Biology of Reproduction最新文献

{"title":"Relationships of phototextural characteristics of ovine presumptive zygotes to their developmental potential in vitro†.","authors":"Jakub Bartlewski, Nicole Zorzan Oliveira, Karolina Fryc, Maciej Murawski, Bahareh Ahmadi, Pawel M Bartlewski","doi":"10.1093/biolre/ioaf135","DOIUrl":"https://doi.org/10.1093/biolre/ioaf135","url":null,"abstract":"<p><p>In vitro embryo production (IVP) has widespread implications for animal husbandry and conservation programs. The overarching goal of achieving pregnancy with IVP systems calls for accurate methods of embryo viability screening. The present study set out to examine whether phototextural attributes of ovine presumptive zygotes were reliable markers of embryo developmental competence in vitro. Sheep oocytes were obtained post-mortem from nine cycling Polish Longwool ewes using the ovarian cutting and sieving method. Following in vitro maturation of oocytes and their fertilization with fresh ram semen, the development of embryos (n = 37) was monitored using time-lapse imaging. The embryos were retrospectively classified as non-arresting (attaining the blastocyst stage; n = 6) or arresting (non-dividing presumptive zygotes or embryos arresting before the 7th mitotic division; n = 31). Commercially available image analytical software ImageProPlus® was used to generate bitmaps of the regions of interest comprising zygotic cytoplasm. Subsequently, a proprietary computer program r-Algo 2.0 was used to determine if there existed clusters of pixels for which first-order phototextural characteristics of presumptive zygotes' (t0) cytoplasm (i.e. mean pixel intensity (MPI), heterogeneity (MPH), and concentration (MPC)) differed significantly between the two subsets of embryos. Within the algorithmically identified pixel intensity ranges, future non-arresting embryos at t0 exceeded their arresting counterparts in MPI (pixel range of 73-75; 74.06 ± 0.01 vs. 73.98 ± 0.008; P = 0.0007) and MPC (pixel range: 105-106; 1.25 ± 0.22% vs. 0.56 ± 0.07%; P = 0.0004) but had lower mean MPH (pixel range: 36-42; 1.81 ± 0.04 vs. 1.97 ± 0.02; P = 0.0005). Computerized segmentation of ovine zygotic microphotographs is a promising non-invasive method to predict their developmental potential in vitro.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oocyte transport against fluid flow to the fertilization site in mice: contributions of cilia beating and peristalsis†.","authors":"Toshiaki Hino","doi":"10.1093/biolre/ioaf139","DOIUrl":"https://doi.org/10.1093/biolre/ioaf139","url":null,"abstract":"<p><p>The transport of ovulated oocytes to the site of fertilization involves two main processes, the initial collection of the oocyte by the fimbria and its subsequent transport through the upper ampulla. These are crucial events preceding fertilization. Around ovulation, the oviduct exhibits active fluid secretion, peristaltic movements, and ciliary beating, all of which are believed to be involved in oocyte transport. However, their specific contributions require further clarification. In this study, we investigated how these three factors influence oocyte transport to the fertilization site in the oviduct in vivo. The oviduct of anesthetized mice was installed in a fluid-circulating chamber. By introducing fixed and stained cumulus-oocyte complexes (COCs) into the fimbria and injecting a small amount of ink into the oviduct lumen, we monitored oocyte transport and fluid dynamics. Interestingly, while oviduct fluid flowed toward the fimbria, the COC moved in the opposite direction to reach the site of fertilization. Inhibiting ciliary beating disrupted both the collection (or \"pickup\") and transport of the oocyte, whereas inhibiting peristalsis had no immediate impact on these processes. However, extended inhibition of peristalsis resulted in impaired oocyte transport. Under these conditions, fluid accumulated, and the oviduct lumen expanded, disrupting the intimate contact between the COC and the cilia. These findings indicate that ciliary beating, rather than fluid flow or peristalsis, propels the COC against the fluid flow toward the fertilization site. In addition, peristalsis maintains the luminal conditions required for effective transmission of ciliary propulsion to the COC.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144483104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying sex-chromosome-linked determinants of sexual differentiation in a WZ-ZZ sex inheritance crustacean†.","authors":"Melody Wahl, Yaniv Borojovich, Tom Levy, Noa Asculai, Rivka Manor, Barak Rotblat, Liron Levin, Vered Chalifa-Caspi, Eliahu D Aflalo, Amir Sagi","doi":"10.1093/biolre/ioaf138","DOIUrl":"https://doi.org/10.1093/biolre/ioaf138","url":null,"abstract":"<p><p>Sex determination triggers transcriptional cascades, leading to the development of male or female phenotypes. The aim of this study was to shed light on the linkage between sex determination and sexual differentiation by identifying key W/Z-linked elements that control sexual differentiation in crustaceans with the WZ-ZZ mode of sex inheritance. To this end, we leveraged our previous studies on the insulin-like androgenic gland (IAG) hormone, which serves as a master switch - termed the \"IAG switch\" - in the freshwater prawn Macrobrachium rosenbergii, in which manipulations of the IAG-switch afforded functional WZ males, ZZ females, and even WW males and females. To uncover the link between sex determination and sexual differentiation, males and females lacking either the W or the Z chromosome were used to establish a transcriptomic library of early developmental stages (embryo, larva and post-larva), followed by clustering analysis of differentially expressed genes. Mapping of these genes to the M. rosenbergii sex chromosomes yielded W/Z-linked candidates with a male-biased expression pattern culminating at day 10 post larvae (PL10), which marks the phenotypic sexual differentiation stage. Among these genes, two leading candidates were identified, namely, a W/Z-associated coding gene (cytochrome P450 4c3-like, MrCYP4), and a Z-specific long noncoding RNA (MrZlnc). Silencing of these two candidates caused a reduction in MrIAG expression and vice versa, clearly indicating crosstalk with the IAG-switch. The study thus constitutes the first step in unravelling key elements that control sexual differentiation in crustaceans exhibiting WZ-ZZ sex inheritance.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144483103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"In Vitro Maturation of Bovine and Porcine Oocytes as a Versatile Model for Toxicity and Metabolism Studies†.","authors":"Ida Hallberg, Denise Laskowski, Ylva C B Sjunnesson","doi":"10.1093/biolre/ioaf137","DOIUrl":"https://doi.org/10.1093/biolre/ioaf137","url":null,"abstract":"<p><p>Assisted reproductive technologies (ARTs) have significantly advanced, yet the definition and assessment of oocyte quality remain pivotal for successful embryo development. Oocyte quality is influenced by various factors, including lifestyle, age, metabolic status, and exposure to pollutants or medications. Non-invasive techniques to evaluate oocyte quality are limited, and invasive methods such as morphological assessments by fluorescent staining and genetic analyses are commonly used in research. In vitro maturation (IVM) of oocytes from cows and pigs offers valuable models for human ART due to various similarities, though there are species-specific differences. These IVM and in vitro embryo production (IVEP) systems offer valuable insights into oocyte maturation and early embryo development without use of experimental animals. While bovine IVEP is well-established and commonly used in research, porcine IVEP have great potential for improvement. The models can be crucial for reproductive biology and biomedical research and can contribute to advancements in areas such as human fertility treatments and toxicity testing. Apart from oocyte quality assessment, there are challenges, such as the variability in oocyte quality from slaughterhouse ovaries and the difficulty in mimicking the complex in vivo environment. Emerging technologies, like gene-expression analysis tools, and non-invasive -omics approaches hold promise for overcoming some obstacles by improving precision, sensitivity, and reproducibility in IVEP studies. In this review, we discuss the model in simulating various conditions but with main focus on metabolic challenges and in toxicity screening, and aim to give an overview of the current and possible future use of IVM in cows and pigs as a model for the same or other mammalian species, as well as the limitations.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delayed gonadal development of estrogen-induced ZZ females in Chinese soft-shelled turtles†.","authors":"An Huang, Jiequn Tang, Jinni Wang, Qiran Chen, Wei Sun, Ling Xiao, Guoying Qian, Yin Guo, Chutian Ge","doi":"10.1093/biolre/ioaf131","DOIUrl":"https://doi.org/10.1093/biolre/ioaf131","url":null,"abstract":"<p><p>Exogenous estrogen induces sex reversal during embryonic development in various animal species including reptiles, yet the mechanisms underlying post-reversal gonadal development remain poorly understood. In this study, we generated ZZ female Chinese soft-shelled turtle Pelodiscus sinensis through 17β-estradiol treatment and investigated their reproductive traits and sex-specific gene expression at 1, 3, 6, 8 and 12 months post-hatching. Our results showed that by 12 months, ZZ females maintained ovarian structures and exhibited low expression levels of the male-specific genes SOX9 and DMRT1. However, at 8 months, the gonadosomatic index of ZZ female becomes significantly lower than that of ZW females, and yellow follicles were absent. Furthermore, the expression of female granulosa and theca cell markers FOXL2 and CYP19A1 in ZZ females was significantly lower than in ZW females. Our findings indicate that while sex-reversed ZZ female turtles retain female gonadal phenotypes, they experience delayed ovarian development at 8 months. The findings directly inform all-male aquaculture strategies by clarifying estrogen's long-term effects on gonadal development.