Biology of Reproduction最新文献_第8页

Insight into the post-translational modifications in pregnancy and related complications†. 深入了解妊娠和相关并发症的翻译后修饰。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-02-14 DOI: 10.1093/biolre/ioae149

Yangxue Yin, Lingyun Liao, Qin Xu, Shuangshuang Xie, Liming Yuan, Rong Zhou

{"title":"Insight into the post-translational modifications in pregnancy and related complications†.","authors":"Yangxue Yin, Lingyun Liao, Qin Xu, Shuangshuang Xie, Liming Yuan, Rong Zhou","doi":"10.1093/biolre/ioae149","DOIUrl":"10.1093/biolre/ioae149","url":null,"abstract":"Successful pregnancy is dependent on a number of essential events, including embryo implantation, decidualization, and placentation. Failure of the above process may lead to pregnancy-related complications, including preeclampsia, gestational diabetes mellitus, preterm birth, and fetal growth restriction, may affect 15% of pregnancies, and lead to increased mortality and morbidity of pregnant women and perinatal infants, as well as the occurrence of short-term and long-term diseases. These complications have distinct etiology and pathogenesis, and the present comprehension is still lacking. Post-translational modifications are important events in epigenetics, altering the properties of proteins through protein hydrolysis or the addition of modification groups to one or more amino acids, with different modification states regulating subcellular localization, protein degradation, protein-protein interaction, signal transduction, and gene transcription. In this review, we focus on the impact of various post-translational modifications on the progress of embryo and placenta development and pregnancy-related complications, which will provide important experimental bases for exploring new insights into the physiology of pregnancy and pathogenesis associated with pregnancy complications.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"204-224"},"PeriodicalIF":3.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosomal ncRNAs in reproductive cancers†. 生殖系统癌症中的外泌体 ncRNA。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-02-14 DOI: 10.1093/biolre/ioae170

Alicja Kowalczyk, Marcjanna Wrzecińska, Elżbieta Gałęska, Ewa Czerniawska-Piątkowska, Mercedes Camiña, Jose P Araujo, Zbigniew Dobrzański

{"title":"Exosomal ncRNAs in reproductive cancers†.","authors":"Alicja Kowalczyk, Marcjanna Wrzecińska, Elżbieta Gałęska, Ewa Czerniawska-Piątkowska, Mercedes Camiña, Jose P Araujo, Zbigniew Dobrzański","doi":"10.1093/biolre/ioae170","DOIUrl":"10.1093/biolre/ioae170","url":null,"abstract":"Extracellular vesicles, particularly exosomes, play a pivotal role in the cellular mechanisms underlying cancer. This review explores the various functions of exosomes in the progression, growth, and metastasis of cancers affecting the male and female reproductive systems. Exosomes are identified as key mediators in intercellular communication, capable of transferring bioactive molecules such as microRNAs, proteins, and other nucleic acids that influence cancer cell behavior and tumor microenvironment interactions. It has been shown that non-coding RNAs transported by exosomes play an important role in tumor growth processes. Significant molecules that may serve as biomarkers in the development and progression of male reproductive cancers include miR-125a-5p, miR-21, miR-375, the miR-371 ~ 373 cluster, and miR-145-5p. For female reproductive cancers, significant microRNAs include miR-26a-5p, miR-148b, miR-205, and miRNA-423-3p. This review highlights the potential of these noncoding RNAs as biomarkers and prognostics in tumor diagnostics. Understanding the diverse roles of exosomes may hold promise for developing new therapeutic strategies and improving treatment outcomes for cancer patients.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"225-244"},"PeriodicalIF":3.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11833474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beta-hydroxybutyrate alters bovine preimplantation embryo development through transcriptional and epigenetic mechanisms†. β -羟基丁酸通过转录和表观遗传机制改变牛着床前胚胎发育†。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-02-14 DOI: 10.1093/biolre/ioae175

