Biological Trace Element Research最新文献

{"title":"Zinc-L-Selenomethionine Improves Growth and Hemato-Biochemical Parameters at Low but not at High Stocking Density for Nile Tilapia Males.","authors":"Carlos Henrique da Paixão Lemos, Aline Dos Anjos Santos, Crislaine Palmeira Barbosa de Oliveira, Isabelle Santos Silva, Ricardo David Couto, Luís Gustavo Tavares Braga, Luiz Vitor Oliveira Vidal, Carlos Eduardo Copatti","doi":"10.1007/s12011-025-04516-1","DOIUrl":"10.1007/s12011-025-04516-1","url":null,"abstract":"<p><p>The study aimed to examine the interaction between diets supplemented with zinc-L-selenomethionine (ZnSeMet) and two stocking densities (SD) on Nile tilapia (Oreochromis niloticus) males. Four extruded diets were formulated: 0.0, 0.5, 1.0, and 2.0 mg ZnSeMet kg^-1. Fish (58.00 g) were reared at a low SD (LSD) (15 fish tank^-1 or 4,35 kg m^-3) and a high SD (HSD) (45 fish tank^-1 or 13,05 kg m^-3). They were randomly distributed in eight treatments in triplicate in a recirculatory aquaculture system (200 L) and were fed until apparent satiation thrice daily for 65 days. The interaction between LSD and 1.0 or 2.0 mg ZnSeMet kg^-1 was better than other treatments for final weight (116.50-174.83 vs. 97.33-139.17 g), weight gain (108.83.50-116.83 vs. 39.00-80.83 g), relative weight gain (188.03-201.18 vs. 66.25-139.40%), total final length (20.12-20.63 vs. 17.33-19.00 cm), specific growth rate (1.76-1.83 vs. 0.85-1.45% day^-1) (p < 0.05). In general, the interaction between LSD and 1.0 mg ZnSeMet kg^-1 compared to several treatments, showed an increase in plasma total protein (3.23 vs. 2.67-2.78 g dL^-1) and total cholesterol (93.67 vs. 68.50-76.67 mg dL^-1) levels and a reduction in aspartate aminotransferase activity (14.83 vs. 145.67-155.17 U L^-1) (p < 0.05). The highest values for albumin (0.83 vs. 0.57 g dL^-1), glucose (56.00 vs. 45.09 mg dL^-1), and triglycerides (122.33 vs. 68.87 mg dL^-1) in the plasma, erythrocytes (2.22 vs. 2.13 × 10⁶ µL^-1), hemoglobin (9.82 vs. 9.26 g dL^-1), and hematocrit (33.09 vs. 30.21%) were observed in LSD than HSD-reared fish (p < 0.05). In conclusion, adding 1.0 mg ZnSeMet kg^-1 at LSD improved fish growth and hemato-biochemical parameters, but it was not effective at HSD.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4807-4819"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc Combined with Metformin Corrects Zinc Homeostasis and Improves Steroid Synthesis and Semen Quality in Male Type 2 Diabetic Mice by Activating PI3K/AKT/mTOR Pathway.","authors":"Huanhuan Li, Menghui Zhang, Jing Ma, Wen Li, Xuan Liu, Yuanjing Li, Jiaoying Ma, Dan Yang, Yanqing Tie, Hongzhong Bai, Shusong Wang","doi":"10.1007/s12011-025-04518-z","DOIUrl":"10.1007/s12011-025-04518-z","url":null,"abstract":"<p><p>Male infertility is a common complication of diabetes. Diabetes leads to the decrease of zinc (Zn) content, which is a necessary trace element to maintain the normal structure and function of reproductive organs and spermatogenesis. The purpose of this study was to investigate the effect of metformin combined with zinc on testis and sperm in diabetic mice. 10 of 50 male mice were randomly divided into control group (group C), and the remaining 40 mice were randomly divided into untreated diabetes group (group D), diabetes + zinc group (group Z, 10 mg/(kg • d)), diabetes + metformin group (group M, 200 mg/(kg • d)), and diabetes + zinc + metformin group (group ZM, Z 10 mg/(kg • d) + M 200 mg/(kg • d)), with 10 mice in each group. Mice fasted overnight were killed, and testes and sperm were collected for further experiments. In group D, the structure of testis was disordered, and the structure of sperm tail was destroyed and the deformity rate increased. In group D, total zinc, free zinc ions, metallothionein (MT), and metal transcription factor (MTF1) in testis were significantly decreased, while the expressions of zinc transporters ZNT7, ZIP13, and ZIP14 were significantly increased. In group D, the fluorescence intensity of free zinc in sperm tail, the expression of MT2, and MTF1 mRNA decreased significantly, while the expression of ZNT7, ZIP13, and ZIP14 mRNA increased significantly. Estrogen (E2) levels, steroid synthesis-related proteins (including CYP19A1, 3β-HSD, LHR, and STAR), and PI3K/AKT/mTOR pathway-related proteins (PI3K, p-AKT/AKT, p-mTOR/mTOR) expression were significantly decreased in group D. In addition, zinc combined with metformin activates PI3K/AKT/mTOR pathway, corrects zinc homeostasis imbalance in testis and sperm, and improves testosterone synthesis and semen quality in male type 2 diabetic mice.