Biology of Reproduction最新文献_第2页

Angiogenin-driven rRNA transcriptional activation regulates endometrial receptivity through epithelial remodeling†. 血管生成素驱动的rRNA转录激活通过上皮重塑调节子宫内膜容受性。

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-25 DOI: 10.1093/biolre/ioag084

Jiangxue Cai, Lu Lu, Meiling Tan, Chenxuan Li, Jianguo Zhu, Yiran Sun, Chenxi Liu, Hao Yang, Bin He

{"title":"Angiogenin-driven rRNA transcriptional activation regulates endometrial receptivity through epithelial remodeling†.","authors":"Jiangxue Cai, Lu Lu, Meiling Tan, Chenxuan Li, Jianguo Zhu, Yiran Sun, Chenxi Liu, Hao Yang, Bin He","doi":"10.1093/biolre/ioag084","DOIUrl":"https://doi.org/10.1093/biolre/ioag084","url":null,"abstract":"Endometrial receptivity is crucial for successful embryo implantation and the establishment of pregnancy. Although exogenous gonadotropin-controlled ovarian stimulation (OS) is widely used in assisted reproductive technology to induce multiple ovulations, it has been linked to impaired endometrial receptivity, and effective interventions remain limited. In this study, we demonstrate that OS disrupts endometrial receptivity in mice by interfering with epithelial remodeling, manifested as downregulation of tight junctions, aberrant epithelial proliferation, reduced adhesion, and abnormal epithelial morphology. Moreover, OS induced upregulation of angiogenin (Ang) in endometrial stromal cells, while its receptor was expressed in epithelial cells. Mechanistically, OS-elevated serum estradiol (E2) enhances Ang expression through ERα binding to the Ang promoter. In vitro, Ang disrupts the proliferation-differentiation balance in endometrial epithelial cells by enhancing rRNA transcription. Genetic deficiency of Ang attenuated OS-induced impairment of epithelial remodeling and restored normal epithelial architecture. Pharmacological inhibition of Ang with neomycin improved uterine receptivity, implantation rates, and fetal development in OS-treated mice. Collectively, our findings identify stromal-derived Ang as a critical mediator that regulates endometrial receptivity through activation of rRNA transcription. Targeting Ang signaling may represent a promising therapeutic strategy to improve endometrial receptivity and enhance in vitro fertilization outcomes.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular basis of chromosome segregation and age-associated errors in oocyte meiosis I. 卵母细胞减数分裂中染色体分离和年龄相关错误的分子基础。

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-25 DOI: 10.1093/biolre/ioag086

Tomoya S Kitajima

引用次数: 0

SPEM2 deficiency disrupts spermiation leading to oligoasthenoteratozoospermia and male infertility†. SPEM2缺乏会破坏精子，导致少精症和男性不育。

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-24 DOI: 10.1093/biolre/ioag087

Yong Li, Wenqing Lu, Lanlan Meng, Chen Tan, Caoling Xu, Tiantian Wang, Yuwei Yuan, Rui Yang, Chunbo Xie, Weili Wang, Chaofeng Tu, Wen-Bin He, Guang-Xiu Lu, Ge Lin, Liang Hu, Qian-Jun Zhang, Juan Du, Jianqiang Bao, Yue-Qiu Tan

{"title":"SPEM2 deficiency disrupts spermiation leading to oligoasthenoteratozoospermia and male infertility†.","authors":"Yong Li, Wenqing Lu, Lanlan Meng, Chen Tan, Caoling Xu, Tiantian Wang, Yuwei Yuan, Rui Yang, Chunbo Xie, Weili Wang, Chaofeng Tu, Wen-Bin He, Guang-Xiu Lu, Ge Lin, Liang Hu, Qian-Jun Zhang, Juan Du, Jianqiang Bao, Yue-Qiu Tan","doi":"10.1093/biolre/ioag087","DOIUrl":"https://doi.org/10.1093/biolre/ioag087","url":null,"abstract":"Oligoasthenoteratozoospermia (OAT) is a prevalent phenotype among infertile males, yet its underlying genetic etiology remains largely undefined. In this study, we found Spem2 deficiency did not affect the development of spermatogenic cells or the eventual generation of elongated spermatids within the testis, but affected the normal formation of residual bodies during the late stage of spermiation, which led to incomplete cytoplasmic removal of spermatids, abnormal sperm release and caused OAT. Affected sperm exhibited severely bent heads, cytoplasmic remnants encasing the head, and multiple sperm gathered. We further found SPEM2 may be involved in maintaining spermiation and cell polarity through interactions with cell polar molecules VANGL2, PRICKLE3 and DVL3. In vitro experiments showed that the transmembrane region of SPEM2 was its key functional domain and the binding domain for interactions between SPEM2 and VANGL2. Importantly, Spem2-deficient sperm enabled successful fertilization via ICSI. Three novel SPEM2 heterozygous pathogenic variants were identified in four OAT patients by the whole-exome sequencing. Our findings revealed key roles of SPEM2 in spermiation, explored the potential pathogenesis of male infertility caused by SPEM2 deficiency, and provided essential information for genetic and reproductive counseling for such patients.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interleukin-6 and Leukemia Inhibitory Factor Differentially Modify Embryonic Disk Composition in Post Hatching Bovine Embryos†. 白细胞介素-6和白血病抑制因子对孵化后牛胚胎盘组成的影响

