Biology of Reproduction最新文献_第8页

Near infrared LED Irradiation Reverses the Declining Quality of Mice Post-Ovulatory Aging and Reproductive Aged Oocytes. 近红外LED照射逆转小鼠排卵后老化和生殖老化卵母细胞质量下降。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-06-12 DOI: 10.1093/biolre/ioaf129

Na Zhang, Yan You, Yanhua Zhao, Ying Lian, Xiulian Ren, Zhongwei Wang, Yapeng Wang, JianGen Xu, Lei Lei, Jianfei Dong, Ping Liu

{"title":"Near infrared LED Irradiation Reverses the Declining Quality of Mice Post-Ovulatory Aging and Reproductive Aged Oocytes.","authors":"Na Zhang, Yan You, Yanhua Zhao, Ying Lian, Xiulian Ren, Zhongwei Wang, Yapeng Wang, JianGen Xu, Lei Lei, Jianfei Dong, Ping Liu","doi":"10.1093/biolre/ioaf129","DOIUrl":"https://doi.org/10.1093/biolre/ioaf129","url":null,"abstract":"The advanced maternal age is strongly correlated with a notable decline in oocyte quality, yet definitive and effective strategies to enhance it remain incompletely identified. In this study, we reported that near-infrared light, administered in vitro, efficaciously improves the quality of post-ovulatory aging (POA) and reproductive aged oocytes by recovering mitochondrial activity. Near-infrared light has the capacity to ameliorate abnormalities in mitochondrial membrane potential, optimize mitochondrial distribution, augment ATP synthesis, and modulate the expression of mitochondrial-related genes. It can consequently enhance the quality of oocytes by reducing the reactive oxygen species (ROS) level, improving the abnormal distribution of spindles and maintaining the fertilization ability. Moreover, transcriptome analysis also shows that the beneficial effect of near-infrared light on POA and reproductive aged oocytes is mediated by restoration of mitochondrial function. Collectively, our data reveal that the near-infrared light treatment, especially of 810 nm or 950 nm with a cumulative dosage of 1.5 J*cm-2, is a feasible approach to protect oocytes from POA and reproductive aged deterioration, contributing to the improvement of reproductive outcomes of aged women and assisted reproductive technology.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Programmed DNA elimination occurs during early gametogenesis in hybridogenetic hybrids from Hexagrammos genus. DNA的程序化消除发生在杂交杂交种的配子体发生早期。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-06-06 DOI: 10.1093/biolre/ioaf125

Dmitrij Dedukh, Kenta Nitsu, Takafumi Fujimoto, Shota Suzuki, Hiroyuki Munehara

{"title":"Programmed DNA elimination occurs during early gametogenesis in hybridogenetic hybrids from Hexagrammos genus.","authors":"Dmitrij Dedukh, Kenta Nitsu, Takafumi Fujimoto, Shota Suzuki, Hiroyuki Munehara","doi":"10.1093/biolre/ioaf125","DOIUrl":"https://doi.org/10.1093/biolre/ioaf125","url":null,"abstract":"Hybridogenesis is a unique type of reproduction found in hybrids, producing offspring that are partial (hemiclonal) genetic replicas of one parent. Along the southern coast of Hokkaido, Japan, two types of interspecies hybrids have been identified among three Hexagrammos species: Hexagrammos octogrammus (Hoc), H. otakii (Hot), and Hexagrammos agrammus (Hag). These hybrids are characterized by fertile females reproducing via hybridogenesis. During oogenesis of hybrids, the paternal haploid genome (Hag or Hot) is excluded, while only the maternal genome (Hoc) retains and transmits to gametes. This study investigates the mechanism of genome elimination in Hexagrammos hybrids through a comparative analysis of gonadal development in one-year-old juveniles and adult individuals of hybrids and their parental species. Comparative genomic hybridization on whole-mount gonads from Hoc/Hot and (Hoc/Hag) × Hag hybrids revealed gonocytes containing genomes of both parental species and gonocytes with only Hoc genome, suggesting that genome elimination occurs during early gametogenesis. In Hoc but not in Hot or Hag species, we observed heterochromatin foci enriched with H3K9me3 epigenetic histone modification. Similar foci were found in hybrids, confirming the presence of Hoc genome in their gonocytes. Furthermore, we detected micronuclei in the cytoplasm of gonocytes in parental species and hybrids. Although micronuclei were rare, their frequency was significantly higher in hybrids compared to parental species. Within micronuclei in Hoc/Hot and (Hoc/Hag) × Hag hybrids, we identified Hot and Hag chromosomes or their fragments, respectively. Thus, in Hexagrammos hybrids, genome elimination likely occurs during early gametogenic stages and is accompanied by micronuclei formation.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding the Maternal-Fetal Dialogue: NK Cell Education in Reproductive Outcomes†. 解码母胎对话：NK细胞教育在生殖结果†。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-06-06 DOI: 10.1093/biolre/ioaf124

