Biotechnology and Bioprocess Engineering最新文献_第6页

Improved enzyme-linked immunosorbent assay using surface-adhesive antibody-oriented immobilizing biolinker: a proof-of-concept study 使用面向抗体的表面粘附固定生物链接剂改进酶联免疫吸附测定法：概念验证研究

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-27 DOI: 10.1007/s12257-024-00093-7

Ae Sol Lee, Hye Ryoung Heo, Chang Sup Kim, Hyung Joon Cha

引用次数: 0

Oligopeptides derived from rice protein hydrolysates: absorption, bioactivity, preparation, debittering, and application 从大米蛋白水解物中提取的寡肽：吸收、生物活性、制备、去杂质和应用

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-25 DOI: 10.1007/s12257-024-00092-8

Zheng-Fei Yan, Yi-Mei Cai, Yong-Kun Xiao, Shuai Yuan, Xue-Yi Qiao, Bo Xu, Jing Wu

引用次数: 0

3D microcapsules for human bone marrow-derived mesenchymal stem cell biomanufacturing in a vertical-wheel bioreactor 用于在垂直轮式生物反应器中进行人类骨髓间充质干细胞生物制造的三维微囊

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-21 DOI: 10.1007/s12257-024-00069-7

引用次数: 0

Development of a glucose enzyme fuel cell based on thin film electrode using biocatalysts 利用生物催化剂开发基于薄膜电极的葡萄糖酶燃料电池

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-21 DOI: 10.1007/s12257-024-00065-x

Dong Sup Kim, Xiaoguang Yang, Abdus Sobhan, Chulhwan Park, Seung Wook Kim, Jinyoung Lee

{"title":"Development of a glucose enzyme fuel cell based on thin film electrode using biocatalysts","authors":"Dong Sup Kim, Xiaoguang Yang, Abdus Sobhan, Chulhwan Park, Seung Wook Kim, Jinyoung Lee","doi":"10.1007/s12257-024-00065-x","DOIUrl":"https://doi.org/10.1007/s12257-024-00065-x","url":null,"abstract":"Artificial transplantation of the human body, which requires high technology, has been an attractive issue in the 4th industrial revolution era. The artificial equipment for human applications could contain a small-scale power supply. Enzyme fuel cells (EFCs) that generate green energy are being researched for use as the power supply for pacemakers, insulin pump, and retinal implant in human body. This study focused on an (EFC) using thin film electrodes-based on enzyme immobilization technology. The performance of this EFC was improved by enzyme immobilization and electron transfer. To improve the electron transfer, the GO/Co/chitosan composite was modified on the surface of thin film electrode. The properties of this modified surface of thin film electrode were confirmed by analysis of field emission gun scanning electron microscopy, Fourier transform infrared spectroscopy, and atomic force microscopy. The performance of the designed EFC was optimized with immobilized redox enzyme on the modified electrode. The highest power density and voltage are determined as 441.48 µW/cm2 and − 0.443 V by thin film electrode, respectively. The optimum conditions of the EFC were 0.1 M D-glucose, 0.1 g/L glucose oxidase, pH 7.0, and reaction time of 4 h for both two types of thin film-electrodes.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"165 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140198069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-drug-loaded MSCs-derived exosomal vesicles inhibit endometrial cancer cell proliferation by promoting apoptosis through the migration and invasion of Rac1/NF-κB/MMP2 signalling pathway 通过Rac1/NF-κB/MMP2信号通路的迁移和侵袭促进凋亡，从而抑制子宫内膜癌细胞增殖

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-18 DOI: 10.1007/s12257-024-00088-4

{"title":"Dual-drug-loaded MSCs-derived exosomal vesicles inhibit endometrial cancer cell proliferation by promoting apoptosis through the migration and invasion of Rac1/NF-κB/MMP2 signalling pathway","authors":"","doi":"10.1007/s12257-024-00088-4","DOIUrl":"https://doi.org/10.1007/s12257-024-00088-4","url":null,"abstract":" <h3>Abstract</h3> Endometrial carcinoma affects the uterine lining. Endogenous activity, intrinsic targeting, and ability to engage with a host defence system make exosomal vehicles (EVs) a viable cancer treatment alternative. Due to these benefits, mesenchymal stem cell (MSC)-derived EVs loaded with carboplatin and paclitaxel could resemble immune cells to fight cancer. This study found that Car-Pac@EVs downregulated endometrial cancer (EC) cells relative to normal endometrium. Car-Pac@EVs' effects on ECC-1 and HEC-1A EC cells at different doses were examined in vitro. To detect cancer, MTT, flow cytometry, and transwell assays were used. Protein expression was measured by Western blotting and qRT-PCR. Car-Pac@EVs were affected by time- and dose-dependent EC cell proliferation reductions. In EC cells, Car-Pac@EVs triggered apoptosis. Car-Pac@EVs formulation reduced EC cell migration and invasion by reducing MMP-2 expression via Rac1/NF-κB signalling. The results indicated that Car-Pac@EVs may be an effective EC diagnosis and treatment target. <h3>Graphical abstract</h3> <img alt=\"\" src=\"https://static-content.springer.com/image/MediaObjects/12257_2024_88_Figa_HTML.png\"/> ","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"56 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140166706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-free systems for a multi-pronged approach to next-generation therapeutics and diagnostics 多管齐下的新一代治疗和诊断无细胞系统

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-17 DOI: 10.1007/s12257-024-00058-w

引用次数: 0

Development and evaluation of orbital rocking motion-based single-use cell culture system, the CELBIC® system 开发和评估基于轨道摇摆运动的一次性使用细胞培养系统 CELBIC® 系统

