Biology of Reproduction最新文献_第7页

DNA methyltransferases in trophoblast differentiation: extant knowledge and emerging paradigms. DNA甲基转移酶在滋养细胞分化：现有的知识和新兴的范式。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-21 DOI: 10.1093/biolre/ioag203

Enoch Appiah Adu-Gyamfi

{"title":"DNA methyltransferases in trophoblast differentiation: extant knowledge and emerging paradigms.","authors":"Enoch Appiah Adu-Gyamfi","doi":"10.1093/biolre/ioag203","DOIUrl":"https://doi.org/10.1093/biolre/ioag203","url":null,"abstract":"Successful human pregnancy depends on the precise spatio-temporal orchestration of trophoblast lineages to establish a functional feto-maternal interface. While DNA methyltransferases (DNMTs) are recognized as core drivers of epigenetic programming, emerging evidence reveals that they are not merely passive transcriptional silencers, but also dynamic architectural coordinators of placental morphogenesis. This work presents the roles of maintenance (DNMT1) and de novo (DNMT3A, DNMT3B) methyltransferases in dictating the precise timing of trophoblast fate transitions. During trophoblast fusion, a coordinated downregulation of the DNMT machinery permits the localized demethylation and activation of vital fusogenic networks. Conversely, extravillous trophoblast invasion requires active DNMT-mediated hypermethylation to silence anti-invasive genes. Disruptions in these tightly synchronized epigenetic pulses trigger lineage-specific malfunctions that are associated with the pathogenesis of gestational disorders such as preeclampsia and fetal growth restriction. Despite these insights, fundamental knowledge gaps remain unaddressed. For instance, how DNMT expression and enzymatic assembly adapt to localized physical forces at the feto-maternal interface remains unknown. It is also unclear whether DNMTs exhibit non-canonical and non-enzymatic structural scaffolding properties during early implantation, and whether functional DNMT machinery is actively encapsulated within placenta-derived extracellular vesicles to cross the fetal blood-brain barrier and alter distant fetal neurodevelopment. Resolving these profound mechano-chemical, structural, and systemic knowledge gaps is essential to deciphering early human development and identifying novel therapeutic targets in feto-maternal medicine.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149011805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Acrosome Exocytosis as a Diagnostic Functional Test: a narrative review. 顶体胞吐作为一种诊断功能测试：叙述回顾。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-17 DOI: 10.1093/biolre/ioag196

Jorge Arturo Torres Juárez, Aída Guadalupe Díaz Martel, Ana Laura Gutiérrez Silva, Esperanza Mata Martínez, Haydé Vergara Castañeda, Claudia Treviño Santa Cruz, Ana Alicia Sánchez Tusie

{"title":"The Acrosome Exocytosis as a Diagnostic Functional Test: a narrative review.","authors":"Jorge Arturo Torres Juárez, Aída Guadalupe Díaz Martel, Ana Laura Gutiérrez Silva, Esperanza Mata Martínez, Haydé Vergara Castañeda, Claudia Treviño Santa Cruz, Ana Alicia Sánchez Tusie","doi":"10.1093/biolre/ioag196","DOIUrl":"https://doi.org/10.1093/biolre/ioag196","url":null,"abstract":"Infertility affects millions of couples worldwide, and male factors contribute to approximately 20-50% of infertility cases. Male infertility is typically evaluated with a semen analysis, which is a fundamental analysis to determine sperm quality. However, this analysis is limited. One of its most important deficiencies is the lack of functional sperm assessment. Because of this, semen analysis does not reveal information about the in vivo behavior of spermatozoa or their fertilization potential. This constitutes a concern because, in conventional semen analyses, 30% of infertile men display normal values in their semen analysis. In contrast, functional tests such as sperm-zona pellucida (ZP) binding assay and acrosome exocytosis (AE, also known as acrosome reaction) test predict male fertilization potential with greater accuracy. Here, we review functional tests and their potential to improve male infertility diagnostics and their predictive value for fertilization rates after IVF intervention. We also discuss the advantages of AE as a functional test to improve the diagnosis of male infertility in humans and other species, and its implementation in the clinic.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Segmentation of placental tissue using immunofluorescent staining and an artificial intelligence-based analysis workflow. 使用免疫荧光染色和基于人工智能的分析工作流程分割胎盘组织。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-17 DOI: 10.1093/biolre/ioag201

