Biotechnology and Bioprocess Engineering最新文献_第4页

Data synchronization techniques and their impact on the prediction performance of automated recalibrated soft sensors in bioprocesses 数据同步技术及其对生物过程中自动重新校准软传感器预测性能的影响

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-06-24 DOI: 10.1007/s12257-024-00120-7

Manuel Siegl, Dominik Geier, Björn Andreeßen, Sebastian Max, Esther Mose, Michael Zavrel, Thomas Becker

{"title":"Data synchronization techniques and their impact on the prediction performance of automated recalibrated soft sensors in bioprocesses","authors":"Manuel Siegl, Dominik Geier, Björn Andreeßen, Sebastian Max, Esther Mose, Michael Zavrel, Thomas Becker","doi":"10.1007/s12257-024-00120-7","DOIUrl":"https://doi.org/10.1007/s12257-024-00120-7","url":null,"abstract":"Innovative soft sensor concepts can recalibrate automatically when the prediction performance decreases due to variations in raw materials, biological variability, and changes in process strategies. For automatic recalibration, data sets are selected from a data pool based on distance-based similarity criteria and then used for calibration. Nevertheless, the most appropriate data sets often are not reliably selected due to variances in the location of landmarks and process length of the bioprocesses. This can be overcome by synchronization methods that align the historical data sets with the current process and increase the accuracy of automatic selection and recalibration. This study investigated two different synchronization methods (dynamic time warping and curve registration) as preprocessing for the automatic selection of data sets using a distance-based similarity criterion for soft sensor recalibration. The prediction performance of the two soft sensors without synchronization was compared to the variants with synchronization and evaluated by comparing the normalized root mean squared errors. Curve registration improved the prediction performance on average by 24% (Pichia pastoris) and 9% (Bacillus subtilis). Using dynamic time warping, no substantial improvement in prediction performance could be achieved. A major factor behind this was the loss of information due to singularities caused by the changing process characteristics. The evaluation was performed on two target variables of real bioprocesses: biomass concentration prediction in P. pastoris and product concentration prediction in B. subtilis.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"17 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141529699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pre-activation of hypoxia-inducible factor 1-α using prolyl hydroxylase domain inhibitors reduces cisplatin-induced nephrotoxicity 使用脯氨酰羟化酶结构域抑制剂预先激活缺氧诱导因子 1-α 可降低顺铂诱导的肾毒性

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-06-22 DOI: 10.1007/s12257-024-00123-4

Bomin Kim, Soonjo Kwon

{"title":"Pre-activation of hypoxia-inducible factor 1-α using prolyl hydroxylase domain inhibitors reduces cisplatin-induced nephrotoxicity","authors":"Bomin Kim, Soonjo Kwon","doi":"10.1007/s12257-024-00123-4","DOIUrl":"https://doi.org/10.1007/s12257-024-00123-4","url":null,"abstract":"Cisplatin is a widely used, highly effective chemotherapy drug that has a critical nephrotoxic side effect associated with acute kidney injury. Hypoxia pre-treatment is one of the methods used to reduce cisplatin-induced renal toxicity, but the exact cellular process associated with this protective effect is not clearly understood. Hypoxia-inducible factor 1 alpha (HIF-1α), the main transcription factor under hypoxia, may play a crucial role in this protective effect. To verify this, the degree of HIF-1α activation was investigated. Renal proximal tubular epithelial cells (HK-2) were treated with cisplatin following exposure to FG-4592 and CoCl2, prolyl hydroxylase domain (PHD) inhibitors that stabilize HIF-1α. Roxadustat (FG-4592) is a PHD inhibitor recently approved by the European medicines agency (EMA) for the treatment of anemia. Hypoxia pre-treatment with PHD inhibitors presented a protective effect against cisplatin-induced kidney injury. In addition, hypoxia pre-treatment relieved oxidative stress by hypoxia response genes sufficiently expressed under hypoxic pre-conditions. In conclusion, we investigated the correlation between the degree of HIF-1α pre-activation and the reduction in cisplatin-induced nephrotoxicity using PHD inhibitors. This study extends the applicability of PHD inhibitors as palliators of cisplatin-induced nephrotoxicity and provides valuable insights into overcoming the limitations of cisplatin use.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"35 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141529700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-free supernatant-assisted biogenic silver nanoparticles enhance the antibacterial efficacy of communicating bacterial pathogens 无细胞超物质辅助的生物银纳米粒子增强了传播细菌病原体的抗菌功效

