Biological Trace Element Research最新文献

{"title":"Boswellic Acid and Carnosine Ameliorate Vanadyl-Sulfate-Induced Renal Damage via Regulating Nrf2/HO-1 and PI3K/AKT Pathways.","authors":"Ghada M Gad, Nahla S Kotb, Khalid S Hashem","doi":"10.1007/s12011-025-04786-9","DOIUrl":"https://doi.org/10.1007/s12011-025-04786-9","url":null,"abstract":"<p><p>Nephrotoxicity is a condition caused by the negative effects of several chemotherapy treatments on the kidneys. Our target was to determine how boswellic and carnosic acids protected rats against vanadyl sulfate (VOS)-induced nephrotoxicity. A total of 30 male Wistar albino rats were used in the investigation. They were divided into sex groups (five rats in each group) as follows: Rats in the control group were given carboxymethyl cellulose (CMC) at a concentration of 0.5%. VOS group was administered a weekly intraperitoneal injection of VOS 50 mg/kg for six consecutive weeks. For 6 weeks in a row, rats in the boswellic acid group were given injections of BA every day at a dose of 100 mg/kg orally. Group receiving carnosic acid received 100 mg/kg of CA orally daily for 6 consecutive weeks. For 6 weeks, rats in the boswellic acid plus VOS group were given 50 mg/kg of VOS intraperitoneally (i.p.) once a week and 100 mg/kg of BA orally daily. For 6 weeks, the carnosic acid plus VOS group received 50 mg/kg of VOS intraperitoneally once a week and 100 mg/kg of CA orally daily. In contrast to the control group, our results demonstrated that the injection of vanadyl sulfate to the VOS group contributed a significant increase in creatinine and blood urea as well as disordered kidney oxidative antioxidant interaction. The injection of vanadyl sulfate to the VOS group produced an increase in renal MDA concentration and a decrease in renal SOD activity, GSH content, GR, and CAT activities in comparison to the control group. When compared to the control group, our study on renal mRNA expression revealed that administering vanadyl sulfate to the VOS group increases renal iNOS, PI3K, and AKT mRNA expressions and modulates the mRNA expression of renal Nrf2 and HO-1. According to the current investigation, treating rats with BA or CA can reduce their nephrotoxicity from VOS; when the defective kidney oxidative antioxidant redox levels were returned to normal, the fibrosis in the Van-treated animals decreased. These findings are consistent with interactions between the Pi3k/Akt and Nrf2/Ho-1 pathways, as one of the human body's most vital organs.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Toxic Metal Contamination and Consumer Safety of Medicinal Plants in Al-Diwaniyah Herbal Shops.","authors":"Zainab Hadi Rajih, Osamah Nawfal Oudah","doi":"10.1007/s12011-025-04804-w","DOIUrl":"https://doi.org/10.1007/s12011-025-04804-w","url":null,"abstract":"<p><p>The growing use of herbal remedies has raised concerns about toxic metal contamination. This study evaluated the levels and health risks of five metals-lead (Pb), cadmium (Cd), nickel (Ni), copper (Cu), and chromium (Cr)-in 20 commonly used medicinal herbs from four shops in Al-Diwaniyah, Iraq. Flame Atomic Absorption Spectroscopy revealed that average concentrations of most metals exceeded international safety limits, particularly for Cr, which surpassed WHO standards in all samples. Concentrations ranged from: Pb (0.38 ± 0.01 -3.77 ± 0.11 mg/kg), Cd (BLD-0.62 ± 0.03 mg/kg), Ni (0.04 ± 0.01-10.96 ± 0.76 mg/kg), Cu (5.31 ± 0.21-23.49 ± 1.40 mg/kg), and Cr (3.39 ± 0.10-7.17 ± 0.28 mg/kg). Shop 1 contributed the highest share of contamination. Health risk was assessed using EDI, THQ, HI, CR, and TCCR, based on data from 4 vendors and 280 consumers. While most EDI, THQ, and HI values were within safe thresholds, CR values for Ni and Cr showed slight elevation, and TCCR exceeded the 1 × 10⁻⁴ Limit in 50% of samples. Findings suggest that prolonged consumption of certain herbs may pose health risks, underscoring the importance of stricter quality control, source verification, and monitoring to ensure consumer safety.