Biology of Reproduction最新文献_第4页

Delayed endometrial preparation for the induction of luteolysis as a potential factor for improved reproductive performance in Angus beef heifers with high antral follicle counts. 延迟子宫内膜准备以诱导黄体溶解是提高高前卵泡数安格斯肉用小母牛繁殖性能的潜在因素。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-10-10 DOI: 10.1093/biolre/ioae146

Martim Kaps, Lacey K Quail, Shelby L Rosasco, Alexandria P Snider, Saulo M Zoca, Kaitlin M Epperson, Jerica J J Rich, Jeremy R Miles, Matthew S Crouse, Brittney N Keel, Adam F Summers, George A Perry, Clay A Lents, Robert A Cushman

{"title":"Delayed endometrial preparation for the induction of luteolysis as a potential factor for improved reproductive performance in Angus beef heifers with high antral follicle counts.","authors":"Martim Kaps, Lacey K Quail, Shelby L Rosasco, Alexandria P Snider, Saulo M Zoca, Kaitlin M Epperson, Jerica J J Rich, Jeremy R Miles, Matthew S Crouse, Brittney N Keel, Adam F Summers, George A Perry, Clay A Lents, Robert A Cushman","doi":"10.1093/biolre/ioae146","DOIUrl":"https://doi.org/10.1093/biolre/ioae146","url":null,"abstract":"Antral Follicle Count (AFC) and anti-Müllerian hormone (AMH) concentrations are reflective for ovarian reserve and have been associated with improved reproductive performance in cattle. Key events for regulation of uterine receptivity are orchestrated by progesterone. As progesterone concentrations are greater in animals with high than low AFC, we tested the hypothesis, if the resulting improved uterine environment will lead to improved conceptus elongation and endometrial response to interferon tau. For four years, 10 heifers with lowest and highest AFC, respectively, were selected from 120 heifers. Reproductive tracts and blood samples for progesterone and AMH analysis were collected after synchronization and insemination. For a recovered conceptus, length was determined, and interferon tau (IFNT) transcript abundance was analyzed. Endometrial transcript abundance of interferon-stimulated gene 15 (ISG15) and oxytocin receptor (OXTR) were analyzed. Progesterone concentrations did not differ between Low and High AFC Group (P = 0.1). A difference in conceptus length was not observed. Endometrial abundance of ISG15 did not differ between Pregnant Low and High AFC heifers. Abundance of OXTR was greater in Open Low AFC than Open High AFC heifers (P < 0.01). Interaction of AMH and Pregnancy Status was determined, with greater AMH in Pregnant than Open High AFC heifers (P < 0.05). Improved uterine environment in High vs. Low AFC heifers did not result in longer conceptuses or improved endometrial response. As the increase in OXTR transcript abundance was only detected in Low AFC heifers, reported differences in reproductive performance might be associated with earlier initiation of luteolysis.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Memorium of Professor Patricia B. Hoyer. 纪念帕特里夏-B-霍耶教授。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-10-10 DOI: 10.1093/biolre/ioae145

Jodi A Flaws, Zelieann Craig, Aileen Keating

引用次数: 0

Correction to: Single-cell RNA sequencing reveals the important role of Dcaf17 in spermatogenesis of golden hamsters. 更正为单细胞 RNA 测序揭示 Dcaf17 在金色仓鼠精子发生过程中的重要作用

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-10-08 DOI: 10.1093/biolre/ioae144

引用次数: 0

Development of KISS1 knockout pigs is characterized by hypogonadotropic hypogonadism, normal growth, and reduced skatole. KISS1 基因敲除猪的发育特点是性腺功能减退、生长正常和皮脂减少。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-10-07 DOI: 10.1093/biolre/ioae140

Daniel F Ahern, Kyra Martins, Julio M Florez, Caitlin E Ross, Abe Huisman, Robert A Cushman, Sydney L Shuping, Casey C Nestor, Amy T Desaulniers, Brett R White, Tad S Sonstegard, Clay A Lents

