Biomedical optics express最新文献

{"title":"Monitoring photobiomodulation of amyloid-β aggregation in 3D cultured cells using label-free nonlinear optical imaging.","authors":"Ting Zhou, Renlong Zhang, Tymish Y Ohulchanskyy, Junle Qu","doi":"10.1364/BOE.549594","DOIUrl":"10.1364/BOE.549594","url":null,"abstract":"<p><p>The accumulation of beta-amyloid (Aβ) peptide aggregates, commonly known as plaques, is considered a key hallmark in the development of Alzheimer's disease (AD). Recently, low-level light therapy (LLLT), also referred to as photobiomodulation (PBM), has emerged as a promising treatment approach for AD. Previous studies have shown that PBM reduces Aβ load primarily by enhancing the clearance capabilities of glia cells. However, it remains unclear whether PBM can directly reduce the formation of Aβ plaques in neuronal cells independent of the glia cell effect. In this study, we employed three-dimensional (3D) cultured HEK 293 APPsw cells as an AD model to investigate the impact of PBM on Aβ aggregation. We demonstrated that label-free two-photon excited fluorescence (TPEF) imaging and second harmonic generation (SHG) imaging are effective tools for monitoring Aβ aggregation in 3D cell models. The TPEF imaging results and subsequent quantification revealed that PBM, particularly with low-level near-infrared light from an 808 nm laser (compared to 1064, 1210, and 1470 nm lasers), significantly reduced Aβ aggregation, specifically plaques formation, in the 3D cultured cells, with the effect found to be dose-dependent. Moreover, a comprehensive analysis of protein expression in the 3D cultured cells revealed that PBM induces overexpression of the LRP1 receptor, which mediates Aβ degradation and thus leads to the reduction of Aβ aggregation. This study highlights the use of label-free nonlinear optical imaging to monitor Aβ aggregation in AD progression and provides novel insights into the effects of PBM on Aβ plaque formation in AD models.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"1143-1155"},"PeriodicalIF":2.9,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomedical Optics Express recognizes the 2024 best paper prize winners: editorial.","authors":"Sina Farsiu, Ruikang K Wang","doi":"10.1364/BOE.559056","DOIUrl":"10.1364/BOE.559056","url":null,"abstract":"<p><p>The Editor-in-Chief and Deputy Editor of <i>Biomedical Optics Express</i> announce the prize for the best paper published in the Journal between 2021 and 2023.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"1156-1157"},"PeriodicalIF":2.9,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introduction to the Biophotonics Congress 2024 feature issue.","authors":"Dongkyun Kang, Anabela Da Silva, Jennifer Lynch, Srivalleesha Mallidi, Michael Pircher, Inga Saknite","doi":"10.1364/BOE.559672","DOIUrl":"10.1364/BOE.559672","url":null,"abstract":"<p><p>We present a feature issue containing 14 papers based on a subset of the studies presented during the Optica Biophotonics Congress: Biomedical Optics held on April 7-10, 2024, in Ft. Lauderdale, Florida, USA.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"1158-1159"},"PeriodicalIF":2.9,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lightweight denoising speckle contrast image GAN for real-time denoising of laser speckle imaging of blood flow.","authors":"Xu Sang, Ruixi Cao, Liushuan Niu, Bin Chen, Dong Li, Qiang Li","doi":"10.1364/BOE.545628","DOIUrl":"10.1364/BOE.545628","url":null,"abstract":"<p><p>To tackle real-time denoising of noisy laser speckle blood flow images, a novel lightweight denoising speckle contrast image generative adversarial network (LDSCI-GAN) is proposed. In the framework, a lightweight denoiser removes noise from the original image, and a discriminator compares the denoised result with the reference one, enabling efficient learning and optimization of the denoising process. With a multi-scale loss function in the log-transformed domain, the training process significantly improves accuracy and denoising by using only five frames of raw speckle images while well-preserving the overall pixel distribution and vascular contours. Animal and phantom experimental results indicate that the LDSCI-GAN can eliminate vascular artifacts while retaining the accuracy of relative blood flow velocity. In terms of peak signal-to-noise ratio (PSNR), mean structural similarity index (MSSIM), and Pearson correlation coefficient (R), the LDSCI-GAN outperforms other deep-learning methods by 3.07 dB, 0.10 (<i>p </i>< 0.001), and 0.09 (<i>p</i> = 0.023), respectively. It has been successfully applied to the real-time monitoring of laser-induced thrombosis. Through conducting tests on the denoising performance of blood flow images of a moving subject, our proposed method achieved enhancements of 23.6% in PSNR, 30% in MSSIM, and 6.5% in the metric R, respectively, when compared to DRSNet. This means that the LDSCI-GAN also shows possible application in handheld devices, offering a potent tool for investigating blood flow and thrombosis dynamics more efficiently and conveniently.