Juliano Rodrigues Sangalli, Ricardo Perecin Nociti, Marcos Roberto Chiaratti, Alessandra Bridi, Ramon Cesar Botigelli, Dewison Ricardo Ambrizi, Helena Fabiana Reis de Almeida Saraiva, Felipe Perecin, Juliano Coelho da Silveira, Pablo Juan Ross, Flávio Vieira Meirelles

{"title":"Beta-hydroxybutyrate alters bovine preimplantation embryo development through transcriptional and epigenetic mechanisms†.","authors":"Juliano Rodrigues Sangalli, Ricardo Perecin Nociti, Marcos Roberto Chiaratti, Alessandra Bridi, Ramon Cesar Botigelli, Dewison Ricardo Ambrizi, Helena Fabiana Reis de Almeida Saraiva, Felipe Perecin, Juliano Coelho da Silveira, Pablo Juan Ross, Flávio Vieira Meirelles","doi":"10.1093/biolre/ioae175","DOIUrl":"10.1093/biolre/ioae175","url":null,"abstract":"Developing embryos are susceptible to fluctuations in the nutrients and metabolites concentrations within the reproductive tract, which can lead to alterations in their developmental trajectory. Ketotic dairy cows have diminished fertility, and elevated levels of the ketone body beta-hydroxybutyrate (BHB) have been associated with poor embryonic development. We used an in vitro model based on either in vitro fertilization (IVF) or parthenogenesis to investigate the effects of BHB on the preimplantation bovine embryo development, epigenome, and transcriptome. Embryo culture medium was supplemented with BHB at a similar concentration to that present in the blood of cows suffering with severe ketosis, followed by analysis of blastocysts formation rate, diameter, total number of cells, levels of H3K9 beta-hydroxybutyrylation (H3K9bhb), apoptosis, and transcriptional alterations. As a result, we observed that BHB reduced the blastocysts rates, the diameter and the total number of cells in both parthenotes and IVF embryos. Exposure to BHB for either 3 or 7 days greatly increased the H3K9bhb levels in parthenotes at the 8-cells and blastocyst stages, and affected the expression of HDAC1, TET1, DNMT1, KDM6B, NANOG, and MTHFD2 genes. Additionally, culture of IVF embryos with BHB for 7 days dramatically increased H3K9bhb and reduced NANOG in blastocysts. RNA-seq analysis of IVF blastocysts revealed that BHB modulated the expression of 118 genes, which were involved with biological processes such as embryonic development, implantation, reproduction, proliferation, and metabolism. These findings provided valuable insights into the mechanisms through which BHB disrupts preimplantation embryonic development and affects the fertility in dairy cows.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"253-272"},"PeriodicalIF":3.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin modulated GPX5 and PTGDS expression in Bactrian camel epididymis mainly via receptor MT1.

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-02-14 DOI: 10.1093/biolre/ioaf030

Shuqin Zhao, Shipeng Wu, Shuai Ji, Yaxuan Han, Zhen Yang, Yuan Gao

{"title":"Melatonin modulated GPX5 and PTGDS expression in Bactrian camel epididymis mainly via receptor MT1.","authors":"Shuqin Zhao, Shipeng Wu, Shuai Ji, Yaxuan Han, Zhen Yang, Yuan Gao","doi":"10.1093/biolre/ioaf030","DOIUrl":"https://doi.org/10.1093/biolre/ioaf030","url":null,"abstract":"Melatonin (Mel), an important mediator of photoperiodic annual rhythm regulation and seasonal reproduction in animals, directly modulates the expression of specific genes in the epididymis and protects sperm from oxidative damage. Bactrian camel is a dominant species in desert and semi-desert areas, exhibiting the unique reproductive regulation patterns. However, the underlying regulation mechanism of Mel on Bactrian camel is still unclear. This study isolated the epididymal caput epithelial cells of Bactrian camels and investigated the expression of specific genes involving sperm protection after Mel treatment and overexpression / knockdown of Mel receptor MT1/MT2 using qPCR, ELISA, and western blotting assay. The results showed that MT1, MT2, clock genes cryptochrome 1/2 (Cry1/Cry2) were all positively expressed in the epididymal lumen epithelial cells, peritubular myoid cells, and luminal spermatozoa. Intriguingly, Mel treatment activated receptor MT1 in epididymal caput epithelial cells, indicating that Mel treatment regulated genes expression mainly via MT1-dependent manner. Mel treatment or overexpression of MT1 both increased secretion of glutathione peroxidase 5 (GPX5) and prostaglandin D2 synthase (PTGDS), and MT1 silencing induced downregulation of GPX5 and PTGDS expression, indicating that the expression of GPX5 and PTGDS were regulated by Mel-MT1. Overexpression of MT1 or MT2 promoted Cry2 expression, and overexpression of Cry2 also activated the MT1/MT2 expression by feedback regulation. Finally, the double luciferase reports assay showed that the activation of MT1 by Cry2 occurred during transcription. These results help to understand the regulatory effect of Mel on the epididymis in Bactrian camels.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143416943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visfatin exerts an anti-proliferative and pro-apoptotic effect in the human placenta cells†. Visfatin 对人类胎盘细胞具有抗增殖和促凋亡作用。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-02-14 DOI: 10.1093/biolre/ioae168

