Asymmetric activation of retinal ON and OFF pathways by AOSLO raster-scanned visual stimuli.

IF 3.2 2区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Biomedical optics express Pub Date : 2025-06-09 eCollection Date: 2025-07-01 DOI:10.1364/BOE.566008

Sara S Patterson, Yongyi Cai, Qiang Yang, William H Merigan, David R Williams

引用次数: 0

Abstract

Adaptive optics scanning light ophthalmoscopy (AOSLO) enables high-resolution retinal imaging, eye tracking, and stimulus delivery in the living eye. AOSLO-mediated visual stimuli are created by temporally modulating the excitation light as it scans across the retina. As a result, each location within the field of view receives a brief flash of light during each scanner cycle (every 33-40 ms). Here, we used in vivo calcium imaging with AOSLO to investigate the impact of this intermittent stimulation on the retinal ON and OFF pathways. Raster-scanning exaggerated existing ON-OFF pathway asymmetries at high light levels, leading to high baseline activity in ON cells and increased rectification in OFF cells.

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AOSLO光栅扫描视觉刺激对视网膜ON和OFF通路的不对称激活。

自适应光学扫描光眼镜检（AOSLO）实现了高分辨率视网膜成像、眼动追踪和活体眼刺激传递。aoso介导的视觉刺激是通过在视网膜上扫描时暂时调节激发光而产生的。因此，在每个扫描仪周期（每33-40毫秒）内，视场内的每个位置都会接收到短暂的闪光。在这里，我们使用AOSLO的体内钙成像来研究这种间歇性刺激对视网膜on和OFF通路的影响。光栅扫描放大了现有的ON-OFF通路在高光水平下的不对称性，导致ON细胞的高基线活性和OFF细胞的整流增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical optics express BIOCHEMICAL RESEARCH METHODS-OPTICS

CiteScore

6.80

自引率

11.80%

发文量

633

审稿时长

1 months

期刊介绍： The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including: Tissue optics and spectroscopy Novel microscopies Optical coherence tomography Diffuse and fluorescence tomography Photoacoustic and multimodal imaging Molecular imaging and therapies Nanophotonic biosensing Optical biophysics/photobiology Microfluidic optical devices Vision research.