Biology of Reproduction最新文献

{"title":"Markers of conceptus development reveal reduced developmental competence in pregnancies derived from lower SCR bulls.","authors":"Florentino P J da-Silva-Junior, Paulo M Bonacim, Ellie G Kidwell, Lillian X Ehresmann, Bo R Harstine, Rafael R Domingues","doi":"10.1093/biolre/ioag233","DOIUrl":"https://doi.org/10.1093/biolre/ioag233","url":null,"abstract":"<p><p>Differences in fertility among bulls are well documented in the field, yet the understanding of the male contributions to in vivo embryonic development and pregnancy outcomes remains ill-defined. This study aimed to evaluate the effect of sire fertility, based on sire conception rate (SCR), on the timing of pregnancy loss and markers of conceptus developmental capacity in vivo. Angus cows were artificially inseminated with semen from Holstein sires classified as HighSCR (n = 55) or LowSCR (n = 104). Day 0 (d0) was defined as the day of AI. Cervical cells were collected on d3, 14, 16, 18, and 20 to assess ISG15 mRNA abundance, and blood was collected on d3 and daily from d14 to d31 to determine progesterone and pregnancy-associated glycoproteins (PAG). Pregnancy outcomes were defined using ISG15 (d14), PAG (d23), and ultrasonography (d31). Pregnancy per AI was greater for HighSCR than LowSCR on d14 (96.4% vs 85.6%; P = 0.05), d23 (81.8% vs 61.5%; P = 0.01), and d31 (69.1% vs 50.0%; P = 0.02). Embryos derived from LowSCR had reduced developmental competence as evidenced by lower ISG15 abundance compared to HighSCR (42.3 ± 4.4 vs 61.6 ± 7.9; P = 0.04) and three-fold greater pregnancy loss particularly when trophoblast differentiation and attachment occurred on ≥d21. Additionally, pregnancy loss prior to d24 was most often (95%) associated with embryonic failure and luteal regression whereas pregnancy loss after d23 most often associated with conceptus failure without luteal regression (85%).</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149223167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RAC1-YAP1-PIEZO1 signaling axis links intrauterine inflammation to uterine hypercontractility and preterm labor.","authors":"Yanmei Bi, Hao Li, Min Diao, Qian Liu, Lu Huang, Yunkai Tao, Yantong Wan, Yi Qu, Xuemei Lin","doi":"10.1093/biolre/ioag209","DOIUrl":"https://doi.org/10.1093/biolre/ioag209","url":null,"abstract":"<p><p>Preterm birth is a leading cause of neonatal morbidity and mortality, yet its underlying mechanisms remain incompletely understood. Our previous studies have demonstrated that the mechanosensitive ion channel PIEZO1 plays a critical role in regulating intracellular calcium levels and amplifying inflammation in uterine smooth muscle (USM) cells, thereby promoting USM contractility and preterm labor. However, the upstream regulatory mechanisms driving PIEZO1 expression during infection-induced preterm labor remain unclear. Here, we established an infection-induced preterm birth mouse model using intrauterine lipopolysaccharide (LPS) injection and assessed USM contractility via ex vivo tension measurements. Western blotting and immunofluorescence were employed to assess the expression and cellular localization of PIEZO1, YAP1, and RAC1 in both uterine tissue and cultured USM cells. Pharmacological inhibitors (Verteporfin for YAP1 and NSC23766 for RAC1) and Rac1 siRNA were utilized to investigate the functional roles and regulatory relationships among these molecules. We found that LPS induced YAP1 dephosphorylation and nuclear translocation, which was inhibited by Verteporfin, reducing PIEZO1 expression and USM contractility, and prolonging gestation. Elevated RAC1 expression in LPS-treated tissues drove YAP1 nuclear translocation and PIEZO1 overexpression. Our findings suggest that RAC1 is involved in regulating YAP1 activation and nuclear localization, which is associated with increased PIEZO1 expression and uterine smooth muscle contractility. Thus, targeting the RAC1-YAP1-PIEZO1 axis might represent a novel strategy for preventing infection-induced preterm birth.