Biological Trace Element Research最新文献

{"title":"Metal(loid) Occurrence, Possible Source Patterns, and Screening-Level Human Health Risk in Dried Fish from Thailand.","authors":"Pannraphat Takolpuckdee, Wattanee Boonwittaya, Somkid Tangkan","doi":"10.1007/s12011-026-05241-z","DOIUrl":"10.1007/s12011-026-05241-z","url":null,"abstract":"<p><p>Metal(loid)s are contaminants frequently detected in processed foods. This study investigated contamination of metal(loid)s, including arsenic, cadmium, chromium, copper, manganese, nickel, and zinc, in 8 types of dried fish obtained from major distribution sources supplying products nationwide in Thailand. In addition, relationships among metal(loid)s were examined to interpret possible source patterns. A screening-level health risk assessment was conducted using deterministic methods based on United States Environmental Protection Agency methodology and probabilistic approaches employing Monte Carlo simulation. Maximum allowable ingestion rates for populations in 9 regions of Thailand were also estimated. The mean concentrations of metal(loid)s followed the order: copper > zinc > nickel > manganese > arsenic > chromium > cadmium. Arsenic, copper, and manganese exceeded selected reference standards. Statistical analysis suggested that contamination may be associated with aquatic environments and processing practices. Deterministic risk assessment based on Hazard Index and Total Carcinogenic Risk indicated that all population groups were estimated to exceed acceptable thresholds for non-carcinogenic and carcinogenic effects at the screening level. In several regions, consumption rates exceeded maximum allowable ingestion levels, potentially increasing health risks, particularly for arsenic, copper, chromium, and nickel under conservative assumptions. Probabilistic assessment showed that high-exposure estimates represented by the 95th percentile were likely to exceed acceptable thresholds for both non-carcinogenic and carcinogenic effects across all regions. These findings highlight the need for coordinated risk mitigation strategies across the dried fish production chain to reduce potential dietary exposure to metal(loid)s and support food safety management.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"8023-8045"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Dietary Nano-Selenium on Growth, Selenoprotein Gene Expression, and Antioxidant Adaptation in Pot-Bellied Seahorse (Hippocampus abdominalis).","authors":"Yi Zhang, Jing Zhang, Shuai Liang, Kai-Hua Geng, Tian-Yu Lu, Tian Lan, Dong-Jie Shen, Hui-Long Qiu, Shi-Gen Ye, Jia-Qiang Huang, Guo-Jun Yang, Lian-Shun Wang","doi":"10.1007/s12011-026-05323-y","DOIUrl":"https://doi.org/10.1007/s12011-026-05323-y","url":null,"abstract":"<p><strong>Background: </strong>The pot-bellied seahorse (Hippocampus abdominalis) is a high-value marine teleost, but its responses to dietary selenium (Se) remain poorly characterized.</p><p><strong>Methods: </strong>This 70-day trial evaluated nominal nano-selenium (Nano-Se) supplementation levels of 0, 10, 20, and 40 mg/kg; the unsupplemented frozen mysis shrimp contained 0.97 mg Se/kg on a wet-weight basis.</p><p><strong>Results: </strong>Compared with the control, body weight gain and specific growth rate were higher, and feed conversion ratio was lower at 20 and 40 mg/kg. Additionally, body length gain was increased at 10 and 40 mg/kg. Tissue Se concentrations increased with supplementation and were highest in the liver. Nano-Se increased intestinal villus height and digestive enzyme activities; no overt histopathological lesions were observed in the examined sections. Intestinal 16S rRNA gene sequencing identified changes in microbial diversity and taxonomic composition. Untargeted lipidomics identified semi-quantitative changes in hepatic triglyceride-, phospholipid-, and ether-lipid-associated ion signals. Hepatic superoxide dismutase, catalase, and glutathione peroxidase activities were lower in supplemented groups than in the control. Catalase and superoxide dismutase activities were higher at 40 than at 20 mg/kg, whereas glutathione peroxidase did not differ between doses. Steady-state messenger RNA abundances of selenoprotein-related genes and whole-body sex steroid concentrations also changed.</p><p><strong>Conclusions: </strong>Overall, Nano-Se elicited dose- and endpoint-dependent responses, with no single dose consistently superior. The 10-20 mg/kg range warrants further evaluation, whereas 40 mg/kg maximized Se accumulation. These findings provide a basis for refining Nano-Se dose selection in seahorse aquaculture, although the optimal inclusion level and upper safe limit remain unresolved.