Biology of Reproduction最新文献

The RNA-binding protein road map of spermatogenesis. 精子发生的 RNA 结合蛋白路线图

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-24 DOI: 10.1093/biolre/ioae138

P Jeremy Wang

引用次数: 0

Replacing sperm with genotyped haploid androgenetic blastomeres to generate cattle with predetermined paternal genomes. 用基因分型的单倍体雄性胚泡替代精子，生成具有预定父系基因组的牛。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-20 DOI: 10.1093/biolre/ioae135

Luis Aguila, Rafael V Sampaio, Jacinthe Therrien, Ricardo P Nociti, Remi Labrecque, Amelie Tremblay, Gabrielle Marras, Patrick Blondin, Lawrence C Smith

{"title":"Replacing sperm with genotyped haploid androgenetic blastomeres to generate cattle with predetermined paternal genomes.","authors":"Luis Aguila, Rafael V Sampaio, Jacinthe Therrien, Ricardo P Nociti, Remi Labrecque, Amelie Tremblay, Gabrielle Marras, Patrick Blondin, Lawrence C Smith","doi":"10.1093/biolre/ioae135","DOIUrl":"https://doi.org/10.1093/biolre/ioae135","url":null,"abstract":"Although meiosis plays an essential role for the survival of species in natural selection, the genetic diversity resulting from sexual reproduction impedes human-driven strategies to transmit the most suitable genomes for genetic improvement, forcing breeders to select diploid genomes generated after fertilization, that is, after the encounter of sperm and oocytes carrying unknown genomes. To determine whether genomic assessment could be used before fertilization, some androgenetic haploid morula-stage bovine embryos derived from individual sperm were biopsied for genomic evaluation and others used to reconstruct \"semi-cloned\" (SC) diploid zygotes by the intracytoplasmic injection into parthenogenetically activated oocytes, and the resulting embryos were transferred to surrogate females to obtain gestations. Compared to controls, in vitro development to the blastocyst stage was lower and fewer surrogates became pregnant from the transfer of SC embryos. However, fetometric measurements of organs and placental membranes of all SC conceptuses were similar to controls, suggesting a normal post-implantation development. Moreover, transcript amounts of imprinted genes IGF2, IGF2R, PHLDA2, SNRPN and KCNQ1OT1 and methylation pattern of the KCNQ1 DMR were unaltered in SC conceptuses. Overall, this study shows that sperm can be replaced by genotyped haploid embryonic-derived cells to produce bovine embryos carrying a predetermined paternal genome and viable first trimester fetuses after transfer to female recipients.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142280109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The loss of dNK1/2 and EVT1 cells at the maternal-fetal Interface is associated with recurrent miscarriage. 母胎界面 dNK1/2 和 EVT1 细胞的丢失与反复流产有关。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-20 DOI: 10.1093/biolre/ioae136

Yijun Yang, Jiangnan Qiu, Qiaoqiao Xu, Yun Fan, Hui Wang, Hong Qian, Zhu Wu, Yuchen Zhang, Yingchun Gao, Can Shi, Chuncheng Lu, Yankai Xia, Wenjun Cheng

{"title":"The loss of dNK1/2 and EVT1 cells at the maternal-fetal Interface is associated with recurrent miscarriage.","authors":"Yijun Yang, Jiangnan Qiu, Qiaoqiao Xu, Yun Fan, Hui Wang, Hong Qian, Zhu Wu, Yuchen Zhang, Yingchun Gao, Can Shi, Chuncheng Lu, Yankai Xia, Wenjun Cheng","doi":"10.1093/biolre/ioae136","DOIUrl":"https://doi.org/10.1093/biolre/ioae136","url":null,"abstract":"Recurrent miscarriage (RM) is a chronic and heterogeneous pregnancy disorder lacking effective treatment. Alterations at the maternal-fetal interface are commonly observed in RM, with the loss of certain cell subpopulations believed to be a key cause. Through single-cell sequencing of RM patients and healthy donors, we aim to identify aberrancy of cellular features in RM tissues, providing new insights into the research. Natural killer (NK) cells, the most abundant immune cells in the decidua, are traditionally classified into dNK1, dNK2, and dNK3. In this study, we identified a new subset, dNK1/2, absent in RM tissues. This subset was named because it expresses biomarkers of both dNK1 and dNK2. With further analysis, we discovered that dNK1/2 cells play roles in immunoregulation and cytokine secretion. On the villous side of the interface, a notable decrease of extravillous trophoblast (EVT) cells was identified in RM tissues. We clustered EVTs into EVT1 (absent in RM) and EVT2 (retained in RM). Pseudotime analysis revealed distinct differentiation paths, identifying CCNB1, HMGB1, and NPM1 as EVT1 biomarkers. Additionally, we found that EVT1 is involved in the regulation of cell death, while EVT2 exhibited more angiogenic activity. Cell communication analysis revealed that interaction between EVT1 and dNK1/2 mediates chemotaxis and endothelial cell regulation, crucial for spiral artery remodeling. The loss of this interaction may impair decidualization, which is associated with RM. In summary, we propose that the loss of dNK1/2 and EVT1 cells is a significant pathological feature of RM.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142280110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of CCDC188 causes male infertility with defects in the sperm head–neck connection in mice CCDC188 缺失会导致小鼠精子头颈部连接缺陷，从而导致雄性不育