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-21 DOI: 10.1093/biolre/ioag083

Mary A Oliver, Vitor R G Mercadante, Alan D Ealy

{"title":"Interleukin-6 and Leukemia Inhibitory Factor Differentially Modify Embryonic Disk Composition in Post Hatching Bovine Embryos†.","authors":"Mary A Oliver, Vitor R G Mercadante, Alan D Ealy","doi":"10.1093/biolre/ioag083","DOIUrl":"https://doi.org/10.1093/biolre/ioag083","url":null,"abstract":"Proper formation of the embryonic disk is essential for pregnancy survival. This study examined whether supplementing interleukin-6 (IL6) or leukemia inhibitory factor (LIF) influence epiblast (EPI) and hypoblast (HYPO) development as well as other facets of post-hatching bovine embryo development in an extended culture system. A series of three studies were completed where in vitro-produced (IVP) embryos were treated with IL6 or LIF from days 5-12, 5-7, or 7-12 post-fertilization. Survival and hatching were monitored from days 7-12, then embryos were fixed for immunofluorescent assessments. Non-treated IVP embryos were transferred at day 7 and collected on day 12 post-estrus (IVP-ET) for use an in-utero reference for the days 7-12 culture group. Neither IL6 nor LIF affected survival or hatching. Supplementing IL6 from days 5-12 increased EPI cell number and reduced HYPO formation whereas LIF had no effect. Supplementing IL6 from days 7-12 produced similar responses, and EPI cell numbers matched those of the IVP-ET day 12 embryos. By contrast, supplementing IL6 from days 5-7 had no effects, indicating that IL6 exposure is unnecessary before blastocyst formation but required afterward to elicit these outcomes. In conclusion, IL6 supplementation after blastocyst formation enhances EPI development to levels comparable to in utero embryos, although this may occur at the expense of HYPO formation. It was equally intriguing that LIF did not mimic the effects of IL6 but rather failed to exhibit any noteworthy effects on the EPI and HYPO lineages.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147728161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a germline cell-containing ovarian cell line capable of oocyte generation in vitro from sterlet Acipenser ruthenus†. 含种系细胞的小鲟体外能产生卵母细胞卵巢细胞系的建立。

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-20 DOI: 10.1093/biolre/ioag076

Ai Sun, Hua Zhu, Tian Dong, Zhaohui Tian, Hailiang Song, Hongxia Hu

{"title":"Establishment of a germline cell-containing ovarian cell line capable of oocyte generation in vitro from sterlet Acipenser ruthenus†.","authors":"Ai Sun, Hua Zhu, Tian Dong, Zhaohui Tian, Hailiang Song, Hongxia Hu","doi":"10.1093/biolre/ioag076","DOIUrl":"https://doi.org/10.1093/biolre/ioag076","url":null,"abstract":"Sturgeons (order Acipenseriformes) represent one of the most ancient lineages of Actinopterygii. Presently, most sturgeon species are classified as critically endangered. In this study, a novel ovarian cell line which contained germline cells derived from the sterlet (Acipenser ruthenus) was established, designated as the ARO cell line. Germline cells in the cell line were identified using marker gene analysis, alkaline phosphatase activity assay and immunofluorescence staining. Transmission electron microscope observation showed that previtellogenic oocytes existed in the ARO cell line. Flow cytometry analysis indicated the cell line maintained meiotic competence to generate DNA reduced ploidy (diploid) oocytes. Under 3D culture condition, germinal vesicle (GV) stage, germinal vesicle breakdown (GVBD) stage and meiosis I oocytes were found. Furthermore, the cell line exhibited sustained proliferation and self-organization into reconstituted ovaries (rOvaries). Transcriptome analysis of rOvaries revealed that rOvaries had formed cell junctions and normal intercellular signaling, and possessed normal endocrine function and oocyte-producing capacity. In summary, the established ARO cell line exhibited stable passaging over multiple generations in vitro, was capable of sustainably generating oocytes, and was expected to be applicable to the establishment of ovarian organoids.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoscale 3D Anatomy of the Western Flower Thrips Reproductive System Revealed by Volume Electron Microscopy: Extreme Miniaturization and Evolutionary Adaptations for Pest Fecundity. 体积电子显微镜显示的西花蓟马生殖系统的纳米级三维解剖：极端小型化和进化适应害虫繁殖力。

