Biotechnology and Bioprocess Engineering最新文献_第9页

Strategies for the enhancement of anti-cancer effect of phosphodiesterase type 5 inhibitors using drug binding fusion proteins 利用药物结合融合蛋白增强 5 型磷酸二酯酶抑制剂抗癌效果的策略

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-13 DOI: 10.1007/s12257-024-00014-8

Hongbin Kim, Chang Yeop Keum, Su Yeon Lim, Kwang Suk Lim

{"title":"Strategies for the enhancement of anti-cancer effect of phosphodiesterase type 5 inhibitors using drug binding fusion proteins","authors":"Hongbin Kim, Chang Yeop Keum, Su Yeon Lim, Kwang Suk Lim","doi":"10.1007/s12257-024-00014-8","DOIUrl":"https://doi.org/10.1007/s12257-024-00014-8","url":null,"abstract":"Drug repurposing is a rapidly growing for the development of new therapeutic agents. Among them, phosphodiesterase-5 (PDE5) inhibitors, which show excellent effects as a treatment for erectile dysfunction, have been continuously reported for their potential as anti-cancer agents. It is necessary to apply targeted delivery systems to enhance the efficacy of the PDE5 inhibitors (PDE5i) as anti-cancer drugs. In this study, PDE5 inhibitor-binding domain (PBD) was developed to incorporate PDE5i into antibody using biological affinity binding without any chemical modifications. The PBD spontaneously bound with PDE5i, resulting in the formation of PBD/PDE5i complex. We also constructed a recombinant fusion protein composed of an anti-HER2 scFv and PBD (HER2 scFv-PBD) to deliver the PDE5i for the treatment of HER2 positive cancer cells. HER2 scFv-PBD could deliver the PDE5i into HER2-positive cancer cells and enhanced anti-cancer effect of the PDE5i. We have demonstrated the potential of tumor-directed PDE5 inhibition as a novel form of targeted therapy using drug binding fusion proteins.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"10 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical, structural characterization and assessing the biological effects of cinnamon nanoparticles 肉桂纳米粒子的生物化学、结构特征和生物效应评估

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-13 DOI: 10.1007/s12257-024-00004-w

Zahra Sabouri, Neda Shakour, Mohammad Sabouri, Samaneh Sadat Tabrizi Hafez Moghaddas, Majid Darroudi

{"title":"Biochemical, structural characterization and assessing the biological effects of cinnamon nanoparticles","authors":"Zahra Sabouri, Neda Shakour, Mohammad Sabouri, Samaneh Sadat Tabrizi Hafez Moghaddas, Majid Darroudi","doi":"10.1007/s12257-024-00004-w","DOIUrl":"https://doi.org/10.1007/s12257-024-00004-w","url":null,"abstract":"The natural polyphenolic materials that cinnamon contains are well known for their different biological applications and have a wide variety of pharmacological and therapeutic attributes. Bioactive and harmless cinnamon nanoparticles (cinnamon-NPs) can be anticancer and antidiabetic agents. For this purpose, water-soluble cinnamon-NPs were synthesized using the hydrothermal technique for the first time and in vitro studies were performed to investigate their anti-diabetic effects. The outcomes of morphology, size, and stability of cinnamon-NPs were described through Transmission electron microscopy (TEM), X-ray diffraction (XRD), Dynamic light scattering/Zeta (DLS/Zeta), Ultraviolet–visible (UV–Vis), and Fourier transform infrared (FTIR) analyses. The morphology of cinnamon-NPs was spherical and their average size was about 14.8 nm. Furthermore, the glucose consumption assay results revealed that compound cinnamon-NPs at 1.0 and 10 µM significantly lowered glucose levels (p < 0.05) in HepG2 cells exposed to 11.0 and 22.0 mM of glucose compared with standard (pioglitazone) and control groups. Therefore, the utilization of spherical NPs suspended in water could be useful for investigating therapeutic applications.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"22 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colorimetric detection of milk spoilage at low temperatures: a novel PDA/ZnO@NC membrane for pH-based freshness monitoring 低温下牛奶腐败的比色检测：基于 pH 值的新鲜度监测用新型 PDA/ZnO@NC 膜

