Biotechnology and Bioprocess Engineering最新文献_第6页

Dual-drug-loaded MSCs-derived exosomal vesicles inhibit endometrial cancer cell proliferation by promoting apoptosis through the migration and invasion of Rac1/NF-κB/MMP2 signalling pathway 通过Rac1/NF-κB/MMP2信号通路的迁移和侵袭促进凋亡，从而抑制子宫内膜癌细胞增殖

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-18 DOI: 10.1007/s12257-024-00088-4

{"title":"Dual-drug-loaded MSCs-derived exosomal vesicles inhibit endometrial cancer cell proliferation by promoting apoptosis through the migration and invasion of Rac1/NF-κB/MMP2 signalling pathway","authors":"","doi":"10.1007/s12257-024-00088-4","DOIUrl":"https://doi.org/10.1007/s12257-024-00088-4","url":null,"abstract":" <h3>Abstract</h3> Endometrial carcinoma affects the uterine lining. Endogenous activity, intrinsic targeting, and ability to engage with a host defence system make exosomal vehicles (EVs) a viable cancer treatment alternative. Due to these benefits, mesenchymal stem cell (MSC)-derived EVs loaded with carboplatin and paclitaxel could resemble immune cells to fight cancer. This study found that Car-Pac@EVs downregulated endometrial cancer (EC) cells relative to normal endometrium. Car-Pac@EVs' effects on ECC-1 and HEC-1A EC cells at different doses were examined in vitro. To detect cancer, MTT, flow cytometry, and transwell assays were used. Protein expression was measured by Western blotting and qRT-PCR. Car-Pac@EVs were affected by time- and dose-dependent EC cell proliferation reductions. In EC cells, Car-Pac@EVs triggered apoptosis. Car-Pac@EVs formulation reduced EC cell migration and invasion by reducing MMP-2 expression via Rac1/NF-κB signalling. The results indicated that Car-Pac@EVs may be an effective EC diagnosis and treatment target. <h3>Graphical abstract</h3> <img alt=\"\" src=\"https://static-content.springer.com/image/MediaObjects/12257_2024_88_Figa_HTML.png\"/> ","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"56 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140166706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-free systems for a multi-pronged approach to next-generation therapeutics and diagnostics 多管齐下的新一代治疗和诊断无细胞系统

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-17 DOI: 10.1007/s12257-024-00058-w

引用次数: 0

Development and evaluation of orbital rocking motion-based single-use cell culture system, the CELBIC® system 开发和评估基于轨道摇摆运动的一次性使用细胞培养系统 CELBIC® 系统

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-16 DOI: 10.1007/s12257-024-00090-w

Hyunwoo Kim, Seohyun Park, Rock Ki Kim, Yeong Ok Baik, Kyung Nam Kim, Duk Jae Oh

引用次数: 0

Establishment of an optimized electroporation method for Halomonas sp. YK44 and its application in the coproduction of PHB and isobutanol 建立哈洛单胞菌 YK44 的优化电穿孔方法及其在 PHB 和异丁醇共生中的应用

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-15 DOI: 10.1007/s12257-024-00055-z