{"title":"Development of KISS1 knockout pigs is characterized by hypogonadotropic hypogonadism, normal growth, and reduced skatole.","authors":"Daniel F Ahern, Kyra Martins, Julio M Florez, Caitlin E Ross, Abe Huisman, Robert A Cushman, Sydney L Shuping, Casey C Nestor, Amy T Desaulniers, Brett R White, Tad S Sonstegard, Clay A Lents","doi":"10.1093/biolre/ioae140","DOIUrl":"https://doi.org/10.1093/biolre/ioae140","url":null,"abstract":"Kisspeptin is a major regulator of gonadotropin secretion in pigs. Previously, CRISPR/Cas9 knockout of KISS1 was used to develop a mosaic parental line of pigs to generate offspring that would not need castration due to loss of kisspeptin. The current goal was to characterize growth and reproductive development of F1 pigs from this parental line. Body weights, gonadotropin concentrations and gonadal development were measured from birth through development (boars to 220 d of age, n = 42; gilts to 160 d of age, n = 36). Testosterone, skatole, and androstenone were also measured in boars. Blood samples were collected by jugular venipuncture for quantification of serum hormones, gonadal tissues collected for gross morphology and histology, and a fat biopsy collected (boars) for skatole and androstenone analysis. Body weight did not differ with genotype. There were no differences between KISS1+/+ and heterozygote KISS1+/- animals for most parameters measured. Gonadotropin concentrations were reduced in KISS1-/- boars and gilts compared with KISS1+/+ and KISS1+/- animals (P < 0.05). Concentrations of testosterone in serum and both androstenone and skatole in adipose were less in KISS1-/- boars than in KISS1+/+ and KISS1+/- boars (P < 0.05). Hypogonadism was in all KISS1-/- gilts and boars. These data indicate that knocking out KISS1 causes hypogonadotropic hypogonadism but does not negatively affect growth in pigs. Only one KISS1 allele is needed for normal gonadotropin secretion and gonadal development, and accumulation of compounds in adipose leading to boar taint.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fetal hypoxia exposure induced Hif1a activation and autophagy in adult ovary granulosa cells. 胎儿缺氧诱导成年卵巢颗粒细胞中的Hif1a活化和自噬。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-10-03 DOI: 10.1093/biolre/ioae141

Lu-Yao Zhang, Ke Zhang, Xi Zhao, Hai-Ping Tao, Gong-Xue Jia, You-Gui Fang, Yun-Peng Hou, Qi-En Yang

{"title":"Fetal hypoxia exposure induced Hif1a activation and autophagy in adult ovary granulosa cells.","authors":"Lu-Yao Zhang, Ke Zhang, Xi Zhao, Hai-Ping Tao, Gong-Xue Jia, You-Gui Fang, Yun-Peng Hou, Qi-En Yang","doi":"10.1093/biolre/ioae141","DOIUrl":"https://doi.org/10.1093/biolre/ioae141","url":null,"abstract":"Environmental hypoxia adversely impacts the reproduction of humans and animals. Previously, we showed that fetal hypoxia exposure led to granulosa cell (GC) autophagic cell death via the Foxo1/Pi3k/Akt pathway. However, the upstream regulatory mechanisms underlying GC dysfunction remain largely unexplored. Here, we tested the hypothesis that fetal hypoxia exposure altered gene expression programs in adult GCs and impaired ovarian function. We established a fetal hypoxia model in which pregnant mice were maintained in a high-plateau hypoxic environment from gestation day (E) 0--16.5 to study the impact of hypoxia exposure on the ovarian development and subsequent fertility of offspring. Compared with the unexposed control, fetal hypoxia impaired fertility by disordering ovarian function. Specifically, fetal hypoxia caused mitochondrial dysfunction, oxidant stress and autophagy in GCs in the adult ovary. RNA-seq analysis revealed that 437 genes were differentially expressed in the adult GCs of exposed animals. Western blotting results also revealed that fetal exposure induced high levels of hypoxia-inducible factor 1-alpha (Hif1a) expression in adult GCs. We then treated GCs isolated from exposed mice with PX-478, a specific pharmacological inhibitor of Hif-1a, and found that autophagy and apoptosis were effectively alleviated. Finally, by using a human ovarian granulosa-like tumor cell line (KGN) to simulate hypoxia in vitro, we showed that Hif1a regulated autophagic cell death in GCs through the Pi3k/Akt pathway. Together, these findings suggest that fetal hypoxia exposure induced persistent Hif1a expression, which impaired mitochondrial function and led to autophagic cell death in the GCs of the adult ovary.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypoxia-inducible factors (HIFs) in early pregnancy: implications for miscarriage†. 妊娠早期的低氧诱导因子（HIFs）：对流产的影响†。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-26 DOI: 10.1093/biolre/ioae139