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4659-4670"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Levels, Exposure and Risk Assessment to Metals in Urine from Adult Population in Valencian Region (Spain).","authors":"Carmen Sáez, Alfredo Sánchez, Pablo Dualde, Miguel Ángel Aguirre, Antonio Canals, Clara Coscollà","doi":"10.1007/s12011-025-04806-8","DOIUrl":"https://doi.org/10.1007/s12011-025-04806-8","url":null,"abstract":"<p><p>Urinary levels of 19 elements (i.e., As, Ba, Be, Cd, Co, Cs, Cu, Mn, Mo, Ni, Pb, Pt, Sb, Se, Th, Tl, U, V, and Zn) in 518 adults representative of the Valencian Region in Spain were measured in the present study. Essential metals such as Cu, Zn, Mn, and Se showed 100% detection frequency (DF). Toxic metals like As and Pb were detected in 100% of participants, while Cd was found in 75%. Associations between metal levels and dietary or lifestyle factors were notable. Seafood consumption was a key contributor to elevated As, Se, and Cs levels, while smoking strongly influenced Cd, Pb, and Tl. These findings underscore the role of dietary habits in exposure to both essential and toxic metals. Hazard Quotients (HQs) were calculated for Se, Mo, Zn, Ba, Cd, and Tl using volume and creatinine-based normalizations. Essential elements posed no significant risks (HQ < 1), though Zn approached the threshold (HQ = 0.91). However toxic elements such as Cd and Tl showed minimal risk. This study highlights the need for public health strategies to reduce exposure to toxic metals such as Cd and Pb, while continuing to monitor essential metals to prevent overexposure. The results provide a valuable basis for policy making and international comparisons.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Camphor Ameliorates Cadmium-induced Memory Deficits in Rats by Downregulating NF-κB and Enhancing Acetylcholine Signaling in the Hippocampus.","authors":"Mahdieh Hamdollahi, Masoumeh Asle-Rousta, Sanaz Mahmazi","doi":"10.1007/s12011-025-04807-7","DOIUrl":"https://doi.org/10.1007/s12011-025-04807-7","url":null,"abstract":"<p><p>Cadmium (Cd) is a toxic heavy metal that can penetrate the blood-brain barrier, leading to harmful effects on the nervous system by disrupting the brain's antioxidant defense mechanisms. This research investigates how camphor, a natural compound recognized for its antioxidant effects, influences biochemical, molecular, and histological alterations in the hippocampus of rats exposed to cadmium. It also assesses its influence on the spatial memory abilities of these animals. Adult male Wistar rats were divided into four groups, each containing 10 rats: Control, Camphor, Cd, and Cd + Camphor. Cadmium chloride (5 mg/kg, orally) and camphor (10 mg/kg, intraperitoneally) were administered for 21 consecutive days. The findings revealed that treatment with camphor resulted in elevated levels of GSH, improved activities of SOD and GPx, and reduced MDA levels in the hippocampus of rats exposed to cadmium chloride. Moreover, camphor decreased the expression of pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6, while increasing the expression of IL-10. In addition, camphor diminished mRNA and protein amounts of NF-κB in the hippocampus and reduced neuronal death in the CA1 area. It decreased the expression and activity of acetylcholinesterase, enhanced the expression of BDNF and the alpha-7 nicotinic acetylcholine receptor in the hippocampus, and caused a significant improvement in spatial memory. We conclude that camphor enhanced spatial memory in rats exposed to cadmium chloride by alleviating oxidative stress, reducing neuroinflammation, and preventing neuronal loss. It plays a vital role in protecting the hippocampus by lowering NF-κB expression and boosting acetylcholine signaling.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144940999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc Attenuates Cadmium and High-Fat Diet-Induced Cardiomyopathy by Enhancing Metallothionein-Mediated Cardiac Protection.","authors":"Yaqin Liang, Yixiong Shi, Qiaoer Su, Chuangjie Gu, Ruiting Wu","doi":"10.1007/s12011-025-04803-x","DOIUrl":"https://doi.org/10.1007/s12011-025-04803-x","url":null,"abstract":"<p><p>With the worsening of environmental pollution and the rising prevalence of obesity, the combined effects of cadmium (Cd) exposure and obesity-related metabolic disorders contribute to cardiovascular injury. Therefore, it is urgent to explore their molecular mechanisms and prevention strategies. This study focused on the protective effects and mechanisms of zinc (Zn) supplementation on cardiac function in obese individuals with Cd toxicity. In present study, we established a cardiomyopathy model with dual pathological burdens of heavy Cd exposure and high fat diet induced obesity. Our results showed that Zn supplementation improved the insulin sensitivity and myocardial function. Moreover, Zn increased metallothionein levels which facilitated heavy metal chelation, reduced the harmful effects of Cd, and alleviated myocardial oxidative stress, hypertrophy, fibrosis, and apoptosis in the Cd toxicity and obesity related cardiomyopathy. These findings provide a theoretical foundation for developing nutritional intervention strategies for patients affected by environmental pollution and metabolic syndrome, while promoting the precise application of functional health products.