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"1118-1142"},"PeriodicalIF":2.9,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hybrid CNN-Mamba model for multi-scale fundus image enhancement.","authors":"Xiaopeng Wang, Di Gong, Yi Chen, Zheng Zong, Meng Li, Kun Fan, Lina Jia, Qiyuan Cao, Qiang Liu, Qiang Yang","doi":"10.1364/BOE.542471","DOIUrl":"10.1364/BOE.542471","url":null,"abstract":"<p><p>This study proposes a multi-scale fundus image enhancement approach that combines CNN with Mamba, demonstrating clear superiority across multiple benchmarks. The model consistently achieves top performance on public datasets, with the lowest FID and KID scores, and the highest PSNR and SSIM values, particularly excelling at larger image resolutions. Notably, its performance improves as the image size increases, with several metrics reaching optimal values at 1024 × 1024 resolution. Scale generalizability further highlights the model's exceptional structural preservation capability. Additionally, its high VSD and IOU scores in segmentation tasks further validate its practical effectiveness, making it a valuable tool for enhancing fundus images and improving diagnostic accuracy.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"1104-1117"},"PeriodicalIF":2.9,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed fiber-bundle fluorescence microscope for quantifying single-cell responses to high-power radiofrequency sources.","authors":"Sean P O'Connor, Aryana J Cruz Santory, Joseph E Clary, Anna V Sedelnikova, Zachary T Brawley, Ryan M Kulow, Gary D Noojin, Kaitlin S Nelson-Rakofsky, Joel N Bixler, Zachary A Steelman","doi":"10.1364/BOE.550033","DOIUrl":"10.1364/BOE.550033","url":null,"abstract":"<p><p>Modern telecommunications systems rely on the ubiquitous use of radiofrequency (RF) fields. To ensure the safety of living systems under RF exposure, standards have been developed which rely on observed thresholds that produce an adverse response. Unfortunately, real-time imaging of single-cell responses to high-peak power RF exposures is experimentally difficult, as high-power RF may damage sensitive electronics such as cameras or photodetectors, and any metal in the exposure zone (such as a microscope objective or translation stage) interacts with the RF by reflecting the RF field, acting as an antenna, or altering the dose delivered to the sample. In this work, we present a custom fluorescence microcopy system compatible with high-power RF environments. Our device uses a custom, 3D-printed objective consisting entirely of plastic and glass components as well as a coherent fiber bundle to relay light between the exposure zone and the fluorescence detection scheme. Our device was validated against a high-end commercial confocal microscope by comparing cellular responses to a well-characterized nanosecond pulsed electric field (nsPEF) stimulus delivered via an electrode pair. Our system performed well under extreme RF exposure, demonstrating continuous fluorescence imaging and maintenance of the focal plane despite >40°C temperature variation at the sample caused by high peak power free-field RF exposure at a frequency of 2.8 GHz. This system is intended to aid researchers in investigating real-time biological responses to radiofrequency and microwave sources.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"1071-1089"},"PeriodicalIF":2.9,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and cost-effective screening of therapeutic targets for isoquercitrin in insulin resistance using virtual methods and fiber SPR biosensing.","authors":"Yong Wei, Yuye Xuan, Wenxiang Wang, Yonghui Zhang, Xiaoshan Li, Chunlan Liu, Chen Wang, Zhihai Liu","doi":"10.1364/BOE.555014","DOIUrl":"10.1364/BOE.555014","url":null,"abstract":"<p><p>The existing screening methods for therapeutic targets of active ingredients in traditional Chinese medicine (TCM) have problems of long detection time and high instrument cost. This article proposes a new target screening method based on virtual screening and fiber surface plasmon resonance (SPR) sensing technology, which has the characteristics of flexibility, speed, and low cost. It also reveals the target mechanism of the active ingredient isoquercitrin in the treatment of insulin resistance (IR). The binding energies of isoquercitrin with target proteins PDPK1, INSR, and PTPN1 were calculated using computer virtual methods to be -8.9, -8.9, -8.8 kcal/mol, indicating strong binding activity with isoquercitrin and predicted as three key targets. Then a fiber optic SPR biosensor functionalized with isoquercitrin molecules was constructed to detect the binding affinity between isoquercitrin and the key targets. The experimental results showed that the binding affinities of isoquercitrin to the targets PDPK1, INSR, and PTPN1 were 1.45, 1.14, and 13.21, respectively, indicating that PTPN1 is the main target of isoquercitrin in the treatment of IR. The proposed sensor has a sensitivity of 0.699 nm/(μg/ml), LOD of 0.515μg/ml, and the experimental detection time of this method is as low as 45 minutes, without the need for large and expensive optical demodulation equipment, and the device volume is 5.50 dm³, providing new ideas for the screening of therapeutic targets of active ingredients in TCM.