Biomedical optics express最新文献

{"title":"3D fast ray-traced distortion correction of indirectly viewed intraoperative retinal OCT for surgical tool path reconstruction.","authors":"Yuan Tian, Ali Torkashvand, George T Funkenbusch, Srisatya Pusuluri, Bo Hansen, Florian Kapeller, Michelle McCall, Cynthia A Toth, Anthony N Kuo, Lejla Vajzovic","doi":"10.1364/BOE.606448","DOIUrl":"10.1364/BOE.606448","url":null,"abstract":"<p><p>Intraoperative optical coherence tomography (iOCT) has emerged as a promising modality for real-time three-dimensional image guidance in retinal surgery, enabling visualization of different retinal layers and tool-tissue interactions with micrometer-scale precision. Indirect viewing systems, which employ additional optical elements to increase the surgical field of view (FOV) of the retina, are widely adopted in vitreoretinal procedures. However, the optical design of these indirect viewing systems introduces nonlinear optical distortions that compromise the spatial accuracy required for absolute positioning, such as might be required for robotic end effectors operating in the surgical FOV. In this paper, we present a computationally efficient method to achieve accurate distortion correction between the distorted optical coherence tomography (OCT) image space and the physical surgical workspace through a combination of optical ray tracing and parametric spline fitting. Our approach leverages physics-based ray tracing to characterize the complex optical transformations introduced by the indirect viewing system, then employs numerical spline interpolation to create a fast mapping function suitable for robotic control applications. The spline-based transformation achieves approximately 14 × faster computation than direct commercial software based optical ray tracing. Additionally, we developed a simplified porcine eye model encompassing the cornea through the crystalline lens for use in ray tracing simulations. Validation on <i>ex vivo</i> porcine eyes demonstrated that the proposed method reduced distortion errors along simulated surgical tool insertion paths. Across 30 OCT volumes from three eyes, the reconstructed length of a 7 <i>mm</i> straight tool path deviated from the open-path ground truth by 498 <i>μm</i> before correction and by 19 <i>μm</i> after correction. Over the same volumes, the correction reduced the residual registration error against the ground truth from 150 <i>μm</i> to 90 <i>μm</i>, and the straightness deviation of the reconstructed path from 226 <i>μm</i> to 137 <i>μm</i>, compared with 109 <i>μm</i> for the ground truth itself.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 10","pages":"5193-5212"},"PeriodicalIF":3.2,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13671697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-free, multiphoton microscopy-based strategy for intraoperative grading of lung adenocarcinoma via quantitative extracellular matrix analysis.","authors":"Houqiang Li, Shouzhi Wang, Qiming He, Qianshun Chen, Jianping Huang, Zhan Zhuang, Kangmei Tong, Xunbin Yu, Lan Lin, Xiyao Yan, Jianxin Chen, Shu Wang, Xu Li, Xin Chen","doi":"10.1364/BOE.601927","DOIUrl":"10.1364/BOE.601927","url":null,"abstract":"<p><p>Lung adenocarcinoma (LUAD) requires accurate subtyping for prognosis and treatment, yet conventional histopathology is time-consuming and subjective. This study applies label-free multiphoton microscopy (MPM) to quantify extracellular matrix remodeling, imaging collagen via SHG and elastin via TPEF in 88 specimens from 72 patients. We introduce a SHG/TPEF ratio (STR) system to assess tumor invasiveness. Results show progressive \"fiber desertification,\" reduced fiber content, and decreased collagen alignment in high-grade LUAD subtypes. STR metrics strongly correlate with pathological grading and effectively distinguish aggressive patterns. This MPM-based framework enables rapid, objective intraoperative identification of high-grade LUAD, improving diagnostic precision and clinical decision-making.