Biology of Reproduction最新文献_第10页

Coiled-coil domain containing 159 is required for spermatid head and tail assembly in mice†. 小鼠精子头部和尾部的组装需要含有 159（CCDC159）的盘绕线圈结构域。

IF 3.6 2区生物学

Biology of Reproduction Pub Date : 2024-05-09 DOI: 10.1093/biolre/ioae012

Tingting Ge, Lu Yuan, Linwei Xu, Fan Yang, Wenhua Xu, Changmin Niu, Guanghua Li, Huiping Zhou, Ying Zheng

{"title":"Coiled-coil domain containing 159 is required for spermatid head and tail assembly in mice†.","authors":"Tingting Ge, Lu Yuan, Linwei Xu, Fan Yang, Wenhua Xu, Changmin Niu, Guanghua Li, Huiping Zhou, Ying Zheng","doi":"10.1093/biolre/ioae012","DOIUrl":"10.1093/biolre/ioae012","url":null,"abstract":"The centrosome is critical for maintaining the sperm head-tail connection and the formation of flagellar microtubules. In this study, we found that in mouse testes, CCDC159 (coiled-coil domain-containing protein 159) is specifically localized to the head-tail coupling apparatus (HTCA) of spermatids, a structure that ensures sperm head-tail tight conjunction. CCDC159 contains a C-terminal coiled-coil domain that functions as the centrosomal localization signal. Gene knockout (KO) of Ccdc159 in mice resulted in acephalic spermatozoa, abnormal flagella, and male infertility. To explore the mechanism behind CCDC159 regulating spermatogenesis, we identified CCDC159-binding proteins using a yeast two-hybrid screen and speculated that CCDC159 participates in HTCA assembly by regulating protein phosphatase PP1 activity. Further RNA-sequencing analyses of Ccdc159 KO testes revealed numerous genes involved in male gamete generation that were downregulated. Together, our results show that CCDC159 in spermatids is a novel centrosomal protein anchoring the sperm head to the tail. Considering the limitation of KO mouse model in clarifying the biological function of CCDC159 in spermatogenesis, a gene-rescue experiment will be performed in the future.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139485097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Foxp2 deficiency impairs reproduction by modulating the hypothalamic-pituitary-gonadal axis in zebrafish†. Foxp2 缺乏会通过调节斑马鱼的下丘脑-垂体-性腺轴而损害其生殖能力。

IF 3.6 2区生物学

Biology of Reproduction Pub Date : 2024-05-09 DOI: 10.1093/biolre/ioae019

Maya Wang, Juanjuan Luo, Lu Dai, Meilan Feng, Xiaoqian Cao, Jiannan Zhang, Yiping Wan, Xiaojun Yang, Yajun Wang

{"title":"Foxp2 deficiency impairs reproduction by modulating the hypothalamic-pituitary-gonadal axis in zebrafish†.","authors":"Maya Wang, Juanjuan Luo, Lu Dai, Meilan Feng, Xiaoqian Cao, Jiannan Zhang, Yiping Wan, Xiaojun Yang, Yajun Wang","doi":"10.1093/biolre/ioae019","DOIUrl":"10.1093/biolre/ioae019","url":null,"abstract":"FOXP2 was initially characterized as a transcription factor linked to speech and language disorders. Single-cell RNA sequencing reveals that Foxp2 is enriched in the gonadotrope cluster of the pituitary gland and colocalized with the hormones LHB and FSHB in chickens and mice, implying that FOXP2 might be associated with reproduction in vertebrates. Herein, we investigated the roles of foxp2 in reproduction in a Foxp2-deficient zebrafish model. The results indicated that the loss of Foxp2 inhibits courtship behavior in adult male zebrafish. Notably, Foxp2 deficiency disrupts gonad development, leading to retardation of follicle development and a decrease in oocytes in females at the full-growth stage, among other phenotypes. The transcriptome analysis (RNA-seq) also revealed that differentially expressed genes clustered into the estrogen signaling and ovarian steroidogenesis-related signaling pathways. In addition, we found that Foxp2 deficiency could modulate the hypothalamic-pituitary-gonadal axis, especially the regulation of lhb and fshb expression, in zebrafish. In contrast, the injection of human chorionic gonadotropin, a specific LH agonist, partially rescues Foxp2-impaired reproduction in zebrafish, suggesting that Foxp2 plays an important role in the regulation of reproduction via the hypothalamic-pituitary-gonadal axis in zebrafish. Thus, our findings reveal a new role for Foxp2 in the regulation of reproduction in vertebrates.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139575259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Activation of HAND2-FGFR signaling pathway by lncRNA HAND2-AS1 in adenomyosis. 更正：lncRNA HAND2-AS1 在子宫腺肌病中激活 HAND2-FGFR 信号通路。

