Biological Trace Element Research最新文献

{"title":"Biosynthesis and Properties of Bismuth Nanoparticles: A Review.","authors":"Amin Sadeghi Dousari, Mojtaba Shakibaie, Mahbobeh Adeli-Sardou, Hamid Forootanfar","doi":"10.1007/s12011-024-04437-5","DOIUrl":"10.1007/s12011-024-04437-5","url":null,"abstract":"<p><p>Today, nanotechnology is becoming increasingly important among researchers around the world by helping them diagnose and treat various diseases that can threaten human life. Bismuth nanoparticles are among the numerous metal nanoparticles widely used due to their potential therapeutic applications. Variety of studies displayed the high potentials of bismuth nanoparticles in extraordinary antibacterial, antibiofilm, anticancer, and antioxidant effects, and it seems that these potentials can be used to address the challenges in the treatment of many diseases. They are among the metal nanoparticles biosynthesized by the green synthesis method in many studies. The use of green synthesis of nanoparticles has attracted the interest of many investigators because of its environmental friendliness, non-toxicity, and high stability. Microorganisms like bacteria, fungi, yeasts, actinomycetes, viruses, marine algae, and plants have been found to have the inherent potential to create metal nanoparticles intracellularly or extracellularly and are recognized as viable biofactories for the green synthesis of nanoparticles. The goal of this review article was to assess synthesized bismuth nanoparticles based on their green synthesis methods; properties in terms of shape, size, synthesis origin, and structure; and biological applications, including their antibacterial, antibiofilm, antioxidant, and cytotoxic uses.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"3949-3966"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Preventive Effect of Zinc Sulfate against Olanzapine-Induced Testicular Toxicity in Male Rats.","authors":"Hossam Ebaid, Samir A E Bashandy, Iftekhar Hassan, Jameel Al-Tamimi, Shimaa Abdallah Haredy, Tharwat Imbabi, Enayat A Omara, Yousef S Bashandy, Ezzat M Awad","doi":"10.1007/s12011-024-04442-8","DOIUrl":"10.1007/s12011-024-04442-8","url":null,"abstract":"<p><p>Male infertility is a complex and multifactorial clinical condition affecting a large population attributed to several factors, including perturbation in oxidative stress and the level of essential trace elements. Oxidative stress exerts multiple issues related to reproductive health, including male infertility, decreased sperm motility, sperm DNA damage, and an increased susceptibility to genetic disorders. Besides chemical toxins and food allergens in junk food items, many drugs can also lead to male infertility. Olanzapine (OLZ), a general antipsychotic drug, has also been reported to induce male fertility. A great deal of literature entails that supplementation of zinc can alleviate oxidative stress-related clinical complications, including male infertility. This study investigates the potential protective effects of zinc sulfate (ZnSO4) on OLZ-induced male infertility. In the present study, there were four treatment groups (n = 6): group 1 as control (without any treatment); group 2 treated with OLZ (10 mg/kg) orally daily for 6 weeks; groups 3 and 4 treated with 50 mg/kg and 100 mg/kg of ZnSO4 respectively in pre-exposed OLZ (10 mg/kg) orally daily for 6 weeks. After completion of the treatment, the biochemical analysis of serum and tissue samples demonstrated that group 2 had compromised levels of antioxidant parameters (superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH)) as well as elevated levels of stress parameters (oxidized glutathione (GSSG), malondialdehyde (MDA), and nitric oxide (NO)) as compared to the control by a significant extent (p ≤ 0.05). However, supplementation of ZnSO4 significantly corrected all these parameters in a dose-dependent way in groups 3 and 4 (p ≤ 0.05). Other health indicators, like taurine, enzyme Q10, phosphatidylcholine, ascorbic acid, and vitamin E, were also observed to improve prominently with the supplementation of ZnSO4. Intriguingly, all the fertility parameters (sperm motility, count and level of testosterone) were also found to be significantly enhanced with decreased sperm abnormalities in both the combination groups. The histological evaluation of the testis section also agreed with the biochemical analysis in this investigation. Therefore, the study strongly advocates that supplementation of Zn can ameliorate OLZ-induced male infertility to a significant extent.