Biological Trace Element Research最新文献

{"title":"Boron-Doped Nano Hydroxyapatite Grafts for Bone Regeneration in Rat Mandibular Defects.","authors":"Berat Baturay Demirkiran, Zeynep Deniz Sahin Inan, Rasim Hamutoğlu, Kerim Emre Öksüz, Zekiye Hasbek, Emine Elif Altuntaş","doi":"10.1007/s12011-024-04462-4","DOIUrl":"10.1007/s12011-024-04462-4","url":null,"abstract":"<p><p>The aim of this study was to evaluate the potential effects of boron-doped nano hydroxyapatite grafts on craniofacial bone regeneration in critical bone defects in the mandibular corpus of rats, in terms of scintigraphic and histopathological aspects. Forty Wistar albino rats, with an average weight of 200-220 g, aged 16-18 weeks, and all male, were used in the study. The rats were randomly assigned to five groups, each containing 8 rats, as follows: group C1 (no procedure applied to the mandible), group C2 (surgical defect created in the mandible but no treatment applied), group nHA (nano hydroxyapatite applied to the surgical defect area), group nHA + B1 (nano hydroxyapatite + 1% boron applied to the surgical defect area), and group nHA + B2 (nano hydroxyapatite + 2% boron applied to the surgical defect area). A standard 4 × 4 mm full-thickness transosseous bone defect was created in the mandibular corpus of all rats, except for those in group C1. The bone defect in the rats in group C2 was left to heal naturally. Nano hydroxyapatite (nHA), nano hydroxyapatite + 1% boron, and nano hydroxyapatite + 2% boron were applied to the surgical defect areas of the other three groups, respectively. Bone scintigraphy was performed on all rats on days 0 (following the surgical procedure) and 28 of the experimental period. At the end of the 28th day, the animals were sacrificed, and tissue samples were collected for histological examination. A standard grading system was used to evaluate fracture healing. When the groups were compared in terms of bone healing histopathological scores, a statistically significant difference was observed between group C1 and the other groups (p < 0.005). In the statistical evaluation made according to the histopathological mean scores, the least improvement was observed in group C2. No statistically significant difference was observed between group nHA and group nHA + B1 and group C2 and between group nHA and group nHA + B1 in terms of bone healing scores (p > 0.005). A statistically significant difference was found between group nHA + B2 and group C2 (p = 0.026). Although there was no statistically significant difference in histopathological scores, the mean score closest to group C1 was observed in group nHA + B2. A statistically significant difference was observed between the groups in the scintigraphic evaluation performed on the 28th day of the experimental procedure, and the difference was between group C1 and group nHA + B1 and between group nHA and group nHA + B1 (p = 0.004; p = 0.028, p < 0.005). In the comparison of the values obtained on days 0 and 28 within the group, a statistically significant change was observed in group nHA + B1 and group nHA + B2 (p < 0.005). When the results of the present study were evaluated, it was thought that the boron-doped nHA graft biomaterials may have positive effects on bone healing. Providing a different perspective for the development of an alternative new treatment modalit","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4139-4152"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Subacute Toxicity of Ulexite in Rats: Behavioral, Hematological, and Biochemical Insights.","authors":"Hasan Türkez, Özlem Özdemir Tozlu, Edanur Yıldız, Melik Saraçoğlu, Cem Baba, Burak Çınar, Serkan Yıldırım, Metin Kılıçlıoğlu, Kübra Çelik Topkara, Kenan Çadırcı","doi":"10.1007/s12011-024-04489-7","DOIUrl":"10.1007/s12011-024-04489-7","url":null,"abstract":"<p><p>Ulexite (UX), a naturally occurring borate mineral, has gained interest for its diverse industrial applications, yet its toxicological profile remains inadequately characterized. This study aimed to evaluate the subacute toxicity of UX in rats, focusing on behavioral, hematological, and biochemical parameters. Rats were administered UX via gavage at doses of 10, 30, and 300 mg/kg for 7 days. No mortality or significant signs of toxicity were observed, although body weight measurements indicated a notable reduction in the UX-treated groups compared to controls. Behavioral assessments demonstrated increased exploratory activity in the 10 and 300 mg/kg UX treated groups, suggesting low anxiety