Biology of Reproduction最新文献_第5页

Tendency towards clonality: deviations of meiosis in parthenogenetic Caucasian rock lizards. 克隆倾向：孤雌繁殖的高加索岩蜥蜴减数分裂的偏差。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-04-17 DOI: 10.1093/biolre/ioaf091

Victor Spangenberg, Marine Arakelyan, Sergey A Simanovsky, Yana Dombrovskaya, Emma R Khachatrian, Oxana Kolomiets

{"title":"Tendency towards clonality: deviations of meiosis in parthenogenetic Caucasian rock lizards.","authors":"Victor Spangenberg, Marine Arakelyan, Sergey A Simanovsky, Yana Dombrovskaya, Emma R Khachatrian, Oxana Kolomiets","doi":"10.1093/biolre/ioaf091","DOIUrl":"https://doi.org/10.1093/biolre/ioaf091","url":null,"abstract":"Cytogenetic mechanisms of unisexuality in diploid parthenogenetic species of the genus Darevskia have remained debatable until recently. The mechanism that allows the unisexual form to maintain heterozygosity in a number of generations is important for its long-term existence in nature. In this work, for the first time, for parthenogenetic species of the genus Darevskia, in addition to primary oocytes with the usual ploidy (18+ZW bivalents in meiotic prophase I), oocytes that underwent premeiotic genome endoduplication and carried a doubled number of bivalents (36+ZZ+WW) were found. Here, we present a detailed comparative analysis of the preparations of synaptonemal complexes in oocyte nuclei without and with genome endoduplication and the behavior of sex Z and W chromosomes. We show the details of the assembly of bivalents in pachytene nuclei, where either homeologs or doubled identical copies of chromosomes compete for synapsis and form multivalents. For the first time, the WW sex bivalent has been visualized in parthenogenetic reptiles. We show the reverse side of meiotic deviations in obligate parthenogenesis - cases of nonviable embryos with specific abnormalities.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved Evaluation of Mitochondrial Membrane Potential in Bovine Spermatozoa Using JC-1 with Flow Cytometry. 流式细胞术改进JC-1对牛精子线粒体膜电位的评价。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-04-14 DOI: 10.1093/biolre/ioaf081

Abigail L Zezeski, Lauren E Hamilton, Thomas W Geary

{"title":"Improved Evaluation of Mitochondrial Membrane Potential in Bovine Spermatozoa Using JC-1 with Flow Cytometry.","authors":"Abigail L Zezeski, Lauren E Hamilton, Thomas W Geary","doi":"10.1093/biolre/ioaf081","DOIUrl":"https://doi.org/10.1093/biolre/ioaf081","url":null,"abstract":"One of the most popular methods for measuring mitochondrial membrane potential via conventional flow cytometry in spermatozoa is through the use of the fluorescent dye 5,5,6,6'-tetrachloro-1,1',3,3'tetraethylbenzimi-dazoylcarbocyanine iodide (JC-1). The JC-1 dye is unique in that it will fluoresce green (525 nm, monomers) or red-orange (595 nm, J-aggregates) when excited to indicate depolarized or polarized mitochondria. While JC-1 can be multiplexed with viability dyes such as propidium iodide and SYBR 14 for microscopy, doing so for conventional flow cytometry has proved difficult due to overlap of the emission spectra between dyes. The objective of this protocol is to improve the accuracy of JC1 J-aggregate quantification in conventional flow cytometry by using a 405 nm or 532 nm laser for excitation coupled with the viability dye calcein violet (CV). Quantification of live J-aggregates when excited with a 405 nm or 532 nm laser is more strongly correlated to progressive motility (405 nm: r = 0.73, P < 0.0001; 532 nm: r = 0.52, P = 0.0002) and viability (405 nm: r = 0.93, P < 0.0001; 532 nm: r = 0.74, P < 0.0001) than quantification with the traditional 488 nm laser excitation and dual emission of 525 nm and 595 nm (progressive motility: r = 0.05, P = 0.72; viability: r = 0.21, P = 0.14). The use of a 405 nm or a 532 nm laser requires no compensation. This allows for clear identification of the polarized J-aggregates, and when combined with CV, may help identify which spermatozoa have the greatest fertilization potential.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in endometriosis research: animal models for the study of reproductive disorders. 子宫内膜异位症的研究进展：研究生殖障碍的动物模型。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-04-14 DOI: 10.1093/biolre/ioaf089

