Biology of Reproduction最新文献

{"title":"Loss of progesterone receptor in smooth muscle cells has no impact on oviductal contractions and preimplantation embryo development†.","authors":"Emily A McGlade, Jiude Mao, Kalli K Stephens, Cayce N Rose, John P Lydon, Wipawee Winuthayanon","doi":"10.1093/biolre/ioaf042","DOIUrl":"10.1093/biolre/ioaf042","url":null,"abstract":"","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"786-788"},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome transcriptome and DNA methylome analyses reveal molecular abnormalities during the oocyte-to-embryo transition in preimplantation embryos derived from prepubertal lamb oocytes†.","authors":"Qi Qi, Jiangyue Bian, Junjin Li, Kexiong Liu, Fengxiang Yan, Jian Hou","doi":"10.1093/biolre/ioaf045","DOIUrl":"10.1093/biolre/ioaf045","url":null,"abstract":"<p><p>The juvenile in vitro embryo transfer technology holds the potential to accelerate livestock breeding. However, its application is limited due to the weak in vitro development of oocytes and embryos from prepubertal lambs. To dissect the regulatory networks of gene expression of sheep embryos and identify the defects in gene expression in prepubertal lamb embryos during the oocyte-to-embryo transition, full-length RNA sequencing and whole-genome bisulfite sequencing based on trace cells were conducted on in vitro-derived embryos generated from adult sheep and prepubertal lamb oocytes. We found that the maternal transcript degradation occurred selectively in adult sheep embryos in multiple waves and was most completed until the morula stage. Major embryonic genome activation was found to occur at the morula stage. By comparing with the patterns of adult embryos, we observed incomplete maternal transcript degradation and abnormal embryonic genome activation in lamb embryos and analyzed their potential molecular mechanisms. Furthermore, we explored dynamic DNA methylation concerning the paternal and maternal genomes during the preimplantation development of sheep embryos, revealing the negative regulatory role of promoter DNA methylation on embryonic genome activation process. Lamb embryos generally displayed higher DNA methylation levels than adults, potentially repressing the embryonic genome activation gene expression, especially the genes associated with ribosomal and mitochondrial organization. We also found abnormalities in the methylation status of imprinted genes in lamb embryos. Our findings advance the understanding of sheep in vitro embryo development and offer insights for improving the juvenile in vitro embryo transfer technology in livestock.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"824-839"},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Duplex sequencing identifies unique characteristics of ENU-induced mutations in male mouse germ cells†.","authors":"Danielle P M LeBlanc, Gu Zhou, Andrew Williams, Matthew J Meier, Charles C Valentine, Jesse J Salk, Carole L Yauk, Francesco Marchetti","doi":"10.1093/biolre/ioaf029","DOIUrl":"10.1093/biolre/ioaf029","url":null,"abstract":"<p><p>Germ cell mutagenicity testing is increasingly required for chemical risk assessment. Duplex sequencing is rapidly gaining acceptance as a method to assess in vivo mutagenesis, and as a valid alternative to transgenic rodent mutation models such as the MutaMouse. We used a duplex sequencing panel of 20 genomic targets and the transgenic rodent assay to measure mutations in the germ cells of MutaMouse males exposed to 0, 1, 2, or 5 mg/kg N-ethyl-N-nitrosourea for 28 days. Germ cells from the seminiferous tubules were collected 28 days post-exposure. The transgenic rodent assay showed a significant increase in mutant frequencies at the high (P < 0.001) and medium (P = 0.01) N-ethyl-N-nitrosourea doses relative to controls, while duplex sequencing revealed a significant increase (P < 0.001) in N-ethyl-N-nitrosourea-induced mutations only at the high dose. Duplex sequencing mutation frequencies were lower in genic than in intergenic targets, suggesting a protective role for transcription-coupled repair. Interestingly, we observed several unique germ cell characteristics with respect to duplex sequencing data from rodent somatic tissues: 1) larger inter-animal variability in clonally expanded mutations that affects the ability to detect significant increases in mutation frequency; 2) a target on chromosome 2 showing much higher susceptibility to spontaneous and chemical-induced mutagenesis than other targets; and 3) a mutation spectrum consistent with that observed in the offspring of N-ethyl-N-nitrosourea-treated males but not with the spectrum in bone marrow of directly-exposed males. These results suggest that duplex sequencing is a promising approach for characterizing germ cell mutagenesis and that mutagenic mechanisms operating in germ cells differ from those in somatic tissues.