Biology of Reproduction最新文献_第5页

Method for modeling oviduct function and impact on embryonic development†. 输卵管功能建模方法及其对胚胎发育的影响

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-10-09 DOI: 10.1093/biolre/ioag225

Kalli K Stephens, Vakil Ahmad, Maria A Silva, Makenna K Shifflett, Jiude Mao, Jason A Rizo, Mark I Hunter, Andrew M Kelleher, Wipawee Winuthayanon

{"title":"Method for modeling oviduct function and impact on embryonic development†.","authors":"Kalli K Stephens, Vakil Ahmad, Maria A Silva, Makenna K Shifflett, Jiude Mao, Jason A Rizo, Mark I Hunter, Andrew M Kelleher, Wipawee Winuthayanon","doi":"10.1093/biolre/ioag225","DOIUrl":"10.1093/biolre/ioag225","url":null,"abstract":"Direct experimental analysis of the mammalian oviduct is constrained by limited tissue access and the short lifespan of ex vivo preparations. Extracellular matrix-embedded three-dimensional epithelial organoids provide longer-term in vitro models. However, their inward-facing apical surface and the absence of supporting stromal cells limit physiological studies of the oviduct, including ciliary activity and maternal-embryonic interactions. Here, we provide a step-wise protocol detailing the generation of mouse and human oviductal assembloids in which epithelial cells form an outward-facing (apical-out) layer around a stromal core. Epithelial and stromal cells from adult mouse oviducts or human Fallopian tubes are isolated, expanded separately, and subsequently aggregated in a rotational culture system. The protocol also outlines morphological and immunostaining criteria for confirming cellular organization, whole-mount detection of external cilia, measurement of ciliary beat frequency, and co-culture of mouse assembloids with preimplantation embryos. Mouse and human assembloids retained epithelial and stromal identity and displayed cilia at the accessible outer surface. In a proof-of-concept experiment, embryos co-cultured with the assembloids developed to blastocysts at a rate similar to that of in vivo-derived blastocysts. This reductionist system provides a straightforward and tractable model to investigate oviduct physiology and embryo-maternal communication while allowing direct manipulation and observation of the epithelial interface.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OCT4 is essential for Trophectoderm Expansion in Pigs. OCT4对猪的滋养外胚层扩张至关重要。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-10-06 DOI: 10.1093/biolre/ioag218

Zicheng Qi, Jing Wu, Yuchuan Yang, Zhuang Chai, Jiaqiang Wang, Zhonghua Liu

引用次数: 0

Metagenome shotgun sequencing allows insights into the functional potential of the vaginal microbiome associated with pelvic organ prolapse in sows†. 宏基因组霰弹枪测序可以深入了解与母猪盆腔器官脱垂相关的阴道微生物群的功能潜力。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-10-06 DOI: 10.1093/biolre/ioag222

Zoë E Kiefer, Chiron J Anderson, Faith M Rahic-Seggerman, Stephan Schmitz-Esser, Jason W Ross

{"title":"Metagenome shotgun sequencing allows insights into the functional potential of the vaginal microbiome associated with pelvic organ prolapse in sows†.","authors":"Zoë E Kiefer, Chiron J Anderson, Faith M Rahic-Seggerman, Stephan Schmitz-Esser, Jason W Ross","doi":"10.1093/biolre/ioag222","DOIUrl":"https://doi.org/10.1093/biolre/ioag222","url":null,"abstract":"Pelvic organ prolapse (POP) is a leading cause for sow mortality in the United States. Recent work has evaluated biological factors associated with POP, and differences have been observed within the vaginal microbiota of sows at high risk using 16S rRNA gene amplicon sequencing. Additional functional studies are needed to better understand the relationship of dysbiosis of the vaginal microbiome with POP risk in sows. The current study's objective was to provide information about the functional potential of the sow vaginal microbiome, and to identify candidate genes and organisms that may be associated with POP. For this, metagenome shotgun sequencing was conducted on DNA extracted from 16 vaginal swab samples from late gestation sows. Of the 16 samples, 8 were from sows at high risk and 8 from sows at low risk for POP. Of the 8 samples from sows at high risk for POP four subsequently experienced POP. Subsequent contigs were annotated to generate a gene catalog of the vaginal microbiome of sows. The contigs were binned into metagenome assembled genomes (MAGs), resulting in 10 high-quality MAGs identified as Mannheimia varigena, Corynebacterium maris, Turicibacter bilis, Staphylococcus hyicus, Streptococcus dysgalactiae, Anaerococcus prevotii, Actinobacillus rossii, Prevotellaceae, Methanobrevibacter, and Veillonella caviae. MAGs classified as Streptococcus dysgalactiae and Staphylococcus hyicus contained potential virulence factors that are linked to the weakening of the connective tissue of the reproductive tract. This work provides initial insights into the functional potential of the vaginal microbial communities in late gestation sows in relation to POP and reproductive health.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Cell RNA Sequencing deciphers the imbalance of Microenvironment of the Maternal-Fetal Interface in Cesarean Scar Pregnancy. 单细胞RNA测序分析剖宫产瘢痕妊娠母胎界面微环境失衡。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-10-05 DOI: 10.1093/biolre/ioag221

