Suppressive activities of lupeol on sepsis mouse model

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Sanghee Cho, Yun Jin Park, Jinhee Lee, Jong-Sup Bae
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Abstract

Sepsis is a life-threatening condition triggered by the body’s extreme response to an infection, leading to widespread inflammation, organ dysfunction, and potentially fatal complications. While lupeol, a significant phytosterol found in various herbal plants, has been considered as a potential anti-cancer agent, its anti-septic activities and underlying molecular mechanisms remain unclear. The aim of this study is to investigate the effects of lupeol on a cecal ligation and puncture (CLP)-induced septic mouse model. Animals were categorized into six groups: control, CLP-operated, CLP plus maslinic acid, and CLP plus lupeol (0.5, 1, or 2 mg/kg). The assessment included survival rate, body weight changes, inflammatory cytokines, and histological analyses. Additionally, human endothelial cells were stimulated with high mobility group box1 (HMGB1) protein and lupeol, with cell viability determined. Inflammatory markers and gene expression were evaluated through enzymelinked immunosorbent assay and Western blot analysis, respectively. After CLP surgery, the group treated with lupeol showed improved survival rates and body weight compared to the untreated control group. Lupeol treatment also decreased levels of tumor necrosis factor (TNF)-α, interleukin-1β, nitric oxide, and cytokines associated with kidney inflammation. When administered to HMGB1-activated cells, lupeol reduced the expression of Toll-like receptor 4 and TNF-α, while simultaneously activating phosphoinositide 3-kinase/AKT signaling to enhance cell survival. In conclusion, lupeol demonstrated anti-inflammatory properties and conferred protective effects against CLP-induced sepsis, reinforcing cell survival in the face of septic responses.

Abstract Image

羽扇豆醇对败血症小鼠模型的抑制作用
败血症是一种危及生命的疾病,由机体对感染的极端反应引发,导致广泛的炎症、器官功能障碍和潜在的致命并发症。羽扇豆醇是一种存在于多种草本植物中的重要植物甾醇,被认为是一种潜在的抗癌剂,但它的抗败血症活性及其潜在的分子机制仍不清楚。本研究旨在探讨羽扇豆醇对盲肠结扎和穿刺(CLP)诱导的败血症小鼠模型的影响。动物被分为六组:对照组、CLP-操作组、CLP 加马斯林酸组、CLP 加露贝醇组(0.5、1 或 2 mg/kg)。评估包括存活率、体重变化、炎症细胞因子和组织学分析。此外,还用高迁移率基团框1(HMGB1)蛋白和羽扇豆醇刺激人内皮细胞,测定细胞活力。炎症标记物和基因表达分别通过酶联免疫吸附试验和 Western 印迹分析进行评估。与未接受治疗的对照组相比,接受羽扇豆醇治疗组的存活率和体重均有所提高。羽扇豆醇治疗还能降低肿瘤坏死因子(TNF)-α、白细胞介素-1β、一氧化氮和与肾脏炎症相关的细胞因子的水平。在给 HMGB1 激活的细胞施药时,羽扇豆醇可减少 Toll 样受体 4 和 TNF-α 的表达,同时激活磷脂酰肌醇 3- 激酶/AKT 信号,从而提高细胞存活率。总之,羽扇豆醇具有抗炎特性,对中性粒细胞白血病诱导的败血症具有保护作用,能在败血症反应中提高细胞存活率。
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来源期刊
Biotechnology and Bioprocess Engineering
Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物
CiteScore
5.00
自引率
12.50%
发文量
79
审稿时长
3 months
期刊介绍: Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
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