Biology of Reproduction最新文献_第2页

Microplastics and impaired male reproductive health-exploring biological pathways of harm: a narrative review. 微塑料和男性生殖健康受损-探索危害的生物学途径：叙述回顾。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-06-15 DOI: 10.1093/biolre/ioaf054

Naina Kumar, Mishu Mangla

{"title":"Microplastics and impaired male reproductive health-exploring biological pathways of harm: a narrative review.","authors":"Naina Kumar, Mishu Mangla","doi":"10.1093/biolre/ioaf054","DOIUrl":"10.1093/biolre/ioaf054","url":null,"abstract":"Introduction: Microplastics, pervasive environmental pollutants, have emerged as significant health hazards with growing evidence linking them to impaired male reproductive health. Microplastics can enter the human body through ingestion, inhalation, and dermal absorption and, once internalized, can induce oxidative stress, inflammation, endocrine disruption, and cellular damage leading to impaired male reproductive health. The present narrative review explores the biological pathways through which microplastics impair male reproductive health, focusing on their direct and systemic effects.Methodology: A comprehensive literature search spanning up to February 2025 was conducted across electronic databases, including PubMed, Scopus, Web of Science, and Google Scholar. Search terms such as \"microplastic exposure,\" \"male infertility,\" \"male reproductive health,\" \"oxidative stress,\" \"endocrine disruption,\" \"spermatogenesis,\" \"inflammation,\" and \"reproductive toxicity\" were employed to identify relevant studies published in peer-reviewed journals, books, and reputable conference proceedings. Inclusion criteria were limited to articles written in English that focused on the biological pathways linking MP exposure to impaired male reproductive health. Priority was given to review articles, original research papers, and meta-analyses. Extracted information was systematically organized to provide a narrative synthesis.Conclusion: Current evidence suggests that microplastics may impair male reproductive health through mechanisms like oxidative stress, hormonal disruption, inflammation, and cellular damage. However, the lack of human studies highlights the urgent need for robust research to clarify their impact on human male infertility. Furthermore, this review underscores the necessity for continued research to elucidate molecular mechanisms, inform preventative strategies, and guide regulatory policies addressing microplastic pollution and its health implications.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1028-1038"},"PeriodicalIF":3.1,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kynurenine, a derivative of tryptophan, inhibits progesterone biosynthesis in porcine granulosa luteal cells through Aryl hydrocarbon receptor-mediated downregulation of GATA-binding protein 4, 6, and CCAAT/enhancer-binding protein beta†. 犬尿氨酸是色氨酸的衍生物，通过ahr介导的GATA4、GATA6和CEBPB的下调抑制猪黄体颗粒细胞中的孕酮生物合成。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-06-15 DOI: 10.1093/biolre/ioaf031

Shiying Liao, Jinhua Cheng, Weimin Zhao, Chaohui Dai, Yanfeng Fu, Bixia Li, Yanfei Deng, Hui Li

{"title":"Kynurenine, a derivative of tryptophan, inhibits progesterone biosynthesis in porcine granulosa luteal cells through Aryl hydrocarbon receptor-mediated downregulation of GATA-binding protein 4, 6, and CCAAT/enhancer-binding protein beta†.","authors":"Shiying Liao, Jinhua Cheng, Weimin Zhao, Chaohui Dai, Yanfeng Fu, Bixia Li, Yanfei Deng, Hui Li","doi":"10.1093/biolre/ioaf031","DOIUrl":"10.1093/biolre/ioaf031","url":null,"abstract":"Kynurenine (KYN) is a primary tryptophan derivative found in the human body and fermented foods. Previous studies have shown that KYN is an aryl hydrocarbon receptor (AHR) agonist and is important in regulating various physiological activities, including female reproduction. Progesterone is a vital steroid hormone that facilitates embryo implantation and maintains pregnancy. However, whether KYN affects its biosynthesis remains unclear. To gain understanding, in vitro luteinized porcine granulosa luteal (pGL) cells were treated with KYN. The results showed that KYN disrupted progesterone biosynthesis by decreasing the expression of steroidogenic acute regulatory protein (STAR) and 3beta-hydroxysteroid dehydrogenase (HSD3B) in pGL cells. In addition, the expression of three transcription factors of STAR and HSD3B (GATA4, GATA6, and CEBPB) decreased after KYN treatment. Furthermore, the AHR blockade results showed comparable effects to those of KYN treatment, and subsequent knockdown experiments confirmed these results. These findings suggest that KYN inhibits progesterone biosynthesis in pGL cells by downregulating GATA4, GATA6, and CEBPB expression through AHR. Thus, our results showed for the first time a previously unknown connection between KYN and progesterone biosynthesis.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1200-1212"},"PeriodicalIF":3.1,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diverse roles of stress-responsive RNP granules in oogenesis and infertility. 应激反应性RNP颗粒在卵子发生和不孕症中的多种作用。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-06-15 DOI: 10.1093/biolre/ioaf057

