Biotechnology and Bioprocess Engineering最新文献_第2页

Nano-sized lysate of Lactiplantibacillus plantarum isolated from green tea leaves as a potential skin care ingredient 从绿茶叶中分离出的植物乳杆菌纳米级裂解物作为一种潜在的护肤成分

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-08-21 DOI: 10.1007/s12257-024-00132-3

Kilsun Myoung, Eun-Jeong Choi, Sehyun Kim, Jeong Ah Hwang, Ji Young Lee, Hyoung-June Kim, Jae Sung Hwang

{"title":"Nano-sized lysate of Lactiplantibacillus plantarum isolated from green tea leaves as a potential skin care ingredient","authors":"Kilsun Myoung, Eun-Jeong Choi, Sehyun Kim, Jeong Ah Hwang, Ji Young Lee, Hyoung-June Kim, Jae Sung Hwang","doi":"10.1007/s12257-024-00132-3","DOIUrl":"https://doi.org/10.1007/s12257-024-00132-3","url":null,"abstract":"Fermentation products and lysates of lactic acid bacteria (LAB) have been developed as cosmetic ingredients. The topical application of certain LAB strains can improve skin health and combat skin diseases. Here, we investigated the effects of nano-sized lysate of Lactiplantibacillus plantarum APsulloc 331261 (NLAP) isolated from green tea leaves on human skin cells and a reconstructed human epidermis (RHE) model. NLAP increased the expression of genes involved in skin barrier functions such as proliferation, differentiation, tight junction formation, and antimicrobial peptides in normal human epidermal keratinocytes. NLAP prevented the decrease in the expression of differentiation markers and increased release of inflammatory cytokines in keratinocytes cultured with Staphylococcus aureus. NLAP-induced improvements in gene expression and cytokine levels were also observed in RHE treated with heat-killed S. aureus. Additionally, the skin barrier-strengthening effect of NLAP was confirmed by comparing the penetration of the fluorescent dye into the RHE. These findings suggest that NLAP could aid skin barrier function, protect the skin against detrimental bacteria, and suppress inflammatory responses; thus, it can be developed as a skincare ingredient.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"47 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-production of biosurfactant and indigo using wild-type Acinetobacter sp. isolated from soil 利用从土壤中分离出来的野生型醋酸杆菌共同生产生物表面活性剂和靛蓝

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-08-20 DOI: 10.1007/s12257-024-00143-0

Chan-Seo Yeo, Pammidimarri D. V. N. Sudheer, Kwon-Young Choi

{"title":"Co-production of biosurfactant and indigo using wild-type Acinetobacter sp. isolated from soil","authors":"Chan-Seo Yeo, Pammidimarri D. V. N. Sudheer, Kwon-Young Choi","doi":"10.1007/s12257-024-00143-0","DOIUrl":"https://doi.org/10.1007/s12257-024-00143-0","url":null,"abstract":"In this research, we investigated a naturally occurring, non-genetically modified strain of Acinetobacter sp., isolated from soil, which demonstrated the capability to produce both indigo and biosurfactant. During the screening, indole was used as the sole carbon source in M9 minimal medium. The strain exhibiting the most intense blue coloration was isolated and further analyzed. The blue dye extracted from the cell culture was confirmed as indigo through LC/MS analysis, showing an m/z value of 263.5, and H-NMR analysis. In LB medium, the wild-type Acinetobacter sp. strain produced approximately 6.8 mg/L of indigo from 1 mM indole. However, in M9 minimal medium, the production yield significantly increased to 45.5 mg/L. Notably, the isolated strain showed vigorous bubbling during growth, which could facilitate the transport of indole and indigo dye, both of which have low solubility, across cell membranes. Additionally, this strain was capable of degrading medium-chain C12 alkane efficiently. The whole genome was fully sequenced and analyzed for genes concerning biosurfactant and alkane metabolisms. In conclusion, utilizing a wild-type strain for indigo production offers a promising alternative to traditional chemical processes, addressing concerns related to genetically modified organisms in future applications.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"39 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of a halotolerant poly(ε-caprolactone)-depolymerizing strain of Bacillus gibsonii from seaside soil 从海边土壤中分离出耐盐碱的聚(ε-己内酯)解聚菌株 Bacillus gibsonii

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-08-17 DOI: 10.1007/s12257-024-00133-2