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-elemental Analysis of Wild Blueberry (Vaccinium Myrtillus L.) Fruits and the Soil of Its Growth from Gorski Kotar Region (Croatia).","authors":"Ivan Perković, Miljana Kurjega, Karlo Herceg, Tajana Kruhak, Darko Bakšić, Vibor Roje","doi":"10.1007/s12011-025-04777-w","DOIUrl":"10.1007/s12011-025-04777-w","url":null,"abstract":"<p><p>Wild blueberry fruit is well known for its various benefits for human health. Inter alia, blueberries are rich in various macro- and micro-elements. In this work is a multi-elemental analysis of wild blueberry fruit samples that originate from Gorski Kotar region in the Republic of Croatia. Twenty-six elements (metals, metalloids and non-metals) were quantified by means of inductively coupled plasma-optical emission spectrometry (ICP-OES) after microwave-assisted acidic digestion of the fruit samples. The results reveal that blueberry fruits from Gorski Kotar are generally safe for normal daily (100 g per day) human consumption in terms of toxic element contents. In terms of macro-nutrients (Ca, K, Mg, P and S), the investigated blueberry fruit can be a valuable source of them. With regard to content of Na, it should be noted that this element was present in low mass fractions in the tested fruit that can be considered a positive characteristic due to general excessive exposure of humans to Na, which is known to pose risks to cardio-vascular health. Also, the samples of the soil where the fruits were harvested have been tested, too. The results show that most of the samples are not overload with toxic metal(loid)s. A few exceptions have been found in the sampling points that are exposed to human activities, such as traffic.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Heavy Metals using ICP-MS in Fruits from the Developing World and their Associated Health Risks.","authors":"Tusar Kanti Roy, Sk Arafat Hossain, Md Saiful Islam, Md Fuad Hasan, Mahjabin Mazrin, Bikash Chandra Sarker, Zulhilmi Ismail, Khalid A Ibrahim, Abubakr M Idris","doi":"10.1007/s12011-025-04809-5","DOIUrl":"https://doi.org/10.1007/s12011-025-04809-5","url":null,"abstract":"<p><p>This study investigates heavy metal contamination across ten fruit varieties cultivated in Rampal and assesses the potential health risks associated with their consumption. Eight metals-Fe, Cu, Cd, Zn, Cr, Mn, Pb, and As were analyzed using an inductively coupled plasma mass spectrometer. Health risk indicators, including estimated daily intake (EDI), target hazard quotient (THQ), hazard index (HI), and cumulative carcinogenic risk (CR), were employed. All EDI values were below the maximum tolerable daily intake (MTDI), and THQ values for Fe, Cu, Cd, Zn, Cr, Mn, and Pb remained under one, indicating low individual risk. However, arsenic (As) exceeded the THQ threshold (> 1) in ripe mango, green banana, and ripe jackfruit, raising localized health concerns. Notably, the total THQ (TTHQ) surpassed one across samples, suggesting combined exposure to these metals poses cumulative health risks. The HI value (3.49 × 10¹) indicates significant potential for non-carcinogenic effects from regular consumption. CR values for As surpassed both the minimal and high-risk limits, reflecting considerable carcinogenic potential. In contrast, Pb values fell within a moderate range, indicating a comparatively lower risk of cancer. Principal component analysis (PCA) revealed two dominant patterns: PC1 grouped positively associated metals (Fe, Mn, Cu, and Cr) against negatively associated ones (Pb, Zn, Cd, As), while PC2 further differentiated these based on contrasting loadings. This suggests distinct elemental groupings based on shared spatial or source-related behaviour. The findings underscore the need for consistent monitoring and public health interventions.