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-06-21 DOI: 10.1007/s12257-024-00122-5

Raghavan Srimathi, Tesalonika Sondak, Kwang-sun Kim

{"title":"Cell-free supernatant-assisted biogenic silver nanoparticles enhance the antibacterial efficacy of communicating bacterial pathogens","authors":"Raghavan Srimathi, Tesalonika Sondak, Kwang-sun Kim","doi":"10.1007/s12257-024-00122-5","DOIUrl":"https://doi.org/10.1007/s12257-024-00122-5","url":null,"abstract":"The use of nanoparticles (NPs) as an alternative to the current generation of conventional antibiotics has exploded in the research community in recent years, as evidence of the superiority of NPs over antibiotics in the treatment of pathogens has been steadily presented. However, therapy with NPs may result in the removal of both multidrug-resistant (MDR) pathogens and commensal bacteria due to the broad-spectrum activity of NPs and the non-specificity of target bacteria. Therefore, the fabrication of MDR-pathogen-targeting NPs is necessary. In this study, biogenic silver nanoparticles (Bio-AgNPs) were synthesized using bacterial cell-free supernatant from three communicating gram-negative bacteria. The size, physical features, and morphology of the AgNPs were characterized by dynamic light scattering (an average size of 158–168 nm), X-ray diffraction (co-ordinate patterns), and transmission electron microscopy (spherical structure). The antibacterial activity of the Bio-AgNPs as minimum inhibitory concentration values was obtained between 0.8 and > 6.4 μg mL−1 for bacterial strains. Mechanistic studies of Bio-AgNPs have revealed that biofilm inhibition, protein leakage, hyperproduction of reactive oxygen species, and physical cell damage are plausible mechanisms underlying the activity of Bio-AgNPs against gram-negative pathogens. Overall, the Bio-AgNPs synthesized in this study may bolster the potential use of Bio-AgNPs as a stand-in for traditional antibiotics, and offer potential specificity against bacterial targets.<h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>\u0000","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"51 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141508577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of genes associated with accelerated biological ageing through computational analysis: a systematic review 通过计算分析确定与加速生物老化相关的基因：系统综述

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-06-04 DOI: 10.1007/s12257-024-00113-6

Shreya Chandrakant Desai, A. Dannie Macrin, T. Senthilvelan, Rames C. Panda

{"title":"Identification of genes associated with accelerated biological ageing through computational analysis: a systematic review","authors":"Shreya Chandrakant Desai, A. Dannie Macrin, T. Senthilvelan, Rames C. Panda","doi":"10.1007/s12257-024-00113-6","DOIUrl":"https://doi.org/10.1007/s12257-024-00113-6","url":null,"abstract":"The present review has mainly focused on a systematic investigation of the genes responsible for biological ageing. Ageing has been defined as a successive decline in biological functions, leading to age-associated disorders, which have caused death. Cell homeostasis has been disturbed due to multiple factors such as accumulation of DNA damage, decrease in telomeres, replicative senescence, cell division, metabolism, respiration, autophagy, calorie management, and genetic integrity. This imbalance in cell homeostasis has a major impact on the accelerated biological ageing process. Increased risk of age-associated disorders and mortality rates makes it necessary to know the cellular and molecular mechanisms behind it. This current study provides an overview of genes and their functions associated with dysregulation in core cellular functions such as replication, genetic stability, metabolism, respiration, and autophagy. The genes associated with these biological processes have been identified through a comprehensive literature survey and additional genes were included based on the outcome of STRING analysis. These genes were functionally enriched using gene ontology. Finally, a selected set of genes was mapped with 74 biological functions. Then, a correlation map was plotted to bring out genes with maximum impact on the biological processes involved in ageing. This study not only observed the most commonly known players such as mTOR and SIRT1 but also noticed less-reported genes such as ATM, LRRK2, ERCC1, ATG5, and BECN1 which were also found to be highly impacting the process of biological ageing. Additionally, the gerontology of these top five less-reported genes also has been explored.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"106 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141254007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tn5 transposase: a key tool to decrypt random transposition Tn5 转座酶：解密随机转座的关键工具