Ki-Ryeon Kim, Jin-Wan Park, Eun-bi Cho, Young-Ah Jang, Gyeong Tae Eom, Yu-Ri Oh

{"title":"Isolation of a halotolerant poly(ε-caprolactone)-depolymerizing strain of Bacillus gibsonii from seaside soil","authors":"Ki-Ryeon Kim, Jin-Wan Park, Eun-bi Cho, Young-Ah Jang, Gyeong Tae Eom, Yu-Ri Oh","doi":"10.1007/s12257-024-00133-2","DOIUrl":"https://doi.org/10.1007/s12257-024-00133-2","url":null,"abstract":"Few studies have investigated the biodegradation of microplastics in marine environments. Microorganisms that can degrade microplastics in high-salinity conditions are sought after. Therefore, we aimed to isolate a halotolerant poly(ε-caprolactone) (PCL)-degrading bacterium for applications in biotechnology. The bacterium isolated from seaside soil was identified as Bacillus gibsonii via phylogenetic analysis based on 16S rRNA gene sequences and designated as KRICT-1. We tested whether the KRICT-1 strain showed halotolerance by determining the sodium chloride (NaCl) tolerance at various concentrations. The KRICT-1 strain showed growth at up to 10% NaCl on Luria–Bertani (LB) medium agar plates and 10% NaCl in liquid LB medium, indicating that KRICT-1 can grow and produce a PCL-depolymerizing enzyme under high-salt conditions. The KRICT-1 strain could depolymerize PCL with a PCL film weight loss of 2.82% at up to 10% NaCl concentration after cultivation of 7 weeks. KRICT-1 is the first strain of B. gibsonii which shows PCL-depolymerizing activity. Scanning electron microscopy and water contact angle results confirmed the degradation of PCL by the KRICT-1 strain. The extracellular enzyme produced by the KRICT-1 strain was stable over a wide range of temperatures (15–40 °C) and pH (7.0–9.5). This halotolerant PCL-degrading bacterium can be used in the degradation of biodegradable plastics present in saline soils, saline water, and wastewater.<h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>\u0000","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"9 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of anticancer targets for triple-negative breast cancer through comparative analysis of gene dependency score 通过基因依赖性评分比较分析发现三阴性乳腺癌的抗癌靶点

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-08-14 DOI: 10.1007/s12257-024-00138-x

Bo Kyung Kim, Gahee Kim, Wonhee Hur, Yoojin Choi, Suhyun Hwangbo, Jae Yong Ryu

{"title":"Discovery of anticancer targets for triple-negative breast cancer through comparative analysis of gene dependency score","authors":"Bo Kyung Kim, Gahee Kim, Wonhee Hur, Yoojin Choi, Suhyun Hwangbo, Jae Yong Ryu","doi":"10.1007/s12257-024-00138-x","DOIUrl":"https://doi.org/10.1007/s12257-024-00138-x","url":null,"abstract":"Patients with triple-negative breast cancer (TNBC) often face an unfavorable prognosis due to the lack of targeted therapy. Thus, identifying drug targets specific to the TNBC subtype is crucial for effective treatment. Here, we propose a strategy to identify potential inhibitory targets specific to this subtype based on the gene dependency score (GDS), a quantitative measure of essentiality of each gene determined in cancer cell lines. Specifically, we compared the GDS values of 17,387 genes among cell lines of four breast cancer (BC) subtypes, namely luminal A, luminal B, HER2-positive, and TNBC, to identify genes showing specific essentiality in TNBC subtype cell lines. Twenty-two genes were predicted as potential inhibitory targets. Of these, we selected two genes, ILK and RHOA, based on survival analysis conducted across the four BC subtypes. We propose these two genes as potential biomarkers for TNBC. Furthermore, we experimentally validated that inhibiting ILK expression with a specific inhibitor reduced cell viability more in TNBC subtype cell lines than in other BC subtype cell lines. Therefore, ILK is a potential drug target specific to TNBC. The strategy proposed is expected to be useful in identifying biomarker and therapeutic target genes in not only BC but also other cancers.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"29 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Promiscuous acylase as a green catalyst: to directly catalyze the conjugate addition reaction for the synthesis of brivaracetam intermediates 作为绿色催化剂的杂合酰化酶：直接催化共轭加成反应以合成双呋喃乙酰胺中间体

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-08-13 DOI: 10.1007/s12257-024-00135-0