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Protective Effects of Selenium Nanoparticles Against Metal Toxicity.","authors":"Anatoly V Skalny, Michael Aschner, Tatiana V Korobeinikova, Fernando Barbosa, Ji-Chang Zhou, Ekaterina V Silina, Victor A Stupin, Tianfeng Chen, Joao B T Rocha, Alexey A Tinkov","doi":"10.1007/s12011-026-05210-6","DOIUrl":"10.1007/s12011-026-05210-6","url":null,"abstract":"<p><p>The objective of the present review is to discuss the protective effects of SeNPs against metal toxicity with special emphasis on the underlying mechanisms and efficacy of SeNPs in comparison to other Se species. In vivo and in vitro studies show that SeNPs mitigate the adverse effects of metal(loid)s (cadmium, lead, arsenic, aluminum, etc.), metal nanoparticles (silver, cobalt), as well as platinum-based agent cisplatin in brain, liver, kidneys, heart, intestine, and gonads. SeNPs substantially reduce toxic metal accumulation in the organism, thus attenuating their toxicity. In metal-exposed animals, SeNPs enhance the biosynthesis of selenoproteins (including GPX4) and up-regulate Nrf2 signaling, thus inhibiting oxidative stress and ferroptosis. Anti-inflammatory effect of SeNPs was shown to be mediated by inhibition of NF-κB and MAPKs, as well as activation of PI3K/Akt signaling. Inhibition of metal-induced endoplasmic reticulum stress and improvement of autophagy also underlies the protective effects of SeNPs. Neuroprotective effects of SeNPs were shown to be mediated by inhibition of metal-induced increase in amyloid β and Tau protein accumulation and aggregation. The majority of studies show that SeNPs possess stronger protective effect against metal toxicity compared to sodium selenite or organoselenium compounds. Furthermore, the beneficial effects of SeNPs in metal-exposed animals were significantly increased by combined administration or surface modifications with bioactive molecules. Taken together, these findings show that SeNPs possess protective effects against metal toxicity through a variety of mechanisms, although their potential efficacy in treatment metal intoxication in humans has yet to be determined.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7592-7624"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148374326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supranutritional Selenomethionine During Different Trimesters of Gestation in Beef Cows Alters Gene Expression Patterns in Peripheral Blood Mononuclear Cells at Parturition.","authors":"Gerd Bobe, Joseph J Klopfenstein, T Zane Davis, Jean A Hall","doi":"10.1007/s12011-026-05213-3","DOIUrl":"10.1007/s12011-026-05213-3","url":null,"abstract":"<p><p>Selenium (Se) is an essential micronutrient. We have previously shown that supranutritional Se-yeast supplementation improves growth and immune function in Se-replete cattle and sheep despite limited impact on selenoprotein and oxidoreductase gene expression, suggesting effects beyond the selenocysteine proteome. This study evaluated the impact of supranutritional Se-yeast during each trimester of pregnancy on transcriptome-wide gene expression in peripheral blood mononuclear cells (PBMCs) at parturition, and the underlying functional categories affected. We administered Se-yeast containing boluses weekly (105 mg Se/wk for 13 wks; 5× the level recommended by NASEM) to crossbred Angus cows during the first (TR1), second (TR2), or third (TR3) trimester of gestation. Control cows (CTR) had continuous access to 120 mg Se/kg mineral supplement as Na selenite (per US FDA regulations) throughout gestation. Blood was collected at parturition, and PBMCs were isolated for RNA sequencing. We identified 3,904 unique differentially expressed genes (DEGs), with 186 upregulated vs. 268 downregulated DEGs across all three Se-yeast TR vs. CTR comparisons, while selenoprotein and oxidoreductase gene expression remained unchanged. Downregulation increased across GO categorization levels, from balanced changes in Cellular Component terms (51 upregulated vs. 52 downregulated) through Molecular Function (13 vs. 139) and Biological Process terms (23 vs. 595), then decreased for KEGG pathways (28 vs. 76) and protein class terms (6 vs. 16). We hypothesize that supranutritional Se-yeast acts primarily through nonspecific SeMet incorporation into the broader proteome rather than through specific selenoprotein synthesis. These PBMC DEG changes may also affect disease risk in periparturient beef cattle, though further investigation is warranted to test these hypotheses.