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Biomedical optics express最新文献

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Rapid, sensitive and multiplexed detection of SARS-CoV-2 viral nucleic acids enabled by phase-based surface plasmon resonance of metallic gratings. 利用金属光栅的相位表面等离子体共振技术实现对 SARS-CoV-2 病毒核酸的快速、灵敏和多重检测。
IF 3.4 2区 医学
Biomedical optics express Pub Date : 2024-08-13 DOI: 10.1364/boe.535051
Shuai Sun,Yuanyuan Zhao,Yuxuan Hao,Pan Xue,Xiaoyang Guo,Wei Zhang,Chunxiang Zhang,Qiongdi Zhang,Wenhua Zhou,Hock Chun Ong,Xuefeng Yu,Zeren Li,Jia Li,Jianquan Yao
{"title":"Rapid, sensitive and multiplexed detection of SARS-CoV-2 viral nucleic acids enabled by phase-based surface plasmon resonance of metallic gratings.","authors":"Shuai Sun,Yuanyuan Zhao,Yuxuan Hao,Pan Xue,Xiaoyang Guo,Wei Zhang,Chunxiang Zhang,Qiongdi Zhang,Wenhua Zhou,Hock Chun Ong,Xuefeng Yu,Zeren Li,Jia Li,Jianquan Yao","doi":"10.1364/boe.535051","DOIUrl":"https://doi.org/10.1364/boe.535051","url":null,"abstract":"A rapid, sensitive and easy-to-implement approach is proposed for the detection of pathogenic nucleic acids based on phase-based plasmonic spectroscopy of metallic gratings. The plasmonic sensors were fabricated using interference lithography and functionalized with single-stranded DNA probes to specific target SARS-CoV-2. The biosensor achieved the detection of 40 fM viral nucleic acids within 5 min; furthermore, a detection capability of 1 aM (0.6 copies/µL) was acquired when combining with the recombinase polymerase amplification. Additionally, the multiplexed sensing system was demonstrated to simultaneously detect three genomic sequences on a single sensor chip, thereby enhancing diagnostic accuracy and enabling high-throughput detection.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of vinculin tension in cellular monolayers and three-dimensional multicellular aggregates. 细胞单层和三维多细胞聚集体中长春花素张力的比较。
IF 3.4 2区 医学
Biomedical optics express Pub Date : 2024-08-13 DOI: 10.1364/boe.529156
Luni Hu,Rick I Cohen,Margarida Barroso,Nada N Boustany
{"title":"Comparison of vinculin tension in cellular monolayers and three-dimensional multicellular aggregates.","authors":"Luni Hu,Rick I Cohen,Margarida Barroso,Nada N Boustany","doi":"10.1364/boe.529156","DOIUrl":"https://doi.org/10.1364/boe.529156","url":null,"abstract":"Confocal frequency-domain fluorescence lifetime and Förster resonance energy transfer (FRET) microscopy of Chinese hamster ovary (CHO-K1) cells expressing the vinculin tension sensor (VinTS) is used to compare vinculin tension in three-dimensional (3D) multicellular aggregates and 2D cellular monolayers. In both 2D and 3D cultures, the FRET efficiency of VinTS is 5-6% lower than that of VinTL (p < 0.05), a tail-less control which cannot bind actin or paxillin. The difference between VinTS and VinTL FRET efficiency can be mitigated by treatment with the Rho-associated kinase inhibitor Y-27632, demonstrating that VinTS is under tension in both 2D and 3D cultures. However, there is an overall decrease in FRET efficiency of both VinTS and VinTL in the 3D multicellular aggregates compared with the 2D monolayers. Expression of VinTS in 2D and 3D cultures exhibits puncta consistent with cellular adhesions. While paxillin is present at the sites of VinTS expression in the 2D monolayers, it is generally absent from VinTS puncta in the 3D aggregates. The results suggest that VinTS experiences a modified environment in 3D aggregates compared with 2D monolayers and provide a basis for further investigation of molecular tension sensors in 3D tissue models.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SODL-IR-FISTA: sparse online dictionary learning with iterative reduction FISTA for cone-beam X-ray luminescence computed tomography. SODL-IR-FISTA：用于锥束 X 射线发光计算机断层扫描的稀疏在线词典学习与迭代还原 FISTA。
IF 3.4 2区 医学
Biomedical optics express Pub Date : 2024-08-12 DOI: 10.1364/boe.531828
Xin Cao,Wenlong Tang,Huimin Gao,Yifan Wang,Yi Chen,Chengyi Gao,Fengjun Zhao,Linzhi Su