Taimur Khan, Daixi Wang, Muhammad Shahab, Qaim Ali, Guojun Zheng

{"title":"Promiscuous acylase as a green catalyst: to directly catalyze the conjugate addition reaction for the synthesis of brivaracetam intermediates","authors":"Taimur Khan, Daixi Wang, Muhammad Shahab, Qaim Ali, Guojun Zheng","doi":"10.1007/s12257-024-00135-0","DOIUrl":"https://doi.org/10.1007/s12257-024-00135-0","url":null,"abstract":"Epilepsy, a predominant neurological disorder affecting about 1% of the worldwide population, demands effective treatment options. An antiepileptic drug called brivaracetam has proven amazing efficacy in preventing epilepsy progression, garnering attention for novel synthesis methods. Despite recent progress in conventional synthesis routes, challenges such as expensive catalysts, inconvenient substrates, and hazardous solvents persist. In this context, we share the first finding that immobilized penicillin G acylase (IPGA) can catalyze the polarity reversal conjugate addition reaction. This synthesis is straightforward and does not require any purification. Yield up to 92.41% was achieved at 55 °C using dimethyl sulfoxide as a solvent. The catalytic specificity of IPGA was demonstrated through control experiments. Nonetheless, this research demonstrates the potential of IPGA and other biocatalysts to enable sustainable and effective organic synthesis processes and showcase the promiscuity of existing enzymes.<h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"7 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Event-specific loop-mediated isothermal amplification for living modified cotton MON88701, MON531, MON15985, MON88913, and COT102 针对活体改良棉花 MON88701、MON531、MON15985、MON88913 和 COT102 的事件特异性环介导等温扩增技术

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-08-12 DOI: 10.1007/s12257-024-00137-y

Wonkyun Choi, A-Mi Yoon, Jun-Woo Lee, Hye Song Lim, Young Jun Jung, Jung Ro Lee

{"title":"Event-specific loop-mediated isothermal amplification for living modified cotton MON88701, MON531, MON15985, MON88913, and COT102","authors":"Wonkyun Choi, A-Mi Yoon, Jun-Woo Lee, Hye Song Lim, Young Jun Jung, Jung Ro Lee","doi":"10.1007/s12257-024-00137-y","DOIUrl":"https://doi.org/10.1007/s12257-024-00137-y","url":null,"abstract":"Gossypium hirsutum L., commonly known as upland cotton, is cultivated globally for natural fiber, feed, and seed oil. To enhance agricultural productivity and quality, living modified (LM) cotton has been developed and utilized since the late 1990s. Due to environmental concerns, such as biodiversity risks associated with living modified organisms (LMOs), the Korean government, including the Ministry of Environment, has been conducting LMO natural environment monitoring and post-management projects. In this study, we developed a loop-mediated isothermal amplification (LAMP) assay to detect five specific LM cotton events (MON88701, MON531, MON15985, MON88913, and COT102), which were the most abundant volunteers observed from 2019 to 2021 in South Korea. The event-specific LAMP assays for the five LM cotton events were established with a 40-min reaction time using LAMP reaction buffer, Bst DNA polymerase, and event-specific primers. The limit of detection ranged from 0.01 to 1 ng/μL. The assays' specificity and sensitivity were validated through colorimetric changes, fluorescence intensity measurements, and conventional PCR. To demonstrate the practical application of the LAMP assays, we tested 22 LM cotton volunteers collected from the natural environment in 2021. Each LAMP assay event-specifically amplified the respective LM cotton volunteers. These results indicate that the developed LAMP assays are effective tools for the efficient management and detection of LM cotton in field surveys, supporting regulatory compliance and environmental monitoring efforts.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"30 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141946152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of azo dye Reactive Black 5 by strict anaerobe Clostridium acetobutylicum and evaluation of its effects for hydrogen production 严格厌氧菌乙酰丁酸梭菌对偶氮染料活性黑 5 的生物降解及其制氢效果评估

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-08-01 DOI: 10.1007/s12257-024-00131-4

Hyun-Joong Kim, Suwon Kim, Jeong Hyeon Hwang, Yeda Lee, Yuni Shin, Suhye Choi, Jinok Oh, Joonseok Koh, Sang Ho Lee, Yong-Keun Choi, Shashi Kant Bhatia, Yung-Hun Yang