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Restricted Mating Windows and Thrombospondin 2 Gene Deletion Improve the Precision of Parturition Timing in Mice.","authors":"Jackson H Rogers, Carmel Martin-Fairey, Naoko Boatwright, Alexus J Brown, Edie Charron, Shajila Siricilla, Ronald McCarthy, Sarah K England, Bibhash C Paria, Anita Mahadevan-Jansen, Jeff Reese, Jennifer L Herington","doi":"10.1093/biolre/ioaf132","DOIUrl":"https://doi.org/10.1093/biolre/ioaf132","url":null,"abstract":"<p><p>Mice are an important model organism for studying reproduction, parturition timing, and embryonic development. Previous reports indicate that limiting mouse breeding periods can reduce variability in fetal weight. To expand on these findings, this study sought to determine whether a restricted breeding window affects mating receptivity, precision of parturition timing, and pregnancy outcomes compared to standard overnight breeding of two common wild-type strains (C57BL/6 J and CD-1) of laboratory mice. Since Thrombospondin-2 (Thbs2) knockout (KO) mice exhibit premature cervical remodeling without a shortened gestational length, we examined whether these mutant mice experience more precise parturition timing compared to their wild-type (WT) controls. Our findings revealed that the percentage of CD-1, but not C57BL/6 J, female mice that were copulatory plug positive was significantly (p < 0.0001) reduced following a 2-hour restricted mating period in the morning or evening compared to overnight breeding. Conversely, using a 2-hour restricted breeding period in the morning or evening significantly (p < 0.0001) reduced the pregnancy rate of C57BL/6 J, but not CD-1, female mice. Additionally, the variance in the gestational length and the timing of parturition was significantly (p < 0.0001) less in C57BL/6 J and CD-1 females bred during a 2-hour period in the morning compared to either strain bred overnight. Interestingly, Thbs2 KO mice mated overnight had the shortest variance in parturition times. Pregnancy outcomes were not significantly impacted by breeding schedules or Thbs2 genotype. Collectively, these data reveal that restricted mating windows and deletion of the Thbs2 gene lead to more precise timing of parturition.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The downregulation of FKBP5 contributes to the pathogenesis of adenomyosis and is associated with impaired endometrial receptivity†.","authors":"Meng Wang, Yungai Xiang, Dan Zhang, Xujing Geng, Xiting Han, Peipei Guo, Le Zhang, Li Tan","doi":"10.1093/biolre/ioaf126","DOIUrl":"https://doi.org/10.1093/biolre/ioaf126","url":null,"abstract":"<p><p>Adenomyosis is characterized by the infiltration of endometrial glands and stroma into the myometrium which affects about 35% women at reproductive age. Women affected by adenomyosis always experience issues such as defective decidualization, reduced endometrial receptivity, disrupted embryo-maternal communication, and challenges with implantation. However, the underlying mechanism remains obscure. We analyzed three gene expression profiling datasets (GSE244236, GSE190580 and GSE157718) and identified that FKBP5 was significantly reduced in endometrium from patients with adenomyosis. Primary endometrial stromal cells (ESCs) from patients had increased proliferation, reduced apoptosis and elevated migration capacity. Knockdown FKBP5 in ESCs upregulated proliferation, repressed apoptosis, and impaired the secretion of prolactin and IGFBP-1 during in vitro decidualization. Meanwhile, repressing FKBP5 in ESCs promoted oxygen consumption rate, extracellular acidification rate, and ATP production. Mechanically, knockdown FKBP5 in ESCs activated AKT signaling by promoting its phosphorylation at Ser-473. FKBP5 was downregulated in patients with adenomyosis which contributed to impaired ESCs function and the process of decidualization providing a candidate target for adenomyosis treatment.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental programming: mechanisms of early exposure to real-life chemicals in biosolids on offspring ovarian dynamics†.","authors":"Yiran Zhou, Katherine M Halloran, Michelle Bellingham, Richard G Lea, Neil P Evans, Kevin D Sinclair, Peter Smith, Vasantha Padmanabhan","doi":"10.1093/biolre/ioaf053","DOIUrl":"10.1093/biolre/ioaf053","url":null,"abstract":"<p><p>Female reproductive capacity is shaped by ovarian reserve and patterns of follicle development. Ovarian reserve depletion occurs by follicle activation and atresia, which are affected by environmental chemicals (ECs). Because humans are simultaneously exposed to hundreds of ECs, real-life exposure models are essential to assess patterns of atresia after EC exposure. Previous findings demonstrate maternal preconceptional and gestational EC exposure via biosolids increases activation rate and reduces primordial follicle pool in juvenile, but not adult sheep. We hypothesized that this shift involves changes in death and proliferative pathways that impact follicle atresia from juvenile to adult life. Ovaries were collected from juvenile (9.5 weeks) and adult (2.5 years) offspring from ewes grazed on biosolids-treated pasture (BTP) or inorganic fertilizer-treated pasture (Control). Follicular atresia was assessed through morphological characteristics and molecular death pathways, including expression of markers for apoptosis (CASP3), autophagy (LC3), ferroptosis (GPX4), and proliferation (Ki67). There were higher levels of apoptosis and autophagy, and lower proliferation, in juvenile BTP offspring compared to controls. In adult BTP offspring, apoptosis and proliferation were similar, autophagy was lower, and ferroptosis was higher compared to controls. Apoptosis was lower and ferroptosis was higher in adults than juveniles, regardless of treatment. Adult BTP offspring had lower autophagy and similar proliferation levels than juvenile BTP offspring. These findings suggest that lower autophagy and lack of decrease in proliferation contribute to normalization of activation rate and ovarian pool in BTP adult offspring and supportive of lasting impacts of gestational EC exposure on offspring follicular health.