Monika Dawid, Karolina Pich, Natalia Respekta-Długosz, Wiktoria Gieras, Małgorzata Opydo, Tomasz Milewicz, Pascal Froment, Joëlle Dupont, Agnieszka Rak

{"title":"Visfatin exerts an anti-proliferative and pro-apoptotic effect in the human placenta cells†.","authors":"Monika Dawid, Karolina Pich, Natalia Respekta-Długosz, Wiktoria Gieras, Małgorzata Opydo, Tomasz Milewicz, Pascal Froment, Joëlle Dupont, Agnieszka Rak","doi":"10.1093/biolre/ioae168","DOIUrl":"10.1093/biolre/ioae168","url":null,"abstract":"Visfatin regulates energy homeostasis, metabolism, inflammation, and reproduction via the hypothalamus-pituitary-ovary axis. Our previous study showed the visfatin gene and protein expression in the human placenta. This study aimed to investigate the in vitro effect of visfatin on the proliferation and apoptosis of placental JEG-3 and BeWo cells but also in villous explants collected from normal pregnancies and complicated by intrauterine growth restriction (IUGR), preeclampsia (PE), and gestational diabetes mellitus (GDM). We studied placenta cells viability, proliferation, cell cycle, proliferation/apoptotic factors and insulin receptor (INSR) expression, DNA fragmentation, CASP3/7 activity, and phosphorylation of ERK1/2, AKT, AMPKα, STAT3 with their involvement after pharmacological inhibition in visfatin action on proliferation and apoptosis. Visfatin (1, 10, 100 ng/mL) decreased the viability and proliferation of JEG-3 after 48 h, and a similar effect was observed via co-administration of visfatin (10 ng/mL) and insulin (10 ng/mL) in JEG-3 and BeWo after 48 h and 72 h, respectively. Visfatin reduced the transition from the G2/M phase, and expression of PCNA or cyclins D, E, A, and B in JEG-3 and PCNA in normal, IUGR, PE, and GDM placentas. It increased DNA fragmentation, CASP3/7 activity, P53, BAX/BCL2, CASP9, CASP 8, CASP3 levels in BeWo, and CASP3 expression in tested placentas. Furthermore, visfatin modulated INSR, ERK1/2, AKT, AMPKα, and STAT3 expression in JEG-3 and BeWo, and its anti-proliferative and pro-apoptotic effects occurred via mentioned factors. In conclusion, visfatin, by affecting the proliferation and apoptosis of human placenta cells, may be an important factor in the development and function of the organ.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"375-391"},"PeriodicalIF":3.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11833490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CSPG4 involvement in endometrial decidualization contributes to the pathogenesis of preeclampsia†. CSPG4参与子宫内膜蜕膜化是子痫前期的发病机制之一。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-02-14 DOI: 10.1093/biolre/ioae167