IF 3.6 2区生物学

Biology of Reproduction Pub Date : 2024-09-18 DOI: 10.1093/biolre/ioae137

Yumiao Qiu, Keisuke Shimada, Kaito Yamamoto, Masahito Ikawa

{"title":"Loss of CCDC188 causes male infertility with defects in the sperm head–neck connection in mice","authors":"Yumiao Qiu, Keisuke Shimada, Kaito Yamamoto, Masahito Ikawa","doi":"10.1093/biolre/ioae137","DOIUrl":"https://doi.org/10.1093/biolre/ioae137","url":null,"abstract":"Acephalic spermatozoa syndrome (ASS) represents a rare genetic and reproductive disease, which is defined as semen composed of mostly headless spermatozoa. The connecting piece in the neck region, also known as the head-to-tail coupling apparatus (HTCA), plays a crucial role in the tight linkage between the sperm head and tail. Dysfunction of this structure can lead to separation of sperm heads and tails, and male infertility. Using the mouse as an experimental model, several proteins have been identified as associated with the HTCA and disruption of these proteins causes acephalic spermatozoa. However, the molecular mechanism underlying this morphologic anomaly and HTCA remains elusive. In this study, we focused on coiled-coil domain containing 188 (Ccdc188), which shows testis-enriched expression. To elucidate the physiological role of CCDC188, we generated a knockout (KO) mouse line using the CRISPR/Cas9 system. Ccdc188 KO male mice were sterile, indicating that CCDC188 is indispensable for male fertility. Most Ccdc188-null spermatozoa were acephalic. Transmission electron microscopy revealed that while the sperm HTCA could assemble properly without CCDC188, the HTCA failed to attach to the nucleus during spermiogenesis, leading to sperm head and neck separation. In addition, we found almost all of the spermatozoa in the cauda epididymis lacked a mitochondrial sheath. Taken together, we demonstrated that CCDC188 plays a crucial role in forming a tight sperm head–neck junction.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding recurrent pregnancy loss: insights from comparative proteomics studies 解码复发性妊娠损失：比较蛋白质组学研究的启示

IF 3.6 2区生物学

Biology of Reproduction Pub Date : 2024-09-17 DOI: 10.1093/biolre/ioae134

Katarina Davalieva, Damjan Kocarev, Dijana Plaseska-Karanfilska

{"title":"Decoding recurrent pregnancy loss: insights from comparative proteomics studies","authors":"Katarina Davalieva, Damjan Kocarev, Dijana Plaseska-Karanfilska","doi":"10.1093/biolre/ioae134","DOIUrl":"https://doi.org/10.1093/biolre/ioae134","url":null,"abstract":"Recurrent pregnancy loss (RPL) represents a common disorder that affects up to 2% of the women aiming at childbirth with long-term consequences on family and society. Factors contributing to it in more than half of the cases are still unknown. Comparative proteomic analysis can provide new insights into the biological pathways underlining the pathogenesis of RPL. Until now, chorionic villi, decidua, placenta, endometrium, and maternal blood from women with RPL have been analyzed by comparative proteomics studies. In this review, we aimed to provide a critical evaluation of the published comparative studies of RPL on human samples, gathered by systematic literature search using PubMed and Google Scholar databases. We provide a detailed overview of the analyzed materials, proteomics platforms, proposed candidate biomarkers and altered pathways and processes linked with RPL. The top, most identified and validated biomarker candidates from all studies are discussed, followed by bioinformatics analysis of the available high-throughput data and presentation of common altered processes and pathways in RPL. Finally, future directions aimed at developing new and efficient therapeutic strategies are discussed as well.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro reminiscence: uterine programming in vivo affects respective luminal epithelial cells function in vitro†. 体外回忆：子宫体内编程影响各自管腔上皮细胞的体外功能†。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-14 DOI: 10.1093/biolre/ioae093