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"3764-3778"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12174200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Voluntary Wheel Running on Anxiety- and Depression-Like Behaviors in Fluoride-Exposed Mice.","authors":"Mengjie Qi, Yue Wu, Han Shi, Jie Liu, Run Zhu, Jixiang Wang, Amna Rafique, Bo Yang, Ruiyan Niu, Ding Zhang, Zilong Sun","doi":"10.1007/s12011-024-04433-9","DOIUrl":"10.1007/s12011-024-04433-9","url":null,"abstract":"<p><p>Fluoride, an environmental toxicant, could induce endoplasmic reticulum stress (ERS) in neuronal cells ultimately leading to apoptosis and emotional dysfunction. Meanwhile, voluntary wheel running contributes to mitigate anxiety and depression. Our investigation aimed to study the effect of voluntary wheel running on anxiety- and depression-like behaviors in fluoride-exposure mice. The results showed that exposure to 100 mg/L sodium fluoride (NaF) for 6 months can induce anxiety- and depression-like behavior in mice. Fluorosis mice subjected to voluntary wheel running have less anxiety- and depression-like behaviors. Nissl and TUNEL staining demonstrated that fluoride led to a reduced proportion of Nissl body area in the cerebral cortex and an increased apoptotic ratio of nerve cells in the cerebral cortex. In contrast, these pathologic damages were improved in voluntary wheel running mice exposed to NaF. Moreover, the expressions of mRNA in the cerebral cortex GABA, GAD65, GAD67, DR, vGLU, 5-HT1A, BDNF, NMDAR1, and Bcl₂ were downregulated and the levels of c-fos, GRP78, PERK, eIF2α, CHOP, Caspase-12, and Caspase-3 mRNA were upregulated in mice exposed to fluoride. NaF treatment had increased the PERK, ATF6, IRE1, p-eIF2α, and Caspase-3 protein levels and reduced the expressions of proteins, including GAD67, VGAT, BDNF, NMDAR1, PSD95, and SYN. By contrast, fluorosis mice subjected to voluntary wheel running enhanced the expression of GAD65, GAD67, VGAT, and neuroplasticity-related proteins in mice and inhibited the PERK-CHOP pathway. It is worth noting that the correlation between the amount of exercise and the behavioral indicators as well as neurotransmitter levels was found. In conclusion, voluntary wheel running inhibits the fluoride-induced ERS and GRP78 expression through the PERK-CHOP pathway and plays an anti-apoptotic role, ultimately ameliorating emotional dysfunction in NaF-exposed mice.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"3724-3741"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Dose of Zinc Aspartate and Zinc Citrate Attenuates Disease Activity Indices in Rheumatoid Arthritis.","authors":"Mohammad Hasan, Pooja Yadav, Mairaj Ahmed Ansari, Shakir Ali, Haider A Khan","doi":"10.1007/s12011-024-04439-3","DOIUrl":"10.1007/s12011-024-04439-3","url":null,"abstract":"<p><p>Zinc aspartate and zinc citrate have been used as zinc supplements in different health conditions. Taking into consideration their anti-inflammatory, immunomodulatory, anti-oxidant and antimicrobial properties, the present study has been designed to analyse the effect of zinc aspartate and zinc citrate treatment at therapeutic dose level on disease severity index, haematological, serological, antimicrobial and radiological markers of rheumatoid arthritis in Wistar rats. Bactericidal potential of the two organic zinc compounds was analysed in vitro in clinically isolated Escherichia coli. Arthritis was induced in male Wistar rats by intradermal injection of an emulsion containing collagen type II and Complete Freund's Adjuvant (CFA) containing 1 mg mL^-1 Mycobacterium tuberculosis H37Ra. Zinc aspartate and zinc citrate were orally administered after the onset of the disease for 4 weeks. Ameliorative effect of zinc aspartate and zinc citrate was evaluated by analysing indices of severity and disease activity markers of rheumatoid arthritis. The liver and kidney function tests were performed to evaluate any possible adverse effect of compounds. Antimicrobial activity of the zinc compounds was assessed in clinically isolated E. coli by MTT assay. Zinc aspartate and zinc citrate equivalent to a therapeutic dose of 50 mg/day of elemental zinc attenuated the clinical characteristic of rheumatoid arthritis in the animal model of arthritis, collagen-induced arthritis (CIA). Both zinc salts also exhibited antimicrobial effects against E. coli. The selected dose of zinc aspartate and zinc citrate showed no adverse effects in treated rats. This study highlights the potentiality of zinc compounds as antiarthritic agents and also point to its preventive effects on microbial growth that has been observed in rheumatoid arthritis patients due to their increased sensitivity for bacterial infection.