Biology of Reproduction最新文献

{"title":"The roles of AKT isoforms in decidualization and embryo implantation using a PGR-Cre mouse model.","authors":"Pascal Adam, François Fabi, Sophie Parent, Léa-Isabelle Renaud, Monique Cadrin, Eric Asselin","doi":"10.1093/biolre/ioaf062","DOIUrl":"https://doi.org/10.1093/biolre/ioaf062","url":null,"abstract":"<p><p>Implantation is a complex process requiring a prepared, receptive endometrium, reliant on synchronized decidualization of stromal cells. During this process, cell proliferation and apoptosis are tightly regulated by signaling factors, including the survival and proliferation PI3K/AKT pathway. The three AKT isoforms each play distinct physiological roles but their specific functions in endometrial cell survival and apoptosis remain unclear. We hypothesize that for successful implantation, each AKT isoform has distinct roles in the endometrium during decidualization, which varies throughout the process. To explore this, we developed a unique PGR-Cre tissue-specific mouse model with single and combined knockouts (KO) of each AKT isoform. Using artificial decidualization during pseudopregnancy and normal gestation, we investigated the specific activity of each AKT isoform and their downstream targets to assess the role of AKT pathway. Our results showed that the AKT1-2 KO genotype failed to decidualize during pseudopregnancy and exhibited a reduced number of implantation sites. Interestingly, AKT3 was hyperphosphorylated in the AKT1-2 KO mice and emerged as the primary isoform active throughout decidualization, specifically signaling through GSK3B. This study suggests distinct yet partially redundant roles for AKT1 and AKT2 during decidualization and embryo implantation. We propose that the AKT pathway plays significant role in fertility, and a deeper understanding of its involvement in decidualization could lead to improved strategies for addressing fertility issues. These findings highlight the importance of AKT activity in the cellular and molecular regulation of mouse fertility.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic targeting of the tryptophan-kynurenine Axis for HTR-8/SVneo trophoblast proliferation and migration in unexplained recurrent spontaneous abortion.","authors":"Pingping Jin, Xinyi Lu, Lu Wang, Yan Chen, Lan Yang, Yongxiang Yin, Ye Shen, Xinxin Ni, Daozhen Chen, Yun Zhang, Yu Chen","doi":"10.1093/biolre/ioaf040","DOIUrl":"https://doi.org/10.1093/biolre/ioaf040","url":null,"abstract":"<p><strong>Introduction: </strong>Recurrent spontaneous abortion (RSA) is associated with maternal-fetal interface dysfunction, particularly abnormal trophoblast invasion and proliferation. However, our understanding of the cause of RSA remains limited.</p><p><strong>Methods: </strong>Plasma Trp and Kyn levels were measured in two groups using ELISA. Immunofluorescence and western blot analyses were employed to evaluate the expression of IDO1, VEGFA, and proteins associated with epithelial-mesenchymal transition (EMT) in villous and decidual tissues from patients with recurrent spontaneous abortion (RSA). The effects of Tryptophan (Trp) and IDO1-driven Trp-Kynurenine (Kyn) metabolism on trophoblast proliferation, migration, EMT, and angiogenesis were investigated in the HTR-8/SVneo cell line using wound healing, transwell migration, quantitative real-time PCR (RT-qPCR), Western blotting, and tube formation assays. RNA sequencing identified differentially expressed genes in cells treated with 500 μM exogenous L-Trp.</p><p><strong>Results: </strong>RSA patients exhibited elevated plasma Trp levels and significantly reduced Kyn levels, indicating decreased IDO1 activity (as assessed by the Kyn/Trp ratio) compared to controls. IDO1, EMT-related proteins, and VEGFA were downregulated in RSA patient tissues. In vitro, L-Trp enhanced trophoblast migration, invasion, EMT, and microvasculature formation via IDO1 activation. The reduced functional capabilities induced by the IDO1 antagonist 1-MT (500 μM) were rescued by Kyn (300 μM). RNA sequencing revealed that L-Trp upregulation modulates trophoblast gene expression and functional pathways associated with amino acid metabolism, angiogenesis, and vasculature development.