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-16 DOI: 10.1007/s12257-024-00090-w

Hyunwoo Kim, Seohyun Park, Rock Ki Kim, Yeong Ok Baik, Kyung Nam Kim, Duk Jae Oh

引用次数: 0

Establishment of an optimized electroporation method for Halomonas sp. YK44 and its application in the coproduction of PHB and isobutanol 建立哈洛单胞菌 YK44 的优化电穿孔方法及其在 PHB 和异丁醇共生中的应用

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-15 DOI: 10.1007/s12257-024-00055-z

{"title":"Establishment of an optimized electroporation method for Halomonas sp. YK44 and its application in the coproduction of PHB and isobutanol","authors":"","doi":"10.1007/s12257-024-00055-z","DOIUrl":"https://doi.org/10.1007/s12257-024-00055-z","url":null,"abstract":"<h3>Abstract</h3> Many Halomonas spp. thrive in high-salinity environments, and their resistance to high salt levels allows for their cultivation in non-sterile conditions. Despite their robustness and potential poly (3-hydroxybutyrate) (PHB) production capability, there are relatively few reports on the engineering of various Halomonas species, and there are still some difficulties in genetically engineering novel Halomonas strains. In particular, conjugation as a transformation method has been employed more frequently than electroporation in Halomonas; however, electroporation is necessary for the accelerated engineering of Halomonas and increased time efficiency. To touch this issue, we collected Halomonas strains and evaluated their PHB production and electroporation efficiencies resulting that the Halomonas sp. YK44 showed the highest electroporation efficiency with high PHB production among the various Halomonas strains. A series of electroporation protocol optimization experiments were conducted to identify optimal conditions for Halomonas sp. YK44 such as main culturing for 10 h, utilizing a DNA concentration of 150–200 μg/mL, and performing electroporation at 2.1 kV, followed by a washing step using 10% glycerol and a recovery period of 36 h with pBBR1MCS2. By introducing isobutanol biosynthetic genes using an optimized electroporation protocol, the highest isobutanol production was obtained at 196 mg/L with 63% PHB content simultaneously and the higher PHB production was obtained at 6.6 g/L with 152 mg/L isobutanol. Our approach showed the overall process to identify a suitable Halomonas host by applying general electroporation methods, optimizing electroporation protocols, and demonstrated the first coproduction of PHB and isobutanol in Halomonas.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"1 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140148043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photothermal heating of cell-free reactions for on-site production of recombinant proteins 现场生产重组蛋白质的无细胞反应光热加热技术

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-14 DOI: 10.1007/s12257-024-00051-3

Kyunghwan Yeom, Yu Jin Park, Hansol Kim, Dong-Yeon Song, Dong-Myung Kim, Ji-Ho Park

{"title":"Photothermal heating of cell-free reactions for on-site production of recombinant proteins","authors":"Kyunghwan Yeom, Yu Jin Park, Hansol Kim, Dong-Yeon Song, Dong-Myung Kim, Ji-Ho Park","doi":"10.1007/s12257-024-00051-3","DOIUrl":"https://doi.org/10.1007/s12257-024-00051-3","url":null,"abstract":"Cell-free synthesis technology is emerging as a versatile platform for biomanufacturing, particularly for on-site production of essential products. In this study, we address a pivotal challenge encountered when utilizing cell-free protein synthesis (CFPS) for on-site production in resource-limited settings. The efficacy of CFPS critically depends on precise temperature regulation. However, traditional heating methods are impractical for application outside of laboratory environments. To address this challenge, we propose an innovative solution that integrates gold nanorods (GNRs) with photothermal properties into the CFPS reaction mixture. Upon activation by a handheld laser module emitting near-infrared light, these GNRs efficiently convert light energy into heat, enabling rapid and precise temperature control for protein synthesis. Our approach not only achieves optimal synthesis temperatures more rapidly than conventional methods but also significantly enhances protein yields, with an increase of over threefold within a 30-min reaction period. In summary, our findings highlight the potential of photothermal heating as a means to ensure portable and efficient protein synthesis in the field. This advancement brings CFPS closer to achieving real-time, on-site protein production.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"130 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140128763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of antioxidants to extend the storage of lyophilized cell-free synthesis system 使用抗氧化剂延长冻干无细胞合成系统的储存时间

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-13 DOI: 10.1007/s12257-024-00054-0

Kyu Jae Kim, So Jeong Lee, Dong-Myung Kim

{"title":"Use of antioxidants to extend the storage of lyophilized cell-free synthesis system","authors":"Kyu Jae Kim, So Jeong Lee, Dong-Myung Kim","doi":"10.1007/s12257-024-00054-0","DOIUrl":"https://doi.org/10.1007/s12257-024-00054-0","url":null,"abstract":"Modern chemical processes, vital for diverse product manufacturing, often result in substantial energy consumption and environmental waste. As environmental concerns continue to escalate and industries evolve to meet customized demands, biomanufacturing emerges as a promising alternative due to its efficiency, expandability, and eco-friendliness. Cell-free synthesis systems, which harness cellular extracts for biosynthetic reactions, offer a highly adaptable solution for biomanufacturing, particularly when rapid production is required with limited resources. While conventional cell-free systems encounter challenges related to storage and transportation due to the necessity for ultra-cold temperatures, recent studies have demonstrated that these systems can be lyophilized and rehydrated to enable on-demand biomolecule production. Our study aims to enhance the stability of lyophilized cell-free systems. We have discovered the significant role played by antioxidants, specifically dithiothreitol and sodium nitrite, in preserving translational activity during extended storage. This finding represents a significant step forward for decentralized, on-demand protein production using cell-free methods.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"25 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140128668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0