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7655-7676"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148496972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution Patterns and Accumulation Factors of Rare Earth Elements in the Organism of Manchurian Wapiti and Wild Boar in Primorsky Krai, Russian Far East.","authors":"Baranovskaya Natalia, Panichev Alexander, Strepetov Dmitry, Seryodkin Ivan, Chekryzhov Igor, Makarevich Raisa, Ageeva Elena, Slepushkina Anastasia","doi":"10.1007/s12011-026-05221-3","DOIUrl":"10.1007/s12011-026-05221-3","url":null,"abstract":"<p><p>This study presents a comparative analysis of rare earth element (REE) distribution in 24 organs and tissues of four Manchurian wapiti (Cervus canadensis xanthopygus) and four wild boar (Sus scrofa) harvested near the borders of five protected natural areas in Primorsky Krai, Russian Far East. A total of 192 biological samples (96 per species) were analyzed using ICP-MS. At the level of all analyzed organ and tissue samples, statistically significant interspecific differences (p = 0.004) were observed only for europium (Eu), with wapiti showing the most pronounced Eu anomaly in bone tissue (δEu = 190 compared to 52 in wild boar). Analysis of REE concentrations among individual organs and tissues revealed species-specific accumulation patterns: in Manchurian wapiti, REE are deposited primarily in hair and intestine, whereas in wild boar, the liver serves as an additional major depot alongside hair. Elevated concentrations of heavy REE were detected in wild boar lung tissue. The presence of REE in the central nervous system of both species suggests that these elements can reach neural tissues, raising questions about their potential neurotoxicity. These results indicate that interspecific differences in REE accumulation and distribution may be related to behavioral and physiological factors, including dietary habits, digestive system type, and species-specific target organs. This study provides data and potential insights for understanding REE behavior in the body of terrestrial mammals.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7762-7774"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148374261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prenatal Chromium Exposure and Gestational Diabetes Mellitus: Integrating Genetic and Epigenetic Regulation of LPCAT1 from a Phospholipid Remodeling Perspective.","authors":"Yuxin Tian, Lu Yao, Jiaqi Zhang, Meng Zhou, Yue Dou, Bole Zhang, Yulin Li, Yongliang Feng, Yawei Zhang, Suping Wang, Weiwei Wu","doi":"10.1007/s12011-026-05205-3","DOIUrl":"10.1007/s12011-026-05205-3","url":null,"abstract":"<p><p>The trace element chromium (Cr) has been implicated in the risk of gestational diabetes mellitus (GDM), the underlying mechanisms remain unclear. Phospholipid remodeling is known to contribute to insulin regulation and may serve as a potential intermediate link between Cr exposure and GDM risk. Lysophosphatidylcholine acyltransferase 1 (LPCAT1), as an important regulatory factor in phospholipid remodeling, may play a certain role in this process. For the first time, this study investigates the relationship between Cr exposure and GDM risk from the perspective of phospholipid remodeling, while integrating the genetic predisposition and epigenetic regulation of the LPCAT1 gene. A cohort comprising 107 pregnant women diagnosed with GDM and 107 matched controls without GDM was recruited. Blood samples were collected prior to delivery to perform LPCAT1 genotyping, assess DNA methylation patterns, and quantify Cr concentrations. Elevated Cr levels in maternal blood were significantly correlated with an increased risk of GDM. Furthermore, a multiplicative interaction was observed between Cr exposure and four single nucleotide polymorphisms (SNPs) within the LPCAT1 gene. Three Cr-associated DNA methylation CpG sites were identified in GDM patients. Methylation quantitative trait locus (mQTLs) analysis revealed that three SNPs (rs4371798, rs56358072, rs6554859) were significantly associated with methylation at a specific CpG site (cg22185977) within LPCAT1. These findings suggest that genetic polymorphisms in LPCAT1 may modulate individual susceptibility to Cr exposure and consequent GDM risk by influencing the methylation status of the LPCAT1 gene. This study provides novel etiological insights into the role of Cr in inducing GDM through mechanisms involving phospholipid remodeling.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7523-7533"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148343871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disruption of Elemental Homeostasis and Oxidative Balance in Breast Cancer Patients: The Role of Heavy Metals and Trace Elements.","authors":"Zeynep Bozkoyun Dusak, Nurhayat Atasoy, Halit Demir, Canan Demir, Mehmet Naci Aldemir","doi":"10.1007/s12011-026-05208-0","DOIUrl":"10.1007/s12011-026-05208-0","url":null,"abstract":"<p><p>Breast cancer is the most frequently diagnosed malignancy among women globally, accounting for approximately 2.3 million new cases and 670,000 deaths in 2022. Trace element imbalance, heavy metal exposure, and oxidative stress play critical roles in the pathogenesis of breast cancer (BC). This study aimed to determine the serum concentrations of total sulfhydryl (TSH) groups-a marker of non-enzymatic antioxidant