Tingting Wang, Jingshu Wei, Jie Yan, Huixia Yang

{"title":"Single-Cell RNA Sequencing deciphers the imbalance of Microenvironment of the Maternal-Fetal Interface in Cesarean Scar Pregnancy.","authors":"Tingting Wang, Jingshu Wei, Jie Yan, Huixia Yang","doi":"10.1093/biolre/ioag221","DOIUrl":"https://doi.org/10.1093/biolre/ioag221","url":null,"abstract":"Cesarean scar pregnancy (CSP) has posed a serious threat to women's reproductive health. The view on its mechanism remained controversial. Here, we performed a single-cell sequencing on villi and decidua from three normal and three cesarean scar pregnancies in patients with a history of cesarean delivery. Enrichment analysis was performed to elucidate the functional roles of various cell types. Pseudotime analysis was utilized to infer the differentiation of trajectory. SCENIC provided insights into transcription regulation. CellChat was employed to explore intercellular interactions. A single-cell atlas of the maternal-fetal interface microenvironment in patients with a history of cesarean delivery was conducted to elucidate aberrant activities associated with CSP. Significant alterations were observed in trophoblast differentiation and function, including changes in angiogenesis, migration, and interactions with the extracellular matrix (ECM). A marked reduction in decidual stroma cells was observed, accompanied by abnormal ECM remodeling. Neutrophils (Neu) emerged as the principal immune cells, with elevated numbers and activation. Inflammatory pathways such as NF-κB in Neu increased. Cellular interactions between stroma cells and trophoblasts were mainly COLLAGEN, FN1, and LAMININ, and between immune cells and trophoblasts were VISFATIN and CXCL. This comprehensive single-cell analysis has elucidated the cellular heterogeneity at the maternal-fetal interface in patients with a history of cesarean delivery and shed light on distinct cellular abnormalities associated with CSP.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-omic profiling defines subtype-specific extracellular vesicle signatures in early human pregnancy. 多组学分析定义了人类妊娠早期亚型特异性细胞外囊泡特征。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-10-05 DOI: 10.1093/biolre/ioag220

Kobe A Abney, Taylor Hollingsworth, Alexandra Schneider, Elizabeth M Brown, Hossein Fazelinia, Lynn A Spruce, Rita Leite, Samuel Parry, Nadav Schwartz, Colin C Conine, Rebecca A Simmons

{"title":"Multi-omic profiling defines subtype-specific extracellular vesicle signatures in early human pregnancy.","authors":"Kobe A Abney, Taylor Hollingsworth, Alexandra Schneider, Elizabeth M Brown, Hossein Fazelinia, Lynn A Spruce, Rita Leite, Samuel Parry, Nadav Schwartz, Colin C Conine, Rebecca A Simmons","doi":"10.1093/biolre/ioag220","DOIUrl":"https://doi.org/10.1093/biolre/ioag220","url":null,"abstract":"Early human pregnancy (11-15 weeks) is a critical period when placental dysfunction begins but remains clinically undetectable, preventing early intervention for pregnancy complications. This highlights a pressing need for circulating biomarkers that can signal the onset of a complication. Circulating extracellular vesicles (EVs) carry bioactive cargo that reflect originating cell activity in real-time, positioning them as early biomarkers of pregnancy complications. While pregnancy studies have identified plasma EV cargo alterations across complications, studying small EVs alone-the field standard-or bulk EVs together obscures pathological changes that may manifest preferentially in one population. To address this, we performed the first multi-omic comparison of small and large plasma EVs from healthy pregnancies at 11-13 weeks (n=10) to establish subtype-specific cargo differences and provide a reference for normal pregnancy EV biology. We quantified mitochondrial DNA (mtDNA), performed transcriptomics (mRNAs, miRNAs) and proteomics to define small and large EV signatures. Large EVs were preferentially enriched for mitochondrial cargo including DNA, transcripts, and proteins as well as hypoxia response, endocrine signaling, and VEGF signaling related transcripts and proteins. In contrast, small EVs were enriched for canonical EV markers and basement membrane proteins. Additionally, we found that EV miRNA composition was similar across EV subtypes with only 7 miRNAs differentially abundant between small and large EVs. These distinct small and large EV cargo signatures underscore the importance of profiling both populations for liquid biopsy potential. Together these data provide a baseline for future studies linking EV signature alterations to placental dysfunction and adverse pregnancy outcomes.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reproductive phenotypes and transcriptomic responses associated with Trpv1 deficiency in mice. 小鼠Trpv1缺乏与生殖表型和转录组反应相关。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-10-01 DOI: 10.1093/biolre/ioag217

