Fabrication of protein–inorganic biohybrid as an imageable drug delivery system comprising transferrin, green fluorescent protein, and copper phosphate
IF 2.5 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Seung Woo Lee, Yoojin Choi, Yeong Hyeock Kim, Jeong Eun Ham, Suresh Kumar Kailasa, Tae Jung Park
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引用次数: 0
Abstract
Organic–inorganic biohybrids have recently garnered attention for biomedical applications owing to their outstanding catalytic activity and biocompatibility. However, their efficacy in enhancing specificity toward drug targets remains limited. Here, we developed a transferrin–doxorubicin (TRF–DOX) complex and green fluorescence protein (GFP)-conjugated copper (Cu) phosphate (TRF–DOX@GFP@Cu biohybrid) for use as an imageable drug delivery system (DDS). TRF was utilized to increase the affinity of drug carriers for TRF receptors on cancer cells, and DOX was selected as a model drug. Additionally, GFP provides fluorescence properties for bioimaging and Cu ions serve as the skeleton for forming the flower-shaped inorganic material. By adjusting the concentrations of TRF–DOX and GFP with 25 mg mL−1 of Cu precursors, six flower-shaped TRF–DOX@GFP@Cu biohybrids were fabricated. Among these, biohybrid-5 (prepared using 0.05 mg mL−1 TRF–DOX and 0.10 mg mL−1 of GFP with 25 mg mL−1 of Cu ions) exhibited the strongest fluorescence. We characterized the morphology, composition, functional groups, and specific surface area of the TRF–DOX@GFP@Cu biohybrid. Biohybrid-5 had a specific surface area of 37.508 m2 g−1 and could effectively bind to A549 lung cancer cells as shown by fluorescence imaging. The novel TRF–DOX@GFP@Cu biohybrid fabricated in this study has potential as a DDS in the treatment of lung cancer.
期刊介绍:
Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.