Biotechnology and Bioprocess Engineering最新文献_第3页

Advancing biofoundry development: strategies and challenges 推进生物产业发展：战略与挑战

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-07-02 DOI: 10.1007/s12257-024-00121-6

Aporva Gupta, Seung-Goo Lee, Bong-Hyun Sung, Dae-Hee Lee, Byung-Kwan Cho, Dong-Myung Kim, Haseong Kim

引用次数: 0

Production of chitosan from Aspergillus niger and quantitative evaluation of the process using adapted analytical tools 利用黑曲霉生产壳聚糖，并利用适用的分析工具对生产过程进行定量评估

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-07-02 DOI: 10.1007/s12257-024-00124-3

S. Krake, C. Conzelmann, S. Heuer, M. Dyballa, S. Zibek, T. Hahn

{"title":"Production of chitosan from Aspergillus niger and quantitative evaluation of the process using adapted analytical tools","authors":"S. Krake, C. Conzelmann, S. Heuer, M. Dyballa, S. Zibek, T. Hahn","doi":"10.1007/s12257-024-00124-3","DOIUrl":"https://doi.org/10.1007/s12257-024-00124-3","url":null,"abstract":"The chitosan production process from fishery waste is already established in industrial scale, whereby fungal chitosan is produced in lower amounts. Since fungal chitosan could be isolated from under-valorized vegan streams while exhibiting slightly different characteristics, it has also potential for other applications. Within this publication, we focus on the chitosan production from Aspergillus niger. This study provides a detailed determination of the biomass composition, adapting and comparing different analytical tools, with special focus on the chitin and chitosan content. The major content of the dried biomass is composed of glucans (48.6 ± 1.4%), followed by proteins with an amount of 22.2 ± 0.7%. Chitin and chitosan provide 16.0 ± 0.8% of the biomass. Within our chitosan production studies, we compared the effect of different process strategies including steps as deproteinization (DP), acid extraction (AE), deacetylation (DA), as well as purification. Initially, we obtained poor values (lower than 73.6%) for the chitosan purity. A direct DA step followed by purification resulted in a chitosan purity of up to 89.6%, a recovery of 30.5% and a yield with regard to the biomass of 5.5%. The DA degree of the resulting chitosan is similar to chitosan derived from fishery waste, whereas the molecular weight is lower. The results achieved so far are consistent with the literature, extending beyond, the data emphasized that a chitosan production from residual fungal biomass after fermentation is suitable by direct DA and purification. However, further adaption is necessary so that other matrix compounds could be also obtained.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"146 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141529697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic modeling of microorganisms involved in anaerobic digestion 厌氧消化微生物的代谢模型

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-07-01 DOI: 10.1007/s12257-024-00128-z

Junkyu Lee, Byung Tae Lee, Mun Su Kwon, Hyun Uk Kim

引用次数: 0

Primer generation-rolling circle amplification method optimized for the detection of pathogenic bacteria 用于检测病原菌的引物生成-滚动圈扩增优化方法

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-06-28 DOI: 10.1007/s12257-024-00117-2

Eun-Jin Jang, Tai-Yong Kim, Jeong-A. Lim, Min-Ah Woo

{"title":"Primer generation-rolling circle amplification method optimized for the detection of pathogenic bacteria","authors":"Eun-Jin Jang, Tai-Yong Kim, Jeong-A. Lim, Min-Ah Woo","doi":"10.1007/s12257-024-00117-2","DOIUrl":"https://doi.org/10.1007/s12257-024-00117-2","url":null,"abstract":"With advancements in DNA amplification research, isothermal amplification technology has emerged as an attractive method for detecting target DNA. Here, we describe primer generation-rolling circle amplification (PG-RCA) as an isothermal amplification method for detecting Escherichia coli O157:H7, Salmonella Typhimurium, Bacillus cereus, and Listeria monocytogenes. To improve PG-RCA sensitivity, the concentrations of the reaction components, dNTPs, phi29 DNA polymerase, and circular probes were optimized; the optimized conditions were applied to detect each target bacterium. A pair of forward and reverse circular probes that hybridized to the sense and anti-sense target genes was used in PG-RCA, exhibiting target selectivity. PG-RCA, which generated additional primers simultaneously with linear RCA and comprised multiple reaction cycles, resulted in higher accumulation of amplified DNA products than did linear RCA within the same reaction period. The threshold time (Tt) for each target gene concentration was determined based on the threshold value set in the amplification plot for PG-RCA, and a linear correlation between the Tt value and genomic DNA concentration was proven for each of the four bacteria. The PG-RCA-based assay could be applied to gene-based detection of various microorganisms and may be a useful isothermal amplification method for replacing traditional PCR methods.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"91 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141508575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

