Ying Xu, Huimin Zhou, Fangyuan Song, Huayou Chen, Zhong Ni
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引用次数: 0
Abstract
With the development of modern molecular biotechnology and the continuous deepening of research on gene function, Tn5 transposase has received more and more attention due to its characteristics of randomness and high efficiency in transposition. It has now been widely used as an important tool in the field of molecular biology and genetic engineering. Known for its "cut-and-paste" mechanism that allows for the integration or mobilization of DNA sequences within genomes, making it good at library construction of high-throughput sequencing. However, the weakness still exists in Tn5 transposase that hinders its further application. This article primarily reviews the architecture, catalytic mechanism, and functional modification of Tn5 transposase, as well as its application in advanced transgenic editing and library construction of sequencing. Furthermore, it provides prospects for future research in this field.
期刊介绍：
Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
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