M Rebecca Glineburg, Carolee Nguyen

{"title":"Diverse roles of stress-responsive RNP granules in oogenesis and infertility.","authors":"M Rebecca Glineburg, Carolee Nguyen","doi":"10.1093/biolre/ioaf057","DOIUrl":"10.1093/biolre/ioaf057","url":null,"abstract":"Effectively responding to cellular stress (e.g., nutrient deprivation, oxidative stress) is essential for cell and organismal survival. A protective mechanism is especially critical in developing oocytes, where a prolonged quiescent state and the inability to divide render oocytes highly susceptible to accumulating stress that can result in cell death if unaddressed. Despite the common view that stress granules are the primary stress-responsive ribonucleoprotein granule, accumulating evidence shows that in ovaries, other ribonucleoprotein granules also uniquely mediate gene regulation in response to stress. Here, we review recent insights into ribonucleoprotein granule dynamics and ribonucleoprotein granule protein function during stress in the context of oogenesis among both invertebrates and vertebrates, with an emphasis on insights from Drosophila and mice. We also discuss roles for stress-responsive ribonucleoproteins in maintaining stem cell populations and complicating fertility treatments. By exploring how stress-induced ribonucleoprotein dynamics can impact oogenesis, both positively and negatively, we can better understand how stress contributes to reduced fecundity and infertility. We conclude by offering key research questions that can drive the next generation of insights.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1039-1053"},"PeriodicalIF":3.1,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The roles of AKT isoforms in decidualization and embryo implantation using a Progesterone Receptor-Cre mouse model†. AKT同种异构体在PGR-Cre小鼠模型的去个体化和胚胎着床中的作用。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-06-15 DOI: 10.1093/biolre/ioaf062

Pascal Adam, François Fabi, Sophie Parent, Léa-Isabelle Renaud, Monique Cadrin, Eric Asselin

{"title":"The roles of AKT isoforms in decidualization and embryo implantation using a Progesterone Receptor-Cre mouse model†.","authors":"Pascal Adam, François Fabi, Sophie Parent, Léa-Isabelle Renaud, Monique Cadrin, Eric Asselin","doi":"10.1093/biolre/ioaf062","DOIUrl":"10.1093/biolre/ioaf062","url":null,"abstract":"Implantation is a complex process requiring a prepared, receptive endometrium, reliant on synchronized decidualization of stromal cells. During this process, cell proliferation and apoptosis are tightly regulated by signaling factors, including the survival and proliferation of the PI3K/AKT pathway. The three AKT isoforms each play distinct physiological roles, but their specific functions in endometrial cell survival and apoptosis remain unclear. We hypothesize that for successful implantation, each AKT isoform has distinct roles in the endometrium during decidualization, which varies throughout the process. To explore this, we developed a unique PGR-Cre tissue-specific mouse model with single and combined knockouts (KO) of each AKT isoform. Using artificial decidualization during pseudopregnancy and normal gestation, we investigated the specific activity of each AKT isoform and their downstream targets to assess the role of AKT pathway. Our results showed that the AKT1-2 KO genotype failed to decidualize during pseudopregnancy and exhibited a reduced number of implantation sites. Interestingly, AKT3 was hyperphosphorylated in the AKT1-2 KO mice and emerged as the primary isoform active throughout decidualization, specifically signaling through GSK3B. This study suggests distinct yet partially redundant roles for AKT1 and AKT2 during decidualization and embryo implantation. We propose that the AKT pathway plays significant role in fertility, and a deeper understanding of its involvement in decidualization could lead to improved strategies for addressing fertility issues. These findings highlight the importance of AKT activity in the cellular and molecular regulation of mouse fertility.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1134-1147"},"PeriodicalIF":3.1,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel insights into human CRISP2: localization in reproductive tissues and sperm, and molecular characterization†. 对人类CRISP2的新见解：在生殖组织和精子中的定位，以及分子表征。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-06-15 DOI: 10.1093/biolre/ioaf051

