Biology of Reproduction最新文献_第6页

SRA deficiency induces follicular dysplasia by disrupting the hypothalamic Kisspeptin-GPR54 system in mice.

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-03-09 DOI: 10.1093/biolre/ioaf049

Jing Jin, Xinhui Kou, Xinzhe Wang, Xue Yun, Yinyin Ding, Keshu Cai, Yongning Zhai, Huifang Zhou

{"title":"SRA deficiency induces follicular dysplasia by disrupting the hypothalamic Kisspeptin-GPR54 system in mice.","authors":"Jing Jin, Xinhui Kou, Xinzhe Wang, Xue Yun, Yinyin Ding, Keshu Cai, Yongning Zhai, Huifang Zhou","doi":"10.1093/biolre/ioaf049","DOIUrl":"https://doi.org/10.1093/biolre/ioaf049","url":null,"abstract":"Purpose: To investigate how steroid receptor RNA activator (SRA) regulates follicular development in mice.Methods: Systemic SRA knockout mice were introduced. SRA expression was reinstated in the anteroventral periventricular nucleus (AVPV) of the hypothalamus using lentiviral vectors. Subsequently, the estrous cycle, serum hormone levels, follicle development, and hypothalamic kisspeptin expression in mice were assessed. Kiss1 promoter activity was tested with a fluorescent reporter system in Neuro-2a cells.Results: SRA deficiency caused a shift to shorter metestrus and longer diestrus phases, reduced numbers of large antral and preovulatory follicles, increased formation of atretic cyst-like follicles, lowered serum levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and estradiol (E2), and decreased expression of hypothalamic AVPV-kisspeptin in mice. The reinstatement of SRA expression in the AVPV nucleus normalized kisspeptin expression, hormone levels, and follicle development. In Neuro-2a cells, SRA increased Kiss1 transcription upon E2 treatment, a response that was nullified by the estrogen receptor alpha (ERα) inhibitor.Conclusion: SRA enhances ERα-mediated Kiss1 transcription in the AVPV nucleus to control the kisspeptin-GPR54 system in hypothalamus, essential for regulating ovulation through the hypothalamus-pituitary-ovary axis.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal probiotic supplementation and effects on the Fetal placenta.

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-03-09 DOI: 10.1093/biolre/ioaf041

Rosalind T B Herrington, David T Ellenberger, Cheryl S Rosenfeld

{"title":"Maternal probiotic supplementation and effects on the Fetal placenta.","authors":"Rosalind T B Herrington, David T Ellenberger, Cheryl S Rosenfeld","doi":"10.1093/biolre/ioaf041","DOIUrl":"https://doi.org/10.1093/biolre/ioaf041","url":null,"abstract":"Increasing number of pregnant women are consuming probiotics to promote their own health and that of their unborn fetus. Such supplements are presumed to be safe for pregnant mothers and their unborn offspring. For pregnant mothers, such bioactive compounds might lower the risk of constipation, diarrhea, other gastrointestinal conditions, pre-term birth, and prevent adverse pregnancy outcomes, including gestational diabetes mellitus (GDM) and depression/anxiety. More research is needed to examine potential safety of probiotic consumption during pregnancy and long-term health consequences to offspring. The conceptus can also be indirectly affected by maternal probiotic supplementation through microorganism production of bioactive compounds. The placenta is in direct communication with the underlying uterine tissue. Thus, compounds in the maternal blood can easily transfer across the placenta and impact this hormonally sensitive organ. Select studies suggest that disruptions to the maternal microbiome dramatically affects the placenta. In the current review, we will therefore consider the evidence to date of how maternal probiotic supplementation affects the placenta. Three potential mechanisms we will explore include the possibility that maternal probiotic supplementation might impact the putative placenta microbiome. The second potential mechanism we will consider is that maternal probiotic consumption alters bacterial-derived metabolites, including short-chained fatty acids, polyamines, Vitamin B9, and Vitamin B12. The third potential mechanism to be discussed is that such supplements affect maternal and placental immune responses. Before probiotics are promoted for healthy pregnant women and those with gestational disorders, more studies, including those examining the effects on the placenta, are essential.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Equine in vitro fertilization with frozen-thawed semen is associated with shortened pre-incubation time and modified capacitation-related changes.

