Biological Trace Element Research最新文献

{"title":"Association Between Hypochromic Microcytic Anemia in Pregnancy and Cord Blood Levels of Cadmium, Lead, Manganese, Mercury, and Selenium.","authors":"Shannon Isennock, Mohamad Elabiad","doi":"10.1007/s12011-025-04742-7","DOIUrl":"https://doi.org/10.1007/s12011-025-04742-7","url":null,"abstract":"<p><p>Iron deficiency anemia (IDA) has been associated with increased blood lead (BPb). Increased BPb in pregnancy has been associated with increased cord BPb. The association between IDA in pregnancy and cord PB levels has not been previously investigated. It is thus hypothesized that IDA in pregnancy results in increased cord BPb levels. Prospectively, cord blood, from term infants delivered in Memphis, TN, was tested for lead, manganese, mercury, and selenium. Maternal charts were retrospectively reviewed and subjects enrolled into two groups: IDA group with hemoglobin < 9 g/dL, 65 fl < MCV 75 fl, MCHC < 32 g/dL and controls with hemoglobin > 12 g/dL, 80 fl < MCV < 95 fl and MCHC > 34 g/dL. Exclusion criteria were chronic conditions, sickle cell disease, and thalassemia. Fifty-five infants were included, with 27 in the maternal anemia group and 28 in the control group. There were no significant differences between groups in maternal age, pregnancy histories, prenatal vitamin and iron intakes, or other morbidities. Pregnancies with anemia had significantly lower BPb and blood mercury (BHg) levels and significantly higher blood selenium (BSe) levels than those without anemia,0.24 (0.18,0.32) µg/L, 0.19(0.17,0.52), 184 ± 31 vs 0.33 (0.27,0.45), 0.55 (0.27,0.92), 160 ± 33, respectively. Conclusion: Pregnancies with anemia were associated with significantly higher cord BSe levels and lower BPb and BHg levels than pregnancies without anemia. Selenium consumption has been shown to lower BPb and BHg levels. This may explain our findings. Future studies are needed to investigate the role of selenium consumption as a protective agent against the transfer of heavy metals across the placenta.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc Supplementation on Growth Performance, Mineral Metabolism and Nutrient Digestibility in Lambs: a Systematic Review and Dose-response Meta-analysis.","authors":"Mahin Darabi, Fardin Hozhabri, Alihossein Piray, Saheb Foroutanifar","doi":"10.1007/s12011-025-04669-z","DOIUrl":"https://doi.org/10.1007/s12011-025-04669-z","url":null,"abstract":"<p><p>The effects of dietary zinc (Zn) supplementation on lambs fed diets without added Zn remain controversial, and the precise nature and extent of the associations between Zn administration and lamb responses are not fully understood. To address this, a comprehensive systematic review and dose-response meta-analysis was conducted to explore the effects of Zn supplementation on growth performance, mineral metabolism, and nutrient digestibility. Initially, 3169 studies were identified through comprehensive database searches using relevant keywords. Following a two-stage screening process, 30 eligible articles were selected based on predefined criteria and used in the current meta-analysis. The one-stage random-effects model was utilized in this meta-analysis, with sensitivity analyses conducted to evaluate the robustness of the derived effect sizes. The results showed nonlinear relationships between Zn supplementation and performance parameters. Optimal Zn levels were identified: approximately 28.51 mg/kg DM for maximum dry matter intake, 37.84 mg/kg DM for highest weight gain, and 42.00 mg/kg DM for best feed conversion ratio. Linear increases in fecal and urinary Zn, and a decrease in apparent Zn absorption, were observed with increasing Zn supplementation (P < 0.05). Zinc supplementation up to 53 mg/kg DM enhanced Zn retention, and up to 107 mg/kg DM, increased liver Zn concentration, with the highest concentration at 50.59 mg/kg DM. Zinc supplementation also affected copper and iron concentrations in various tissues. Specifically, it decreased pancreatic copper up to 22 mg/kg DM but increased it above 50 mg/kg DM. In kidney tissue, copper concentration increased linearly with Zn supplementation from 76 to 150 mg/kg DM, while in liver tissue, it decreased