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-13 DOI: 10.1007/s12257-024-00021-9

Zhaowei Li, Huisoo Jang, Sungjin Park, Sun Min Kim, Tae-Joon Jeon

{"title":"Colorimetric detection of milk spoilage at low temperatures: a novel PDA/ZnO@NC membrane for pH-based freshness monitoring","authors":"Zhaowei Li, Huisoo Jang, Sungjin Park, Sun Min Kim, Tae-Joon Jeon","doi":"10.1007/s12257-024-00021-9","DOIUrl":"https://doi.org/10.1007/s12257-024-00021-9","url":null,"abstract":"Food waste is a huge problem worldwide, and spoilage during storage is a major contributor. Food freshness monitoring systems can help reduce food waste by providing consumers with information to more accurately predict the shelf life of their food. In the case of milk, pH can be used as a freshness indicator. As milk spoils, microbial activity converts lactose to lactic acid, which produces hydrogen ions (H+) and consequently lowers the pH of the milk. In this paper, we propose a paper sensor that can detect the pH of milk in a refrigerated state (− 4 ℃) by color change. Polydiacetylene (PDA)/zinc oxide (ZnO) nanocomposite, which changes its color from blue to red as the pH decreases, was used as the color changing material. Specifically, the nanocomposite was applied to a nitrocellulose (NC) membrane, which is approved as a food packaging material for monitoring freshness during transport or on the shelf. The freshness monitoring function of the sensor was verified by applying artificially spoiled milk samples to the PDA/ZnO@NC membrane. In particular, it was demonstrated that the PDA/ZnO@NC membrane was shown to detect spoiled milk samples with pH 4.5 in 10 min at low temperature. Furthermore, the color change of the sensor upon detection of spoilage remained stable even after long-term storage. In conclusion, the PDA/ZnO@NC membrane presented in this study can be widely applied to the freshness monitoring of various food products stored at low temperatures whose spoilage is evaluated by pH.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"3 2 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of folic acid–cysteamine-modified silver nanoparticles as promising contrast agent for computed tomography imaging 制备叶酸-半胱胺修饰的银纳米粒子，作为有望用于计算机断层扫描成像的造影剂

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-13 DOI: 10.1007/s12257-024-00017-5

Wei Lian, Min Gan

{"title":"Fabrication of folic acid–cysteamine-modified silver nanoparticles as promising contrast agent for computed tomography imaging","authors":"Wei Lian, Min Gan","doi":"10.1007/s12257-024-00017-5","DOIUrl":"https://doi.org/10.1007/s12257-024-00017-5","url":null,"abstract":"The present work demonstrates the biosynthesis of silver nanoparticles (AgNPs) using Coffea arabica leaf extract. The AgNPs prepared by green route from C. arabica leaf were characterized through UV–visible, X-ray diffraction, transmission electron microscopy and energy-dispersive electron spectroscopy. Later, the prepared NPs were conjugated with cysteamine–folic acid and utilized as a contrast medium for in vitro targeted imaging of folic acid receptor-expressing malignant cells by computerized tomography (CT). At 80 kVp, the targeted cells exhibited CT values which were two times greater than that of the non-targeted cells. The results were compared with the folic acid-negative cell lines as well as the effective inhibition of folic acid receptor using free folic acid substrate. The outcome of the present study suggests that the fabricated cysteamine–folic acid-conjugated silver nanoparticles could be utilized as a potential contrast agent for molecular CT imaging. This information can be taken into consideration for applying AgNPs in enhancing radiation dose where nanoparticles containing greater X-ray attenuation were applied.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"42 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Tat-fused drug binding protein to improve anti-cancer effect of mammalian target of rapamycin inhibitors 开发与 Tat 融合的药物结合蛋白，提高哺乳动物雷帕霉素靶点抑制剂的抗癌效果

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-13 DOI: 10.1007/s12257-024-00015-7

