Biology of Reproduction最新文献_第9页

The research of lactylation in the field of reproduction†. 生殖领域的乳酸化研究。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-14 DOI: 10.1093/biolre/ioag133

Yunxuan Huang, Yaping Liang, Jing Chen

{"title":"The research of lactylation in the field of reproduction†.","authors":"Yunxuan Huang, Yaping Liang, Jing Chen","doi":"10.1093/biolre/ioag133","DOIUrl":"10.1093/biolre/ioag133","url":null,"abstract":"Lactate, once considered merely the product of glycolysis, is now recognized as both a metabolic fuel and a signaling molecule with broad physiological and pathological roles. The landmark discovery of lysine lactylation in 2019 unveiled a novel, direct mechanism through which lactate regulates cellular function. This dynamic posttranslational modification, installed by \"writers\" enzymes and removed by \"erasers,\" modulates the activity of histone and non-histone proteins, thereby influencing gene transcription, metabolism, and signal transduction. In reproductive biology, lactylation may represent an important regulator of fundamental processes including gametogenesis, embryo development, implantation, and placentation. Notably, aberrant lactylation is increasingly implicated in the pathogenesis of diverse reproductive disorders such as polycystic ovary syndrome, asthenozoospermia, ovarian cancer, preeclampsia, and recurrent pregnancy loss. This review systematically synthesizes current knowledge on the molecular mechanisms and functional significance of lactylation within the reproductive system, under both physiological and pathological contexts. Furthermore, we discuss the translational potential of targeting the lactylation pathway for novel therapeutic strategies and highlight key directions for future research.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"670-686"},"PeriodicalIF":3.2,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148337719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of endocrine disrupting chemicals diethylstilbestrol and ketoconazole on lipid storage in in vitro matured bovine cumulus-oocyte complexes†. 内分泌干扰物己烯雌酚和酮康唑对体外成熟牛卵丘细胞复合物脂质储存的影响。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-14 DOI: 10.1093/biolre/ioag119

Konstantina Asimaki, Chris H A Lest van de, Richard Wubbolts, Jeroen W A Jansen, J Bernd Helms, Hilde Aardema, Majorie B M Duursen van, Barend M Gadella

{"title":"The impact of endocrine disrupting chemicals diethylstilbestrol and ketoconazole on lipid storage in in vitro matured bovine cumulus-oocyte complexes†.","authors":"Konstantina Asimaki, Chris H A Lest van de, Richard Wubbolts, Jeroen W A Jansen, J Bernd Helms, Hilde Aardema, Majorie B M Duursen van, Barend M Gadella","doi":"10.1093/biolre/ioag119","DOIUrl":"10.1093/biolre/ioag119","url":null,"abstract":"Endocrine disrupting chemicals (EDCs) impact female fertility, and their widespread presence demands deeper understanding of their effects on reproduction. In vitro maturation of bovine cumulus-oocyte complexes (COCs) serves as a relevant reproductive toxicology model aligned with the 3R principle. Given the link between lipid metabolism and COC quality, the possibility that lipid droplets (LDs) in cumulus cells (CCs) and overall COC lipid content are susceptible to EDC disruption was tested. COCs were exposed to diethylstilbestrol (DES; estrogen receptor agonist) and ketoconazole (KTZ; steroidogenesis inhibitor), during in vitro maturation. Neutral lipid dyes were used for LD visualization (count, size, area, and clustering) in CCs. Lipidomic analysis using liquid chromatography-mass spectrometry was conducted separately on oocytes and CCs. Of all tested DES concentrations (i.e., 10-9 M, 10-7 M, 10-5 M), 10-5 M DES reduced the total LD area per CC, associated with decreased LD count and increased size. KTZ at all tested concentrations (i.e., 10-8 M, 10-7 M, 10-6 M) reduced LD size and enhanced clustering. Lipidomic analysis revealed the selective depletion of cholesteryl esters in CCs exposed to 10-5 M DES or 10-6 M KTZ, with minimal changes in other neutral lipid classes. Thus EDCs can disrupt lipid storage and LD dynamics in bovine COCs. We discuss these new findings with our previous work: (i) DES-induced steroidogenesis while (ii) in contrast KTZ blocked steroidogenesis in CCs. Together, the data highlight the COC lipid metabolism as a novel EDC target, warranting further study due to potential implications for female fertility.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"968-985"},"PeriodicalIF":3.2,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13578495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148161567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