Zhuoxu He, Dong Liu, Yueli Mu, Hong Li

{"title":"Decoding the Maternal-Fetal Dialogue: NK Cell Education in Reproductive Outcomes†.","authors":"Zhuoxu He, Dong Liu, Yueli Mu, Hong Li","doi":"10.1093/biolre/ioaf124","DOIUrl":"https://doi.org/10.1093/biolre/ioaf124","url":null,"abstract":"Natural killer (NK) cell education, a dynamic and finely tuned process, is essential for calibrating decidual NK (dNK) cell responsiveness at the maternal-fetal interface. This education process, governed by distinct receptor-ligand interactions, refines dNK cell function to maintain immune tolerance while preserving responsiveness to pathological threats. Killer-cell immunoglobulin-like receptors (KIRs) interacting with human leukocyte antigen (HLA)-C dictate a balance between activation and inhibition, shaping dNK cell adaptability to maternal-fetal genetic variation. The NKG2A-HLA-E axis establishes a foundational inhibitory checkpoint, ensuring immune tolerance through metabolic and functional programming. LILRB1-HLA-G interactions promote immunomodulatory and angiogenic functions crucial for placental development, while SLAM family receptors (SFRs), including 2B4-CD48, modulate activation thresholds and restrain excessive cytotoxicity. Disruptions in these finely orchestrated pathways contribute to recurrent implantation failure, pregnancy loss, and preeclampsia. This review synthesizes the diverse mechanisms of NK cell education, highlighting how specific receptor-ligand interactions regulate dNK cell function and maternal-fetal immune adaptation. A deeper understanding of these pathways not only enhances our comprehension of reproductive immunology but also paves the way for novel therapeutic strategies targeting immune dysregulation in pregnancy-related disorders.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined treatment with IGF1, CoQ10 and melatonin improves the quality of bovine oocytes and heat-shocked blastocysts†. IGF1、CoQ10和褪黑素联合治疗可改善牛卵母细胞和热休克囊胚†的质量。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-06-05 DOI: 10.1093/biolre/ioaf123

Hang Zhang, Baigao Yang, Peipei Zhang, Jianhua Cao, Xiaomeng Zhang, Smhaa Gahallah, Zvi Roth, Pengcheng Wan, Xueming Zhao

{"title":"Combined treatment with IGF1, CoQ10 and melatonin improves the quality of bovine oocytes and heat-shocked blastocysts†.","authors":"Hang Zhang, Baigao Yang, Peipei Zhang, Jianhua Cao, Xiaomeng Zhang, Smhaa Gahallah, Zvi Roth, Pengcheng Wan, Xueming Zhao","doi":"10.1093/biolre/ioaf123","DOIUrl":"https://doi.org/10.1093/biolre/ioaf123","url":null,"abstract":"Heat stress significantly impairs cattle fertility, particularly affecting oocytes and developing blastocysts. Developing strategies to improve embryonic development under heat stress is crucial for advancing the cattle breeding industry. This study evaluated the effects of a combined treatment with insulin-like growth factor 1 (IGF1), coenzyme Q10 (CoQ10), and melatonin (MT) (i.e., IGF1+CoQ10+MT) on oocyte competence and genes expression in the formed blastocysts. Bovine in vitro fertilization (IVF) blastocysts were assessed under heat shock (41°C for 12 hours) and control conditions (38.5°C). The combined supplementation of IGF1 (100 ng/mL), CoQ10 (50 μM), and MT (1 μM) during in vitro maturation (IVM) and in vitro culture (IVC) improved oocyte quality and competence by reducing reactive oxygen species (ROS) levels and apoptosis, increasing mitochondrial membrane potential (ΔΨm), optimizing mitochondrial distribution, and upregulating quality related genes. Heat shock decreased ATP content, increased apoptosis, and affected gene expression related to development, apoptosis, ubiquitination, and autophagy, impacting blastocyst development. The combined treatment mitigated heat shock damage by enhancing ATP content, reducing apoptosis, and improving gene expression, thus improving blastocyst quality.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell analysis of ovarian myeloid cells identifies age associated changes in macrophages and signaling dynamics. 卵巢骨髓细胞的单细胞分析发现巨噬细胞和信号动力学的年龄相关变化。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-06-03 DOI: 10.1093/biolre/ioaf122

Zijing Zhang, Lu Huang, Lynae Brayboy, Michael Birrer

引用次数: 0

The Single and Combined Effects of Decabromodiphenyl Ethane and Mixed Microplastics on Male Mice Reproductive Toxicity. 十溴二苯乙烷和混合微塑料对雄性小鼠生殖毒性的单独和联合影响。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-05-31 DOI: 10.1093/biolre/ioaf121

