The effects of sika deer oviduct epithelial cell-derived extracellular vesicles on oocytes and parthenogenetic embryos.

IF 3.1 2区 生物学 Q2 REPRODUCTIVE BIOLOGY
Bing Hu, Shu-Ming Shi, Xian-Feng Yu, Yu-Yan He, Zhi-Chao Chi, Lai-Ming Tian, Guan-Lin Jia, Ilkeun Kong, Yong-Xun Jin, Ming-Jun Zhang
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Abstract

Oviducts contain various nutrients that provide energy during oocyte development. This study aimed to improve the efficiency of in vitro reproduction using extracellular vesicles (EVs) produced by the oviduct epithelial cells of sika deer (Cervus nippon). Surprisingly, the uptake of deer oviduct epithelial cell extracellular vesicles (DOEC-EVs) by cumulus-oocyte complexes, which were encapsulated by dense cumulus cells (CCs), occurred only in CCs during maturation. Therefore, we hypothesized that DOEC-EVs are transported to oocytes through CCs to exert their effects. We first investigated the effects of DOEC-EVs on the expansion capacity of the cumulus-oocyte complexes, as well as cell cycle progression, proliferation, apoptosis, and lactate and pyruvate levels in CCs, and examined reactive oxygen species levels, mitochondrial function, and key gene expression. The results showed that DOEC-EVs regulated cell cycle progression, promoted proliferation, reduced apoptosis, and improved antioxidant capacity and glycolysis, and through the oocyte first polar body excretion rate, reactive oxygen levels and mitochondrial membrane potential, it was shown that CC promoted in vitro oocyte maturation, improved the antioxidant capacity and mitochondrial function of oocytes, and promoted parthenogenetic embryo development. These results suggest that DOEC-EVs improve the efficiency of oocyte development in deer in vitro by acting on CCs, laying the foundation for further research on in vitro deer reproduction.

梅花鹿输卵管上皮细胞来源的细胞外囊泡对卵母细胞和孤雌胚胎的影响。
输卵管含有各种营养物质,在卵母细胞发育过程中提供能量。本研究旨在提高梅花鹿输卵管上皮细胞产生的细胞外囊泡(EVs)的体外繁殖效率。令人惊讶的是,鹿输卵管上皮细胞胞外囊泡(doec - ev)被致密积云细胞(CCs)包裹的卵母细胞复合物摄取,只发生在成熟过程中的CCs中。因此,我们假设doec - ev通过cc转运到卵母细胞中发挥作用。我们首先研究了doec - ev对卵母细胞复合物扩张能力、细胞周期进程、增殖、凋亡、乳酸和丙酮酸水平的影响,并检测了活性氧水平、线粒体功能和关键基因表达。结果表明,doec - ev可调节细胞周期进程,促进细胞增殖,减少细胞凋亡,提高抗氧化能力和糖酵解能力,并通过卵母细胞第一极体排泄率、活性氧水平和线粒体膜电位表明,CC可促进体外卵母细胞成熟,提高卵母细胞抗氧化能力和线粒体功能,促进孤雌胚胎发育。上述结果表明,doec - ev通过作用于cc提高了鹿体外卵母细胞发育效率,为进一步研究鹿体外繁殖奠定了基础。
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来源期刊
Biology of Reproduction
Biology of Reproduction 生物-生殖生物学
CiteScore
6.30
自引率
5.60%
发文量
214
审稿时长
1 months
期刊介绍: Biology of Reproduction (BOR) is the official journal of the Society for the Study of Reproduction and publishes original research on a broad range of topics in the field of reproductive biology, as well as reviews on topics of current importance or controversy. BOR is consistently one of the most highly cited journals publishing original research in the field of reproductive biology.
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