Biology of Reproduction最新文献_第3页

High-Resolution Untargeted Metabolomics Reveals Altered Lysophosphatidylcholine, Palmitoleic Acid, Triglycerides and Isopulegone Caffeate Associated with Ovarian Injury in BTBR ob/ob Mice†. 高分辨率非靶向代谢组学揭示BTBR ob/ob小鼠卵巢损伤与溶血磷脂酰胆碱、棕榈油酸、甘油三酯和咖啡酸异戊二酮改变有关

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-14 DOI: 10.1093/biolre/ioag075

Yan Rong, Jiyun Chang, Zhuo Chen, Xinduo Zhang, Nan Sun, Jiaqi Liu, Yunxia Jiang, Hong Shen, Yan Chen, Zhiguo Li

{"title":"High-Resolution Untargeted Metabolomics Reveals Altered Lysophosphatidylcholine, Palmitoleic Acid, Triglycerides and Isopulegone Caffeate Associated with Ovarian Injury in BTBR ob/ob Mice†.","authors":"Yan Rong, Jiyun Chang, Zhuo Chen, Xinduo Zhang, Nan Sun, Jiaqi Liu, Yunxia Jiang, Hong Shen, Yan Chen, Zhiguo Li","doi":"10.1093/biolre/ioag075","DOIUrl":"https://doi.org/10.1093/biolre/ioag075","url":null,"abstract":"Diabetes mellitus adversely affects female reproductive health, leading to ovarian dysfunction and infertility. In this study, we investigated metabolic alterations in the ovaries of BTBR ob/ob mice, a well-established type 2 diabetes mellitus model, using high-resolution untargeted metabolomics combined with ion mobility spectrometry. Our analysis identified significant changes in key lipid metabolites, including polyunsaturated lysophosphatidylcholine, palmitoleic acid, triglyceride and isopulegone caffeate, which are closely linked to inflammatory and oxidative stress pathways. Notably, the decrease in anti-inflammatory polyunsaturated lysophosphatidylcholines and antioxidant isopulegone caffeate, along with the increase in pro-inflammatory palmitoleic acid and triglycerides, is accompanied by reduced interleukin-6 levels and elevated monocyte chemoattractant protein-1 expression. This indicates that the ovarian microenvironment has been disrupted and shifted toward inflammation. In addition, the increase in 4-hydroxynonenal, a marker of oxidative damage, further confirms that the vicious cycle of inflammation and oxidative stress leads to ovarian injury. These findings reveal the key metabolic disorders underlying diabetes-induced ovarian dysfunction, suggesting that polyunsaturated lysophosphatidylcholine, palmitoleic acid, triglycerides and isopulegone caffeate may be potential metabolic regulators and therapeutic targets in diabetic ovarian injury. However, their exact roles and mechanisms still need to be clarified through further functional studies.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The FSH/FSHR axis in reproductive biology and oncogenesis: mechanisms and emerging targeted therapies†. 生殖生物学和肿瘤发生中的FSH/FSHR轴：机制和新兴的靶向治疗。

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-13 DOI: 10.1093/biolre/ioaf287

Zhikun Wang, Zhihao Deng, Yiran Tian, Enliang Li, Qingping Yang

引用次数: 0

Dynamic spiral artery remodeling in early human pregnancy: an analysis of specimens collected with a standardized protocol†. 动态螺旋动脉重构在早期人类妊娠：标本收集与标准化协议分析†。

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-13 DOI: 10.1093/biolre/ioaf279

Shenglong Ye, Yeling Ma, Wenlong Li, Linjing Qi, Xiao Fang, Xin Yu, Duo Yu, Xiaoye Wang, Dong-Bao Chen, Yan-Ling Wang