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1015-1027"},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlorogenic acid alleviates deoxynivalenol-induced damage in porcine trophectoderm cells by regulating the PI3K/AKT signaling pathway†.","authors":"Chaohui Dai, Jinhua Cheng, Hui Li, Weimin Zhao, Bixia Li, Yanfeng Fu, Xuemin Wang, Chao Liao, Yanyu Chen, Junshu Yan","doi":"10.1093/biolre/ioaf110","DOIUrl":"https://doi.org/10.1093/biolre/ioaf110","url":null,"abstract":"<p><p>The proliferation and migration of porcine trophectoderm (pTr) cells are crucial processes during the early stages of embryo implantation in sows. The effects of deoxynivalenol (DON) and chlorogenic acid (CGA), a plant-derived compound, on pTr cells are currently unclear. In this study, pTr cells were treated with DON at different times (24, 48, and 72 hours) and different concentrations (0.5, 1, and 2 μg/mL) to construct a pathological model of DON-induced pTr cells by detecting the expression levels of genes related to cell proliferation, migration, and oxidative stress, as well as the cell viability and the cell migration ability. Subsequently, CGA intervention experiments revealed that CGA could promote the proliferation, migration, and antioxidant ability of pTr cells and alleviate the damage induced by DON in pTr cells. Finally, RNA-seq technology combined with experiments illustrated that CGA might alleviate the damage of DON-induced pTr cells by regulating the PI3K/AKT signaling pathway. In conclusion, this study explored the toxicological effect of DON and the alleviation effect of CGA on DON at the pTr cells level, which provided new insights and an experimental basis for using CGA to alleviate the reproductive toxicity induced by DON.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro inhibition of the CFTR ion channel in the Macaca mulatta cervix thickens cervical mucus†.","authors":"Rachel J Looney, Mackenzie Roberts, Matthew Markovetz, Rachelle Godiah, Shan Yao, Kirsti Golgotiu, Shuhao Wei, Chris Cellucci, Leo Han","doi":"10.1093/biolre/ioaf103","DOIUrl":"https://doi.org/10.1093/biolre/ioaf103","url":null,"abstract":"<p><p>Cervical mucus changes throughout the menstrual cycle in response to hormonal fluctuations, regulating access of sperm and pathogens to the reproductive tract. CFTR is an anion channel that plays a critical role in mediating epithelial mucus secretions. Primary endocervical cells obtained from rhesus macaques Macaca mulatta were cultured using conditional reprogramming and treated with vehicle controls or CFTR inhibitors. In order to measure changes in hydration and viscosity of secreted mucus, we adapted two airway mucus assays, airway surface liquid and particle-tracking microrheology, for our endocervical culture system. Endocervical cells treated with CFTR inhibitors demonstrated dehydrated, thicker mucus secretions compared to controls in both assay outputs. Our studies suggest that CFTR may be an important mediator of fertility changes and provide experimental evidence for the infertility phenotype seen in women with cystic fibrosis. Additionally, assays developed in these studies provide new endpoints for assessing cervical mucus changes in vitro.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: AURKA inhibits the decidualization of the eutopic endometrium in endometriosis through nuclear factor-κB p65.","authors":"","doi":"10.1093/biolre/ioaf106","DOIUrl":"https://doi.org/10.1093/biolre/ioaf106","url":null,"abstract":"","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges and strategies in the application of assisted reproductive technology (ART) in nonhuman primates (NHPs): review.","authors":"Jia Zhou, Liang Zhou, Ming Lan, Wei Ren, Zhengyi Wang","doi":"10.1093/biolre/ioaf109","DOIUrl":"https://doi.org/10.1093/biolre/ioaf109","url":null,"abstract":"<p><p>Nonhuman primates (NHPs) have become important models for biomedical research due to their similarities to humans in evolution, anatomy, and physiology. It is worth noting that NHPs have important clinical significance in the field of assisted reproductive technology (ART) research and are an important experimental basis for studying human reproductive physiology and pathogenic mechanisms. However, due to the differences in reproductive physiology between the two species, the application of human ART treatment strategies in NHPs often leads to different experimental results. Therefore, this review provides an analysis of the challenges encountered by NHPs in the application of ART and their possible solutions. This article discusses the technical status and difficulties of semen collection, quality assessment, egg collection, artificial insemination and gamete development in NHP. Based on the analysis of existing technologies, suggestions are put forward to optimize the assisted reproductive technology of NHPs, in order to improve the research model system of early embryo development of NHP. This study contributes to a deeper understanding of NHP and human embryo development, and provides some potential suggestions for optimizing NHP embryo culture and human infertility treatment. The optimization of NHP-ART has important scientific value and application prospects, and in-depth research can make more contributions to the progress of reproductive biology and clinical treatment. This review summarizes and analyzes the problems and strategies that deserve attention in some NHPs-ART technologies, so as to provide reference for researchers in related fields.