Biology of Reproduction最新文献_第6页

Induction of in vivo-like ciliation in confluent monolayers of re-differentiated equine oviduct epithelial cells†. 在再分化的马输卵管上皮细胞汇聚单层中诱导活体样凋亡。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-14 DOI: 10.1093/biolre/ioae090

Bart Leemans, Bart M Gadella, Josephine H E A M Marchand, Ann Van Soom, Tom A E Stout

{"title":"Induction of in vivo-like ciliation in confluent monolayers of re-differentiated equine oviduct epithelial cells†.","authors":"Bart Leemans, Bart M Gadella, Josephine H E A M Marchand, Ann Van Soom, Tom A E Stout","doi":"10.1093/biolre/ioae090","DOIUrl":"10.1093/biolre/ioae090","url":null,"abstract":"We recently developed re-differentiated equine oviduct epithelial cell (REOEC) monolayers demonstrating various in vivo morphological characteristics, but lacking secondary ciliation. In this study, we evaluated the effects of fetal bovine serum, reproductive steroid hormones, Wnt- and Notch ligands and inhibitors, and different EOEC seeding densities, in both conventional wells and on microporous membranes, on EOEC morphology and, in particular, secondary ciliation. REOEC monolayers were assessed by confocal microscopy after combined staining of nuclei, cilia, and the cytoskeleton. Only Wnt ligands, Notch inhibitors and oviduct explant cell concentration affected EOEC morphology. Undesirable epithelial-mesenchymal transition was observed in REOEC monolayers exposed to Wnt3a containing medium and Wnt ligand CHIR 99021. With respect to secondary ciliation, only the combined effect of oviduct explant cell concentration and Notch inhibition steered REOEC monolayers to in vivo-like ciliation patterns. De-differentiated EOECs, formed 10 days after oviduct explant cell seeding, were reseeded on inserts; only at initial oviduct explant cell concentrations of 1 and 5 × 106 cells per well was the formation of REOEC monolayers with a high rate of diffuse ciliation supported. Within 1 month after air-liquid interface introduction, >40% and >20% of the REOECs showed secondary cilia, respectively. At higher oviduct explant cell seeding densities secondary ciliation was not supported after re-differentiation. Additionally, Notch inhibition helped boost secondary ciliation rates to >60% in REOEC monolayers with diffuse ciliation only. These monolayers demonstrated higher clathrin expression under follicular phase conditions. Overall, the ciliated REOEC monolayers better resemble in vivo oviduct epithelial cells than previous models.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11402525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141282951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Activation of HAND2-FGFR signaling pathway by lncRNA HAND2-AS1 in adenomyosis. 更正：lncRNA HAND2-AS1 在子宫腺肌病中激活 HAND2-FGFR 信号通路。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-14 DOI: 10.1093/biolre/ioae091

引用次数: 0

NR4A1-mediated regulation of lipid droplets in progesterone synthesis in goat luteal cells†. NR4A1 介导的山羊黄体细胞黄体酮合成中脂滴的调控†。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-14 DOI: 10.1093/biolre/ioae085

