水解牡蛎提取物可抑制 RAW264.7 小鼠巨噬细胞中脂多糖介导的炎症和氧化应激反应

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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引用次数: 0

摘要

摘要 牡蛎营养丰富,含有多糖、蛋白质、肽、酚类化合物、矿物质和维生素。为了寻找能在巨噬细胞中发挥抗炎作用的化合物,我们制备了水解牡蛎(Magallana gigas)提取物(HOE),并评估了它们在经脂多糖(LPS)处理的 RAW264.7 小鼠巨噬细胞中对炎症和氧化应激的保护作用。不出所料,LPS 显著刺激了炎症分子和相关调节蛋白的生成。然而,HOE 以浓度依赖的方式有效阻止了这些效应。HOE 对炎症的这种抑制作用似乎是由于阻断了核因子-κB(NF-κB)信号传导。此外,HOE 处理高度抑制了 LPS 介导的 RAW264.7 细胞活性氧生成,这似乎部分受核因子红细胞 2 相关因子 2(Nrf2)的调节。此外,在 LPS 处理的 RAW264.7 细胞中,高浓度 HOE 比药理 NF-κB 抑制剂 BAY7085 更能抑制促炎介质和细胞因子。综上所述,我们的研究结果表明,HOE 可通过调节 RAW264.7 巨噬细胞中的 NF-κB 和/或 Nrf2 信号转导,有效抑制炎症和氧化应激,是预防炎症相关疾病的潜在治疗药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hydrolyzed oyster extracts suppress lipopolysaccharide-mediated inflammation and oxidative stress in RAW264.7 murine macrophages

Abstract

Oysters are rich nutrition sources that contain polysaccharides, proteins, peptides, phenolic compounds, minerals, and vitamins. In the search for compounds that exert anti-inflammatory effects in macrophages, we prepared hydrolyzed oyster (Magallana gigas) extracts (HOE) and evaluated their protective effects against inflammation and oxidative stress in RAW264.7 murine macrophages treated with lipopolysaccharide (LPS). As expected, LPS notably stimulated the generation of inflammatory molecules and associated regulatory proteins. However, HOE effectively blocked these effects in a concentration-dependent manner. This suppressive effect of HOE on inflammation appears to be responsible for blocking nuclear factor-κB (NF-κB) signaling. In addition, LPS-mediated reactive oxygen species generation RAW264.7 cells was highly inhibited by HOE treatment, which appears to be partially regulated by nuclear factor erythroid 2-related factor 2 (Nrf2). Moreover, HOE at high concentrations inhibited pro-inflammatory mediators and cytokines to a greater extent than BAY7085, a pharmacological NF-κB inhibitor, in LPS-treated RAW264.7 cells. Taken together, our results show that HOE effectively inhibits inflammation and oxidative stress via modulating the NF-κB and/or Nrf2 signaling in RAW264.7 macrophages and can be a potential therapeutic agent to prevent inflammation-related diseases.

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来源期刊
Biotechnology and Bioprocess Engineering
Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物
CiteScore
5.00
自引率
12.50%
发文量
79
审稿时长
3 months
期刊介绍: Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
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