Mingjuan Zhou, Xingchen Zhou, Xipeng Wang

引用次数: 0

Rictor stability mediated by USP9X regulates embryo implantation by participating in lipid metabolism of endometrium. USP9X介导的Rictor稳定性通过参与子宫内膜脂质代谢调节胚胎着床。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-04-14 DOI: 10.1093/biolre/ioaf088

Mingyu Peng, Junlin He, Xueqing Liu, Xinyi Mu, Xin Yin, Taihang Liu, Xuemei Chen, Rufei Gao, Yingxiong Wang, Qian Feng, Yanqing Geng

{"title":"Rictor stability mediated by USP9X regulates embryo implantation by participating in lipid metabolism of endometrium.","authors":"Mingyu Peng, Junlin He, Xueqing Liu, Xinyi Mu, Xin Yin, Taihang Liu, Xuemei Chen, Rufei Gao, Yingxiong Wang, Qian Feng, Yanqing Geng","doi":"10.1093/biolre/ioaf088","DOIUrl":"https://doi.org/10.1093/biolre/ioaf088","url":null,"abstract":"The receptive endometrium is a prerequisite for successful embryo implantation, and abnormal endometrial receptivity would lead to infertility. Many key proteins involved in endometrial receptivity have been confirmed to undergo post transcriptional modifications. However, there are limited reports on deubiquitination modification during this process. Our previous studies found that Rictor participated in the endometrial receptivity, and maintained at a high level in the endometrium during implantation, but the mechanism for maintaining stability of Rictor protein remains unclear. Here, we showed that USP9X expression in endometrium was dynamic with the establishment of endometrial receptivity, and promoted the protein stability of Rictor through deubiquitination. Inhibition of USP9X could suppress the adhesion action of trophoblast cells to endometrial epithelial cells, reduce the filamentous pseudopodia of epithelial cells, and inhibit the epithelial mesenchymal transformation. Rictor is partially responsible for the derailment of epithelial cell transformation in response to USP9X inhibition. Membrane fluidity mediated by lipid metabolism is involved in regulation of Rictor on endometrial receptivity. This study revealed the role of USP9X in endometrial receptivity for the first time, and confirmed that Rictor was the target protein of USP9X in endometrium. In addition, we described the unique lipidomics characteristics of the endometrial epithelial cells regulated by Rictor. These data would further improve the molecular network of endometrial receptivity, supplement the regulatory factors of lipid metabolism in endometrial cells, and provide insights into the new therapeutics, pre-diagnosis and preventive strategies for the derailment of endometrial receptivity and subsequently adverse \"ripple effect\" including infertility.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

METTL7A improves bovine IVF embryo competence by attenuating oxidative stress†. METTL7A通过减轻氧化应激提高牛体外受精胚胎能力。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-04-13 DOI: 10.1093/biolre/ioaf018

Linkai Zhu, Hao Ming, Giovanna N Scatolin, Andrew Xiao, Zongliang Jiang

{"title":"METTL7A improves bovine IVF embryo competence by attenuating oxidative stress†.","authors":"Linkai Zhu, Hao Ming, Giovanna N Scatolin, Andrew Xiao, Zongliang Jiang","doi":"10.1093/biolre/ioaf018","DOIUrl":"10.1093/biolre/ioaf018","url":null,"abstract":"In vitro fertilization is a widely used assisted reproductive technology to achieve a successful pregnancy. However, the acquisition of oxidative stress in embryo in vitro culture impairs its competence. Here, we demonstrated that a nuclear coding gene, methyltransferase-like protein 7A, improves the developmental potential of bovine embryos. We found that exogenous methyltransferase-like protein 7A modulates expression of genes involved in embryonic cell mitochondrial pathways and promotes trophectoderm development. Surprisingly, we discovered that methyltransferase-like protein 7A alleviates mitochondrial stress and DNA damage and promotes cell cycle progression during embryo cleavage. In summary, we have identified a novel mitochondria stress eliminating mechanism regulated by methyltransferase-like protein 7A that occurs during the acquisition of oxidative stress in embryo in vitro culture. This discovery lays the groundwork for the development of methyltransferase-like protein 7A as a promising therapeutic target for in vitro fertilization embryo competence.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"628-639"},"PeriodicalIF":3.1,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11996759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the role of FOXL2 in female differentiation and disease: a comprehensive review†. 揭示FOXL2在女性分化和疾病中的作用：综述

