Biomedical optics express最新文献_第2页

Towards real-time diffuse optical tomography with a handheld scanning probe. 走向实时漫射光学断层扫描与手持扫描探头。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-03-26 eCollection Date: 2025-04-01 DOI: 10.1364/BOE.549880

Robin Dale, Nicholas Ross, Scott Howard, Thomas D O'Sullivan, Hamid Dehghani

{"title":"Towards real-time diffuse optical tomography with a handheld scanning probe.","authors":"Robin Dale, Nicholas Ross, Scott Howard, Thomas D O'Sullivan, Hamid Dehghani","doi":"10.1364/BOE.549880","DOIUrl":"https://doi.org/10.1364/BOE.549880","url":null,"abstract":"Diffuse optical tomography (DOT) performed using deep-learning allows high-speed reconstruction of tissue optical properties and could thereby enable image-guided scanning, e.g., to enhance clinical breast imaging. Previously published models are geometry-specific and, therefore, require extensive data generation and training for each use case, restricting the scanning protocol at the point of use. A transformer-based architecture is proposed to overcome these obstacles that encode spatially unstructured DOT measurements, enabling a single trained model to handle arbitrary scanning pathways and measurement density. The model is demonstrated with breast tissue-emulating simulated and phantom data, yielding - for 24 mm-deep absorptions (μ a ) and reduced scattering (μ s ') images, respectively - average RMSEs of 0.0095±0.0023 cm-1 and 1.95±0.78 cm-1, Sørensen-Dice coefficients of 0.55±0.12 and 0.67±0.1, and anomaly contrast of 79±10% and 93.3±4.6% of the ground-truth contrast, with an effective imaging speed of 14 Hz. The average absolute μ a and μ s ' values of homogeneous simulated examples were within 10% of the true values.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 4","pages":"1582-1601"},"PeriodicalIF":2.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12047716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Needle hydrophone-based photoacoustic microscopy with experimentally measured impulse response for improved depth of focus. 针水听器为基础的光声显微镜与实验测量的脉冲响应改善聚焦深度。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-03-26 eCollection Date: 2025-04-01 DOI: 10.1364/BOE.560563

Kisik Kim, Oleksandra Gulenko

{"title":"Needle hydrophone-based photoacoustic microscopy with experimentally measured impulse response for improved depth of focus.","authors":"Kisik Kim, Oleksandra Gulenko","doi":"10.1364/BOE.560563","DOIUrl":"https://doi.org/10.1364/BOE.560563","url":null,"abstract":"Reconstruction-based acoustic-resolution photoacoustic microscopy (AR-PAM) has been developed to extend the depth of field (DOF), enabling simultaneous observation of structures at multiple depths. However, conventional AR-PAM systems, which rely on focused transducers, face inherent limitations in effectively increasing the DOF. To address this issue, we developed a needle hydrophone (NH)-based AR-PAM system that enables deep imaging with enhanced resolution and improved DOF. The proposed system was validated using tissue-mimicking phantoms and ex Ovo chick embryo imaging. Our results demonstrated a DOF exceeding 20 mm, a lateral resolution comparable to the NH diameter (∼400 µm) at shallow depth (10 mm) and 870 µm at deep depth (30 mm), and an axial resolution of 250 µm. Furthermore, we investigated the impact of different reconstruction techniques, including the measured impulse response function (MIRF), simulated impulse response function (SIRF), and coherence factor (CF). Our comparative analysis revealed that MIRF-based reconstruction provided superior performance in maintaining resolution and image quality across varying depths, making it the most effective approach for multi-depth imaging.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 4","pages":"1638-1650"},"PeriodicalIF":2.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12047735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-invasive quantification of total hemoglobin concentrations by visible-light spectroscopic optical coherence tomography: a validation study on healthy volunteers. 通过可见光光谱光学相干断层扫描对总血红蛋白浓度的无创定量：一项健康志愿者的验证研究。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-03-24 eCollection Date: 2025-04-01 DOI: 10.1364/BOE.549671