</p><p><strong>Discussion: </strong>Our study reveals a novel molecular mechanism by which Trp metabolism regulates HTR-8 cell function, suggesting that modulating IDO1 activity may represent a therapeutic strategy to improve trophoblast function and pregnancy outcomes in RSA.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HMGB1 promotes immune abnormalities in preeclampsia by recruiting monocyte/decidual macrophages and inducing M1 polarization.","authors":"Xixi Deng, Xueqi Li, Guiqiong Huang, Jiani Zhang, Tingting Xu, Ying Feng, Xiaodong Wang","doi":"10.1093/biolre/ioaf061","DOIUrl":"https://doi.org/10.1093/biolre/ioaf061","url":null,"abstract":"<p><p>Preeclampsia (PE) is a severe pregnancy complication associated with immune imbalance and placental hypoxia stress. Decidua macrophages (dMφ) are essential at maternal fetal interface, which is abnormally activated and excessively transformed to the M1-phenotype in PE. High mobility group box 1(HMGB1), released from necrotic cells after injury, accumulates in the placenta and peripheral blood of patients with PE. Therefore, this study aims to investigate the interaction between macrophages and trophoblasts, exploring how HMGB1 affects macrophage functions and its potential involvement in the pathophysiology of PE. Decidua tissue was obtained from 11 women with severe pre-eclampsia (sPE), 13 women with pre-eclampsia (PE), and 13 women with normal pregnancies. HMGB1 levels in decidua were evaluated by immunohistochemistry (IHC), western blot, and qPCR. Additionally, primary dMφ and peripheral blood monocytes (pMo) were isolated to develop a co-culture model simulating maternal fetal interface cell model of PE. Flow cytometric analysis and in vitro cell migration assay investigated the interaction between macrophages and HMGB1. This study identified elevated HMGB expression in PE patients. HMGB1 expressed widely in trophoblast, decidual stromal cells, and the extracellular matrix. Furthermore, hypoxia induced trophoblast to express and secrete HMGB1, promoting pMo and dMφ migration. Additionally, HMGB1 recruited monocyte-induced macrophages (pMφ) and modulated M1 macrophage polarization. HMGB1 is increased at the maternal-fetal interface of PE, which recruits monocytes and macrophages and induces their polarization to M1. This study offers insights into the suppressive effects of the crosstalk between dMφ and trophoblasts at the maternal-fetal interface, lending credence to macrophage-targeted interventions of PE as a potential therapeutic strategy.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANXA1 Inhibits Trophoblast Ferroptosis in Preeclampsia by Downregulating KISS1.","authors":"Yuzhu Rao, Shiming Tan, Jingjing Wang, Jingqiu Jia, Zemin Cai, Chunyan Wu, Peng Wu, Zuo Wang","doi":"10.1093/biolre/ioaf060","DOIUrl":"https://doi.org/10.1093/biolre/ioaf060","url":null,"abstract":"<p><p>Preeclampsia (PE) is a significant hypertensive disorder associated with pregnancy, impacting the health of women and children globally. It stands as one of the primary contributors to elevated morbidity and mortality rates among pregnant individuals and neonates. Recent investigations indicate a significant potential association between ferroptosis and PE. Annexin A1 (ANXA1) serves as an endogenous inhibitor of inflammation, capable of being activated by glucocorticoids, ischemia-reperfusion events, inflammatory processes, or oxidative stress. Ac2-26 is a synthetic peptide derived from the N-terminal 26 amino acids of the ANXA1 protein and retains its anti-inflammatory properties. Nevertheless, the precise regulatory mechanisms underlying ANXA1's role in PE remain to be fully elucidated. In this study, we first revealed that the increase in ferroptosis in preeclamptic placentas is accompanied by a downregulation of ANXA1 expression. Next, we established a PE-like mouse model and confirmed the presence of ferroptosis in the placentas of these mice. Ac2-26 treatment reduced placental ferroptosis and improved adverse pregnancy outcomes. Additionally, we demonstrated that targeting ANXA1 (Ac2-26) alleviates RSL3-induced trophoblast dysfunction and inhibits its promotion of intracellular lipid peroxidation. Subsequent mechanistic investigations have demonstrated that the elevation of KISS1 levels is intricately associated with ferroptosis and PE, while ANXA1 (Ac2-26) serves to inhibit KISS1 expression, thereby ultimately mitigating ferroptosis. In summary, this study presents the novel finding that elevated levels of KISS1 during pregnancy promote ferroptosis, a process that is mitigated by ANXA1 (Ac2-26) through the downregulation of KISS1 expression, thereby alleviating PE. This discovery offers a promising therapeutic strategy targeting the ferroptosis signaling pathway in PE.