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-04-13 DOI: 10.1093/biolre/ioaf013

Jia He, Zican Wang, Lici Yang, Yongjian Jiang, Ge Yan, Yongwei Pan, Fei Gao, Jinxiang Yuan, Yang Gao

{"title":"Unveiling the role of FOXL2 in female differentiation and disease: a comprehensive review†.","authors":"Jia He, Zican Wang, Lici Yang, Yongjian Jiang, Ge Yan, Yongwei Pan, Fei Gao, Jinxiang Yuan, Yang Gao","doi":"10.1093/biolre/ioaf013","DOIUrl":"10.1093/biolre/ioaf013","url":null,"abstract":"Ovarian differentiation relies on the accurate and orderly expression of numerous related genes. Forkhead box protein L2 (FOXL2) is one of the earliest ovarian differentiation markers and transcription factors. In sex determination, FOXL2 maintains the differentiation of the female pathway by inhibiting male differentiation genes, including SOX9 and SF1. In addition, FOXL2 promotes the synthesis of follicle-stimulating hormone and anti-Müllerian hormone to support follicle development. Mutations in FOXL2 are associated with numerous female reproductive diseases. A comprehensive and in-depth study of FOXL2 provides novel strategies for the diagnosis and treatment of such diseases. This review discusses the mechanism of FOXL2 in female sex differentiation and maintenance, hormone synthesis, and disease occurrence and reveals the role of FOXL2 as a central factor in female sex development and fertility maintenance. This review will serve as a reference for identifying novel targets of other regulatory factors interacting with FOXL2 in female sex determination and follicle development and for the diagnosis and treatment of female reproductive diseases.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"600-613"},"PeriodicalIF":3.1,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: The pathogenesis of endometriosis and adenomyosis: insights from single-cell RNA sequencing. 子宫内膜异位症和子宫腺肌症的发病机制：来自单细胞RNA测序的见解。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-04-13 DOI: 10.1093/biolre/ioae184

引用次数: 0

Correction to: Roles of histone post-translational modifications in meiosis. 更正：组蛋白翻译后修饰在减数分裂中的作用。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-04-13 DOI: 10.1093/biolre/ioaf024

引用次数: 0

SHP2 regulates the HIF-1 signaling pathway in the decidual human endometrial stromal cells†. SHP2在人子宫内膜间质细胞中调控HIF-1信号通路。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-04-13 DOI: 10.1093/biolre/ioaf019

Liqun Ouyang, Xia Gao, Rongyu Yang, Peiyi Zhou, Han Cai, Yingpu Tian, Haibin Wang, Shuangbo Kong, Zhongxian Lu