Hao Yu, Xiaotong Li, Jie Zhao, Wei Wang, Quanwei Wei, Dagan Mao

{"title":"NR4A1-mediated regulation of lipid droplets in progesterone synthesis in goat luteal cells†.","authors":"Hao Yu, Xiaotong Li, Jie Zhao, Wei Wang, Quanwei Wei, Dagan Mao","doi":"10.1093/biolre/ioae085","DOIUrl":"10.1093/biolre/ioae085","url":null,"abstract":"Nuclear receptor NR4A1 is a key factor in glycolipid metabolism and steroidogenesis, while lipid droplets serve as crucial dynamic organelles for lipid metabolism in luteal cells. To investigate the effects of NR4A1 on lipid droplet metabolism and progesterone (P4) synthesis in goat corpus luteum in vitro, luteal cells from the middle-cyclic corpus luteum were isolated and treated with Cytosporone B (CSNB, an agonist) or siRNA of NR4A1. Results showed that both low (1 μM) and high (50 μM) concentrations of CSNB promoted lipid droplet accumulation, while NR4A1 knockdown reduced lipid droplet content. CSNB increased while siNR4A1 decreased total cholesterol content; however, CSNB and siNR4A1 did not change triglyceride content. CSNB increased the expression of perilipins at mRNA and protein levels, also increased LDLR, SCARB1, SREBFs, and HMGCR mRNA abundance. Treatment with siNR4A1 revealed opposite results of CSNB, except for HMCGR and SREBF2. For steroidogenesis, 1 μM CSNB increased, but 50 μM CSNB inhibited P4 synthesis, NR4A1 knockdown also reduced the P4 level. Further analysis demonstrated that 1 μM CSNB increased the protein levels of StAR, HSD3B, and P-HSL, while 50 μM CSNB decreased StAR, HSD3B, and CYP11A1 protein levels. Moreover, 50 μM CSNB impaired active mitochondria, reduced the BCL2, and increased DRP1, Caspase 3, and cleaved-Caspase 3 protein levels. siNR4A1 consistently downregulated the P-HSL/HSL ratio and the steroidogenic protein levels. In conclusion, NR4A1-mediated lipid droplets are involved in the regulation of progesterone synthesis in goat luteal cells.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obese mice have decreased uterine contractility and altered energy metabolism in the uterus at term gestation†. 肥胖小鼠在足月妊娠时子宫收缩力下降，子宫内的能量代谢发生改变。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-14 DOI: 10.1093/biolre/ioae086

Kevin K Prifti, Ronald McCarthy, Xiaofeng Ma, Brian N Finck, Sarah K England, Antonina I Frolova

{"title":"Obese mice have decreased uterine contractility and altered energy metabolism in the uterus at term gestation†.","authors":"Kevin K Prifti, Ronald McCarthy, Xiaofeng Ma, Brian N Finck, Sarah K England, Antonina I Frolova","doi":"10.1093/biolre/ioae086","DOIUrl":"10.1093/biolre/ioae086","url":null,"abstract":"Over 35% of reproductive-age women in the USA have obesity, putting them at increased risk for numerous obstetric complications due to abnormal labor. While the association between maternal obesity and abnormal labor has been well documented, the mechanisms responsible for this remain understudied. The uterine smooth muscle, myometrium, has high energy needs in order to fuel regular uterine contractions during parturition. However, the precise mechanisms by which the myometrium meets its energy demands has not been defined. Here, our objective was to define the effects of obesity on energy utilization in the myometrium during labor. We generated a mouse model of maternal diet-induced obesity and found that these mice had a higher rate of dystocia than control chow-fed mice. Moreover, compared to control chow-fed mice, DIO mice at term, both before and during labor had lower in vivo spontaneous uterine contractility. Untargeted transcriptomic and metabolomic analyses suggest that diet-induced obesity is associated with elevated long-chain fatty acid uptake and utilization in the uterus, but also an accumulation of medium-chain fatty acids. Diet-induced obesity uteri also had an increase in the abundance of long chain-specific beta-oxidation enzymes, which may be responsible for the observed increase in long-chain fatty acid utilization. This altered energy substrate utilization may be a contributor to the observed contractile dysfunction.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11402524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141299955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Klotho accelerates the progression of polycystic ovary syndrome through promoting granulosa cell apoptosis and inflammation†. Klotho 通过促进颗粒细胞凋亡和炎症，加速了多囊卵巢综合症的进展。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-14 DOI: 10.1093/biolre/ioae094

Wenting Ye, Siyu Xia, Tingting Xie, Huiyun Ye, Yi Yang, Yan Sun, Jing Cai, Xiaoqing Luo, Lili Zhou, Yali Song