O-GlcNAcylation of p66Shc drives senescence-associated secretory phenotype via mitochondrial DNA release in gestational diabetes mellitus†. 妊娠糖尿病患者o - glcn酰化p66Shc通过线粒体DNA释放驱动衰老相关分泌表型

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-14 DOI: 10.1093/biolre/ioag090

Hengxuan Zhu, Lulu Ji, Rujie Lai, Yaru Nai, Xiting Yang, Yuexiao Wang, Yanyi Huang, Qiongtao Wang, Hanyang Hu, Lin Wang

{"title":"O-GlcNAcylation of p66Shc drives senescence-associated secretory phenotype via mitochondrial DNA release in gestational diabetes mellitus†.","authors":"Hengxuan Zhu, Lulu Ji, Rujie Lai, Yaru Nai, Xiting Yang, Yuexiao Wang, Yanyi Huang, Qiongtao Wang, Hanyang Hu, Lin Wang","doi":"10.1093/biolre/ioag090","DOIUrl":"10.1093/biolre/ioag090","url":null,"abstract":"Chronic low-grade inflammation in gestational diabetes mellitus is one of the significant causes of placental dysfunction. However, the mechanisms by which abnormal glucose metabolism affects inflammation are not fully understood. p66Shc serves as a redox enzyme to regulate reactive oxygen species in mitochondria. Herein, we explore the interaction between p66Shc and OGT. High glucose induces O-GlcNAcylation of p66Shc, which promotes its protein stability and mitochondrial translocation, which induced elevated cytoplasmic mtDNA release and activation of the cGAS/STING to facilitate cellular senescence and inflammation in trophoblast cells. Specifically, we identify Ser213 may serve as the major site of O-GlcNAcylation on p66Shc. Mutation of Ser213 inhibits the activity of p66Shc, leading to compromised mtDNA release and senescence-associated secretory phenotype levels. We also identified hundreds of genes associated with inflammatory responses and cell cycle transition that are co-regulated by p66Shc and OGT, the deletion of either of which results in diminished level of senescence-associated secretory phenotype. Collectively, our findings highlight the vital role of O-GlcNAcylation in regulating p66Shc and senescence-associated secretory phenotype levels under high glucose, thereby underscoring its potential as a target for therapeutic intervention against gestational diabetes mellitus.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"875-889"},"PeriodicalIF":3.2,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147873086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Placental and fetal responses to adrenomedullin deficiency in the rat†. 大鼠肾上腺髓质素缺乏对胎盘和胎儿的影响。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-14 DOI: 10.1093/biolre/ioag125

Marija Kuna, Khursheed Iqbal, Kelly Gorman, Laszlo Balint, Keisuke Kozai, Masanaga Muto, Ayelen Moreno-Irusta, Marc Parrish, Kathleen M Caron, Michael J Soares

{"title":"Placental and fetal responses to adrenomedullin deficiency in the rat†.","authors":"Marija Kuna, Khursheed Iqbal, Kelly Gorman, Laszlo Balint, Keisuke Kozai, Masanaga Muto, Ayelen Moreno-Irusta, Marc Parrish, Kathleen M Caron, Michael J Soares","doi":"10.1093/biolre/ioag125","DOIUrl":"10.1093/biolre/ioag125","url":null,"abstract":"Adrenomedullin (ADM) is a peptide hormone with a broad spectrum of actions on vascular smooth muscle, endothelial cells, and immune cells. These ADM cellular targets are prominent constituents of the uterine-placental interface that undergo structural and functional transformation during pregnancy. To explore the biology of ADM at the uterine-placental interface, we examined ADM expression using in situ hybridization and generated and characterized an ADM mutant rat model using CRISPR-Cas9-mediated genome editing. At midgestation, Adm was prominently expressed in trophoblast giant cells. Adm expression shifted to the mesometrial uterus as gestation progressed. A germline Adm mutant model was generated. Adm heterozygous males and females were fertile; however, Adm heterozygous intercrosses did not generate live Adm null rats. Adm null fetal-placental sites were viable until gestation day (gd) 15.5. Fetal and placental growth restriction were evident on gd 13.5. On gd 15.5 some Adm null placentas exhibited prominent hemorrhagic regions, whereas both hemorrhage and edema were evident in gd 15.5 Adm null fetuses. Our results indicate that ADM serves as a regulator of placental and fetal development.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"911-922"},"PeriodicalIF":3.2,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13578494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148262977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Age-dependent testicular autophagy disruption mediates juvenile susceptibility to dibutyl phthalate-induced reproductive toxicity†. 年龄依赖性睾丸自噬破坏介导幼鱼对邻苯二甲酸二丁酯诱导的生殖毒性的易感性。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-14 DOI: 10.1093/biolre/ioag120