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 10","pages":"5179-5192"},"PeriodicalIF":3.2,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13671701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149215053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spectrometer matching for balanced detection in spectral-domain optical coherence tomography by autocorrelation minimization.","authors":"Santosh Balakrishnan, Steven G Adie","doi":"10.1364/BOE.610860","DOIUrl":"10.1364/BOE.610860","url":null,"abstract":"<p><p>Precise matching of spectrometers is essential to realizing the full benefits of balanced detection in spectral-domain optical coherence tomography (SD-OCT). A retrospective approach to match spectrometers, based on the observation that the autocorrelation signal should be zero in a perfectly balanced system, is presented. This approach requires only a single calibration B-scan, does not depend on the source noise or image properties, and can be used either to guide alignment or to obtain balanced results from previously acquired data. The performance of this method is demonstrated on a fiber-based SD-OCT system with a pair of commercial spectrometers and for a super-luminescent diode or supercontinuum laser source.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 10","pages":"5164-5178"},"PeriodicalIF":3.2,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13671692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149215032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational optical sensing and machine learning driven optimization of SPR biosensor for colorectal cancer detection.","authors":"Maymona Binte Juwel, M M Atiqur Rahman, Md Tajrian Bhuiyan, Safayat-Al Imam, Khandakar Mohammad Ishtiak","doi":"10.1364/BOE.610145","DOIUrl":"10.1364/BOE.610145","url":null,"abstract":"<p><p>Colorectal cancer biomarkers including methylated SDC2 (mSDC2), methylated SEPT9 (mSEPT9), and carcinoembryonic antigen (CEA), are effective biomarkers for the early detection and monitoring of colorectal cancer. In this study, a high-performance surface plasmon resonance (SPR) biosensor incorporating MgF₂ prism/CaTiO₃/Cu/Al₂O₃/BP multilayers is designed to enhance light-matter interaction and improve sensing performance. The brute force algorithm is employed to optimize the sensor parameters. Also, design is systematically optimized and investigated by using TMM, FEM and FDTD simulations. The accuracy of ANN, ANFIS and RBFNN system models in predicting sensor responses is thoroughly validated. The optimized biosensor achieves a sensitivity of 479.58 deg/RIU, figure of merit of 152.01, and quality factor of 167.86 RIU^-1 at a wavelength of 633 nm, demonstrating the potential of the optimized architecture for sensitive refractive-index sensing relevant to future colorectal cancer biomarker detection.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 10","pages":"5141-5163"},"PeriodicalIF":3.2,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13671690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neckline-free open-source cap design and improved motion resistance for perioperative fNIRS procedures.","authors":"Seth Bolton Crawford, Shimra Jaime Fine, Kyla Inez Covato, Caroline Joyce Caveness, Audrey K Bowden","doi":"10.1364/BOE.605391","DOIUrl":"10.1364/BOE.605391","url":null,"abstract":"<p><p>Functional near-infrared spectroscopy (fNIRS) caps are limited by motion artifacts and unstable optode-scalp coupling, which degrade signal quality during extended and naturalistic monitoring sessions. We present a fabric-based, chinstrap-free headcap designed to improve mechanical stability and optical signal fidelity through enhanced coupling and reduced relative motion. The open-source cap incorporates a two-layer design with slip-resistant materials and a circumferential tightening mechanism. Performance was evaluated over a two-hour protocol in a mock operating room environment, with motion quantified via image-based tracking, signal quality assessed using signal-to-noise ratio (SNR), scalp-coupling index (SCI), and breath-holding responses, and user comfort evaluated with a human factors questionnaire. Results show reduced displacement and robust hemodynamic signals, supporting improved reliability of fNIRS measurements in perioperative conditions.