from 42 to 150 mg/kg DM. Heart tissue iron concentration increased up to 24 mg/kg DM but decreased from 41 to 150 mg/kg DM, with the highest concentration at 16.31 mg/kg DM. Zinc supplementation at 28.28 and 20.88 mg/kg DM increased total concentration of rumen volatile fatty acids and dry matter digestibility, respectively. However, final weight, carcass weight, nitrogen metabolism, Zn levels in heart, pancreas, and kidney tissues, copper levels in heart tissue, iron levels in pancreas and liver tissues, and the digestibility of most dietary fractions (organic matter, crude protein, NDF, and ADF) were not affected (P > 0.05). In summary, the findings of this meta-analysis generally align with NRC guidelines; this study underscores the necessity of precise Zn supplementation to optimize lamb productivity and mitigate potential adverse effects.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trace, Toxic (PTEs), and Rare Earth Element (REEs) Distributions in Leaves and Flowers of Elaeagnus angustifolia L.","authors":"Bouzid Nedjimi","doi":"10.1007/s12011-025-04740-9","DOIUrl":"https://doi.org/10.1007/s12011-025-04740-9","url":null,"abstract":"<p><p>Elaeagnus angustifolia L., frequently called Russian olive, is a widely used therapeutic and ornamental tree recognized for its ecological and therapeutic virtues. This work presents the first quantification and distribution patterns of major (K, Ca, and Na), trace (Ba, Cr, Co, Cs, Fe, Rb, and Z), PTEs (As, Br, and Sb), and REEs (Ce, Nd, La, Sc, Sm, and Th) in the leaves and flowers of this species using instrumental neutron activation analysis (INAA). Results disclose significant variation in element concentrations between both organs. Potassium, Ca, and Fe were dominant components in both organs. Leaves showed higher contents of Na, Ca, Cr, Cs, and Rb, whereas flowers exhibited higher contents of Co, Nd, Sb, and Th. PCA calculation displayed a clear separation between leaves and flowers based on loading scores, with leaves associating with Ca, Cr, and Rb, and flowers with Co, Nd, and Th. This investigation helps us better understand the chemical profiling and medicinal potential of E. angustifolia for continued ethnobotanical and pharmacological applications.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination and Health Risk Assessment of Heavy Metals in Some Canned Food in Tehran, Iran.","authors":"Ramin Aslani, Parisa Shavali-Gilani, Alireza Bakhtiyari, Parisa Sadighara, Najmeh Yazdanfar, Mahmood Yousefi","doi":"10.1007/s12011-025-04735-6","DOIUrl":"https://doi.org/10.1007/s12011-025-04735-6","url":null,"abstract":"<p><p>Heavy metal exposure has raised grave health concerns globally in recent decades. The objective of the present research was to examine the contents of heavy metals, including arsenic (As), cadmium (Cd), copper (Cu), iron (Fe), mercury (Hg), lead (Pb), tin (Sn), and zinc (Zn) in canned fish, canned chick peas, and canned green peas collected from Tehran, Iran, utilizing inductively coupled plasma optical emission spectroscopy (ICP-OES). Moreover, the health risks of heavy metal exposure through canned sample ingestion were evaluated utilizing Monte-Carlo simulation. The heavy metals in samples were detected in the range of 0.01 - 0.2 mg/kg for As, < LOD - 0.05 mg/kg for Cd, 0.9 - 11.8 mg/kg for Cu, 3 - 12.17 mg/kg for Fe, < LOD - 0.11 mg/kg for Hg, 0.01 - 0.9 mg/kg for Pb, 0.02 - 4.08 mg/kg for Sn, and 2.9 - 7.75 mg/kg for Zn. The mean concentration of metals in samples was within standard limits. THQ and HI values were below 1 and CR values were below 10^-6, which were acceptable. These findings suggest that the concentrations of heavy metals in canned fish, chickpeas, and green peas do not pose a health risk to Iranian consumers.