Yuxuan Lai, Zhiyu Fu, Yaxin Gao, Ning Ma, Lu Li

{"title":"Hypoxia-inducible factors (HIFs) in early pregnancy: implications for miscarriage†.","authors":"Yuxuan Lai, Zhiyu Fu, Yaxin Gao, Ning Ma, Lu Li","doi":"10.1093/biolre/ioae139","DOIUrl":"https://doi.org/10.1093/biolre/ioae139","url":null,"abstract":"Miscarriage poses a significant threat to both maternal and fetal health. Its etiology remains unknown, and there are no established effective identification or prevention strategies. A low oxygen environment in early pregnancy is a physiological necessity for embryonic and placental growth. Hypoxia-inducible factors (HIFs) are a family of classic hypoxia signaling molecules, whose expression level may fluctuate abnormally because of imbalance in oxygen levels. Its unusual fluctuations initiate multiple signaling pathways at the maternal-fetal interface and modulate a range of phenotypic changes, including proliferation, differentiation, migration, invasion, angiogenesis, endometrial decidualization, and immune tolerance. Notably, aberrant regulation of these processes may lead to miscarriage. This review aims to clarify how HIF-1α mediates the aberrant regulation of biological processes, including autophagy, metabolic reprogramming, immunity, epigenetics, and angiogenesis, and how these effects impact trophoblasts and other cells at the maternal-fetal interface. These findings provide new insights into potential therapeutic and preventive strategies for miscarriage.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The RNA-binding protein road map of spermatogenesis. 精子发生的 RNA 结合蛋白路线图

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-24 DOI: 10.1093/biolre/ioae138

P Jeremy Wang

引用次数: 0

Replacing sperm with genotyped haploid androgenetic blastomeres to generate cattle with predetermined paternal genomes. 用基因分型的单倍体雄性胚泡替代精子，生成具有预定父系基因组的牛。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-20 DOI: 10.1093/biolre/ioae135

Luis Aguila, Rafael V Sampaio, Jacinthe Therrien, Ricardo P Nociti, Remi Labrecque, Amelie Tremblay, Gabrielle Marras, Patrick Blondin, Lawrence C Smith

{"title":"Replacing sperm with genotyped haploid androgenetic blastomeres to generate cattle with predetermined paternal genomes.","authors":"Luis Aguila, Rafael V Sampaio, Jacinthe Therrien, Ricardo P Nociti, Remi Labrecque, Amelie Tremblay, Gabrielle Marras, Patrick Blondin, Lawrence C Smith","doi":"10.1093/biolre/ioae135","DOIUrl":"https://doi.org/10.1093/biolre/ioae135","url":null,"abstract":"Although meiosis plays an essential role for the survival of species in natural selection, the genetic diversity resulting from sexual reproduction impedes human-driven strategies to transmit the most suitable genomes for genetic improvement, forcing breeders to select diploid genomes generated after fertilization, that is, after the encounter of sperm and oocytes carrying unknown genomes. To determine whether genomic assessment could be used before fertilization, some androgenetic haploid morula-stage bovine embryos derived from individual sperm were biopsied for genomic evaluation and others used to reconstruct \"semi-cloned\" (SC) diploid zygotes by the intracytoplasmic injection into parthenogenetically activated oocytes, and the resulting embryos were transferred to surrogate females to obtain gestations. Compared to controls, in vitro development to the blastocyst stage was lower and fewer surrogates became pregnant from the transfer of SC embryos. However, fetometric measurements of organs and placental membranes of all SC conceptuses were similar to controls, suggesting a normal post-implantation development. Moreover, transcript amounts of imprinted genes IGF2, IGF2R, PHLDA2, SNRPN and KCNQ1OT1 and methylation pattern of the KCNQ1 DMR were unaltered in SC conceptuses. Overall, this study shows that sperm can be replaced by genotyped haploid embryonic-derived cells to produce bovine embryos carrying a predetermined paternal genome and viable first trimester fetuses after transfer to female recipients.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142280109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The loss of dNK1/2 and EVT1 cells at the maternal-fetal Interface is associated with recurrent miscarriage. 母胎界面 dNK1/2 和 EVT1 细胞的丢失与反复流产有关。

IF 3.1 2区生物学

Biology of Reproduction Pub Date : 2024-09-20 DOI: 10.1093/biolre/ioae136

Yijun Yang, Jiangnan Qiu, Qiaoqiao Xu, Yun Fan, Hui Wang, Hong Qian, Zhu Wu, Yuchen Zhang, Yingchun Gao, Can Shi, Chuncheng Lu, Yankai Xia, Wenjun Cheng

