Photothermal heating of cell-free reactions for on-site production of recombinant proteins

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Kyunghwan Yeom, Yu Jin Park, Hansol Kim, Dong-Yeon Song, Dong-Myung Kim, Ji-Ho Park
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Abstract

Cell-free synthesis technology is emerging as a versatile platform for biomanufacturing, particularly for on-site production of essential products. In this study, we address a pivotal challenge encountered when utilizing cell-free protein synthesis (CFPS) for on-site production in resource-limited settings. The efficacy of CFPS critically depends on precise temperature regulation. However, traditional heating methods are impractical for application outside of laboratory environments. To address this challenge, we propose an innovative solution that integrates gold nanorods (GNRs) with photothermal properties into the CFPS reaction mixture. Upon activation by a handheld laser module emitting near-infrared light, these GNRs efficiently convert light energy into heat, enabling rapid and precise temperature control for protein synthesis. Our approach not only achieves optimal synthesis temperatures more rapidly than conventional methods but also significantly enhances protein yields, with an increase of over threefold within a 30-min reaction period. In summary, our findings highlight the potential of photothermal heating as a means to ensure portable and efficient protein synthesis in the field. This advancement brings CFPS closer to achieving real-time, on-site protein production.

Abstract Image

现场生产重组蛋白质的无细胞反应光热加热技术
无细胞合成技术正在成为生物制造的多功能平台,特别是用于现场生产必需品。在这项研究中,我们探讨了在资源有限的环境中利用无细胞蛋白质合成(CFPS)进行现场生产时遇到的一个关键挑战。无细胞蛋白质合成的功效关键取决于精确的温度调节。然而,传统的加热方法不适合在实验室环境以外的地方应用。为了应对这一挑战,我们提出了一种创新解决方案,将具有光热特性的金纳米棒(GNRs)集成到 CFPS 反应混合物中。当手持激光模块发出近红外线激活这些 GNRs 时,这些 GNRs 能有效地将光能转化为热能,从而实现蛋白质合成的快速、精确的温度控制。与传统方法相比,我们的方法不仅能更快地达到最佳合成温度,还能显著提高蛋白质产量,在 30 分钟的反应时间内,蛋白质产量提高了三倍多。总之,我们的研究结果凸显了光热加热作为确保现场便携式高效蛋白质合成的一种手段的潜力。这一进步使 CFPS 离实现实时、现场蛋白质生产更近了一步。
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来源期刊
Biotechnology and Bioprocess Engineering
Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物
CiteScore
5.00
自引率
12.50%
发文量
79
审稿时长
3 months
期刊介绍: Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
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