Zhipeng Qi, Wei Li, Linwei Pan, Weiyi Song, Yu Deng, Jing Li, Lichun Xu, Jikuan Yan, Cheng Zou, Haibo Yang, Xinxin Yang

{"title":"The Single and Combined Effects of Decabromodiphenyl Ethane and Mixed Microplastics on Male Mice Reproductive Toxicity.","authors":"Zhipeng Qi, Wei Li, Linwei Pan, Weiyi Song, Yu Deng, Jing Li, Lichun Xu, Jikuan Yan, Cheng Zou, Haibo Yang, Xinxin Yang","doi":"10.1093/biolre/ioaf121","DOIUrl":"https://doi.org/10.1093/biolre/ioaf121","url":null,"abstract":"The expansive surface area of microplastics (MPs) allow them to retain pollutants, however, the combined toxicity of mixed MPs and organic contaminants remains poorly studied. The semen quality of men worldwide was observed to exhibit downward trends. This study investigates the reproductive toxicity and underlying mechanisms of male mice exposed to MPs and novel brominated flame retardants decabromodiphenyl ethane (DBDPE), individually or combined. Male mice were treated by oral gavage with corn oil, 5 or 50 mg/kg DBDPE, 10 mg/kg MPs (a mixture of PE (Polyethylene) and PVC (Polyvinyl chloride)), and DBDPE+MPs (50 mg/kg DBDPE mixed with 10 mg/kg MPs) for 7 weeks. Our results suggested DBDPE alone decreased the sex hormones levels and sperm count, whereas MPs alone had no significant effect. DBDPE and/or MPs impaired testicular morphology, sperm malformation rate, oxidative stress levels, and apoptosis. Our results of transcriptomics from GC-2 cells indicated that DBDPE and MPs lead to changes in oxidative stress, apoptosis, inflammation, proliferation and differentiation. Furthermore, DBDPE and/or MPs exposure inhibited the Kelch-like ECH-associated protein 1- NF-E2-related factor 2 (Keap1-Nrf2) signaling pathway. Moreover, the combined treatment of MPs and DBDPE exerted an antagonistic effect on the reproductive system of mice. This study provides valuable insights into the mechanisms of joint action in reproductive toxicity in mammals due to the combined exposure to MPs and DBDPE.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Embryo Culture and Day of Embryo Transfer on the Cardiometabolic Health of Adult Mice†. 胚胎培养和胚胎移植时间对成年小鼠心脏代谢健康的影响

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-05-31 DOI: 10.1093/biolre/ioaf120

Rhodel K Simbulan, Seok Hee Lee, Reza K Oqani, Xiaowei Liu, Louise Lantier, Owen P McGuinness, George A Brooks, Paolo F Rinaudo

{"title":"Impact of Embryo Culture and Day of Embryo Transfer on the Cardiometabolic Health of Adult Mice†.","authors":"Rhodel K Simbulan, Seok Hee Lee, Reza K Oqani, Xiaowei Liu, Louise Lantier, Owen P McGuinness, George A Brooks, Paolo F Rinaudo","doi":"10.1093/biolre/ioaf120","DOIUrl":"https://doi.org/10.1093/biolre/ioaf120","url":null,"abstract":"Assisted reproductive technologies (ART) have rapidly evolved since being introduced in 1978. However, many of the procedures used clinically lack a basis of long-term studies to ensure their safety. Though in vitro fertilization (IVF) is largely safe, follow up studies have shown that IVF-conceived children may show signs of altered fat deposition, increased fasting glucose and increased blood pressure. These results are, however, limited by low number of patients and different ART variables (different length of embryo culture, or different types of culture media). Results of studies using animal models have confirmed many of these results and shown that more stressful culture conditions result in disrupted adult phenotypes. Presently, it is unclear if culture conditions such as duration and day of transfer might affect adult health. To investigate the hypothesis that length of embryo culture could affect adult phenotype, we generated mice by IVF and transferred them at the cleavage stage (IVF8C group) or at the blastocyst stage, (IVFBL group) and studied adult phenotype. Results were compared to those obtained with naturally conceived animals flushed out of the uterus and transferred to recipient (FB group). We found a sexual dimorphic effects with male mice showing a more severe phenotype. Male offspring resulting from cleavage stage transfer showed altered glucose handling, left cardiac dysfunction and shorter lifespan, while male offspring post blastocyst transfer showed reduced locomotor activity. Female mice showed a milder phenotype, particular for female offspring generated by transfer at the cleavage stage.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Development of a Preclinical Swine Model for Endometriosis. 子宫内膜异位症猪临床前模型的建立。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-05-30 DOI: 10.1093/biolre/ioaf118