Penghao Li, Yingyu Luo, Tao Wang, Diyan Li

{"title":"Reproductive phenotypes and transcriptomic responses associated with Trpv1 deficiency in mice.","authors":"Penghao Li, Yingyu Luo, Tao Wang, Diyan Li","doi":"10.1093/biolre/ioag217","DOIUrl":"https://doi.org/10.1093/biolre/ioag217","url":null,"abstract":"The transient receptor potential cation channel subfamily V member 1 (Trpv1) is a polymodal sensor of noxious stimuli, including heat and pain. However, its contribution to mammalian reproductive physiology remains incompletely understood. Here, we evaluated reproductive phenotypes and transcriptomic responses in a Trpv1-knockout mouse model under normal and heat-stress conditions. Vaginal-smear profiling showed altered estrous cyclicity in Trpv1-deficient females. Bulk RNA-seq and Smart-seq2 analyses identified tissue- and condition-dependent gene-expression changes associated with Trpv1 deficiency, including extracellular-matrix-related transcriptional signatures in heat-stressed oocytes. In males, Trpv1 deficiency was associated with altered sperm-motility measurements under heat stress, changes in steroidogenesis-related transcripts, and testicular stress-response signatures. These findings identify candidate molecular pathways associated with Trpv1 deficiency and thermal challenge. Further studies, including mating, fertilization, embryo-development, protein-level, and cellular functional assays, are required to determine the consequences for gamete competence and fertility.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induced vs Spontaneous Ovulation: Integrating KNDy Circuitry, Sensory Cues, and Ecological Context. 诱导与自发排卵：整合KNDy电路，感觉线索和生态背景。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-10-01 DOI: 10.1093/biolre/ioag219

An An Xie, Aaron J Hsueh, An An Xie, Aaron J Hsueh

{"title":"Induced vs Spontaneous Ovulation: Integrating KNDy Circuitry, Sensory Cues, and Ecological Context.","authors":"An An Xie, Aaron J Hsueh, An An Xie, Aaron J Hsueh","doi":"10.1093/biolre/ioag219","DOIUrl":"https://doi.org/10.1093/biolre/ioag219","url":null,"abstract":"Ovulation links folliculogenesis to fertilization and pregnancy, yet vertebrates have evolved contrasting ovulatory strategies that reflect ecological and social pressures. Spontaneous ovulators rely on internally generated cyclic signals, whereas induced ovulators couple follicular rupture to external stimuli such as copulation or social cues. Despite these differences, both strategies converge on a conserved hypothalamic-pituitary-ovarian cascade in which a GnRH-driven preovulatory LH surge coordinates oocyte maturation, follicle rupture, and corpus luteum formation. Arcuate Kisspeptin/Neurokinin B/Dynorphin (KNDy) neurons function as the GnRH pulse generator, while anteroventral periventricular nucleus/Preoptic Area (AVPV/POA) neurons are the GnRH surge generator by integrating estradiol-mediated positive feedback to gate the timing and amplitude of this surge. This review aims to contrast spontaneous and induced ovulation across vertebrates, emphasizing how both strategies converge on a common final pathway. The review then examines how diverse sensory and environmental cues are wired into this core axis in taxa such as camelids, rabbits, cats, honeycomb groupers, most birds, and zebrafish. Finally, ecological and social contexts are considered, highlighting how ovulation mode shapes paternity monopolization, mating systems, and the adaptive balance between male reproductive success and female control over fertilization.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Irisin as a metabolic regulator of male reproduction. 鸢尾素作为雄性生殖的代谢调节剂。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-10-01 DOI: 10.1093/biolre/ioag183

Iqra Batool, Eduardo Rodrigues Pessoa, Elzania Sales Pereira, Denise Damasceno Guerreiro, Arlindo Alencar Moura