Rebecca Reif, Stephanie K Yanow, Denise G Hemmings

{"title":"Segmentation of placental tissue using immunofluorescent staining and an artificial intelligence-based analysis workflow.","authors":"Rebecca Reif, Stephanie K Yanow, Denise G Hemmings","doi":"10.1093/biolre/ioag201","DOIUrl":"https://doi.org/10.1093/biolre/ioag201","url":null,"abstract":"The placenta continuously remodels in response to maternal and fetal signals, with proteins dynamically regulated across placental regions. However, methods to evaluate proteins within these distinct regions are limited. To address this, we developed a placental tissue classifier to segment regions corresponding to the villous core, villous trophoblast (VT) and the intervillous space (IVS) using HALO AI imaging analysis. Tissue sections of biopsies from human term placentas embedded in OCT or paraffin (FFPE) were stained by immunofluorescence with antibodies to placental alkaline phosphatase (PLAP), syndecan-1 (SDC-1), vimentin or E-cadherin. The placental tissue classifier was trained using PLAP and DAPI staining with distinct image-feature patterns to distinguish VT from the villous core and the IVS on stained sections. The accuracy of HALO to measure area and intensity of staining in classified regions was demonstrated by staining for SDC-1 on VT and vimentin on stromal cells. As expected, SDC-1 staining was low in the villous core, and higher on VT than in the IVS, whereas vimentin staining was only detected in the villous core. By applying this analysis method to images collected by whole slide scanning microscopy, the area of staining was increased 245 times compared to a single field of view, which increases the probability of detecting pathological changes in different regions of the placenta. The advantages of using this validated classifier are the speed and accuracy of analysis across large tissue areas, the flexibility to detect other cell types, and to expand to single villi analysis.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a chemically defined cryopreservation solution for rat sperm and production of offspring by in vitro fertilization. 一种化学定义的大鼠精子冷冻保存液的研制和体外受精后代的产生。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-16 DOI: 10.1093/biolre/ioag200

Kohtaro Morita, Keiji Mochida, Ayumi Hasegawa, Shunya Ihashi, Kento Morita, Saki Wajima, Miho Kohara, Tadashi Sankai, Arata Honda, Kimiko Inoue, Atsuo Ogura, Masahide Asano

{"title":"Development of a chemically defined cryopreservation solution for rat sperm and production of offspring by in vitro fertilization.","authors":"Kohtaro Morita, Keiji Mochida, Ayumi Hasegawa, Shunya Ihashi, Kento Morita, Saki Wajima, Miho Kohara, Tadashi Sankai, Arata Honda, Kimiko Inoue, Atsuo Ogura, Masahide Asano","doi":"10.1093/biolre/ioag200","DOIUrl":"https://doi.org/10.1093/biolre/ioag200","url":null,"abstract":"In vitro fertilization (IVF) and artificial insemination (AI) using frozen-thawed rat sperm have been established to enable the production of viable offspring. However, the current standard cryopreservation solution, which contains chicken egg yolk and Orvus ES Paste (OEP; sodium lauryl sulfate and other undefined components), poses the risk of microbial contamination and limits the experimental reproducibility of IVF and AI. In this study, we developed a chemically defined cryopreservation solution that does not contain chicken egg yolk or OEP and evaluated its efficacy in IVF. After examining the effects of various cryoprotective agents on sperm motility, we found that OptiPrep (a chemically defined reagent) and ethylene glycol in a lactose solution best preserved sperm motility after freezing and thawing. In addition, sericin, a cell membrane protective agent, effectively reduced damage to the plasma and acrosomal membranes of the sperm head. Although the fertilization rate of the sperm cryopreserved in a chemically defined solution (71.4%) was significantly lower than that of fresh sperm (90.5%), fertilized oocytes were successfully obtained. Approximately 60% of the fertilized oocytes developed to the blastocyst stage in vitro. Furthermore, when two-cell embryos were transferred to the oviducts of pseudopregnant females, 36% developed into offspring. Taken together, our results suggest a new strategy for the cryopreservation of rat strains using a chemically defined sperm-freezing solution, enabling the safe and reproducible production of offspring.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148975591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-chain polyunsaturated fatty acids enhance the formation of rotifer dormant encysted embryos†. 长链多不饱和脂肪酸促进轮虫休眠囊胚的形成。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-14 DOI: 10.1093/biolre/ioag106