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Serum Selenium with Pro-Atherogenic Adhesion Molecules, Inflammatory Cytokines and Carotid Intima-Media Thickness in Patients with Metabolic Syndrome.","authors":"Abbas Pishdadian, HamidReza Ashtari, Zohreh Sancholi, Soudabeh Hamedi-Shahraki, Farshad Amirkhizi, Aleksandra Klisic","doi":"10.1007/s12011-025-04738-3","DOIUrl":"https://doi.org/10.1007/s12011-025-04738-3","url":null,"abstract":"<p><p>Selenium (Se), an essential micronutrient with potent antioxidant properties, may modulate cardiometabolic health through multiple physiological pathways, including redox regulation and the modulation of inflammatory responses. This study aimed to investigate the relationships between serum Se levels and pro-inflammatory cytokines, adhesion molecules, and carotid intima-media thickness (CIMT) as early indicators of endothelial dysfunction in patients with metabolic syndrome (MetS). A total of 191 patients aged 20 to 50 years, diagnosed with MetS, were included in the study. In addition to glucolipid metabolic parameters, serum levels of Se, interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), E-selectin, and monocyte chemoattractant protein-1 (MCP-1) were assessed. Ultrasonography was applied to measure the CIMT among the participants. The mean serum levels of TNF-α (p-trend = 0.037), MCP-1 (p-trend = 0.005), and VCAM-1 (p-trend = 0.002) revealed decreasing trends with increasing quartiles of serum Se. Similarly, a significant decrease in the mean CIMT was observed in the participants with increasing quartiles of serum Se (p-trend < 0.001). Serum Se levels were inversely correlated with TNF-α (r = -0.24, p < 0.001), MCP-1 (r = -0.30, p < 0.001), VCAM-1 (r = -0.34, p < 0.001), and CIMT (r = -0.47, p < 0.001). In conclusion, we observed significant inverse relationships between serum Se levels and pro-atherogenic and pro-inflammatory biomarkers, as well as the mean CIMT, in patients with MetS. Se supplementation may represent a targeted nutritional strategy for reducing cardiovascular risk in patients with MetS who have low serum Se levels.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Specific Micronutrients on Semen Quality and Reproductive Outcomes in Peripubertal Sahiwal Bulls.","authors":"Vaibhav Dhaigude, Parul Chaudhary, Prasanth M Nair, Hardik Naliyapara, Mukesh Bhakat, Goutam Mondal","doi":"10.1007/s12011-025-04733-8","DOIUrl":"https://doi.org/10.1007/s12011-025-04733-8","url":null,"abstract":"<p><p>Nutritional management with micronutrient supplementation impacts sexual behaviour, health, and semen production in bulls. This study aimed to evaluate the effect of micronutrients on seminal traits, hormonal profile, age at onset of puberty, and reproductive parameters in Sahiwal bull calves. Twenty-four Sahiwal bull calves were divided into four groups of six based on age and body weight. The Control Group received a basal diet (concentrate, green, and dry roughage). Group T1 received the basal diet plus a customised mineral mixture (Zn, Cu, Mn, Cr, and Co). Group T2 received the basal diet plus a customised vitamin mixture (vitamin A and vitamin E). Group T3 received the basal diet plus customised mineral (Zn, Cu, Mn, Cr, and Co) and vitamin (vitamin A and vitamin E) mixture. Seminal traits, hormonal profiles, age at puberty, and reproductive parameters were assessed. Micronutrient supplementation significantly improved seminal characteristics and hormone status in bull calves, and the age of puberty was significantly reduced. Testosterone levels in T3 and T1 were significantly higher after 90 days. Luteinising hormone levels were significantly higher during puberty in T3. Insulin-like growth factor levels remained unaltered. Scrotal biometry indicated a linear increase (p < 0.05) in scrotal circumference, paired testicular weight, and volume. Sexual behaviour indicators did not differ significantly between treatments. The age of puberty was significantly reduced in the T3 group. Micronutrient supplementation beyond basal levels enhances testicular growth, hormone status, and seminal quality in male calves, potentially improving reproductive performance. Micronutrient supplementation beyond the basal level significantly improves testicular growth, hormone status, seminal quality, and overall performance in peripubertal Sahiwal bulls.