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: Exposure to Fluoride From in Utero to Puberty Alters Gonadal Structure and Steroid Hormone Expression in Offspring Rats.","authors":"Wanpan Li, Zilong Sun, Meiyan Li, Baijuan Yue, Xuhua Zhang, Yangfei Zhao, Jundong Wang","doi":"10.1007/s12011-025-04812-w","DOIUrl":"https://doi.org/10.1007/s12011-025-04812-w","url":null,"abstract":"","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxifolin and Astilbin Protect Against Cadmium-Induced Oxidative Stress and Apoptosis in Chicken Liver Cells via Nrf2/HO-1 Signaling.","authors":"Tiezhu Chen, Tianyu Han, Yusong Miao, Liangchun Yan, Zhijun Liu, Han Dong, Tingting Cheng, Yiding Liu, ShanShan Fei, Yu Yang, Guoqiang Cheng, Guangliang Shi","doi":"10.1007/s12011-025-04802-y","DOIUrl":"https://doi.org/10.1007/s12011-025-04802-y","url":null,"abstract":"<p><p>Cadmium (Cd) is a toxic heavy metal pollutant widely present in the environment. It can accumulate in organisms through the food chain and pose a serious health threat to various organisms, including poultry. The liver is one of the main target organs for cadmium toxicity. This study aimed to explore the mechanism of cadmium exposure causing liver damage, and to explore the potential protective effects of Astilbin (ASB) and Taxifolin (TAX). Therefore, in vivo experiments with 60 one-day-old Hy-line brown male chickens were evenly divided into six groups: the CON, ASB, TAX, Cd + ASB, Cd + TAX, and Cd groups. On the 90th day, the Cd and metallothionein contents were detected in the chicken liver, and H&E and TEM were used to visualize pathological changes and ultrastructural damage of the liver tissue. Key indicators of oxidative stress, including the Nrf2 antioxidant system and apoptosis in chicken liver tissues, were also investigated. Tetramethylated Astilbin (TMA) was obtained by methylating the phenolic hydroxyl group of ASB and LMH cells, and was treated with ASB, TAX, TMA, and ML385 (a Nrf2 inhibitor). Results showed that ASB and TAX activated the expression of the Nrf2/HO-1 signaling pathway, promoted Nrf2 expression and nuclear translocation in chicken liver, and increased the activities of downstream antioxidant enzymes such as Glutathione (GSH), superoxide dismutase (SOD), and Total antioxidant capacity (T-AOC) by promoting Nrf2 translocation into the nucleus. It alleviated the increased expression of pro-apoptotic factors and the decreased expression of anti-apoptotic factors induced by Cd in the liver. This study shows that ASB and Tax can protect chicken livers from damage caused by early-life cadmium exposure.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Role of Purslane Supplementation Against Cadmium-Induced Renal and Gill Toxicity in Nile Tilapia: Insights into Antioxidant Defense and Ion Transport Regulation.","authors":"Nawal Alsubaie, Yasmina M Abd-Elhakim, Amany Abdel-Rahman Mohamed, Rowida E Ibrahim, Mohamed M M Metwally, Tarek Khamis, Badriyah S Alotaibi, Engy Mohamed Mohamed Yassin","doi":"10.1007/s12011-025-04761-4","DOIUrl":"https://doi.org/10.1007/s12011-025-04761-4","url":null,"abstract":"<p><p>Cadmium chloride (Cd) is a hazardous heavy metal known to impair antioxidant defenses and disrupt ion regulation in aquatic organisms. This study investigated the protective role of purslane (Portulaca oleracea, PUR), a medicinal plant rich in antioxidant bioactives, against Cd-induced oxidative stress and ion transporter dysfunction in Nile tilapia (Oreochromis niloticus). One hundred and eighty fish were divided into four groups (3 replicates per group, 15 fish/replicate): control, PUR (1% dietary inclusion), CdCl₂ (50 µg/L), and CdCl₂ + PUR, and exposed for 60 days. CdCl₂ exposure significantly elevated serum urea, uric acid, creatinine, glucose, and cortisol levels (by 67%, 20%, 78%, 54%, and 202%, respectively; P < 0.001), along with hypocalcemia (↓21%), hypermagnesemia (↑103%), and reduced serum iron (↓73%). Antioxidant enzyme activities in the gills were markedly