{"title":"Dynamic spiral artery remodeling in early human pregnancy: an analysis of specimens collected with a standardized protocol†.","authors":"Shenglong Ye, Yeling Ma, Wenlong Li, Linjing Qi, Xiao Fang, Xin Yu, Duo Yu, Xiaoye Wang, Dong-Bao Chen, Yan-Ling Wang","doi":"10.1093/biolre/ioaf279","DOIUrl":"10.1093/biolre/ioaf279","url":null,"abstract":"Human uterine spiral artery remodeling (SAR) is a tightly regulated process involving complex interactions between interstitial and endovascular extravillous trophoblasts (iEVTs and enEVTs) and diverse maternal decidual cell populations. However, the intrinsic spatiotemporal dynamics of SAR in human placentation remain poorly understood, largely due to the limited availability of high-quality maternal-fetal interface specimens. Electively terminated early pregnancies offer a valuable resource for studying SAR in situ, yet inconsistent methods for distinguishing fragmented villous and decidual tissues have hindered reproducibility and interpretation. Herein we present a standardized protocol for the classification and characterization of high-quality maternal-fetal interface specimens from elective terminations by integrating stereomicroscopic evaluation with confirmation by immunohistochemistry and immunofluorescence microscopy. Combined with multiplex immunofluorescence imaging with cell-type-specific markers, this approach enabled precise spatial mapping and quantification of key morphological and cellular events in SAR from gestational weeks 5-10. Our analyses reveal that SAR initiates as early as week 5 with extraluminal recruitment of natural killer (NK) cells, followed by the formation of tightly packed EVT plugs within the lumens of spiral arteries in the decidua compacta; these plugs progressively extend deeper into the vessels and gradually loosen as gestation progresses. Notably, enEVTs appear to acquire NK cell-like phenotypes that may facilitate the displacement of endothelial and smooth muscle cells, promoting progressive vessel dilation. In summary, we provide a robust and reproducible method for assessing physiological SAR in early human pregnancy, promoting the adoption of our methodology in future studies of pathological SAR and related pregnancy disorders.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1445-1457"},"PeriodicalIF":3.0,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13079445/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative proteomic analysis of the zebrafish (Danio rerio) perivitelline fluid; identifying putative cortical alveoli-associated proteins. 斑马鱼卵泡周围液的比较蛋白质组学分析鉴定假定的皮层肺泡相关蛋白。

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-13 DOI: 10.1093/biolre/ioag012

Blake A Lewis, Karen L Reader, Michael W Pankhurst, Caroline W Beck, Mark P Lokman

{"title":"Comparative proteomic analysis of the zebrafish (Danio rerio) perivitelline fluid; identifying putative cortical alveoli-associated proteins.","authors":"Blake A Lewis, Karen L Reader, Michael W Pankhurst, Caroline W Beck, Mark P Lokman","doi":"10.1093/biolre/ioag012","DOIUrl":"10.1093/biolre/ioag012","url":null,"abstract":"Upon fertilization, the egg must have the resources to facilitate successful fertilization and regulate zygotic growth prior to the activation of the embryonic genome. A key biomarker of egg activation is the synchronized release of cortical alveoli (CA) from the egg's cortex into the perivitelline space. Teleost CA are primarily associated with their highly conserved roles in polyspermy prevention. However, several CA-associated proteins with functions related to innate immunity have been isolated. While there is growing evidence that teleost CA have extended biological roles outside of polyspermy prevention, little work has been done toward developing an understanding of their general proteomic composition. Therefore, the aim of this study was to identify proteins which were proportionally over-represented in the zebrafish (Danio rerio) perivitelline fluid (PVF) directly following CA exocytosis to identify a candidate list of CA-associated proteins. This study utilized a novel technique for PVF extraction from water-activated eggs shortly following CA exocytosis which negated the use of fixatives, thereby increasing PVF sample integrity for downstream mass-spectrometry analysis. Moreover, this study provides the first comparative proteomic analysis of the PVF relative to the yolk and cytoplasm of the zebrafish egg. As a result, 44 proteins were identified which were proportionally over-represented in the PVF, relative to the yolk and cytosol, of the water-activated unfertilized egg. The resulting PVF proteome was comprised of proteins associated with functions in carbohydrate binding and peptidase regulation. Many of these proteins are compelling candidates for being CA-derived and have been previously implicated in innate immunity.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1312-1323"},"PeriodicalIF":3.0,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13079451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146084069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developments on mammalian oocyte maturation using ZHB112-113, a novel long-acting human FSH and LH recombinant fusion protein†. 新型长效人卵泡刺激素与LH重组融合蛋白ZHB112-113在哺乳动物卵母细胞成熟中的研究进展

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-13 DOI: 10.1093/biolre/ioag004

Puyao Zhang, Chennan Ge, Chenyang Jiang, Yu Zhuang, Mengqian Zhu, Min Chen, Xuning Ding, Bruce Yong Ma

引用次数: 0

RTCB is essential for early mouse embryogenesis. RTCB对小鼠早期胚胎发生至关重要。

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-13 DOI: 10.1093/biolre/ioag008

Yu-Qi Chen, Mei He, Ran Li, Hai-Xia Hao, Chao Kong, Rui-Tao Zhang, Shu-Lei Liu, Pei-Yan Liu, Hua Ni, Cheng-Qiang He, Nai-Zheng Ding