{"title":"Irisin as a metabolic regulator of male reproduction.","authors":"Iqra Batool, Eduardo Rodrigues Pessoa, Elzania Sales Pereira, Denise Damasceno Guerreiro, Arlindo Alencar Moura","doi":"10.1093/biolre/ioag183","DOIUrl":"https://doi.org/10.1093/biolre/ioag183","url":null,"abstract":"Obesity and metabolic syndrome are major contributors to male infertility. These conditions disrupt the hypothalamic-pituitary-gonadal (HPG) axis and compromise testicular steroidogenesis and spermatogenesis through mechanisms that include altered aromatase activity, insulin resistance, chronic inflammation, and oxidative stress. Irisin, an exercise-induced myokine, has emerged as a metabolic signal with potential effects on male reproductive function. This review summarizes irisin biology, proposed mechanisms of action, and putative functions of irisin in male reproduction, with emphasis on obesity-associated fertility impairment. Studies in high-fat diet-induced obese rodent models indicate that exogenous irisin can improve metabolic homeostasis, restore testosterone production, increase expression of key steroidogenic enzymes, and mitigate obesity-associated reductions in spermatogenesis and sperm quality. These effects are commonly attributed to anti-inflammatory, antioxidant, and cytoprotective actions, as well as modulation of cellular energy metabolism through AMP-activated protein kinase and mitogen-activated protein kinase signalling. Irisin has also been reported to influence central neuroendocrine pathways involved in gonadotropin regulation and to improve sexual behaviour in rodent models of metabolic- and antidepressant-induced sexual dysfunction. However, the physiological relevance of irisin in men's reproductive physiology remains incompletely defined. Clinical evidence is largely associative, and data on irisin receptor expression and downstream signalling in the male reproductive tract are limited. Key gaps also include the regulation of irisin secretion and potential tissue-specific actions across stages of male reproductive development. Translational studies are needed to clarify irisin's actions within the HPG axis and gonads, and to evaluate its potential to mitigate obesity-related male reproductive dysfunction.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neddylation inhibition alleviates polycystic ovary syndrome by regulating granulosa cell function and androgen synthesis. 类泛素化抑制通过调节颗粒细胞功能和雄激素合成减轻多囊卵巢综合征。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-30 DOI: 10.1093/biolre/ioag193

Xiaowei Qin, Ting Luo, Haixia Song, Yunyun Liu, Xumin Zhang, Qin Qin, Jianrong Liu

{"title":"Neddylation inhibition alleviates polycystic ovary syndrome by regulating granulosa cell function and androgen synthesis.","authors":"Xiaowei Qin, Ting Luo, Haixia Song, Yunyun Liu, Xumin Zhang, Qin Qin, Jianrong Liu","doi":"10.1093/biolre/ioag193","DOIUrl":"https://doi.org/10.1093/biolre/ioag193","url":null,"abstract":"Polycystic ovary syndrome (PCOS) is a common and complex endocrine and metabolic disorder whose etiology has not yet been fully elucidated. Emerging research has revealed an association between PCOS pathogenesis and post-translational modifications of proteins. However, the effect of neddylation on PCOS pathogenesis has not been investigated. In this study, key proteins of the neddylation pathway (Nedd8, neddylation-activating enzyme (NAE), and Cullin-1/2/3) were significantly upregulated in the granulosa cells of patients with PCOS; this was accompanied by enhanced apoptosis (increased Bax and Caspase-3, decreased Bcl-2) and elevated expression of the androgen synthesis-related proteins, namely: androgen receptor (AR) and Cytochrome P450 Family 17 Subfamily A Member 1(CYP17A1). In the in vitro DHT-induced PCOS KGN cell model, key neddylation pathway proteins (Nedd8, NAE, and Cullin-1) were upregulated. Overexpression of Nedd8 and inhibition of neddylation by MLN4924 promoted apoptosis. However, inhibition of neddylation by MLN4924 reversed AR/CYP17A1 protein expression. In a rat model of PCOS, MLN4924 treatment significantly suppressed the expression of Nedd8, NAE, and Cullin-1/2/3 in the ovarian tissue, restored estrous cycles, alleviated ovarian morphological abnormalities (characterized by increased corpora lutea and reduced cystic follicles), and decreased serum testosterone and LH levels. MLN4924 significantly promoted ovarian granulosa cell apoptosis, but reduced the expression of androgen synthesis-related proteins. Together, these findings indicate the unrecognized role of neddylation in PCOS, while highlighting that MLN4924 represents a potential therapeutic approach for PCOS treatment.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149079469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological significance of centromeric satellite asymmetry in female meiosis. 雌性减数分裂中着丝体不对称的生物学意义。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-28 DOI: 10.1093/biolre/ioag216

Deepanshu Kumar, Michael A Lampson

引用次数: 0