C13-apocarotenoids biosynthesis with engineered microbes 利用工程微生物进行 C13 类胡萝卜素的生物合成

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-06-27 DOI: 10.1007/s12257-024-00030-8

Jiawei Huang, Jiaying Lou, Jing Cao, Da Wu, Jiale Wang

{"title":"C13-apocarotenoids biosynthesis with engineered microbes","authors":"Jiawei Huang, Jiaying Lou, Jing Cao, Da Wu, Jiale Wang","doi":"10.1007/s12257-024-00030-8","DOIUrl":"https://doi.org/10.1007/s12257-024-00030-8","url":null,"abstract":"C13-apocarotenoids are volatile organic compounds naturally derived from the oxidative cleavage of carotenoids. These small molecules form unique aromas in flowers, fruits, and plants. They are highly valued compounds in the flavor and fragrance industry. The microbial production of C13-apocarotenoids, such as β-ionone, α-ionone, and pseudoionone, is an emerging and promising approach with inherent advantageousness of scalable output to reach the goals as stated in the United Nations Sustainable Development Goals. Many engineering efforts have been implemented continuously but very few of them proved to be successful in achieving product titer at the grams-per-liter level with the least accumulated amount of precursor carotenoids and byproducts. The efficiency of oxidative cleavage of carotenoids conducted by carotenoid cleavage dioxygenases is suggested to be the critical metabolic node to reconstruct an economically viable C13-apocarotenoids biosynthetic pathway. In this regard, we review recent advances in improving microbial biosynthesis of C13-apocarotenoids by protein and metabolic engineering. The potential strategies that could be implemented further to achieve efficient C13-apocarotenoid production are also discussed.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"29 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141508576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advancements in flavonoid production through engineering microbial systems 通过工程微生物系统生产黄酮类化合物的最新进展

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-06-27 DOI: 10.1007/s12257-024-00125-2

Yunhee Hwang, Myung Hyun Noh, Gyoo Yeol Jung

{"title":"Recent advancements in flavonoid production through engineering microbial systems","authors":"Yunhee Hwang, Myung Hyun Noh, Gyoo Yeol Jung","doi":"10.1007/s12257-024-00125-2","DOIUrl":"https://doi.org/10.1007/s12257-024-00125-2","url":null,"abstract":"Flavonoids are a class of polyphenolic compounds found in plants that offer extensive health benefits and have applications in the pharmaceutical, cosmetic, and food industries. Currently, flavonoid production largely depends on plant extraction methods, which face limitations owing to low yields and seasonal and environmental impacts. To address these issues, the potential of microbial fermentation, which leverages advances in metabolic engineering and genetic tools, has been discussed as an innovative alternative to overcome these challenges, thus offering an environmentally friendly and sustainable approach to flavonoid production. However, the integration of complex biosynthesis pathways into microbial systems presents challenges such as the inefficient expression of plant-derived genes, metabolic conflicts, and toxicity or feedback inhibition by accumulated flavonoids within the microbial cells. This comprehensive review highlights recent advancements in engineering strategies to address these challenges, focusing on biotransformation, single-strain fermentation, and co-culture systems, each with its own unique characteristics and potential for optimizing flavonoid production in a cost-effective and scalable manner.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"12 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141529698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Data synchronization techniques and their impact on the prediction performance of automated recalibrated soft sensors in bioprocesses 数据同步技术及其对生物过程中自动重新校准软传感器预测性能的影响

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-06-24 DOI: 10.1007/s12257-024-00120-7

