Biotechnology and Bioprocess Engineering最新文献_第5页

Effect of 4-phenylbutyrate addition timing on titer of Fc-fusion protein in Chinese hamster ovary cell cultures 4-苯基丁酸盐添加时间对中国仓鼠卵巢细胞培养物中 Fc 融合蛋白滴度的影响

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-05-07 DOI: 10.1007/s12257-024-00105-6

Jungae Lim, Jin-Hyuk Lim, Ji-Hoon Lee, Su-Hwan Cheon, Guewha Lee, Z-Hun Kim, Dong-Il Kim

引用次数: 0

Efficient biodegradation of low-density polyethylene by Pseudomonas plecoglossicida SYp2123 was observed through FT-IR and FE-SEM analysis 通过 FT-IR 和 FE-SEM 分析观察褶皱假单胞菌 SYp2123 对低密度聚乙烯的高效生物降解作用

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-05-06 DOI: 10.1007/s12257-024-00108-3

Ye-Jin Kim, Yeon-Hwa Kim, Ye-Rim Shin, Su-Yeong Choi, Jeong-Ann Park, Hyun-Ouk Kim, Kwang Suk Lim, Suk-Jin Ha

{"title":"Efficient biodegradation of low-density polyethylene by Pseudomonas plecoglossicida SYp2123 was observed through FT-IR and FE-SEM analysis","authors":"Ye-Jin Kim, Yeon-Hwa Kim, Ye-Rim Shin, Su-Yeong Choi, Jeong-Ann Park, Hyun-Ouk Kim, Kwang Suk Lim, Suk-Jin Ha","doi":"10.1007/s12257-024-00108-3","DOIUrl":"https://doi.org/10.1007/s12257-024-00108-3","url":null,"abstract":"Plastics have been consistently produced for their practicality and convenience; however, unmanaged plastics often end up in the ocean and decompose into nano-plastics through photolytic decomposition and weathering, negatively affecting marine life. This can eventually affect humans via the food chain, highlighting the need for effective solutions. Microbial biodegradation has been proposed as a solution to minimize the impact of nano-plastics on the environment, and degradation byproducts can be used in microbial metabolic pathways. In this study, 57 bacterial strains were isolated and identified from a waste treatment facility. Bacterial strains with lipase activity were selected on Tween80 agar plates. Additionally, strains capable of growing on minimal salt agar plates supplemented with low-density polyethylene (LDPE) beads were selected. Incubation in a minimal salt medium with LDPE beads as the sole carbon source led to the selection of Pseudomonas plecoglossicida SYp2123, which is capable of degrading LDPE. This strain was subjected to high cell density culture, and Fourier-transform infrared spectroscopy revealed chemical changes on the surface of LDPE beads. Additionally, field-emission scanning electron microscopy confirmed substantial biodegradation of the surface. P. plecoglossicida SYp2123 was able to degrade LDPE beads. This discovery shows that P. plecoglossicida can potentially be used as an environmentally friendly approach for tackling issues associated with polyethylene waste. ","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"29 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140884948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effects of bromoindoles on Escherichia coli O157:H7 biofilms 溴化吲哚对大肠杆菌 O157:H7 生物膜的抑制作用

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-04-18 DOI: 10.1007/s12257-024-00097-3