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linalool May Exert Neuroprotective Effects Against Cadmium-Induced Hippocampal Neurodegeneration by Regulating the 4-HNE/NF-κB Signaling Pathway.","authors":"Sercan Kaya, Tuba Yalçın, Devran Ayyıldız, Buşra Akyol","doi":"10.1007/s12011-025-04734-7","DOIUrl":"https://doi.org/10.1007/s12011-025-04734-7","url":null,"abstract":"<p><p>Cadmium (Cd) is a prevalent industrial and environmental contaminant that accumulates in the tissues of animals and humans. In particular, one of the regions most affected by Cd-induced neurodegeneration is the hippocampus. Linalool (Lin) is a monoterpene capable of traversing the blood-brain barrier owing to its low molecular weight and high lipophilicity. This study sought to establish the possible neuroprotective effects of Lin against Cd-induced hippocampal neurodegeneration by focusing on the 4-hydroxynonenal (4-HNE)/Nuclear factor kappa B (NF-κB) signaling pathway. The 28 male rats used in the experiment were randomly (n = 7) distributed into four groups. The control group was not administered. The Cd group received CdCl₂ at a dose of 3 mg/kg (cumulative 21 mg/kg) for the first 7 days of the experiment. The Cd + Lin group was given 3 mg/kg CdCl₂ for the first 7 days of the experiment and 100 mg/kg Lin for 14 days throughout the experiment. The Lin group was administered Lin at a dosage of 100 mg/kg/day. After all treatments were finished, the rats' brain tissues were taken out on the 15th day of the experiment. In this study, Cd increased oxidative stress parameters and decreased antioxidant enzyme levels in hippocampus tissue. In addition, Cd caused an increase in NF-κB and pro-apoptotic Caspas3 levels, which play an important role in inflammation in the hippocampus. Lin treatment against Cd exposure showed a therapeutic effect by suppressing oxidative stress, inflammation, and apoptosis. Lin may exert a neuroprotective effect by regulating the 4-HNE/NF-κB signaling pathway in Cd exposure-induced hippocampal neurodegeneration.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of Lactobacillus curvatus Against Acute Lead and Cadmium Toxicity in Caspian Kutum (Rutilus frisii, Nordmann, 1840): Growth Performance, Chemical Composition, Hematological Characteristics, Serum Biochemical Parameters, and Immune Response.","authors":"Afshin Fahim, Masoumeh Anvari, Alireza Valipour, Mohammad Reza Safabakhsh","doi":"10.1007/s12011-025-04677-z","DOIUrl":"https://doi.org/10.1007/s12011-025-04677-z","url":null,"abstract":"<p><p>In this study, we explored the efficacy of the probiotic Lactobacillus curvatus in growth performance, chemical composition, hematological characteristics, serum biochemical parameters, and immune response of Caspian Kutum (Rutilus frisii) under HMs stress. Fish with an average initial weight of 8.05 ± 1.58 g were allocated to 40-L polyethylene tanks, with six fish per tank. These fish were divided into six treatment groups: a control group, a Lactobacillus curvatus only group (10⁸ CFU/g), a Pb exposure group (12 mg/L), a Cd exposure group (4 mg/L), a Pb + Lactobacillus curvatus group (10⁸ CFU/g with Pb), and a Cadmium + Lactobacillus curvatus group (10⁸ CFU/g with Cadmium). Each treatment was conducted in triplicate, with the fish being fed three times daily at 6% of their body weight over. The outcomes demonstrated that growth indices in fish treated with probiotic strain had a considerable enhancement compared with the control treatment (p < 0.05). The addition of 3 g of probiotics to the diet caused a remarkable growth in white blood cells, neutrophils, a reduction in lysozyme and complement, which showed a significant difference compared with the control treatment. In general, probiotic Lactobacillus curvatus have a considerable effect on the growth, blood, and immunity, and it plays an effective role in reducing injuries caused by lead and cadmium poisoning of Caspian Sea fish fingerlings (Rutilus frisii). The administration of Lactobacillus curvatus resulted in significant improvements in growth parameters compared to the control group (p < 0.05). Lactobacillus curvatus treatment Pb to a substantial increase in white blood cells and neutrophils, along with a reduction in lysozyme and complement levels, indicating an enhanced immune response. Moreover, Lactobacillus curvatus effectively mitigated the adverse effects of Pb and Cd exposure, underscoring its potential as a protective agent against heavy metal toxicity in Caspian kutum.