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental Programming: Mechanisms of Early Exposure to Real-Life Chemicals in Biosolids on Offspring Ovarian Dynamics†.","authors":"Yiran Zhou, Katherine M Halloran, Michelle Bellingham, Richard G Lea, Neil P Evans, Kevin D Sinclair, Peter Smith, Vasantha Padmanabhan","doi":"10.1093/biolre/ioaf053","DOIUrl":"https://doi.org/10.1093/biolre/ioaf053","url":null,"abstract":"<p><p>Female reproductive capacity is shaped by ovarian reserve and patterns of follicle development. Ovarian reserve depletion occurs by follicle activation and atresia, which can be affected by environmental chemicals (ECs). Because humans are simultaneously exposed to hundreds of ECs, real-life exposure models are essential to assess patterns of atresia after EC exposure. Previous findings demonstrate maternal preconceptional and gestational ECs exposure via biosolids-treated pasture (BTP) increases activation rate and reduces primordial follicle pool in juveniles, but not adults. We hypothesized that this shift involves changes in death and proliferative pathways that impact follicle atresia from juvenile to adult life. Ovaries were collected from juvenile (9.5 weeks) and adult (2.5 years) offspring from ewes grazed on either BTP or inorganic fertilizer-treated pasture (Control). Follicular atresia was assessed through morphological characteristics and molecular death pathways, including expression of markers for apoptosis (CASP3), autophagy (LC3), and ferroptosis (GPX4) and proliferation (Ki67). There were higher levels of apoptosis and autophagy, and lower proliferation, in juvenile BTP offspring compared to controls. In adult BTP offspring, apoptosis and proliferation did not differ, autophagy was lower, and ferroptosis was higher compared to controls. Apoptosis was lower and ferroptosis higher in adults than in juveniles, regardless of treatment. Adult BTP offspring had lower autophagy and similar proliferation levels to juvenile BTP. These findings suggest that lower autophagy and lack of decrease in proliferation contribute to normalization of activation rate and ovarian pool in BTP adults and supportive of lasting impacts of gestational EC exposure on offspring follicular health.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishing the baseline: understanding follicle activation morphometrics in multiple mammalian species.","authors":"Hana Kubo, Christopher J McCauley, Mary B Zelinski, Monica M Laronda","doi":"10.1093/biolre/ioaf059","DOIUrl":"https://doi.org/10.1093/biolre/ioaf059","url":null,"abstract":"<p><p>Folliculogenesis encompasses many stages as the somatic granulosa and theca cells support oocytes through growth and maturation. A novel follicle stage, between primordial and transitional stages, was identified in mice and defined as \"zip\". Like all other follicle stages, the \"zip\" stage is characterized by its granulosa cell morphology. The \"wedge\" GC morphology in zip follicles is predicted to be the first granulosa cell division, marking the transition from squamous to cuboidal morphology. Here, zip and transitional stages were identified in histological sections of porcine, bovine, rhesus monkey, and human ovaries. Several growth dynamics characterized at these follicle stages were conserved between species. Oocyte diameter and area increased between the primordial and transitional stages in the porcine ovary and between the primordial and primary stages in the rhesus monkey ovary but appeared unchanged in bovine and human ovaries. In all species except for pigs, granulosa cell number and height increased at stages earlier than observed changes in the oocyte. Furthermore, there were differences in the percentage of zip and transitional follicle stages present in the cortical region across species. This implies that there may be species-dependent activation and growth mechanisms that require further study. The parameters defined here for identifying and characterizing the zip and transitional follicle stages across species can act as a tool for measuring factors that perturb or induce primordial follicle activation or effect follicle morphometric parameters in support of future innovations for fertility preservation and restoration.