开发和评估基于轨道摇摆运动的一次性使用细胞培养系统 CELBIC® 系统

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Hyunwoo Kim, Seohyun Park, Rock Ki Kim, Yeong Ok Baik, Kyung Nam Kim, Duk Jae Oh
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引用次数: 0

摘要

在生物制药生产的研究和工业领域,一次性使用生物反应器(SUB)的重要性与日俱增。本研究介绍了一种新开发的一次性生物反应器系统 CELBIC®,该系统采用了独特的搅拌方法,并对其物理性质和生物应用进行了评估。在 CELBIC® 系统中使用 CHO-DG44 和 CHO-S 两种中国仓鼠卵巢(CHO)细胞系进行了 1 升和 50 升规模的生物评估,结果显示细胞培养性能与搅拌罐型生物反应器相似。这些结果支持 CELBIC® 系统成为适用于生物制药生产的细胞培养的新型 SUB 系统。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Development and evaluation of orbital rocking motion-based single-use cell culture system, the CELBIC® system

Development and evaluation of orbital rocking motion-based single-use cell culture system, the CELBIC® system

The importance of a single-use bioreactor (SUB) is continuously increasing in research and industrial fields for biopharmaceutical production. In this study, a newly developed SUB system, the CELBIC® system, applied with the unique agitation method is introduced, and its physical properties and biological applications are evaluated. The mixing time was in the range of 11.0–129.0 s, and the volumetric mass transfer coefficient, kLa, measured through surface aeration, was in the range of 2.7–15.5 h−1 at a working volume of 1–10 L. Biological evaluations using two Chinese hamster ovary (CHO) cell lines, CHO-DG44 and CHO-S cell, were carried out in the CELBIC® systems in 1 and 50 L scales, showing similar cell culture performance as that from stirred tank-type bioreactors. These results support the CELBIC® system as a new SUB system applicable to cell cultures for biopharmaceutical production.

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来源期刊
Biotechnology and Bioprocess Engineering
Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物
CiteScore
5.00
自引率
12.50%
发文量
79
审稿时长
3 months
期刊介绍: Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
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