提高远程扫描光场显微镜的空间带宽积。

IF 2.9 2区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Biomedical optics express Pub Date : 2025-02-13 eCollection Date: 2025-03-01 DOI:10.1364/BOE.544498

Aymerick Bazin, Amaury Badon

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引用次数: 0

摘要

实现快速，大规模的体积成像与微米分辨率一直是生物显微镜的一个持久的挑战。为了解决这一挑战，我们报告了一种增强版的光场显微镜，在相机的上游安装了一个电动倾斜镜。根据扫描模式，视野和/或横向分辨率可以大大提高。我们的显微镜技术是简单的，通用的，并配置为亮场和荧光模式。我们用不同形状和大小的多细胞聚集体的成像证明了它的性能。

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Increasing the spatial bandwidth product in light field microscopy with remote scanning.

Achieving fast, large-scale volumetric imaging with micrometer resolution has been a persistent challenge in biological microscopy. To address this challenge, we report an augmented version of light field microscopy, incorporating a motorized tilting mirror upstream of the camera. Depending on the scanning pattern, the field of view and/or the lateral resolution can be greatly improved. Our microscope technique is simple, versatile, and configured for bright-field and epifluorescence modes. We demonstrate its performance with imaging of multi-cellular aggregates of various shapes and sizes.

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来源期刊

Biomedical optics express BIOCHEMICAL RESEARCH METHODS-OPTICS

CiteScore

6.80

自引率

11.80%

发文量

633

审稿时长

1 months

期刊介绍： The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including: Tissue optics and spectroscopy Novel microscopies Optical coherence tomography Diffuse and fluorescence tomography Photoacoustic and multimodal imaging Molecular imaging and therapies Nanophotonic biosensing Optical biophysics/photobiology Microfluidic optical devices Vision research.