Thibault Masai, Amandine Delnatte, Marie Dendievel, Denis Nonclercq, Annica Frau, Jean-François Simon, Vanessa Arcolia, Ruddy Wattiez, Baptiste Leroy, Patricia S Cuasnicu, Pascale Lybaert, Elise Hennebert

{"title":"Novel insights into human CRISP2: localization in reproductive tissues and sperm, and molecular characterization†.","authors":"Thibault Masai, Amandine Delnatte, Marie Dendievel, Denis Nonclercq, Annica Frau, Jean-François Simon, Vanessa Arcolia, Ruddy Wattiez, Baptiste Leroy, Patricia S Cuasnicu, Pascale Lybaert, Elise Hennebert","doi":"10.1093/biolre/ioaf051","DOIUrl":"10.1093/biolre/ioaf051","url":null,"abstract":"CRISP2 is enriched in the male reproductive system of mammals and plays roles in spermatogenesis, sperm motility, and fertilization. Although extensively investigated in rodents and boars, human CRISP2 (hCRISP2) remains poorly studied, particularly concerning its localization in testicular and epididymal tissues and its molecular features. In this study, we used immunofluorescence to determine the localization of hCRISP2 in testis, epididymis, and ejaculated sperm. While no expression was observed in the epididymal epithelium, hCRISP2 was detected at different stages during spermatogenesis. Specifically, hCRISP2 was found in the nucleus of primary spermatocytes and of both round and early elongated spermatids. In elongated spermatids, it was additionally observed in the cytoplasm, the flagellum, and the equatorial segment of the acrosome (EqS). The presence of aggregated material with hCRISP2 immunoreactivity in the apical pole of Sertoli cells suggests that most of the hCRISP2 involved in spermatogenesis is phagocytized by these cells during spermiation. In ejaculated sperm, hCRISP2 was found in the cytoplasmic droplet, flagellum, and EqS, consistent with its described roles in sperm motility and gamete fusion. Native and denaturing electrophoresis combined with western blot analyses depicted the ability of hCRISP2 to form stable high molecular weight complexes, and mass spectrometry revealed that these complexes likely consist exclusively of hCRISP2. Furthermore, we showed that hCRISP2 undergoes only limited post-translational modifications. These findings shed light into the dynamic localization of hCRISP2 throughout spermatogenesis and in ejaculated sperm, as well as its molecular features, enhancing our understanding of its functional roles and relevance for male fertility.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1167-1184"},"PeriodicalIF":3.1,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of sika deer oviduct epithelial cell-derived extracellular vesicles on oocytes and parthenogenetic embryos†. 梅花鹿输卵管上皮细胞来源的细胞外囊泡对卵母细胞和孤雌胚胎的影响。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-06-15 DOI: 10.1093/biolre/ioaf048

Bing Hu, Shu-Ming Shi, Xian-Feng Yu, Yu-Yan He, Zhi-Chao Chi, Lai-Ming Tian, Guan-Lin Jia, Ilkeun Kong, Yong-Xun Jin, Ming-Jun Zhang

