Biology of Reproduction最新文献

{"title":"Testing the function of PPP-family phosphatases modified by luteinizing hormone signaling in mouse ovarian follicles: an update.","authors":"Jeremy R Egbert, Corie M Owen, Tracy F Uliasz, Deborah Kaback, Siu-Pok Yee, Laurinda A Jaffe","doi":"10.1093/biolre/ioaf076","DOIUrl":"https://doi.org/10.1093/biolre/ioaf076","url":null,"abstract":"","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA Splicing and Alternative Polyadenylation Profile during Sheep Zygotic Genome Activation.","authors":"Mingtian Deng, Hua Yang, Ying Chen, Nasser Ghanem, Yingnan Yang, Xiaowei Chen, Zhang JinHao, Feng Wang, Liqin Wang, Yanli Zhang","doi":"10.1093/biolre/ioaf068","DOIUrl":"https://doi.org/10.1093/biolre/ioaf068","url":null,"abstract":"<p><p>Zygotic genome activation (ZGA) is a critical biological step in mammalian early embryo development. However, ZGA initiation in sheep and the related sophisticated RNA metabolism remains largely unknown. Here, we observed extensive alterations in gene expression and DNA methylation patterns, along with elevated levels of RNA polymerase II (RNAPII) and its phosphorylation at serine 2 (RNAPII-Ser2P) at the 16-cell stage. Moreover, the embryos were blocked at the 16-cell stage embryo when treated with α-Amanitin, indicating that ZGA is initiated at the 16-cell stage in sheep in vitro fertilized embryos. To uncover the sophisticated RNA metabolism during ZGA, we conducted weighted gene co-expression network analysis and identified 1957 critical maternal genes, including TET3, UHRF1 and KIF2C. Using dapars analysis, we discovered 1058 and 933 lengthened alternative polyadenylation (APA) events during ZGA in sheep and mice. Specifically, genes exhibiting shorten APA were highly expressed at sheep 16-cell stage embryos and mouse 2-cell stage embryos. During ZGA in sheep and mice, 2675 and 1963 genes showed exon skipping, an alternative splicing (AS) events, which is related to RNA binding, translation, gamete generation, and reproduction. Of note, inhibition of AS led to 2-cell block in mice and 8/16-cell block in sheep. Moreover, 5-EU, RNAPII, and RNAPII-Ser2P signal were decreased in AS inhibited 2-cell embryos in mice, suggesting AS might regulate the ZGA process by crosstalk with RNAPII. In conclusion, our data confirmed ZGA initiation at the 16-cell stage embryos, and provides insights into the complex RNA metabolism during ZGA in mammals.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of cholesterol/ALKBH1/ITGA2B in thrombosis in the placental vessels of preeclampsia.","authors":"Yingying Zhang, Eryun Zhang, Ling Li, Yongwei Ren, Jinqiu Zhang, Qiutong Zheng, Minya Sun, Shaojie Zhao, Yugui Cui, Ying Gu, Zhice Xu","doi":"10.1093/biolre/ioaf067","DOIUrl":"https://doi.org/10.1093/biolre/ioaf067","url":null,"abstract":"<p><p>The risk of multiple placental thrombosis is significantly elevated in preeclampsia (PE), and the vascular endothelium plays a vital role in coagulation processes through the secretion of ITGA2B, vWF, and TF. However, the underlying pathological mechanisms remain unclear. In this study, placental blood vessels were collected from both PE and normal pregnancies. Protein levels of ITGA2B were found to be up-regulated in PE samples compared to controls, indicating enhanced coagulation function in PE placental tissue. Additionally, Higher cholesterol levels of plasma and lower ALKBH1 expression in placental blood vessels of PE were found, overall DNA N6-methyldeoxyadenosine (6 mA) levels were up-regulated. Interestingly, the DNA 6 mA level at the ITGA2B promoter was decreased. Cholesterol overload experiments in human umbilical vein endothelial cells (HUVECs) demonstrated that cholesterol regulates the protein expression of ALKBH1 and ITGA2B in a concentration-dependent manner. Furthermore, ITGA2B protein expression was increased following ALKBH1 knockout, but no changes were observed when cholesterol was incubated with ALKBH1 knockout cells. These findings provide critical insights into the roles of cholesterol levels, ALKBH1, and ITGA2B in coagulation function within placental blood vessels. This information may contribute to further investigations of potential therapeutic targets and early prevention strategies for placental thrombosis in PE.