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"3742-3753"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of Salivary Iodine Concentration in Elderly Residents of High-iodine Areas.","authors":"Mengxin Liu, Junjing Li, Hexi Zhang, Yantong Liu, Zhangzhao Wang, Zhiyuan Ren, Chunxi Wang, Qi Jin, Ying Zhang, Haohao Meng, Qiuyi Song, Wenxing Guo, Wanqi Zhang","doi":"10.1007/s12011-024-04453-5","DOIUrl":"10.1007/s12011-024-04453-5","url":null,"abstract":"<p><p>Salivary iodine is considered a potential indicator for assessing iodine nutrition status. However, there is currently a lack of research data on the characteristics of salivary iodine concentration (SIC) in the elderly population. To evaluate the characteristics of SIC and assess its efficacy in diagnosing thyroid disorders among the elderly population, a cross-sectional study was conducted in a high-iodine area. Spot urine, 24-h urine, saliva, drinking, and cooking water samples were collected from the elderly participants. Iodine concentrations were measured in all these samples. Fasting venous blood samples were obtained to assess thyroid function. The study included 1387 elderly individuals from urban (n = 707) and rural (n = 680) areas. Rural residents showed significantly higher median concentration of cooking water iodine (CWIC) and SIC compared to urban residents (P < 0.001). The median SIC in rural elderly was 2733.3 μg/L versus 860.4 μg/L in urban elderly. SIC demonstrated a significant positive correlation with age (P < 0.001). Multiple linear regression analysis revealed significant positive associations between SIC and drinking water iodine concentration (β = 0.341, 95% CI 0.312-0.370), CWIC (β = 0.393, 95% CI 0.356-0.431), thyroid dysfunction (β = 0.107, 95% CI 0.050-0.164), and goiter (β = 0.236, 95% CI 0.129-0.342). Logistic regression showed that higher SIC levels were associated with increased risk of thyroid dysfunction, with odds ratios ranging from 1.649 to 1.994 for ascending SIC categories. The elderly residents in high-iodine areas, particularly in rural settings, exhibit elevated salivary iodine concentrations that increase with age. These high-iodine levels are significantly associated with an increased risk of thyroid dysfunction.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"3658-3664"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Zinc on Blood Biochemical and mTOR Gene Expression in Rats with Polycystic Ovarian.","authors":"Faeze Fazel Torshizi, Reza Majidzadeh Heravi, Ali Javadmanesh","doi":"10.1007/s12011-024-04452-6","DOIUrl":"10.1007/s12011-024-04452-6","url":null,"abstract":"<p><p>Zinc (Zn) is a significant element of the reproductive system and is associated with several enzymes that regulate different metabolic pathways. Organic Zn can significantly affect polycystic ovarian syndrome (PCOS) pathogenesis. Insulin resistance (IR) is a common complication of PCOS. Mammalian target of rapamycin (mTOR), which controls crucial cell functions, is regulated by insulin and nutrients. It has two complexes, namely, mTORC1 and mTORC2. mTOR associates with its binding partner's regulatory associated protein of mTOR (Raptor) and rapamycin-insensitive companion of mTOR (Rictor), which form these distinct complexes, respectively, and is activated in PCOS. This research aimed to evaluate the effect of Zn on the expression of mTOR signaling genes (Raptor and Rictor) and IR in PCOS model rats. Different Zn supplements, including standard diet (SD): (control - or + , SD without supplementation), Zn25, Zn75, and Zn175 (daily given three levels of 25, 75, and 175 mg Zn methionine (ZnMet)/kg for 6 weeks, respectively), were applied to the control and PCOS groups. Fasting glucose (FG), fasting insulin (FI), IR indices, and Raptor and Rictor expression levels were measured in both groups. The results showed that PCOS induction dramatically increased FG, FI, IR indices, and mTOR-related gene expression; however, different Zn supplementation concentrations, especially at 75 mg/kg, reduced the effects of PCOS induction. Organic Zn collectively exerted positive effects on Estradiol Valerate (EV)-induced PCOS rats by reducing IR and mTOR signaling gene (i.e., Raptor and Rictor) expression. Moreover, this study revealed a correlation between Zn and IR. Therefore, Zn supplementation could be a valuable therapeutic method for treating PCOS.