{"title":"Biodegradation of azo dye Reactive Black 5 by strict anaerobe Clostridium acetobutylicum and evaluation of its effects for hydrogen production","authors":"Hyun-Joong Kim, Suwon Kim, Jeong Hyeon Hwang, Yeda Lee, Yuni Shin, Suhye Choi, Jinok Oh, Joonseok Koh, Sang Ho Lee, Yong-Keun Choi, Shashi Kant Bhatia, Yung-Hun Yang","doi":"10.1007/s12257-024-00131-4","DOIUrl":"https://doi.org/10.1007/s12257-024-00131-4","url":null,"abstract":"As azo dyes are commercially used to treat textiles and leather articles, but they were toxic, allergenic, carcinogenic, and mutagenic to human and environment if they were not well treated, the content and degradation of azo dyes in wastewater were very important. So far, various methods such as physical, chemical, and biological methods have been applied to solve this. As a sequential work, we tried to remove a famous azo dye, Reactive Black 5 (RB5) by biological assay and found that Clostridium acetobutylicum showed the best decolorization of RB5 among the hydrogen producing Clostridium species examined. It was also found 100 ppm concentration of RB5 did not affect biohydrogen production of C. acetobutylicum suggesting simultaneous degradation of azo dye and biohydrogen production was possible. Optimal condition for simultaneous decolorization and biohydrogen production was selected as pH 6, 40 °C, and 200 rpm, and high concentration (200 ppm) of RB5 could be decolorized up to 97%. When C. acetobutylicum was examined for further decoloring activities, it was showed the decolorization of various dyes such as Bromophenol, Bromocresol, Methyl Red, and Reactive Red 120. This study showed potential application of C. acetobutylicum in wastewater treatment by simultaneous decolorizing and biohydrogen production.<h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>\u0000","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"180 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141881698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carcinoembryonic antigen-positive circulating epithelial cells as a biomarker for the diagnosis and prognosis of colorectal cancer 癌胚抗原阳性循环上皮细胞作为结直肠癌诊断和预后的生物标记物

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-08-01 DOI: 10.1007/s12257-024-00115-4

Hong Jae Jeon, Jin hyuk Seo, Euna Jeong, Chae Yeon Son, Piper A. Rawding, Yuri Hwang, Seha Bang, Tae min Jang, Luke J. Kubiatowicz, Sung Hee Hyun, Seungpyo Hong, Ik-Chan Song, Tae Hee Lee, Jiyoon Bu, Hyuk Soo Eun

{"title":"Carcinoembryonic antigen-positive circulating epithelial cells as a biomarker for the diagnosis and prognosis of colorectal cancer","authors":"Hong Jae Jeon, Jin hyuk Seo, Euna Jeong, Chae Yeon Son, Piper A. Rawding, Yuri Hwang, Seha Bang, Tae min Jang, Luke J. Kubiatowicz, Sung Hee Hyun, Seungpyo Hong, Ik-Chan Song, Tae Hee Lee, Jiyoon Bu, Hyuk Soo Eun","doi":"10.1007/s12257-024-00115-4","DOIUrl":"https://doi.org/10.1007/s12257-024-00115-4","url":null,"abstract":"The serum carcinoembryonic antigen (CEA) level lacks the specificity required for precise colorectal cancer (CRC) diagnosis. To address this limitation, we investigated CEA-expressing circulating epithelial cells (CCECs) as a diagnostic biomarker for CRC. Human peripheral blood mononuclear cells, obtained from patient samples, were suspended in Matrigel and subjected to cyto-centrifugation for immunohistochemistry staining, enhancing cell immobilization on glass slides. Using this method, we successfully identified CCECs in 63% of CRC patients, whereas they were present in only 5% of non-cancer cohorts. Subsequently, receiver operating characteristic (ROC) analysis demonstrated the superior diagnostic capability of the CCEC count (area under the curve [AUC]-ROC: 0.817; p = 0.003) compared to serum CEA levels (AUC-ROC: 0.747; p = 0.021). Furthermore, a strong correlation emerged between tissue CEA expression and CCEC counts, with CCECs exclusively detectable in patients with CEA-positive tumors. The CCEC count also exhibited a high predictive value for estimating lymphovascular invasion and distant metastasis. To further enhance the clinical utility of CCECs, we established an integrated CEA score by combining serum CEA levels and CCEC counts. This scoring system exhibited superior AUC-ROCs for distinguishing malignancy and estimating tumor metastatic potential compared to individual biomarkers. Our study underscores the promise of CCECs as a biomarker for distinguishing and diagnosing CRC.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"149 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141881696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioconjugated zinc oxide–quercetin nanocomposite enhances the selectivity and anti-biofilm activity of ZnO nanoparticles against Staphylococcus species 生物共轭氧化锌-槲皮素纳米复合材料增强了氧化锌纳米粒子对葡萄球菌的选择性和抗生物膜活性

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-07-30 DOI: 10.1007/s12257-024-00136-z