Cecilia Constantino Rocha, Andrey Luiz Lopes Cordeiro, Meghan Campbell, Mariangela Bueno Cordeiro Maldonado, Felipe Alves Correa Carvalho Silva, Alexandra Bennett, Abdul Waheed, Thomas Hansen, Mario Binelli

{"title":"In vitro reminiscence: uterine programming in vivo affects respective luminal epithelial cells function in vitro†.","authors":"Cecilia Constantino Rocha, Andrey Luiz Lopes Cordeiro, Meghan Campbell, Mariangela Bueno Cordeiro Maldonado, Felipe Alves Correa Carvalho Silva, Alexandra Bennett, Abdul Waheed, Thomas Hansen, Mario Binelli","doi":"10.1093/biolre/ioae093","DOIUrl":"10.1093/biolre/ioae093","url":null,"abstract":"In cattle, the endometrium during diestrus and early pregnancy displays cellular responses that are consequences of prior, transient stimuli. Goal was to establish a model to study cellular memory in the endometrium. The hypothesis is that stimuli given to endometrium in vivo are retained as a cellular memory that remains after bovine uterine epithelial cells (BUECs) are isolated, cultured, and further stimulated in vitro. Objectives were to measure BUEC proliferation/migration and responsiveness to recombinant bovine Interferon-tau (rbIFNT) in vitro: among cows that showed estrus (experiment 1 [Exp1]), cows that became or not pregnant to artificial insemination (Exp2), cows that received or not supplemental progesterone (P4; Exp3) and cows that received or not a COX-1/2 inhibitor (Exp4). Only cows that displayed estrus were included in studies. For all experiments endometrial cytology was collected 4 days after estrus, BUECs were cultured, propagated, and submitted to rbIFNT treatment and an in vitro scratch assay. In Exp1, different cows spontaneously grouped according to proliferative/migratory capacity and responsiveness to rbIFNT of their respective BUECs. In Exp2, BUECs from pregnant cows showed greater rbIFNT responsiveness and cellular proliferation. In Exp3, BUECs from cows supplemented with P4 presented inhibited proliferation and increased expression of RSAD2. In Exp4, Flunixin Meglumine modified rbIFNT responsiveness of BUECs in an IFN-signaling pathway-specific manner. In conclusion, physiological and pharmacological stimuli received by the endometrium in vivo were retained as cellular memory in BUECs, persisted in culture, and changed BUEC proliferation/migration and responsiveness to rbIFNT, which are characteristics associated with fertility in cattle.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence for metabolism of creatine by the conceptus, placenta, and uterus for production of adenosine triphosphate during conceptus development in pigs†. 在猪的胚胎发育过程中，胚胎、胎盘和子宫代谢肌酸以产生 ATP 的证据。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-14 DOI: 10.1093/biolre/ioae088

Carli M Lefevre, Joe W Cain, Avery C Kramer, Heewon Seo, Arianna N Lopez, Nirvay Sah, Guoyao Wu, Fuller W Bazer, Gregory A Johnson

