Biology of Reproduction最新文献_第5页

Granulosa cell death is a significant contributor to DNA-damaging chemotherapy-induced ovarian insufficiency†. 颗粒细胞死亡是化疗诱导的dna损伤性卵巢功能不全的重要因素。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-05-13 DOI: 10.1093/biolre/ioae181

Mahiru Kawano, Jennifer McKey, Iordan S Batchvarov, Blanche Capel

{"title":"Granulosa cell death is a significant contributor to DNA-damaging chemotherapy-induced ovarian insufficiency†.","authors":"Mahiru Kawano, Jennifer McKey, Iordan S Batchvarov, Blanche Capel","doi":"10.1093/biolre/ioae181","DOIUrl":"10.1093/biolre/ioae181","url":null,"abstract":"Typically, DNA-damaging chemotherapy (CTx) regimens have a gonadotoxic effect and cause premature ovarian insufficiency (POI), characterized by infertility and estrogen deficiency. However, whether loss of granulosa cells killed directly by CTx contributes significantly to POI has not been determined. To address this issue, we used a previously established mouse model of CTx-induced POI. The alkylating drugs Busulfan (8.75 mg/kg) and Cyclophosphamide (100 mg/kg) were administered to 8-week-old FVB female mice by intraperitoneal (IP) injection three times at 48-h intervals, after which ovarian tissues were harvested and examined by immunofluorescence. The number of primordial follicles was significantly reduced at day (d)6, whereas the number of growing follicles was relatively unchanged. CTx led to DNA double strand breaks in both oocytes and granulosa cells based on the presence of γH2AX foci. However, markers of apoptosis predominantly labeled granulosa cells in growing follicles. We next examined the effect of inhibiting apoptosis in growing granulosa cells by generating Bak-/-Baxfx/fx; Cyp19a1Cre transgenic mice. On d10 after the first CTx, Bak-/-Baxfx/fx; Cyp19a1Cre ovaries had fewer apoptotic granulosa cells and more surviving follicles than controls. Furthermore, Bak-/-Baxfx/fx; Cyp19a1Cre mice showed better fertility than controls after CTx. Our data suggest that granulosa cell death is a significant contributor to follicle depletion and fertility loss after Cyclophosphamide and Busulfan.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"906-915"},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single cell multiome analysis of the bovine placenta identifies gene regulatory networks in trophoblast differentiation†. 牛胎盘单细胞多组分析鉴定滋养细胞分化的基因调控网络。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-05-13 DOI: 10.1093/biolre/ioaf036

Kimberly M Davenport, Makenzie T Lowke, M Sofia Ortega, Andrew M Kelleher, Wesley C Warren, Thomas E Spencer

{"title":"Single cell multiome analysis of the bovine placenta identifies gene regulatory networks in trophoblast differentiation†.","authors":"Kimberly M Davenport, Makenzie T Lowke, M Sofia Ortega, Andrew M Kelleher, Wesley C Warren, Thomas E Spencer","doi":"10.1093/biolre/ioaf036","DOIUrl":"10.1093/biolre/ioaf036","url":null,"abstract":"A central determinant of successful reproduction is pregnancy establishment and maintenance that relies on proper development of the conceptus (embryo/fetus and associated extraembryonic membranes including the placenta). Pregnancy loss in cattle can be caused by inadequate development and differentiation of the placenta. However, the cellular and molecular mechanisms regulating bovine placenta development and, particularly, trophoblast differentiation are not well understood. Recent single-cell RNA-seq analyses revealed dynamic changes in cell populations and gene expression patterns during bovine placental development. Here, the chromatin accessibility landscape across diverse cell populations was determined in the developing (Day 40) and mature (Day 170) bovine placenta using the 10X Genomics multiome (snRNA-seq and snATAC-seq) platform. Analyses revealed distinct trophoblast, mesenchyme, endothelial, immune, and epithelial cell populations characterized by unique gene expression and chromatin accessibility signatures. ATAC-seq peaks defined open chromatin regions, facilitating the identification of transcription factor binding sites and candidate gene regulatory networks involved with trophoblast differentiation. Several transcription factors, known for their involvement in trophoblast differentiation in other mammalian species, were identified as candidate regulators of uninucleate to binucleate trophoblast differentiation. This study adds to our foundational understanding of gene regulation and expression in the placenta, offering insights into the mechanisms governing pregnancy loss in cattle.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"955-968"},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early identification of bovine pregnancy status and embryonic mortality†. 早期鉴定牛妊娠状态和胚胎死亡率†。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-05-13 DOI: 10.1093/biolre/ioaf066