Tamar Ziv, Øystein Sæle, Patrick J Babin, Théo Mercé, Inna Khozin-Goldberg, Sagar Nayak, Yehuda G Assaraf, Esther Lubzens

{"title":"Long-chain polyunsaturated fatty acids enhance the formation of rotifer dormant encysted embryos†.","authors":"Tamar Ziv, Øystein Sæle, Patrick J Babin, Théo Mercé, Inna Khozin-Goldberg, Sagar Nayak, Yehuda G Assaraf, Esther Lubzens","doi":"10.1093/biolre/ioag106","DOIUrl":"10.1093/biolre/ioag106","url":null,"abstract":"This study uncovers the critical role of dietary n-3 polyunsaturated fatty acids (PUFAs) in the development of dormant embryos, known as resting eggs (REs), in the aquatic rotifer Brachionus plicatilis. REs contained PUFAs, and the maternal diet markedly influenced their levels. In this respect, supplementing PUFAs-deficient yeast with eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) in the diet increased RE production. A combination of EPA and DHA yielded the highest number of produced REs and reduced the percentage of damaged REs, without altering hatching rates. Proteome profiling investigated the impact of EPA and DHA on protein expression patterns. It identified 283 differentially expressed proteins out of 1954 and indicated distinct EPA and DHA functions. Differentially expressed proteins were linked to multiple functional pathways, suggesting nuclear receptor regulation. In vertebrates, EPA, DHA, and α-linolenic acid (ALA) interact with retinoid and peroxisome proliferator-activated receptors. These receptors emerged as promising regulators in PUFAs-treated rotifers. Consequently, the impact of ALA was also assessed. Remarkably, ALA enhanced reproductive rates, increased RE production, reduced the proportion of damaged REs, and improved hatching in a dose-dependent manner, even in the absence of EPA and DHA. These findings emphasize the essential roles of PUFAs in rotifer RE formation, a previously unexplored topic. The study stresses the need to investigate the roles of PUFAs during dormancy. This is important because global warming may reduce the availability of PUFAs, decrease the quantity of REs in egg banks, and affect the long-term viability of aquatic non-vertebrate populations.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"707-723"},"PeriodicalIF":3.2,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148004322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial uncoupler BAM15 attenuates cryopreservation-induced damage in human sperm by stabilizing mitochondrial homeostasis†. 线粒体解偶联剂BAM15通过稳定线粒体稳态减轻人类精子中低温保存引起的损伤。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-14 DOI: 10.1093/biolre/ioag123

Bei Chen, Cong Liu, Mengneng Xiong, Ling Xu, Jie Yao, Liangzi Guan, Zhifeng Sun, Qingzhen Xie, Yi Zhang, Yaqin Wang, Jin Luo

