Biology of Reproduction最新文献_第2页

Metformin alters liver metabolism to sustain egg production in the reproductively aging broiler breeder hen†. 二甲双胍可改变肝脏代谢，以维持繁殖力衰老的肉用种鸡的产蛋量。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-07-13 DOI: 10.1093/biolre/ioaf072

Evelyn Anne Weaver, Nathan Patrick Connolly, Tae Hyun Kim, Ramesh Ramachandran

{"title":"Metformin alters liver metabolism to sustain egg production in the reproductively aging broiler breeder hen†.","authors":"Evelyn Anne Weaver, Nathan Patrick Connolly, Tae Hyun Kim, Ramesh Ramachandran","doi":"10.1093/biolre/ioaf072","DOIUrl":"10.1093/biolre/ioaf072","url":null,"abstract":"The liver is critical in avian reproduction as it is the primary site of de novo lipogenesis and yolk precursor synthesis. Broiler breeder hens, the parents of commercial broiler chickens, have poor reproductive efficiency due to declining egg production from 45 weeks of age. We found that metformin increases fertile egg production in the aging broiler breeder hen, which was correlated with reduced body weight, reduced fat pad weight, and altered reproductive hormone profiles. This study aimed to characterize the liver transcriptome of the same broiler breeder hens supplemented with metformin in the diet at 0 or 75 mg/kg body weight for 40 weeks (25-65 weeks of age; n = 45 hens/treatment). Liver tissue was collected from a subset of hens (n = 12 hens/treatment group) at 65 weeks of age, and RNA was extracted and sequenced using next-generation sequencing. Differential gene abundance analysis revealed that metformin treatment led to significant changes in gene expression. Further transcriptomic analysis highlighted increased expression of genes related to estrogen-stimulated yolk precursor synthesis, insulin-stimulated de novo lipogenesis, and AMPK-mediated glucose homeostasis. Quantitative PCR analysis revealed increased expression of ESR1, APOB, APOV1, VTG2, ADIPOQ, ADIPOR2, and ACACA mRNA and decreased expression of PCK1 mRNA while plasma triglyceride and non-esterified fatty acid levels were lower in metformin-treated animals compared to controls. The present study suggests that metformin supplementation supports prolonged egg production in aging broiler breeder hens by sustaining yolk precursor and fatty acid synthesis that are typically diminished in aging broiler breeder hens.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"169-181"},"PeriodicalIF":3.1,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved evaluation of mitochondrial membrane potential in bovine spermatozoa using JC-1 with flow cytometry†. 流式细胞术改进JC-1对牛精子线粒体膜电位的评价。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-07-13 DOI: 10.1093/biolre/ioaf081

Abigail L Zezeski, Lauren E Hamilton, Thomas W Geary

{"title":"Improved evaluation of mitochondrial membrane potential in bovine spermatozoa using JC-1 with flow cytometry†.","authors":"Abigail L Zezeski, Lauren E Hamilton, Thomas W Geary","doi":"10.1093/biolre/ioaf081","DOIUrl":"10.1093/biolre/ioaf081","url":null,"abstract":"One of the most popular methods for measuring mitochondrial membrane potential (MMP) via conventional flow cytometry in spermatozoa is through the use of the fluorescent dye 5,5,6,6'-tetrachloro-1,1',3,3'tetraethylbenzimi-dazoylcarbocyanine iodide (JC-1). The JC-1 dye is unique in that it will fluoresce green (525 nm, monomers) or red-orange (595 nm, J-aggregates) when excited to indicate depolarized or polarized mitochondria. While JC-1 can be multiplexed with viability dyes such as propidium iodide and SYBR 14 for microscopy, doing so for conventional flow cytometry has proved difficult due to overlap of the emission spectra between dyes. The objective of this protocol is to improve the accuracy of JC1 J-aggregate quantification in conventional flow cytometry by using a 405 nm or 532 nm laser for excitation coupled with the viability dye calcein violet (CV). Quantification of live J-aggregates when excited with a 405 nm or 532 nm laser is more strongly correlated to progressive motility (405 nm: r = 0.73, P < 0.0001; 532 nm: r = 0.52, P = 0.0002) and viability (405 nm: r = 0.93, P < 0.0001; 532 nm: r = 0.74, P < 0.0001) than quantification with the traditional 488 nm laser excitation and dual emission of 525 nm and 595 nm (progressive motility: r = 0.05, P = 0.72; viability: r = 0.21, P = 0.14). The use of a 405 nm or a 532 nm laser requires no compensation. This allows for clear identification of the polarized J-aggregates and, when combined with CV, may help identify which spermatozoa have the greatest fertilization potential.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"141-153"},"PeriodicalIF":3.1,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The MMP2 and MMP9-specific inhibitor SB-3CT significantly decreases blood pressure in pre-eclampsia model rats. MMP2和mmp9特异性抑制剂SB-3CT显著降低子痫前期模型大鼠血压。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-07-13 DOI: 10.1093/biolre/ioaf075

