Biotechnology and Bioprocess Engineering最新文献_第7页

In silico screening and validation of different dehydrogenases to produce 2,3-butanediol in Bacillus subtilis 在枯草芽孢杆菌中生产 2,3-丁二醇的不同脱氢酶的硅学筛选和验证

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-13 DOI: 10.1007/s12257-024-00053-1

Sailee Sanjay Asolkar, M. Anju, Ravindra Kumar, Apoorva Deshmukh, Anand Ghosalkar, Pramod Kumbhar

{"title":"In silico screening and validation of different dehydrogenases to produce 2,3-butanediol in Bacillus subtilis","authors":"Sailee Sanjay Asolkar, M. Anju, Ravindra Kumar, Apoorva Deshmukh, Anand Ghosalkar, Pramod Kumbhar","doi":"10.1007/s12257-024-00053-1","DOIUrl":"https://doi.org/10.1007/s12257-024-00053-1","url":null,"abstract":"Bacillus subtilis is a natural producer of 2,3-butanediol (2,3-BDO) and has acquired “Generally Regarded as Safe\" status. It is reported to produce 2,3-BDO from synthetic sugars as well as complex and economic sugar sources such as molasses. However, the rate-limiting step in the formation of 2,3-BDO is its conversion from acetoin to 2,3-BDO by the enzyme butanediol dehydrogenase (2,3-BDH). Such 2,3-BDHs were screened based on higher affinity (lower Km) towards acetoin as substrate. The in silico docking studies were conducted for further validation, and they showed a high interaction profile for the PpBDH protein towards acetoin. Heterologous expression of these genes was studied in engineered Bacillus subtilis (BS1A1). In this study, it was seen that 2,3-BDH from Paenibacillus polymyxa ZJ-9 was reported to have higher enzyme activity levels, and in the fermentation studies, it was seen that the ratio of 2,3-BDO to acetoin was increased by 80.25%. The insights encourage further bioprocess optimization for increasing the fermentative production of 2,3-BDO. Our results provide a potential strategy to avoid the back conversion of 2,3-BDO to acetoin in an engineered Bacillus system.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"103 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140128697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient production of 1,3-propanediol from glycerol by a newly isolated soil bacterium using fed-batch fermentation 一种新分离的土壤细菌利用饲料批量发酵法从甘油中高效生产 1,3-丙二醇

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-12 DOI: 10.1007/s12257-024-00031-7

Selim Ashoor, Zhuang Yao, Chan Woo Song, Haeng Lim Lee, Hyeon Jeong Seong, Sampathkumar Palaniswamy, Jong Myoung Park, Hyohak Song, Yu-Sin Jang

引用次数: 0

Engineering potyvirus-like particles to display multiple copies of tuberculosis antigens 设计出显示多份结核病抗原的壶状病毒颗粒

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-11 DOI: 10.1007/s12257-024-00089-3

R. Princess, M. L. Stephen Raj

{"title":"Engineering potyvirus-like particles to display multiple copies of tuberculosis antigens","authors":"R. Princess, M. L. Stephen Raj","doi":"10.1007/s12257-024-00089-3","DOIUrl":"https://doi.org/10.1007/s12257-024-00089-3","url":null,"abstract":"Elicitation of antibody and cell-mediated immune responses are crucial for successful vaccine development against tuberculosis (TB). Mycobacterium tuberculosis (Mtb) antigens CFP10 and ESAT6, potent and proven vaccine candidates require appropriate adjuvants to trigger better immune response. Virus-like particles carrying repetitive copies of foreign antigens can induce both T and B cell-mediated immunity required for conferring protection against intracellular pathogens. In this study, we developed hybrid potyvirus-like particles (PVLPs) displaying mycobacterial antigens on their surface by translationally fusing the coat protein (CP) gene derived from Johnson grass mosaic virus with CFP 10 or/and ESAT 6 gene(s). The recombinant plasmids carrying fusion constructs were transformed into Escherichia coli, the fusion proteins, viz. ESAT6-CP, CP-CFP10 and ESAT6-CP-CFP10, were expressed and purified using Ni-NTA2+ affinity chromatography under denaturing conditions. The chimeric CP fusion proteins were self-assembled in vitro into PVLPs by the gradual removal of denaturing conditions. The purified hybrid PVLPs carrying Mtb antigens when injected into mice showed enhanced immunogenicity for both ESAT6 and CFP10 antigens compared to the same antigens immunized without any adjuvant. In vitro stimulation of splenocytes derived from mice immunized with chimeric PVLPs upregulates the expression of cytokines involved in TB immune response.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"366 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140128767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioanalysis of ursodeoxycholic acid and its metabolites and improved oral bioavailability using mixed micelles with poloxamer 407 and polysorbate 80 熊去氧胆酸及其代谢物的生物分析以及使用聚氧乙烯酰胺 407 和聚山梨醇酯 80 混合胶束提高口服生物利用度