capacity distinct from thyroid-stimulating hormone-ischemia-modified albumin (IMA), essential trace elements (Zn, Mg, Cu, Mn, Fe), and toxic heavy metals (Cd, Pb) in newly diagnosed BC patients before initiating chemotherapy, and to compare these parameters with healthy individuals. The study was conducted at Van Yüzüncü Yıl University Dursun Odabaş Medical Center and included 30 newly diagnosed BC patients and 30 healthy volunteers. Blood samples were collected into trace element-grade anticoagulant-free serum tubes. Serum levels of trace elements and heavy metals were analyzed using Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES, Thermo Scientific iCAP 6900). TSH levels were measured using the DTNB (Ellman) method, and IMA levels were assessed colorimetrically. Statistical analyses were performed using SPSS 19.0; normality was assessed by the Shapiro-Wilk test; group comparisons employed the Mann-Whitney U test, with a significance level set at p < 0.05. The results demonstrated that serum concentrations of Cu, Fe, Mg, Mn, and Zn were significantly lower in the patient group compared to controls (p < 0.001). Conversely, levels of Cd and Pb were significantly higher in BC patients (p < 0.001). IMA levels were significantly increased (44.71 ± 4.03 vs. 22.52 ± 0.27 ABSU; p < 0.001), while TSH levels were significantly decreased (1.58 ± 0.45 vs. 3.26 ± 0.05 mmol/L; p < 0.001). Spearman correlation analysis identified a significant positive association between serum Zn and IMA within the patient group (r = 0.493, p = 0.007). These findings indicate that breast cancer is associated with disrupted mineral homeostasis, increased toxic metal accumulation, and impaired antioxidant defense, leading to oxidative stress. However, the cross-sectional design limits causal inference; these results represent associations that require validation in larger, prospective cohorts. The combined evaluation of trace elements, IMA, and TSH may serve as potential supportive biomarkers in breast cancer.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7561-7570"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148434962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined Exposure to Polystyrene Nanoplastics and Gentamicin Promotes Liver Toxicity Through Oxidative Stress, Inflammation and Apoptotic Pathways: In Vivo and In Silico Studies.","authors":"Awatef Elwej, Oumaima Essaidi, Sabrine Ben Slimen, Abir Mhadhbi, Khadija Boukholda, Rim Marrekchi, Ons Boudawara, Fatiha Chigr, Hamadi Fetoui, Yassine Chtourou","doi":"10.1007/s12011-026-05232-0","DOIUrl":"10.1007/s12011-026-05232-0","url":null,"abstract":"<p><p>Human exposure to nanoplastics and antibiotics is increasingly recognized as a major public health concern; however, the potential impacts of their combined exposure on human health remain poorly understood. In this study, we evaluated the hepatotoxic effects of polystyrene nanoplastics (PsNPs) and gentamicin (GEN), administered alone or in combination, and investigated the underlying molecular mechanisms using both in vivo and in silico approaches. Our findings demonstrated that co-exposure to PsNPs and GEN induces combined treatment response in liver by triggering oxidative stress, inflammation and apoptosis responses, associated with a dysregulation of genes related to oxidative stress (NRF2), inflammation (TNF-α, IL-1β), and apoptosis (Bax, Bcl-2, caspase-9, and caspase-3). Molecular docking analyses further supported these observations by revealing strong binding affinities of nanoplastics with key proteins involved in oxidative stress and inflammatory pathways. Our results revealed that co-exposure of adult rats to PsNPs and GEN induces an adverse effect on hepatic function. This study provides new evidence on the combined toxicity of polystyrene nanoplastics and pharmaceutical pollutants on liver function, suggesting a potential co-exposure-associated effect interaction between these contaminants.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7905-7927"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148560903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic Fluoride Exposure Disrupts Calcium-Dependent Neurodevelopmental Signalling and Brain Proteomic Architecture: Multi-Target Modulation by Naringin.","authors":"Ravindra Shantakumar Swamy, Shalini Choudhary, Abhishek Ambawatiya, Vishal Chhabra, Mayank Mishra, Md Abubakar, Smita Shenoy, Naveen Kumar, Rahul L Gajbhiye, Amita Rai, Velayutham Ravichandiran, Nitesh Kumar","doi":"10.1007/s12011-026-05250-y","DOIUrl":"10.1007/s12011-026-05250-y","url":null,"abstract":"<p><p>Long-term exposure to fluoride during early development is associated with impairments in neurobehavioral functions, oxidative stress, and molecular alterations in the developing brain. However, the mechanisms by which fluoride disrupts neurodevelopment, as well as the potential