{"title":"Mitochondrial uncoupler BAM15 attenuates cryopreservation-induced damage in human sperm by stabilizing mitochondrial homeostasis†.","authors":"Bei Chen, Cong Liu, Mengneng Xiong, Ling Xu, Jie Yao, Liangzi Guan, Zhifeng Sun, Qingzhen Xie, Yi Zhang, Yaqin Wang, Jin Luo","doi":"10.1093/biolre/ioag123","DOIUrl":"10.1093/biolre/ioag123","url":null,"abstract":"Human sperm cryopreservation is essential for sperm banking and assisted reproduction, yet freeze-thaw stress promotes oxidative injury that reduces motility and damages the acrosome and nuclear DNA. Here, we tested whether the mitochondrial uncoupler BAM15 improves post-thaw human sperm quality and examined mechanisms linked to mitochondrial homeostasis. Ejaculates were cryopreserved using a standard protocol supplemented with graded concentrations of BAM15. After thawing, total and progressive motility and viability were assessed. Flow cytometry quantified the DNA fragmentation index and the proportion of high DNA stainability cells. Mitochondrial membrane potential, intracellular reactive oxygen species, and lipid peroxidation were measured to evaluate mitochondrial function and oxidative status. Ultrastructural preservation of the acrosome, plasma membrane, midpiece mitochondria, and flagellar axoneme was examined by transmission electron microscopy. Compared with untreated controls, BAM15 increased total and progressive motility and improved viability. BAM15 reduced DNA fragmentation and decreased high DNA stainability, indicating enhanced genomic integrity. Consistently, BAM15 improved mitochondrial membrane potential while suppressing intracellular reactive oxygen species and lipid peroxidation, supporting attenuation of freeze-thaw oxidative damage. Transmission electron microscopy further revealed more continuous acrosomal and plasma membranes, fewer swollen or vacuolated midpiece mitochondria, and improved preservation of axonemal architecture. Collectively, these findings identify BAM15 as a promising cryopreservation supplement that stabilizes mitochondrial homeostasis and improves the functional and structural quality of human sperm after thawing.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"777-788"},"PeriodicalIF":3.2,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148256724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic, environmental, and sex-specific programming of long-term health in Assisted Reproductive Technologies-conceived offspring†. art受孕后代长期健康的表观遗传、环境和性别特异性编程。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-14 DOI: 10.1093/biolre/ioag137

Eric A Rhon-Calderon, Cassidy N Hemphill, Marisa S Bartolomei

{"title":"Epigenetic, environmental, and sex-specific programming of long-term health in Assisted Reproductive Technologies-conceived offspring†.","authors":"Eric A Rhon-Calderon, Cassidy N Hemphill, Marisa S Bartolomei","doi":"10.1093/biolre/ioag137","DOIUrl":"10.1093/biolre/ioag137","url":null,"abstract":"Assisted Reproductive Technologies (ART) are non-coital methods of conception widely used to address infertility. These procedures include conventional in vitro fertilization (IVF), intracytoplasmic sperm injection, gamete and embryo cryopreservation, trophectoderm biopsy for pre-implantation genetic testing, and newer mitochondrial replacement techniques. Since the first IVF birth in 1978, the global use of ART has increased rapidly, contributing to more than 13 million births worldwide. Although ongoing technological advances have improved safety and increased pregnancy and live-birth rates, ART has also been associated with adverse placental and fetal outcomes, some of which persist into later life and, in certain cases, across generations. The mechanisms underlying these outcomes are not fully understood, but studies suggest that altered epigenetic reprogramming, environmental influences, and sex-specific factors are major contributors. In this review, we examine how long-term health outcomes in ART-conceived offspring are shaped by the interplay of epigenetic mechanisms, environmental influences, and sex-specific responses in both humans and mice.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"687-706"},"PeriodicalIF":3.2,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13578493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148410297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal obesity restricts fetal growth through placental energy failure and iron-associated oxidative stress in mice†. 小鼠中母体肥胖通过胎盘能量衰竭和铁相关氧化应激限制胎儿生长†。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-14 DOI: 10.1093/biolre/ioag131

Yeqianli Wo, Jiabing Mao, Zeou Wei, Ye Xu, Xiaohong Wang, Dadan Liu, Shusong Wu, Jianhua He, Martine Schroyen, Bao Yi, Liang Chen, Hongfu Zhang