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CCDC191 localizes to the sperm head-tail coupling apparatus and is essential for spermiogenesis and male fertility in mice.","authors":"Shanshan Nai, Sijia Jia, Xunshuo Liu, Qingchao Li, Ting Song, Huijie Zhao","doi":"10.1093/biolre/ioag235","DOIUrl":"https://doi.org/10.1093/biolre/ioag235","url":null,"abstract":"","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149191366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Directed conversion of porcine expanded pluripotent stem cells into trophoblast stem cells.","authors":"Chi-Hun Park, Young-Hee Jeoung, JiTao Wang, Bhanu P Telugu","doi":"10.1093/biolre/ioag234","DOIUrl":"https://doi.org/10.1093/biolre/ioag234","url":null,"abstract":"<p><p>Trophoblast stem cells (TSCs) serve as a valuable system for investigating the signaling pathways that regulate extraembryonic lineage commitment. Although trophoblast specification has been extensively characterized in humans and rodents, comparable tools and molecular frameworks remain underdeveloped in pigs, a species of significant agricultural and biomedical relevance. This study identifies defined biochemical conditions that enable efficient conversion of porcine (p) expanded pluripotent stem cells (EPSCs) into induced TSCs (iTSCs). The results demonstrate that combined inhibition of TGF-β/Activin and MEK/ERK signaling is sufficient to induce and maintain core trophoblast regulators, including CDX2, GATA3, KRT7/18, HAND1, and ELF5, while suppressing pluripotency networks. Transcriptomic analyses, benchmarked against porcine in vivo trophoblast reference datasets, indicate that piTSCs closely resemble peri-implantation and term trophoblast populations, confirming successful lineage conversion from the pluripotent state. In vivo, piTSCs formed only small, lineage-restricted lesions lacking ectodermal and endodermal derivatives and exhibiting widespread immunoreactivity for trophoblast markers. In contrast, parental pEPSCs generated teratomas containing derivatives of all three germ layers. Collectively, these findings establish a robust experimental framework for trophoblast induction and characterization in pigs, providing a model to investigate conserved and species-specific mechanisms of trophoblast development and placental biology.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149191325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PBMC-derived RNA signatures show impaired ability to regulate immune responses in women developing preeclampsia†.","authors":"Monica Frøystad, Thor Ueland, Marie Cecilie Paasche Roland, Tove Lekva","doi":"10.1093/biolre/ioag143","DOIUrl":"10.1093/biolre/ioag143","url":null,"abstract":"<p><p>Preeclampsia is a serious pregnancy complication with unclear etiology, making early detection challenging but imperative to improve prognosis. We here examine the potential roles of protein-coding and long non-coding (lnc) RNA from peripheral blood mononuclear cells (PBMCs) in the development of preeclampsia by examining women during pregnancy and 5 years following pregnancy. We isolated PBMCs from women with preeclampsia (N = 37) and normal pregnant controls (N = 215) at 22-24 and 36-38 weeks of gestation, and 5-year follow-up. We performed RNA sequencing at all time points for N = 7 normal pregnant controls and N = 9 women with preeclampsia and explored data manually and using bioinformatics analyses. Spearman correlation was used between lncRNA and preeclampsia-associated markers, while Notch-related RNAs were measured by qPCR. We found dysregulated RNAs at all time points, mostly at 36-38 weeks (491 upregulated, 155 downregulated). Most downregulated RNAs were lncRNA and these correlated positively with P-selectin and negatively with sFlt-1 and myeloperoxidase from the maternal circulation. Overrepresentation analyses and manual data exploration at 36-38 weeks revealed increased activation of adaptive immune functions and dampening of innate immune response, including Notch signaling. NOTCH2NLA/B/C was decreased at 22-24 weeks in the total cohort. In conclusion, circulating protein-coding and lncRNA from women developing preeclampsia broadly indicate enhanced adaptive immune cell activation but dampened innate immune responses. The association of downregulated lncRNAs with markers of endothelial dysfunction and platelet activation may support impaired immune regulation in these women. The function of circulating lncRNAs in women with preeclampsia warrants further study.