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-03-09 DOI: 10.1093/biolre/ioaf043

Matheus R Felix, Tamara Dobbie, Elizabeth Woodward, Renata Linardi, Carolina Okada, Rebeca Santos, Katrin Hinrichs

{"title":"Equine in vitro fertilization with frozen-thawed semen is associated with shortened pre-incubation time and modified capacitation-related changes.","authors":"Matheus R Felix, Tamara Dobbie, Elizabeth Woodward, Renata Linardi, Carolina Okada, Rebeca Santos, Katrin Hinrichs","doi":"10.1093/biolre/ioaf043","DOIUrl":"https://doi.org/10.1093/biolre/ioaf043","url":null,"abstract":"We recently reported successful equine IVF using fresh semen pre-incubated for a prolonged period (22 h) before co-culture with oocytes. In this study, we evaluated the feasibility of equine IVF with frozen-thawed sperm and evaluated capacitation-related changes in these sperm over the pre-incubation period. Sperm selected via a commercial sperm separation device (SSD) yielded significantly higher fertilization than did sperm selected by swim-up or by colloid centrifugation. Using the SSD method, fertilization rates with sperm pre-incubated for 15 min, 3 h, 6 h, and 9 h were 7.1, 22.2, 38.5, and 73.3% respectively (9 h vs. 15 min or 3 h, P < 0.05). Fertilization rates differed significantly (45.9% vs. 85.5%) between freezing extenders. Blastocysts were produced using frozen-thawed semen from each of three stallions and transfer of 9 vitrified-warmed blastocysts to mares yielded 7 embryonic vesicles. Anti-protein tyrosine phosphorylation staining of the entire sperm tail increased over pre-incubation, and sperm both with and without staining in the tail bound to the oocyte cumulus after co-incubation. Using the stain DiSC3(5) and flow cytometric analysis, a population of apparently hyperpolarized sperm was identified at 22 h in fresh sperm that was not seen at any time in frozen-thawed sperm. We conclude that frozen-thawed equine sperm can successfully fertilize oocytes after a shortened pre-incubation time of 9 h, suggesting that the freeze-thawing process induces capacitation-related changes. Our findings on evaluation of pre-incubated sperm indicate that the mechanisms by which frozen-thawed sperm become capable of fertilization may differ from those found in fresh sperm.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-genome transcriptome and DNA methylome analyses reveal molecular abnormalities during the oocyte-to-embryo transition in preimplantation embryos derived from prepubertal lamb oocytes.

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-03-09 DOI: 10.1093/biolre/ioaf045

Qi Qi, Jiangyue Bian, Junjin Li, Kexiong Liu, Fengxiang Yan, Jian Hou

{"title":"Whole-genome transcriptome and DNA methylome analyses reveal molecular abnormalities during the oocyte-to-embryo transition in preimplantation embryos derived from prepubertal lamb oocytes.","authors":"Qi Qi, Jiangyue Bian, Junjin Li, Kexiong Liu, Fengxiang Yan, Jian Hou","doi":"10.1093/biolre/ioaf045","DOIUrl":"https://doi.org/10.1093/biolre/ioaf045","url":null,"abstract":"The juvenile in vitro embryo transfer (JIVET) technology holds the potential to accelerate livestock breeding. However, its application is limited due to the weak in vitro development of oocytes and embryos from prepubertal lambs. To dissect the regulatory networks of gene expression of sheep in vitro embryos and identify the defects in gene expression in prepubertal lamb embryos during the oocyte-to-embryo transition (OET), full-length RNA sequencing (RNA-seq) and whole-genome bisulfite sequencing (WGBS) based on trace cells were conducted on in vitro-derived embryos generated from adult sheep and prepubertal lamb oocytes. We found that the maternal mRNA degradation occurred selectively in adult sheep embryos in multiple waves and was most completed until the morula stage. Major embryonic genome activation (EGA) was found to occur at the morula stage. By comparing with the patterns of adult embryos, we observed incomplete maternal mRNA degradation and abnormal EGA in lamb embryos and analyzed their potential molecular mechanisms. Furthermore, we explored dynamic DNA methylation concerning the paternal and maternal genomes during the preimplantation development of sheep embryos, revealing the negative regulatory role of promoter DNA methylation on EGA process. Lamb embryos generally displayed higher DNA methylation levels than adults, potentially repressing the EGA gene expression, especially the genes associated with ribosomal and mitochondrial organization. We also found abnormalities in the methylation status of imprinted genes in lamb embryos. Our findings advance the understanding of sheep in vitro embryo development and offer insights for improving the JIVET technology in livestock.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of progesterone receptor in smooth muscle cells has no impact on oviductal contraction and preimplantation embryo development.