Carlos Cuartas-Vélez, Isabella Gómez-Gallego, Nienke Bosschaart

{"title":"Non-invasive quantification of total hemoglobin concentrations by visible-light spectroscopic optical coherence tomography: a validation study on healthy volunteers.","authors":"Carlos Cuartas-Vélez, Isabella Gómez-Gallego, Nienke Bosschaart","doi":"10.1364/BOE.549671","DOIUrl":"https://doi.org/10.1364/BOE.549671","url":null,"abstract":"Clinically, a high demand exists for the noninvasive assessment of total hemoglobin concentrations in vulnerable patient groups. Despite the sensitivity of many optical modalities to the absorption of hemoglobin, it has remained challenging to optically measure hemoglobin concentrations noninvasively with sufficient accuracy. Compared to other optical modalities, visible-light spectroscopic optical coherence tomography (vis-sOCT) has the unique ability to quantify optical properties within highly confined tissue volumes. However, total hemoglobin quantification in vivo is still limited due to a lack of dedicated acquisition schemes, processing methods, and validation against a gold standard. In this work, we introduce an approach that combines optical angiography with spatially resolved visible-light spectroscopy to obtain clinically representative estimations of the total hemoglobin concentration in the skin microcirculation. We validate our approach in vivo on a group of 27 healthy volunteers against hemoglobin measurements on venous blood samples with a commercial blood analyzer. The measured hemoglobin concentration by vis-sOCT correlates moderately, but significantly with the blood analyzer (R = 0.47, p = 0.015). This approach quantifies hemoglobin with a mean bias of 0.6 g dL-1 and an average standard deviation of 1.3 g dL-1 in the healthy hemoglobin range (14-19 g dL-1). Moreover, the results highlight the known influence of gender on the comparison between the microcirculatory and venous hemoglobin concentration. This observed influence of gender further suggests that vis-sOCT is sensitive to microcirculatory changes in hemoglobin concentration.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 4","pages":"1569-1581"},"PeriodicalIF":2.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12047714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Black phosphorus enabled non-invasive protein detection with electromagnetic induction well terahertz biosensor chips. 黑磷实现了电磁感应和太赫兹生物传感器芯片的非侵入性蛋白质检测。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-03-24 eCollection Date: 2025-04-01 DOI: 10.1364/BOE.554409

Xin Hu, Wenhao Xu, Xin Chen, Xiaojie Zhao, Xiaoguang Xu, Jing Peng, Qi Song, Bingyuan Zhang, Min Zhang, Hongzhuan Xuan

引用次数: 0

Quantitative assessment of thrombosis-induced blood oxygenation change in deep tissues based on photoacoustic tomography: an ex vivo study. 基于光声断层成像的深部组织血栓形成诱导的血氧变化的定量评估：一项离体研究。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-03-24 eCollection Date: 2025-04-01 DOI: 10.1364/BOE.557086

Yingjie Feng, Qiuqin Mao, Lei Hong, Xiaotian Wang, Chao Tao, Xiaojun Liu

{"title":"Quantitative assessment of thrombosis-induced blood oxygenation change in deep tissues based on photoacoustic tomography: an ex vivo study.","authors":"Yingjie Feng, Qiuqin Mao, Lei Hong, Xiaotian Wang, Chao Tao, Xiaojun Liu","doi":"10.1364/BOE.557086","DOIUrl":"https://doi.org/10.1364/BOE.557086","url":null,"abstract":"The staging and classification of thrombosis hold significant clinical value for optimizing thrombus treatment strategies. In this study, we propose a quantitative method based on photoacoustic tomography for assessing thrombosis in deep tissues. By using inner chromophore signals as a correction factor, this approach minimizes the 'spectral coloring' effects caused by overlying heterogeneous tissues. Ex vivo experiments validate that the method acquires accurate spectra up to a depth of 30 mm across various tissue conditions. After calibration, the Pearson correlation coefficients calculated for the spectrum in deep tissue against the uncolored absorption spectrum is 15% higher, and the standard deviation of the Pearson correlation coefficients decreased by 58%. Sequential measurements capture time-dependent spectral changes of thrombus phantom during six days, providing a potential diagnostic reference for thrombus formation time and type. This method offers a non-invasive, practical tool for accurately quantifying thrombosis stages, which might be valuable for optimizing treatment strategies.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 4","pages":"1557-1568"},"PeriodicalIF":2.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12047737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative assessment of in vivo nuclei and layers of human skin by deep learning-based OCT image segmentation. 基于深度学习的OCT图像分割在体人体皮肤核和层的定量评估。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-03-21 eCollection Date: 2025-04-01 DOI: 10.1364/BOE.558675

Chih-Hao Liu, Li-Wei Fu, Shu-Wen Chang, Yen-Jen Wang, Jen-Yu Wang, Yu-Hung Wu, Homer H Chen, Sheng-Lung Huang

