Microfluidics-driven high-throughput phenotyping and screening in synthetic biology: from single cells to cell-free systems

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Taeok Kim, Minji Ko, Eugene Rha, Haseong Kim, Hyewon Lee
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引用次数: 0

Abstract

The interdisciplinary nature of synthetic biology merges engineering principles with biology and provides innovative solutions for issues in the biomanufacturing industry. To develop industrially applicable biocatalysts and/or microbial cell factories, a design-build-test-learn cycle-based iterative process is necessary, which is often time-consuming and labor-intensive. The integration of microfluidic technologies into synthetic biology can accelerate these processes, particularly for achieving high-throughput phenotyping and screening. In this review, we examine the potential of microfluidic technologies to revolutionize synthetic biology. Although commercial microfluidics demonstrate superior throughput for single-cell assays, their application can be limited, for example, in cases where products are retained inside the cells. Droplet microfluidics, on the other hand, is a rather flexible platform and shows high diversity in single-cell, cell-to-cell interaction-based, and cell-free reaction-based analyses. By examining previous studies, we have summarized the potential of microfluidic technologies to foster sustainable biomanufacturing and advanced biological engineering.

Abstract Image

合成生物学中微流体驱动的高通量表型和筛选:从单细胞到无细胞系统
合成生物学的跨学科性质将工程学原理与生物学融为一体,为生物制造行业的问题提供了创新解决方案。要开发工业上适用的生物催化剂和/或微生物细胞工厂,必须采用基于设计-构建-测试-学习循环的迭代流程,而这一流程往往耗时耗力。将微流控技术整合到合成生物学中可以加速这些过程,特别是实现高通量表型和筛选。在这篇综述中,我们将探讨微流控技术彻底改变合成生物学的潜力。尽管商用微流控技术在单细胞检测方面表现出了卓越的吞吐量,但其应用可能会受到限制,例如在产品保留在细胞内的情况下。另一方面,液滴微流控技术是一种相当灵活的平台,在基于单细胞、细胞间相互作用和无细胞反应的分析中表现出高度的多样性。通过研究以往的研究,我们总结了微流控技术在促进可持续生物制造和先进生物工程方面的潜力。
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来源期刊
Biotechnology and Bioprocess Engineering
Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物
CiteScore
5.00
自引率
12.50%
发文量
79
审稿时长
3 months
期刊介绍: Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
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