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"1090-1103"},"PeriodicalIF":2.9,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the general clinical condition and functional properties of the eyes of rabbits after THz irradiation.","authors":"Ekaterina Butikova, Kristina Krasner, Nikolay Kanygin, Elena Drobot, Irina Levchenko, Aleksandr Kichigin, Vasiliy Popik, Daria Kolomeyets, Olga Solovieva, Tatyana Tolstikova, Alina Alshevskaya, Evgeniy Zavyalov, Valeriy Chernykh, Olga Poveshenko, Vladimir Kanygin","doi":"10.1364/BOE.546147","DOIUrl":"10.1364/BOE.546147","url":null,"abstract":"<p><p>THz radiation is increasingly used for diagnostics in medicine. As technology utilizing THz radiation continues to develop rapidly, it is becoming increasingly important to consider its biological effects and establish safe exposure standards and parameters. The paper presents data on the clinical status and functional properties of the anterior and posterior structures of the eyes of rabbits after THz irradiation at the frequency of 2.3 THz. Terahertz radiation was generated at Novosibirsk Free Electron Laser (NovoFEL) at \"Siberian Synchrotron and Terahertz Radiation Centre\" (Budker Institute of Nuclear Physics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia). The exposure durations used were 15 and 30 minutes. Intensity ranges were I1=0.012 mW/cm², I2=0.018 mW/cm², and I3=0.024 mW/cm². The study investigated the effects of various time and power irradiation protocols on the California rabbit's eyes and after a period of one month, but no significant clinical or functional alterations were observed in response to the established intensity protocols. However, the study identified statistically significant changes in corneal hydration and endothelial cell density over time, particularly under protocols with 15- and 30-minute exposures. A negative correlation was found between endothelial cell density and corneal thickness (r=-0.36, p=0.042), suggesting that a reduction in the endothelial cell pool may be associated with increased corneal thickness. These changes were subclinical and did not lead to clinically significant pathological changes in the cornea. There were no signs of ASOCT (anterior segment-optical coherence tomography) hyperreflectivity. THz radiation with parameters listed above of 2.3 THz and an intensities of 0.012-0.024 mW/cm² for 30 minutes has been shown to be conditionally safe for the structures of the rabbit eye. However, the detected subclinical corneal changes require further study to determine safe exposure limits.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"1043-1061"},"PeriodicalIF":2.9,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing the spatial bandwidth product in light field microscopy with remote scanning.","authors":"Aymerick Bazin, Amaury Badon","doi":"10.1364/BOE.544498","DOIUrl":"10.1364/BOE.544498","url":null,"abstract":"<p><p>Achieving fast, large-scale volumetric imaging with micrometer resolution has been a persistent challenge in biological microscopy. To address this challenge, we report an augmented version of light field microscopy, incorporating a motorized tilting mirror upstream of the camera. Depending on the scanning pattern, the field of view and/or the lateral resolution can be greatly improved. Our microscope technique is simple, versatile, and configured for bright-field and epifluorescence modes. We demonstrate its performance with imaging of multi-cellular aggregates of various shapes and sizes.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"1062-1070"},"PeriodicalIF":2.9,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visual simulation of intraocular lenses: technologies and applications [Invited].","authors":"Susana Marcos, Pablo Artal, Linda Lundström, Geunyoung Yoon","doi":"10.1364/BOE.546971","DOIUrl":"10.1364/BOE.546971","url":null,"abstract":"<p><p>Cataract surgery requires selecting an intraocular lens (IOL), whose design affects visual outcomes. Traditional IOL evaluation relies on optical models and bench testing, but these methods fall short in simulating perceptual factors crucial to patient experience. Visual simulators, based on different principles including adaptive optics, temporal multiplexing or physical projection of the IOLs, now allow patients and clinicians to preview and compare different IOL designs preoperatively. By simulating real-world interactions of the eye's optics and the visual system with IOLs, these simulators enhance the patient decision-making process, enable personalized cataract surgery, and can aid in regulatory assessments of IOLs by incorporating pre-operative patient-reported visual outcomes. Visual simulators incorporate deformable mirrors, spatial light modulators and optotunable lenses as dynamic elements to simulate monofocal, multifocal and extended depth-of-focus IOLs, including newer designs aimed at improving contrast sensitivity, expanding depth of focus, and minimizing visual disturbances. With ongoing advancements, these simulators hold potential for transforming IOL design, regulatory processes, and patient care by providing realistic and patient-centered visual assessments, ultimately leading to more successful, individualized surgical outcomes.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 3","pages":"1025-1042"},"PeriodicalIF":2.9,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}