IF 3.6 2区生物学

Biology of Reproduction Pub Date : 2024-05-09 DOI: 10.1093/biolre/ioae037

引用次数: 0

The pathogenesis of endometriosis and adenomyosis: insights from single-cell RNA sequencing†. 子宫内膜异位症和子宫腺肌症的发病机制：单细胞 RNA 测序的启示。

IF 3.6 2区生物学

Biology of Reproduction Pub Date : 2024-05-09 DOI: 10.1093/biolre/ioae032

Qiutong Li, Jinghua Shi, Dai Yi, Xiaoyan Li, Zhiyue Gu, Hailan Yan, Jinhua Leng

{"title":"The pathogenesis of endometriosis and adenomyosis: insights from single-cell RNA sequencing†.","authors":"Qiutong Li, Jinghua Shi, Dai Yi, Xiaoyan Li, Zhiyue Gu, Hailan Yan, Jinhua Leng","doi":"10.1093/biolre/ioae032","DOIUrl":"10.1093/biolre/ioae032","url":null,"abstract":"Endometriosis and adenomyosis are two similar gynecological diseases that are characterized by ectopic implantation and the growth of the endometrial tissue. Previous studies have reported that they share a common pathophysiology in some respects, such as a similar cellular composition and resistance to the progestogen of lesions, but their underlying mechanisms remain elusive. Emerging single-cell ribonucleic acid sequencing (scRNA-seq) technologies allow for the dissection of single-cell transcriptome mapping to reveal the etiology of diseases at the level of the individual cell. In this review, we summarized the published findings in research on scRNA-seq regarding the cellular components and molecular profiles of diverse lesions. They show that epithelial cell clusters may be the vital progenitors of endometriosis and adenomyosis. Subclusters of stromal cells, such as endometrial mesenchymal stem cells and fibroblasts, are also involved in the occurrence of endometriosis and adenomyosis, respectively. Moreover, CD8+ T cells, natural killer cells, and macrophages exhibit a deficiency in clearing the ectopic endometrial cells in the immune microenvironment of endometriosis. It seems that the immune responses are activated in adenomyosis. Understanding the immune characteristics of adenomyosis still needs further exploration. Finally, we discuss the application of findings from scRNA-seq for clinical diagnosis and treatment. This review provides fresh insights into the pathogenesis of endometriosis and adenomyosis as well as the therapeutic targets at the cellular level.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139929944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of early spermatogenesis in the giant prawn Macrobrachium rosenbergii by a GCL homolog†. GCL 同源物对大对虾早期精子发生的调控

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-05-09 DOI: 10.1093/biolre/ioae028

Jonathan Molcho, Dana Albagly, Tom Levy, Rivka Manor, Eliahu D Aflalo, Jorge Alfaro-Montoya, Amir Sagi

{"title":"Regulation of early spermatogenesis in the giant prawn Macrobrachium rosenbergii by a GCL homolog†.","authors":"Jonathan Molcho, Dana Albagly, Tom Levy, Rivka Manor, Eliahu D Aflalo, Jorge Alfaro-Montoya, Amir Sagi","doi":"10.1093/biolre/ioae028","DOIUrl":"10.1093/biolre/ioae028","url":null,"abstract":"The germ cell-less gene is crucial for gonad development in various organisms. Early interventions in its expression suggested a regulatory role at the mitotic stages of spermatogenesis, and its early knockout resulted in complete sterility in Drosophila. Genomic and transcriptomic data available for the catadromous giant prawn Macrobrachium rosenbergii enabled the identification of a germ cell-less homolog for this species, which we termed MroGCL (mRNA accession number OQ533056). An open reading frame containing 494 amino acids and a typical evolutionarily conserved BTB/POZ domain suggests possible protein-protein interaction functions in keeping with the Drosophila germ cell-less protein. Genomic mapping of MroGCL showed a full length of 120 896 bases. Analysis of the temporal expression of MroGCL showed constant expression in early prawn embryonic and larval stages, but a significant increase 10 days after metamorphosis when crucial sexual differentiation processes occur in prawns. In adult animals, high expression was detected in the gonads compared to the somatic tissues. RNAi-based knock-down experiments showed that both the silenced and control groups reached advanced spermatogenic stages, but that there was a significant decrease in the yield of spermatozoa in about half of the silenced animals. This finding supports our hypothesis that MroGCL is crucial for mitosis during early stage spermatogenesis. In conclusion, this study contributes to the understanding of crustacean gonad development and provides a stepping stone in the development of environmentally valuable sterile crustacean populations.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11094379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139970878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and comparison of human term placenta-derived trophoblast cells†. 人类胎盘滋养层细胞的建立和比较