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delayed Blastocyst Development is Associated with Altered Metabolism and Proteome in Male and Female Bovine Embryos.","authors":"Kyle J Fresa, Ming-Hao Cheng, Keira Y Larson, Alexandra A Crook, Anthony J Saviola, Raul A Gonzalez-Castro, Thomas W Chen, Elaine M Carnevale","doi":"10.1093/biolre/ioaf058","DOIUrl":"https://doi.org/10.1093/biolre/ioaf058","url":null,"abstract":"<p><p>Developmentally delayed embryos are associated with reduced implantation potential and live birth rates; however, inherent causes of delayed development are not well understood. Metabolism during preimplantation development is responsible for the production of energy and biosynthetic material to support growth, and disturbances to these pathways can reduce embryo viability. The present study utilized electrochemical microsensors to determine differences in rates for oxygen consumption, extracellular acidification, and hydrogen peroxide production between normal and slow-growing, male and female bovine blastocysts. In addition, pooled samples of blastocysts were subjected to proteomic analysis to determine differences in the abundance of proteins associated with metabolism between the sexes and developmental timing status. In comparison to blastocysts developing over a normal timespan, blastocysts forming 1 to 2 days later had a higher oxygen consumption rate, differences in abundance of electron transport complex proteins, and reduced abundance of biosynthetic enzymes when compared to blastocysts developing during a normal timeline. Embryo sex resulted in unique differences in metabolic enzyme abundance with potentially different contributions to delayed development. In addition, male and female blastocysts had differential protein abundances indicating differences in metabolic pathway activity. Therefore, embryos that took longer to reach the blastocyst stage of development appeared to have an imbalance between energy production and biosynthetic activity, which could differentially impact male and female embryos.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastics and Impaired Male Reproductive Health - Exploring Biological Pathways of Harm: A Narrative Review.","authors":"Naina Kumar, Mishu Mangla","doi":"10.1093/biolre/ioaf054","DOIUrl":"https://doi.org/10.1093/biolre/ioaf054","url":null,"abstract":"<p><strong>Introduction: </strong>Microplastics (MPs), pervasive environmental pollutants, have emerged as significant health hazards with growing evidence linking them to impaired male reproductive health. MPs can enter the human body through ingestion, inhalation, and dermal absorption, and once internalized, can induce oxidative stress, inflammation, endocrine disruption, and cellular damage leading to impaired male reproductive health. The present narrative review explores the biological pathways through which MPs impair male reproductive health, focusing on their direct and systemic effects.</p><p><strong>Methodology: </strong>A comprehensive literature search spanning up to February 2025 was conducted across electronic databases, including PubMed, Scopus, Web of Science, and Google Scholar. Search terms such as \"microplastic exposure,\" \"male infertility,\" \"male reproductive health\", \"oxidative stress,\" \"endocrine disruption,\" \"spermatogenesis,\" \"inflammation,\" and \"reproductive toxicity\" were employed to identify relevant studies published in peer-reviewed journals, books, and reputable conference proceedings. Inclusion criteria were limited to articles written in English that focused on the biological pathways linking MP exposure to impaired male reproductive health. Priority was given to review articles, original research papers, and meta-analyses. Extracted information was systematically organized to provide a narrative synthesis.