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"3793-3802"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Supranutritional Selenium Supplementation During Different Trimesters of Pregnancy on Humoral Immunity in Beef Cattle at Parturition.","authors":"Jean A Hall, Gerd Bobe, William R Vorachek, Joseph J Klopfenstein, Ian O Thompson, Cindy L Zurita Cruz, Brian P Dolan, Ling Jin, T Zane Davis","doi":"10.1007/s12011-024-04457-1","DOIUrl":"10.1007/s12011-024-04457-1","url":null,"abstract":"<p><p>Supranutritional Se supplementation may improve immune responses in beef cattle. Immunity is compromised in beef cattle during the periparturient period. This study aims to determine the best time during pregnancy to supplement beef cows with Se-yeast to optimize humoral immunity at parturition. Multiparous, black Angus and Angus cross cows (n = 79) were used in the study. All cows had ad libitum access to a mineral supplement containing 120 mg/kg Se (US FDA regulations) from Na selenite. In addition, all cows except controls (CTR) received Se supplementation of 105 mg Se/week from Se-yeast boluses administered once weekly during their specific treatment trimester of gestation (TR1, TR2, or TR3) for 13 weeks. This dosage was supranutritional equaling 5 × the upper range of US FDA Se administration regulations. Blood was collected at parturition from all cows. Laboratory analyses studied to assess humoral immunity included measuring IBR, BVD types 1 and 2, PI3, and BRSV serum neutralization titers post vaccination, assessing total IgM and antigen-specific IgM concentrations, and determining complement-mediated bacterial killing percentages. Statistical analyses were performed using GraphPad Prism and SAS 9.4. Supranutritional Se-yeast supplementation increased whole-blood (WB) Se concentrations regardless of trimester of supplementation (all P < 0.0001). Supplementation during TR2 and TR3 was more effective in increasing WB-Se concentrations at parturition than during TR1 or CTR (all P < 0.0001). TR2 cows had higher serum neutralization titers for BRSV compared with CRT cows (P = 0.03). Total serum IgM and Vibrio coralliilyticus-specific IgM concentrations were highly correlated (r = 0.78; P < 0.0001). Compared with CTR cows, TR1, TR2, and TR3 cows had similar total IgM concentrations (all P ≥ 0.19) and similar Vibrio coralliilyticus-specific IgM concentrations (all P ≥ 0.47). Complement-mediated bacterial killing percentages were greater in TR2 and TR3 cows (> 99.6%) compared with TR1 (93.9%) and CTR (89.3%) cows, and all Se-supplemented TR groups were greater than CTR cows (all P ≤ 0.05). The significant group differences in the complement-mediated bacterial killing assay reflected WB-Se concentrations. Supranutritional Se-yeast supplementation during TR2 and TR3 is associated with higher serum neutralization titers for some viral antigens, as well as enhanced complement-mediated bacterial killing in cows at parturition. These findings suggest that Se supplementation during later trimesters of pregnancy may help combat infectious disease challenges during the periparturient period in beef cattle.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"3709-3723"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Compositionally Different Substrates on Elemental Properties of Bay Bolete Mushrooms: Case Study of 34 Essential and Non-essential Elements from Six Areas Affected Differently by Industrial Pollution.","authors":"Alexandre V Andronikov, Irina E Andronikova, Ondrej Sebek, Eva Martinkova, Marketa Stepanova, Oksana Perehon","doi":"10.1007/s12011-024-04429-5","DOIUrl":"10.1007/s12011-024-04429-5","url":null,"abstract":"<p><p>We studied concentrations of 34 essential and non-essential elements in samples of edible Bay Bolete (Imleria badia) mushrooms added by samples of the growing substrate and bioavailable fraction. The samples were collected from six forested sites affected differently by industrial pollution and underlain by compositionally contrasting bedrock: granite, amphibolite, and peridotite. In all cases, mushrooms behaved as a bioconcentrating system for elements such as Ag, K, P, Rb, S, and Se (BCF > 1) being a bioexcluding system for the rest of the elements analyzed (BCF < 1). Most analyzed elements displayed moderate to high within-mushroom mobility being accumulated preferably in the apical parts of the mushroom's fruiting body (TF > 1). The highest mobility was demonstrated by Cd and Cu. Sodium was the only element with significantly low mobility (TF < 1), and it accumulated preferably in the stipe. Imleria badia seems to be sensitive to the accumulation of elements such as As, Cd, and Pb from the atmospheric deposits. Specific geochemistry of the growing substrate was reflected to different extend in the accumulation of elements such as Ag, Cu, Rb, S, Al, Ca, Fe, Ba, and Na in the mushroom's fruiting bodies.