levels. Likewise, hematological analysis revealed that 30 and 300 mg/kg UX led a significant (P < 0.001) increase in hematocrit and a decrease in mean corpuscular hemoglobin concentration (P < 0.001), indicating potential changes in erythropoiesis. Additionally, serum biochemistry showed elevated aspartate aminotransferase (P < 0.05), lactate dehydrogenase (P < 0.001), and uric acid levels (P < 0.01), suggesting liver stress. Histopathological examinations indicated dose-dependent alterations, with mild hepatocellular degeneration and neuronal changes observed at the highest dose. Also, MN levels in the blood of rats exposed to 10 and 30 mg/kg UX showed no significant differences. These results suggest that UX is relatively safe at lower doses, though higher exposures may pose health risks. Further research is warranted to elucidate the mechanisms underlying UX-induced effects and to evaluate its safety for therapeutic and occupational applications.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4264-4272"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Zinc-Alpha-2 Glycoprotein and Zinc Levels and Their Relationship with Insulin Resistance and Biochemical Parameters in Overweight and Obese Children.","authors":"Israel Martínez-Navarro, Jenny Vilchis-Gil, Patricia Elizabeth Cossío-Torres, Héctor Hernández-Mendoza, Miguel Klünder-Klünder, Esther Layseca-Espinosa, Othir Gidalti Galicia-Cruz, María Judith Rios-Lugo","doi":"10.1007/s12011-024-04480-2","DOIUrl":"10.1007/s12011-024-04480-2","url":null,"abstract":"<p><p>The biological role of zinc-alpha 2-glycoprotein (ZAG) has been associated with lipid mobilization, although this is not entirely clear. The study's aim was to examine the serum levels of ZAG and zinc (Zn) and the Zn/ZAG in a population of children with overweight (OW) and obesity (OB), and their relationship with biochemical parameters. Our study was a cross-sectional analysis of a group of Mexican children aged 6-10 (n = 72). We analyzed anthropometric data and biochemical parameters, including fasting plasma glucose (FPG), high-density lipoprotein cholesterol (HDLc), low-density lipoprotein cholesterol (LDLc), triglycerides (TG), total cholesterol (TC), insulin, and homeostatic model assessment insulin resistance (HOMA-IR). ZAG protein levels were measured using enzyme-linked immunosorbent assay (ELISA), and serum zinc (Zn) levels were quantified using inductively coupled plasma mass spectrometry (ICP-MS). The Zn values indicate a statistically significant difference between normal weight (NW) and OW/OB children with Zn concentrations were 91 µg/dL for NW and 66 µg/dL for OW/OB children. ZAG values did not show significant differences between NW and OW/OB, and values were 2.1 mg/dL and 2.3 mg/dL, respectively. The Zn/ZAG ratio was lower in the OW/OB compared to the NW (p = 0.05). Correlations were found between FPG and Zn (p = 0.004) in NW boys, and ZAG (p = 0.046) in OW/OB boys, as well as a negative correlation between insulin and Zn in NW children of both sexes. HOMA-IR shows correlations between Zn (p = 0.008) in OW/OB boys, and ZAG (p = 0.010) in the OW/OB girls. Additionally, correlations were observed between LDLc, TG, and BMIz with Zn and ZAG in the boys. In the same way, we also found that girls with OW/OB had a Zn/ZAG ratio of - 2.32 (p = 0.043) compared to NW boys. In conclusion, our findings highlight the significant roles of Zn and ZAG in glucose and lipid metabolism. Furthermore, Zn/ZAG ratio may provide insights into nutritional deficiencies, adiposity, and metabolic health. However, further studies are necessary to validate our results.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4036-4045"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations Between Heavy Metal Exposure from Milk and Steroid Hormones in Mothers.","authors":"Zheng Wang, Caixia Liang, Li Li Shi, Cheng-Sheng Zhu, Shenghang Wang, Shoji F Nakayama, Teruhiko Kido, Xian Liang Sun, Jiancong Shan","doi":"10.1007/s12011-024-04466-0","DOIUrl":"10.1007/s12011-024-04466-0","url":null,"abstract":"<p><p>Environmental exposure to heavy metals is ubiquitous. However, its relationship with steroid hormone levels is not well understood, particularly in pregnant women. This study investigated the association between prenatal heavy metal exposure and steroid hormone levels in an e-waste disposal area in China. We analyzed the Cd, Cr, Mn, Pb, Cu, and As concentrations in 102 human milk samples collected 4 weeks after delivery. Multiple regression analysis was used to assess the associations