{"title":"RTCB is essential for early mouse embryogenesis.","authors":"Yu-Qi Chen, Mei He, Ran Li, Hai-Xia Hao, Chao Kong, Rui-Tao Zhang, Shu-Lei Liu, Pei-Yan Liu, Hua Ni, Cheng-Qiang He, Nai-Zheng Ding","doi":"10.1093/biolre/ioag008","DOIUrl":"10.1093/biolre/ioag008","url":null,"abstract":"RTCB (RNA 2',3'-cyclic phosphate and 5'-OH ligase) is a unique 3'-5' RNA ligase with diverse physiological functions in metazoans. To further explore the role of RTCB in reproduction, we generated a Rtcb conditional knockout mouse model using the Ddx4-Cre system. The complete absence of viable Rtcb-/- offspring indicated embryonic lethality. Integrated analyses of histology, immunohistochemistry, quantitative polymerase chain reaction, and published single-cell RNA sequencing (scRNA-seq) data revealed that Rtcb is expressed throughout embryogenesis, with pronounced upregulation during gastrulation, and that Rtcb knockout resulted in gastrulation failure around embryonic day 6.5 (E6.5), which was accompanied by decreased proliferation and increased apoptosis of embryonic cells. Notably, NUSAP1 (nucleolar and spindle-associated protein 1) is vital for gastrulation as it participates in cell division. In NIH 3 T3 cells, knockdown of Rtcb led to destabilization of Nusap1 mRNA, suggesting that NUSAP1 might function downstream of RTCB. Moreover, Rtcb is a target gene of YY1 (Yin-Yang-1), a transcription factor crucial for gastrulation. Expression profiling through scRNA-seq revealed spatiotemporal coordination among Yy1, Rtcb, and Nusap1 between E3.5 and E7.5. Collectively, our findings demonstrate that RTCB is essential for early mouse embryogenesis and propose the presence of a YY1-RTCB-NUSAP1 axis that maintains proper cell proliferation for successful gastrulation.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1199-1209"},"PeriodicalIF":3.0,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gestational intermittent hypoxia programs pregnancy-specific salt-sensitive postpartum hypertension and vascular dysfunction via disruption of NO/cGMP/PKG pathway†. 通过破坏NO/cGMP/PKG通路的妊娠期盐敏感性产后高血压和血管功能障碍

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-13 DOI: 10.1093/biolre/ioaf289

Ruolin Song, Pankaj Yadav, Alissa Hofmann, Jay S Mishra, Sathish Kumar

{"title":"Gestational intermittent hypoxia programs pregnancy-specific salt-sensitive postpartum hypertension and vascular dysfunction via disruption of NO/cGMP/PKG pathway†.","authors":"Ruolin Song, Pankaj Yadav, Alissa Hofmann, Jay S Mishra, Sathish Kumar","doi":"10.1093/biolre/ioaf289","DOIUrl":"10.1093/biolre/ioaf289","url":null,"abstract":"Obstructive sleep apnea (OSA), common in pregnancy, is linked to preeclampsia and long-term maternal hypertension, but the underlying mechanisms remain unclear. We hypothesized that intermittent hypoxia (IH), a core feature of OSA, primes the maternal vasculature for heightened responses to hypertensive stressors postpartum. Pregnant Sprague-Dawley rats were exposed to either room air or gestational IH from gestational days 10-21. Two months after delivery, all dams were subjected to a high-salt challenge (2% NaCl in drinking water). Blood pressure and heart rate were measured during gestation and before and following salt loading. Vascular function and protein expression were analyzed in mesenteric arteries at study completion. A parallel group of nonpregnant rats underwent identical IH and salt exposure to delineate pregnancy-specific effects. IH exposure in pregnancy led to gestational hypertension that resolved postpartum, whereas nonpregnant rats showed no blood pressure changes. However, following high-salt challenge, postpartum IH-exposed dams displayed significantly greater increases in blood pressure and heart rate, abnormalities not seen in nonpregnant counterparts. Ex vivo, arteries from IH-exposed dams showed enhanced vasocontraction to phenylephrine and impaired vasodilation to both acetylcholine (endothelium-dependent) and sodium nitroprusside (endothelium-independent). Molecular analysis revealed increased endothelial nitric oxide synthase activation and decreased protein kinase G (PKG) in the IH group, with no change in α1-adrenergic receptor expression, indicating disrupted vascular smooth muscle signaling. Virgin rats showed no such alterations. Gestational IH induces a pregnancy-specific predisposition to postpartum salt-sensitive hypertension and vascular dysfunction, driven by disrupted PKG signaling. Early OSA detection and intervention in pregnancy may reduce cardiovascular risk.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1458-1468"},"PeriodicalIF":3.0,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145817537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The bull reproductive microbiome: a comparative analysis of microbial communities within semen and organs of the bull reproductive system†. 公牛生殖微生物组：公牛生殖系统精液和器官内微生物群落的比较分析

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-13 DOI: 10.1093/biolre/ioag025

