Biotechnology and Bioprocess Engineering最新文献

Biocatalytic syn-stereoselective synthesis of alkyloxime, oxime ether, and their derivatives 烷基氧肟、肟醚及其衍生物的生物催化立体选择性合成

4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2025-11-21 DOI: 10.1007/s12257-025-00238-2

Taimur Khan, Khan Bahadar, Maaz Khan, Gao Hanzi, Muhammad Shahab, Guojun Zheng

引用次数: 0

Exploration of novel salt-tolerant β-galactosidases for GOS synthesis based on whole genome sequencing of Bacillus hwajinpoensis Zjut HJ2101 基于芽孢杆菌Zjut HJ2101全基因组测序的新型GOS合成耐盐β-半乳糖苷酶的探索

4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2025-11-05 DOI: 10.1007/s12257-025-00231-9

Changsheng Zhong, Lihong Liu, Wenrui Ding, Haoran Lin, Jin Huang

引用次数: 0

Evaluation of mitochondrial activity via cellular interactions between adrenal and neuronal cells in a microfluidic coculture device 在微流体共培养装置中，通过肾上腺细胞和神经元细胞之间的细胞相互作用评估线粒体活性

4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2025-04-16 DOI: 10.1007/s12257-025-00194-x

Jae Seong Kim, Huiseong Son, Jihun Han, Hanvit Cha, Jin Hyup Lee, Seung Pil Pack, Chang‐Soo Lee

引用次数: 4

Inhibiting angiogenesis and osteogenesis by targeted regulation of KLK4 via the exosomal MiR-422a derived from bone mesenchymal stem cells 通过来自骨间充质干细胞的外泌体MiR-422a靶向调控KLK4抑制血管生成和成骨

4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2025-03-04 DOI: 10.1007/s12257-024-00168-5

Qifei He, Qianzheng Zhuang, Zhaoqiang Deng, Haibin Wang, Jiaji Yue, Luoyong Jiang, Wei You, Wei Sun, Wei Xie

引用次数: 1

Assessing the applicability of tunicate skin-extracted cellulose as a base material for ultrasound gel 评估将鳞毛皮提取的纤维素作为超声凝胶基质材料的适用性

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-09-19 DOI: 10.1007/s12257-024-00146-x

Ji Woo Han, Nu Ri Han, Hye Jin Hwang, Byung Man Lee, Hwa Sung Shin, Sang Hyun Lee, Yun Jung Yang

{"title":"Assessing the applicability of tunicate skin-extracted cellulose as a base material for ultrasound gel","authors":"Ji Woo Han, Nu Ri Han, Hye Jin Hwang, Byung Man Lee, Hwa Sung Shin, Sang Hyun Lee, Yun Jung Yang","doi":"10.1007/s12257-024-00146-x","DOIUrl":"https://doi.org/10.1007/s12257-024-00146-x","url":null,"abstract":"Cellulose is widely considered an outstanding biomaterial due to its remarkable ionic properties, exceptional biocompatibility, and low toxicity. Its abundant surface hydroxyl groups facilitate increased hydrogen bonding, improving gelation and swelling capabilities. Moreover, incorporating carboxymethyl groups enhances solubility and allows for diverse formulations, serving as multifunctional cross-linkers. Among the various sources of this compound, tunicate-derived cellulose is an animal-derived cellulose and food byproduct with low utility. However, recycling tunicate skin into a useful biomaterial would provide access to the unique characteristics of animal cellulose, distinct from those of plant-derived cellulose. Particularly, tunicate cellulose has a longer fiber length than plant cellulose, enhancing the sound propagation speed within the material and making it suitable for the production of ultrasound-responsive gels. This study examined the viscosity and conductivity of tunicate-derived carboxymethyl celluloseto assess its applicability as an ultrasound gel. Additionally, small molecule release after ultrasound stimulation was also evaluated.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"22 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of protein–inorganic biohybrid as an imageable drug delivery system comprising transferrin, green fluorescent protein, and copper phosphate 制作蛋白质-无机生物杂化物，作为由转铁蛋白、绿色荧光蛋白和磷酸铜组成的可成像给药系统

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-09-17 DOI: 10.1007/s12257-024-00148-9

Seung Woo Lee, Yoojin Choi, Yeong Hyeock Kim, Jeong Eun Ham, Suresh Kumar Kailasa, Tae Jung Park