Tianying Zhang, Hua Li, Enhui Jiang, Liang Zhang, Lisheng Liu, Cong Zhang

{"title":"CSPG4 involvement in endometrial decidualization contributes to the pathogenesis of preeclampsia†.","authors":"Tianying Zhang, Hua Li, Enhui Jiang, Liang Zhang, Lisheng Liu, Cong Zhang","doi":"10.1093/biolre/ioae167","DOIUrl":"10.1093/biolre/ioae167","url":null,"abstract":"Preeclampsia (PE) is a condition of pregnancy in which symptoms of hypertension develop after 20 weeks of gestation. it can lead to placental dysfunction, maternal and perinatal mortality and morbidity. The incidence of PE is increasing, posing a serious threat to the lives of pregnant women and their unborn children. Currently, most of the research on the pathogenesis of PE has focused on placenta, However, maternal decidualization is the basis for placental formation and growth. Chondroitin sulfate proteoglycan 4 (CSPG4) is a transmembrane protein that plays a role in cell proliferation, invasion, and migration. However, its function during decidualization is not yet understood. In this study, we investigated the role of CSPG4 and found that its expression was significantly down-regulated in the decidual tissue of patients with severe PE compared to normal pregnant women. During artificially induced decidualization, CSPG4 expression was significantly increased. Knockdown of CSPG4 by small interfering RNA inhibited decidualization, which, in turn, inhibited the invasion of trophoblast cells. In both pseudopregnant and pregnant mice, endometrial stromal cells proliferated rapidly and Cspg4 expression increased during decidualization. Therefore, we believe that CSPG4 plays a crucial role in the process of decidualization. The defect in decidualization caused by abnormal CSPG4 expression could lead to insufficient trophoblast invasion, ultimately contributing to the occurrence of PE.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"361-374"},"PeriodicalIF":3.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: In vitro maturation of oocytes: what is already known? 更正：卵母细胞的体外成熟：什么是已知的？

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-02-14 DOI: 10.1093/biolre/ioae177

引用次数: 0

AURKA inhibits the decidualization of the eutopic endometrium in endometriosis through nuclear factor-κB p65†. AURKA通过NF-κB p65†抑制子宫内膜异位症中异位子宫内膜的蜕膜化。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-02-14 DOI: 10.1093/biolre/ioae176

Fangyuan Sun, Ting Yu, Ying Zhang, Xiaoyan Zhong, Dan Wang, Yuanyuan Li, Mengxue Wang, Shucai Zhang, Tingting Yang

{"title":"AURKA inhibits the decidualization of the eutopic endometrium in endometriosis through nuclear factor-κB p65†.","authors":"Fangyuan Sun, Ting Yu, Ying Zhang, Xiaoyan Zhong, Dan Wang, Yuanyuan Li, Mengxue Wang, Shucai Zhang, Tingting Yang","doi":"10.1093/biolre/ioae176","DOIUrl":"10.1093/biolre/ioae176","url":null,"abstract":"Endometriosis is an estrogen dependent disease, which is related to infertility. Decidualization is a prerequisite for successful implantation of human embryos, and endometriosis affects the occurrence of decidualization. However, the mechanism that affects decidualization in endometriosis is not fully understood. Here, we find that Aurora kinase A (AURKA) is upregulated in the eutopic endometrium of endometriosis. AURKA inhibits the decidualization of stromal cells in the eutopic endometrium of endometriosis. Furthermore, in animal experiments, AURKA promotes endometriosis and inhibits decidualization in mice with endometriosis, leading to decreased expression of decidualization markers, such as prolactin, insulin-like growth factor-binding protein-1, and desmin. Afterwards, we find that nuclear factor-κB (NF-κB) p65 is a new substrate of AURKA. AURKA interacts with p65 to promote its phosphorylation and nuclear translocation. Meanwhile, AURKA enhances the protein stability of p65 by prolonging its half-life. In summary, AURKA inhibits the decidualization of the eutopic endometrium in patients with endometriosis by regulating p65, which may provide new ideas for improving decidualization defect in patients with endometriosis.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"297-308"},"PeriodicalIF":3.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Attenuation of Ampullary Anoctamin 1 by the peritoneal fluid in rhesus macaques with spontaneous endometriosis†. 自发性子宫内膜异位症猕猴腹腔液对胰腺无克他命 1 的衰减作用