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-11 DOI: 10.1007/s12257-024-00064-y

So Yeon Jeon, Chul Haeng Lee, Jihoon Lee, Ji-Hyeon Jeon, Yelim Jin, Im-Sook Song, Min-Koo Choi

{"title":"Bioanalysis of ursodeoxycholic acid and its metabolites and improved oral bioavailability using mixed micelles with poloxamer 407 and polysorbate 80","authors":"So Yeon Jeon, Chul Haeng Lee, Jihoon Lee, Ji-Hyeon Jeon, Yelim Jin, Im-Sook Song, Min-Koo Choi","doi":"10.1007/s12257-024-00064-y","DOIUrl":"https://doi.org/10.1007/s12257-024-00064-y","url":null,"abstract":"The development of analytical methods for endogenous therapeutic substances is a critical but challenging issue as obtaining a blank matrix without endogenous substance is impossible. To address this issue, we prepared a surrogate biological matrix by removing endogenous bile acids from rat plasma using a charcoal-stripped method and developed an analytical method for ursodeoxycholic acid (UDCA) and its conjugated metabolites, tauroursodeoxycholic (TUDCA) and glycoursodeoxycholic acid (GUDCA), including the use of surrogate matrices and protein precipitation method. In addition, we applied the bioanalytical method to investigate the bioavailability of UDCA-mixed micelle powder formulation (UDCA-MM). The oral bioavailability of UDCA in rats was calculated as 15.2% and increased 3.32-fold following the oral administration of UDCA-MM with the increased production of TUDCA without significant change in GUDCA. The UDCA-MM powder was prepared by thin-layer hydration and subsequent freeze-drying method in a ratio of UDCA/polysorbate 80/poloxamer 407 = 1:1:10 (w/w/w). The UDCA-MM was easily dispersed with a particle size of 16.5 ± 2.2 nm and solubility of 1120 ± 38 μg/mL, which represented a 175.3-fold increase in its solubility of UDCA. In conclusion, we developed and validated a simple and reliable bioanalytical method for UDCA, TUDCA, and GUDCA using the charcoal-stripped plasma as surrogate matrices. Our bioanalytical method successfully supported the assessment of the pharmacokinetics or bioavailability of UDCA, TUDCA, and GUDCA after the intravenous or oral dosing of UDCA and UDCA-MM. The UDCA-MM using poloxamer 407 and polysorbate 80 is a promising technique for increasing the solubility and oral absorption of UDCA.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140097260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reinforced levan-based electrospun nanofibers for application as adhesive scaffolds for tissue engineering 应用于组织工程粘合支架的增强型莱万基电纺纳米纤维

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-09 DOI: 10.1007/s12257-024-00032-6

