Biotechnology and Bioprocess Engineering最新文献_第8页

Development and optimization of a modular two-fragment LacI switch for enhanced biosensor applications 开发和优化用于增强生物传感器应用的模块化双片段 LacI 开关

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-19 DOI: 10.1007/s12257-024-00020-w

引用次数: 0

A comparison of metabolic engineering strategies applied in Yarrowia lipolytica for β-carotene production 比较应用于脂肪溶解亚罗酵母生产β-胡萝卜素的代谢工程策略

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-18 DOI: 10.1007/s12257-024-00006-8

Redife Aslıhan Uçar, Furkan Demirgül, Ömer Şimşek, Hüseyin Erten

{"title":"A comparison of metabolic engineering strategies applied in Yarrowia lipolytica for β-carotene production","authors":"Redife Aslıhan Uçar, Furkan Demirgül, Ömer Şimşek, Hüseyin Erten","doi":"10.1007/s12257-024-00006-8","DOIUrl":"https://doi.org/10.1007/s12257-024-00006-8","url":null,"abstract":"With a better understanding of the health benefits of β-carotene, the precursor of vitamin A, as well as its coloring property, the need for this carotenoid has increased in various sectors. In order to meet the increasing demand efficiently, cheaply, and sustainably, interest in heterologous β-carotene production through metabolic engineering strategies has increased in recent years. In this context, although it is not a native producer of β-carotene, Yarrowia lipolytica yeast stands out due to its metabolic, physiological, and genomic properties. Successful results have been obtained by using a series of engineering strategies, including biosynthesis pathway engineering, morphological engineering, and fermentation engineering strategies, in the production of heterologous β-carotene from Y. lipolytica. However, these strategies have various strengths and weaknesses against each other, and there are also some points open to improvement. In this review, the engineering strategies that have been applied and have the potential to be applied for the production of β-carotene from Y. lipolytica have been examined in depth, including their advantages and disadvantages, and compared with each other. Moreover, a future perspective has been presented to increase the potential of using Y. lipolytica yeast as a cell factory in β-carotene production.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"1 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139924192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidics-driven high-throughput phenotyping and screening in synthetic biology: from single cells to cell-free systems 合成生物学中微流体驱动的高通量表型和筛选：从单细胞到无细胞系统

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-16 DOI: 10.1007/s12257-024-00016-6

Taeok Kim, Minji Ko, Eugene Rha, Haseong Kim, Hyewon Lee

{"title":"Microfluidics-driven high-throughput phenotyping and screening in synthetic biology: from single cells to cell-free systems","authors":"Taeok Kim, Minji Ko, Eugene Rha, Haseong Kim, Hyewon Lee","doi":"10.1007/s12257-024-00016-6","DOIUrl":"https://doi.org/10.1007/s12257-024-00016-6","url":null,"abstract":"The interdisciplinary nature of synthetic biology merges engineering principles with biology and provides innovative solutions for issues in the biomanufacturing industry. To develop industrially applicable biocatalysts and/or microbial cell factories, a design-build-test-learn cycle-based iterative process is necessary, which is often time-consuming and labor-intensive. The integration of microfluidic technologies into synthetic biology can accelerate these processes, particularly for achieving high-throughput phenotyping and screening. In this review, we examine the potential of microfluidic technologies to revolutionize synthetic biology. Although commercial microfluidics demonstrate superior throughput for single-cell assays, their application can be limited, for example, in cases where products are retained inside the cells. Droplet microfluidics, on the other hand, is a rather flexible platform and shows high diversity in single-cell, cell-to-cell interaction-based, and cell-free reaction-based analyses. By examining previous studies, we have summarized the potential of microfluidic technologies to foster sustainable biomanufacturing and advanced biological engineering.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"1 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green synthesis of gold nanoparticles using Acorus calamus leaf extract and study on their anti-alzheimer potential 利用石菖蒲叶提取物绿色合成金纳米粒子并研究其抗老年痴呆潜力

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-16 DOI: 10.1007/s12257-024-00010-y

