Biotechnology and Bioprocess Engineering最新文献_第5页

Intracellular cholesterol efflux effects of mannose-beta cyclodextrin conjugates on cholesterol-laden foam cells 甘露糖-β-环糊精共轭物对胆固醇载脂泡沫细胞的细胞内胆固醇外流作用

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-04-10 DOI: 10.1007/s12257-024-00101-w

Woojeong Lee, Yong Geun Lim, Yeong Jun Song, Kyeongsoon Park

引用次数: 0

Memristor device based on bioengineered elastin-like polypeptide and its bionanohybrid 基于生物工程弹性蛋白样多肽及其仿生杂交技术的薄膜晶体管装置

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-04-06 DOI: 10.1007/s12257-024-00102-9

Kyungmin Lee, Hunsang Jung, Heelak Choi, Jong In Won, Hyun Ho Lee

{"title":"Memristor device based on bioengineered elastin-like polypeptide and its bionanohybrid","authors":"Kyungmin Lee, Hunsang Jung, Heelak Choi, Jong In Won, Hyun Ho Lee","doi":"10.1007/s12257-024-00102-9","DOIUrl":"https://doi.org/10.1007/s12257-024-00102-9","url":null,"abstract":"In this study, bioengineered and biosynthesized elastin-like polypeptide (ELP) was adopted for a non-volatile memory resistive switching device or a memristor. The ELP mimicked from elastin of mammal was synthetically produced in polypeptide by Escherichia coli gene recombination. They were composed of a repeating pentapeptide sequence having [Val-Pro-Gly-Val-Gly]32 sequence for bioelectronic devices with ELP showing multiple electrical resistance states between a high resistance state and a low resistance state through an applied electrical field. In addition, the ELP-coated 5-nm gold nanoparticles (Au NPs) layer was also to be biomaterials used for nanobiohybrid memristive devices. Simple metal–insulator–metal device structure and lateral electrode device with ELP layer on 5-nm Au NPs could show neuromorphic adaptive current–voltage (I–V) behavior and electrical stimulus-induced potentiation and depression in a hydrogel state. In addition, with an introduction of a neurotransmitter cortisol’s specific antibody, a preliminary sensing protocol was also examined with the nanobiohybrid device. Therefore, the introduction of ELP into neuromorphic device is regarded as the cornerstone for the development of biocompatible bioelectronic devices that can be integrated into human bionics for future artificial neuromorphic format.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"10 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140574039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and optimization of a continuous purification process using ion-exchange periodic counter-current chromatography for a low-titer enzyme 利用离子交换周期性逆流色谱法设计和优化低滴度酶的连续纯化工艺

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-04-04 DOI: 10.1007/s12257-024-00099-1

Kwanyoung Ko, Min-Jung Kim, Dasom Kim, Kangyun Seo, Sangho Lee

{"title":"Design and optimization of a continuous purification process using ion-exchange periodic counter-current chromatography for a low-titer enzyme","authors":"Kwanyoung Ko, Min-Jung Kim, Dasom Kim, Kangyun Seo, Sangho Lee","doi":"10.1007/s12257-024-00099-1","DOIUrl":"https://doi.org/10.1007/s12257-024-00099-1","url":null,"abstract":"A continuous purification process can be beneficial to the purification of biologics due to its higher productivity and efficiency than a conventional batch purification process. However, regulatory issues and lack of established cases render deployment of the continuous process difficult in industrial settings. Here we report a case study for design and optimization of an advanced continuous process for purifying a low-titer enzyme as a model biologic. To convert a conventional batch process to an advanced continuous one in purification of biologics, conventional unit operations (UOs), including ultrafiltration/diafiltration (UF/DF) and batch chromatography, were replaced by advanced ones such as in-line dilution conditioning (IDC) and periodic counter-current chromatography (PCC). The UF/DF UO was changed to IDC UO to adjust pH and conductivity. The mixing ratio of the sample and the conditioning buffer in IDC was determined by experiments with three buffers. PCC was optimized with two variables, column height and sample loading residence time, as the delta pressure in the columns was less than 1.0 bar. A graph indicating the operating area was plotted to efficiently control the PCC. Although the sample volume increased in IDC, PCC had a complementary advantage in that purification was performed faster than batch chromatography. We observed at least 25% increase in economic advantage when the advanced continuous process was applied to purify a low-titer enzyme. We propose not only a continuous process with the substitution of UF/DF and batch chromatography with IDC and PCC but also a method to optimize PCC by plotting operating areas.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"4 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140574037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptides conjugation on biomaterials: chemical conjugation approaches and their promoted multifunction for biomedical applications 生物材料上的多肽共轭：化学共轭方法及其促进的生物医学应用多功能性