{"title":"Klotho accelerates the progression of polycystic ovary syndrome through promoting granulosa cell apoptosis and inflammation†.","authors":"Wenting Ye, Siyu Xia, Tingting Xie, Huiyun Ye, Yi Yang, Yan Sun, Jing Cai, Xiaoqing Luo, Lili Zhou, Yali Song","doi":"10.1093/biolre/ioae094","DOIUrl":"10.1093/biolre/ioae094","url":null,"abstract":"The morbidity of polycystic ovary syndrome (PCOS) is in highly increasing rate nowadays. PCOS not only affects the fertility in women, but also threatens the health of whole life. Hence, to find the prognostic risk factors is of great value. However, the effective predictors in clinical practice of PCOS are still in blackness. In this study, we found Klotho (KL) was increased in follicular fluid (FF) and primary luteinized granulosa cells (GCs) from PCOS patients with hyperandrogenism. Furthermore, we found follicular KL was negatively correlated with numbers of mature oocytes, and positively correlated with serum testosterone, LH, and LH/FSH levels menstrual cycle and number of total antral follicles in PCOS patients. In primary luteinized GCs, the increased KL was accompanied with upregulation of cell apoptosis and inflammation-related genes. In ovaries of PCOS mice and cultured human KGN cell line, KL was up-regulated and accompanied by apoptosis, inflammation, and mitochondrial dysfunction. Therefore, our findings suggest new mechanisms for granulosa cell injury and revealed to target inhibit KL maybe a new therapeutic strategy for treatment of PCOS.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential effect of melatonin on in vitro oocyte maturation and embryo development in animals. 褪黑激素对动物体外卵母细胞成熟和胚胎发育的潜在影响。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-14 DOI: 10.1093/biolre/ioae077

Parisa Nadri, Azadeh Zahmatkesh, Azizollah Bakhtari

引用次数: 0

Klinefelter syndrome: etiology and clinical considerations in male infertility†. 克氏综合征：男性不育症的病因和临床考虑因素。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-14 DOI: 10.1093/biolre/ioae076

Xinyue Chen, Xueguang Zhang, Ting Jiang, Wenming Xu

{"title":"Klinefelter syndrome: etiology and clinical considerations in male infertility†.","authors":"Xinyue Chen, Xueguang Zhang, Ting Jiang, Wenming Xu","doi":"10.1093/biolre/ioae076","DOIUrl":"10.1093/biolre/ioae076","url":null,"abstract":"Klinefelter syndrome (KS) is the most prevalent chromosomal disorder occurring in males. It is defined by an additional X chromosome, 47,XXY, resulting from errors in chromosomal segregation during parental gametogenesis. A major phenotype is impaired reproductive function, in the form of low testosterone and infertility. This review comprehensively examines the genetic and physiological factors contributing to infertility in KS, in addition to emergent assisted reproductive technologies, and the unique ethical challenges KS patients face when seeking infertility treatment. The pathology underlying KS is increased susceptibility for meiotic errors during spermatogenesis, resulting in aneuploid or even polyploid gametes. Specific genetic elements potentiating this susceptibility include polymorphisms in checkpoint genes regulating chromosomal synapsis and segregation. Physiologically, the additional sex chromosome also alters testicular endocrinology and metabolism by dysregulating interstitial and Sertoli cell function, collectively impairing normal sperm development. Additionally, epigenetic modifications like aberrant DNA methylation are being increasingly implicated in these disruptions. We also discuss assisted reproductive approaches leveraged in infertility management for KS patients. Application of assisted reproductive approaches, along with deep comprehension of the meiotic and endocrine disturbances precipitated by supernumerary X chromosomes, shows promise in enabling biological parenthood for KS individuals. This will require continued multidisciplinary collaboration between experts with background of genetics, physiology, ethics, and clinical reproductive medicine.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141086824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin as a natural anti-inflammatory and anti-oxidant therapy in the testis: a focus on infertility and aging†. 褪黑激素作为睾丸的天然抗炎和抗氧化疗法：聚焦不育和衰老†。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-14 DOI: 10.1093/biolre/ioae087

Mónica Beatriz Frungieri, Ricardo Saúl Calandra, María Eugenia Matzkin, Soledad Paola Rossi

