Biological Trace Element Research最新文献

{"title":"Egg Quality, Serum Parameters, and Bone Characteristics in Late-Phase Laying Hens Supplemented with Zinc, Manganese, and Copper with Sulfate or Amino Acids.","authors":"Arda Sözcü, Zehra Avcı Küpeli, Aydın İpek, Merve Gündüz","doi":"10.1007/s12011-025-04748-1","DOIUrl":"https://doi.org/10.1007/s12011-025-04748-1","url":null,"abstract":"<p><p>The aim of this study was to determine the effect of different zinc (Zn), manganese (Mn), and copper (Cu) combinations in different forms on egg quality, serum parameters, and bone characteristics in late-phase laying hens. A total of 600 Lohmann White layer hens at 64 weeks of age were divided into dietary groups with three repetitions (n = 40 hens/cage) in each group. The experimental groups were created as follows: control diet (Cont), supplementation of calcium carbonate (CaCO₃) as a mineral carrier (CaCO₃), sulfate form of Zn, Mn, and Cu (Sulf); Zn, Mn, and Cu-glycine chelate (Gly); Zn + Mn-methionine and Cu-lysine chelate (Met-Lys). The minerals were supplemented with an amount of 1 kg/ton of feed. The mean value of egg production was higher in both Gly (81.5%) and Met-Lys (81.4%) and the lowest in the Cont group (78.7%, P < 0.01). The eggshell thickness was found to be 0.397 mm in the Gly group and 0.399 mm in the Met-Lys group compared to the Cont (0.388 mm, P < 0.01). The lowest number of lymphocytes (34.6%) and the highest number of monocytes (26.3%) and basophils (7.0%) were observed in the Met-Lys group (P < 0.05). The plasma Zn was found to be the highest in the CaCO₃ group (176.2 µg/dL) and the lowest in the Cont (73.3 µg/dL) and Met-Lys groups (66.3 µg/dL, P < 0.001). The breaking strength and the ash content of the tibia and femur were found to be the highest in the Met-Lys group (P < 0.05). The Met-Lys supplementation could improve egg production, egg weight, and eggshell thickness, provide a hepatoprotective effects, and strengthen the effect for femur and tibia bones in laying hens during the late term of the laying period.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boric Acid and Hyperthermia Combination: A New Cancer Therapy in The Human Hepatocellular Carcinoma and Neuroblastoma?","authors":"Okan Sancer, Uğur Şahin, Vehbi Atahan Toğay, Pınar Aslan Koşar","doi":"10.1007/s12011-025-04746-3","DOIUrl":"https://doi.org/10.1007/s12011-025-04746-3","url":null,"abstract":"<p><p>Boric acid (BA) has gained attention as a potential micronutrient due to its critical cellular roles, including its apoptotic effects on various cancer cell types. Hyperthermia (HT), depending on the type of cancer, has also been shown to enhance therapeutic efficacy. This study aims to investigate the combined effects of BA and hyperthermia on HepG2 and SH-SY5Y cancer cell lines. The half-maximal inhibitory concentration (IC₅₀) of BA was first determined to assess its impact on cell viability and proliferation. IC₅₀ values of BA according to cells were found as IC_50HepG2:28.04 mM and IC_50SH-SY5Y:20.61 mM. Subsequently, DNA damage and apoptotic markers were evaluated under hyperthermic conditions at the IC₅₀ concentrations. The combination of BA and HT exhibited a significant antiproliferative effect in both cell lines, with increased expression of Caspase-9 and p53 (p < 0.05), and decreased expression of Akt-1 (p < 0.05), compared to the control group. Additionally, a significant increase in tail length (TL) was observed, indicating elevated levels of DNA damage (p < 0.05). These findings suggest that the combined application of BA and hyperthermia significantly enhances anti-proliferative, pro-apoptotic, and DNA-damaging effects in HepG2 and SH-SY5Y cells.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Iron and Its Relationship with Hematologic Parameters in Healthy Female Alpacas at Two Different Ages.","authors":"Matthias Gerhard Wagener, Max Kornblum, Frederik Kiene, Martin Ganter, Ulrike Teichmann","doi":"10.1007/s12011-025-04757-0","DOIUrl":"https://doi.org/10.1007/s12011-025-04757-0","url":null,"abstract":"<p><p>Iron is an important trace element whose deficiency is associated with anemia, which is a common condition in South American camelids. However, there are only few data available on the physiologic relationships between serum iron and hematologic findings in alpacas. In 81 laboratory diagnostic datasets from 21 clinically healthy alpacas collected over a nine-year period, we recently reported significant positive correlations between iron and hemoglobin (Hb), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and eosinophils, and significant negative correlations between iron and mean corpuscular hemoglobin concentration (MCHC), platelets, and neutrophils. For 11 of the animals, datasets