Sarah A Retherford, Kelly L Woodruff, Bo R Harstine, Dana K Dittoe, Jeremy Block

{"title":"The bull reproductive microbiome: a comparative analysis of microbial communities within semen and organs of the bull reproductive system†.","authors":"Sarah A Retherford, Kelly L Woodruff, Bo R Harstine, Dana K Dittoe, Jeremy Block","doi":"10.1093/biolre/ioag025","DOIUrl":"10.1093/biolre/ioag025","url":null,"abstract":"Semen from mature, healthy bulls contains commensal microbes. Potential internal sources of seminal microbes have not been extensively evaluated. Objectives were to (1) assess whether the testes of the bull contain commensal microbes and to compare the composition of the putative testicular microbiome to that of semen and rumen fluid and (2) determine whether other organs of the bull reproductive system contain microbial populations. Here, we demonstrate that the testes of the bull contain a low biomass, yet diverse, microbiome. Interestingly, the microbial composition of rumen fluid, semen and testicular tissue were each dissimilar from one another, indicating that each source contains a unique microbiota. Only four core amplicon sequence variants, Acinetobacter, Enterobacteriaceae (Escherichia coli), Jeotgalicoccus, and Kiritimatiellae WCHB1-41, were shared between semen and the testes. Along with the testes, microbial populations were also present within the penile and pelvic urethra, seminal vesicles and epididymis of mature bulls. Of these anatomic niches, the microbial populations within the penile and pelvic urethra were the most diverse and shared the greatest number of core taxa (n = 72). The microbiota of the seminal vesicles, epididymis and the testes were significantly dissimilar from each other. Only one core taxa, Mycoplasma, was shared between the testes and epididymis. Collectively, our results demonstrate that semen and organs of the bull reproductive tract contain unique microbial populations. Further research is necessary to determine whether the microbial composition of organs of the bull reproductive system, such as the testes and epididymis, influence sperm viability and bull fertility.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1343-1358"},"PeriodicalIF":3.0,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146059832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of the Environmental Protection Agency reference dose of glyphosate on the ovarian function and cycle during the postnatal period. EPA标准剂量草甘膦对产后卵巢功能和周期的影响。

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-13 DOI: 10.1093/biolre/ioag037

Delfina Sol Ferman, Yamila Gamaleri, Marina Ercilia Dasso, Cecilia Lucía Centola, Mariana Cruz, Alejandro Lomniczi, Marina Cinthia Peluffo

引用次数: 0

Immune status in the mouse oviduct and uterine lumen across early embryogenesis to embryo implantation†. 小鼠输卵管和子宫腔在胚胎发生早期到胚胎着床期间的免疫状态

IF 3 2区生物学

Biology of Reproduction Pub Date : 2026-04-13 DOI: 10.1093/biolre/ioaf257

Liyou An, Meixiang Ma, Liang Zhang, Chenxu Li, Danni Fan, Yuzhu Mo, Linmei Duan, Wenrong Li, Xinrong Peng

{"title":"Immune status in the mouse oviduct and uterine lumen across early embryogenesis to embryo implantation†.","authors":"Liyou An, Meixiang Ma, Liang Zhang, Chenxu Li, Danni Fan, Yuzhu Mo, Linmei Duan, Wenrong Li, Xinrong Peng","doi":"10.1093/biolre/ioaf257","DOIUrl":"10.1093/biolre/ioaf257","url":null,"abstract":"In mammalian early pregnancy, maternal immune cells are critical during early pregnancy for initiating embryogenesis and maintaining implantation. However, the preimplantation immune environment within the reproductive tract is largely uncharacterized. Here, we detected a massive influx of immune cells into the lumen of the oviduct and uterus after mating in mice. In immune cells, CD11b+ cells up to about 38.1% in the oviduct and 59.5% in the uterus, of which neutrophils accounted for 81.7% and 97.7%, respectively. Neutrophils presented an antigen-presenting cells like phenotype (nAPCs), a specialized population expressing Ly6G and MHCII. We followed the dynamic changes and fate of neutrophils, and found that these cells rapidly disappeared from the lumen of the reproductive tract from 0.5 days post-coitus (dpc) to 1.5 dpc. Instead of apoptosis, they migrated from the oviduct to the uterus and infiltrated the endometrium at 1.0-1.5 dpc. At 3.5 dpc, the number of immune cells decreased and natural killer (NK) cells became the predominant population. The cytokines involved in modulating immune cell responses (GM-CSF, IFN-γ and IL17A), pro-inflammation (IL-1β, IL-6, IL-12p70, TNF-α), and anti-inflammation (IL-10) were detected in luminal fluid, in which GM-CSF and IL-6 decreased, IL-10 increased in 3.5 dpc uterus. However, spontaneous abortion mice (CBA/J × DBA/2) exhibited altered immune patterns at the stage of embryogenesis and implantation. Our findings reveal a dynamic and finely tuned immune state in the lumen of the oviduct and uterus, which provide a microenvironment for early embryo development, and provide insights into pre-implantation embryo-uterine cross-talk in mice.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":" ","pages":"1277-1294"},"PeriodicalIF":3.0,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145562528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0