Haixia Peng, Shuzhen Zhang, Qiaolian Chai, Zhongchang Hua

{"title":"Green synthesis of gold nanoparticles using Acorus calamus leaf extract and study on their anti-alzheimer potential","authors":"Haixia Peng, Shuzhen Zhang, Qiaolian Chai, Zhongchang Hua","doi":"10.1007/s12257-024-00010-y","DOIUrl":"https://doi.org/10.1007/s12257-024-00010-y","url":null,"abstract":"Nowadays, the fabrication and characterization of plant extract-based gold nanoparticles (AuNPs) as well as their anticancer activities have gained significant attention. Acorus calamus is a typical Chinese medicinal herb that has been utilized since a period of thousand years for treating rheumatism, asthma, tracheitis, etc. In the current study, the reaction parameters are optimized for managing the size of NPs as categorized by the high-resolution transmission electron microscopy. Various characterization methods such as X-ray diffraction, UV-visible spectroscopy and Fourier-transform infrared spectroscopy were carried out to confirm the fabrication of AuNPs mediated by the leaf extract of A. calamus. Further, the neuroprotective potential of fabricated AuNPs was studied by evaluating the cholinesterase inhibitory activity and antioxidant potential. The nanogold aqueous extracts exhibited the highest antioxidant and anti-acetyl cholinesterase activity, confirming that AuNPs can cross the blood-brain barrier and raise the level of acetyl cholinesterase, at the same time reduces the oxidative stress levels. The obtained results concluded their important potential for development of anti-Alzheimer drug having antioxidant and anticholinesterase activity against oxidative stress.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"29 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139757177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering of erythritol production from glycerol by Yarrowia lipolytica 脂肪分解亚罗酵母利用甘油生产赤藓糖醇的代谢工程

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-16 DOI: 10.1007/s12257-024-00005-9

{"title":"Metabolic engineering of erythritol production from glycerol by Yarrowia lipolytica","authors":"","doi":"10.1007/s12257-024-00005-9","DOIUrl":"https://doi.org/10.1007/s12257-024-00005-9","url":null,"abstract":"<h3>Abstract</h3> Erythritol as a four-carbon polyol has been widely used in food, pharmaceutical and daily chemical industries with characteristics of low caloric value and high chemical stability. Here, a system metabolic engineering strategy was used to increase the yield of erythritol from glycerol in Yarrowia lipolytica by enhancing the substrate transformation and restricting the by-product synthesis. Specifically, we determined that over-expression of a newly identified erythrose reductase YPR1 was able to improve the erythritol production as same as the well-known erythrose reductase ER27. Instead of its up-regulation, knockout of erythrose reductase ER10 was effective to improve erythritol synthesis. Moreover, both over-expression of YPR1 and deletion of ER10 significantly accelerated the glycerol utilization in response to high osmotic stress. To further decrease the by-product accumulation, a restriction and recycling strategy was implemented by knockout of mannitol dehydrogenase MDH2 and enhancement of arabitol dehydrogenase ADH1 and fructokinase HXK1. The engineered strain YL13 produced a titer of 25 g/L erythritol and less than 0.5 g/L mannitol and arabitol. By over-expression of transketolase TKL1, the final strain YL14 produced 28.5 g/L erythritol and none of mannitol and arabitol. This study provides a new idea for reducing the production of by-products and improving the glycerol conversion to erythritol.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"222 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive study on the inhibition mechanism of alpha-glucosidase by flavonoids via kinetic and structural analysis 通过动力学和结构分析全面研究黄酮类化合物对α-葡萄糖苷酶的抑制机制

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-15 DOI: 10.1007/s12257-024-00018-4

Jin Young Lee, Hoe-Suk Lee, Yu-Young Lee, Mi-Hyang Kim, Hyun-Joo Kim, Narae Han, Moon Seok Kang, Young Joo Yeon