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation and assessment of high-quality fat-tailed dunnart oocytes following superovulation in prepubertal animals†.","authors":"Jun Liu, Namdori Mtango, Emily L Scicluna, Sara Ord, Andrew J Pask","doi":"10.1093/biolre/ioaf104","DOIUrl":"https://doi.org/10.1093/biolre/ioaf104","url":null,"abstract":"<p><p>The fat-tailed dunnart, Sminthopsis crassicaudata, is a mouse-sized, polyovular, solitary dasyurid marsupial found in central and southern Australia. With the establishment of a chromosome-scale genome assembly, induced pluripotent stem cells, and targeted genetic editing, the dunnart is emerging as the laboratory marsupial model for comparative developmental, reproductive and conservation biology. The development of assisted reproductive technologies (ART) are critical to achieving these goals in this species. ART requires a large number of mature oocytes which are typically collected through stimulated and synchronised female reproductive cycles. While protocols for induced-ovulation or superovulation are standard in many placental mammals, there are no methods to date designed for marsupials. In the present study, prepubertal dunnarts were stimulated with pregnant mare serum gonadotrophin and human chronic gonadotrophin across 6 different treatment regimens. Our best regimen resulted in over 70% of prepubertal dunnarts ovulating with 82% normal oocytes. When the primed females were mated with stud males, 4-cell stage embryos were collected 48 h post-hCG administration. At around 96 h post-hCG, 50% (n = 8) and 78% (n = 9) of the embryos developed to blastocysts. Our results demonstrated successful stimulation of ovulation and mature oocyte collection in prepubertal dunnarts. Furthermore, we confirmed developmental competence of the induced ovulated oocytes through to at least the blastocyst stage. These findings represent the first robust hormonal regimen for predictable oocyte generation in any marsupial and will significantly contribute to the use of the dunnart in developmental and conservation biology.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astaxanthin promotes in vitro maturation of mouse oocytes by regulating mitochondrial functions and protein homeostasis.","authors":"Xinyi Yuan, Wanxia Zhong, Chuanxin Zhang, Xueqi Dong, Xiaoyu Yin, Tiantian Deng, Jinzhu Song, Jiawei Wang, Yuxin Xu, Hui Liu, Keliang Wu, Boyang Liu","doi":"10.1093/biolre/ioaf107","DOIUrl":"https://doi.org/10.1093/biolre/ioaf107","url":null,"abstract":"<p><p>The quality of mouse oocytes that are matured in vitro is much lower than the ones that are matured in vivo. This difference is mainly due to oxidative stress occurring in culture conditions. Astaxanthin, a marine carotenoid, is one of the most robust natural antioxidants that can effectively reduce oxidative stress. However, the role of astaxanthin in oocyte in vitro maturation (IVM) has not been reported. Here, we showed that the addition of astaxanthin successfully increased the maturation rate of mouse IVM oocytes. Single-cell transcriptome sequencing showed that the differential genes were mainly enriched to mitochondrial functions and protein homeostasis. By using micro-proteomics, we further showed that the mitochondrial activity was enhanced by astaxanthin addition. To reveal the developmental competence of astaxanthin-treated IVM oocytes and conquer the zona pellucida hardening, we applied zona pellucida laser optoperforation to assist the in vitro fertilization (IVF). We found that the 2-cell embryo rate was significantly increased in astaxanthin-treated oocytes. In summary, this study reveals that astaxanthin can efficiently promote mouse oocyte IVM and early embryonic development.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-organization and spatial localization of chromatin and epigenetic marks linked to nucleolar activity in porcine oocytes.","authors":"Merle Fenner, Michal Benc, Alexandra Rosenbaum Bartkova, Maria Pihl, Martine Chebrout, Martine Letheule, Frantisek Strejcek, Poul Hyttel, Jozef Laurincik, Kristine Freude, Amelié Bonnet-Garnier","doi":"10.1093/biolre/ioaf098","DOIUrl":"https://doi.org/10.1093/biolre/ioaf098","url":null,"abstract":"<p><p>Previous murine studies have established that large-scale chromatin modifications upon completion of oocyte growth are associated with nucleolar transcriptional silencing. These modifications seem essential both for completion of the oocyte's meiosis and subsequent embryonic developmental success. Investigating this putative interconnection between nucleolar transcriptional activity and spatial chromatin organization towards completion of oocyte growth in pigs, we scrutinized whether 3D chromatin organization and heterochromatin localization, along with epigenetic markers, could indicate oocyte quality and predict developmental competence of harvested porcine oocytes. Supravital brilliant-cresyl-blue (BCB) staining was used to classify porcine cumulus-oocyte-complexes (COCs) as fully grown (BCB+) or still growing (BCB-). Oocytes were analyzed via integrated 3D-immunofluorescence for nucleolar activity and heterochromatin markers, as well as 3D-DNA-FISH for specific heterochromatin sequences. Additionally, some oocytes were prepared for transmission electron microscopy (TEM). TEM revealed distinct ultrastructural differences between BCB+ and BCB- oocytes and validated BCB-staining as viable method for a rough assessment of oocyte developmental competence. Immunostaining identified all known germinal vesicle (GV) chromatin configurations (non-surrounded nucleolus (NSN), partially non-surrounded nucleolus (pNSN), partially surrounded nucleolus (pSN), surrounded nucleolus (SN)) and linked them to respective BCB categories. Nucleolar activity was detected only in NSN oocytes, predominantly from the BCB- group. Protein markers and FISH signals revealed significant 3D-organizational changes in chromatin between NSN and SN conformations, clustering around the nucleolus towards final oocyte maturation. These findings highlight an evident interconnection between nucleolar transcriptional silencing and specific 3D chromatin organization patterns, with changes in heterochromatin localization indicating completion of the oocytes' growth phase and marking higher competency for subsequent final maturation and embryonic development.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}