and interactions between heavy metals and steroidal hormones. We found positive associations between Mn and estrone and estriol (estrone: β = 0.713, 95%CI = 0.046, 1.381 and estriol: β = 1.290, 95%CI = 0.494, 2.085) and between Cd and progesterone (β = 0.280; 95%CI = 0.053, 0.506). We observed negative associations between Cr and estrone and estriol (estrone: β = - 0.757, 95%CI = - 1.473, - 0.041 and estriol: β = - 1.354, 95%CI = - 2.209, - 0.499). At last, we found that Pb was negatively associated with estrone (estrone: β = - 0.537, 95%CI = - 1.053, - 0.020). Our results suggest that exposure to heavy metals may affect steroid hormone levels in mothers living in an e-waste recycling area in China.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"3989-3996"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Attenuation of Experimental Cholesterol Gallstone Formation by Manganese Chloride in Mice: Role of NF-κβ Pathways.","authors":"A T Salami, J C Orji, U Akpamu, T O Iyiola, S B Olaleye","doi":"10.1007/s12011-024-04467-z","DOIUrl":"10.1007/s12011-024-04467-z","url":null,"abstract":"<p><p>Manganese (Mn), a trace element, has been documented to exert an important role in the metabolism of cholesterol. Cholesterol gallstone (CG) pathogenesis is directly linked to biliary cholesterol imbalance which could be due to diabetes complications or mismanagement. NF-κβ pathway, an inflammatory regulator, has been implicated in metabolic disease especially in the context of diabetes and gallstone formation. However, the management of cholesterol gallstones due to diabetes with trace elements is vague. This study investigates the probable role of manganese during CG formation due to diabetes complications. Eighty female Swiss mice were grouped: I (control), II (untreated CG), III and IV (normal mice treated 0.37 mg/kg and 0.74 mg/kg Mn, respectively), V and VI (CG treated 0.37 mg/kg and 0.74 mg/kg Mn, respectively), and VII and VIII (CG treated 75 mg/7 kg and 350 mg/kg aspirin, respectively). Experimental CG was induced with cholesterol-rich diets after alloxan-induced diabetes. On sacrifice, blood collected was evaluated for complete hematological analysis and biochemistry while the excised liver was assayed for biochemical variables. Results were subjected to one-way ANOVA values were expressed as Mean ± SEM and significant at p ≤ 0.05. Manganese treatment significantly increased packed cell volume, RBC count, and hemoglobin with decreased platelet and leukocyte counts, liver enzymes (AST, ALT, and ALP), BUN, and creatinine levels in CG groups compared with untreated CG. Blood glucose, plasma low-density lipoproteins, and liver malodialdehyde levels were significantly reduced while liver nitric-oxide, sulfhydryl, and glutathione levels increased significantly in manganese-treated groups compared with untreated CG. Manganese significantly increased fecal iron contents in normal mice by the 2nd week. Hepatocytes and gallbladder histology appear normal in manganese-treated groups. Liver NF-Kβ immunoreactivity was downregulated in manganese-treated CG groups. Manganese attenuated experimental hyperglycemia-induced cholesterol gallstone by ameliorating liver oxidative stress and NF-Kβ inflammatory pathway.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4164-4182"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nickel Sulfate-Induced GSIS Injury in MIN6 Cells by Activating the JNK Pathway Through Oxidative Stress.","authors":"Bo Sun, Hui Chen","doi":"10.1007/s12011-024-04477-x","DOIUrl":"10.1007/s12011-024-04477-x","url":null,"abstract":"<p><p>Nickel has an impact on human health, especially in the context of the new energy industries. Nickel's influence on glycemia remains controversial, and the effects and mechanisms of nickel on islet function still need further exploration. MIN6 cells were treated with different concentrations of nickel sulfate (NiSO₄) (0, 75, 150, and 300 µg/mL) for different durations (0, 12, 24, and 48 h). The study measured cell cycle progression, apoptosis, reactive oxygen species (ROS) production, oxidative stress-related indexes (T-SOD, TBARS, 8-OHdG, and GSH), glucose-induced insulin secretion (GSIS), and the expression of JNK pathway-related proteins, pancreaticoduodenal homeobox-1 (PDX-1), glucose transporter 2 (GLUT2), and forkhead box protein O1 (FOXO1). NiSO₄ damaged MIN6 cells in a time- and dose-dependent manner. NiSO₄ blocked the cell cycle, induced apoptosis, and reduced insulin secretion in the GSIS experiment. NiSO₄ also induced ROS production, increased oxidative stress-related indexes (TRABS and 8-OHdG), and decreased antioxidant stress-related indexes (GSH and T-SOD). In addition, NiSO₄ activated the JNK pathway, upregulated FOXO1 protein expression, and inhibited PDX-1 and GLUT2 protein expression, affecting insulin release during GSIS. NiSO₄ inhibited the proliferation of MIN6 cells through oxidative stress, aggravated apoptosis, caused functional impairment, upregulated the expression of FOXO1 by activating the JNK pathway, inhibited the expression of PDX-1 and GLUT2 proteins, and impaired the GSIS function of islets.