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role(s) of HE4 and other WAP factors for sperm functions and male urogenital diseases.","authors":"Xiaolun Xu, Huaiyun Tang, Shuai Zhang, Lingjin Tuo, Lisha Tang, Shi-Wen Jiang","doi":"10.1093/biolre/ioaf063","DOIUrl":"https://doi.org/10.1093/biolre/ioaf063","url":null,"abstract":"<p><p>HE4 (human epididymis protein 4) is a secretory glycoprotein with protease inhibitor activity. HE4 is constitutively expressed in the epididymis, testis, prostate, and other male urogenital organs. By its overexpression in the lesion and serum of cancer patients, HE4 is used as a biomarker for patient triage prognosis and recurrent surveillance of gynecologic malignancies. Elevated seminal plasma levels of HE4 have been correlated to oligoasthenozoospermia, and genetic overexpression of HE4 gene in mice led to the development of small testis, Leydig cell hyperplasia, structural anomalies of seminiferous tubules, elevated serum testosterone level, and decreased male fecundity. Since proteases and their endogenous inhibitors are deeply involved in semen liquefaction and sperm maturation/capacitation, motility, acrosomal reaction and fertilization, HE4 and other WAP domain protease inhibitors may participate in the regulation of various sperm functions and male fertility. In addition, increasing data have implicated HE4 and other WAP domain factors such as SLPI in innate immunity and inflammation, and these factors may play a role(s) in male urogenital inflammatory disorders such as epididymitis, testitis and prostatitis. This review summarizes relevant observations from in vitro, in vivo and clinical studies, and analyzes the potential role(s) of HE4 in male infertility and other urogenital diseases. Critical issues concerning HE4 physiopathological functions, mechanisms as well as the current/future research efforts, are raised and discussed.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The roles of AKT isoforms in decidualization and embryo implantation using a PGR-Cre mouse model.","authors":"Pascal Adam, François Fabi, Sophie Parent, Léa-Isabelle Renaud, Monique Cadrin, Eric Asselin","doi":"10.1093/biolre/ioaf062","DOIUrl":"https://doi.org/10.1093/biolre/ioaf062","url":null,"abstract":"<p><p>Implantation is a complex process requiring a prepared, receptive endometrium, reliant on synchronized decidualization of stromal cells. During this process, cell proliferation and apoptosis are tightly regulated by signaling factors, including the survival and proliferation PI3K/AKT pathway. The three AKT isoforms each play distinct physiological roles but their specific functions in endometrial cell survival and apoptosis remain unclear. We hypothesize that for successful implantation, each AKT isoform has distinct roles in the endometrium during decidualization, which varies throughout the process. To explore this, we developed a unique PGR-Cre tissue-specific mouse model with single and combined knockouts (KO) of each AKT isoform. Using artificial decidualization during pseudopregnancy and normal gestation, we investigated the specific activity of each AKT isoform and their downstream targets to assess the role of AKT pathway. Our results showed that the AKT1-2 KO genotype failed to decidualize during pseudopregnancy and exhibited a reduced number of implantation sites. Interestingly, AKT3 was hyperphosphorylated in the AKT1-2 KO mice and emerged as the primary isoform active throughout decidualization, specifically signaling through GSK3B. This study suggests distinct yet partially redundant roles for AKT1 and AKT2 during decidualization and embryo implantation. We propose that the AKT pathway plays significant role in fertility, and a deeper understanding of its involvement in decidualization could lead to improved strategies for addressing fertility issues. These findings highlight the importance of AKT activity in the cellular and molecular regulation of mouse fertility.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic targeting of the tryptophan-kynurenine Axis for HTR-8/SVneo trophoblast proliferation and migration in unexplained recurrent spontaneous abortion.","authors":"Pingping Jin, Xinyi Lu, Lu Wang, Yan Chen, Lan Yang, Yongxiang Yin, Ye Shen, Xinxin Ni, Daozhen Chen, Yun Zhang, Yu Chen","doi":"10.1093/biolre/ioaf040","DOIUrl":"https://doi.org/10.1093/biolre/ioaf040","url":null,"abstract":"<p><strong>Introduction: </strong>Recurrent spontaneous abortion (RSA) is associated with maternal-fetal interface dysfunction, particularly abnormal trophoblast invasion and proliferation. However, our understanding of the cause of RSA remains limited.</p><p><strong>Methods: </strong>Plasma Trp and Kyn levels were measured in two groups using ELISA. Immunofluorescence and western blot analyses were employed to evaluate the expression of IDO1, VEGFA, and proteins associated with epithelial-mesenchymal transition (EMT) in villous and decidual tissues from patients with recurrent spontaneous abortion (RSA). The effects of Tryptophan (Trp) and IDO1-driven Trp-Kynurenine (Kyn) metabolism on trophoblast proliferation, migration, EMT, and angiogenesis were investigated in the HTR-8/SVneo cell line using wound healing, transwell migration, quantitative real-time PCR (RT-qPCR), Western blotting, and tube formation assays. RNA sequencing identified differentially expressed genes in cells treated with 500 μM exogenous L-Trp.