{"title":"Establishment of an optimized electroporation method for Halomonas sp. YK44 and its application in the coproduction of PHB and isobutanol","authors":"","doi":"10.1007/s12257-024-00055-z","DOIUrl":"https://doi.org/10.1007/s12257-024-00055-z","url":null,"abstract":"<h3>Abstract</h3> Many Halomonas spp. thrive in high-salinity environments, and their resistance to high salt levels allows for their cultivation in non-sterile conditions. Despite their robustness and potential poly (3-hydroxybutyrate) (PHB) production capability, there are relatively few reports on the engineering of various Halomonas species, and there are still some difficulties in genetically engineering novel Halomonas strains. In particular, conjugation as a transformation method has been employed more frequently than electroporation in Halomonas; however, electroporation is necessary for the accelerated engineering of Halomonas and increased time efficiency. To touch this issue, we collected Halomonas strains and evaluated their PHB production and electroporation efficiencies resulting that the Halomonas sp. YK44 showed the highest electroporation efficiency with high PHB production among the various Halomonas strains. A series of electroporation protocol optimization experiments were conducted to identify optimal conditions for Halomonas sp. YK44 such as main culturing for 10 h, utilizing a DNA concentration of 150–200 μg/mL, and performing electroporation at 2.1 kV, followed by a washing step using 10% glycerol and a recovery period of 36 h with pBBR1MCS2. By introducing isobutanol biosynthetic genes using an optimized electroporation protocol, the highest isobutanol production was obtained at 196 mg/L with 63% PHB content simultaneously and the higher PHB production was obtained at 6.6 g/L with 152 mg/L isobutanol. Our approach showed the overall process to identify a suitable Halomonas host by applying general electroporation methods, optimizing electroporation protocols, and demonstrated the first coproduction of PHB and isobutanol in Halomonas.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"1 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140148043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photothermal heating of cell-free reactions for on-site production of recombinant proteins 现场生产重组蛋白质的无细胞反应光热加热技术

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-14 DOI: 10.1007/s12257-024-00051-3

Kyunghwan Yeom, Yu Jin Park, Hansol Kim, Dong-Yeon Song, Dong-Myung Kim, Ji-Ho Park

{"title":"Photothermal heating of cell-free reactions for on-site production of recombinant proteins","authors":"Kyunghwan Yeom, Yu Jin Park, Hansol Kim, Dong-Yeon Song, Dong-Myung Kim, Ji-Ho Park","doi":"10.1007/s12257-024-00051-3","DOIUrl":"https://doi.org/10.1007/s12257-024-00051-3","url":null,"abstract":"Cell-free synthesis technology is emerging as a versatile platform for biomanufacturing, particularly for on-site production of essential products. In this study, we address a pivotal challenge encountered when utilizing cell-free protein synthesis (CFPS) for on-site production in resource-limited settings. The efficacy of CFPS critically depends on precise temperature regulation. However, traditional heating methods are impractical for application outside of laboratory environments. To address this challenge, we propose an innovative solution that integrates gold nanorods (GNRs) with photothermal properties into the CFPS reaction mixture. Upon activation by a handheld laser module emitting near-infrared light, these GNRs efficiently convert light energy into heat, enabling rapid and precise temperature control for protein synthesis. Our approach not only achieves optimal synthesis temperatures more rapidly than conventional methods but also significantly enhances protein yields, with an increase of over threefold within a 30-min reaction period. In summary, our findings highlight the potential of photothermal heating as a means to ensure portable and efficient protein synthesis in the field. This advancement brings CFPS closer to achieving real-time, on-site protein production.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"130 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140128763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of antioxidants to extend the storage of lyophilized cell-free synthesis system 使用抗氧化剂延长冻干无细胞合成系统的储存时间

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-13 DOI: 10.1007/s12257-024-00054-0

Kyu Jae Kim, So Jeong Lee, Dong-Myung Kim

{"title":"Use of antioxidants to extend the storage of lyophilized cell-free synthesis system","authors":"Kyu Jae Kim, So Jeong Lee, Dong-Myung Kim","doi":"10.1007/s12257-024-00054-0","DOIUrl":"https://doi.org/10.1007/s12257-024-00054-0","url":null,"abstract":"Modern chemical processes, vital for diverse product manufacturing, often result in substantial energy consumption and environmental waste. As environmental concerns continue to escalate and industries evolve to meet customized demands, biomanufacturing emerges as a promising alternative due to its efficiency, expandability, and eco-friendliness. Cell-free synthesis systems, which harness cellular extracts for biosynthetic reactions, offer a highly adaptable solution for biomanufacturing, particularly when rapid production is required with limited resources. While conventional cell-free systems encounter challenges related to storage and transportation due to the necessity for ultra-cold temperatures, recent studies have demonstrated that these systems can be lyophilized and rehydrated to enable on-demand biomolecule production. Our study aims to enhance the stability of lyophilized cell-free systems. We have discovered the significant role played by antioxidants, specifically dithiothreitol and sodium nitrite, in preserving translational activity during extended storage. This finding represents a significant step forward for decentralized, on-demand protein production using cell-free methods.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"25 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140128668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico screening and validation of different dehydrogenases to produce 2,3-butanediol in Bacillus subtilis 在枯草芽孢杆菌中生产 2,3-丁二醇的不同脱氢酶的硅学筛选和验证