{"title":"The effects of sika deer oviduct epithelial cell-derived extracellular vesicles on oocytes and parthenogenetic embryos†.","authors":"Bing Hu, Shu-Ming Shi, Xian-Feng Yu, Yu-Yan He, Zhi-Chao Chi, Lai-Ming Tian, Guan-Lin Jia, Ilkeun Kong, Yong-Xun Jin, Ming-Jun Zhang","doi":"10.1093/biolre/ioaf048","DOIUrl":"10.1093/biolre/ioaf048","url":null,"abstract":"Oviducts contain various nutrients that provide energy during oocyte development. This study aimed to improve the efficiency of in vitro reproduction using extracellular vesicles (EVs) produced by the oviduct epithelial cells of sika deer (Cervus nippon). Surprisingly, the uptake of deer oviduct epithelial cell extracellular vesicles (DOEC-EVs) by cumulus-oocyte complexes, which were encapsulated by dense cumulus cells (CCs), occurred only in CCs during maturation. Therefore, we hypothesized that DOEC-EVs are transported to oocytes through CCs to exert their effects. We first investigated the effects of DOEC-EVs on the expansion capacity of the cumulus-oocyte complexes, as well as cell cycle progression, proliferation, apoptosis, and lactate and pyruvate levels in CCs, and examined reactive oxygen species levels, mitochondrial function, and key gene expression. The results showed that DOEC-EVs regulated cell cycle progression, promoted proliferation, reduced apoptosis, and improved antioxidant capacity and glycolysis, and through the oocyte first polar body excretion rate, reactive oxygen levels, and mitochondrial membrane potential, it was shown that CC promoted in vitro oocyte maturation, improved the antioxidant capacity and mitochondrial function of oocytes, and promoted parthenogenetic embryo development. These results suggest that DOEC-EVs improve the efficiency of oocyte development in deer in vitro by acting on CCs, laying the foundation for further research on in vitro deer reproduction.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1148-1166"},"PeriodicalIF":3.1,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HMGB1 promotes immune abnormalities in preeclampsia by recruiting monocyte/decidual macrophages and inducing M1 polarization†. HMGB1通过募集单核/蜕膜巨噬细胞和诱导M1极化促进子痫前期免疫异常。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-06-15 DOI: 10.1093/biolre/ioaf061

Xixi Deng, Xueqi Li, Guiqiong Huang, Jiani Zhang, Tingting Xu, Ying Feng, Xiaodong Wang

{"title":"HMGB1 promotes immune abnormalities in preeclampsia by recruiting monocyte/decidual macrophages and inducing M1 polarization†.","authors":"Xixi Deng, Xueqi Li, Guiqiong Huang, Jiani Zhang, Tingting Xu, Ying Feng, Xiaodong Wang","doi":"10.1093/biolre/ioaf061","DOIUrl":"10.1093/biolre/ioaf061","url":null,"abstract":"Preeclampsia (PE) is a severe pregnancy complication, characterized by immune dysregulation and placental hypoxia. Decidua macrophages (dMφ) are essential at maternal-fetal interface, involves aberrant activation of dMφ toward the M1 phenotype in PE. High mobility group box 1 (HMGB1), released from necrotic cells after injury, accumulates in the placenta and peripheral blood of patients with PE. This study aims to investigate the interaction between macrophages and trophoblasts, exploring how HMGB1 affects macrophage functions and its potential involvement in the pathophysiology of PE. Decidua tissue was obtained from 37 women, comprising women with severe PE, PE, and normal pregnancies. HMGB1 levels in decidua were evaluated by immunohistochemistry, western blot, and quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR). Additionally, primary dMφ and peripheral blood monocytes (pMo) were isolated to develop a co-culture model simulating the maternal-fetal interface cell model of PE. Flow cytometric analysis and in vitro cell migration assay investigated the interaction between macrophages and HMGB1. This study identified elevated HMGB1 expression in PE patients, located in trophoblast, decidual stromal cells, and the extracellular matrix. Furthermore, hypoxia induced trophoblast to express and secrete HMGB1, promoting pMo and dMφ migration. Additionally, HMGB1 recruited monocyte-induced macrophages (pMφ) and dMφ, while driving M1 macrophage polarization. This study offers insights into the suppressive effects of the crosstalk between dMφ and trophoblasts at the maternal-fetal interface, lending credence to macrophage-targeted interventions of PE as a potential therapeutic strategy.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1273-1288"},"PeriodicalIF":3.1,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zinc eluted from glassware is a risk factor for embryo development in human and animal assisted reproduction†. 玻璃器皿中洗脱出的锌是人类和动物辅助生殖过程中胚胎发育的风险因素†。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-06-15 DOI: 10.1093/biolre/ioaf050