Jeanette V Bishop, Aydin Guzeloglu, Tom Scheller, Joshua J Docheff, Carolina L Gonzalez-Berrios, Hana Van Campen, Terry M Nett, Abigail L Zezeski, Thomas W Geary, William W Thatcher, Thomas R Hansen

{"title":"Early identification of bovine pregnancy status and embryonic mortality†.","authors":"Jeanette V Bishop, Aydin Guzeloglu, Tom Scheller, Joshua J Docheff, Carolina L Gonzalez-Berrios, Hana Van Campen, Terry M Nett, Abigail L Zezeski, Thomas W Geary, William W Thatcher, Thomas R Hansen","doi":"10.1093/biolre/ioaf066","DOIUrl":"10.1093/biolre/ioaf066","url":null,"abstract":"Bovine interferon-tau (bIFNT) is produced by the trophectoderm cells in the bovine conceptus as early as Day 12 following fertilization. It was hypothesized that IFNT detection in blood, milk, and/or cervical secretions could be used to diagnose pregnancy in lactating cows. Recombinant bovine (rb) IFNT was generated to produce goat and rabbit anti-rbIFNT polyclonal antibodies, and an enzyme-linked immunosorbent assay (ELISA) was developed for bIFNT using these reagents. The IFNT ELISA did not cross-react with other type I or II IFNs and had a limit of detection of 50-100 pg/ml. The IFNT ELISA detected IFNT in external ostium (os) cervical swabs from Days 15 to 25 post-AI, but did not detect IFNT in serum, plasma, or milk. The time for most accurately detecting IFNT in cervical fluid was Days 16-19 after AI. A custom bovine swab device used to collect cervical secretions reduced false-negative rates to 5.5% (94.5% sensitivity) in dairy cows on Day 17 and 0% to 3.4% (100% and 96.6% sensitivity) in beef cows on Days 18 or 16, respectively. In summary, the detection of IFNT in cervical fluid by ELISA provides an accurate indication of pregnancy status in lactating dairy cows. Early identification of the non-pregnant cow allows re-insemination on Day 21 compared to waiting until ultrasound (US) on Day ~32-39. In addition, the detection of IFNT on Day 17 followed by the loss of pregnancy detected by US on Day 32 provides a novel research tool for studying pregnancy loss caused by embryonic mortality.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"981-995"},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppression of FOXO3 by BMP signaling contribute to the different primordial germ cell proliferation between layers and broilers†. BMP信号对FOXO3的抑制与蛋鸡和肉鸡原始生殖细胞增殖的差异有关。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-05-13 DOI: 10.1093/biolre/ioaf037

Yuxiao Ma, Lu Meng, Jiahui Wei, Wenhui Wu, Yun Zhang, Xuzhao Wang, Xiaotong Guo, Feiyi Wang, Yong Mao, Guiyu Zhu

{"title":"Suppression of FOXO3 by BMP signaling contribute to the different primordial germ cell proliferation between layers and broilers†.","authors":"Yuxiao Ma, Lu Meng, Jiahui Wei, Wenhui Wu, Yun Zhang, Xuzhao Wang, Xiaotong Guo, Feiyi Wang, Yong Mao, Guiyu Zhu","doi":"10.1093/biolre/ioaf037","DOIUrl":"10.1093/biolre/ioaf037","url":null,"abstract":"Although all domestic chicken breeds originate from their wild relatives, the red junglefowl, they have been selectively bred for high yields in either egg or meat production, or both. Some breeds are highly efficient in egg production, while others perform poorly, due to long-term selection aimed for different purposes. Female primordial germ cells (PGCs) are the precursors of eggs and the population size of PGCs will ultimately determine ovarian reserve of hens. In this study, we observed that the layers exhibit greater proliferation capacity and a higher number of PGCs in the gonad compared to the broilers before meiosis. By comparing the PGC transcriptomes between layers and broilers, we identified potential genes that regulate cell proliferation. We further confirmed that FOXO3 expression is higher in broilers, where it inhibits the PGC proliferation both in vivo and in vitro. However, in layers, the upstream BMP signaling stimulate the phosphorylation of AKT and suppress FOXO3 activity. Consequently, the elevated BMP signaling and reduced FOXO3 co-operatively promote more robust PGC proliferation in layers compared to broilers and result in a greater number of PGCs in layers. Our data not only reveal molecular mechanisms underlying PGC growth in the ovary, but also provide new clues to improve the laying performance in chicken.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"942-954"},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IDO can improve ovarian function in premature ovarian insufficiency via AhR and regulatory T cells. IDO可通过AhR和调节性T细胞改善卵巢功能不全患者的卵巢功能。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-05-13 DOI: 10.1093/biolre/ioaf102