Hyejin Jeon, Bharath Reddy Boya, Gyuwon Kim, Jin-Hyung Lee, Jintae Lee

{"title":"Inhibitory effects of bromoindoles on Escherichia coli O157:H7 biofilms","authors":"Hyejin Jeon, Bharath Reddy Boya, Gyuwon Kim, Jin-Hyung Lee, Jintae Lee","doi":"10.1007/s12257-024-00097-3","DOIUrl":"https://doi.org/10.1007/s12257-024-00097-3","url":null,"abstract":"Enterohemorrhagic Escherichia coli O157:H7 (EHEC) is responsible for outbreaks of hemorrhagic colitis worldwide, but no effective therapy exists for EHEC infections. EHEC readily forms antimicrobial-tolerant biofilms on various biotic and abiotic surfaces. Here, we investigated the antimicrobial and antibiofilm abilities of 16 halogenated (fluoro-, chloro-, bromo-, or iodo-) indoles and indole against a pathogenic EHEC strain. Antibiofilm activities followed the order chloroindoles > bromoindoles > indole > fluoroindoles. For example, the minimum inhibitory concentrations (MICs) of 4-bromoindole and 5-bromoindole were 100 and 200 μg/mL, respectively, and at 20 μg/mL, they both inhibited EHEC biofilm formation by more than 61% without affecting planktonic cell growth. However, at concentrations greater than their MICs, both showed bactericidal activity. Antibiofilm effects were confirmed by scanning electron microscopy. Both 4-bromoindole and 5-bromoindole reduced swimming and swarming motility and curli formation, which are important factors for EHEC biofilm formation. Furthermore, quantitative structure–activity relationship analysis demonstrated that halogenation of indole with chlorine, bromine, or iodine at positions C-4 or C-5 promotes antimicrobial activity but that substitution at C-7 is detrimental. The study shows that halogenated indoles, particularly bromoindoles, have potential use as antimicrobial and antibiofilm therapies against EHEC.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"28 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140617124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Damnacanthus major Siebold & Zucc callus for antioxidative and moisturizing capacities using an artificial skin alternative 使用人工皮肤替代品评估大芒萁（Damnacanthus major Siebold & Zucc）胼胝的抗氧化和保湿能力

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-04-13 DOI: 10.1007/s12257-024-00103-8

Byung Man Lee, Hyun Ju Woo, Bum Jun Jang, Jae Ahn Shin, Young Min Ham, Eun Bi Jang, Sung Chun Kim, Jeong Mi Kim, Hwa Sung Shin

{"title":"Assessment of Damnacanthus major Siebold & Zucc callus for antioxidative and moisturizing capacities using an artificial skin alternative","authors":"Byung Man Lee, Hyun Ju Woo, Bum Jun Jang, Jae Ahn Shin, Young Min Ham, Eun Bi Jang, Sung Chun Kim, Jeong Mi Kim, Hwa Sung Shin","doi":"10.1007/s12257-024-00103-8","DOIUrl":"https://doi.org/10.1007/s12257-024-00103-8","url":null,"abstract":"Plants contain many useful substances; however, their availability and uniformity are constrained. Controlled cell culture is attracting attention as a method for identifying useful substances with better quality control than conventional plant extraction techniques. Artificial skin is widely used as an alternative to animal testing to evaluate the safety and efficacy of cosmetic materials. The Damnacanthus major Siebold & Zucc is an evergreen shrub of the madder family, and the anthraquinone series substances present in its roots have antioxidant and whitening effects, implying its potential to be used in cosmetics. In this study, the antioxidant, moisturizing, and phototoxic properties of the ethanol extract of its callus were assessed using an artificial skin alternative. It showed a distinct antioxidant effect over 600 μg/mL and a moisturizing effect over 125 μg/mL. Phospho-extracellular signal-regulated kinase 1/2 and cAMP response element-binding protein were found to be upregulated in the artificial skin due to the moisturizing mechanism, and phospho-NFκB repressing factor was observed to be upregulated in human epidermal keratinocytes (HaCaT cells) due to the antioxidant mechanism. Additionally, analysis of the artificial skin revealed no phototoxicity up to 1 mg/mL. The results of this study demonstrate that the ethanol extract of Damnacanthus major Siebold & Zucc can be used as a cosmetic material.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"38 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140573821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intracellular cholesterol efflux effects of mannose-beta cyclodextrin conjugates on cholesterol-laden foam cells 甘露糖-β-环糊精共轭物对胆固醇载脂泡沫细胞的细胞内胆固醇外流作用

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-04-10 DOI: 10.1007/s12257-024-00101-w

Woojeong Lee, Yong Geun Lim, Yeong Jun Song, Kyeongsoon Park

引用次数: 0

Memristor device based on bioengineered elastin-like polypeptide and its bionanohybrid 基于生物工程弹性蛋白样多肽及其仿生杂交技术的薄膜晶体管装置