Bowei Li, Jianfang Luo, Wanxing Zhou

{"title":"The MMP2 and MMP9-specific inhibitor SB-3CT significantly decreases blood pressure in pre-eclampsia model rats.","authors":"Bowei Li, Jianfang Luo, Wanxing Zhou","doi":"10.1093/biolre/ioaf075","DOIUrl":"10.1093/biolre/ioaf075","url":null,"abstract":"Although uterine matrix metalloproteinases (MMPs) are known to play a role in the development of pre-eclampsia (PE), it remains unclear whether increased levels of serum MMPs are the pathogenesis underlying pregnancy-induced hypertension (PIH). This study investigated whether serum MMP2 and MMP9 contributes to PIH pathogenesis using a specific inhibitor, SB-3CT. Twenty-five nine-week-old pregnant rats were randomly divided into five groups: SHAM (normal pregnancy), RUPP (PE model), and three SB-3CT intervention groups (25, 50, 75 mg/kg/d). After 7 days of intraperitoneal injections, carotid artery blood pressure was measured. Serum MMP2, MMP9, tissue inhibitor of metalloproteinase-1 (TIMP1), endothelin-1 (ET-1), angiotensin II (Ang II), and endothelial nitric oxide synthase (eNOS) levels were tested by ELISA. Vascular wall changes in cross-sections of the aorta abdominalis were observed using H&E-staining and the activities of MMP2 and MMP9 in the aorta abdominalis were tested using gelatin zymography. There were significant increases in blood pressure, serum MMP2 and MMP9 levels, and activities of MMP2 and MMP9 in the aorta abdominalis, along with notable vascular remodeling in the RUPP group. After the SB-3CT intervention, increased blood pressure was relieved and vascular remodeling improved, while MMP2 and MMP9 levels and activities were reduced. In summary, specific inhibition of MMP2 and MMP9 can decrease blood pressure in a PIH model. This indicates that increased MMP2 and MMP9 in maternal serum might contribute to the pathogenesis of PIH.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"209-219"},"PeriodicalIF":3.1,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endoplasmic reticulum stress: an emerging therapeutic target for preeclampsia†. 内质网应激：子痫前期的新兴治疗靶点。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-07-13 DOI: 10.1093/biolre/ioaf071

Mengting Chen, Yafang Jin, Xiaohui Cao

引用次数: 0

Testing the function of phosphoprotein phosphatases modified by luteinizing hormone signaling in mouse ovarian follicles: an update†. 测试小鼠卵泡中被黄体生成素信号改变的 PPP 家族磷酸酶的功能：最新进展。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-07-13 DOI: 10.1093/biolre/ioaf076

Jeremy R Egbert, Corie M Owen, Tracy F Uliasz, Deborah Kaback, Siu-Pok Yee, Laurinda A Jaffe

引用次数: 0

Endogenous regulation of behavior and reproductive physiology in a resident passerine songbird†. 雀形目鸣禽行为和生殖生理的内源性调控

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-07-13 DOI: 10.1093/biolre/ioaf073