{"title":"Evidence for metabolism of creatine by the conceptus, placenta, and uterus for production of adenosine triphosphate during conceptus development in pigs†.","authors":"Carli M Lefevre, Joe W Cain, Avery C Kramer, Heewon Seo, Arianna N Lopez, Nirvay Sah, Guoyao Wu, Fuller W Bazer, Gregory A Johnson","doi":"10.1093/biolre/ioae088","DOIUrl":"10.1093/biolre/ioae088","url":null,"abstract":"In pigs, the majority of embryonic mortality occurs when free-floating conceptuses (embryos/fetuses and associated placental membranes) elongate, and the uterine-placental interface undergoes folding and develops areolae. Both periods involve proliferation, migration, and changes in morphology of cells that require adenosine triphosphate (ATP). We hypothesize that insufficient ATP in conceptus and uterine tissues contributes to conceptus loss in pigs. Creatine is stored in cells as phosphocreatine for ATP regeneration through the creatine-creatine kinase- phosphocreatine pathway. However, the expression of components of this pathway in pigs has not been examined throughout gestation. Results of qPCR analyses indicated increases in AGAT, GAMT, CKM, CKB, and SLC6A8 mRNAs in elongating porcine conceptuses, and immunofluorescence microscopy localized guanidinoacetate N-methyltransferase, creatine kinase M, and creatine kinase B proteins to the trophectoderm of elongating conceptuses, to the columnar chorionic epithelial cells at the bottom of chorioallantoic troughs, and to endometrial luminal epithelium at the tops of the endometrial ridges of uterine-placental folds on Days 40, 60, and 90 of gestation. Guanidinoacetate N-methyltransferase protein is expressed in endometrial luminal epithelium at the uterine-placental interface, but immunostaining is more intense in luminal epithelium at the bottoms of the endometrial ridges. Results of this study indicate that key elements of the pathway for creatine metabolism are expressed in cells of the conceptus, placenta, and uterus for potential production of ATP during two timepoints in pregnancy with a high demand for energy; elongation of the conceptus for implantation and development of uterine-placental folding during placentation.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Provision of choline chloride to the bovine preimplantation embryo alters postnatal body size and DNA methylation†. 为牛植入前胚胎提供氯化胆碱会改变出生后的体型和 DNA 甲基化。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-14 DOI: 10.1093/biolre/ioae092

McKenzie L J Haimon, Eliab Estrada-Cortés, Thiago F Amaral, Hélène Martin, Surawich Jeensuk, Jeremy Block, Daniella Heredia, M Venturini, Camila Santos Rojas, Angela M Gonella-Diaza, Nicolas DiLorenzo, Tracy L Scheffler, Pascal Dufour, Marc-André Sirard, Gabriela Dalmaso de Melo, Ky G Pohler, Peter J Hansen

{"title":"Provision of choline chloride to the bovine preimplantation embryo alters postnatal body size and DNA methylation†.","authors":"McKenzie L J Haimon, Eliab Estrada-Cortés, Thiago F Amaral, Hélène Martin, Surawich Jeensuk, Jeremy Block, Daniella Heredia, M Venturini, Camila Santos Rojas, Angela M Gonella-Diaza, Nicolas DiLorenzo, Tracy L Scheffler, Pascal Dufour, Marc-André Sirard, Gabriela Dalmaso de Melo, Ky G Pohler, Peter J Hansen","doi":"10.1093/biolre/ioae092","DOIUrl":"10.1093/biolre/ioae092","url":null,"abstract":"Choline is a vital micronutrient. In this study, we aimed to confirm, and expand on previous findings, how choline impacts embryos from the first 7 days of development to affect postnatal phenotype. Bos indicus embryos were cultured in a choline-free medium (termed vehicle) or medium supplemented with 1.8 mM choline. Blastocyst-stage embryos were transferred into crossbred recipients. Once born, calves were evaluated at birth, 94 days, 178 days, and at weaning (average age = 239 days). Following weaning, all calves were enrolled into a feed efficiency trial before being separated by sex, with males being slaughtered at ~580 days of age. Results confirm that exposure of 1.8 mM choline chloride during the first 7 days of development alters postnatal characteristics of the resultant calves. Calves of both sexes from choline-treated embryos were consistently heavier through weaning and males had heavier testes at 3 months of age. There were sex-dependent alterations in DNA methylation in whole blood caused by choline treatment. After weaning, feed efficiency was affected by an interaction with sex, with choline calves being more efficient for females and less efficient for males. Calves from choline-treated embryos were heavier, or tended to be heavier, than calves from vehicle embryos at all observations after weaning. Carcass weight was heavier for choline calves and the cross-sectional area of the longissimus thoracis muscle was increased by choline.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141299956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PPARG is dispensable for bovine embryo development up to tubular stages†. PPARG 在牛胚胎发育到管状阶段时是不可或缺的。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-14 DOI: 10.1093/biolre/ioae083

Alba Pérez-Gómez, Leopoldo González-Brusi, Inés Flores-Borobia, Nuria Martínez De Los Reyes, Adolfo Toledano-Díaz, Antonio López-Sebastián, Julián Santiago Moreno, Priscila Ramos-Ibeas, Pablo Bermejo-Álvarez