Hyemin Choi, Srimathi Raghavan, Joonho Shin, Jisung Kim, Kwang-sun Kim

{"title":"Bioconjugated zinc oxide–quercetin nanocomposite enhances the selectivity and anti-biofilm activity of ZnO nanoparticles against Staphylococcus species","authors":"Hyemin Choi, Srimathi Raghavan, Joonho Shin, Jisung Kim, Kwang-sun Kim","doi":"10.1007/s12257-024-00136-z","DOIUrl":"https://doi.org/10.1007/s12257-024-00136-z","url":null,"abstract":"Quercetin (Q), a plant-derived flavonoid, has antibacterial properties against a wide range of bacterial types, and preferentially targets gram-positive bacteria. However, because of its lower activity than that of current antibiotics, Q has only been used as an antimicrobial adjuvant, particularly against antimicrobial-resistant strains, where it works synergistically with other agents to increase their antimicrobial activity and selectivity. Zinc oxide (ZnO) nanomaterials, which are among the most extensively researched and powerful antibacterial compounds, have limitations in terms of off-target effects and cytotoxicity at high concentrations and this necessitates the use of biocompatible chemicals for their modification. In this study, we bio-conjugated ZnO with Q using cell-free supernatant of Staphylococcus ATCC 25923. UV–visible spectroscopy, X-ray diffraction analysis, Fourier-transform infrared spectroscopy, dynamic light scattering, and transmission electron microscopy confirmed the formation of ZnO nanoparticles (NPs) with Q nanocomposite (ZnO@Q NC). The in vitro antibacterial activity of ZnO@Q NC against gram-negative and gram-positive bacterial species was evaluated as the minimum inhibitory concentration. The results demonstrated that ZnO@Q NC exhibited a 4–32-fold higher preferential activity toward Staphylococcus species than Q and ZnO NPs. Additional mechanistic studies revealed that ZnO@Q NC disrupted bacterial membranes and prevented biofilm formation. The biocompatibility of ZnO@Q NC with WI-38 cells was assessed, and the synergistic use of ZnO@Q NC with antibiotics was suggested to reduce its cytotoxicity. Overall, the results demonstrated that conjugating ZnO NPs with Q can significantly boost their bactericidal efficacy and selective pressure against Staphylococcus species.<h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>\u0000","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"45 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141866139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-inflammatory and skin barrier regulation of cyanin chloride in TNF-α/IL-17A/IFN-γ-induced HaCaT psoriasis model 氯化氰在 TNF-α/IL-17A/IFN-γ 诱导的 HaCaT 银屑病模型中的抗炎和皮肤屏障调节作用

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-07-23 DOI: 10.1007/s12257-024-00134-1

Min Ji Kim, Hui Su Chung, Yea Ju Han, Jeong min Cho, Dong won Kim, Hyung Seo Hwang

{"title":"Anti-inflammatory and skin barrier regulation of cyanin chloride in TNF-α/IL-17A/IFN-γ-induced HaCaT psoriasis model","authors":"Min Ji Kim, Hui Su Chung, Yea Ju Han, Jeong min Cho, Dong won Kim, Hyung Seo Hwang","doi":"10.1007/s12257-024-00134-1","DOIUrl":"https://doi.org/10.1007/s12257-024-00134-1","url":null,"abstract":"Cyanin chloride, one of the active ingredients in figs, is a glycoside made of two sugars linked to a cyanidin aglycone. Although many studies have reported on the skin efficacy of cyanidin aglycone, there have been few reports on human psoriasis to date. Therefore, we focused on excessive inflammation and loss of skin barrier function, which are the main characteristics of psoriasis, and verified the function of cyanin chloride on the psoriasis. Cyanine chloride removed DPPH and ABTS radicals in a concentration-dependent manner and significantly inhibited NO production in LPS-induced RAW264.7 cells as well as suppressed inflammatory cytokines such as iNOS, COX-2, IL-6, and IL-1α/β. Moreover, we used TNF-α/IL-17A/IFN-γ-induced HaCaT cells, a human skin cell model of psoriasis. Cyanin chloride significantly inhibited the mRNA expression of inflammatory cytokines, such as IL-1α, IL-1β, and IL-6, and chemokines CXCL8 and CCL20 in TNF-α/IL-17A/IFN-γ-induced HaCaT cells. Cyanin chloride significantly inhibited the phosphorylation of STAT3 transcription factor in a concentration-dependent manner, confirming the regulatory function of CCL20 chemokine. Finally, cyanin chloride significantly restored the TEER value in TNF-α/IL-17A/IFN-γ-induced HaCaT cells, confirming the effect of strengthening the skin barrier function. In addition, cyanin chloride increased the mRNA levels of filaggrin which is cornified envelope proteins of the epidermal layer, in a concentration-dependent manner in normal epidermal cells. Taken together, the above results suggest that cyanin chloride can be considered a natural candidate for improving psoriasis, an incurable skin disease, not only by lowering excessive skin inflammatory reactions but also by restoring the skin barrier.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"14 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141769516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0