{"title":"Melatonin as a natural anti-inflammatory and anti-oxidant therapy in the testis: a focus on infertility and aging†.","authors":"Mónica Beatriz Frungieri, Ricardo Saúl Calandra, María Eugenia Matzkin, Soledad Paola Rossi","doi":"10.1093/biolre/ioae087","DOIUrl":"10.1093/biolre/ioae087","url":null,"abstract":"Melatonin is a pineal hormone that regulates testicular activity (i.e., steroidogenesis and spermatogenesis) through two complementary mechanisms, indirect effects exerted via the hypothalamic-adenohypophyseal axis and direct actions that take place on the different cell populations of the male gonad. The effects of increased age on the testis and the general mechanisms involved in testicular pathology leading to infertility are still only poorly understood. However, there is growing evidence that link testicular aging and idiopathic male infertility to local inflammatory and oxidative stress events. Because literature data strongly indicate that melatonin exhibits anti-inflammatory and anti-oxidant properties, this review focuses on the potential benefits exerted by this indoleamine at testicular level in male reproductive fertility and aging. Taking into account that the effects of melatonin supplementation on testicular function are currently being investigated, the overview covers not only promising prospects but also many questions concerning the future therapeutic value of this indoleamine as an anti-aging drug as well as in the management of cases of male infertility for which there are no medical treatments currently available.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141309901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overview of the Multispecies Ovary Tissue Histology Electronic Repository†. 多物种卵巢组织组学电子储存库（MOTHER）概述。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-14 DOI: 10.1093/biolre/ioae101

Karen H Watanabe, Suzanne W Dietrich, Yian Ding, Wenli Ma, James P Sluka, Mary B Zelinski

引用次数: 0

A novel murine model mimicking male genital Neisseria species infection using Neisseria musculi†. 利用蕈样奈瑟氏菌模拟男性生殖器奈瑟氏菌感染的新型小鼠模型。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-14 DOI: 10.1093/biolre/ioae100

Emily R Bryan, Julia McRae, Vishnu Kumar, Logan K Trim, Toby I Maidment, Jacob A D Tickner, Emma L Sweeney, Elizabeth D Williams, David M Whiley, Kenneth W Beagley

{"title":"A novel murine model mimicking male genital Neisseria species infection using Neisseria musculi†.","authors":"Emily R Bryan, Julia McRae, Vishnu Kumar, Logan K Trim, Toby I Maidment, Jacob A D Tickner, Emma L Sweeney, Elizabeth D Williams, David M Whiley, Kenneth W Beagley","doi":"10.1093/biolre/ioae100","DOIUrl":"10.1093/biolre/ioae100","url":null,"abstract":"With ~78 million cases yearly, the sexually transmitted bacterium Neisseria gonorrhoeae is an urgent threat to global public health due to continued emergence of antimicrobial resistance. In the male reproductive tract, untreated infections may cause permanent damage, poor sperm quality, and subsequently subfertility. Currently, few animal models exist for N. gonorrhoeae infection, which has strict human tropism, and available models have limited translatability to human disease. The absence of appropriate models inhibits the development of vital new diagnostics and treatments. However, the discovery of Neisseria musculi, a mouse oral cavity bacterium, offers much promise. This bacterium has already been used to develop an oral Neisseria infection model, but the feasibility of establishing urogenital gonococcal models is unexplored. We inoculated mice via the intrapenile route with N. musculi. We assessed bacterial burden throughout the male reproductive tract, the systemic and tissue-specific immune response 2-weeks postinfection, and the effect of infection on sperm health. Neisseria musculi was found in penis (2/5) and vas deferens (3/5) tissues. Infection altered immune cell counts: CD19+ (spleen, lymph node, penis), F4/80+ (spleen, lymph node, epididymus), and Gr1+ (penis) compared with noninfected mice. This culminated in sperm from infected mice having poor viability, motility, and morphology. We hypothesize that in the absence of testis infection, infection and inflammation in other reproductive is sufficient to damage sperm quality. Many results herein are consistent with outcomes of gonorrhoea infection, indicating the potential of this model as a tool for enhancing the understanding of Neisseria infections of the human male reproductive tract.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0