were available both at the age of 1 year (young) and at an older age (4-7 years: mature). For young animals, significant positive correlations were found between iron and MCV, MCH, and eosinophils, and significant negative correlations between iron and white blood cells (WBC), red blood cells (RBC), platelets, and neutrophils. In contrast, in mature animals, significant positive correlations were found between iron and monocytes as well as eosinophils. A two-way ANOVA of these 11 animals examining the effects of age, iron, and age*iron revealed significant effects of age on eosinophils, of iron on RBC and MCH, and of age*iron on WBC, RBC, HCT, and lymphocyte-to-monocyte ratio (LMR). Our data show that both age and serum iron have an effect on hematologic parameters in alpacas. Especially in young animals there is a close association between iron and MCH and MCV and neutrophils.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adverse Impacts of Copper Oxide Nanoparticles on Rattus norvegicus: Provocation of Oxidative Stress and Depletion of Antioxidant Enzymes in Multiple Visceral Organs.","authors":"Ghulam Mustafa, Riaz Hussain, Gulnaz Afzal, Ghazala Jabeen, Abdul Ghaffar, Roshan Riaz, Mudassar Mohiuddin, Yasir Mahmood, Zeeshan Ahmad Bhutta, Tarkan Şahin, Farid Shokry Ataya","doi":"10.1007/s12011-025-04741-8","DOIUrl":"https://doi.org/10.1007/s12011-025-04741-8","url":null,"abstract":"<p><p>Brown-colored copper oxide nanoparticles (CuO NPs) were synthesized using a green synthesis method and characterized using X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FT-IR). These nanoparticles have gained significant interest during the last decade in various fields owing to their unique physical and chemical properties. However, there have been concerns regarding their toxicological potential and possible adverse health effects on living organisms and the environment. Hence, this study was conducted to unveil the mechanisms of toxicity of copper oxide nanoparticles (CuO NPs) on vital organs (brain, heart, testes, and muscles) of albino rats. After acclimatization, the rats in groups G₁-G₃ were exposed to CuO NPs via intraperitoneal route at 250, 500, and 750 mg/kg/day, respectively, for 20 days. Different behavioral changes, oxidative stress biomarkers, and histopathological alterations were recorded. Results revealed a significant dose-dependent increase in the contents of reactive oxygen species (ROS) and thiobarbituric acid reactive substances (TBARS) and a reduction of antioxidant enzymes (CAT, POD, and SOD) in different visceral organs. At the histopathological level, the brain of treated rats showed neuronal atrophy, degeneration of neurons, necrosis, and inflammatory reactions. Various sections of the testes of rats at high doses exhibited tubular degeneration, sloughing/necrosis of epithelium, and arresting of spermatogenesis, while heart sections indicated congestion, inflammatory exudate, myofibrillolysis, neutrophilic myocarditis, and coagulative necrosis. From the findings of this study, it is recorded that long-term exposure to CuO NPs induces toxicological impacts via induction of oxidative stress and disruption of physiological functions of multiple tissues of albino rats.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional Factors Affecting Magnesium Bioavailability: A Narrative Review.","authors":"Lusliany J Rondón","doi":"10.1007/s12011-025-04739-2","DOIUrl":"https://doi.org/10.1007/s12011-025-04739-2","url":null,"abstract":"<p><p>Magnesium is the second most common ion at the intracellular level (IC). Its abundance reflects its important role in the organism. Homeostasis is achieved through a delicate balance between ingestion, excretion, and reservoir, which collectively maintain physiological levels. Western dietary habits are prevalent today, often resulting in insufficient magnesium intake and compromised status. In the intestine, there are several inhibitors and enhancers of magnesium absorption. This includes physicochemical and dietary factors. This narrative review aims to evaluate the nutritional factors affecting magnesium bioavailability. These factors can be considered when planning a healthy diet and determining Dietary Reference Intakes (DRIs) for the population to prevent deficiency and non-communicable diseases. The recommendations are directed to reduce the intake of sodium, enhancing the intake of fruits and vegetables, known to be powerful alkalinizing sources and mineral-rich food.