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4308-4317"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12174192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142806017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc Ameliorates Acrylamide-Induced Cognitive Impairment in Male Wistar Rats: Modulation of Oxidative Stress, Neuro-inflammation, and Neurotrophic Pathways.","authors":"Ayodeji Johnson Ajibare, Olabode Oluwadare Akintoye, Oluwatobiloba Adesewa Oriowo, Abraham Olufemi Asuku, Isaac Adeola Oriyomi, Abosede Mary Ayoola","doi":"10.1007/s12011-024-04490-0","DOIUrl":"10.1007/s12011-024-04490-0","url":null,"abstract":"<p><p>This study investigated the neuromodulatory potential of zinc against acrylamide-induced cognitive impairment. Acrylamide (AA), a toxic substance commonly found in certain foods such as potato, grains and coffee, is known to cause neurological damage and severe cognitive decline. Twenty (20) male Wistar rats were divided into four groups (n = 5) by random selection. All groups except Control (Group 1) which received 1 mL/kg water daily, were induced with an oral dose of 10 mg/kg of Acrylamide. Acrylamide (AA) (Group 2) was left untreated, while Low Zinc (AA + LZN-Group 3) and High zinc (AA + HZN-Group 4) were orally treated respectively with 10 mg/kg and 30 mg/kg of Zinc for 8 weeks. Zinc treatment mitigated the anxiety-like behavior and spatial and non-spatial memory deficit which are all signs of cognitive impairment observed in the AA group. Zinc reverses the significant decrease in superoxide dismutase (SOD) and catalase, significant increase in malondialdehyde (MDA) and interleukin 1β (IL-1β) caused by AA demonstrating its antioxidant and anti-inflammatory properties. Zinc also demonstrated potency in up-regulating brain-derived neurotrophic factor (BDNF) gene expression and down-regulating acetylcholinesterase (AChE) expression. Zinc treatment at both doses significantly increased the number of dentate gyrus cells. This study demonstrates the ability of zinc to mitigate the cognitive impairment secondary to acrylamide exposure.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4273-4282"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Chromium Supplementation on Serum Levels of Inflammatory Mediators: An Updated Systematic Review and Meta-analysis on Randomized Clinical Trials.","authors":"Ali Gholami, Masoudreza Sohrabi, Hamid Reza Baradaran, Mitra Hariri","doi":"10.1007/s12011-024-04486-w","DOIUrl":"10.1007/s12011-024-04486-w","url":null,"abstract":"<p><p>Chromium has been recognized for its beneficial effects on inflammation reduction; therefore, we conducted a systematic review and meta-analysis to find the effect of chromium supplementation on serum levels of C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) in subjects aged 18 years and older. Related articles were identified by searching databases such as the Cochrane Library, ClinicalTrials.gov, ISI Web of Science, Scopus, and PubMed up until Agust 2024. We computed the mean differences (MD) along with their standard deviations (SDs) to carry out the meta-analysis. Statistical heterogeneity of the intervention effects was assessed using I-squared statistics and Cochran's Q test. In total, twelve and eleven studies were included in the present systematic review and meta-analysis, respectively. The pooled results indicated that the differences in serum levels of CRP and TNF-α between chromium group and the comparison group were statistically significant (CRP: weighted mean difference (WMD) = -0.58 mg/L; 95% confidence interval (CI) = -0.95, -0.22 mg/L; P = 0.002; TNF-α: WMD = -1.22 pg/ml; 95% CI = -1.91, -0.53 pg/ml; p = 0.001). In contrast, chromium supplementation resulted in a non-significant decrease in serum levels of IL-6 (WMD = -0.63 pg/ml; 95% CI: -1.67, 0.4 pg/ml; P < 0.001). Our study supports the beneficial effect of chromium supplementation on serum concentration of CRP and TNF-α, but our results showed that chromium supplementation non-significantly reduced the serum levels of IL-6. However, it seems that chromium formulation, participants' BMI, sample size, and geographical region are strong variables that predict the effect of chromium supplementation on inflammatory mediators.