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 10","pages":"5077-5096"},"PeriodicalIF":3.2,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13671698/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149215012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phase-multiplexed continuous Bessel droplet for high-contrast, gapless volumetric two-photon microscopy.","authors":"Ye Fang, Guangbo Zhang, Qianglong Yang, Haiyang Li, Tianfei Zhao, Sisi Zhou, Xuefeng Sun, Chao Zhang, Jiaqing Guo, Puxiang Lai, Rui Hu, Binglin Shen, Junle Qu, Liwei Liu","doi":"10.1364/BOE.612323","DOIUrl":"10.1364/BOE.612323","url":null,"abstract":"<p><p>Extended depth-of-focus (DOF) imaging using Bessel beams has advanced rapid volumetric two-photon fluorescence microscopy (2PFM) by enabling high-resolution imaging across large volumes. However, this technique faces challenges due to significant sidelobe excitation, which reduces image contrast and the signal-to-background ratio (SBR), particularly at high numerical apertures (NAs). Although Bessel droplets have been proposed to suppress sidelobes, they introduce axial discontinuities, leading to incomplete coverage of the sample volume. To achieve comprehensive volumetric imaging of biological specimens, secondary or multiple axial scans with displaced Bessel droplets are typically required, which inevitably increases acquisition time. In this work, we introduce the phase-multiplexed continuous Bessel droplet (PM-CBD), generated via a four-channel phase multiplexing approach, to address these limitations. The PM-CBD suppresses sidelobes effectively while maintaining the extended DOF and diffraction-free properties of Bessel beams. By eliminating the axial gaps present in Bessel droplet imaging, PM-CBD enables rapid, high-contrast, and complete volumetric imaging. Our results show that PM-CBD improves the SBR, and reduces primary sidelobes by approximately 40% compared to traditional Bessel beams. This technique provides a practical enhancement to existing 2PFM platforms, offering improved imaging performance for <i>in vivo</i> applications in neuroscience and developmental biology.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 10","pages":"5097-5112"},"PeriodicalIF":3.2,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13671687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149215005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optically bandlimited coherent lateral resampling for optical coherence tomography.","authors":"Changhoe Lee, Taeguk Um, Sunhong Jeong, Dongjae Yang, Wang-Yuhl Oh, Byungkun Lee","doi":"10.1364/BOE.608953","DOIUrl":"10.1364/BOE.608953","url":null,"abstract":"<p><p>We present an optically bandlimited coherent lateral resampling framework for densely and nonuniformly sampled optical coherence tomography (OCT). Fourier-domain group-delay stabilization is combined with bandlimited DFT/NUDFT reconstruction and evaluated using retinal OCT acquired at a 4.73 MHz A-scan rate. When the target-grid Nyquist remains at or above the retained optical cutoff, reconstruction density can be selected retrospectively without changing the retained spectral support. Experiments demonstrate alias suppression, correction of nonuniform scan distortions, and improved SNR associated with dense acquisition relative to the retained optical bandwidth. The framework enables densely sampled high-speed OCT data to be acquired once and subsequently reconstructed at application-specific lateral sampling densities while explicitly controlling the retained spatial-frequency bandwidth and preserving the SNR benefits of oversampled acquisition.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 10","pages":"5113-5140"},"PeriodicalIF":3.2,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13671694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149215043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperspectral imaging photoplethysmography: insights into the signals' origin and usage.","authors":"Tobias Vogelsang, Vincent Fleischhauer, Alexander Woyczyk, Michael Wellnhofer, Stefan Rasche, Sebastian Zaunseder","doi":"10.1364/BOE.605828","DOIUrl":"10.1364/BOE.605828","url":null,"abstract":"<p><p>This work explores the hyperspectral characteristics of imaging photoplethysmography (iPPG) to (1) deepen the understanding of iPPG signals' origin, and (2) assess iPPG's capacity to capture the peripheral perfusion. Our analyses are based on an experimental study employing a hyperspectral snapshot camera for iPPG and laser speckle contrast imaging (LSCI) at the forearm. We demonstrate a pronounced correlation between the wavelength-dependent normalized AC component from iPPG and the extinction coefficient of oxyhemoglobin (<i>r</i> = 0.975). Further, we show iPPG's ability to explain LSCI (linear mixed models' <i>β</i> up to 0.692). Considering the entire optical spectrum, our results support blood volume changes as a dominant factor for iPPG signals' generation and demonstrate iPPG's capacity to monitor peripheral perfusion.