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-13 DOI: 10.1007/s12257-024-00053-1

Sailee Sanjay Asolkar, M. Anju, Ravindra Kumar, Apoorva Deshmukh, Anand Ghosalkar, Pramod Kumbhar

{"title":"In silico screening and validation of different dehydrogenases to produce 2,3-butanediol in Bacillus subtilis","authors":"Sailee Sanjay Asolkar, M. Anju, Ravindra Kumar, Apoorva Deshmukh, Anand Ghosalkar, Pramod Kumbhar","doi":"10.1007/s12257-024-00053-1","DOIUrl":"https://doi.org/10.1007/s12257-024-00053-1","url":null,"abstract":"Bacillus subtilis is a natural producer of 2,3-butanediol (2,3-BDO) and has acquired “Generally Regarded as Safe\" status. It is reported to produce 2,3-BDO from synthetic sugars as well as complex and economic sugar sources such as molasses. However, the rate-limiting step in the formation of 2,3-BDO is its conversion from acetoin to 2,3-BDO by the enzyme butanediol dehydrogenase (2,3-BDH). Such 2,3-BDHs were screened based on higher affinity (lower Km) towards acetoin as substrate. The in silico docking studies were conducted for further validation, and they showed a high interaction profile for the PpBDH protein towards acetoin. Heterologous expression of these genes was studied in engineered Bacillus subtilis (BS1A1). In this study, it was seen that 2,3-BDH from Paenibacillus polymyxa ZJ-9 was reported to have higher enzyme activity levels, and in the fermentation studies, it was seen that the ratio of 2,3-BDO to acetoin was increased by 80.25%. The insights encourage further bioprocess optimization for increasing the fermentative production of 2,3-BDO. Our results provide a potential strategy to avoid the back conversion of 2,3-BDO to acetoin in an engineered Bacillus system.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"103 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140128697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient production of 1,3-propanediol from glycerol by a newly isolated soil bacterium using fed-batch fermentation 一种新分离的土壤细菌利用饲料批量发酵法从甘油中高效生产 1,3-丙二醇

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-12 DOI: 10.1007/s12257-024-00031-7

Selim Ashoor, Zhuang Yao, Chan Woo Song, Haeng Lim Lee, Hyeon Jeong Seong, Sampathkumar Palaniswamy, Jong Myoung Park, Hyohak Song, Yu-Sin Jang

引用次数: 0

Engineering potyvirus-like particles to display multiple copies of tuberculosis antigens 设计出显示多份结核病抗原的壶状病毒颗粒

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-11 DOI: 10.1007/s12257-024-00089-3