Tatsuma Yao, Hisato Kobayashi, Tatsuki Hirai, Yuta Tokuoka, Mikiko Tokoro, Yuta Asayama, Yuka Suzuki, Yu Hatano, Hiroki Ikeda, Satoshi Sugimura, Takuya Yamamoto, Takahiro G Yamada, Yoshihiko Hosoi, Akira Funahashi, Noritaka Fukunaga, Yoshimasa Asada, Kazuki Kurimoto, Kazuo Yamagata

{"title":"Zinc eluted from glassware is a risk factor for embryo development in human and animal assisted reproduction†.","authors":"Tatsuma Yao, Hisato Kobayashi, Tatsuki Hirai, Yuta Tokuoka, Mikiko Tokoro, Yuta Asayama, Yuka Suzuki, Yu Hatano, Hiroki Ikeda, Satoshi Sugimura, Takuya Yamamoto, Takahiro G Yamada, Yoshihiko Hosoi, Akira Funahashi, Noritaka Fukunaga, Yoshimasa Asada, Kazuki Kurimoto, Kazuo Yamagata","doi":"10.1093/biolre/ioaf050","DOIUrl":"10.1093/biolre/ioaf050","url":null,"abstract":"In assisted reproduction, many factors in the culture environment, including light, temperature, pH, and culture media, can reduce preimplantation embryo viability. Laboratory glassware is also a known risk factor for in vitro embryos; however, the underlying mechanisms that disrupt embryonic development remain unclear. We identified Zn eluted from glassware as an embryotoxic substance. In mouse embryos, Zn induced delayed development, abnormalities in chromosome segregation, cytokinesis, zygotic gene activation (e.g. Zscan4a and murine endogenous retrovirus with leucine, also known as MERVL), and aberrantly upregulated developmental gene expression (e.g. Hoxa1, Hoxb9, T, and Fgf8) that could be mediated through metal regulatory transcription factors (e.g. Mtf1). Subsequently, Zn exposure led to significantly reduced blastocyst formation. Post-implantation, Zn-exposed embryos were associated with normal birth rates, however, the birth weight increased by an average of 18% compared with embryos cultured without Zn. Furthermore, Zn exposure affected the development of bovine and human embryos, with species-based variation in the strength and timing of these effects. To mitigate these embryotoxic effects, we identified a method to prevent glass toxicity using chelating agents. This research not only highlights the importance of risk control in embryo culture but also facilitates the development of safe and effective methods for assisted reproduction.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1054-1071"},"PeriodicalIF":3.1,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ANXA1 inhibits trophoblast ferroptosis in preeclampsia by downregulating KISS1†. ANXA1通过下调KISS1抑制子痫前期滋养细胞铁下垂。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-06-15 DOI: 10.1093/biolre/ioaf060

Yuzhu Rao, Shiming Tan, Jingjing Wang, Jingqiu Jia, Zemin Cai, Chunyan Wu, Peng Wu, Zuo Wang