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-03-09 DOI: 10.1093/biolre/ioaf042

Emily A McGlade, Jiude Mao, Kalli K Stephens, Cayce N Rose, John P Lydon, Wipawee Winuthayanon

引用次数: 0

Phosphatase and tensin homolog deficiency induces M2 macrophage polarization by promoting glycolytic activity in endometrial stromal cells.

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-03-04 DOI: 10.1093/biolre/ioaf016

Fengqin Dai, Jinjin Li, Yingwei Liu

{"title":"Phosphatase and tensin homolog deficiency induces M2 macrophage polarization by promoting glycolytic activity in endometrial stromal cells.","authors":"Fengqin Dai, Jinjin Li, Yingwei Liu","doi":"10.1093/biolre/ioaf016","DOIUrl":"https://doi.org/10.1093/biolre/ioaf016","url":null,"abstract":"Endometriosis is a common gynecological disorder, whose pathogenesis remains incompletely understood. Macrophages, a key type of immune cell, are pivotal in the context of endometriosis. This study seeks to explore the interactions between endometriotic cells and macrophages. Quantitative real-time PCR (qRT-PCR) and Western blot experiments were employed to detect phosphatase and tensin homolog (PTEN) expression. Glucose consumption, lactate production, extracellular acidification rate, and oxygen consumption rate levels were used to assess cellular glycolytic capacity. The interaction between conditioned media from ectopic endometrial stromal cells (EESCs) and macrophages was investigated through co-culture experiments. The expression of M2 macrophage marker proteins and inflammatory factors was detected via qRT-PCR, immunofluorescence staining, and enzyme-linked immunosorbent assay. Cellular functions were evaluated using Cell Counting Kit-8, 5-Ethynyl-2'-deoxyuridine (EdU), and wound healing assays. We found that PTEN deficiency promoted the glycolytic activity of EESCs. Simultaneously, it significantly promoted the macrophages' polarization toward the M2 phenotype, demonstrated by increased expression of M2 markers (differentiation 206 (CD206), CD163, and (C-C motif) ligand 22 (CCL22)). Further studies revealed that PTEN-deficient EESCs increased the level of CCL2 via promoting glycolytic activity, which was reversed by glycolytic inhibitor. Moreover, lactate and conditioned media from overexpressed CCL2 EESCs facilitated M2 polarization of macrophages, while 2-deoxy-d-glucose reversed the promoting effect. Furthermore, lactate-facilitated macrophages promoted the proliferation and migration abilities of EESCs. PTEN deficiency induces M2 macrophage polarization by promoting glycolytic activity in EESCs, which deepens the knowledge of the pathophysiology of endometriosis and provides novel insights into its treatment.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Digital analysis of ovarian tissue: generating a standardized method of follicle analysis.

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-03-03 DOI: 10.1093/biolre/ioaf039

引用次数: 0

Single cell multiome analysis of the bovine placenta identifies gene regulatory networks in trophoblast differentiation†.

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-02-23 DOI: 10.1093/biolre/ioaf036

Kimberly M Davenport, Makenzie T Lowke, M Sofia Ortega, Andrew M Kelleher, Wesley C Warren, Thomas E Spencer

{"title":"Single cell multiome analysis of the bovine placenta identifies gene regulatory networks in trophoblast differentiation†.","authors":"Kimberly M Davenport, Makenzie T Lowke, M Sofia Ortega, Andrew M Kelleher, Wesley C Warren, Thomas E Spencer","doi":"10.1093/biolre/ioaf036","DOIUrl":"https://doi.org/10.1093/biolre/ioaf036","url":null,"abstract":"A central determinant of successful reproduction is pregnancy establishment and maintenance that relies on proper development of the conceptus (embryo/fetus and associated extraembryonic membranes including the placenta). Pregnancy loss in cattle can be caused by inadequate development and differentiation of the placenta. However, the cellular and molecular mechanisms regulating bovine placenta development and, particularly, trophoblast differentiation are not well understood. Recent single-cell RNA-seq analyses revealed dynamic changes in cell populations and gene expression patterns during bovine placental development. Here, the chromatin accessibility landscape across diverse cell populations was determined in the developing (day 40) and mature (day 170) bovine placenta using the 10X Genomics multiome (snRNA-seq and snATAC-seq) platform. Analyses revealed distinct trophoblast, mesenchyme, endothelial, immune, and epithelial cell populations characterized by unique gene expression and chromatin accessibility signatures. ATAC-seq peaks defined open chromatin regions, facilitating the identification of transcription factor binding sites and candidate gene regulatory networks (GRN) involved with trophoblast differentiation. Several transcription factors, known for their involvement in trophoblast differentiation in other mammalian species, were identified as candidate regulators of uninucleate (UNC) to binucleate (BNC) trophoblast differentiation. This study adds to our foundational understanding of gene regulation and expression in the placenta, offering insights into the mechanisms governing pregnancy loss in cattle.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppression of FOXO3 by BMP signaling contribute to the different primordial germ cell proliferation between layers and broilers.