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boron's Role in B16-F10 Melanoma: Cytotoxic, Antioxidant, Apoptotic, and Anti-inflammatory Effects.","authors":"Mehmet Varol, Buğra Subaşı, Meliha Koldemir-Gündüz, Azmi Yerlikaya","doi":"10.1007/s12011-025-04750-7","DOIUrl":"https://doi.org/10.1007/s12011-025-04750-7","url":null,"abstract":"<p><p>Malignant melanoma is a fatal cancer type with a high risk of metastasis. Despite traditional treatments, the survival time of patients is usually 3-9 months. Therefore, alternative treatment strategies are needed. This in vitro study aimed to examine the potential role of boric acid in melanoma treatment by evaluating its cytotoxic, oxidative stress-related, anti-inflammatory, and apoptotic effects on the B16-F10 mouse melanoma cell line. B16-F10 cells were treated with boron at concentrations ranging from 1-100 mM for 24-48 h. Quantitative assays (n = 6 biological replicates per group) showed that boron induced dose-dependent apoptosis and significantly reduced cell viability (IC₅₀ = 16 mM). Annexin-V protein levels increased by 2.1-fold (p < 0.001) at 16 mM, while Bcl-2 protein decreased by 40% (p = 0.037). Conversely, Bcl-2 mRNA expression declined at high doses, suggesting post-transcriptional regulation. Bax and p53 gene expressions increased by 1.35-fold and 7.7-fold, respectively (both p = 0.000). Oxidative stress markers showed a 1.8-fold increase in MDA (p < 0.01) and a 25% increase in GSH (p < 0.05), indicating a redox imbalance with partial compensatory antioxidant response. The results demonstrated that boron treatment induced dose-dependent apoptosis by reducing cell viability, compared to the untreated control cells. In the cells treated with boron, while Bcl-2 gene expression was decreased, the expression of Bax, p53 gene expressions and the level of annexin-V was increased. It was also seen that TNF-α and IL-6 mRNA expression levels and IL-1β and IL-10 levels were decreased in the cells treated with boron. Following boron treatment, an increase in CAT activity, as well as higher levels of GSH and MDA, was observed. These findings suggest that boron may exert anti-melanoma effects in vitro by promoting apoptosis, modulating redox and inflammatory pathways, creating oxidative stress, and decreasing TNF-α and IL-6 levels. However, further in vivo studies are required to validate its therapeutic potential, supporting its evaluation as a promising cancer treatment agent.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chondroitin Sulfate A-Selenium Nanoparticles Activate Autophagy Through the AMPK-mTOR Pathway to Alleviate Oxidative Stress and Mitochondrial Dysfunction to Repair Kashin-Beck Disease Chondrocytes.","authors":"Huan Deng, Lichun Qiao, Yude Jiang, Abebe Feyissa Amhare, Jing Han","doi":"10.1007/s12011-025-04732-9","DOIUrl":"https://doi.org/10.1007/s12011-025-04732-9","url":null,"abstract":"<p><p>Kashin-Beck disease (KBD) is a chronic osteoarticular disease. Chondroitin sulfate A-selenium nanoparticles (CSA-SeNP), a polysaccharide-based nanoparticle, have shown promise in facilitating cartilage repair, but the mechanism remains unclear. Given our previous findings of downregulated AMPK-mTOR pathway and autophagy in KBD chondrocytes, this study explored the effects of CSA-SeNP on the AMPK-mTOR pathway and autophagy levels in KBD chondrocytes. KBD chondrocytes were treated with CSA-SeNP and AMPK inhibitors alone or in combination. We found that CSA-SeNP promoted autolysosome content and autophagic flux and upregulated the AMPK-mTOR pathway and autophagy markers, while reducing apoptosis in KBD chondrocytes. It effectively alleviated oxidative stress, as evidenced by decreased ROS level and MDA concentration, along with increased activities of antioxidant enzymes (SOD, CAT, and T-AOC). Concurrently, it also improved mitochondrial function, including elevated ATP content, enhanced SDH and ATPase activities, and restored mitochondrial membrane potential. However, co-treatment of KBD chondrocytes with CSA-SeNP and AMPK inhibitor resulted in levels of autolysosome content, autophagic flow, AMPK-mTOR pathway activity, autophagy markers, apoptosis, oxidative stress, and mitochondrial function that were intermediate between those observed with respective treatment with CSA-SeNP or AMPK inhibitor. In summary, CSA-SeNP could effectively activate AMPK-mTOR pathway to promote autophagy process, reduce oxidative stress and apoptosis, and improve mitochondrial function, thereby repairing KBD chondrocytes. This study may provide new insights into the potential of CSA-SeNP as a therapeutic agent for KBD.