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatics-Based Discovery of Therapeutic Targets in Cadmium-Induced Lung Adenocarcinoma: The Role of Oxyresveratrol.","authors":"Murat Isıyel, Hamid Ceylan, Yeliz Demir","doi":"10.1007/s12011-025-04730-x","DOIUrl":"https://doi.org/10.1007/s12011-025-04730-x","url":null,"abstract":"<p><p>In this study, we establish a comprehensive bioinformatic and experimental platform for revealing the molecular bases of cadmium (Cd)-induced lung adenocarcinoma (LUAD) and the therapeutic efficacy of the natural polyphenol oxyresveratrol (O-RES) against this disease. A total of 116 differentially expressed genes (DEGs) were obtained, including 30 upregulated and 86 downregulated obtained by integrating five LUAD-related transcriptomic datasets from the GEO database. Results of network analysis identified four core hub genes: upregulated (Mmp9 and Col1a1) and downregulated (Cdh5 and Pecam-1). Using the ToppFUN module, functional enrichment analysis described the identified genes in relevant oncogenic. The in vivo validation with a rat model of Cd-induced lung toxicity further confirmed that the expression levels of these hub genes were markedly changed due to the increased level of Cd, which resembled the human LUAD profile. Strikingly, co-administration of oxyresveratrol (O-RES) further completely abolished the transcriptional aberration of this signaling pathway induced by Cd, indicating that O-RES could attenuate carcinogenic signaling in Cd-induced carcinogenesis. Furthermore, molecular docking analysis revealed that O-RES exhibits strong and specific binding affinities to key LUAD-associated targets, including Mmp9, Col1a1, Cdh5, and Pecam-1, supporting its multi-targeted therapeutic potential. These findings not only underscore the potential of Mmp9, Col1a1, Cdh5, and Pecam-1 as robust biomarkers for Cd-induced LUAD but also highlight O-RES as a promising multi-targeted agent for chemoprevention and therapeutic intervention in environmentally triggered LUAD.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Association Between Potentially Toxic Elements and Chronic Kidney Disease of Unknown Etiology in Eastern Uttar Pradesh, India.","authors":"Juhi Verma, Namrata Rao, Manish Raj Kulshrestha, Shivani Singh, Shefali Singh, Vandana Tiwari, Manisha T Sharma","doi":"10.1007/s12011-025-04731-w","DOIUrl":"https://doi.org/10.1007/s12011-025-04731-w","url":null,"abstract":"<p><p>Chronic kidney disease of unknown etiology (CKDu) is highly prevalent in agricultural communities of rural areas. Environmental risks such as potentially toxic elements (PTEs) are hypothesized to be the primary contributors; however, existing research is inconsistent. This study aimed to explore the association between various PTEs (Al, Ni, As, Cd, Hg, and Pb) and their interactions in CKDu in eastern Uttar Pradesh. This was a tertiary hospital-based case-control study recruiting 211 CKDu patients along with 214 age and gender-matched controls from the same region. Urea and creatinine levels were measured using the Cobas 6000. Estimated Glomerular Filtration Rate (eGFR) was calculated based on the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) creatinine equation. PTEs were measured in the serum and urine using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Single and multi-element exposures were explored using regression model analysis. PTE levels in both serum and urine were higher in the CKDu group than in the control group and were correlated with eGFR. Multivariate regression identified that serum Cd (β =  - 0.382, p < 0.001), As (β =  - 0.332, p < 0.001), Hg (β =  - 0.250, p < 0.001), and Al (β =  - 0.222, p < 0.001) were independently associated with CKDu. From Weighted Quartile Square (WQS) regression analysis, the maximum weight in disease prevalence was Cd (41%, β = 0.78) followed by As (32%, β = 0.42) and Al (17%, β = 0.32). A Gaussian process classifier and Bayesian kernel machine regression were used to measure the overall effect of PTEs as mixtures on CKDu. Al, As, Cd, and Hg levels were independently associated with eGFR and CKDu prevalence. However, all PTEs correlated with eGFR, signifying their bioaccumulation with or without causation.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}