IF 3.6 2区生物学

Biology of Reproduction Pub Date : 2024-05-09 DOI: 10.1093/biolre/ioae026

Manuel S Vidal, Enkhtuya Radnaa, Natasha Vora, Kamil Khanipov, Cristina Antich, Marc Ferrer, Rheanna Urrabaz-Garza, Jeena E Jacob, Ramkumar Menon

{"title":"Establishment and comparison of human term placenta-derived trophoblast cells†.","authors":"Manuel S Vidal, Enkhtuya Radnaa, Natasha Vora, Kamil Khanipov, Cristina Antich, Marc Ferrer, Rheanna Urrabaz-Garza, Jeena E Jacob, Ramkumar Menon","doi":"10.1093/biolre/ioae026","DOIUrl":"10.1093/biolre/ioae026","url":null,"abstract":"Research on the biology of fetal-maternal barriers has been limited by access to physiologically relevant cells, including trophoblast cells. In this study, we describe the development of a human term placenta-derived cytotrophoblast immortalized cell line (hPTCCTB) derived from the basal plate. Human-term placenta-derived cytotrophoblast immortalized cell line cells are comparable to their primary cells of origin in terms of morphology, marker expression, and functional responses. We demonstrate that these can transform into syncytiotrophoblast and extravillous trophoblasts. We also compared the hPTCCTB cells to immortalized chorionic trophoblasts (hFM-CTC), trophoblasts of the chorionic plate, and BeWo cells, choriocarcinoma cell lines of conventional use. Human-term placenta-derived cytotrophoblast immortalized cell line and hFM-CTCs displayed more similarity to each other than to BeWos, but these differ in syncytialization ability. Overall, this study (1) demonstrates that the immortalized hPTCCTB generated are cells of higher physiological relevance and (2) provides a look into the distinction between the spatially distinct placental and fetal barrier trophoblasts cells, hPTCCTB and hFM-CTC, respectively.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139705992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An interview with Professor Sally Camper. 采访莎莉-坎普教授

IF 3.6 2区生物学

Biology of Reproduction Pub Date : 2024-05-09 DOI: 10.1093/biolre/ioae054

T Rajendra Kumar

引用次数: 0

Prenatal exposure to Di(2-ethylhexyl) phthalate and high-fat diet synergistically disrupts gonadal function in male mice†. 产前接触 DEHP 和高脂肪饮食会协同破坏雄性小鼠的性腺功能。

IF 3.6 2区生物学

Biology of Reproduction Pub Date : 2024-05-09 DOI: 10.1093/biolre/ioae029

Radwa Barakat, Po-Ching Patrick Lin, Mary Bunnell, Ji-Eun Oh, Saniya Rattan, Cyrus Arnieri, Jodi A Flaws, CheMyong J Ko

{"title":"Prenatal exposure to Di(2-ethylhexyl) phthalate and high-fat diet synergistically disrupts gonadal function in male mice†.","authors":"Radwa Barakat, Po-Ching Patrick Lin, Mary Bunnell, Ji-Eun Oh, Saniya Rattan, Cyrus Arnieri, Jodi A Flaws, CheMyong J Ko","doi":"10.1093/biolre/ioae029","DOIUrl":"10.1093/biolre/ioae029","url":null,"abstract":"Prenatal exposure to Di (2-ethylhexyl) phthalate (DEHP) impairs the reproductive system and causes fertility defects in male offspring. Additionally, high-fat (HF) diet is a risk factor for reproductive disorders in males. In this study, we tested the hypothesis that prenatal exposure to a physiologically relevant dose of DEHP in conjunction with HF diet synergistically impacts reproductive function and fertility in male offspring. Female mice were fed a control or HF diet 7 days prior to mating and until their litters were weaned on postnatal day 21. Pregnant dams were exposed to DEHP or vehicle from gestational day 10.5 until birth. The male offspring's gross phenotype, sperm quality, serum hormonal levels, testicular histopathology, and testicular gene expression pattern were analyzed. Male mice born to dams exposed to DEHP + HF had smaller testes, epididymides, and shorter anogenital distance compared with those exposed to HF or DEHP alone. DEHP + HF mice had lower sperm concentration and motility compared with DEHP mice. Moreover, DEHP + HF mice had more apoptotic germ cells, fewer Leydig cells, and lower serum testosterone levels than DEHP mice. Furthermore, testicular mRNA expression of Dnmt1 and Dnmt3a was two to eight-fold higher than in DEHP mice by qPCR, suggesting that maternal HF diet and prenatal DEHP exposure additively impact gonadal function by altering the degree of DNA methylation in the testis. These results suggest that the combined exposure to DEHP and high-fat synergistically impairs reproductive function in male offspring, greater than exposure to DEHP or HF diet alone.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11094389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139929943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclophosphamide activates ferroptosis-induced dysfunction of Leydig cells via SMAD2 pathway†. 环磷酰胺通过 SMAD2 通路激活铁蛋白沉积诱导的 Leydig 细胞功能障碍。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-05-09 DOI: 10.1093/biolre/ioae020