Su Yeon Lim, Sugyeong Kim, Hongbin Kim, Hyun-Ouk Kim, Suk-Jin Ha, Kwang Suk Lim

{"title":"Development of Tat-fused drug binding protein to improve anti-cancer effect of mammalian target of rapamycin inhibitors","authors":"Su Yeon Lim, Sugyeong Kim, Hongbin Kim, Hyun-Ouk Kim, Suk-Jin Ha, Kwang Suk Lim","doi":"10.1007/s12257-024-00015-7","DOIUrl":"https://doi.org/10.1007/s12257-024-00015-7","url":null,"abstract":"The mammalian target of rapamycin (mTOR) is known to regulate cell growth, protein stability and cell-cycle progression, and many human tumors result from the dysregulation of mTOR signaling. Although various mTOR inhibitors have been developed, effective delivery systems are still needed to enhance the anti-cancer effects of mTOR inhibitors. In this study, we developed the Tat-fused mTOR inhibitor binding domain (Tat-MBD/TMBD) for the enhancement of the anti-cancer effect of mTOR inhibitors, due to the improvement of intracellular uptake. A TMBD/mTOR inhibitors complex spontaneously formed by biological affinity between MBD and mTOR inhibitors without chemical conjugation and modification. We constructed that a recombinant fusion protein expression vector composed of Tat (protein transduction domain) and mTOR inhibitor-binding domain (Tat-MBD) to deliver the mTOR inhibitors. The MBD spontaneously bound with mTOR inhibitors including sirolimus, everolimus, and temsirolimus, resulting in the formation of a TMBD/mTOR inhibitors complex. The enhancement of the delivery efficacy of mTOR inhibitors into various breast cancer cells was confirmed and improved anti-cancer efficacy was observed. We demonstrated the effective delivery systems of mTOR inhibitors without chemical conjugation of mTOR inhibitors.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"11 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of pulsed dielectrophoretic-field flow fractionation separation coupled with fibre-optic detection on a lab-on-chip as a technique to separate similar bacteria cells 在片上实验室评估脉冲介电泳-场流分馏分离和光纤检测技术，作为一种分离相似细菌细胞的技术

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-13 DOI: 10.1007/s12257-024-00001-z

Mohd Firdaus Kamuri, Zurina Zainal Abidin, Mohd Hanif Yaacob, Mohd Nizar Hamidon

{"title":"Assessment of pulsed dielectrophoretic-field flow fractionation separation coupled with fibre-optic detection on a lab-on-chip as a technique to separate similar bacteria cells","authors":"Mohd Firdaus Kamuri, Zurina Zainal Abidin, Mohd Hanif Yaacob, Mohd Nizar Hamidon","doi":"10.1007/s12257-024-00001-z","DOIUrl":"https://doi.org/10.1007/s12257-024-00001-z","url":null,"abstract":"This study addresses the challenge of separating bacteria with similar structures such as Escherichia coli and Aeromonas hydrophila. This approach employs pulsed field dielectrophoresis assisted by laminar flow fractionation in a lab-on-a-chip system with integrated optical detection. Bacterial cells passed through 30-µm microelectrodes subjected at 1 MHz and 14 V peak-to-peak in pulsed mode, while fluid flow carried bacteria towards the chamber’s end. The on-and-off electric field at specific pulse intervals expose bacterial cells to diverse forces, including kinetics, dielectrophoresis, gravity, drag, and diffusion, resulting in a net force facilitating their movement. Variations of pulsing time, flow rates, and voltage were investigated to identify the optimal combination for efficient separation. Next, the bacteria were detected using an optical fibre based on their absorbance. Results demonstrated a 30% separation efficiency in 90 min at 9.6 μL min−1 flow rates, 4 s pulsing time, and 40 μS cm−1 medium conductivity. A. hydrophila aggregates experienced greater DEP force and retained at microelectrodes during electric field application compared to E. coli, which moved faster towards optical detection. The separation mechanism with and without electric field was different, and precise control of cell movement during field-off periods is important to minimize uncontrolled diffusion. While the optical detection part has been successful, longer time and separation length are recommended for better separation. A carefully tuned combination of pulsing time, flow rates, voltage, and microelectrode design is crucial for this integrated lab-on-chip system to be efficient for separating and detecting closely related microorganisms.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"3 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reconstruction of genome-scale metabolic models of non-conventional yeasts: current state, challenges, and perspectives 非常规酵母菌基因组尺度代谢模型的重建：现状、挑战和前景