Young Hoon Song, Jeong Hyun Seo

{"title":"Reinforced levan-based electrospun nanofibers for application as adhesive scaffolds for tissue engineering","authors":"Young Hoon Song, Jeong Hyun Seo","doi":"10.1007/s12257-024-00032-6","DOIUrl":"https://doi.org/10.1007/s12257-024-00032-6","url":null,"abstract":"The fabrication of scaffolds that mimic the structure of extracellular matrix molecules using electrospinning has great potential for various tissue engineering applications because it is advantageous for cell ingrowth and tissue formation. In particular, in the case of non-adhesive scaffolds, tissue adhesives (e.g., fibrin, glutaraldehyde) that provide strong adhesion to tissue surfaces or cells are sometimes used to efficiently utilize the desired function. Therefore, scaffolds that combine the advantages of adhesive materials while maintaining cell growth and functionality could further expand tissue engineering applications. In the present study, we developed nanofibers with greatly improved adhesive ability by blended cellulose acetate and levan, a bioadhesive polymer. Nanofibers manufactured through electrospinning exhibited stable mechanical properties through citric acid cross-linking and exhibited excellent adhesive performance with an adhesion strength of up to 3.67 MPa. In future research, we aim to expand the utility of bioadhesive nanofibers by loading nanofiber scaffolds with useful substances for diagnostic and therapeutic purposes.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"40 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140097122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial production of dextran using pineapple waste extract: a two-step statistical optimization of submerged fermentation conditions and structural characterization 利用菠萝废提取物的微生物生产葡聚糖：浸没式发酵条件和结构特征的两步统计优化

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-03-04 DOI: 10.1007/s12257-024-00002-y

Ashutosh Tripathy, Mukesh Kumar Patel, Snehasis Chakraborty

{"title":"Microbial production of dextran using pineapple waste extract: a two-step statistical optimization of submerged fermentation conditions and structural characterization","authors":"Ashutosh Tripathy, Mukesh Kumar Patel, Snehasis Chakraborty","doi":"10.1007/s12257-024-00002-y","DOIUrl":"https://doi.org/10.1007/s12257-024-00002-y","url":null,"abstract":"A two-step optimization protocol was attempted to optimize the condition for dextran production from pineapple waste using Leuconostoc mesenteroides NCIM-2198. Response surface methodology, in combination with numerical optimization technique, was explored for this purpose. In the first step, the initial medium pH (6.5–7.5), incubation temperature (25–35 °C), and time (12–60 h) were optimized from 45 full factorial runs. The maximum dextran yield was estimated as 1.46 g·[100 mL]−1 while incubated at 29.2 °C for 57.2 h at an initial pH of 7.15. Further, sucrose concentration (2–10 g·[100 mL]−1) and culture volume (3–7 mL·[100 mL]−1) were optimized from 15 experimental runs. The maximum dextran yield (1.47 g·[100 mL]−1) was obtained at 7.6 g·[100 mL]−1 of sucrose with 3 mL·[100 mL]−1 of culture volume at the previously optimized fermented broth. The response surface models were validated to explain the interaction between factors affecting dextran yield. The structural characteristics of the exopolysaccharide were analyzed. Fourier-transform infrared spectra showed that the exopolysaccharide contains similar spectral peaks as that of standard dextran. Nuclear magnetic resonance spectroscopy confirms the exopolysaccharide was dextran with mainly α-1-6 glycosidic bonds. Scanning electron microscopy explained its porous structure, which would be useful in retaining water and thus giving texturizing and viscosifying properties.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"129 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140025929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catechol- and thiol-containing binder that aggregates granular xenografts in reconstructed bone defects by mimicking mussel wet adhesion 含儿茶酚和硫醇的粘合剂可通过模拟贻贝湿粘附作用将颗粒状异种移植物聚集在重建的骨缺损中

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-22 DOI: 10.1007/s12257-024-00025-5