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-04-02 DOI: 10.1007/s12257-024-00095-5

引用次数: 0

Selective cytotoxicity of citrate-stabilized gold nanorods against aggressive cancer cells and their potential in the melanoma treatment 柠檬酸盐稳定金纳米棒对侵袭性癌细胞的选择性细胞毒性及其治疗黑色素瘤的潜力

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-04-01 DOI: 10.1007/s12257-024-00098-2

{"title":"Selective cytotoxicity of citrate-stabilized gold nanorods against aggressive cancer cells and their potential in the melanoma treatment","authors":"","doi":"10.1007/s12257-024-00098-2","DOIUrl":"https://doi.org/10.1007/s12257-024-00098-2","url":null,"abstract":"<h3>Abstract</h3> While personalized cancer therapies have improved treatment efficacy for specific cancer types, cancers with unclear genetic factors remain a challenge. Gold nanorods (GNRs) have gained attention as cancer therapies due to their anticancer effects even without light irradiation. This study investigates the anticancer effects of citrate-stabilized gold nanorods (CGNRs) on melanoma cell lines and tumors, as well as the factors influencing their efficacy. The study found that CGNRs exhibited strong sensitivity in specific cancer cells but not in normal cells. Cell viability analysis showed that CGNRs induce cell death in a concentration-dependent manner, and their anticancer effects are mainly due to necrosis. In vivo experiments using a murine melanoma model showed that intratumoral administration of CGNRs significantly suppressed tumor growth without body weight loss. Interestingly, our results suggest that the anticancer effects of CGNRs were independent of their cellular uptake efficiency but may be highly correlated with the aggressiveness of cancer cells. Overall, the results suggest that CGNRs have promising potential as a specific treatment for melanoma, especially for aggressively proliferating cells, and may represent an alternative or complementary therapy to conventional chemotherapy.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"41 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140573818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrolyzed oyster extracts suppress lipopolysaccharide-mediated inflammation and oxidative stress in RAW264.7 murine macrophages 水解牡蛎提取物可抑制 RAW264.7 小鼠巨噬细胞中脂多糖介导的炎症和氧化应激反应

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-04-01 DOI: 10.1007/s12257-024-00094-6