suppressed (GPx (↓67%), CAT (↓67%), and SOD (↓61%)), with a depletion of GSH (↓58%) and an elevation of MDA (↑55%). The corresponding mRNA expressions of these enzymes in the kidney were downregulated (3.7-6.7-fold; P < 0.001). CdCl₂ also upregulated heat shock proteins hsp60, hsp70, and hsp90 in the  kidney (5.7-8.4-fold; P < 0.001), indicating oxidative and cellular stress. Histopathological examination revealed pronounced tissue damage in the kidney and gill structures. Ion regulation was also disrupted by CdCl₂, as evidenced by reduced gill Ca²⁺-ATPase (↓81%) and Na⁺/K⁺-ATPase (↓75%) activities, along with significant downregulation of ion transporter genes (pc, nka-α3, atp6v1a, and slc4a4; 2.3-5.2-fold decrease). Furthermore, Cd residues in the gills increased dramatically, reaching approximately 76.6-fold higher levels compared to the control group. However, dietary PUR supplementation significantly alleviated these alterations, restoring enzyme activities, reducing hsp overexpression, repairing altered architecture, reducing Cd accumulation, and normalizing ion transporter function and gene expression (P < 0.001). In conclusion, PUR demonstrates strong protective effects against CdCl₂-induced oxidative damage and ionoregulatory dysfunction in O. niloticus, likely via antioxidant-mediated and cytoprotective mechanisms. These findings support PUR (1%) as a potential dietary strategy to enhance fish resilience under heavy metal stress. Future studies should explore the long-term effects of dietary PUR, assess dose-dependent responses, and investigate molecular signaling pathways involved in its protective action.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an Ultrasound-Assisted Slurry Sampling-Based Method for Determination of Metals in Rice Samples Using FAAS.","authors":"Lays Pereira do Nascimento, Laís Silva Gomes, Cleber Galvão Novaes, Sulene Alves Araújo, Anderson Santos Souza, Geovane Silva Oliveira, Baraquizio Braga do Nascimento Junior, Marcos Almeida Bezerra","doi":"10.1007/s12011-025-04796-7","DOIUrl":"https://doi.org/10.1007/s12011-025-04796-7","url":null,"abstract":"<p><p>The present work addresses the development of a green analytical method based on slurry sampling to determine Ca, Fe, and Zn in rice. Multivariate optimization techniques (two-level fractional factorial design and Doehlert design) were used to find the experimental conditions that offer the best characteristics for the method. The slurry was made with 0.25 g of the sample in a liquid phase composed of 0.7 mol L^-1 HNO₃ and 0.31% Triton X-100 and submitted to an ultrasound bath for 7.5 min. The method has presented analytical characteristics, such as limit of quantification (4.9, 1.6, and 0.22 mg kg^-1), precision expressed as repeatability (4.4, 3.6, and 2.2%, for N = 7) respectively for Ca, Fe, and Zn, suitable for the determination of these metals in rice samples. Additionally, accuracy has been assessed by spike tests (obtaining recoveries between 90 and 108%) and comparing the values obtained by the new methodology with the standard method of acid decomposition in a digester block. The paired t-test revealed that there are no statistically significant differences (95% confidence level) between the slurry sampling method and the method adopted as standard.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesized Metal Nanoparticles as a High-Potential Tool in Bone Regenerative Medicine: A Review.","authors":"Amir Reza Sadeghifar, Alireza Farsinejad, Naghmeh Satarzadeh, Arman Shahabi, Amin Sadeghi Dousari","doi":"10.1007/s12011-025-04800-0","DOIUrl":"https://doi.org/10.1007/s12011-025-04800-0","url":null,"abstract":"<p><p>Nanoparticles are new tools used in bone tissue work that may help with bone healing and growth. Research shows that different metal nanoparticles can enhance the strength and compatibility of materials used for repairs and can also carry drugs and growth agents. Additionally, nanoparticles have been shown to positively influence mesenchymal stem cells and speed up the healing of bones. Biosynthesized nanoparticles can be a good option for clinical use in bone surgeries due to their antibacterial and anti-inflammatory properties and can also help improve the bone healing process. Nanoparticles can be made in different ways such as biological, physical, and chemical methods. Nowadays, the biological way has gained interest from researchers everywhere because it is safe, stable, and biocompatible. This review attempts to discuss studies that investigated the effects of biosynthesized metal nanoparticles in bone regenerative medicine.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}