{"title":"Comprehensive study on the inhibition mechanism of alpha-glucosidase by flavonoids via kinetic and structural analysis","authors":"Jin Young Lee, Hoe-Suk Lee, Yu-Young Lee, Mi-Hyang Kim, Hyun-Joo Kim, Narae Han, Moon Seok Kang, Young Joo Yeon","doi":"10.1007/s12257-024-00018-4","DOIUrl":"https://doi.org/10.1007/s12257-024-00018-4","url":null,"abstract":"Flavonoid consists of an extensive range of compounds with variable inhibitory activities against alpha-glucosidase, implicating its role in the prevention and treatment of diabetes caused by uncontrolled increases in the blood glucose level. Comprehensive study on a selected assortment of flavonoids for their degrees of inhibition, types of inhibitory mode and the structure–function relationship both in terms of the ligand chemical structure and in the context of the enzyme–inhibitor binding complex is therefore necessary to predict and develop efficient flavonoid inhibitors. Herein, flavonoids based on flavone, flavonol, flavanone and isoflavone skeletons in their aglycone and glycone forms were analyzed to this purpose. Most aglycones showed excellent inhibition, while glycones showed relatively low activities. Competitive, noncompetitive and mixed inhibition modes were observed from different types of flavonoids, and their molecular mechanisms by binding to the active or allosteric sites of alpha-glucosidase were analyzed via the docking study. Quercetin with a superior competitive inhibitory activity bound near the catalytic residues within the active site, whereas other glycosylated quercetin derivatives bound at more distal sites. Mixed inhibitors resulted in opposite binding conformations in the allosteric site depending on whether they show more competitive-like or uncompetitive-like behaviors, while the noncompetitive inhibitor could bind in both conformations.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"203 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Galactose-based biohydrogen production from seaweed biomass by novel strain Clostridium sp. JH03 from anaerobic digester sludge 厌氧消化污泥中的新型梭状芽孢杆菌 JH03 利用海藻生物质生产基于半乳糖的生物氢

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-15 DOI: 10.1007/s12257-024-00013-9

Jeong Hyeon Hwang, Hyun Joong Kim, Hyun Jin Kim, Nara Shin, Suk Jin Oh, Jeong-Hoon Park, Won-Dong Cho, Jungoh Ahn, Shashi Kant Bhatia, Yung-Hun Yang

{"title":"Galactose-based biohydrogen production from seaweed biomass by novel strain Clostridium sp. JH03 from anaerobic digester sludge","authors":"Jeong Hyeon Hwang, Hyun Joong Kim, Hyun Jin Kim, Nara Shin, Suk Jin Oh, Jeong-Hoon Park, Won-Dong Cho, Jungoh Ahn, Shashi Kant Bhatia, Yung-Hun Yang","doi":"10.1007/s12257-024-00013-9","DOIUrl":"https://doi.org/10.1007/s12257-024-00013-9","url":null,"abstract":"Seaweed biomass in Korea is rich in galactose following hydrolysis, and leveraging this resource for enhancing the biohydrogen production is the aim of this study. The study investigates the biohydrogen production potential of a newly isolated pure strain, Clostridium sp. JH03, utilizing galactose and seaweed biomass as renewable feedstocks. The strain could utilize galactose as the sole carbon source for biohydrogen production, with a maximum hydrogen yield of 1.61 mol H2/mol galactose. The parameters included pH, temperature, and initial galactose concentration, which were varied to determine the optimal conditions for maximum biohydrogen production. The optimal conditions for biohydrogen production were pH 9 and a temperature of 25 °C, with an initial galactose concentration of 10 g/L. Moreover, hydrogen production from seaweed hydrolysate by Clostridium sp. JH03 resulted in maximum production of 1.71 mol H2/mol galactose. The study also investigated that combining sludge, a common practice in dark fermentation, with JH03 increased biohydrogen production by up to 34%. By addressing the need for clean energy and reducing raw materials price using biomass, this study contributes to the advancement of sustainable and cost-compatible energy solutions.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"16 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymatic synthesis of nylon precursors by 4-aminobutyrate aminotransferase and 6-oxohexanoate dehydrogenase 4-氨基丁酸氨基转移酶和 6-氧代己酸脱氢酶对尼龙前体的酶促合成作用

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-15 DOI: 10.1007/s12257-024-00011-x