{"title":"SHP2 regulates the HIF-1 signaling pathway in the decidual human endometrial stromal cells†.","authors":"Liqun Ouyang, Xia Gao, Rongyu Yang, Peiyi Zhou, Han Cai, Yingpu Tian, Haibin Wang, Shuangbo Kong, Zhongxian Lu","doi":"10.1093/biolre/ioaf019","DOIUrl":"10.1093/biolre/ioaf019","url":null,"abstract":"The decidual endometrial stromal cells play a critical role in the establishment of uterine receptivity and pregnancy in human. Our previous studies demonstrate that protein tyrosine phosphatase 2 SHP2 is highly expressed in decidualized cells and governs the decidualization progress. However, the role and mechanism of SHP2 in the function of decidual cells remain unclear. Here, we screened proteins interacting with SHP2 in decidual hTERT-immortalized human endometrial stromal cells (T-HESCs) and identified Hypoxia-inducible factor-1 (HIF-1) signaling pathway as a potential SHP2-mediated signaling pathway through proximity-dependent biotinylation (BioID) analysis. Immunoprecipitation (Co-IP) revealed an interaction between SHP2 and HIF-1α, which colocalized to the nucleus in decidual cells. Furthermore, the SHP2 expression correlated with the transcriptional activation of HIF-1α and its downstream genes Beta-enolase (Eno3), Pyruvate kinase 2 (Pkm2), Aldolase C (Aldoc), and Facilitative glucose transporter 1 (Glut1). Knockdown or inhibition of SHP2 significantly reduced the mRNA and protein levels of HIF-1α and its downstream genes, as well as lactate production in decidual cells. We also established a hypoxia model of T-HESCs and 293 T cells and found that hypoxic treatment induced the expression of SHP2 and HIF-1α, which colocalized in the nucleus. SHP2 forced-expression rescued the inhibitory effects of SHP2 deficiency on HIF-1α expression and lactate production. Finally, SHP2 binds to the promoter regions of HIF-1α and its target genes (Eno3, Pkm2, Aldoc, and Glut1). Collectively, our results suggest that SHP2 influences the function of decidual cells by HIF-1α signaling and provide a novel function mechanism of decidual stromal cells.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"743-753"},"PeriodicalIF":3.1,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphatase and tensin homolog deficiency induces M2 macrophage polarization by promoting glycolytic activity in endometrial stromal cells. 磷酸酶和紧张素同源性缺乏通过促进子宫内膜基质细胞糖酵解活性诱导M2巨噬细胞极化。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-04-13 DOI: 10.1093/biolre/ioaf016

Fengqin Dai, Jinjin Li, Yingwei Liu

{"title":"Phosphatase and tensin homolog deficiency induces M2 macrophage polarization by promoting glycolytic activity in endometrial stromal cells.","authors":"Fengqin Dai, Jinjin Li, Yingwei Liu","doi":"10.1093/biolre/ioaf016","DOIUrl":"10.1093/biolre/ioaf016","url":null,"abstract":"Endometriosis is a common gynecological disorder, whose pathogenesis remains incompletely understood. Macrophages, a key type of immune cell, are pivotal in the context of endometriosis. This study seeks to explore the interactions between endometriotic cells and macrophages. Quantitative real-time PCR (qRT-PCR) and Western blot experiments were employed to detect phosphatase and tensin homolog (PTEN) expression. Glucose consumption, lactate production, extracellular acidification rate, and oxygen consumption rate levels were used to assess cellular glycolytic capacity. The interaction between conditioned media from ectopic endometrial stromal cells (EESCs) and macrophages was investigated through co-culture experiments. The expression of M2 macrophage marker proteins and inflammatory factors was detected via qRT-PCR, immunofluorescence staining, and enzyme-linked immunosorbent assay. Cellular functions were evaluated using Cell Counting Kit-8, 5-Ethynyl-2'-deoxyuridine (EdU), and wound healing assays. We found that PTEN deficiency promoted the glycolytic activity of EESCs. Simultaneously, it significantly promoted the macrophages' polarization toward the M2 phenotype, demonstrated by increased expression of M2 markers (differentiation 206 (CD206), CD163, and (C-C motif) ligand 22 (CCL22)). Further studies revealed that PTEN-deficient EESCs increased the level of CCL2 via promoting glycolytic activity, which was reversed by glycolytic inhibitor. Moreover, lactate and conditioned media from overexpressed CCL2 EESCs facilitated M2 polarization of macrophages, while 2-deoxy-d-glucose reversed the promoting effect. Furthermore, lactate-facilitated macrophages promoted the proliferation and migration abilities of EESCs. PTEN deficiency induces M2 macrophage polarization by promoting glycolytic activity in EESCs, which deepens the knowledge of the pathophysiology of endometriosis and provides novel insights into its treatment.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"640-650"},"PeriodicalIF":3.1,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0