Anchun Hu, Yanli Mu, Guanyou Huang, Zhongan Wang, Shuyun Zhao, Wenchi Xu, Panpan Chen, Xin Guo

{"title":"IDO can improve ovarian function in premature ovarian insufficiency via AhR and regulatory T cells.","authors":"Anchun Hu, Yanli Mu, Guanyou Huang, Zhongan Wang, Shuyun Zhao, Wenchi Xu, Panpan Chen, Xin Guo","doi":"10.1093/biolre/ioaf102","DOIUrl":"https://doi.org/10.1093/biolre/ioaf102","url":null,"abstract":"Background: Premature ovarian insufficiency (POI) is the loss of ovarian function among women <40 years of age, and immune disorders play a critical role in POI development. Indoleamine 2,3-dioxygenase (IDO) catalyses tryptophan metabolism via the kynurenine pathway and plays a key role in preventing and treating immune-related diseases.Methods: Human ovarian granulosa cells (hGCs) were collected via the density gradient method, pZP3 was used to establish an immune POI mouse model, and an AAV vector carrying IDO1 (IDO-AAV) was injected into mouse ovaries to induce IDO overexpression. Ovarian function was measured by the oestrous cycle, serum AMH concentration, degree of ovarian fibrosis and number of follicles.Findings: IDO protein levels and mRNA expression in hGCs were lower in the POI group than in the control group (P < 0.05). Both the ovarian function and IDO levels in the POI + Water group and the POI + Glu group were significantly lower than those in the control group. In POI model mice injected with IDO-AAV, ovarian function and the CD4 + CD25 + Foxp3+ regulatory T (Treg) cell proportion were increased compared with those in mice injected with the natural control AAV. The FoxP3 mRNA expression level in Treg cells was positively correlated with the IDO mRNA expression level, whereas the RORγt mRNA expression level in Th17 cells was negatively correlated with the IDO mRNA expression level, further suggesting that IDO may be related to Treg and Th17 cells through AhR and subsequently regulate immunity and ovarian function.Conclusion: Increasing ovarian IDO levels in POI mice improved ovarian function.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disruption of oocyte SUMOylation impacts critical regulatory processes during folliculogenesis in mice†. 卵母细胞sumo酰化的破坏影响小鼠卵泡发生过程中的关键调控过程。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-05-13 DOI: 10.1093/biolre/ioaf035

Zian Liao, Tessa E Steenwinkel, Bruno Moscoso, Ernesto Salas, Bethany K Patton, Amanda Rodriguez, Anna Malovannaya, Stephanie A Pangas

{"title":"Disruption of oocyte SUMOylation impacts critical regulatory processes during folliculogenesis in mice†.","authors":"Zian Liao, Tessa E Steenwinkel, Bruno Moscoso, Ernesto Salas, Bethany K Patton, Amanda Rodriguez, Anna Malovannaya, Stephanie A Pangas","doi":"10.1093/biolre/ioaf035","DOIUrl":"10.1093/biolre/ioaf035","url":null,"abstract":"The conjugation of small ubiquitin-like modifiers (SUMO) to target proteins, known as SUMOylation, plays a crucial role in regulating protein homeostasis, activity, interaction with other proteins, and subcellular localization. Loss of SUMOylation in nongrowing oocytes by conditional deletion of the E2 SUMO conjugating enzyme, Ube2i, at the primordial follicle stage leads to female sterility due to complex changes in oocyte development, including altered folliculogenesis, defective meiotic progression, and premature loss of the ovarian reserve. In this study, proteomics was used to compare control and Ube2i conditional knockout ovaries during the first wave of folliculogenesis to identify key differences that may drive the premature follicle loss phenotype. Label-free mass spectrometry results showed that 238 proteins were significantly altered more than 2-fold (p < 0.05). Proteins upregulated in the Ube2i conditional knockout ovaries included those involved in mRNA splicing and WNT signaling, while those downregulated were related to metabolism, mitochondria, and the maternal effect proteins NLRP2 and NLRP9B. The majority of differentially expressed proteins showed no change by transcriptome analysis, indicating protein level regulation and revealing potential SUMOylation targets with necessary roles in oocyte and follicle development.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"932-941"},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reactive oxygen and nitrogen species: multifaceted regulators of ovarian activity†. 活性氧和活性氮：卵巢活动的多方面调节因子。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-05-13 DOI: 10.1093/biolre/ioaf032