{"title":"Maternal obesity restricts fetal growth through placental energy failure and iron-associated oxidative stress in mice†.","authors":"Yeqianli Wo, Jiabing Mao, Zeou Wei, Ye Xu, Xiaohong Wang, Dadan Liu, Shusong Wu, Jianhua He, Martine Schroyen, Bao Yi, Liang Chen, Hongfu Zhang","doi":"10.1093/biolre/ioag131","DOIUrl":"10.1093/biolre/ioag131","url":null,"abstract":"Maternal obesity compromises placental development and fetal growth, yet the mechanisms remain unknown. Here, we investigated the impact of high-fat diet (HFD) on placental energy homeostasis in mice. Female C57BL/6 mice were fed a control diet or an HFD, with a subset receiving FerroTerminator-1 (FOT1) to modulate metabolic dysfunction. At embryonic day 18.5, maternal metabolic status, fetal growth, placental morphology, energy metabolism, and oxidative stress were assessed. HFD-fed dams developed dyslipidemia and insulin resistance, and exhibited placental lipid accumulation and impaired labyrinth zone development, resulting in reduced fetal weight and altered fetal liver lipid and glycogen contents. The placenta showed mitochondrial damage, accompanied by reduced adenosine triphosphate (ATP) content, mitochondrial membrane potential, mitochondrial DNA copy number, and suppressed respiratory chain complex activities. Placental metabolomic profiling showed that HFD was associated with altered levels of metabolites in glycolysis and oxidative phosphorylation pathways. These alterations coincided with placental iron-associated oxidative stress, evidenced by elevated non-heme iron, lipid peroxidation products, upregulation of transferrin receptor and lysophosphatidylcholine acyltransferase 3, downregulation of ferritin heavy chain 1 and solute carrier family 40 member 1, a reduced glutathione to oxidized glutathione ratio, and impaired glutathione peroxidase 4 expression and activity. Meanwhile, FOT1 administration attenuated placental oxidative stress, partially restored ATP production and mitochondrial function, and improved fetal growth in HFD-fed mice. Collectively, maternal obesity was associated with placental iron-associated oxidative stress and bioenergetic impairment, which may contribute to reduced placental efficiency and fetal growth restriction.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"939-953"},"PeriodicalIF":3.2,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148683405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular matrix deficiency contributes to irregular uterine bleeding following progestin therapy for adenomyosis†. 细胞外基质缺乏导致孕激素治疗bb0后不规则子宫出血。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-14 DOI: 10.1093/biolre/ioag141

Xiaoye Ye, Ou Jin, Aolin Zheng, Nengyuan Lv, Jialu Guo, Jingjing Xiang, Hao Chen, Fengjuan Liu, Yuan Liu, Chunyu Jin, Yongfeng Wu, Jinyi Tong

{"title":"Extracellular matrix deficiency contributes to irregular uterine bleeding following progestin therapy for adenomyosis†.","authors":"Xiaoye Ye, Ou Jin, Aolin Zheng, Nengyuan Lv, Jialu Guo, Jingjing Xiang, Hao Chen, Fengjuan Liu, Yuan Liu, Chunyu Jin, Yongfeng Wu, Jinyi Tong","doi":"10.1093/biolre/ioag141","DOIUrl":"10.1093/biolre/ioag141","url":null,"abstract":"Adenomyosis is a common gynecological disorder that causes heavy menstrual bleeding and chronic pelvic pain. Progestin-based therapy is widely used for long-term management, but irregular uterine bleeding frequently occurs and severely affects patients' quality of life. The mechanisms underlying this side effect remain unclear. In this study, we performed proteomic sequencing on endometrial tissues from adenomyosis patients before progestin treatment. We found that patients who later developed irregular bleeding exhibited significantly reduced extracellular matrix and collagen levels. Cell experiments showed that matrix metalloproteinase inhibition increased collagen expression in patient-derived endometrial stromal cells. In a mouse model of progestin-induced uterine bleeding, both matrix metalloproteinase inhibition and intrauterine collagen supplementation effectively reduced bleeding. These findings indicate that insufficient extracellular matrix predisposes patients to bleeding during progestin therapy and provide potential strategies to prevent progestin-related irregular bleeding.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"750-761"},"PeriodicalIF":3.2,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148418318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic maintenance of male reproductive longevity: SIRT7 as a guardian of male germline genome integrity†. 男性生殖寿命的表观遗传维持：SIRT7作为男性生殖系基因组完整性的守护者。

IF 3.2 2区生物学

Biology of Reproduction Pub Date : 2026-09-14 DOI: 10.1093/biolre/ioag144

Leah E Simon, T Rajendra Kumar

引用次数: 0