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-06-03 DOI: 10.1007/s12257-024-00118-1

Ying Xu, Huimin Zhou, Fangyuan Song, Huayou Chen, Zhong Ni

引用次数: 0

Suppressive activities of lupeol on sepsis mouse model 羽扇豆醇对败血症小鼠模型的抑制作用

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-05-27 DOI: 10.1007/s12257-024-00112-7

Sanghee Cho, Yun Jin Park, Jinhee Lee, Jong-Sup Bae

{"title":"Suppressive activities of lupeol on sepsis mouse model","authors":"Sanghee Cho, Yun Jin Park, Jinhee Lee, Jong-Sup Bae","doi":"10.1007/s12257-024-00112-7","DOIUrl":"https://doi.org/10.1007/s12257-024-00112-7","url":null,"abstract":"Sepsis is a life-threatening condition triggered by the body’s extreme response to an infection, leading to widespread inflammation, organ dysfunction, and potentially fatal complications. While lupeol, a significant phytosterol found in various herbal plants, has been considered as a potential anti-cancer agent, its anti-septic activities and underlying molecular mechanisms remain unclear. The aim of this study is to investigate the effects of lupeol on a cecal ligation and puncture (CLP)-induced septic mouse model. Animals were categorized into six groups: control, CLP-operated, CLP plus maslinic acid, and CLP plus lupeol (0.5, 1, or 2 mg/kg). The assessment included survival rate, body weight changes, inflammatory cytokines, and histological analyses. Additionally, human endothelial cells were stimulated with high mobility group box1 (HMGB1) protein and lupeol, with cell viability determined. Inflammatory markers and gene expression were evaluated through enzymelinked immunosorbent assay and Western blot analysis, respectively. After CLP surgery, the group treated with lupeol showed improved survival rates and body weight compared to the untreated control group. Lupeol treatment also decreased levels of tumor necrosis factor (TNF)-α, interleukin-1β, nitric oxide, and cytokines associated with kidney inflammation. When administered to HMGB1-activated cells, lupeol reduced the expression of Toll-like receptor 4 and TNF-α, while simultaneously activating phosphoinositide 3-kinase/AKT signaling to enhance cell survival. In conclusion, lupeol demonstrated anti-inflammatory properties and conferred protective effects against CLP-induced sepsis, reinforcing cell survival in the face of septic responses.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"8 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141166754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of in vitro lycopene biosynthesis from geranyl pyrophosphate employing cell-free protein synthesis 利用无细胞蛋白质合成技术，从焦磷酸香叶酯中开发体外番茄红素生物合成技术

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-05-25 DOI: 10.1007/s12257-024-00111-8

Young Hwan Goh, Ye Chan Kim, Sang Hun Jeong, Sangwoo Joo, You Kyoung Kwon, Hyunseok Yoon, Seohee Jung, Taresh P. Khobragade, Pritam Giri, Seonga Lim, Subin Yun, Sunga Cho, Sang Hyun Lee, Woo-Jae Chung, Jae Eun Lim, Taek Jin Kang, Hyungdon Yun