</p><p><strong>Conclusion: </strong>Current evidence suggests that MPs may impair male reproductive health through mechanisms like oxidative stress, hormonal disruption, inflammation, and cellular damage. However, the lack of human studies highlights the urgent need for robust research to clarify their impact on human male infertility. Furthermore, this review underscores the necessity for continued research to elucidate molecular mechanisms, inform preventative strategies, and guide regulatory policies addressing MP pollution and its health implications.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverse roles of stress-responsive RNP granules in oogenesis and infertility.","authors":"M Rebecca Glineburg, Carolee Nguyen","doi":"10.1093/biolre/ioaf057","DOIUrl":"https://doi.org/10.1093/biolre/ioaf057","url":null,"abstract":"<p><p>Effectively responding to cellular stress (e.g. nutrient deprivation, oxidative stress) is essential for cell and organismal survival. A protective mechanism is especially critical in developing oocytes, where a prolonged quiescent state and the inability to divide render oocytes highly susceptible to accumulating stress that can result in cell death if unaddressed. Despite the common view that stress granules are the primary stress-responsive ribonucleoprotein (RNP) granule, accumulating evidence shows that in ovaries, other RNP granules also uniquely mediate gene regulation in response to stress. Here we review recent insights into RNP granule dynamics and RNP granule protein function during stress in the context of oogenesis among both invertebrates and vertebrates, with an emphasis on insights from Drosophila and mice. We also discuss roles for stress-responsive RNPs in maintaining stem cell populations, and complicating fertility treatments. By exploring how stress-induced RNP dynamics can impact oogenesis, both positively and negatively, we can better understand how stress contributes to reduced fecundity and infertility. We conclude by offering key research questions that can drive the next generation of insights.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure of Bovine Granulosa Cells to Lipopolysaccharide Reduces Progesterone Secretion During Luteinization.","authors":"Zachary K Seekford, Stephanie E Wohlgemuth, I Martin Sheldon, John J Bromfield","doi":"10.1093/biolre/ioaf055","DOIUrl":"https://doi.org/10.1093/biolre/ioaf055","url":null,"abstract":"<p><p>Uterine disease reduces fertility in dairy cows and is caused by pathogenic bacteria. During disease, lipopolysaccharide (LPS) accumulates in follicular fluid and triggers granulosa cell inflammation via the Toll-like receptor 4 pathway. Follicle growth and plasma estradiol are reduced in cows with uterine disease, and treatment of bovine granulosa cells with LPS reduces CYP19A1 expression and estradiol synthesis. It is unclear if the effects of LPS on the steroidogenic capacity of granulosa cells persist in cells during luteinization. We hypothesized that acute exposure of granulosa cells to LPS would alter progesterone synthesis during luteinization. Here, we demonstrate that acute exposure of granulosa cells to LPS reduces progesterone synthesis during a 9-day period of luteinization after LPS treatment. We show that exposure of granulosa cells to LPS does not alter the gene expression of STAR, HSD3B1 or CYP11A1, or cellular respiration during luteinization. However, acute exposure of granulosa cells to LPS reduces the abundance of intracellular lipid, mitochondria density and cholesterol uptake during luteinization, suggesting a potential mechanism of altered steroidogenesis after acute inflammation. Collectively, these findings show that exposure of granulosa cells to LPS reduces progesterone synthesis during luteinization which is associated with altered lipid droplets and mitochondria accumulation required for steroidogenesis. Perturbations to granulosa cell physiology during uterine disease may have prolonged effects on ovarian function that contribute to reduced fertility of cows. Understanding the effects of uterine disease on corpus luteum function after disease resolution can help explain disease associated subfertility in cattle.</p>","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}