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1212-1225"},"PeriodicalIF":3.2,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13669408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148418309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stearoyl-CoA desaturase 1-mediated lipid metabolic reprogramming in granulosa cells contributes to follicular dysfunction in non-overweight polycystic ovary syndrome patients†.","authors":"Ningning Xie, Jue Zhou, Wenshan Zeng, Paul James Hardiman, Hye Won Lee, Myeong Soo Lee, Fangfang Wang, Fan Qu","doi":"10.1093/biolre/ioag152","DOIUrl":"10.1093/biolre/ioag152","url":null,"abstract":"<p><p>In women of reproductive age, polycystic ovary syndrome (PCOS) is a widespread endocrine condition that is primarily connected to disrupted lipid metabolism. However, the cell-specific lipidomic signatures and their regulatory mechanisms in ovarian granulosa cells (GCs) of PCOS women remain elusive, particularly across clinical subgroups. We conducted quantitative lipidomics to profile luteinized GC lipid metabolism in PCOS subgroups. Non-overweight PCOS (NOP) patients underwent targeted validation via qRT-PCR and western blotting for lipid metabolism markers. Mechanistic studies employed SCD1-silenced human GC lines and a GC-specific SCD1 knockout mouse model, incorporating long-term fertility assessments. GCs from PCOS women, especially the NOP subgroup, displayed a unique lipidomic signature marked by suppressed biosynthesis and enhanced degradation, contrasting with circulating lipid profiles. Clinically, SCD1 expression correlated with improved embryo quality and implantation rates. In vitro, SCD1 deficiency triggered GC metabolic dysfunction, featuring reduced lipogenesis, elevated lipid peroxidation, iron accumulation, and mitochondrial impairment, which are characteristic of ferroptosis. In vivo, the GC-specific SCD1 deletion model exhibited an accumulation of secondary follicles, increased atresia, and progressive subfertility, alongside disrupted expression of lipid and iron metabolic regulators. Our study provides an integrated evidence chain suggesting that SCD1 acts as a regulator of lipid homeostasis in GCs. SCD1 deficiency contributes to metabolic disturbances that disrupt folliculogenesis, providing a potential mechanistic basis for the subtype-specific follicular dysfunction observed in NOP patients.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1147-1165"},"PeriodicalIF":3.2,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of apical-out human fallopian tube organoids for modeling sperm-epithelium interactions†.","authors":"Chuncheng Lu, Liangdi Su, Zhao Wu, Hongbin Gao, Zhigang Yao, Youzhe Tan, Ao Zhang, Zhuo Wang, Jikun Liu, Wenjie Lai, Hui Zhao","doi":"10.1093/biolre/ioag153","DOIUrl":"10.1093/biolre/ioag153","url":null,"abstract":"<p><p>Elevated sperm DNA fragmentation index (DFI) is closely associated with adverse pregnancy outcomes, although the underlying mechanisms remain unclear. The fallopian tube is a key site for sperm selection and functional regulation, yet whether it differentially responds to sperm with varying DFI levels remains unknown. Reliable in vitro models for investigating sperm-fallopian tube interactions are currently lacking. Therefore, the development of reliable in vitro models to dissect the process of sperm selection by the fallopian tube will provide a new perspective for elucidating the mechanisms high DFI associated infertility. Primary epithelial cells isolated from the human fallopian tube isthmus were used to establish fallopian tube organoids (FTOs), and a suspension culture strategy was applied to reverse epithelial polarity, producing an apical-out configuration. Comprehensive histological, immunofluorescence, ultrastructural, and gene expression analyses confirmed that epithelial differentiation and barrier integrity were maintained following polarity reversal. A co-culture system was subsequently developed to enable direct sperm-epithelium contact. Apical-out FTOs supported sperm adhesion and improved sperm viability by