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-17 DOI: 10.1093/biolre/ioag079

Guan Wang, Jian-Sheng Guo, Dan-Ting Li, Zeng-Rong Zhu, Chuan-Xi Zhang

{"title":"Nanoscale 3D Anatomy of the Western Flower Thrips Reproductive System Revealed by Volume Electron Microscopy: Extreme Miniaturization and Evolutionary Adaptations for Pest Fecundity.","authors":"Guan Wang, Jian-Sheng Guo, Dan-Ting Li, Zeng-Rong Zhu, Chuan-Xi Zhang","doi":"10.1093/biolre/ioag079","DOIUrl":"https://doi.org/10.1093/biolre/ioag079","url":null,"abstract":"Frankliniella occidentalis (western flower thrips, WFT) is a globally destructive pest causing major crop losses via oviposition, feeding, and plant virus transmission. Previous work has focused on its reproductive physiology and behavior, but comprehensive 3D structural knowledge remains limited. We used volume electron microscopy (vEM) to achieve nanoscale 3D visualization of the reproductive systems in both sexes, providing the first high-resolution, in situ reconstruction and establishing a new anatomical framework for insect reproductive physiology. Males exhibit extreme miniaturization, with reproductive organs occupying just 1.45 % of body volume yet retaining full function within a compact abdomen. Females possess eight tightly interwoven ovarioles that maximize coelomic space for oocyte maturation, maintaining a slender form while housing large eggs-reflecting an evolutionary trade-off between reproductive efficiency and body size. Our reconstructions resolve a key ambiguity: the female accessory glands are a pair of fused, S-shaped structures joined by an internal duct opening near the ovipositor base. Quantitative analysis reveals the first complete germ cell census, with asymmetric ovary cell numbers suggesting an adaptive strategy for sustained fecundity. By integrating detailed morphology with systematic quantification, this study creates a foundation for research in functional morphology and for developing reproduction-targeted pest management. The structural insights enable precise strategies to disrupt thrips reproduction and reduce virus spread. Beyond advancing pest control innovation, the findings serve as a valuable educational and reference resource in insect morphology.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147697456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of endocrine-disrupting chemicals on uterine diseases. 内分泌干扰物对子宫疾病的影响。

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-17 DOI: 10.1093/biolre/ioag082

Kanako Hayashi, James A MacLean

引用次数: 0

Strain- and lineage-specific regulation of lipid droplet metabolism in the mouse blastocyst†. 小鼠囊胚脂滴代谢的品系和谱系特异性调控

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-16 DOI: 10.1093/biolre/ioag080

Dominika Żbikowska, Abdul Majid Khan, Marta Marlena Ziętek, Domenico Iuso, Silvestre Sampino

{"title":"Strain- and lineage-specific regulation of lipid droplet metabolism in the mouse blastocyst†.","authors":"Dominika Żbikowska, Abdul Majid Khan, Marta Marlena Ziętek, Domenico Iuso, Silvestre Sampino","doi":"10.1093/biolre/ioag080","DOIUrl":"https://doi.org/10.1093/biolre/ioag080","url":null,"abstract":"Lipid droplets (LDs) are multifunctional organelles that serve as reservoirs of fatty acids, which act as substrates for β-oxidation and ATP synthesis during mammalian preimplantation development. However, the genetic and lineage-specific regulation of their biogenesis and utilization remains poorly understood. Here, we report a mouse model of embryonic LD deficiency, the BTBR T+ Itpr3tf/J inbred strain, characterized by the absence of oocyte-inherited LD clusters in zygotes and a rapid depletion of de novo generated LDs at the morula-to-blastocyst transition stage, as compared to C57BL/6J controls. Lineage-resolved analyses revealed a selective depletion of large LDs in the trophectoderm while lipids were consistently accumulated in the inner cell mass, indicating lineage-specific lipid utilization. Pharmacological inhibition of autophagy or cytoplasmic lipase activity caused LD accumulation and developmental arrest specifically in BTBR, indicating an essential dependence on lipid mobilization. Modulation of mitochondrial fatty acid β-oxidation by etomoxir inhibition and L-carnitine stimulation shifted lipid droplet enlargement and allocation between lineages in a strain-specific manner, and altered epiblast cell numbers, linking lipid catabolism to lineage fitness. These findings identify a genetically encoded, lineage-specific LD program linking lipid mobilization to blastocyst morphogenesis, embryonic survival, and lineage homeostasis.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