{"title":"SODL-IR-FISTA: sparse online dictionary learning with iterative reduction FISTA for cone-beam X-ray luminescence computed tomography.","authors":"Xin Cao,Wenlong Tang,Huimin Gao,Yifan Wang,Yi Chen,Chengyi Gao,Fengjun Zhao,Linzhi Su","doi":"10.1364/boe.531828","DOIUrl":"https://doi.org/10.1364/boe.531828","url":null,"abstract":"Cone beam X-ray luminescence computed tomography (CB-XLCT) is an emerging imaging technique with potential for early 3D tumor detection. However, the reconstruction challenge due to low light absorption and high scattering in tissues makes it a difficult inverse problem. In this study, the online dictionary learning (ODL) method, combined with iterative reduction FISTA (IR-FISTA), has been utilized to achieve high-quality reconstruction. Our method integrates IR-FISTA for efficient and accurate sparse coding, followed by an online stochastic approximation for dictionary updates, effectively capturing the sparse features inherent to the problem. Additionally, a re-sparse step is introduced to enhance the sparsity of the solution, making it better suited for CB-XLCT reconstruction. Numerical simulations and in vivo experiments were conducted to assess the performance of the method. The SODL-IR-FISTA achieved the smallest location error of 0.325 mm in in vivo experiments, which is 58% and 45% of the IVTCG-L 1 (0.562 mm) and OMP-L 0 (0.721 mm), respectively. Additionally, it has the highest DICE similarity coefficient, which is 0.748. The results demonstrate that our approach outperforms traditional methods in terms of localization precision, shape restoration, robustness, and practicality in live subjects.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of the international standard for functional near-infrared spectroscopy as a medical equipment 制定作为医疗设备的功能性近红外光谱国际标准
IF 2.9 2区 医学
Biomedical optics express Pub Date : 2024-08-09 DOI: 10.1364/boe.531664
Hiroshi Kawaguchi, Hideo Eda
{"title":"Development of the international standard for functional near-infrared spectroscopy as a medical equipment","authors":"Hiroshi Kawaguchi, Hideo Eda","doi":"10.1364/boe.531664","DOIUrl":"https://doi.org/10.1364/boe.531664","url":null,"abstract":"","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141921984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A naturalistic approach to investigate the neural correlates of a laundry cycle with and without fragrance 用自然方法研究有香味和无香味洗衣周期的神经相关性
IF 2.9 2区 医学
Biomedical optics express Pub Date : 2024-08-09 DOI: 10.1364/boe.528275
Giuliano Gaeta, N. Gunasekara, P. Pinti, Andrew Levy, Emilia Parkkinen, Emily Kontaris, I. Tachtsidis
{"title":"A naturalistic approach to investigate the neural correlates of a laundry cycle with and without fragrance","authors":"Giuliano Gaeta, N. Gunasekara, P. Pinti, Andrew Levy, Emilia Parkkinen, Emily Kontaris, I. Tachtsidis","doi":"10.1364/boe.528275","DOIUrl":"https://doi.org/10.1364/boe.528275","url":null,"abstract":"","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141923824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Through-focus performance and off-axis effects in aspheric monofocal intraocular lenses 非球面单焦点眼内透镜的通焦性能和离轴效应
IF 2.9 2区 医学
Biomedical optics express Pub Date : 2024-08-09 DOI: 10.1364/boe.533714
Grzegorz Labuz, Weijia Yan, R. Khoramnia, G. Auffarth
{"title":"Through-focus performance and off-axis effects in aspheric monofocal intraocular lenses","authors":"Grzegorz Labuz, Weijia Yan, R. Khoramnia, G. Auffarth","doi":"10.1364/boe.533714","DOIUrl":"https://doi.org/10.1364/boe.533714","url":null,"abstract":"","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141921439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Adversarial training collaborating hybrid convolution-transformer network for automatic identification of reactive lymphocytes in peripheral blood. 用于自动识别外周血中反应性淋巴细胞的对抗训练协作混合卷积-变换器网络。
IF 3.4 2区 医学
Biomedical optics express Pub Date : 2024-08-09 DOI: 10.1364/boe.525119
Liye Mei,Haoran Peng,Ping Luo,Shuangtong Jin,Hui Shen,Jing He,Wei Yang,Zhiwei Ye,Haigang Sui,Mengqing Mei,Cheng Lei,Bei Xiong
{"title":"Adversarial training collaborating hybrid convolution-transformer network for automatic identification of reactive lymphocytes in peripheral blood.","authors":"Liye Mei,Haoran Peng,Ping Luo,Shuangtong Jin,Hui Shen,Jing He,Wei Yang,Zhiwei Ye,Haigang Sui,Mengqing Mei,Cheng Lei,Bei Xiong","doi":"10.1364/boe.525119","DOIUrl":"https://doi.org/10.1364/boe.525119","url":null,"abstract":"Reactive lymphocytes may indicate diseases such as viral infections. Identifying these abnormal lymphocytes is crucial for disease diagnosis. Currently, reactive lymphocytes are mainly manually identified by pathological experts with microscopes and morphological knowledge, which is time-consuming and laborious. Some studies have used convolutional neural networks (CNNs) to identify peripheral blood leukocytes, but there are limitations in the small receptive field of the model. Our model introduces a transformer based on CNN, expands the receptive field of the model, and enables it to extract global features more efficiently. We also enhance the generalization ability of the model through virtual adversarial training (VAT) without changing the parameters of the model. Finally, our model achieves an overall accuracy of 93.66% on the test set, and the accuracy of reactive lymphocytes also reaches 88.03%. This work takes another step toward the efficient identification of reactive lymphocytes.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optical directional differential operation enabled visual chirality detection 实现视觉手性检测的光学定向差分操作