{"title":"The loss of dNK1/2 and EVT1 cells at the maternal-fetal Interface is associated with recurrent miscarriage.","authors":"Yijun Yang, Jiangnan Qiu, Qiaoqiao Xu, Yun Fan, Hui Wang, Hong Qian, Zhu Wu, Yuchen Zhang, Yingchun Gao, Can Shi, Chuncheng Lu, Yankai Xia, Wenjun Cheng","doi":"10.1093/biolre/ioae136","DOIUrl":"https://doi.org/10.1093/biolre/ioae136","url":null,"abstract":"Recurrent miscarriage (RM) is a chronic and heterogeneous pregnancy disorder lacking effective treatment. Alterations at the maternal-fetal interface are commonly observed in RM, with the loss of certain cell subpopulations believed to be a key cause. Through single-cell sequencing of RM patients and healthy donors, we aim to identify aberrancy of cellular features in RM tissues, providing new insights into the research. Natural killer (NK) cells, the most abundant immune cells in the decidua, are traditionally classified into dNK1, dNK2, and dNK3. In this study, we identified a new subset, dNK1/2, absent in RM tissues. This subset was named because it expresses biomarkers of both dNK1 and dNK2. With further analysis, we discovered that dNK1/2 cells play roles in immunoregulation and cytokine secretion. On the villous side of the interface, a notable decrease of extravillous trophoblast (EVT) cells was identified in RM tissues. We clustered EVTs into EVT1 (absent in RM) and EVT2 (retained in RM). Pseudotime analysis revealed distinct differentiation paths, identifying CCNB1, HMGB1, and NPM1 as EVT1 biomarkers. Additionally, we found that EVT1 is involved in the regulation of cell death, while EVT2 exhibited more angiogenic activity. Cell communication analysis revealed that interaction between EVT1 and dNK1/2 mediates chemotaxis and endothelial cell regulation, crucial for spiral artery remodeling. The loss of this interaction may impair decidualization, which is associated with RM. In summary, we propose that the loss of dNK1/2 and EVT1 cells is a significant pathological feature of RM.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142280110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of CCDC188 causes male infertility with defects in the sperm head–neck connection in mice CCDC188 缺失会导致小鼠精子头颈部连接缺陷，从而导致雄性不育

IF 3.6 2区生物学

Biology of Reproduction Pub Date : 2024-09-18 DOI: 10.1093/biolre/ioae137

Yumiao Qiu, Keisuke Shimada, Kaito Yamamoto, Masahito Ikawa

{"title":"Loss of CCDC188 causes male infertility with defects in the sperm head–neck connection in mice","authors":"Yumiao Qiu, Keisuke Shimada, Kaito Yamamoto, Masahito Ikawa","doi":"10.1093/biolre/ioae137","DOIUrl":"https://doi.org/10.1093/biolre/ioae137","url":null,"abstract":"Acephalic spermatozoa syndrome (ASS) represents a rare genetic and reproductive disease, which is defined as semen composed of mostly headless spermatozoa. The connecting piece in the neck region, also known as the head-to-tail coupling apparatus (HTCA), plays a crucial role in the tight linkage between the sperm head and tail. Dysfunction of this structure can lead to separation of sperm heads and tails, and male infertility. Using the mouse as an experimental model, several proteins have been identified as associated with the HTCA and disruption of these proteins causes acephalic spermatozoa. However, the molecular mechanism underlying this morphologic anomaly and HTCA remains elusive. In this study, we focused on coiled-coil domain containing 188 (Ccdc188), which shows testis-enriched expression. To elucidate the physiological role of CCDC188, we generated a knockout (KO) mouse line using the CRISPR/Cas9 system. Ccdc188 KO male mice were sterile, indicating that CCDC188 is indispensable for male fertility. Most Ccdc188-null spermatozoa were acephalic. Transmission electron microscopy revealed that while the sperm HTCA could assemble properly without CCDC188, the HTCA failed to attach to the nucleus during spermiogenesis, leading to sperm head and neck separation. In addition, we found almost all of the spermatozoa in the cauda epididymis lacked a mitochondrial sheath. Taken together, we demonstrated that CCDC188 plays a crucial role in forming a tight sperm head–neck junction.","PeriodicalId":8965,"journal":{"name":"Biology of Reproduction","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0