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 10","pages":"5062-5076"},"PeriodicalIF":3.2,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13671670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of end-coated fibre-optic ultrasound transmitters.","authors":"Semyon Bodian, Shaoyan Zhang, Nyma Nassren, Edward Z Zhang, Paul C Beard, Richard J Colchester, Adrien E Desjardins, Erwin J Alles","doi":"10.1364/BOE.608606","DOIUrl":"10.1364/BOE.608606","url":null,"abstract":"<p><p>Obliquely emitting fibre-optic optical ultrasound (OpUS) probes show strong potential for guiding minimally invasive procedures, but the imprecise coating methods used to date deposit excess coating along the side of the fibre, which has been suspected to cause imaging artefacts. In this study, we aimed to confirm the source of these artefacts and to introduce an alternative coating method. Optical fibres cleaved at 40° were coated either by conventional dip-coating (DC) or by a new dip-transfer (DT) method, where the curvature of coating droplets allows for accurate alignment of angle-cleaved end-faces. To demonstrate the advantage of DT over DC deposition, fibre-optic ultrasound sources were fabricated using both methods and thoroughly characterised using planar and cylindrical acoustic field scans. Furthermore, a source fabricated with each method was assembled into a fibre-optic OpUS imaging probe and used to image a controlled phantom. DT transmitters generated significantly higher ultrasound pressures than DC transmitters (1.64 ± 0.14 MPa vs 0.53 ± 0.22 MPa, p < 0.05), with comparable mean bandwidths (24.7 ± 1.6 MHz vs 17.3 ± 6.9 MHz) with significantly lower variability. Cylindrical field scans revealed that DC transmitters exhibited strong secondary acoustic lobes (20% of primary beam amplitude), whereas sources fabricated with the DT method did not generate detectable spurious emissions. Imaging trials confirmed that coating of the fibre sides is the primary source of artefacts in obliquely emitting OpUS probes. The DT method effectively eliminates these artefacts while simultaneously enhancing acoustic performance. The higher acoustic performance and the absence of spurious emissions and their associated image artefacts will further advance the quality of OpUS image guidance of minimally invasive interventions. Furthermore, this study reinforces the need for specialised coating methods for obliquely-viewing OpUS probes and highlights the importance the coating coverage in determining image quality.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 10","pages":"5048-5061"},"PeriodicalIF":3.2,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13671700/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diffusion model with central annotation propagation for segmentation of ellipsoid zone maps on OCT scans of retinitis pigmentosa.","authors":"Kevin C Choy, Glenn J Jaffe, Sina Farsiu","doi":"10.1364/BOE.601785","DOIUrl":"10.1364/BOE.601785","url":null,"abstract":"<p><p>We present a fully automatic algorithm to segment the ellipsoid zone (EZ) on optical coherence tomography (OCT) images of retinitis pigmentosa (RP). We introduce Diffusion Projection U-Net (DPUN), a method to generate en face EZ maps from 3D OCT scans by leveraging diffusion models' state-of-the-art generative capabilities. We further propose Central Annotation Propagation (CAP), a semi-automatic approach that incorporates the segmentation of the central scan to segment all scans in a volume using a memory-reading paradigm. We demonstrated the effectiveness of our methods on a dataset consisting of 60 eyes from 60 participants enrolled in an international, multicenter, phase 3 RP clinical trial (NCT05537220) imaged with spectral-domain OCT at the baseline visit. DPUN outperformed all comparison methods for fully automatic segmentation. DPUN-CAP, which utilized central annotations, achieved the best performance of all models evaluated. The proposed methods produced measurements that were consistent with manual measurements of the EZ area, highlighting their potential utility for clinical trials in which EZ area is a key anatomic endpoint and for monitoring EZ loss progression and treatment response in clinical practice.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"17 10","pages":"5015-5033"},"PeriodicalIF":3.2,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13671693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}