Manuel Siegl, Dominik Geier, Björn Andreeßen, Sebastian Max, Esther Mose, Michael Zavrel, Thomas Becker

{"title":"Data synchronization techniques and their impact on the prediction performance of automated recalibrated soft sensors in bioprocesses","authors":"Manuel Siegl, Dominik Geier, Björn Andreeßen, Sebastian Max, Esther Mose, Michael Zavrel, Thomas Becker","doi":"10.1007/s12257-024-00120-7","DOIUrl":"https://doi.org/10.1007/s12257-024-00120-7","url":null,"abstract":"Innovative soft sensor concepts can recalibrate automatically when the prediction performance decreases due to variations in raw materials, biological variability, and changes in process strategies. For automatic recalibration, data sets are selected from a data pool based on distance-based similarity criteria and then used for calibration. Nevertheless, the most appropriate data sets often are not reliably selected due to variances in the location of landmarks and process length of the bioprocesses. This can be overcome by synchronization methods that align the historical data sets with the current process and increase the accuracy of automatic selection and recalibration. This study investigated two different synchronization methods (dynamic time warping and curve registration) as preprocessing for the automatic selection of data sets using a distance-based similarity criterion for soft sensor recalibration. The prediction performance of the two soft sensors without synchronization was compared to the variants with synchronization and evaluated by comparing the normalized root mean squared errors. Curve registration improved the prediction performance on average by 24% (Pichia pastoris) and 9% (Bacillus subtilis). Using dynamic time warping, no substantial improvement in prediction performance could be achieved. A major factor behind this was the loss of information due to singularities caused by the changing process characteristics. The evaluation was performed on two target variables of real bioprocesses: biomass concentration prediction in P. pastoris and product concentration prediction in B. subtilis.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"17 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141529699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pre-activation of hypoxia-inducible factor 1-α using prolyl hydroxylase domain inhibitors reduces cisplatin-induced nephrotoxicity 使用脯氨酰羟化酶结构域抑制剂预先激活缺氧诱导因子 1-α 可降低顺铂诱导的肾毒性

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-06-22 DOI: 10.1007/s12257-024-00123-4

Bomin Kim, Soonjo Kwon

{"title":"Pre-activation of hypoxia-inducible factor 1-α using prolyl hydroxylase domain inhibitors reduces cisplatin-induced nephrotoxicity","authors":"Bomin Kim, Soonjo Kwon","doi":"10.1007/s12257-024-00123-4","DOIUrl":"https://doi.org/10.1007/s12257-024-00123-4","url":null,"abstract":"Cisplatin is a widely used, highly effective chemotherapy drug that has a critical nephrotoxic side effect associated with acute kidney injury. Hypoxia pre-treatment is one of the methods used to reduce cisplatin-induced renal toxicity, but the exact cellular process associated with this protective effect is not clearly understood. Hypoxia-inducible factor 1 alpha (HIF-1α), the main transcription factor under hypoxia, may play a crucial role in this protective effect. To verify this, the degree of HIF-1α activation was investigated. Renal proximal tubular epithelial cells (HK-2) were treated with cisplatin following exposure to FG-4592 and CoCl2, prolyl hydroxylase domain (PHD) inhibitors that stabilize HIF-1α. Roxadustat (FG-4592) is a PHD inhibitor recently approved by the European medicines agency (EMA) for the treatment of anemia. Hypoxia pre-treatment with PHD inhibitors presented a protective effect against cisplatin-induced kidney injury. In addition, hypoxia pre-treatment relieved oxidative stress by hypoxia response genes sufficiently expressed under hypoxic pre-conditions. In conclusion, we investigated the correlation between the degree of HIF-1α pre-activation and the reduction in cisplatin-induced nephrotoxicity using PHD inhibitors. This study extends the applicability of PHD inhibitors as palliators of cisplatin-induced nephrotoxicity and provides valuable insights into overcoming the limitations of cisplatin use.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"35 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141529700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-free supernatant-assisted biogenic silver nanoparticles enhance the antibacterial efficacy of communicating bacterial pathogens 无细胞超物质辅助的生物银纳米粒子增强了传播细菌病原体的抗菌功效