</p><p><strong>Results: </strong>RSA patients exhibited elevated plasma Trp levels and significantly reduced Kyn levels, indicating decreased IDO1 activity (as assessed by the Kyn/Trp ratio) compared to controls. IDO1, EMT-related proteins, and VEGFA were downregulated in RSA patient tissues. In vitro, L-Trp enhanced trophoblast migration, invasion, EMT, and microvasculature formation via IDO1 activation. The reduced functional capabilities induced by the IDO1 antagonist 1-MT (500 μM) were rescued by Kyn (300 μM). RNA sequencing revealed that L-Trp upregulation modulates trophoblast gene expression and functional pathways associated with amino acid metabolism, angiogenesis, and vasculature development.</p><p><strong>Discussion: </strong>Our study reveals a novel molecular mechanism by which Trp metabolism regulates HTR-8 cell function, suggesting that modulating IDO1 activity may represent a therapeutic strategy to improve trophoblast function and pregnancy outcomes in RSA.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HMGB1 promotes immune abnormalities in preeclampsia by recruiting monocyte/decidual macrophages and inducing M1 polarization.","authors":"Xixi Deng, Xueqi Li, Guiqiong Huang, Jiani Zhang, Tingting Xu, Ying Feng, Xiaodong Wang","doi":"10.1093/biolre/ioaf061","DOIUrl":"https://doi.org/10.1093/biolre/ioaf061","url":null,"abstract":"<p><p>Preeclampsia (PE) is a severe pregnancy complication associated with immune imbalance and placental hypoxia stress. Decidua macrophages (dMφ) are essential at maternal fetal interface, which is abnormally activated and excessively transformed to the M1-phenotype in PE. High mobility group box 1(HMGB1), released from necrotic cells after injury, accumulates in the placenta and peripheral blood of patients with PE. Therefore, this study aims to investigate the interaction between macrophages and trophoblasts, exploring how HMGB1 affects macrophage functions and its potential involvement in the pathophysiology of PE. Decidua tissue was obtained from 11 women with severe pre-eclampsia (sPE), 13 women with pre-eclampsia (PE), and 13 women with normal pregnancies. HMGB1 levels in decidua were evaluated by immunohistochemistry (IHC), western blot, and qPCR. Additionally, primary dMφ and peripheral blood monocytes (pMo) were isolated to develop a co-culture model simulating maternal fetal interface cell model of PE. Flow cytometric analysis and in vitro cell migration assay investigated the interaction between macrophages and HMGB1. This study identified elevated HMGB expression in PE patients. HMGB1 expressed widely in trophoblast, decidual stromal cells, and the extracellular matrix. Furthermore, hypoxia induced trophoblast to express and secrete HMGB1, promoting pMo and dMφ migration. Additionally, HMGB1 recruited monocyte-induced macrophages (pMφ) and modulated M1 macrophage polarization. HMGB1 is increased at the maternal-fetal interface of PE, which recruits monocytes and macrophages and induces their polarization to M1. This study offers insights into the suppressive effects of the crosstalk between dMφ and trophoblasts at the maternal-fetal interface, lending credence to macrophage-targeted interventions of PE as a potential therapeutic strategy.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANXA1 Inhibits Trophoblast Ferroptosis in Preeclampsia by Downregulating KISS1.","authors":"Yuzhu Rao, Shiming Tan, Jingjing Wang, Jingqiu Jia, Zemin Cai, Chunyan Wu, Peng Wu, Zuo Wang","doi":"10.1093/biolre/ioaf060","DOIUrl":"https://doi.org/10.1093/biolre/ioaf060","url":null,"abstract":"<p><p>Preeclampsia (PE) is a significant hypertensive disorder associated with pregnancy, impacting the health of women and children globally. It stands as one of the primary contributors to elevated morbidity and mortality rates among pregnant individuals and neonates. Recent investigations indicate a significant potential association between ferroptosis and PE. Annexin A1 (ANXA1) serves as an endogenous inhibitor of inflammation, capable of being activated by glucocorticoids, ischemia-reperfusion events, inflammatory processes, or oxidative stress. Ac2-26 is a synthetic peptide derived from the N-terminal 26 amino acids of the ANXA1 protein and retains its anti-inflammatory properties. Nevertheless, the precise regulatory mechanisms underlying ANXA1's role in PE remain to be fully elucidated. In this study, we first revealed that the increase in ferroptosis in preeclamptic placentas is accompanied by a downregulation of ANXA1 expression. Next, we established a PE-like mouse model and confirmed the presence of ferroptosis in the placentas of these mice. Ac2-26 treatment reduced placental ferroptosis and improved adverse pregnancy outcomes. Additionally, we demonstrated that targeting ANXA1 (Ac2-26) alleviates RSL3-induced trophoblast dysfunction and inhibits its promotion of intracellular lipid peroxidation. Subsequent mechanistic investigations have demonstrated that the elevation of KISS1 levels is intricately associated with ferroptosis and PE, while ANXA1 (Ac2-26) serves to inhibit KISS1 expression, thereby ultimately mitigating ferroptosis. In summary, this study presents the novel finding that elevated levels of KISS1 during pregnancy promote ferroptosis, a process that is mitigated by ANXA1 (Ac2-26) through the downregulation of KISS1 expression, thereby alleviating PE. This discovery offers a promising therapeutic strategy targeting the ferroptosis signaling pathway in PE.