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-04-06 DOI: 10.1007/s12257-024-00102-9

Kyungmin Lee, Hunsang Jung, Heelak Choi, Jong In Won, Hyun Ho Lee

{"title":"Memristor device based on bioengineered elastin-like polypeptide and its bionanohybrid","authors":"Kyungmin Lee, Hunsang Jung, Heelak Choi, Jong In Won, Hyun Ho Lee","doi":"10.1007/s12257-024-00102-9","DOIUrl":"https://doi.org/10.1007/s12257-024-00102-9","url":null,"abstract":"In this study, bioengineered and biosynthesized elastin-like polypeptide (ELP) was adopted for a non-volatile memory resistive switching device or a memristor. The ELP mimicked from elastin of mammal was synthetically produced in polypeptide by Escherichia coli gene recombination. They were composed of a repeating pentapeptide sequence having [Val-Pro-Gly-Val-Gly]32 sequence for bioelectronic devices with ELP showing multiple electrical resistance states between a high resistance state and a low resistance state through an applied electrical field. In addition, the ELP-coated 5-nm gold nanoparticles (Au NPs) layer was also to be biomaterials used for nanobiohybrid memristive devices. Simple metal–insulator–metal device structure and lateral electrode device with ELP layer on 5-nm Au NPs could show neuromorphic adaptive current–voltage (I–V) behavior and electrical stimulus-induced potentiation and depression in a hydrogel state. In addition, with an introduction of a neurotransmitter cortisol’s specific antibody, a preliminary sensing protocol was also examined with the nanobiohybrid device. Therefore, the introduction of ELP into neuromorphic device is regarded as the cornerstone for the development of biocompatible bioelectronic devices that can be integrated into human bionics for future artificial neuromorphic format.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"10 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140574039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and optimization of a continuous purification process using ion-exchange periodic counter-current chromatography for a low-titer enzyme 利用离子交换周期性逆流色谱法设计和优化低滴度酶的连续纯化工艺

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-04-04 DOI: 10.1007/s12257-024-00099-1

Kwanyoung Ko, Min-Jung Kim, Dasom Kim, Kangyun Seo, Sangho Lee

{"title":"Design and optimization of a continuous purification process using ion-exchange periodic counter-current chromatography for a low-titer enzyme","authors":"Kwanyoung Ko, Min-Jung Kim, Dasom Kim, Kangyun Seo, Sangho Lee","doi":"10.1007/s12257-024-00099-1","DOIUrl":"https://doi.org/10.1007/s12257-024-00099-1","url":null,"abstract":"A continuous purification process can be beneficial to the purification of biologics due to its higher productivity and efficiency than a conventional batch purification process. However, regulatory issues and lack of established cases render deployment of the continuous process difficult in industrial settings. Here we report a case study for design and optimization of an advanced continuous process for purifying a low-titer enzyme as a model biologic. To convert a conventional batch process to an advanced continuous one in purification of biologics, conventional unit operations (UOs), including ultrafiltration/diafiltration (UF/DF) and batch chromatography, were replaced by advanced ones such as in-line dilution conditioning (IDC) and periodic counter-current chromatography (PCC). The UF/DF UO was changed to IDC UO to adjust pH and conductivity. The mixing ratio of the sample and the conditioning buffer in IDC was determined by experiments with three buffers. PCC was optimized with two variables, column height and sample loading residence time, as the delta pressure in the columns was less than 1.0 bar. A graph indicating the operating area was plotted to efficiently control the PCC. Although the sample volume increased in IDC, PCC had a complementary advantage in that purification was performed faster than batch chromatography. We observed at least 25% increase in economic advantage when the advanced continuous process was applied to purify a low-titer enzyme. We propose not only a continuous process with the substitution of UF/DF and batch chromatography with IDC and PCC but also a method to optimize PCC by plotting operating areas.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"4 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140574037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptides conjugation on biomaterials: chemical conjugation approaches and their promoted multifunction for biomedical applications 生物材料上的多肽共轭：化学共轭方法及其促进的生物医学应用多功能性

