Fabrication of folic acid–cysteamine-modified silver nanoparticles as promising contrast agent for computed tomography imaging

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Wei Lian, Min Gan
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引用次数: 0

Abstract

The present work demonstrates the biosynthesis of silver nanoparticles (AgNPs) using Coffea arabica leaf extract. The AgNPs prepared by green route from C. arabica leaf were characterized through UV–visible, X-ray diffraction, transmission electron microscopy and energy-dispersive electron spectroscopy. Later, the prepared NPs were conjugated with cysteamine–folic acid and utilized as a contrast medium for in vitro targeted imaging of folic acid receptor-expressing malignant cells by computerized tomography (CT). At 80 kVp, the targeted cells exhibited CT values which were two times greater than that of the non-targeted cells. The results were compared with the folic acid-negative cell lines as well as the effective inhibition of folic acid receptor using free folic acid substrate. The outcome of the present study suggests that the fabricated cysteamine–folic acid-conjugated silver nanoparticles could be utilized as a potential contrast agent for molecular CT imaging. This information can be taken into consideration for applying AgNPs in enhancing radiation dose where nanoparticles containing greater X-ray attenuation were applied.

Abstract Image

制备叶酸-半胱胺修饰的银纳米粒子,作为有望用于计算机断层扫描成像的造影剂
本研究利用阿拉伯咖啡叶提取物进行银纳米粒子(AgNPs)的生物合成。通过紫外可见光、X 射线衍射、透射电子显微镜和能量色散电子显微镜对从阿拉伯咖啡叶中提取的绿色方法制备的 AgNPs 进行了表征。随后,制备的 NPs 与半胱胺-叶酸共轭,用作计算机断层扫描(CT)对叶酸受体表达的恶性细胞进行体外靶向成像的造影剂。在 80 kVp 下,靶细胞的 CT 值是非靶细胞的两倍。研究结果与叶酸阴性细胞系以及使用游离叶酸底物有效抑制叶酸受体的结果进行了比较。本研究结果表明,制备的半胱胺-叶酸共轭银纳米粒子可作为一种潜在的造影剂用于分子 CT 成像。在应用含更大 X 射线衰减的纳米粒子提高辐射剂量时,可以考虑这一信息。
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来源期刊
Biotechnology and Bioprocess Engineering
Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物
CiteScore
5.00
自引率
12.50%
发文量
79
审稿时长
3 months
期刊介绍: Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
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