Jiří Bezdíček, Jana Sekaninová, Martina Janků, Alexander Makarevič, Lenka Luhová, Linda Dujíčková, Marek Petřivalský

{"title":"Reactive oxygen and nitrogen species: multifaceted regulators of ovarian activity†.","authors":"Jiří Bezdíček, Jana Sekaninová, Martina Janků, Alexander Makarevič, Lenka Luhová, Linda Dujíčková, Marek Petřivalský","doi":"10.1093/biolre/ioaf032","DOIUrl":"10.1093/biolre/ioaf032","url":null,"abstract":"Reactive oxygen and nitrogen species are essential components of diverse intracellular signaling pathways. In addition to their involvement in apoptosis, reactive oxygen and nitrogen species are crucial in the regulation of multiple developmental and physiological processes. This review aims to summarize their role in the regulation of key ovarian stages: ovulation, maturation and postovulatory ageing of the oocyte, and the formation and regression of the corpus luteum. At the cellular level, a mild increase in reactive oxygen and nitrogen species is associated with the initiation of a number of regulatory mechanisms, which might be suppressed by increased activity of the antioxidant system. Moreover, a mild increase in reactive oxygen and nitrogen species has been linked to the control of mitochondrial biogenesis and abundance in response to increased cellular energy demands. Thus, reactive oxygen and nitrogen species should also be perceived in terms of their positive role in cellular signaling. On the other hand, an uncontrolled increase in reactive oxygen species production or strong down-regulation of the antioxidant system results in oxidative stress and damage of cellular components associated with ovarian pathologies and ageing. Similarly, the disturbance of signaling functions of reactive nitrogen species caused by dysregulation of nitric oxide production by nitric oxide synthases in ovarian tissues interferes with the proper regulation of physiological processes in the ovary.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"789-806"},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in uterine and serum metabolome associated with clinical cure failure of metritis in dairy cows†. 奶牛子宫及血清代谢组差异与子宫炎临床治疗失败相关。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-05-13 DOI: 10.1093/biolre/ioaf038

Frederico Narciso de Souza Pereira, Aline Martelo Pereira, Klibs Neblan Galvão, Rafael Sisconeto Bisinotto, Caio Cesar Figueiredo

{"title":"Differences in uterine and serum metabolome associated with clinical cure failure of metritis in dairy cows†.","authors":"Frederico Narciso de Souza Pereira, Aline Martelo Pereira, Klibs Neblan Galvão, Rafael Sisconeto Bisinotto, Caio Cesar Figueiredo","doi":"10.1093/biolre/ioaf038","DOIUrl":"10.1093/biolre/ioaf038","url":null,"abstract":"This study investigated differences in uterine and serum metabolome associated with clinical cure failure of metritis in dairy cows. Metritis was diagnosed in lactating Holstein cows from two Florida dairies and defined by the presence of fetid, watery, reddish-brown vaginal discharge from 4 to 12 days postpartum (dpp). Cows with metritis (n = 24) were paired with cows without metritis of similar parity and dpp (n = 24). On the day of metritis diagnosis (day 0), all cows with metritis received antimicrobial therapy. The continued presence of the fetid, watery, reddish-brown discharge on day 5 (n = 16) was defined as clinical cure failure, whereas clinical cure was defined by its absence (n = 8). Metabolome analyses of uterine lavage (days 0 and 5) and serum samples (day 0) were conducted using untargeted gas chromatography time-of-flight mass spectrometry. Normalized data were analyzed using partial least squares-discriminant analysis and ANOVA, adjusting P-values for multiple comparisons. Differences in the uterine metabolome on day 0 associated with clinical cure failure were linked to carbohydrate, amino acid, and lipid metabolism. Greater concentrations of arachidonic acid, ribose, and glutaric acid were associated with clinical cure failure, suggesting a greater degree of tissue lesion and inflammation. No differences in the serum metabolome were associated with cure failure. No differences in uterine metabolome were associated with clinical cure failure on day 5. The findings suggest that clinical cure failure is associated with a greater uterine inflammatory process that did not persist until cure assessment day.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"858-866"},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal probiotic supplementation and effects on the fetal placenta. 母体益生菌补充及其对胎儿胎盘的影响。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-05-13 DOI: 10.1093/biolre/ioaf041