Shirin Fatima, Sayantan Sur, Jyoti Tiwari, Sangeeta Rani, Shalie Malik

{"title":"Endogenous regulation of behavior and reproductive physiology in a resident passerine songbird†.","authors":"Shirin Fatima, Sayantan Sur, Jyoti Tiwari, Sangeeta Rani, Shalie Malik","doi":"10.1093/biolre/ioaf073","DOIUrl":"10.1093/biolre/ioaf073","url":null,"abstract":"Seasonal timing of reproductive events requires the interaction of the circannual clock and environmental cues. Many avian species exhibit robust circannual rhythms in controlled environments. However, the molecular changes preceding changes in physiology and behavior are poorly understood. The spotted munia (Lonchura punctulata) is an ideal experimental animal to investigate this question as it shows a strong annual cyclicity under prolonged captive conditions. In the current experiment, birds (18 males + 18 females) were maintained under equinox photoperiod (12L:12D, L = light, ~1.86 W/m2; D = dark, <0.00014 W/m2) with constant temperature (22 ± 2°C) and humidity (58 ± 2%) for ~10 months. Based on gonadal status, we identified pre-breeding, breeding, and onset of regression phases and measured body weight, histological changes, active and sleep behavior, and hypothalamic gene expressions. Body fattening, gonadal recrudescence, and organ-specific lipid accumulation were observed during the breeding phase. Increased allopreening behavior coupled with reduced sleep suggested increased social interaction and nighttime vigilance during the reproductive period. The elevated hypothalamic Gonadotropin-Releasing Hormone expression, plasma testosterone, and corticosterone levels during the pre-breeding phase prepared the birds for upcoming reproductive processes. Overall, our data provide evidence of endogenous molecular changes under constant environmental conditions that might inform conserved mechanisms across species.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"154-168"},"PeriodicalIF":3.1,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A mouse model engineered to spatiotemporally control Cre expression in progesterone receptor positive cells†. 黄体酮受体阳性细胞时空调控Cre表达的小鼠模型

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-07-13 DOI: 10.1093/biolre/ioaf090

Elvis Quiroz, Ryan M Marquardt, Shu-Yun Li, Artiom Gruzdev, David Cunefare, Charan Ganta, San-Pin Wu, John P Lydon, Francesco J DeMayo

{"title":"A mouse model engineered to spatiotemporally control Cre expression in progesterone receptor positive cells†.","authors":"Elvis Quiroz, Ryan M Marquardt, Shu-Yun Li, Artiom Gruzdev, David Cunefare, Charan Ganta, San-Pin Wu, John P Lydon, Francesco J DeMayo","doi":"10.1093/biolre/ioaf090","DOIUrl":"10.1093/biolre/ioaf090","url":null,"abstract":"The Cre/loxP system is widely used for site-specific genetic manipulation in mice. The PgrCre mouse model, where Cre recombinase is driven by the progesterone receptor promoter, is commonly used for gene ablation in Pgr-positive uterine cells. However, the PgrCre is active in the neonatal uterus and does not allow temporal control. To enhance the functionality of the PgrCre mouse, we generated and characterized an inducible PgriCreERT2 mouse, in which iCreERT2 is inserted downstream of the endogenous Pgr promoter. PgriCreERT2 mice crossed with Rosa26-CAG-LSL-Sun1-sfGFP-myc reporter mice demonstrated tamoxifen-dependent recombination in uterine stromal fibroblasts and a subset of epithelial cells. Tamoxifen-induced ablation of PGR expression was accomplished by crossing PgriCreERT2 mice with the Pgrflox/flox mice. Resulting PgriCreERT2/Pgr:flox and Pgrflox/flox mice were treated with tamoxifen or oil vehicle daily for 3 days and assayed for fertility 1 month after treatment. Tamoxifen-treated PgriCreERT2/Pgr:flox mice exhibited implantation failure, dysregulation of uterine epithelial and stromal cell proliferation, and loss of decidualization response with no impact on ovulation or embryo transport. A 6-month breading trial demonstrated that 4/6 tamoxifen-treated PgriCreERT2/Pgr:flox mice were completely infertile, and the remaining 2/6 delivered only three total pups each near the end of the trial. In contrast, tamoxifen-treated Cre-negative females were fertile with normal uterine receptivity compared to vehicle controls, indicating that tamoxifen administration with a month-long recovery period did not impair pregnancy. Together, these data demonstrate the utility of this inducible PgriCreERT2 mouse model for spatiotemporally controlled gene ablation in Pgr-positive cells of the uterus.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"83-96"},"PeriodicalIF":3.1,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bovine pregnancy diagnosis crosses the great divide and into the future of reproductive management in cattle. 牛妊娠诊断跨越了巨大的鸿沟，进入了牛生殖管理的未来。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-07-13 DOI: 10.1093/biolre/ioaf136

Matthew C Lucy

引用次数: 0

A bovine 3D endometrium-on-a-chip reveals the role of conceptus-derived proteins, CAPG and PDI, in conceptus-endometrial communication. 牛三维子宫内膜芯片揭示了孕源性因子CAPG和PDI在孕-子宫内膜通信中的作用。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-07-13 DOI: 10.1093/biolre/ioaf077