Huaming Xi, Feng Jiang, Xianglong Wang, Ziqian Wang, Yuan Li, Dong Niu

{"title":"Age-dependent testicular autophagy disruption mediates juvenile susceptibility to dibutyl phthalate-induced reproductive toxicity†.","authors":"Huaming Xi, Feng Jiang, Xianglong Wang, Ziqian Wang, Yuan Li, Dong Niu","doi":"10.1093/biolre/ioag120","DOIUrl":"10.1093/biolre/ioag120","url":null,"abstract":"Dibutyl phthalate (DBP) is a known reproductive toxicant, but the mechanisms underlying age-dependent susceptibility in the testis remain poorly defined. Here, we investigated how DBP exposure differentially affects testicular homeostasis in juvenile (3-week-old) versus adult (12-week-old) male mice, with a focus on autophagy regulation and its functional consequences for spermatogenesis. Juvenile and adult mice were orally administered DBP at 100 or 500 mg/kg/day for 35 days. We found that juvenile mice exhibited significantly greater testicular injury than adults, characterized by severe disruption of the seminiferous epithelium, Sertoli cell vacuolization, and compromised blood-testis barrier integrity. Mechanistically, DBP suppressed testicular autophagy-evidenced by reduced LC3-II/I ratio and nuclear translocation of TFEB-particularly in juvenile animals, leading to impaired mitochondrial quality control and accumulation of damaged organelles. Autophagy impairment was accompanied by robust NLRP3 inflammasome activation, elevated NF-κB phosphorylation, and diminished antioxidant capacity. Functionally, these molecular alterations were associated with decreased serum testosterone, luteinizing hormone and follicle-stimulating hormone levels, germ cell loss, and disrupted spermatogenic progression. Our findings demonstrate that autophagy serves as a critical protective mechanism in the developing testis, and its disruption underlies the heightened vulnerability of juvenile males to DBP-induced reproductive toxicity. These results advance our understanding of how environmental stressors perturb testicular physiology during critical windows of postnatal development.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"986-1004"},"PeriodicalIF":3.2,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obesity alters the estrogen-induced luteinizing hormone surge in ovariectomized ewes†. 肥胖改变卵巢切除母羊雌激素诱导的黄体生成素激增。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-14 DOI: 10.1093/biolre/ioag135

Trent Bronnenberg, Kelly S Kirkley, Christianne Magee, Dilyara A Murtazina, Whit C Stewart, Terry M Nett, Amy M Navratil, Jeremy D Cantlon, Brian D Cherrington, Colin M Clay

{"title":"Obesity alters the estrogen-induced luteinizing hormone surge in ovariectomized ewes†.","authors":"Trent Bronnenberg, Kelly S Kirkley, Christianne Magee, Dilyara A Murtazina, Whit C Stewart, Terry M Nett, Amy M Navratil, Jeremy D Cantlon, Brian D Cherrington, Colin M Clay","doi":"10.1093/biolre/ioag135","DOIUrl":"10.1093/biolre/ioag135","url":null,"abstract":"Obesity is a leading health issue with a negative effect on fertility, but the complexity of the relationship between obesity and fertility requires further study. Santoro and colleagues have characterized the metabolic environment of obesity and the development of reprometabolic syndrome. Due to the reproductive similarities between ewes and women, we use ovariectomized ewes as a translational model of reprometabolic syndrome and tested whether obesity alters the estradiol-17β (E2)-induced luteinizing hormone (LH) surge. Each ovariectomized ewe serves as her own biological control during the three phases of the study: normal weight (phase 1), obese (phase 2), and a return to normal weight (phase 3). Ewes were weighed weekly, and 12th rib fat thickness was measured as a proxy for body condition score. During each of the three phases of the study, following an intramuscular injection of 25 μg of E2, serial blood samples were collected, and serum LH concentrations were measured by radioimmunoassay. The peak concentration and timing of the LH surge are significantly lower and delayed in obese ewes in phase 2 as compared to phases 1 and 3. Our results suggest that the mechanisms that underlie the pathological effects of obesity and reprometabolic syndrome on the E2-induced LH surge are similar in ewes and women. Therefore, ewes can serve as a model for parsing the relative roles of diminished hypothalamic and pituitary sensitivity to E2, resulting in a reduced and delayed LH surge associated with obesity and reprometabolic syndrome in women.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"866-874"},"PeriodicalIF":3.2,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13578491/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148410283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spontaneous uterine and fetal recombination in TetO-Cre mice: implications for pregnancy studies†. TetO-Cre小鼠的自发性子宫和胎儿重组：对妊娠研究的启示。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-14 DOI: 10.1093/biolre/ioag142