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-04-02 DOI: 10.1007/s12257-024-00095-5

引用次数: 0

Selective cytotoxicity of citrate-stabilized gold nanorods against aggressive cancer cells and their potential in the melanoma treatment 柠檬酸盐稳定金纳米棒对侵袭性癌细胞的选择性细胞毒性及其治疗黑色素瘤的潜力

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-04-01 DOI: 10.1007/s12257-024-00098-2

{"title":"Selective cytotoxicity of citrate-stabilized gold nanorods against aggressive cancer cells and their potential in the melanoma treatment","authors":"","doi":"10.1007/s12257-024-00098-2","DOIUrl":"https://doi.org/10.1007/s12257-024-00098-2","url":null,"abstract":"<h3>Abstract</h3> While personalized cancer therapies have improved treatment efficacy for specific cancer types, cancers with unclear genetic factors remain a challenge. Gold nanorods (GNRs) have gained attention as cancer therapies due to their anticancer effects even without light irradiation. This study investigates the anticancer effects of citrate-stabilized gold nanorods (CGNRs) on melanoma cell lines and tumors, as well as the factors influencing their efficacy. The study found that CGNRs exhibited strong sensitivity in specific cancer cells but not in normal cells. Cell viability analysis showed that CGNRs induce cell death in a concentration-dependent manner, and their anticancer effects are mainly due to necrosis. In vivo experiments using a murine melanoma model showed that intratumoral administration of CGNRs significantly suppressed tumor growth without body weight loss. Interestingly, our results suggest that the anticancer effects of CGNRs were independent of their cellular uptake efficiency but may be highly correlated with the aggressiveness of cancer cells. Overall, the results suggest that CGNRs have promising potential as a specific treatment for melanoma, especially for aggressively proliferating cells, and may represent an alternative or complementary therapy to conventional chemotherapy.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"41 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140573818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrolyzed oyster extracts suppress lipopolysaccharide-mediated inflammation and oxidative stress in RAW264.7 murine macrophages 水解牡蛎提取物可抑制 RAW264.7 小鼠巨噬细胞中脂多糖介导的炎症和氧化应激反应

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-04-01 DOI: 10.1007/s12257-024-00094-6

{"title":"Hydrolyzed oyster extracts suppress lipopolysaccharide-mediated inflammation and oxidative stress in RAW264.7 murine macrophages","authors":"","doi":"10.1007/s12257-024-00094-6","DOIUrl":"https://doi.org/10.1007/s12257-024-00094-6","url":null,"abstract":"<h3>Abstract</h3> Oysters are rich nutrition sources that contain polysaccharides, proteins, peptides, phenolic compounds, minerals, and vitamins. In the search for compounds that exert anti-inflammatory effects in macrophages, we prepared hydrolyzed oyster (Magallana gigas) extracts (HOE) and evaluated their protective effects against inflammation and oxidative stress in RAW264.7 murine macrophages treated with lipopolysaccharide (LPS). As expected, LPS notably stimulated the generation of inflammatory molecules and associated regulatory proteins. However, HOE effectively blocked these effects in a concentration-dependent manner. This suppressive effect of HOE on inflammation appears to be responsible for blocking nuclear factor-κB (NF-κB) signaling. In addition, LPS-mediated reactive oxygen species generation RAW264.7 cells was highly inhibited by HOE treatment, which appears to be partially regulated by nuclear factor erythroid 2-related factor 2 (Nrf2). Moreover, HOE at high concentrations inhibited pro-inflammatory mediators and cytokines to a greater extent than BAY7085, a pharmacological NF-κB inhibitor, in LPS-treated RAW264.7 cells. Taken together, our results show that HOE effectively inhibits inflammation and oxidative stress via modulating the NF-κB and/or Nrf2 signaling in RAW264.7 macrophages and can be a potential therapeutic agent to prevent inflammation-related diseases.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"27 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0