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-02-14 DOI: 10.1093/biolre/ioae173

Fangzhou Luo, Ov Slayden

{"title":"Attenuation of Ampullary Anoctamin 1 by the peritoneal fluid in rhesus macaques with spontaneous endometriosis†.","authors":"Fangzhou Luo, Ov Slayden","doi":"10.1093/biolre/ioae173","DOIUrl":"10.1093/biolre/ioae173","url":null,"abstract":"Altered peristaltic and ciliary dysfunction is a feature of females with endometriosis. To further explore this premise, we examined the ampulla of rhesus macaques (Macaca mulatta) with and without spontaneous endometriosis for the expression of adenylate kinase 7 (AK7), a mitochondrial-dwelling nucleotide converting enzyme with critical roles in cellular kinesis, forkhead protein box J1 (FOXJ1), a marker of cilia abundance, and Anoctamin 1 (ANO1) as a marker of both smooth muscle contraction and ciliogenesis. We further performed an in vitro experiment that treated ampullary segments with peritoneal fluid from animals with and without endometriosis. We report significantly downregulated expression of ANO1 in the ampulla of monkeys with endometriosis (in vivo), and in the ampullary segments exposed to peritoneal fluid of animals with endometriosis. We did not observe statistically significant differences in the expression of AK7 or FOXJ1 both in vivo and in vitro. This highlights potentially essential roles of ANO1 in the oviduct, the dampening of which may lead to a specific subtype of endometriosis-caused subfertility.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"286-296"},"PeriodicalIF":3.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11833491/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fertility and early embryonic development in a CD46-edited Gir heifer with reduced susceptibility to BVDV†. 对 BVDV 易感性降低的 CD46 编辑 Gir 小母牛的生育能力和早期胚胎发育。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2025-02-14 DOI: 10.1093/biolre/ioae169

Alexandria P Snider, Aspen M Workman, Michael P Heaton, Brian L Vander Ley, Alexandria C Krueger, Tad S Sonstegard

{"title":"Fertility and early embryonic development in a CD46-edited Gir heifer with reduced susceptibility to BVDV†.","authors":"Alexandria P Snider, Aspen M Workman, Michael P Heaton, Brian L Vander Ley, Alexandria C Krueger, Tad S Sonstegard","doi":"10.1093/biolre/ioae169","DOIUrl":"10.1093/biolre/ioae169","url":null,"abstract":"Bovine viral diarrhea virus (BVDV) infection during pregnancy is a significant contributor to reproductive failures in cattle. The bovine receptor for BVDV (CD46) was previously edited with a six amino acid substitution (G82QVLAL to A82LPTFS) and shown to have significantly reduced BVDV susceptibility in a Gir heifer calf. Since a role for CD46 has been proposed in mammalian fertilization, our objective was to assess the edited heifer's fertilization rates, early embryonic development, and germline transmission conformation of the edit. Cumulus oocyte complexes were collected from the edited heifer and unedited females, fertilized with semen from an unedited bull and cultured until the blastocyst stage. Ultrasound examinations and serum progesterone concentration were also monitored to confirm estrous cyclicity in the CD46-edited heifer. Estrous cyclicity was normal with visualization of a corpus luteum and elevated progesterone concentrations. Fertilization rates and blastocyst development were not different in oocytes from edited and unedited controls. Genome sequence analysis of blastocysts confirmed germline transmission of either edited allele from the heifer. Subsequently, the CD46-edited heifer was artificially inseminated with semen from an unedited Gir bull and fertility status was confirmed with a diagnosed conception at Day 35 of gestation. Thus, a six amino acid substitution in CD46 did not negatively affect fertilization of edited oocytes or early embryonic development when fertilized with semen from an unedited bull. An edited bull is still needed to similarly evaluate reproductive function of sperm cells carrying this CD46 edit.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"245-252"},"PeriodicalIF":3.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0