Senlin Liao, Cun Wei, Guanyang Wei, Haoyu Liang, Fan Peng, Lei Zhao, Ziguang Li, Cundong Liu, Qizhao Zhou

{"title":"Cyclophosphamide activates ferroptosis-induced dysfunction of Leydig cells via SMAD2 pathway†.","authors":"Senlin Liao, Cun Wei, Guanyang Wei, Haoyu Liang, Fan Peng, Lei Zhao, Ziguang Li, Cundong Liu, Qizhao Zhou","doi":"10.1093/biolre/ioae020","DOIUrl":"10.1093/biolre/ioae020","url":null,"abstract":"Cyclophosphamide (CP) is a widely used chemotherapeutic drug and immunosuppressant in the clinic, and the hypoandrogenism caused by CP is receiving more attention. Some studies found that ferroptosis is a new mechanism of cell death closely related to chemotherapeutic drugs and plays a key role in regulating reproductive injuries. The purpose of this study is to explore ferroptosis' role in testicular Leydig cell dysfunction and molecular mechanisms relating to it. In this study, the level of ferroptosis in the mouse model of testicular Leydig cell dysfunction induced by CP was significantly increased and further affected testosterone synthesis. The ferroptosis inhibitors ferrostatin-1 (Fer-1) and iron chelator deferoxamine (DFO) can improve injury induced by CP. The results of immunohistochemistry showed that Fer-1 and DFO could improve the structural disorder of seminiferous tubules and the decrease of the number of Leydig cells in testicular tissue induced by CP. Immunofluorescence and western blot confirmed that Fer-1 and DFO could improve the expression of key enzymes in testosterone synthesis. The activation of SMAD family member 2 (Smad2)/cyclin-dependent kinase inhibitor 1A (Cdkn1a) pathway can improve the ferroptosis of Leydig cells induced by CP and protect the function of Leydig cells. By inhibiting the Smad2/Cdkn1a signal pathway, CP can regulate ferroptosis, resulting in testicular Leydig cell dysfunction. In this study, CP-induced hypoandrogenism is explained theoretically and a potential therapeutic strategy is provided.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139696841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of miRNAs in glucose metabolism of mouse cumulus cells†. miRNA 在小鼠精原细胞葡萄糖代谢中的作用

IF 3.6 2区生物学

Biology of Reproduction Pub Date : 2024-05-09 DOI: 10.1093/biolre/ioae013

Rui-Jie Ma, Min Zhang, Jia-Shun Wu, Zhi-Peng Wang, Guo-Liang Wang, Nan He, Ming-Jiu Luo, Jing-He Tan

{"title":"Role of miRNAs in glucose metabolism of mouse cumulus cells†.","authors":"Rui-Jie Ma, Min Zhang, Jia-Shun Wu, Zhi-Peng Wang, Guo-Liang Wang, Nan He, Ming-Jiu Luo, Jing-He Tan","doi":"10.1093/biolre/ioae013","DOIUrl":"10.1093/biolre/ioae013","url":null,"abstract":"It is known that the oocyte has a limited capacity to acquire and metabolize glucose, and it must rely on cumulus cells (CCs) to take up glucose and produce pyruvate for use to produce ATP through oxidative phosphorylation. We therefore propose that miRNAs might regulate glucose metabolism (GM) in CCs and might be used as markers for oocyte quality assessment. Here, mouse CC models with impaired glycolysis or pentose phosphate pathway (PPP) were established, and miRNAs targeting the key enzymes in glycolysis/PPP were predicted using the miRNA target prediction databases. Expression of the predicted miRNAs was compared between CCs with normal and impaired glycolysis/PPP to identify candidate miRNAs. Function of the candidate miRNAs was validated by transfecting CCs or cumulus-oocyte-complexes (COCs) with miRNA inhibitors and observing effects on glucose metabolites of CCs and on competence of oocytes. The results validated that miR-23b-3p, let-7b-5p, 34b-5p and 145a-5p inhibited glycolysis, and miR-24-3p, 3078-3p,183-5p and 7001-5p inhibited PPP of CCs. Our observation using a more physiologically relevant model (intact cultured COCs) further validated the four glycolysis-targeting miRNAs we identified. Furthermore, miR-let-7b-5p, 34b-5p and 145a-5p may also inhibit PPP, as they decreased the production of glucose-6-phosphate. In conclusion, miRNAs play critical roles in GM of CCs and may be used as markers for oocyte quality assessment. Summary sentence: We identified and validated eight new miRNAs that inhibit glycolysis and/or pentose phosphate pathways in cumulus cells (CCs) suggesting that miRNAs play critical roles in glucose metabolism of CCs and may be used for oocyte quality markers.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139545355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0