{"title":"Fabrication of protein–inorganic biohybrid as an imageable drug delivery system comprising transferrin, green fluorescent protein, and copper phosphate","authors":"Seung Woo Lee, Yoojin Choi, Yeong Hyeock Kim, Jeong Eun Ham, Suresh Kumar Kailasa, Tae Jung Park","doi":"10.1007/s12257-024-00148-9","DOIUrl":"https://doi.org/10.1007/s12257-024-00148-9","url":null,"abstract":"Organic–inorganic biohybrids have recently garnered attention for biomedical applications owing to their outstanding catalytic activity and biocompatibility. However, their efficacy in enhancing specificity toward drug targets remains limited. Here, we developed a transferrin–doxorubicin (TRF–DOX) complex and green fluorescence protein (GFP)-conjugated copper (Cu) phosphate (TRF–DOX@GFP@Cu biohybrid) for use as an imageable drug delivery system (DDS). TRF was utilized to increase the affinity of drug carriers for TRF receptors on cancer cells, and DOX was selected as a model drug. Additionally, GFP provides fluorescence properties for bioimaging and Cu ions serve as the skeleton for forming the flower-shaped inorganic material. By adjusting the concentrations of TRF–DOX and GFP with 25 mg mL−1 of Cu precursors, six flower-shaped TRF–DOX@GFP@Cu biohybrids were fabricated. Among these, biohybrid-5 (prepared using 0.05 mg mL−1 TRF–DOX and 0.10 mg mL−1 of GFP with 25 mg mL−1 of Cu ions) exhibited the strongest fluorescence. We characterized the morphology, composition, functional groups, and specific surface area of the TRF–DOX@GFP@Cu biohybrid. Biohybrid-5 had a specific surface area of 37.508 m2 g−1 and could effectively bind to A549 lung cancer cells as shown by fluorescence imaging. The novel TRF–DOX@GFP@Cu biohybrid fabricated in this study has potential as a DDS in the treatment of lung cancer.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"48 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continuous cell recycling in methylotrophic yeast Pichia pastoris to enhance product yields: a case study with Yarrowia lipolytica lipase Lip2 在养甲酵母 Pichia pastoris 中进行连续细胞循环以提高产品产量：利用 Yarrowia lipolytica 脂肪酶 Lip2 进行的案例研究

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-09-12 DOI: 10.1007/s12257-024-00149-8

Shilpa Mohanty, Babbal, Shivani Chauhan, Mohini Talwar, Yogender Pal Khasa

{"title":"Continuous cell recycling in methylotrophic yeast Pichia pastoris to enhance product yields: a case study with Yarrowia lipolytica lipase Lip2","authors":"Shilpa Mohanty, Babbal, Shivani Chauhan, Mohini Talwar, Yogender Pal Khasa","doi":"10.1007/s12257-024-00149-8","DOIUrl":"https://doi.org/10.1007/s12257-024-00149-8","url":null,"abstract":"A robust cell recycling strategy based on the methylotrophic yeast, Pichia pastoris, was established to enhance product titers of the commercially important Yarrowia lipolytica lipase Lip2. The expression of Lip2 protein in the prokaryotic host Escherichia coli resulted in inclusion bodies, whereas utilization of SUMO fusion tag could not improve its solubility. Therefore, Lip2 extracellular expression was targeted via the Saccharomyces cerevisiae α-mating signal sequence in P. pastoris under methanol-inducible AOX1 promoter. Shake flask expression studies of hyper-producer Pichia clone under optimized conditions resulted in 438.83 and 420.09 mg/L of glycosylated Lip2 production after induction at an OD600 of 10 and 20, respectively. A high Lip2 productivity was further targeted using a cell retention technique where the cell biomass was recycled to obtain higher product concentration with improved product quality. The biomass recycling at every 72 h followed a 3.8-fold enhanced Lip2 concentration with a cumulative volumetric product concentration of 1,794 mg/L. A high specific product yield (YP/X) in the range of 37.45–47.00 mg/g dry cell weight (DCW) was also maintained up to five retention cycles. Furthermore, higher cumulative protein yields were obtained from the 5-time recycled cells compared to five individual batch runs at shake flask up to 72 h. High cell density cultivation of recombinant P. pastoris in a 2.5-L fermenter yielded 5.25 g/L of Lip2 enzyme with a maximum specific yield of 51.97 mg/g DCW.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"45 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142224688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitive detection of SARS-CoV2 spike antibodies by a paper-based polypyrrole/reduced graphene oxide sensor 用纸基聚吡咯/还原氧化石墨烯传感器灵敏检测 SARS-CoV2 穗状抗体

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-09-10 DOI: 10.1007/s12257-024-00150-1

Hsiao-Ming Chang, Yibing Zhang, Casey Hashimoto, Carlos I. Vazquez, Yile Fang, Parveen Kumar, Anand Gadre, Changqing Li, Wei-Chun Chin