Keisuke Kozai, Hruda N Malik, Gracy X Rosario, Sabita Dhal, Prashanth Anamthathmakula, Nihar R Nayak

引用次数: 0

A novel sperm swimming up trapper method enhances sperm quality and assisted reproductive technology outcomes by avoiding centrifugation-induced damage in a murine model†. 一种新的精子游动诱捕器方法通过避免离心诱导的小鼠模型损伤提高精子质量和ART结果。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-14 DOI: 10.1093/biolre/ioag127

Chun-Ru Song, Fang-Rui Xue, Chen Zhang, Kang Ding, Meng-Lei Feng, Dui Sun, Zhao-Yu Jiang, Qi Yang, Xiao-Yi Zhang, Wen-Ying Li, Yi-Tong Li, Cheng-Guang Liang

{"title":"A novel sperm swimming up trapper method enhances sperm quality and assisted reproductive technology outcomes by avoiding centrifugation-induced damage in a murine model†.","authors":"Chun-Ru Song, Fang-Rui Xue, Chen Zhang, Kang Ding, Meng-Lei Feng, Dui Sun, Zhao-Yu Jiang, Qi Yang, Xiao-Yi Zhang, Wen-Ying Li, Yi-Tong Li, Cheng-Guang Liang","doi":"10.1093/biolre/ioag127","DOIUrl":"10.1093/biolre/ioag127","url":null,"abstract":"Infertility affects approximately 15% of reproductive-age couples, with assisted reproductive technology (ART) serving as the primary therapeutic modality. The efficacy of sperm selection is critical for generating high-quality embryos; enriching for high-quality spermatozoa while minimizing iatrogenic damage markedly improves pregnancy outcomes. Conventional sperm preparation techniques, such as density gradient centrifugation (DGC), invariably involve centrifugation, which heightens the risk of sperm damage and may impair subsequent embryonic development. Here, we compared the efficacy and safety of a novel sperm swimming up trapper (SST) method with DGC for sperm isolation in a murine ART model. The procedure of SST approach is convenient, rapid, and obviates centrifugation, thereby mitigating sperm injury. Sperm recovered via SST displayed superior motility, morphology, DNA integrity, and fertilizing capacity relative to DGC-processed counterparts. Moreover, SST-isolated sperm exerted minimal deleterious effects on early embryo development and blastocyst quality, with no discernible impact on post embryo transfer offspring development. Collectively, these data establish SST as a safer, more effective, and streamlined sperm separation technique, providing foundational reference data for clinical sperm selection.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"954-967"},"PeriodicalIF":3.2,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vivo evidence for Urotensin II as a regulator of reproduction in GIFT tilapia (Oreochromis niloticus). 体内证据表明，尿紧张素II是GIFT罗非鱼（Oreochromis niloticus）繁殖的调节因子。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-12 DOI: 10.1093/biolre/ioag197

Ying Bai, Xiaozheng Yu, Jiahui Zhang, Yang Yu, Jinfeng Huang, Wensheng Li, Caiyun Sun