Rosalind T B Herrington, David T Ellenberger, Cheryl S Rosenfeld

{"title":"Maternal probiotic supplementation and effects on the fetal placenta.","authors":"Rosalind T B Herrington, David T Ellenberger, Cheryl S Rosenfeld","doi":"10.1093/biolre/ioaf041","DOIUrl":"10.1093/biolre/ioaf041","url":null,"abstract":"Increasing number of pregnant women are consumi ng probiotics to promote their own health and that of their unborn fetuses. Such supplements are presumed to be safe for pregnant mothers and their unborn offspring. For pregnant mothers, such bioactive compounds might lower the risk of constipation, diarrhea, other gastrointestinal conditions, and pre-term birth, and prevent adverse pregnancy outcomes, including gestational diabetes mellitus and depression/anxiety. More research is needed to examine potential safety of probiotic consumption during pregnancy and long-term health consequences to offspring. The conceptus can also be indirectly affected by maternal probiotic supplementation through microorganism production of bioactive compounds. The placenta is in direct communication with the underlying uterine tissue. Thus, compounds in the maternal blood can easily transfer across the placenta and impact this hormonally sensitive organ. Select studies suggest that disruptions to the maternal microbiome dramatically affect the placenta. In the current review, we will therefore consider the evidence to date of how maternal probiotic supplementation affects the placenta. Three potential mechanisms we will explore include the possibility that maternal probiotic supplementation might impact the putative placenta microbiome. The second potential mechanism we will consider is that maternal probiotic consumption alters bacterial-derived metabolites, including short-chained fatty acids, polyamines, Vitamin B9, and Vitamin B12. The third potential mechanism to be discussed is that such supplements affect maternal and placental immune responses. Before probiotics are promoted for healthy pregnant women and those with gestational disorders, more studies, including those examining the effects on the placenta, are essential.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"807-823"},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purinergic system molecules: expression and regulation of enzymes, transporters, and adenosine triphosphate receptors and the role of adenosine triphosphate at the maternal-conceptus interface in pigs†. 嘌呤能系统分子：酶、转运体和ATP受体的表达和调控以及ATP在猪母系-胎界面的作用。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-05-13 DOI: 10.1093/biolre/ioaf028

Yugyeong Cheon, Inkyu Yoo, Soohyung Lee, Eunhyeok Choi, Seonghyun Kim, Hakhyun Ka

{"title":"Purinergic system molecules: expression and regulation of enzymes, transporters, and adenosine triphosphate receptors and the role of adenosine triphosphate at the maternal-conceptus interface in pigs†.","authors":"Yugyeong Cheon, Inkyu Yoo, Soohyung Lee, Eunhyeok Choi, Seonghyun Kim, Hakhyun Ka","doi":"10.1093/biolre/ioaf028","DOIUrl":"10.1093/biolre/ioaf028","url":null,"abstract":"The purinergic system is composed of purine nucleotides, enzymes, transporters, and receptors and is involved in a variety of physiological processes in the body, including development, metabolism, immunity, tumorigenesis, and reproduction. The importance of the purinergic system for embryo implantation in the endometrium and in pathophysiological conditions has been shown in some species. However, the expression, regulation, and function of purinergic system molecules at the maternal-conceptus interface in pigs is not fully understood. Therefore, we determined the expression of purinergic system molecules in the endometrium during the estrous cycle and pregnancy and in the conceptus and chorioallantoic tissues during pregnancy in pigs. The expression of many purinergic system molecules in the endometrium changed dynamically during pregnancy, with the expression of several components in the endometrium greater on day 15 of pregnancy than day 15 of the estrous cycle, and it was regulated by conceptus-derived interleukin-1beta and interferon-gamma. Purinergic system molecules were also expressed in conceptuses during early pregnancy and in chorioallantoic tissues during mid- to term pregnancy. Furthermore, adenosine triphosphate, a major purinergic signaling molecule, increased migration of endometrial epithelial and conceptus trophectoderm cells, the expression of prostaglandin synthetic enzymes, and the secretion of prostaglandin F2alpha in endometrial epithelial cells in vitro. These data suggest that the purinergic system molecules expressed in the endometrium, conceptus, and chorioallantoic tissues might play an important role in the establishment and maintenance of pregnancy by regulating various cellular functions at the maternal-conceptus interface in pigs.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"840-857"},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0