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-13 DOI: 10.1007/s12257-024-00009-5

Eduardo Luís Menezes de Almeida, Eduard J. Kerkhoven, Wendel Batista da Silveira

{"title":"Reconstruction of genome-scale metabolic models of non-conventional yeasts: current state, challenges, and perspectives","authors":"Eduardo Luís Menezes de Almeida, Eduard J. Kerkhoven, Wendel Batista da Silveira","doi":"10.1007/s12257-024-00009-5","DOIUrl":"https://doi.org/10.1007/s12257-024-00009-5","url":null,"abstract":"Non-conventional yeasts are promising cell factories to produce lipids and oleochemicals, metabolites of industrial interest (e.g., organics acids, esters, and alcohols), and enzymes. They can also use different agro-industrial by-products as substrates within the context of a circular economy. Some of these yeasts can also comprise economic and health burdens as pathogens. Genome-scale metabolic models (GEMs), networks reconstructed based on the genomic and metabolic information of one or more organisms, are great tools to understand metabolic functions and landscapes, as well as propose engineering targets to improve metabolite production or propose novel drug targets. Previous reviews on yeast GEMs have mainly focused on the history and the evaluation of Saccharomyces cerevisiae modeling paradigms or the accessibility and usability of yeast GEMs. However, they did not describe the reconstruction strategies, limitations, validations, challenges, and research gaps of non-conventional yeast GEMs. Herein, we focused on the reconstruction of available non-Saccharomyces GEMs, their validation, underscoring the physiological insights, as well as the identification of both metabolic engineering and drug targets. We also discuss the challenges and knowledge gaps and propose strategies to boost their use and novel reconstructions.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"46 11 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced fermentative γ-aminobutyric acid production by a metabolic engineered Corynebacterium glutamicum 经代谢工程改造的谷氨酸棒状杆菌提高了γ-氨基丁酸的发酵产量

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-12 DOI: 10.1007/s12257-024-00008-6

{"title":"Enhanced fermentative γ-aminobutyric acid production by a metabolic engineered Corynebacterium glutamicum","authors":"","doi":"10.1007/s12257-024-00008-6","DOIUrl":"https://doi.org/10.1007/s12257-024-00008-6","url":null,"abstract":"<h3>Abstract</h3> γ-Aminobutyric acid (GABA) is a non-proteinogenic amino acid with important physiological functions, which has been widely used in food, pharmaceuticals, and polyamides production. The fermentative GABA production by Corynebacterium glutamicum was recognized as one of the most promising methods. However, the problems of low catalytic activity of the heterologously expressed glutamate decarboxylase (GAD) and the imbalanced carbon flux between cell growth and GABA synthesis severely limited the GABA production by C. glutamicum. This study applied combinational metabolic engineering and catalytic condition optimization strategies to solve these two major obstacles. The secretory expression of GAD was enhanced using a bicistronic-designed expression cassette. This bicistronic expression cassette was further triply inserted into the genome by substituting the ldhA, pqo, and ack genes, thus stabilizing the expression of GAD and reducing the accumulation of by-products of lactate and acetate. A growth-regulated promoter PCP_2836 was applied to dynamically control the expression of odhA, thus controlling the α-oxoglutarate dehydrogenase complex activity for balanced cell growth and GABA production. The glutamate precursor synthesis and pyridoxal 5′-phosphate supply were also strengthened by promoter substitution. Finally, through a two-stage pH-controlled fed-batch fermentation under optimized conditions, the engineered strain reached GABA titer of 81.31 ± 1.31 g/L with a yield and productivity of 0.50 ± 0.01 g/g and 1.36 ± 0.23 g L−1 h−1, which was 4.8%, 13.6%, and 11.2% higher than that of the original strain. This study laid a solid foundation for industrial fermentative GABA production by engineered C. glutamicum.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"168-169 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of a novel bioprocess for the cultivation Synechococcus nidulans on Mars on its biochemical composition: focus on the lipidome 用于在火星上培养 Synechococcus nidulans 的新型生物工艺对其生化组成的影响：重点关注脂质组

