J Peter Campbell, Siyu Chen, Yifan Jian, Yali Jia","doi":"10.1364/BOE.560539","DOIUrl":null,"url":null,"abstract":"Microglial cells play a crucial role in retinal vascular and brain diseases through complex interactions with blood vessels and neurons. To image retinal structures, vasculature, and microglia, we developed a multimodal system integrating visible light optical coherence tomography (vis-OCT) and scanning laser ophthalmoscopy (SLO). Both subsystems achieve micron-scale resolutions and operate within the diffraction limit across a 34-degree field of view: theoretically, the OCT system offers an axial resolution of 2.12 μm and a transverse resolution of 8.78 μm, while the SLO system provides a transverse resolution of 7.1 μm. We validated the system performance using transgenic mice with fluorescent protein-labeled microglia, revealing detailed retinal microstructures, microvasculature, and individual microglia with distinguishable branches, confirmed by ex vivo microscopy.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 6","pages":"2365-2375"},"PeriodicalIF":3.2000,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265451/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical optics express","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1364/BOE.560539","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

Microglial cells play a crucial role in retinal vascular and brain diseases through complex interactions with blood vessels and neurons. To image retinal structures, vasculature, and microglia, we developed a multimodal system integrating visible light optical coherence tomography (vis-OCT) and scanning laser ophthalmoscopy (SLO). Both subsystems achieve micron-scale resolutions and operate within the diffraction limit across a 34-degree field of view: theoretically, the OCT system offers an axial resolution of 2.12 μm and a transverse resolution of 8.78 μm, while the SLO system provides a transverse resolution of 7.1 μm. We validated the system performance using transgenic mice with fluorescent protein-labeled microglia, revealing detailed retinal microstructures, microvasculature, and individual microglia with distinguishable branches, confirmed by ex vivo microscopy.

查看原文本刊更多论文

高分辨率多模态可见光光学相干断层扫描和扫描激光检眼镜用于小鼠体内神经元和血管视网膜成像。

小胶质细胞通过与血管和神经元的复杂相互作用，在视网膜血管和脑部疾病中起着至关重要的作用。为了成像视网膜结构、血管系统和小胶质细胞，我们开发了一种多模态系统，集成了可见光光学相干断层扫描（vis-OCT）和扫描激光眼科检查（SLO）。两个子系统都实现了微米级的分辨率，并在34度视场的衍射极限内运行：理论上，OCT系统提供2.12 μm的轴向分辨率和8.78 μm的横向分辨率，而SLO系统提供7.1 μm的横向分辨率。我们用带有荧光蛋白标记的小胶质细胞的转基因小鼠验证了该系统的性能，揭示了详细的视网膜微结构、微血管和具有可区分分支的单个小胶质细胞，并通过离体显微镜证实了这一点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biomedical optics express BIOCHEMICAL RESEARCH METHODS-OPTICS

CiteScore

6.80

自引率

11.80%

发文量

633

审稿时长

1 months

期刊介绍： The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including: Tissue optics and spectroscopy Novel microscopies Optical coherence tomography Diffuse and fluorescence tomography Photoacoustic and multimodal imaging Molecular imaging and therapies Nanophotonic biosensing Optical biophysics/photobiology Microfluidic optical devices Vision research.