{"title":"Sensitive detection of SARS-CoV2 spike antibodies by a paper-based polypyrrole/reduced graphene oxide sensor","authors":"Hsiao-Ming Chang, Yibing Zhang, Casey Hashimoto, Carlos I. Vazquez, Yile Fang, Parveen Kumar, Anand Gadre, Changqing Li, Wei-Chun Chin","doi":"10.1007/s12257-024-00150-1","DOIUrl":"https://doi.org/10.1007/s12257-024-00150-1","url":null,"abstract":"The presence of antibodies (Abs) in the body is an indicator of the individual immune response to an infection. In the post-pandemic era, an accurate, cheap, and rapid antibiotic sensor is critically needed to monitor Ab levels. Therefore, we present a simple fabrication method for a highly sensitive electrochemical sensor that detects SARS-CoV2 spike Abs. The sensor comprises filter paper with a composite made of polypyrrole (PPy) and reduced graphene oxide (rGO). The rGO structure is observed using a scanning electron microscope and Fourier-transform infrared spectra (FTIR). The presence of rGO improves protein conjugation which is identified using a fluorescent microscope and FTIR. The sensitivity test performs high selectivity and reproducibility in two different SARS-CoV2 Abs separately and shows the range of detection limits between 10 and 1000 fg/mL. The results demonstrate an advancement in developing PPy/rGO composite sensors for SARS-CoV2 Ab detection and as a promising sensor for monitoring COVID-19 exposures and infections.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"36 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A neural ordinary differential equation model for predicting the growth of Chinese Hamster Ovary cell in a bioreactor system 预测中国仓鼠卵巢细胞在生物反应器系统中生长的神经常微分方程模型

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-09-01 DOI: 10.1007/s12257-024-00141-2

Kuo-Chun Chiu, Dongping Du

{"title":"A neural ordinary differential equation model for predicting the growth of Chinese Hamster Ovary cell in a bioreactor system","authors":"Kuo-Chun Chiu, Dongping Du","doi":"10.1007/s12257-024-00141-2","DOIUrl":"https://doi.org/10.1007/s12257-024-00141-2","url":null,"abstract":"Chinese hamster ovary (CHO) cells play an important role in the biopharmaceutical industry, but their production efficiency requires enhancement to meet the growing market demands. Artificial intelligence (AI) has been a potent tool for modeling bioprocesses to support biopharmaceutical manufacturing. However, existing AI models do not adapt well to process data collected at irregular time intervals and have limited capability to scale up and down to incorporate various process parameters. To address the limitations, this study develops a novel neural ordinary differential equation (ODE) model for predicting key variables such as viable cell concentration, glucose concentration, lactate concentration, pH, and dissolved oxygen in a CHO cell bioreactor. Validated through extensive bioreactor experiments, the neural ODE model shows a better accuracy compared to the benchmark models, which include a conventional mechanistic model and a hybrid model. Additionally, the neural ODE model incorporated essential process variables that were not considered in the previous models. It successfully extrapolates to predict unknown dynamics at different initial conditions, which showcases robust adaptability. Moreover, the model provides useful insights into the relationship among variables, highlighting its potential for bioprocess modeling.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"36 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142224689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of two halophilic bacteria producing polyhydroxybutyrate from high-salt environment 从高盐环境中分离并鉴定两种产生聚羟基丁酸的嗜卤细菌

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-08-23 DOI: 10.1007/s12257-024-00140-3

Youjung Kong, Hyun Gi Koh, Haeng-Geun Cha, Byung Wook Lee, Kyungjae Yu, See-Hyoung Park, Kyungmoon Park

{"title":"Isolation and characterization of two halophilic bacteria producing polyhydroxybutyrate from high-salt environment","authors":"Youjung Kong, Hyun Gi Koh, Haeng-Geun Cha, Byung Wook Lee, Kyungjae Yu, See-Hyoung Park, Kyungmoon Park","doi":"10.1007/s12257-024-00140-3","DOIUrl":"https://doi.org/10.1007/s12257-024-00140-3","url":null,"abstract":"Polyhydroxyalkanoates (PHAs), one of biodegradable polyesters, are substances that store carbon and energy in various microorganisms. Polyhydroxybutyrate (PHB) is the most commonly type of PHAs with relatively simple chemical structure. In this study, we developed a polymerase chain reaction (PCR) screening method for screen novel halophilic bacteria containing PHA synthase (PhaC) gene. Halophilic bacteria were collected and isolated from high-salt environment such as sea soil or shrimp jeotgal in South Korea. Primer set was designed based on the conserved region in Class I PhaC from 30 kinds of Halomonas species. The designed primer set was used to optimize PCR conditions to identify PhaC gene in newly isolated 15 halophilic bacteria from sea soil or shrimp jeotgal. Among 15 candidates, five bacteria were selected after PCR and agarose gel analysis and confirmed to produce PHB. Two bacteria with higher PHB production were identified as Halomonas and Marinobacter sp. by 16 s rRNA analysis and named as Halomonas shrimpha IBTH01 and Marinobacter haeunpha IBTM02. Then, PHB production was examined by changing culture temperature, media composition, carbon source, and glucose concentration. Finally, PHB from two bacteria was analyzed by transmission electron microscopy, gas chromatography, and 1H nuclear magnetic resonance. Taken together, this study will contribute to establish a platform for the utilization of novel halophilic bacteria in the synthesis of PHA polymers.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"13 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0