Harriet Omenge, Breton F Barrier, Kaylynn Monarch, Emily Kiesewetter, Sarah Schlink, Mariana Sponchiado, Randall S Prather, Rodney D Geisert, Taeho Kim, Jung-Ho Shin, Tae Hoon Kim, Kiho Lee, Jae-Wook Jeong

{"title":"The Development of a Preclinical Swine Model for Endometriosis.","authors":"Harriet Omenge, Breton F Barrier, Kaylynn Monarch, Emily Kiesewetter, Sarah Schlink, Mariana Sponchiado, Randall S Prather, Rodney D Geisert, Taeho Kim, Jung-Ho Shin, Tae Hoon Kim, Kiho Lee, Jae-Wook Jeong","doi":"10.1093/biolre/ioaf118","DOIUrl":"https://doi.org/10.1093/biolre/ioaf118","url":null,"abstract":"Endometriosis is a benign gynecological condition in which endometrial-like tissue grows outside of the uterus, and the mechanisms of its pathogenesis are not fully understood. The anatomical and physiological parallels between pigs and humans have made pigs a good model for investigating other human diseases. Here, we report on the development of a swine model for endometriosis in which the peritoneal cavity was inoculated with autologous endometrial tissue fragments labeled with fluorescein isothiocyanate dye-doped silica nanoparticles. After 6 weeks of endometriosis induction, FITC positive endometriotic lesions were observed on the peritoneal surface of the abdominal wall as well as on the serosal surfaces of the uterus and small intestine. Histological analysis of the endometriotic lesions revealed endometrial-like epithelial and stromal cells that were morphologically comparable to human endometriotic lesions. The identification of epithelial and stromal cells in these lesions was confirmed by immunostaining of e-cadherin, an endometrial epithelial marker, and Chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII), an endometrial stromal marker. Our results illustrate successful induction of endometriosis with fluorescent-labeled endometriotic lesions to identify in situ endometriotic lesions. According to the size and physiological similarity of pigs to humans, this is an important first step toward the development of a non-invasive tool to diagnose endometriosis. Furthermore, the availability of a new large animal model for translational endometriosis research provides a novel preclinical animal model for laparoscopic monitoring and retrieval of experimental endometriotic lesions with ample serum for serial analyses.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pluripotent Stem Cell Lines on the Farm: Naïve-State Pluripotency, Germline Chimerism, and Nuclear Transfer Cloning. 多能干细胞系在农场：Naïve-State多能性，种系嵌合，和核转移克隆。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-05-19 DOI: 10.1093/biolre/ioaf099

Worawalan Samruan, Hervé Acloque, Marielle Afanassieff, Rangsun Parnpai, Nathalie Beaujean, Pierre Savatier

引用次数: 0

Consequences of varying methionine concentrations on development of the bovine embryo in vitro†. 不同蛋氨酸浓度对牛体外胚胎发育的影响。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-05-19 DOI: 10.1093/biolre/ioaf111

Masroor Sagheer, Daniel Carballo, Tatiane S Maia, Peter J Hansen

{"title":"Consequences of varying methionine concentrations on development of the bovine embryo in vitro†.","authors":"Masroor Sagheer, Daniel Carballo, Tatiane S Maia, Peter J Hansen","doi":"10.1093/biolre/ioaf111","DOIUrl":"https://doi.org/10.1093/biolre/ioaf111","url":null,"abstract":"Methionine is an essential amino acid that influences key cellular processes. This study examined how methionine concentrations representing deficiency (0 μM), estimated uterine concentration in cows (31 μM), and a supra-physiological concentration (i.e. rumen-protected methionine; 57 μM) affect bovine preimplantation embryo development. When compared to 31 and 57 μM methionine, methionine deficiency (0 μM) reduced the percentage of zygotes developing into blastocysts by day 7.5 and the proportion of blastocysts that hatched at day 7.5, 8.5, and 9.5. Blastocysts produced without methionine developed slowly, had fewer total, CDX2+(trophectoderm), and SOX2+(inner cell mass) cells, and exhibited higher apoptosis and lipid content. Increasing methionine from 31 to 57 μM did not improve blastocyst development or hatching but increased CDX2+cells number and reduced apoptosis and lipid content. Methionine concentration had no effect on survival to vitrification. In conclusion, the absence of methionine reduces the competence of zygotes to become a blastocyst and has detrimental effects on the properties of those blastocysts. A higher methionine concentration increases trophectoderm cell number, reduces apoptosis, and lowers lipid accumulation. These findings suggest that nutritional strategies to increase methionine availability during the periconceptional period may enhance blastocyst competence for continued development.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0