{"title":"Catechol- and thiol-containing binder that aggregates granular xenografts in reconstructed bone defects by mimicking mussel wet adhesion","authors":"","doi":"10.1007/s12257-024-00025-5","DOIUrl":"https://doi.org/10.1007/s12257-024-00025-5","url":null,"abstract":"<h3>Abstract</h3> Xenograft bone granules are bone graft materials that are widely used to reconstruct bone tissue in clinical practice, and their clinical success requires aggregation with a biocompatible binder. While the catechol mussel bioadhesive has been used as a key universal biomedical glue moiety, the thiols in mussel adhesive proteins have recently been shown to play key roles in mussel wet adhesion. Here, pentaerythritol tris(3-mercaptopropionate)-di-catechol (PETMP-di-catechol), a catechol- and thiol-containing binder molecule that mimics mussel adhesive proteins, was used to aggregate xenograft bone granules and reconstruct bone tissue. The PETMP-di-catechol-aggregated xenograft bone granules were most resistive to compression stress when both the catechol and thiol moieties in PETMP-di-catechol were chemically oxidized with sodium periodate. The xenograft bone granules aggregated with the oxidized PETMP-di-catechol exhibited enhanced osteogenic cellular behavior in vitro and in vivo in a rat calvarial defect model, compared to the control group treated with catechol-conjugated chitosan, a catechol-containing polymer binder devoid of thiol moieties. Hence, the thiol-containing catechol-functionalized binder exhibits improved bone tissue reconstruction properties by mimicking mussel wet adhesion. In addition, our study suggests that PETMP-di-catechol is a potential biocompatible bone binder for use in clinical settings.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"21 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139948066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted siRNA delivery to lung epithelia reduces airway inflammation in a mouse model of allergic asthma 在过敏性哮喘小鼠模型中，靶向 siRNA 运送到肺上皮细胞可减少气道炎症

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-21 DOI: 10.1007/s12257-024-00027-3

Irfan Ullah, Hyo Sung Choi, Changseon Choi, Kunho Chung, Jae Wook Jung, Gyeongju Yun, Seoyoun Heo, Yujong Yi, Eunhwa Kang, Sang-Heon Kim, Ho Joo Yoon, Taiyoun Rhim, Sang-Kyung Lee

{"title":"Targeted siRNA delivery to lung epithelia reduces airway inflammation in a mouse model of allergic asthma","authors":"Irfan Ullah, Hyo Sung Choi, Changseon Choi, Kunho Chung, Jae Wook Jung, Gyeongju Yun, Seoyoun Heo, Yujong Yi, Eunhwa Kang, Sang-Heon Kim, Ho Joo Yoon, Taiyoun Rhim, Sang-Kyung Lee","doi":"10.1007/s12257-024-00027-3","DOIUrl":"https://doi.org/10.1007/s12257-024-00027-3","url":null,"abstract":"Asthma is a chronic inflammatory disease triggered by allergic reactions in the bronchia. These reactions lead to swelling of mucous membranes, hypersecretion of mucus, and bronchoconstriction, resulting in a restricted opening of the lung airway. Allergic pulmonary inflammation and airway hyperresponsiveness are induced when Th2 cytokines, such as interleukin (IL)-4 and IL-13, bind to their cognate receptors on lung epithelial cells. Specifically, IL-13 stimulates inflammation through a multi-subunit receptor, mainly the alpha chain of the IL-4 receptor (IL-4Rα), which also plays a role in IL-4 signaling. In this study, we employed a lung epithelial cell-targeting siRNA carrier composed of a rabies virus glycoprotein-derived small peptide coupled with cationic nona-arginine and trileucine before cysteine peptide (RVG9R3LC). This carrier was complexed with siRNA, enabling targeted delivery of therapeutic siRNA to IL-4Rα (siIL4Rα) expressed in lung epithelial cells within an asthma model in vivo. Our approach demonstrated efficient gene knockdown in cultured lung epithelial cells and in vivo. Furthermore, two administrations of therapeutic siIL4Rα protected the ovalbumin-sensitized and challenged asthma mouse model from airway inflammation and excessive mucus secretion. Our findings suggest that the peptide-siRNA carrier system presents a promising therapeutic approach for respiratory inflammation.<h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"12 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139924172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing transduction efficiency of adeno-associated virus 9 by cell line engineering: implication for gene therapy potency assay 通过细胞系工程提高腺相关病毒 9 的转导效率：对基因疗法效力测定的影响