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1229-1242"},"PeriodicalIF":3.1,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12191645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interferon tau-dependent and -independent changes in the bovine corpus luteum of early pregnancy†.","authors":"Camilla H K Hughes, Adelaide C Hellmers, M Isabel da Silva, Troy L Ott, Joy L Pate","doi":"10.1093/biolre/ioaf044","DOIUrl":"10.1093/biolre/ioaf044","url":null,"abstract":"<p><p>The effect of interferon tau (IFNT) on the uterus is critical for maternal recognition of pregnancy in ruminants, while its direct role in luteal function is less well understood. To address this, we performed two experiments. In Experiment 1, cattle received intrauterine infusions of either: bovine serum albumin (BSA; vehicle) or vehicle with IFNT from Days 14 to 16 of the estrous cycle or vehicle with IFNT from Days 14 to 19 or vehicle with IFNT from Days 14 to 19 with pregnancy associated glycoprotein (PAG) from Days 17 to 19. Corpora lutea (CL) were collected on Day 17 or 20 and RNAseq was performed. In Experiment 2, cultured luteal steroidogenic cells from cyclic (Days 10-12) cattle were treated with IFNT and RNAseq was performed. Treatment with IFNT resulted in luteal changes (in vivo: 130 transcripts; in vitro: 2981 transcripts), while addition of PAG resulted in 13 changed transcripts. Only 31% of the genes that changed in the CL during early pregnancy (Hughes et al., 2020) were regulated by IFNT; these were antiviral and immune regulators. In contrast, 50% of the genes that changed during early pregnancy were not regulated by IFNT and were associated with cellular proliferation and extracellular matrix organization. The remaining 19% of genes were not conclusively identified as either IFNT regulated or non-regulated. This suggests that the temporal changes in the CL during early pregnancy are only partially regulated by IFNT, drawing into question identities of other luteal regulators or the effect of age of CL.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1213-1228"},"PeriodicalIF":3.1,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure of bovine granulosa cells to lipopolysaccharide reduces progesterone secretion during luteinization†.","authors":"Zachary K Seekford, Stephanie E Wohlgemuth, I Martin Sheldon, John J Bromfield","doi":"10.1093/biolre/ioaf055","DOIUrl":"10.1093/biolre/ioaf055","url":null,"abstract":"<p><p>Uterine disease reduces fertility in dairy cows and is caused by pathogenic bacteria. During disease, lipopolysaccharide accumulates in follicular fluid and triggers granulosa cell inflammation via the Toll-like receptor 4 pathway. Follicle growth and plasma estradiol are reduced in cows with uterine disease, and treatment of bovine granulosa cells with lipopolysaccharide reduces cytochrome P450 family 19 subfamily A member 1 (CYP19A1) expression and estradiol synthesis. It is unclear whether the effects of lipopolysaccharide on the steroidogenic capacity of granulosa cells persist in cells during luteinization. We hypothesized that acute exposure of granulosa cells to lipopolysaccharide would alter progesterone synthesis during luteinization. Here, we demonstrate that acute exposure of granulosa cells to lipopolysaccharide reduces progesterone synthesis during a 9-day period of luteinization after lipopolysaccharide treatment. We show that exposure of granulosa cells to lipopolysaccharide does not alter the gene expression of steroidogenic acute regulatory protein (STAR), hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (HSD3B1), or cytochrome P450 family 11 subfamily A member 1 (CYP11A1), or cellular respiration during luteinization. However, acute exposure of granulosa cells to lipopolysaccharide reduces the abundance of intracellular lipid, mitochondria density, and cholesterol uptake during luteinization, suggesting a potential mechanism of altered steroidogenesis after acute inflammation. Collectively, these findings show that exposure of granulosa cells to lipopolysaccharide reduces progesterone synthesis during luteinization, which is associated with altered lipid droplets and mitochondria accumulation required for steroidogenesis. Perturbations to granulosa cell physiology during uterine disease may have prolonged effects on ovarian function that contribute to reduced fertility of cows. Understanding the effects of uterine disease on corpus luteum function after disease resolution can help explain disease associated subfertility in cattle.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1243-1255"},"PeriodicalIF":3.1,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}