{"title":"PPARG is dispensable for bovine embryo development up to tubular stages†.","authors":"Alba Pérez-Gómez, Leopoldo González-Brusi, Inés Flores-Borobia, Nuria Martínez De Los Reyes, Adolfo Toledano-Díaz, Antonio López-Sebastián, Julián Santiago Moreno, Priscila Ramos-Ibeas, Pablo Bermejo-Álvarez","doi":"10.1093/biolre/ioae083","DOIUrl":"10.1093/biolre/ioae083","url":null,"abstract":"Following blastocyst hatching, ungulate embryos undergo a prolonged preimplantation period termed conceptus elongation. Conceptus elongation constitutes a highly susceptible period for embryonic loss, and the embryonic requirements during this process are largely unknown, but multiple lipid compounds have been identified in the fluid nourishing the elongating conceptuses. Peroxisome proliferator-activated receptors mediate the signaling actions of prostaglandins and other lipids, and, between them, PPARG has been pointed out to play a relevant role in conceptus elongation by a functional study that depleted PPARG in both uterus and conceptus. The objective of this study has been to determine if embryonic PPARG is required for bovine embryo development. To that aim, we have generated bovine PPARG knock-out embryos in vitro using two independent gene ablation strategies and assessed their developmental ability. In vitro development to Day 8 blastocyst was unaffected by PPARG ablation, as total, inner cell mass, and trophectoderm cell numbers were similar between wild-type and knock-out D8 embryos. In vitro post-hatching development to D12 was also comparable between different genotypes, as embryo diameter, epiblast cell number, embryonic disk formation, and hypoblast migration rates were unaffected by the ablation. The development of tubular stages equivalent to E14 was assessed in vivo, following a heterologous embryo transfer experiment, observing that the development of extra-embryonic membranes and of the embryonic disk was not altered by PPARG ablation. In conclusion, PPARG ablation did not impaired bovine embryo development up to tubular stages.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11402522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141237219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Placental apoptosis increased by hypoxia inducible factor-1 stabilization is counteracted by leptin†. 瘦素可抵消因缺氧诱导因子-1 稳定而增加的胎盘凋亡。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-14 DOI: 10.1093/biolre/ioae095

Nataly de Dios, Rodrigo Riedel, Malena Schanton, Paula Balestrini, Luciano Pérez, Antonio Pérez-Pérez, Tomás Etcheverry, Roberto Casale, Mariana Farina, Víctor Sánchez-Margalet, Julieta Maymó, Cecilia Varone

{"title":"Placental apoptosis increased by hypoxia inducible factor-1 stabilization is counteracted by leptin†.","authors":"Nataly de Dios, Rodrigo Riedel, Malena Schanton, Paula Balestrini, Luciano Pérez, Antonio Pérez-Pérez, Tomás Etcheverry, Roberto Casale, Mariana Farina, Víctor Sánchez-Margalet, Julieta Maymó, Cecilia Varone","doi":"10.1093/biolre/ioae095","DOIUrl":"10.1093/biolre/ioae095","url":null,"abstract":"During pregnancy, apoptosis is a physiological event critical in the remodeling and aging of the placenta. Increasing evidence has pointed toward the relevance of hypoxia as modulator of trophoblast cell death. Previous reports have shown that leptin, a placental cytokine, promotes cell survival in both cell culture and placental explant models. The aim of this work is to establish the role of leptin in apoptosis under hypoxic condition in trophoblast cells. In this study, we evaluated the effect of cobalt chloride, a hypoxia mimicking agent that stabilizes the expression of hypoxia-inducible factor-1 alpha, on Swan-71 and human placental explants. Hypoxia chamber was also used to generate 2% oxygen. Apoptosis was determined by the presence of apoptotic nucleus, fragmentation of DNA and Caspase-3 and PARP-1 cleavage. The pro-apoptotic proteins BAX, BID, BAD, and BAK and the anti-apoptotic effectors BCL-2, B-cell lymphoma-extra-large, and myeloid cell leukemia-1 were also analyzed. We found that hypoxia-inducible factor-1 alpha stabilization increased the appearance of apoptotic nucleus, fragmentation of DNA, and Caspase-3 and PARP-1 cleavage. Hypoxia mimicking conditions enhanced the expression of pro-apoptotic effectors BAX, BID, BAD, and BAK. Hypoxia-inducible factor-1 alpha stabilization also downregulated the level of BCL-2, B-cell lymphoma-extra-large, and myeloid cell leukemia-1. All these apoptotic parameters changes were reversed with leptin treatment. Moreover, we showed that leptin action on apoptosis modulation involves PI3K and MAPK signaling pathways. Obtained data demonstrate that hypoxia-inducible factor-1 alpha stabilization induces apoptosis in human placenta and leptin counteracts this effect, reinforcing its role as a survival cytokine.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141455135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0