cAMP signaling in the placenta: a pivotal determinant of trophoblast fate and pregnancy outcomes. 胎盘中的cAMP信号：滋养细胞命运和妊娠结局的关键决定因素。

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-16 DOI: 10.1093/biolre/ioag081

Enoch Appiah Adu-Gyamfi, Daniel Neubauer

{"title":"cAMP signaling in the placenta: a pivotal determinant of trophoblast fate and pregnancy outcomes.","authors":"Enoch Appiah Adu-Gyamfi, Daniel Neubauer","doi":"10.1093/biolre/ioag081","DOIUrl":"https://doi.org/10.1093/biolre/ioag081","url":null,"abstract":"Cyclic adenosine monophosphate (cAMP) signaling is a crucial pathway which regulates a myriad of physiological processes. cAMP is synthesized by adenylyl cyclases and then degraded by phosphodiesterases (PDEs). This intricate interplay of synthesis and degradation, often localized to specific subcellular compartments, tightly regulates cAMP signaling in a context-dependent manner, ensuring that the normal levels of cAMP needed for specific cellular processes are maintained within cells. The downstream actions of cAMP are mediated by effectors such as protein kinase A (PKA) and exchange proteins activated by cAMP (EPAC). At the feto-maternal interface, these actions ensure a proper timing and spatial regulation of trophoblast proliferation, survival and differentiation along the villous and extravillous pathways, leading to successful placental development and pregnancy progression. Disruptions in cAMP signaling result in mal-formed and dysfunctional placentas, thus causing pregnancy complications such as preeclampsia and fetal growth restriction. This suggests that cAMP modulation in the uterine environment right after embryo implantation is a potential therapeutic strategy to prevent abnormal placentation and the associated pregnancy disorders.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Derivation of primed sheep embryonic stem cells and conversion to an intermediate naïve-like state. 引物羊胚胎干细胞的衍生和转化到中间naïve-like状态。

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-16 DOI: 10.1093/biolre/ioag078

T S Shyamkumar, Manuel A Vasquez-Hidalgo, Viju V Pillai

{"title":"Derivation of primed sheep embryonic stem cells and conversion to an intermediate naïve-like state.","authors":"T S Shyamkumar, Manuel A Vasquez-Hidalgo, Viju V Pillai","doi":"10.1093/biolre/ioag078","DOIUrl":"https://doi.org/10.1093/biolre/ioag078","url":null,"abstract":"Embryonic stem cells (ESCs) derived from the inner cell mass of embryos possess unlimited self-renewal and pluripotency, offering a powerful system to study early development and enable genetic and biotechnological innovation. Although several livestock ESC lines have been reported in recent years, defining culture conditions that support stable long-term self-renewal and controlled transitions across pluripotent states remains challenging. Here, we report the de novo derivation of sheep (ovine) embryonic stem cells (oESCs) from in vivo blastocysts using a chemically defined culture system. The derived cells exhibit morphological and molecular features of primed pluripotency and can be propagated under both feeder-dependent and feeder-free conditions without loss of identity or karyotypic stability. Building on this foundation, we developed enhancer-driven reporter lines that faithfully reflect (Octamer-binding transcription factor 4) OCT4 and (SRY-box transcription factor 2) SOX2 transcriptional activity, enabling dynamic visualization of pluripotency and differentiation in live cultures. These reporter systems revealed the responsiveness of oESCs to signaling modulation and provided a functional readout of pluripotency state transitions. When cultured in defined media previously shown to stabilize naïve pluripotency in human ESCs, oESCs adopted dome-shaped colony morphology, maintained OCT4, SOX2, and NANOG expression, retained differentiation potential, and exhibited a transcriptomic profile consistent with resetting to an intermediate pluripotent state with naïve-like morphological features. These findings establish stable ovine ESC lines and demonstrate their plasticity across the pluripotency spectrum, providing a valuable platform for investigating ruminant stem cell biology and advancing livestock biotechnology.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0