{"title":"In vivo evidence for Urotensin II as a regulator of reproduction in GIFT tilapia (Oreochromis niloticus).","authors":"Ying Bai, Xiaozheng Yu, Jiahui Zhang, Yang Yu, Jinfeng Huang, Wensheng Li, Caiyun Sun","doi":"10.1093/biolre/ioag197","DOIUrl":"https://doi.org/10.1093/biolre/ioag197","url":null,"abstract":"Urotensin II (UII) is a conserved vasoactive neuropeptide secreted by the caudal neurosecretory system (CNSS) of bony fish, yet its reproductive functions in teleost remain poorly defined. Here, we investigated the regulatory role of UII in the brain-pituitary-gonadal (BPG) axis of genetically improved farmed tilapia (GIFT, Oreochromis niloticus) using acute intraperitoneal injection, receptor antagonism with urantide, and sustained release via PLGA microspheres. Acute tUII administration significantly up-regulated mRNA expressions of gnrh1/2/3 in the brain, fshβ, lhβ, and gthα in the pituitary, and gonadal receptors (fshr, lhr), with dose-dependent biphasic responses. These stimulatory effects were abolished by urantide, confirming UT receptor-mediated signaling. Long-term delivery of tUII resulted in persistent activation of hypothalamic and pituitary genes, induction of steroidogenic and receptor genes in gonads, and significant elevation of serum estradiol in females and testosterone in males. Histological analyses further revealed accelerated spermatogenesis and oocyte maturation in tUII-treated groups. Moreover, UII up-regulated sex-differentiation genes, including dmrt1 in testis and foxl2 in ovaries, indicating its dual involvement in gonadal development and sexual differentiation. Together, these findings identify UII as a novel upstream regulator of tilapia reproductive axis and a potential target for manipulating puberty, gonadal maturation, and sex differentiation in aquaculture.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148954739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of high frequency administration of GnRH or kisspeptin on LH pulse and surge profiles in ovariectomized Bos taurus and Bos indicus heifers†. 高频率给药GnRH或kisspeptin对去卵巢牛牛和印度母牛LH脉冲和峰值的影响

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-11 DOI: 10.1093/biolre/ioag198

Lucas O eSilva, Sarah West, Viviana Garza, Jessica Sustaita-Monroe, Leslie King, Haley Sellers, Morgan Weynand, Hayden Feist, George Perry, Roberto Sartori, Rodolfo C Cardoso

{"title":"Effects of high frequency administration of GnRH or kisspeptin on LH pulse and surge profiles in ovariectomized Bos taurus and Bos indicus heifers†.","authors":"Lucas O eSilva, Sarah West, Viviana Garza, Jessica Sustaita-Monroe, Leslie King, Haley Sellers, Morgan Weynand, Hayden Feist, George Perry, Roberto Sartori, Rodolfo C Cardoso","doi":"10.1093/biolre/ioag198","DOIUrl":"https://doi.org/10.1093/biolre/ioag198","url":null,"abstract":"We hypothesized that high-frequency stimulation with kisspeptin or GnRH, mimicking increased GnRH pulsatility, would amplify the GnRH-induced LH surge in heifers. Additionally, LH pulse and surge parameters were compared between Bos taurus and Bos indicus heifers. Ovariectomized estradiol-replaced Hereford (n=6) and Brahman (n=8) heifers received two intravaginal progesterone devices on d -5. On d -0.5, jugular catheters were placed and on d 0 blood samples were collected every 15 min for 12 h. In a crossover design, each heifer received all three treatments in separate replicates, administered hourly during the first 8 h: Control = saline; Kisspeptin = 0.4 μg/kg of kisspeptin; or GnRH = 0.005 μg/kg of gonadorelin acetate. At 8 h, 100 μg of gonadorelin acetate was administered to induce an LH surge. Both kisspeptin and GnRH treatments effectively induced high-frequency LH pulses. Regardless of the genetic group, 75% of kisspeptin and 87.5% of GnRH injections resulted in detectable LH pulses. LH pulsatility was greater (P<0.01) in kisspeptin- and GnRH-treated heifers compared to controls (6.1±0.6, 7.0±0.4, 2.3±0.5 pulses/8 h; respectively). Nevertheless, neither endogenous nor treatment-induced LH pulse parameters differed between breeds. The GnRH-induced LH surge was not enhanced by the high-frequency stimulation, however, it was markedly reduced in Brahman compared to Hereford heifers (P<0.01). In conclusion, the high-frequency GnRH stimulation did not enhance LH surge response to a GnRH challenge. Although LH pulsatile secretion was similar, differential pituitary responsiveness to a GnRH ovulatory stimulus may contribute to differences in reproductive function between Bos taurus and Bos indicus heifers.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148944237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0