{"title":"Quantitative assessment of in vivo nuclei and layers of human skin by deep learning-based OCT image segmentation.","authors":"Chih-Hao Liu, Li-Wei Fu, Shu-Wen Chang, Yen-Jen Wang, Jen-Yu Wang, Yu-Hung Wu, Homer H Chen, Sheng-Lung Huang","doi":"10.1364/BOE.558675","DOIUrl":"https://doi.org/10.1364/BOE.558675","url":null,"abstract":"Recent advancements in cellular-resolution optical coherence tomography (OCT) have opened up possibilities for high-resolution and non-invasive clinical diagnosis. This study uses deep learning-based models on cross-sectional OCT images for in vivo human skin layers and keratinocyte nuclei segmentation. With U-Net as the basic framework, a 5-class segmentation model is developed. With deeply supervised learning objective functions, the global (skin layers) and local (nuclei) features were separately considered in designing our multi-class segmentation model to achieve an > 85% Dice coefficient accuracy through 5-fold cross-validation, enabling quantitative measurements for the healthy human skin structure. Specifically, we calculate the thickness of the stratum corneum, epidermis, and the cross-sectional area of keratinocyte nuclei as 22.71 ± 17.20 µm, 66.44 ± 11.61 µm, and 17.21 ± 9.33 µm2, respectively. These measurements align with clinical findings on human skin structures and can serve as standardized metrics for clinical assessment using OCT imaging. Moreover, we enhance the segmentation accuracy by addressing the limitations of microscopic system resolution and the variability in human annotations.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 4","pages":"1528-1545"},"PeriodicalIF":2.9,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12047727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-free whole-kidney metabolic optical imaging using in vivo insulated cryofixation and cryo-micro-optical sectioning tomography. 无标记全肾代谢光学成像使用体内绝缘冷冻固定和冷冻显微光学切片断层扫描。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-03-21 eCollection Date: 2025-04-01 DOI: 10.1364/BOE.554000

Guoqing Fan, Mingyu Tian, Huijuan Pan, Chuancao Sun, Haoyu Gong, Qingming Luo, Jing Yuan

引用次数: 0

Chromatic imaging properties of myopia control spectacle lenses. 近视控制眼镜镜片的色差成像特性。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-03-19 eCollection Date: 2025-04-01 DOI: 10.1364/BOE.545932

Augusto Arias, Susanna P Clement, Pablo Artal, Siegfried Wahl

引用次数: 0

Polarimetry through a flexible imaging fiber bundle with a pixelated polarizer. 通过带有像素化偏振器的柔性成像光纤束进行偏振测量。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-03-19 eCollection Date: 2025-04-01 DOI: 10.1364/BOE.554860

Natzem Lima, Clarissa M DeLeon, Travis W Sawyer

引用次数: 0

Identifying phenotypes of colorectal malignant tumors using the quasi-bound state in the continuum of a terahertz metasurface biosensor. 利用太赫兹超表面生物传感器连续体中的准结合状态识别结直肠恶性肿瘤的表型。

IF 2.9 2区医学

Biomedical optics express Pub Date : 2025-03-18 eCollection Date: 2025-04-01 DOI: 10.1364/BOE.557218

Hongji Xu, Hongye Wang, Xiang Yang, Iver Grønlien, Arthur Georg Serville Torvund, Angelos Xomalis, Zhenyu Zhao

{"title":"Identifying phenotypes of colorectal malignant tumors using the quasi-bound state in the continuum of a terahertz metasurface biosensor.","authors":"Hongji Xu, Hongye Wang, Xiang Yang, Iver Grønlien, Arthur Georg Serville Torvund, Angelos Xomalis, Zhenyu Zhao","doi":"10.1364/BOE.557218","DOIUrl":"https://doi.org/10.1364/BOE.557218","url":null,"abstract":"A rapid and non-invasive method to identify phenotypes of colorectal malignant tumors is of vital importance for oncological surgery and further development of corresponding anti-tumor drugs. Herein, we demonstrate an approach to detect colorectal adenocarcinoma and colorectal cancer using the quasi-bound state in the continuum (q-BIC) resonance of a metasurface-based terahertz biosensor. We found that the colorectal adenocarcinoma leads to a 40 GHz q-BIC resonance shift compared to healthy colorectal cells. In addition, we found that colorectal cancer results in a q-BIC resonance red-shift of about 60 to 80 GHz. Both colorectal adenocarcinoma and cancer increase the linewidth of q-BIC resonance compared to healthy colorectal cells. The electric permittivity change confirms the aforementioned frequency shift, which is attributed to the water content of different colorectal malignant tumor cells. Our results highlight that the q-BIC resonance of a terahertz photonic biosensor offers a rapid and non-invasive methodology for identifying different colorectal malignant tumors, which accelerates oncological diagnosis.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 4","pages":"1471-1482"},"PeriodicalIF":2.9,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12047722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0