IF 2.9 2区 医学
Biomedical optics express Pub Date : 2024-08-08 DOI: 10.1364/boe.531056
Yong Zhang, Minghe Wang, Ting Jiang, Zhaoxin Wen, Jiaxin Xiao, Zhao Luo
{"title":"Optical directional differential operation enabled visual chirality detection","authors":"Yong Zhang, Minghe Wang, Ting Jiang, Zhaoxin Wen, Jiaxin Xiao, Zhao Luo","doi":"10.1364/boe.531056","DOIUrl":"https://doi.org/10.1364/boe.531056","url":null,"abstract":"","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141927149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chromatic cues for the sign of defocus in the peripheral retina. 周边视网膜散焦迹象的色度线索。
IF 3.4 2区 医学
Biomedical optics express Pub Date : 2024-08-08 DOI: 10.1364/boe.537268
Len Zheleznyak,Chang Liu,Simon Winter
{"title":"Chromatic cues for the sign of defocus in the peripheral retina.","authors":"Len Zheleznyak,Chang Liu,Simon Winter","doi":"10.1364/boe.537268","DOIUrl":"https://doi.org/10.1364/boe.537268","url":null,"abstract":"Detecting optical defocus at the retina is crucial for accurate accommodation and emmetropization. However, the optical characteristics of ocular defocus are not fully understood. To bridge this knowledge gap, we simulated polychromatic retinal image quality by considering both the monochromatic wavefront aberrations and chromatic aberrations of the eye, both in the fovea and the periphery (nasal visual field). Our study revealed two main findings: (1) chromatic and monochromatic aberrations interact to provide a signal to the retina (chromatic optical anisotropy) to discern positive from negative defocus and (2) that chromatic optical anisotropy exhibited notable differences among refractive error groups (myopes, emmetropes and hyperopes). These findings could enhance our understanding of the underlying mechanisms of defocus detection and their subsequent implications for myopia control therapies. Further research is needed to explore the retinal architecture's ability to utilize the optical signals identified in this study.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Longitudinal tracking of perfluorooctanoic acid exposure on mammary epithelial cell spheroids by dynamic optical coherence tomography. 通过动态光学相干断层扫描纵向追踪乳腺上皮细胞球体接触全氟辛酸的情况。
IF 3.4 2区 医学
Biomedical optics express Pub Date : 2024-08-08 DOI: 10.1364/boe.530775
Lin Yang,Pan Ji,Abel A Miranda Buzetta,Haolin Li,Matthew R Lockett,Haibo Zhou,Amy L Oldenburg
{"title":"Longitudinal tracking of perfluorooctanoic acid exposure on mammary epithelial cell spheroids by dynamic optical coherence tomography.","authors":"Lin Yang,Pan Ji,Abel A Miranda Buzetta,Haolin Li,Matthew R Lockett,Haibo Zhou,Amy L Oldenburg","doi":"10.1364/boe.530775","DOIUrl":"https://doi.org/10.1364/boe.530775","url":null,"abstract":"We investigated the morphology and intracellular motility of mammary epithelial cell (MCF10DCIS.com) spheroids cultured in 3D artificial extracellular matrix under perfluorooctanoic acid (PFOA) exposure. Dynamic optical coherence tomography (OCT) was employed for real-time, non-invasive imaging of these spheroids longitudinally over 12 days under PFOA exposures up to 500 µM. Despite no significant changes in volume or asphericity of spheroids, morphological alterations were observed in OCT images of spheroids at 100 µM on Day 12 and from Day 4 at 500 µM. Intracellular motility was assessed by the inverse-power-law exponent of the speckle fluctuation spectrum (α), and an autocorrelation-based motility amplitude (M). Linear regression indicated that both PFOA concentration and culture time are highly significant predictors for both α and M (p < 0.001 for all). Both PFOA concentration and culture time have positive associations with α and negative association with M, where increased α indicates suppression of higher frequency fluctuations (∼> 2 Hz) relative to those at lower frequencies, and decreased M indicates overall suppression of intracellular motility. This study can lead to the future development of biomarkers for per- and polyfluoroalkyl substances (PFAS) exposure using dynamic OCT and its associated toolkit of quantitative metrics.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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