{"title":"Hydrolyzed oyster extracts suppress lipopolysaccharide-mediated inflammation and oxidative stress in RAW264.7 murine macrophages","authors":"","doi":"10.1007/s12257-024-00094-6","DOIUrl":"https://doi.org/10.1007/s12257-024-00094-6","url":null,"abstract":"<h3>Abstract</h3> Oysters are rich nutrition sources that contain polysaccharides, proteins, peptides, phenolic compounds, minerals, and vitamins. In the search for compounds that exert anti-inflammatory effects in macrophages, we prepared hydrolyzed oyster (Magallana gigas) extracts (HOE) and evaluated their protective effects against inflammation and oxidative stress in RAW264.7 murine macrophages treated with lipopolysaccharide (LPS). As expected, LPS notably stimulated the generation of inflammatory molecules and associated regulatory proteins. However, HOE effectively blocked these effects in a concentration-dependent manner. This suppressive effect of HOE on inflammation appears to be responsible for blocking nuclear factor-κB (NF-κB) signaling. In addition, LPS-mediated reactive oxygen species generation RAW264.7 cells was highly inhibited by HOE treatment, which appears to be partially regulated by nuclear factor erythroid 2-related factor 2 (Nrf2). Moreover, HOE at high concentrations inhibited pro-inflammatory mediators and cytokines to a greater extent than BAY7085, a pharmacological NF-κB inhibitor, in LPS-treated RAW264.7 cells. Taken together, our results show that HOE effectively inhibits inflammation and oxidative stress via modulating the NF-κB and/or Nrf2 signaling in RAW264.7 macrophages and can be a potential therapeutic agent to prevent inflammation-related diseases.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"27 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved enzyme-linked immunosorbent assay using surface-adhesive antibody-oriented immobilizing biolinker: a proof-of-concept study 使用面向抗体的表面粘附固定生物链接剂改进酶联免疫吸附测定法：概念验证研究

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-27 DOI: 10.1007/s12257-024-00093-7

Ae Sol Lee, Hye Ryoung Heo, Chang Sup Kim, Hyung Joon Cha

引用次数: 0

Oligopeptides derived from rice protein hydrolysates: absorption, bioactivity, preparation, debittering, and application 从大米蛋白水解物中提取的寡肽：吸收、生物活性、制备、去杂质和应用

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-25 DOI: 10.1007/s12257-024-00092-8

Zheng-Fei Yan, Yi-Mei Cai, Yong-Kun Xiao, Shuai Yuan, Xue-Yi Qiao, Bo Xu, Jing Wu

引用次数: 0

3D microcapsules for human bone marrow-derived mesenchymal stem cell biomanufacturing in a vertical-wheel bioreactor 用于在垂直轮式生物反应器中进行人类骨髓间充质干细胞生物制造的三维微囊

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-21 DOI: 10.1007/s12257-024-00069-7

引用次数: 0

Development of a glucose enzyme fuel cell based on thin film electrode using biocatalysts 利用生物催化剂开发基于薄膜电极的葡萄糖酶燃料电池

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-21 DOI: 10.1007/s12257-024-00065-x

Dong Sup Kim, Xiaoguang Yang, Abdus Sobhan, Chulhwan Park, Seung Wook Kim, Jinyoung Lee

{"title":"Development of a glucose enzyme fuel cell based on thin film electrode using biocatalysts","authors":"Dong Sup Kim, Xiaoguang Yang, Abdus Sobhan, Chulhwan Park, Seung Wook Kim, Jinyoung Lee","doi":"10.1007/s12257-024-00065-x","DOIUrl":"https://doi.org/10.1007/s12257-024-00065-x","url":null,"abstract":"Artificial transplantation of the human body, which requires high technology, has been an attractive issue in the 4th industrial revolution era. The artificial equipment for human applications could contain a small-scale power supply. Enzyme fuel cells (EFCs) that generate green energy are being researched for use as the power supply for pacemakers, insulin pump, and retinal implant in human body. This study focused on an (EFC) using thin film electrodes-based on enzyme immobilization technology. The performance of this EFC was improved by enzyme immobilization and electron transfer. To improve the electron transfer, the GO/Co/chitosan composite was modified on the surface of thin film electrode. The properties of this modified surface of thin film electrode were confirmed by analysis of field emission gun scanning electron microscopy, Fourier transform infrared spectroscopy, and atomic force microscopy. The performance of the designed EFC was optimized with immobilized redox enzyme on the modified electrode. The highest power density and voltage are determined as 441.48 µW/cm2 and − 0.443 V by thin film electrode, respectively. The optimum conditions of the EFC were 0.1 M D-glucose, 0.1 g/L glucose oxidase, pH 7.0, and reaction time of 4 h for both two types of thin film-electrodes.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"165 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140198069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0