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-19 DOI: 10.1007/s12257-024-00003-x

{"title":"Enhancing transduction efficiency of adeno-associated virus 9 by cell line engineering: implication for gene therapy potency assay","authors":"","doi":"10.1007/s12257-024-00003-x","DOIUrl":"https://doi.org/10.1007/s12257-024-00003-x","url":null,"abstract":"<h3>Abstract</h3> Adeno-associated virus (AAV)-mediated gene therapy holds significant promises to treat or potentially cure various human diseases. Although AAV holds promise for their significant therapeutic potential, batch-to-batch differences can exist from manufacturing; and therefore, a potency assay is required for clinical development of AAV. Among different serotypes, due to its ability to cross blood–brain barrier and wide-spread transduction capability in vivo upon systemic administration, AAV9 has been widely utilized for the development of treatment of neurological disorders. However, as AAV9 is known to show poor transduction in vitro, establishing a robust in vitro potency assay have been difficult. To this end, we engineered HEK293T and Schwann-like cell lines to express previously identified common AAV receptor, AAVR or endogenous host factor involved in AAV endosomal escapes, GPR108 that can increase infectivity of AAVs in an attempt to increase transduction capability of AAV9. We found that AAVR overexpressed Schwann-like cell line showed significant increase in AAV9 transduction; whereas, GPR108 overexpression showed no effect on AAV9 transduction. On the other hand, GPR108 engineered HEK293T showed increase in AAV9 transduction; whereas, AAVR overexpressed HEK293T cell line showed modest increase in AAV9 transduction. Gene expression analysis showed that AAVR is highly expressed in HEK293T compared to Schwann-like cell line; whereas, GPR108 is highly expressed in Schwann-like cell line when compared to HEK293T. These results indicate that different cell lines may require different gene engineering to increase AAV9 infectivity and analysis of endogenous expression of AAV entry factors for cell line to be engineered can improve efficiency of cell line engineering for AAV transduction.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"36 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139924212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vibrio species as next-generation chassis for accelerated synthetic biology 弧菌作为加速合成生物学的下一代底盘

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-19 DOI: 10.1007/s12257-024-00023-7

Changhwan Hong, Yoojin Kim, Hyunjin Lee, Saebom Yun, Hyun Gyu Lim, Jina Yang, Sungho Jang

{"title":"Vibrio species as next-generation chassis for accelerated synthetic biology","authors":"Changhwan Hong, Yoojin Kim, Hyunjin Lee, Saebom Yun, Hyun Gyu Lim, Jina Yang, Sungho Jang","doi":"10.1007/s12257-024-00023-7","DOIUrl":"https://doi.org/10.1007/s12257-024-00023-7","url":null,"abstract":"Synthetic biology aims to establish engineering principles for biological systems by leveraging the design-build-test-learn (DBTL) cycle. Central to the success of the DBTL cycle is the selection of a suitable chassis, which is the environment in which biological designs are tested. Every step of this cycle is strongly influenced by the properties of chassis. A successful chassis must meet various criteria, prompting ongoing research regarding new candidates. Recently, species within the Vibrio genus, notably Vibrio natriegens and related strains, have emerged as promising next-generation chassis due to their rapid growth rates, versatile substrate utilization, and biosafety level 1 classification. These properties make them highly attractive for accelerating the DBTL cycle with the potential for efficient protein and metabolite production. This review emphasizes the foundational requirements for efficient engineering in synthetic biology, including genetic parts, vectors, and genome engineering technologies tailored to Vibrio species. Practical applications, such as metabolic engineering and protein expression, have been discussed, offering a comprehensive summary of recent advances. This paper also outlines the future directions and suggestions for fully unlocking the potential of Vibrio species as next-generation chassis.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"8 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139924213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0