reducing apoptosis and intracellular reactive oxygen species levels. Transcriptomic profiling revealed that normal sperm primarily induced pathways related to immune modulation and extracellular matrix remodeling, whereas high-DFI sperm induced inflammatory responses. Collectively, this study introduces and validates a novel apical-out human FTOs model that overcomes structural limitations of conventional systems. This platform enables relevant investigation of sperm-epithelium interactions and reveals that the tubal epithelium differentially responds to sperm DNA integrity, offering new insight into female tract potential implications in male factor infertility.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1107-1123"},"PeriodicalIF":3.2,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148560691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ifitm3 positively regulates homeostasis of long-term cultured oogonial stem cells derived from Paralichthys olivaceus†.","authors":"Yuqin Ren, Yucong Yang, Guixing Wang, Zengsheng Han, Nuan He, Xiyuan Wang, Yitong Zhang, Ziyang He, Xiaodong Cui, Zhongwei He, Yufeng Liu, Wei Cao, Xiaoyan Zhang, Yufen Wang, Xianjiang Kang, Jilun Hou","doi":"10.1093/biolre/ioag172","DOIUrl":"10.1093/biolre/ioag172","url":null,"abstract":"<p><p>Fish oogonial stem cells (OSCs) are vital for fish reproduction research and germplasm conservation, yet stable long-term in vitro culture of fish OSCs remains a major technical bottleneck. Herein, we established an efficient in vitro culture system (termed L15SP) for OSCs derived from Paralichthys olivaceus, consisting of L-15 medium supplemented with 15% fetal bovine serum, 2 μg/L basic fibroblast growth factor, 2 μg/L leukemia inhibitory factor, 50 μmol/L β-mercaptoethanol, 1% fish serum, and 18 g/L embryo extract protein, cultured at 23°C. This system enabled stable long-term passage of P. olivaceus OSCs. Further investigations revealed that interferon-induced transmembrane protein 3 (Ifitm3) was highly expressed on the membrane of P. olivaceus OSCs. Functional assays demonstrated that Ifitm3 significantly enhanced proliferation, migration, and stemness maintenance of long-term cultured OSCs. Ifitm3 positively modulated the expression of key PI3K-AKT signaling components, including pik3cb, pik3r1, and akt2, to regulate downstream OSC functional genes. Upon pathway activation, Ifitm3 facilitated PIP3 enrichment at the OSC plasma membrane, thereby amplifying PI3K-AKT signaling. In conclusion, Ifitm3 governed OSC biological functions by potentiating PI3K-AKT signal transduction. This study establishes a reliable long-term culture system for fish OSCs and identifies a novel functional marker for OSC identification, providing valuable support for fish germplasm preservation and genetic breeding.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1166-1183"},"PeriodicalIF":3.2,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148763268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TREM2 deficiency attenuates endometriosis progression by inhibiting M2 polarization of SpMs and suppressing SIRPα to enhance phagocytosis†.","authors":"Qinglin Mo, Yichun Lin, Liwei Hao, Leyi Zhang, Zilu Zhou, Ruoshui Chang, Wei Huang, Lei Huang, Yunxiao Zhou, Yuxiao Yang, Zhuoran Liu, Wei Zhu, Xiaolei Xue, Sha Wu","doi":"10.1093/biolre/ioag159","DOIUrl":"10.1093/biolre/ioag159","url":null,"abstract":"<p><p>Endometriosis (EMS) is characterized by a disrupted peritoneal immune microenvironment where alternatively activated anti-inflammatory (M2) macrophage polarization and impaired phagocytosis promote lesion survival. Single-cell RNA-sequencing (ScRNA-seq) of the peritoneal macrophages from an EMS patient revealed elevated triggering receptor expressed on myeloid cells 2 (TREM2) and signal regulatory protein alpha (SIRPα). The expression of TREM2 and SIRPα is positively correlated, and each is positively correlated with estrogen response. A mouse EMS model was established by intraperitoneal injection of estrogen primed mouse endometrial fragments into recipient female mice. In vitro estrogen treatment of RAW 264.7 cells indicated increased TREM2/SIRPα expression and enhanced phagocytosis of lesion