of natural flavonoids such as naringin to counteract these effects, are not well characterised. This study aimed to investigate the neurodevelopmental harms of fluoride and evaluate the multi-target neuroprotective efficacy of naringin using behavioural, biochemical, antioxidant, proteomic, and gene expression approaches in rats. Adult male and female Wistar rats and their offspring were exposed to sodium fluoride with or without naringin throughout development. Neurodevelopmental and behavioural assessments were performed along with estimation of serum and urine fluoride levels. Brain oxidative stress markers and biochemical parameters were analysed, followed by proteomic profiling (LC-MS/MS), functional enrichment analysis, and PCR validation of selected molecular markers. Fluoride exposure did not significantly affect most neonatal reflexes but produced selective impairments in specific sensorimotor parameters and pronounced behavioural deficits during post-weaning stages. Rats exposed to fluoride exhibited increased oxidative stress, altered antioxidant enzyme activities, and elevated fluoride levels in both serum and urine, highlighting systemic and neuronal toxicity. Proteomic analysis indicated a dysregulation of pathways linked to mitochondrial function, calcium signalling (including pathways associated with CALM2), synaptic plasticity, and apoptosis. PCR analysis confirmed alterations in key neuronal and stress-related genes. Co-treatment with naringin significantly alleviated behavioural impairments, restored antioxidant balance, reduced fluoride accumulation, mitigated dysregulation across multiple molecular pathways, and lessened changes in both proteomic and transcriptional markers. Fluoride exposure was associated with neurodevelopmental toxicity involving oxidative imbalance, mitochondrial dysfunction, calcium-related signalling disturbances, and alterations in synaptic pathways. Naringin attenuated many behavioural, biochemical, and molecular alterations, indicating a protective effect across multiple targets. These findings support further investigation of naringin as a potential protective compound against fluoride-induced neurodevelopmental alterations.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"8164-8185"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148696993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined Effects and Potential Mechanisms of Multiple Metals Exposure on Coronary Artery Disease in the US Population: An Integrated Epidemiological and Network Toxicology Study.","authors":"Chaoyi Bian, Chuanting Wen, Kangkang Zhong, Wanting Wang, Rui Yang, Qi Zhong","doi":"10.1007/s12011-026-05204-4","DOIUrl":"10.1007/s12011-026-05204-4","url":null,"abstract":"<p><p>Coronary artery disease (CAD) emerged as a major global health burden, but the relationship between multiple metals exposure and CAD and its toxicological mechanism were not fully understood. We analyzed the data from the National Health and Nutrition Examination Survey (NHANES) from 2001 to 2016, and evaluated the association between multiple metals exposure and CAD, including barium, cadmium, cobalt, cesium, molybdenum, lead, antimony, thallium, and uranium. Weighted Quantile Sum (WQS) regression and Quantile g-computation were constructed to explore the joint association of multiple metals exposure on CAD. Metal-related and CAD targets were obtained from Comparative Toxicogenomics Database (CTD) and Gene Expression Omnibus (GEO). Network toxicology was used to explore potential biological pathways of key metal exposure and CAD. A total of 8,962 participants was included in our study. In single metal analysis, cadmium [odds ratio (OR) and 95% confidence interval (CI) = 1.77, 95%CI:1.51-2.08], barium (OR = 0.87, 95%CI:0.78-0.97), cobalt (OR = 1.31, 95%CI:1.14-1.50) and uranium (OR = 1.20, 95%CI:1.05-1.37) were associated with CAD in total population. WQS regression and Quantile g-computation revealed that cadmium, was assigned the largest positive contribution on CAD. Network toxicology identified 44 overlapping targets potentially related to cadmium exposure and CAD. Subsequent STRING and Cytoscape analyses were used to construct a protein-protein interaction (PPI) network and highlight 13 key targets. Machine learning algorithms further identified the gene MMP9 as a key target. Our study found that multiple metals mixture exposure was associated with higher CAD prevalence and network toxicology identified MMP9 as core target. These findings should be interpreted as hypothesis-generating and further longitudinal and experimental studies are warranted to validate the role of cadmium exposure and MMP9-related pathways in CAD.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7511-7522"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148337682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}