Hoe-Suk Lee, Yung-Hun Yang, Young Joo Yeon, Hyun June Park

{"title":"Enzymatic synthesis of nylon precursors by 4-aminobutyrate aminotransferase and 6-oxohexanoate dehydrogenase","authors":"Hoe-Suk Lee, Yung-Hun Yang, Young Joo Yeon, Hyun June Park","doi":"10.1007/s12257-024-00011-x","DOIUrl":"https://doi.org/10.1007/s12257-024-00011-x","url":null,"abstract":"6-Aminocaproic acid and adipic acid are the key value-added chemical precursors in the pharmaceutical, solvent and polyamide industry, including nylon-6, and nylon-6, 6. An enzymatic interconversion of the two precursors can provide a convenient and eco-friendly biosynthetic route to each of the precursors and thus, require analysis of the reaction process. Herein, an in vitro enzymatic method was employed to convert the two precursors while most studies so far have focused on the whole cell bioconversion to investigate the process. 4-Aminobutyrate aminotransferase was utilized to mediate the reactions between 6-aminocaproic acid and the intermediate 6-oxohexanoic acid with the aid of pyridoxal 5’-phosphate and amine donor/acceptor. 6-Oxohexanoate dehydrogenase was utilized for the reaction from 6-oxohexanoic acid to adipic acid with NADP+. A range of reaction conditions were investigated including the type of amine donor, pH conditions, the concentrations of enzyme and amine donor/acceptor. The optimum condition resulted in 78% yield for the reaction from 6-oxohexanoic acid to 6-aminocaproic acid. The yield for the one-pot, two-step enzymatic cascade from 6-aminocaproic acid via 6-oxohexanoate intermediate to adipic acid was 88%, which was higher than the yield for each individual step in the cascade, with 31% and 32%, for the first and second step, respectively. Furthermore, structural analysis on the active site of the 4-aminobutyrate aminotransferase docked with a range of amine donors implicates the optimal donor is glutamate in accordance with the experimental data and suggests enzyme engineering possibilities for more readily available donors to facilitate the industrial application of the process.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"9 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Component analysis and utilization strategy of brown macroalgae as promising feedstock for sugar platform-based marine biorefinery 棕色大型藻类作为基于制糖平台的海洋生物精炼厂的理想原料的成分分析和利用策略

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-14 DOI: 10.1007/s12257-024-00022-8

Jeongho Lee, Hyeonmi Shin, Kang Hyun Lee, Hyeseon Lee, Giwon Lee, Sungho Jang, Gyoo Yeol Jung, Hah Young Yoo, Chulhwan Park

{"title":"Component analysis and utilization strategy of brown macroalgae as promising feedstock for sugar platform-based marine biorefinery","authors":"Jeongho Lee, Hyeonmi Shin, Kang Hyun Lee, Hyeseon Lee, Giwon Lee, Sungho Jang, Gyoo Yeol Jung, Hah Young Yoo, Chulhwan Park","doi":"10.1007/s12257-024-00022-8","DOIUrl":"https://doi.org/10.1007/s12257-024-00022-8","url":null,"abstract":"Brown algae have gained attention as a sustainable feedstock for biorefineries due to their ability to sequester carbon dioxide, rapid growth, and high carbohydrate content. The carbohydrate content in brown algae has only been analyzed for a few species, and in most cases, access to fundamental data such as sugar composition is limited, which hinders the assessment of brown algal biomass-based biorefining potential. In this study, the carbohydrate composition of brown algae (Undaria pinnatifida, Saccharina japonica, Ecklonia cava, and Ecklonia stolonifera) was analyzed in detail and application directions were proposed. As a result, alginate and glucan were detected in all resources, and the contents (alginate and glucan wt%) were as follows: U. pinnatifida (39.6 and 4.9 wt%), S. japonica (34.0 and 6.3 wt%), E. cava (24.3 and 7.7 wt%), and E. stolonifera (39.1 and 9.7 wt%). All feedstocks contain trace amounts (2.9–4.9 wt%) or no xylan-mannan-galactan. Mannitol was detected only in S. japonica (26.7 wt%) in rich, showing high potential as a biorefinery feedstock. We highlight that the carbohydrate composition of E. cava and E. stolonifera was analyzed for the first time and the potential use of brown algal biomass in a biorefinery approach.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"28 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural products ameliorating the adverse health effects by air particulate matter 改善空气颗粒物对健康不利影响的天然产品

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-14 DOI: 10.1007/s12257-024-00012-w

Dongyup Hahn, Min Jeong Kim, Yun Kwon, Eunjeong Kim, Dong Ho Park, Jong-Sup Bae

引用次数: 0