Haidee Tinning, Elton J R Vasconcelos, Dapeng Wang, Niamh Forde

{"title":"A bovine 3D endometrium-on-a-chip reveals the role of conceptus-derived proteins, CAPG and PDI, in conceptus-endometrial communication.","authors":"Haidee Tinning, Elton J R Vasconcelos, Dapeng Wang, Niamh Forde","doi":"10.1093/biolre/ioaf077","DOIUrl":"10.1093/biolre/ioaf077","url":null,"abstract":"Early embryo loss affects all mammalian species, including humans, and agriculturally important food-producing mammals such as cattle. The developing conceptus (embryo and extraembryonic membranes) secretes proteins that can modify the endometrium and can be critical for early pregnancy processes, such as maternal recognition of pregnancy (MRP) or enhancing uterine receptivity to implantation. For example, a competent bovine conceptus secretes interferon tau (IFNT) to initiate MRP. The bovine conceptus also secretes other proteins at the time of MRP, including CAPG and PDI, which are highly conserved among placental mammals. We have previously shown that these proteins act upon the endometrium to modulate receptivity, embryo development, and implantation in species with different implantation strategies (humans and cattle). We hypothesize that developing a novel 3D bovine endometrium-on-a-chip system will enhance our understanding of the role of conceptus-derived factors in altering the endometrium and/or uterine luminal fluid (ULF) secretion. Here, we have developed a 3D bovine endometrium-on-a-chip system, comprising both stromal and epithelial cell culture combined with culture medium flow. This system better mimics the in vivo endometrium, and endometrial exposure to conceptus-derived factors, than conventional 2D endometrial cell culture. We have demonstrated that the conceptus-derived proteins, CAPG and PDI, modulate the endometrial transcriptome and secretory response to promote pathways associated with early pregnancy and alter ULF composition. This work highlights the critical need for more robust and in vivo-like culture systems to study endometrial-conceptus interactions in vitro to further investigate the role of conceptus-derived factors for pregnancy success.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"61-82"},"PeriodicalIF":3.1,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12260497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of cholesterol/AlkB homolog 1/Integrin alpha 2b in thrombosis in the placental vessels of preeclampsia†. 胆固醇/ALKBH1/ITGA2B在子痫前期胎盘血管血栓形成中的作用。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-07-13 DOI: 10.1093/biolre/ioaf067

Yingying Zhang, Eryun Zhang, Ling Li, Yongwei Ren, Jinqiu Zhang, Qiutong Zheng, Minya Sun, Shaojie Zhao, Yugui Cui, Ying Gu, Zhice Xu

{"title":"The role of cholesterol/AlkB homolog 1/Integrin alpha 2b in thrombosis in the placental vessels of preeclampsia†.","authors":"Yingying Zhang, Eryun Zhang, Ling Li, Yongwei Ren, Jinqiu Zhang, Qiutong Zheng, Minya Sun, Shaojie Zhao, Yugui Cui, Ying Gu, Zhice Xu","doi":"10.1093/biolre/ioaf067","DOIUrl":"10.1093/biolre/ioaf067","url":null,"abstract":"The risk of multiple placental thrombosis is significantly elevated in preeclampsia, and the vascular endothelium plays a vital role in coagulation processes through the secretion of Integrin alpha 2b, von Willebrand factor, and tissue factor. However, the underlying pathological mechanisms remain unclear. In this study, placental blood vessels were collected from both preeclampsia and normal pregnancies. Protein levels of Integrin alpha 2b were found to be up-regulated in preeclampsia samples compared to controls, indicating enhanced coagulation function in preeclampsia placental tissue. Additionally, higher cholesterol levels of plasma and lower AlkB homolog 1 expression in placental blood vessels of preeclampsia were found, overall DNA N6-methyldeoxyadenosine levels were up-regulated. Interestingly, the DNA N6-methyldeoxyadenosine level at the Integrin alpha 2b promoter was decreased. Cholesterol overload experiments in human umbilical vein endothelial cells demonstrated that cholesterol regulates the protein expression of AlkB homolog 1 and Integrin alpha 2b in a concentration-dependent manner. Furthermore, Integrin alpha 2b protein expression was increased following AlkB homolog 1 knockout, but no changes were observed when cholesterol was incubated with AlkB homolog 1 knockout cells. These findings provide critical insights into the roles of cholesterol levels, AlkB homolog 1, and Integrin alpha 2b in coagulation function within placental blood vessels. This information may contribute to further investigations of potential therapeutic targets and early prevention strategies for placental thrombosis in preeclampsia.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"199-208"},"PeriodicalIF":3.1,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0