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-02-23 DOI: 10.1093/biolre/ioaf037

Yuxiao Ma, Lu Meng, Jiahui Wei, Wenhui Wu, Yun Zhang, Xuzhao Wang, Xiaotong Guo, Feiyi Wang, Yong Mao, Guiyu Zhu

{"title":"Suppression of FOXO3 by BMP signaling contribute to the different primordial germ cell proliferation between layers and broilers.","authors":"Yuxiao Ma, Lu Meng, Jiahui Wei, Wenhui Wu, Yun Zhang, Xuzhao Wang, Xiaotong Guo, Feiyi Wang, Yong Mao, Guiyu Zhu","doi":"10.1093/biolre/ioaf037","DOIUrl":"https://doi.org/10.1093/biolre/ioaf037","url":null,"abstract":"Although all domestic chicken breeds originate from their wild relatives, the red junglefowl, they have been selectively bred for high yields in egg or meat production， or both. Some breeds are highly efficient in egg production, while others perform poorly, due to long-term selection aimed for different purposes. Female primordial germ cells (PGCs) are the precursors of eggs and the population size of PGCs will ultimately determine ovarian reserve of hens. In this study, we observed that the layers exhibit greater proliferation capacity and a higher number of PGCs compared to the broilers before meiosis. By comparing the PGC transcriptomes between layers and broilers, we identified potential genes that regulate cell proliferation. We further confirmed that FOXO3 expression is higher in broilers, where it inhibits the PGC proliferation both in vivo and in vitro. However, in layers, the upstream BMP signaling stimulate the phosphorylation of AKT and suppress FOXO3 activity. Consequently, the elevated BMP signaling and reduced FOXO3 co-operatively promote more robust PGC proliferation in layers compared to broilers and result in a greater number of PGCs in layers. Our data not only reveal molecular mechanisms underlying PGC growth, but also provide new clues to improve the laying performance in chicken.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in uterine and serum metabolome associated with clinical cure failure of metritis in dairy cows.

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-02-23 DOI: 10.1093/biolre/ioaf038

F N S Pereira, A Martelo Pereira, K N Galvão, R S Bisinotto, C C Figueiredo

{"title":"Differences in uterine and serum metabolome associated with clinical cure failure of metritis in dairy cows.","authors":"F N S Pereira, A Martelo Pereira, K N Galvão, R S Bisinotto, C C Figueiredo","doi":"10.1093/biolre/ioaf038","DOIUrl":"https://doi.org/10.1093/biolre/ioaf038","url":null,"abstract":"This study investigated differences in uterine and serum metabolome associated with clinical cure failure of metritis in dairy cows. Metritis was diagnosed in lactating Holstein cows from two Florida dairies and defined by the presence of fetid, watery, reddish-brown vaginal discharge from 4 to 12 days postpartum (dpp). Cows with metritis (n = 24) were paired with cows without metritis of similar parity and dpp (n = 24). On the day of metritis diagnosis (day 0), all cows with metritis received antimicrobial therapy. The continued presence of the fetid, watery, reddish-brown discharge on day 5 (n = 16) was defined as clinical cure failure, whereas clinical cure was defined by its absence (n = 8). Metabolome analyses of uterine lavage (days 0 and 5) and serum samples (day 0) were conducted using untargeted gas chromatography time-of-flight mass spectrometry. Normalized data were analyzed using partial least squares-discriminant analysis and ANOVA, adjusting P-values for multiple comparisons. Differences in the uterine metabolome on day 0 associated with clinical cure failure were linked to carbohydrate, amino acid, and lipid metabolism. Greater concentrations of arachidonic acid, ribose, and glutaric acid were associated with clinical cure failure, suggesting a greater degree of tissue lesion and inflammation. No differences in the serum metabolome were associated with cure failure. No differences in uterine metabolome were associated with clinical cure failure on day 5. The findings suggest that clinical cure failure is associated with a greater uterine inflammatory process that did not persist until cure assessment day.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0