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-06-21 DOI: 10.1007/s12257-024-00122-5

Raghavan Srimathi, Tesalonika Sondak, Kwang-sun Kim

{"title":"Cell-free supernatant-assisted biogenic silver nanoparticles enhance the antibacterial efficacy of communicating bacterial pathogens","authors":"Raghavan Srimathi, Tesalonika Sondak, Kwang-sun Kim","doi":"10.1007/s12257-024-00122-5","DOIUrl":"https://doi.org/10.1007/s12257-024-00122-5","url":null,"abstract":"The use of nanoparticles (NPs) as an alternative to the current generation of conventional antibiotics has exploded in the research community in recent years, as evidence of the superiority of NPs over antibiotics in the treatment of pathogens has been steadily presented. However, therapy with NPs may result in the removal of both multidrug-resistant (MDR) pathogens and commensal bacteria due to the broad-spectrum activity of NPs and the non-specificity of target bacteria. Therefore, the fabrication of MDR-pathogen-targeting NPs is necessary. In this study, biogenic silver nanoparticles (Bio-AgNPs) were synthesized using bacterial cell-free supernatant from three communicating gram-negative bacteria. The size, physical features, and morphology of the AgNPs were characterized by dynamic light scattering (an average size of 158–168 nm), X-ray diffraction (co-ordinate patterns), and transmission electron microscopy (spherical structure). The antibacterial activity of the Bio-AgNPs as minimum inhibitory concentration values was obtained between 0.8 and > 6.4 μg mL−1 for bacterial strains. Mechanistic studies of Bio-AgNPs have revealed that biofilm inhibition, protein leakage, hyperproduction of reactive oxygen species, and physical cell damage are plausible mechanisms underlying the activity of Bio-AgNPs against gram-negative pathogens. Overall, the Bio-AgNPs synthesized in this study may bolster the potential use of Bio-AgNPs as a stand-in for traditional antibiotics, and offer potential specificity against bacterial targets.<h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>\u0000","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"51 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141508577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of genes associated with accelerated biological ageing through computational analysis: a systematic review 通过计算分析确定与加速生物老化相关的基因：系统综述

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-06-04 DOI: 10.1007/s12257-024-00113-6

Shreya Chandrakant Desai, A. Dannie Macrin, T. Senthilvelan, Rames C. Panda

{"title":"Identification of genes associated with accelerated biological ageing through computational analysis: a systematic review","authors":"Shreya Chandrakant Desai, A. Dannie Macrin, T. Senthilvelan, Rames C. Panda","doi":"10.1007/s12257-024-00113-6","DOIUrl":"https://doi.org/10.1007/s12257-024-00113-6","url":null,"abstract":"The present review has mainly focused on a systematic investigation of the genes responsible for biological ageing. Ageing has been defined as a successive decline in biological functions, leading to age-associated disorders, which have caused death. Cell homeostasis has been disturbed due to multiple factors such as accumulation of DNA damage, decrease in telomeres, replicative senescence, cell division, metabolism, respiration, autophagy, calorie management, and genetic integrity. This imbalance in cell homeostasis has a major impact on the accelerated biological ageing process. Increased risk of age-associated disorders and mortality rates makes it necessary to know the cellular and molecular mechanisms behind it. This current study provides an overview of genes and their functions associated with dysregulation in core cellular functions such as replication, genetic stability, metabolism, respiration, and autophagy. The genes associated with these biological processes have been identified through a comprehensive literature survey and additional genes were included based on the outcome of STRING analysis. These genes were functionally enriched using gene ontology. Finally, a selected set of genes was mapped with 74 biological functions. Then, a correlation map was plotted to bring out genes with maximum impact on the biological processes involved in ageing. This study not only observed the most commonly known players such as mTOR and SIRT1 but also noticed less-reported genes such as ATM, LRRK2, ERCC1, ATG5, and BECN1 which were also found to be highly impacting the process of biological ageing. Additionally, the gerontology of these top five less-reported genes also has been explored.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"106 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141254007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0