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cadmium-Induced Testicular and Spermatogonial Damage Ameliorated by Bryonia laciniosa Linn. Seeds in Wistar Rats: An Animal Study.","authors":"Lisong Chen, Parameswari R, Madhan Kumar P, Babujanarthanam R, Abdurahman Hajinur Hirad, Yinfang Zhou","doi":"10.1007/s12011-025-04737-4","DOIUrl":"https://doi.org/10.1007/s12011-025-04737-4","url":null,"abstract":"<p><p>Cadmium (Cd) is a ubiquitous occupational pollutant that has been extensively studied for its detrimental effects on male reproductive function, primarily through the induction of oxidative stress, which compromises both sperm quality and sperm DNA integrity. This study aimed to evaluate the protective efficacy of Bryonia laciniosa Linn., a phytochemically rich medicinal plant, against cadmium-induced reproductive toxicity in Wistar rats. Twenty-four healthy adult male Wistar rats were randomly divided into four groups (n = 6 per group): a control group, a B. laciniosa extract-treated group, a Cd-treated group, and a combination group that received both Cd and B. laciniosa extract. Rats exposed to Cd exhibited significantly reduced relative testicular weights, accompanied by impaired sperm parameters and altered spermatogonial morphology. Furthermore, Cd exposure led to significantly decreased serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone compared to the other groups. Oxidative stress biomarkers revealed that Cd exposure significantly lowered the concentrations of enzymatic antioxidants, such as superoxide dismutase (SOD) and catalase (CAT), while increasing levels of malondialdehyde (MDA) in testicular tissue. Histopathological examination of testicular cross-sections revealed considerable degeneration of spermatogenic cells, characterized by necrotic atrophy and increased eosinophilic fluid discharge in the seminiferous tubules, ultimately impairing reproductive function. Morphometric analysis was conducted using light microscopy. In contrast, treatment with B. laciniosa extract significantly reduced oxidative stress and restored enzyme levels. This restoration facilitated the regeneration of primary spermatids and spermatogonial cells in the combination treatment group, which was attributed to the extract's antioxidant-rich properties, offering protection against free radical-mediated damage and mitigating Cd-induced sperm DNA damage. The observed protective effects were primarily attributed to the synergistic antioxidant activity of B. laciniosa extract.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Role of Green-Synthesized Zinc Oxide Nanoparticles Against Prenatal Ethion Toxicity in Rats.","authors":"Nerella Venkata Pavan Kumar, V A Aneesha, Elizabeth Glanet Durom, M Remya, M Karikalan, R Raguvaran, Madhu C Lingaraju, Subhashree Parida, Avinash G Telang, Thakur Uttam Singh","doi":"10.1007/s12011-025-04743-6","DOIUrl":"https://doi.org/10.1007/s12011-025-04743-6","url":null,"abstract":"<p><p>Ethion is a class II moderately toxic organothiophosphate pesticide. The primary objective of this study was to assess the protective effect of green-synthesized zinc oxide nanoparticles (GsZnONP) against maternal and foetal toxicity induced by prenatal exposure to ethion in rats. GsZnONP was synthesized using aqueous leaf extract of Moringa oleifera and zinc acetate as a salt precursor. GsZnONP characterization revealed a size of 47.76 nm with a zeta potential of - 17.6 nm. Also, UV-visible spectrometry, FTIR, XRD, SEM, and TEM analysis revealed the formation of nanoparticles. For in vivo studies, pregnant Wistar rats were divided into four groups: control (groundnut oil), ethion (3.43 mg/kg B. Wt.), GsZnONP (10 mg/kg B. Wt.), and ethion (3.43 mg/kg B. Wt.) + GsZnONP (10 mg/kg B. Wt.) (coadministration). Oral administration was done from gestational day (GD) 6-19. Pregnant rats were sacrificed on GD 20 for maternal and foetal examination. Coadministration of GsZnONP with ethion reduced the resorptions compared to the ethion group. The hepatorenal protective effect of GsZnONP was shown by reduced levels of enzymes like AST, ALT, ALP, and creatinine in the coadministration group. It also reduced the oxidative stress induced by ethion, which is shown by the reduced MDA levels in serum, uterus, ovary, and placenta. The coadministration group also showed improvement in the histological architecture of the uterus, placenta, lungs, liver, and kidney of pregnant dams. In foetuses, coadministration improved the body weight, histological architecture of liver and kidney, and skeletal ossification. These findings suggest that GsZnONP have a protective effect over maternal and foetal toxicity induced by ethion in rats.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Marketing of Freshwater and Marine Fish Species in Alexandria City, Egypt: Human Health Risk of Specific Metals.","authors":"Khaled A Osman, Hala H Elsayed Mohamed, Maher S Salama","doi":"10.1007/s12011-025-04667-1","DOIUrl":"https://doi.org/10.1007/s12011-025-04667-1","url":null,"abstract":"","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144616116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}