R. Princess, M. L. Stephen Raj

{"title":"Engineering potyvirus-like particles to display multiple copies of tuberculosis antigens","authors":"R. Princess, M. L. Stephen Raj","doi":"10.1007/s12257-024-00089-3","DOIUrl":"https://doi.org/10.1007/s12257-024-00089-3","url":null,"abstract":"Elicitation of antibody and cell-mediated immune responses are crucial for successful vaccine development against tuberculosis (TB). Mycobacterium tuberculosis (Mtb) antigens CFP10 and ESAT6, potent and proven vaccine candidates require appropriate adjuvants to trigger better immune response. Virus-like particles carrying repetitive copies of foreign antigens can induce both T and B cell-mediated immunity required for conferring protection against intracellular pathogens. In this study, we developed hybrid potyvirus-like particles (PVLPs) displaying mycobacterial antigens on their surface by translationally fusing the coat protein (CP) gene derived from Johnson grass mosaic virus with CFP 10 or/and ESAT 6 gene(s). The recombinant plasmids carrying fusion constructs were transformed into Escherichia coli, the fusion proteins, viz. ESAT6-CP, CP-CFP10 and ESAT6-CP-CFP10, were expressed and purified using Ni-NTA2+ affinity chromatography under denaturing conditions. The chimeric CP fusion proteins were self-assembled in vitro into PVLPs by the gradual removal of denaturing conditions. The purified hybrid PVLPs carrying Mtb antigens when injected into mice showed enhanced immunogenicity for both ESAT6 and CFP10 antigens compared to the same antigens immunized without any adjuvant. In vitro stimulation of splenocytes derived from mice immunized with chimeric PVLPs upregulates the expression of cytokines involved in TB immune response.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"366 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140128767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioanalysis of ursodeoxycholic acid and its metabolites and improved oral bioavailability using mixed micelles with poloxamer 407 and polysorbate 80 熊去氧胆酸及其代谢物的生物分析以及使用聚氧乙烯酰胺 407 和聚山梨醇酯 80 混合胶束提高口服生物利用度

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-11 DOI: 10.1007/s12257-024-00064-y

So Yeon Jeon, Chul Haeng Lee, Jihoon Lee, Ji-Hyeon Jeon, Yelim Jin, Im-Sook Song, Min-Koo Choi

{"title":"Bioanalysis of ursodeoxycholic acid and its metabolites and improved oral bioavailability using mixed micelles with poloxamer 407 and polysorbate 80","authors":"So Yeon Jeon, Chul Haeng Lee, Jihoon Lee, Ji-Hyeon Jeon, Yelim Jin, Im-Sook Song, Min-Koo Choi","doi":"10.1007/s12257-024-00064-y","DOIUrl":"https://doi.org/10.1007/s12257-024-00064-y","url":null,"abstract":"The development of analytical methods for endogenous therapeutic substances is a critical but challenging issue as obtaining a blank matrix without endogenous substance is impossible. To address this issue, we prepared a surrogate biological matrix by removing endogenous bile acids from rat plasma using a charcoal-stripped method and developed an analytical method for ursodeoxycholic acid (UDCA) and its conjugated metabolites, tauroursodeoxycholic (TUDCA) and glycoursodeoxycholic acid (GUDCA), including the use of surrogate matrices and protein precipitation method. In addition, we applied the bioanalytical method to investigate the bioavailability of UDCA-mixed micelle powder formulation (UDCA-MM). The oral bioavailability of UDCA in rats was calculated as 15.2% and increased 3.32-fold following the oral administration of UDCA-MM with the increased production of TUDCA without significant change in GUDCA. The UDCA-MM powder was prepared by thin-layer hydration and subsequent freeze-drying method in a ratio of UDCA/polysorbate 80/poloxamer 407 = 1:1:10 (w/w/w). The UDCA-MM was easily dispersed with a particle size of 16.5 ± 2.2 nm and solubility of 1120 ± 38 μg/mL, which represented a 175.3-fold increase in its solubility of UDCA. In conclusion, we developed and validated a simple and reliable bioanalytical method for UDCA, TUDCA, and GUDCA using the charcoal-stripped plasma as surrogate matrices. Our bioanalytical method successfully supported the assessment of the pharmacokinetics or bioavailability of UDCA, TUDCA, and GUDCA after the intravenous or oral dosing of UDCA and UDCA-MM. The UDCA-MM using poloxamer 407 and polysorbate 80 is a promising technique for increasing the solubility and oral absorption of UDCA.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140097260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0