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4065-4078"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Zeolite Zinc on Memory Performance and Hippocampal Cell Death in a Rat Model of Alzheimer's-like Disease Induced by Aβ_1-42.","authors":"Maryam Zaman Fashami, Aida Bajelan, Hamidreza Shakur, Fatemeh Khakpai, Fatemeh Rouhollah, Salar Vaseghi, Batool Ghorbani Yekta","doi":"10.1007/s12011-024-04474-0","DOIUrl":"10.1007/s12011-024-04474-0","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is one of the most common neurodegenerative disorders, characterized by the slow and progressive loss of brain structure and function, primarily affecting older individuals. Evidence has shown that disruption of zinc homeostasis in the brain contributes to synaptic dysfunction, as well as impairments in learning and memory. In this study, we evaluated the effect of zeolite zinc on memory performance and hippocampal cell death in a rat model of Alzheimer's disease (AD) induced by intracerebroventricular administration of Aβ_1-42. We employed the Morris water maze, shuttle box, and open field tests to assess spatial memory, passive avoidance memory, and anxiety-like behavior, respectively. P-Tau and the amyloid precursor protein (APP) expression, along with hippocampal cell death, were also evaluated. Both Aβ_1-42 and zeolite zinc were injected intracerebroventricularly. The results showed that zeolite zinc partially reversed Aβ_1-42-induced impairments in memory performance and mitigated the effects of Aβ_1-42 on locomotor activity, although it did not fully restore baseline levels. In addition, Aβ_1-42 increased the expression of APP and P-Tau, as well as the number of dead cells, whereas zeolite zinc reduced these effects. In conclusion, our findings suggest that while zeolite zinc plays a role in modulating the pathophysiology of AD, its therapeutic effects only partially reverse the progression or symptoms of AD, indicating the need for further investigation into optimal dosing or combination therapies.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4211-4223"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilizing a Combination of Supplements Comprising Boric Acid, Magnesium, Vitamin D3, and Extra Virgin Olive Oil to Improve Metabolism in Menopausal Ovariectomized Rats.","authors":"Hebatallah Husseini Atteia, Salwa Fares Ahmed, Mervat El-Sayed Askar, Amr Tawfik Sakr","doi":"10.1007/s12011-024-04476-y","DOIUrl":"10.1007/s12011-024-04476-y","url":null,"abstract":"<p><p>Metabolic syndrome during menopause can lead to diabetes, cardiovascular problems, and increased mortality rates. Hormone replacement therapy is recommended to manage climacteric complications, but it has serious adverse effects. This study, therefore, investigated the potential of supplementing some minerals, vitamins, and natural products like boric acid, magnesium, vitamin D3, and extra virgin olive oil on metabolic status of menopausal ovariectomized rats. Fourty-two female adult rats were randomly assigned to seven groups: a) Sham Control, b) Ovariectomized Control, c) Ovariectomized + Boric acid, d) Ovariectomized + Magnesium, e) Ovariectomized + Vitamin D3, f) Ovariectomized + Extra virgin olive oil, and g) Ovariectomized + Combined treatment groups. Serum inflammatory and oxidative stress markers, serum lipogram pattern, hepatic triglycerides, body weight, fasting blood glucose, serum insulin, leptin, and adiponectin, as well as hepatic insulin signaling cascade, IRS1/pAKT/GLUT4 were measured in each group to assess metabolic function. Results revealed a significant improvement in inflammation, oxidative stress, and metabolic parameters by individual and concomitant treating ovariectomized rats with boric acid, magnesium, vitamin D3, and extra virgin olive oil. Interestingly, the concurrent use of these supplements displayed a better impact than individual use, suggesting their valuable therapeutic potential for managing metabolic syndrome in menopausal women. However, the necessity of all four supplements for optimal therapeutic effects remains unsubstantiated.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4240-4255"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}