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-12 DOI: 10.1007/s12257-024-00019-3

Mattia Casula, Giacomo Fais, Agnieszka Sidorowicz, Pierluigi Caboni, Giacomo Cao, Alessandro Concas

{"title":"Effects of a novel bioprocess for the cultivation Synechococcus nidulans on Mars on its biochemical composition: focus on the lipidome","authors":"Mattia Casula, Giacomo Fais, Agnieszka Sidorowicz, Pierluigi Caboni, Giacomo Cao, Alessandro Concas","doi":"10.1007/s12257-024-00019-3","DOIUrl":"https://doi.org/10.1007/s12257-024-00019-3","url":null,"abstract":"In the present work, the possibility to grow the strain Synechococcus nidulans CCALA 188 on Mars using a medium mimicking a one obtainable using in situ available resources, i.e. the so-called Martian medium, under an atmosphere obtainable by pressurization of Mars CO2, is investigated. The goal is to obtain a biomass with high-value products to sustain a crewed mission to Mars. The results show that the replacement of 40% vol of Z-medium with the same volume of Martian medium does not affect the cultivation and leads to a slight improvement of biomass productivity. Under an atmosphere consisting of pure CO2 the growth rate was reduced but the strain managed to adapt by modifying its metabolism. Total proteins and carbohydrates were significantly reduced under Mars-like conditions, while lipids increased when using CO2. A balanced diet rich in antioxidants is crucial for the wealth of astronauts, and in our case, radical scavenging capacities range from 15 to 20 mmolTEAC/kg were observed. Under CO2, a reduction in antioxidant power is observed likely due to a decrease in photosynthetic activity. The lipidome consisted of sulfoquinovosyldiacylglycerol, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylcholine, phosphatidylglycerol, and triacylglycerol. A significant increase in the latter ones was observed under Mars simulated atmosphere.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"3 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xylanase enzyme from novel strain and its immobilization onto metal organic framework MOF for fruit juice clarification 来自新型菌株的木聚糖酶及其在金属有机框架 MOF 上的固定化用于果汁澄清

IF 3.2 4区生物学

Biotechnology and Bioprocess Engineering Pub Date : 2024-02-12 DOI: 10.1007/s12257-024-00007-7

Jyoti Kaushal, Madhu Khatri, Gursharan Singh, Shailendra Kumar Arya

{"title":"Xylanase enzyme from novel strain and its immobilization onto metal organic framework MOF for fruit juice clarification","authors":"Jyoti Kaushal, Madhu Khatri, Gursharan Singh, Shailendra Kumar Arya","doi":"10.1007/s12257-024-00007-7","DOIUrl":"https://doi.org/10.1007/s12257-024-00007-7","url":null,"abstract":"Bacillus pumilus, a bacterial strain was isolated from agricultural soil and used for xylanase enzyme (Xy) production under the submerged fermentation technique. The (Xy) enzyme had an optimum temperature at 50℃ (maximum activity from 45–60 °C) and was active at broad pH range (5.0–8.0) with an optimum pH at around 6.3 as evaluated from response surface methodology studies. This enzyme after purification (purification; 2.87 folds, specific activity; 64.3 U/mg) was immobilized onto MOFCu-BTC (a copper ion-based metal organic framework) and was used for clarification of freshly squeezed fruit juice (pineapple and pomegranate). The study revealed an improved catalytic efficiency (Vmax from 1.252.5 to 1.361 U/mL/mg of support) and greater half-life of the immobilized system (77–99 min). The activation energy decreased from that required for the free system (37.59–25.63 kJ/mol). The reusability of the enzyme improved after immobilization where 61% of the enzyme’s activity was retained after 21 cycles of usage. The MOFXy-Cu-BTC system showed improved clarification (47.58–57.97% for pineapple, and 15.34–18.3 for pomegranate) thereby showing its effectiveness in commercial juice clarification process.","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"31 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0