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental Programming: Mechanisms of Early Exposure to Real-Life Chemicals in Biosolids on Offspring Ovarian Dynamics†.","authors":"Yiran Zhou, Katherine M Halloran, Michelle Bellingham, Richard G Lea, Neil P Evans, Kevin D Sinclair, Peter Smith, Vasantha Padmanabhan","doi":"10.1093/biolre/ioaf053","DOIUrl":"https://doi.org/10.1093/biolre/ioaf053","url":null,"abstract":"<p><p>Female reproductive capacity is shaped by ovarian reserve and patterns of follicle development. Ovarian reserve depletion occurs by follicle activation and atresia, which can be affected by environmental chemicals (ECs). Because humans are simultaneously exposed to hundreds of ECs, real-life exposure models are essential to assess patterns of atresia after EC exposure. Previous findings demonstrate maternal preconceptional and gestational ECs exposure via biosolids-treated pasture (BTP) increases activation rate and reduces primordial follicle pool in juveniles, but not adults. We hypothesized that this shift involves changes in death and proliferative pathways that impact follicle atresia from juvenile to adult life. Ovaries were collected from juvenile (9.5 weeks) and adult (2.5 years) offspring from ewes grazed on either BTP or inorganic fertilizer-treated pasture (Control). Follicular atresia was assessed through morphological characteristics and molecular death pathways, including expression of markers for apoptosis (CASP3), autophagy (LC3), and ferroptosis (GPX4) and proliferation (Ki67). There were higher levels of apoptosis and autophagy, and lower proliferation, in juvenile BTP offspring compared to controls. In adult BTP offspring, apoptosis and proliferation did not differ, autophagy was lower, and ferroptosis was higher compared to controls. Apoptosis was lower and ferroptosis higher in adults than in juveniles, regardless of treatment. Adult BTP offspring had lower autophagy and similar proliferation levels to juvenile BTP. These findings suggest that lower autophagy and lack of decrease in proliferation contribute to normalization of activation rate and ovarian pool in BTP adults and supportive of lasting impacts of gestational EC exposure on offspring follicular health.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishing the baseline: understanding follicle activation morphometrics in multiple mammalian species.","authors":"Hana Kubo, Christopher J McCauley, Mary B Zelinski, Monica M Laronda","doi":"10.1093/biolre/ioaf059","DOIUrl":"https://doi.org/10.1093/biolre/ioaf059","url":null,"abstract":"<p><p>Folliculogenesis encompasses many stages as the somatic granulosa and theca cells support oocytes through growth and maturation. A novel follicle stage, between primordial and transitional stages, was identified in mice and defined as \"zip\". Like all other follicle stages, the \"zip\" stage is characterized by its granulosa cell morphology. The \"wedge\" GC morphology in zip follicles is predicted to be the first granulosa cell division, marking the transition from squamous to cuboidal morphology. Here, zip and transitional stages were identified in histological sections of porcine, bovine, rhesus monkey, and human ovaries. Several growth dynamics characterized at these follicle stages were conserved between species. Oocyte diameter and area increased between the primordial and transitional stages in the porcine ovary and between the primordial and primary stages in the rhesus monkey ovary but appeared unchanged in bovine and human ovaries. In all species except for pigs, granulosa cell number and height increased at stages earlier than observed changes in the oocyte. Furthermore, there were differences in the percentage of zip and transitional follicle stages present in the cortical region across species. This implies that there may be species-dependent activation and growth mechanisms that require further study. The parameters defined here for identifying and characterizing the zip and transitional follicle stages across species can act as a tool for measuring factors that perturb or induce primordial follicle activation or effect follicle morphometric parameters in support of future innovations for fertility preservation and restoration.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}