cells derived from wild-type (WT) EMS ectopic lesions. Peritoneal macrophages from mice with EMS were analyzed using flow cytometry for the expression of TREM2 and SIRPα. The assessment of F4/80, CD206, and SIRPα expressing cells within the lesions was performed through the implementation of multicolor immunohistochemistry (mIHC). Small peritoneal macrophages (SpMs, F4/80low CD11blow) were markedly increased in the EMS model. Trem2 knockout (KO) mice, as the recipient, showed smaller ectopic lesion size and less lesion formation. TREM2 deficiency inhibited SpM polarization into M2 and downregulated their SIRPα expression, while enhancing phagocytic activity. Mechanistically, estrogen pretreatment upregulated TREM2, which correlated with SIRPα upregulation and impaired phagocytosis. Under estrogen exposure, TREM2 knockdown RAW 264.7 cells potentiated phagocytosis. Thus, TREM2 is an important contributing pathogenic factor in promoting EMS by enhancing M2 polarization and suppressing phagocytosis via SIRPα upregulation. Our data suggest that blocking TREM2 may benefit EMS patient.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1134-1146"},"PeriodicalIF":3.2,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148677072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"Old and new\" frontiers in embryo viability assessment: current practices and introduction of emerging approaches.","authors":"Sareena Rayne Singh, Madeline Abass, Jakub Bartlewski, Joanna Kochan, Maciej Murawski, Jakub Wyroba, Pawel M Bartlewski","doi":"10.1093/biolre/ioag147","DOIUrl":"10.1093/biolre/ioag147","url":null,"abstract":"<p><p>Reliable assessment of embryo viability remains a critical yet challenging component of assisted reproductive technologies (ART). Traditional methods, including morphological grading and preimplantation genetic testing for aneuploidy (PGT-A), are either subjective, invasive, or resource intensive. Consequently, non-invasive approaches, including analysis of spent culture medium (SCM) and digital image-based analytical methods, emerged as promising alternatives to support clinical decision-making, though their reliability varies. SCM analyses can detect embryonic DNA and metabolites, but their outcomes are constrained by potential contamination with non-embryonic material and inconsistent amplification efficiency. In contrast, image-based assessment combined with machine learning (ML) enables quantitative, repeatable, and transparent evaluation of embryo architecture. Segmental image analysis of grayscale bitmaps allows for extraction of interpretable phototextural metrics, which have been correlated with developmental competence and ploidy status of embryos. Recent applications of ML-assisted techniques demonstrate comparable or superior predictive performance relative to PGT-A and expert embryologists, highlighting their potential as cost-effective and ethically favorable alternatives. Continued refinement of image acquisition minimizing artifacts, optimizing quality and resolution, and focusing on key regions such as the trophectoderm and inner cell mass, may further enhance their diagnostic sensitivity and specificity. Overall, computerized assessment of embryo microphotographs represents a scalable, non-invasive strategy that can complement or even partially replace invasive testing. By leveraging structured, interpretable image data, algorithms such as r-Algo 2.0 (discriminative image-analysis software) exemplify the emerging role of computational tools in advancing diagnostic precision and objectivity in ART. Highlights  Current methods of embryo viability assessment lack clinical reliabilityNon-invasive image analysis supports accurate embryo quality assessmentTransparent algorithms enhance repeatability of image-analysis techniquesImage-based embryo assessments may complement or reduce the need for PGT-AImaging refinements can further enhance its diagnostic sensitivity and specificity.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1013-1031"},"PeriodicalIF":3.2,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13669403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148444315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}