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"3896-3912"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12174288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Systematic Review on Fluoride Contamination in Water Resources of Iran from 2016 to 2023: Spatial Distribution and Probabilistic Risk Assessment (Monte Carlo Simulation).","authors":"Danial Nayeri, Hadis Elyasi, Ali Jafari, Mohammad Rezvani Ghalhari","doi":"10.1007/s12011-024-04422-y","DOIUrl":"10.1007/s12011-024-04422-y","url":null,"abstract":"<p><p>Because of significant variations and existence of high fluoride (F^-) in some waters, Iran has been considered for various fluoride studies in recent decades. Nevertheless, there is a no updated review on fluoride levels especially including F^- risk assessment. Thus, this systematic review is mainly aimed to evaluate the spatial distribution of F^- content in water sources of Iran using geographic information system (GIS) and conduct the health risk assessment. Besides, the Monte-Carlo Simulation technique with 10,000 iterations was applied for determination of the non-carcinogenic effects of F^- in different exposed groups (infant, children, teenagers, and adults). The results indicated that the maximum and minimum concentrations of F^- content were related to Jazmourian (Roudbar plain) (4.8 mg/L) and Sahneh (0.1 mg/L) provinces, respectively, and F^- content of more than ≅ 19% of the samples exceeds the Iranian standard value (1.5 mg/L). The results showed that the HQ of F^- in all groups were higher than 1 with the order of children > infants > teenager > adults in which children were the vulnerable group to F^- consumption in study area. Therefore, it is necessary to monitor and continuously measure water supplies for fluoride content and control measures, including removal steps, be taken for human risk reduction.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"3931-3948"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Debated Issue on Tissue Copper Levels in Colorectal Cancer Patients: A Meta-analysis and Replication Study.","authors":"Rosanna Squitti, Amit Pal, Aninda Dhar, Muhammad Aaqib Shamim, Kalyan Goswami, Anastasia De Luca, Gianluca Rizzo, Mauro Rongioletti, Vincenzo Tondolo","doi":"10.1007/s12011-024-04421-z","DOIUrl":"10.1007/s12011-024-04421-z","url":null,"abstract":"<p><p>Colorectal cancer (CRC) is a growing public health problem. Several clinical studies have shown a potentially oncogenic role of copper in CRC progression, but the reports are inconsistent. To examine published evidence on the association between tissue copper status and CRC, we carried out a systematic review and meta-analysis, searching Cochrane Library, EBSCOhost, Embase, ProQuest, PubMed/Medline, Scopus, and Web of Science for studies reporting colon tumor and matched non-cancerous tissue copper concentrations in CRC patients for articles published till June 2023. Based on a random effects model, standardized mean differences (SMD) were assessed. We also completed a replication study on 17 CRC patients that analyzed copper levels in both cancer tissue specimens and healthy mucosa dissected from the same patient. Thirteen studies investigating copper levels (including the replication study) in colorectal specimens from a pooled total of 312 CRC and 298 healthy mucosa were selected. Our meta-analysis estimated a high between-study heterogeneity (I² = 96%) and lower levels of copper in CRC tissue cancer specimens than in matched healthy mucosa: the decrease was equal to - 0.74 (95% CI, - 2.18; 0.71) but was not significant. The replication study showed a significant decrease in tissue cancer specimens. Sensitivity analyses of the meta-analysis revealed that pre-analytical methodology for tissue preparation significantly reduced the between-study heterogeneity strongly influencing copper levels (p < 0.01), indicating a copper decrease in the cytoplasmic copper pool of the tumor tissue suggesting a rapid turnover of the metal in cancer cells.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"3479-3493"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}