{"title":"ANXA1 inhibits trophoblast ferroptosis in preeclampsia by downregulating KISS1†.","authors":"Yuzhu Rao, Shiming Tan, Jingjing Wang, Jingqiu Jia, Zemin Cai, Chunyan Wu, Peng Wu, Zuo Wang","doi":"10.1093/biolre/ioaf060","DOIUrl":"10.1093/biolre/ioaf060","url":null,"abstract":"Preeclampsia (PE) is a serious hypertensive disorder of pregnancy, significantly affecting maternal and neonatal health worldwide. It remains a leading cause of morbidity and mortality. Recent studies suggest a potential link between ferroptosis and PE. Annexin A1 (ANXA1), an endogenous anti-inflammatory mediator, can be activated by glucocorticoids, ischemia-reperfusion, inflammation, or oxidative stress. Ac2-26, a synthetic peptide derived from the N-terminal 26 amino acids of ANXA1, retains its anti-inflammatory properties. However, the regulatory mechanisms of ANXA1 in PE are not fully understood. In this study, we first observed in creased ferroptosis and reduced ANXA1 expression in preeclamptic placentas. A PE-like mouse model further confirmed placental ferroptosis, which was ameliorated by Ac2-26 treatment, improving pregnancy outcomes. In vitro, Ac2-26 targeted ANXA1, alleviated RSL 3-induced trophoblast dysfunction, and inhibited lipid peroxidation. Mechanistically, we found that increased KISS1 expression is closely associated with ferroptosis and PE, and that ANXA1 (Ac2-26) suppresses KISS1 expression, thereby mitigating ferroptosis. In summary, our findings identify a novel mechanism in which elevated KISS1 promotes ferroptosis in PE, and this process is counteracted by ANXA1 (Ac2-26) via KISS1 downregulation. This discovery offers a promising therapeutic strategy targeting ferroptosis in PE.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1256-1272"},"PeriodicalIF":3.1,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heat stress during maturation of bovine oocytes profoundly impacts the mitochondrial bioenergetic profile and causes endoplasmic reticulum stress in subsequent blastocysts†. 牛卵母细胞成熟过程中的热应激对线粒体生物能量谱产生深远影响，并在随后的囊胚中引起内质网应激。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-06-15 DOI: 10.1093/biolre/ioaf070

Eva Held-Hoelker, Lientje Sophie Haake, Jessica Kurzella, Maibritt Schreiber, Christina Dauben, Dessie Salilew-Wondim, Nasser Ghanem, Franca Rings, Christine Große-Brinkhaus, Ernst Tholen, Dawit Tesfaye, Michael Hoelker

{"title":"Heat stress during maturation of bovine oocytes profoundly impacts the mitochondrial bioenergetic profile and causes endoplasmic reticulum stress in subsequent blastocysts†.","authors":"Eva Held-Hoelker, Lientje Sophie Haake, Jessica Kurzella, Maibritt Schreiber, Christina Dauben, Dessie Salilew-Wondim, Nasser Ghanem, Franca Rings, Christine Große-Brinkhaus, Ernst Tholen, Dawit Tesfaye, Michael Hoelker","doi":"10.1093/biolre/ioaf070","DOIUrl":"10.1093/biolre/ioaf070","url":null,"abstract":"In dairy cows, detrimental effects of global warming and intensive genetic selection for high milk yield on reproductive performance have become increasingly relevant in cooler regions. Based on the current knowledge, we hypothesized that elevated temperature during oocyte maturation affects mitochondria and endoplasmic reticulum in parallel with mitochondrial dysfunction, representing the mechanistic link between reactive oxygen species and endoplasmic reticulum stress. To prove that the present study aimed to uncover the consequences of heat stress during oocyte maturation on mitochondrial health, cellular oxidative stress response, and its implications for endoplasmic reticulum stress. Immature bovine oocytes were matured either under routine temperatures (38.8°C, Control) or exposed to elevated temperatures (41°C, heat stress). Metaphase II (MII) oocytes and subsequent blastocysts were analyzed in terms of developmental capacity, mitochondrial membrane potential, the bioenergetic profile, reactive oxygen species level, and expression of candidate genes playing a role in oxidative stress, endoplasmic reticulum stress, and apoptosis. While no effect on matured oocytes became obvious, heat stress embryos demonstrated typical alterations of the mitochondrial bioenergetic profile in terms of higher mitochondrial membrane potentials not going along with higher ATP-linked oxygen consumption, significantly lower maximum respiration, and spare capacity rates, implicating less efficient mitochondria accompanied with significantly higher reactive oxygen species levels. Moreover, gene expression of heat stress embryos supported the assumption that mitochondria are the mechanistic link between oxidative stress and endoplasmic reticulum stress, impairing early embryo development by promoting apoptosis.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1100-1113"},"PeriodicalIF":3.1,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0