{"title":"Development of in vitro lycopene biosynthesis from geranyl pyrophosphate employing cell-free protein synthesis","authors":"Young Hwan Goh, Ye Chan Kim, Sang Hun Jeong, Sangwoo Joo, You Kyoung Kwon, Hyunseok Yoon, Seohee Jung, Taresh P. Khobragade, Pritam Giri, Seonga Lim, Subin Yun, Sunga Cho, Sang Hyun Lee, Woo-Jae Chung, Jae Eun Lim, Taek Jin Kang, Hyungdon Yun","doi":"10.1007/s12257-024-00111-8","DOIUrl":"https://doi.org/10.1007/s12257-024-00111-8","url":null,"abstract":"Lycopene is a compound classified as carotenoid, also known as tetraterpenoids, and its high antioxidative capabilities make demand in pharmaceutical and nutrient fields. For these reasons, much research on microbial production of lycopene has been developed and reported for more than two decades. Nevertheless, a standardized in vitro biosynthesis method for lycopene synthesis has not been reported to date. The major reasons of the absence of this method lie on the poor solubility of hydrophobic intermediates (geranylgeranyl pyrophosphate [GGPP] and phytoene), and the difficulty of employing membrane-binding enzyme, phytoene desaturase (CrtI) into in vitro reactions. In this study, we developed a standard method of in vitro biosynthesis of lycopene from geranyl pyrophosphate using four enzymes, namely farnesyl pyrophosphate synthase (IspA), GGPP synthase (CrtE), phytoene synthase (CrtB), phytoene desaturase (CrtI), and liposome-the key material, which can provide both hydrophobic area and a lipid membrane for the membrane-binding enzyme CrtI. Moreover, we performed a screening of the in vitro lycopene synthetic pathway using cell-free protein synthesis system, which verifies the applicability of our system as a tool for screening the lycopene synthesis pathway.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"43 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141147809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-yield β-alanine production from glucose and acetate in Escherichia coli 大肠杆菌利用葡萄糖和醋酸盐高产生产 β-丙氨酸

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-05-18 DOI: 10.1007/s12257-024-00107-4

Toan Minh Vo, Sunghoon Park

{"title":"High-yield β-alanine production from glucose and acetate in Escherichia coli","authors":"Toan Minh Vo, Sunghoon Park","doi":"10.1007/s12257-024-00107-4","DOIUrl":"https://doi.org/10.1007/s12257-024-00107-4","url":null,"abstract":"β-Alanine is a versatile amino acid with wide-range industrial applications, but its production from glucose has been limited by a low yield. This study addresses this challenge by developing efficient Escherichia coli strains with modified carbon metabolism as microbial cell factories and implementing a two-stage fermentation strategy. The introduction of aspartate decarboxylase (PanDE56S/I88M) facilitates the conversion of aspartate to β-alanine, while the overexpression of key enzymes such as phosphoenolpyruvate carboxylase and aspartate dehydrogenase increases the carbon flow from phosphoenolpyruvate to aspartate. To mitigate oxidative stress, the glutathione cycle was enhanced by overexpressing BtuE and Gor. In a bioreactor, the optimized strain achieved β-alanine production of 71.7 g/L with a yield of 1.0 mol/mol glucose, reaching a peak of 1.29 mol/mol. Notably, the utilization of acetate as a carbon feedstock enabled the production of 50 g/L of β-alanine with a 0.33 mol/mol acetate yield, showcasing the potential for sustainable production. This research offers valuable insights into improving the carbon yield in β-alanine production, which is of great importance for industrial applications.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"2012 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141063725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Y-shaped oligonucleotides: a promising platform for enhanced therapy with siRNA and CpG Oligodeoxyribonucleotides Y 型寡核苷酸：利用 siRNA 和 CpG 寡脱氧核苷酸进行强化治疗的前景广阔的平台

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-05-12 DOI: 10.1007/s12257-024-00109-2

In Seop Yoon, Hye Jeong Nam, Cheol Am Hong

{"title":"Y-shaped oligonucleotides: a promising platform for enhanced therapy with siRNA and CpG Oligodeoxyribonucleotides","authors":"In Seop Yoon, Hye Jeong Nam, Cheol Am Hong","doi":"10.1007/s12257-024-00109-2","DOIUrl":"https://doi.org/10.1007/s12257-024-00109-2","url":null,"abstract":"Nucleic acids (DNA and RNA) have been recognized as promising building blocks to fabricate a variety of well-defined two- and three-dimensional architectures through the programmable molecular self-assembly of multiple oligomeric strands. Y-shaped oligonucleotides are currently among the most widely employed nanostructures in the field of nucleic acid nanotechnology due to their unique features, including high structural stability, excellent biocompatibility, simplicity and ease of synthesis, and precisely controlled sizes. To functionalize biological activity, Y-shaped oligonucleotides can be incorporated with therapeutic genes such as small interfering RNA (siRNA) for target gene-specific silencing and CpG oligonucleotides (CpG ODN) for the activation of innate immune responses. Compared to the linear structures of siRNA and CpG ODN, Y-shaped siRNA and CpG ODN structures have demonstrated significant potential in the treatment of various diseases due to improved serum stability and intracellular uptake. Here, we review a broad spectrum of related topics, including the design, construction, and characteristics of Y-shaped oligonucleotides with a specific focus on their potential as a promising platform for enhancing the therapeutic efficacy of siRNA and CpG ODN.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"43 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140933336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reactive extraction for the separation of glyceric acid from aqueous solutions with 2-naphthaleneboronic acid and tri-octyl methyl ammonium chloride 用 2-萘硼酸和三辛基甲基氯化铵反应萃取分离水溶液中的甘油酸

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-05-10 DOI: 10.1007/s12257-024-00110-9

Long Hoang Dang Bui, Keitaro Aoki, Tomonari Tanaka, Yuji Aso

{"title":"Reactive extraction for the separation of glyceric acid from aqueous solutions with 2-naphthaleneboronic acid and tri-octyl methyl ammonium chloride","authors":"Long Hoang Dang Bui, Keitaro Aoki, Tomonari Tanaka, Yuji Aso","doi":"10.1007/s12257-024-00110-9","DOIUrl":"https://doi.org/10.1007/s12257-024-00110-9","url":null,"abstract":"Glyceric acid (GA), a carboxylic group-containing diol, is obtained from bioresources via microbial processes. In this study, we aimed to develop a reactive extraction method to separate GA from aqueous solutions using 2-naphthaleneboronic acid (2NB) and tri-octyl methyl ammonium chloride (TOMAC). Different feed molar amounts of 2NB (0–25 µmol), TOMAC (0–500 µmol), and NaOH (0–250 µmol) were used for GA (2.5 µmol) separation. A combination of 25 µmol 2NB, 100 µmol TOMAC, and 25 µmol NaOH was determined to be optimal for GA separation, providing 66.8 ± 3.2% GA yield at pH 11. GA was extracted by 2NB and TOMAC in a coordinated manner. Moreover, effects of various carboxylic acids (acetic, lactic, succinic, malic, tartaric, and citric acids) on GA separation from aqueous solutions were investigated. Interestingly, no significant effect on GA yeild (60.3 ± 1.2–65.2 ± 2.5%) was observed regardless of the type of carboxylic acid. The optimized protocol was subsequently applied to separate GA from crude GA solution prepared by incubating glycerol with the cells of the acetic acid bacterium, Acetobacter tropicalis NBRC 16470. GA separation was achieved at a comparable level (yield: 70.6 ± 4.6% and purity: 76.1 ± 4.1%) as that achieved using a GA reagent. This study demonstrated the efficiency of the repeated use of the organic phase for GA separation, with no significant changes in GA yield. Query Text=\"Please confirm if the author names are presented accurately and in the correct sequence (given name, middle name/initial, family name). Author 1 Given name: [specify authors given name] Last name [specify authors last name]. Also, kindly confirm the details in the metadata are correct.\"","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"246 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140941911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0