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AS3MT Gene Variant Shows Association with Skin Lesions in an Arsenic Exposed Population of India.","authors":"Soma Ghosh, Arijit Chakraborty, Neelotpal Das, Subhamoy Bhowmick, Kunal Kanti Majumdar, Samsiddhi Bhattacharjee, Mouli Mukherjee, Nilabja Sikdar, Sreemanta Pramanik","doi":"10.1007/s12011-025-04515-2","DOIUrl":"10.1007/s12011-025-04515-2","url":null,"abstract":"<p><p>AS3MT, GSTO2, and GSTP1 genes play important roles in the arsenic biotransformation pathway, while CYP2E1 gene has a prominent role in the metabolic activation of xenobiotics. Hence, polymorphisms of these genes might have an effect on arsenic biotransformation and could impact susceptibility to arsenical skin lesions in individuals of chronic arsenic toxicity. The present case-control study, comprising 148 subjects, attempted to evaluate genetic association between nine polymorphisms of AS3MT, GSTO2, GSTP1 and CYP2E1 genes and arsenical skin lesions in a West Bengal (WB) population. A statistically significant association was found between rs11191439 (AS3MT) and arsenical skin lesions (OR = 5.50, P-value = 0.01) using logistic regression with age and gender as covariates. Among non-genetic risk factors, age and groundwater arsenic were found to be significantly associated with skin lesions (P-value < 0.05). When haplotypes among the intragenic polymorphisms of AS3MT, CYP2E1 and GSTO2 genes were analyzed, 'ATA' and 'ACG' haplotypes of the AS3MT gene showed significant difference between the case and control. Multifactor dimensionality reduction (MDR) analysis was performed on the nine polymorphisms and groundwater and urinary arsenic for studying gene-environment interactions. Strong association was observed between groundwater arsenic and skin lesions relative to the SNPs (P-value < 10^-5). The best model with maximum testing accuracy included one SNP from the AS3MT (rs11191439) and groundwater arsenic (P-value < 0.0001). The present study documents the first report about the association of AS3MT gene variant with skin lesions in an arsenic exposed population of WB. Presumably, this is also the first study that has used MDR to investigate gene-environment interactions in arsenic-induced toxicity.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4516-4528"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium Mitigates Caerulein and LPS-induced Severe Acute Pancreatitis by Inhibiting MAPK, NF-κB, and STAT3 Signaling via the Nrf2/HO-1 Pathway.","authors":"Kamatam Swetha, Mylanayakanahosahalli Chandrashekar Indumathi, Raju Kishan, Shiva Siddappa, Chu-Huang Chen, Gopal K Marathe","doi":"10.1007/s12011-025-04531-2","DOIUrl":"10.1007/s12011-025-04531-2","url":null,"abstract":"<p><p>Severe acute pancreatitis (SAP) leads to systemic inflammation, resulting in multiorgan damage. Acute lung injury and acute respiratory distress syndrome develop in one-third of SAP patients, with a high mortality rate of 60% due to secondary complications. Patients with pancreatitis often have selenium deficiency, and selenium supplements may provide beneficial effects. This study examined the protective role of selenium in a model of SAP induced by caerulein + lipopolysaccharide (cae + LPS). Mice were administered selenium (1 mg/kg) before being challenged with caerulein (6 injections of 50 μg/kg) and LPS (10 mg/kg). At 3 h after the last caerulein injection, blood was collected for estimating pancreatic enzymes and cytokine levels, and the mice were euthanized. We performed morphological and histological studies, measured levels of protease and oxidative stress markers and conducted western blot, ELISA, and RT-qPCR analyses. We examined lung tissue histologically and estimated myeloperoxidase levels. Selenium pretreatment significantly reduced pancreatic enzyme levels such as amylase, lipase, and proteases (specifically MMPs) and reversed tissue injury in the pancreas and lungs caused by cae + LPS. In addition, selenium-treated mice showed decreased levels of inflammatory markers and chemokines. Examination of the downstream inflammatory pathways confirmed the protective effect of selenium, which mediates its anti-inflammatory and antioxidant action by inhibiting the major inflammatory signaling pathways (MAPKs, NF-κB, and STAT3) and activating the phosphorylation of Nrf2 via Nrf2/HO-1 pathways. These findings suggest that selenium may be a potential therapeutic option for treating SAP-associated secondary complications.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4728-4750"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZnO Nanoparticles Normalize Pancreas Function via the GLP-1 and Oxidative Stress Pathways in Diabetic Rats.","authors":"Fatemeh Mirzaei, Cyrus Jalili, Iraj Khodadadi, Nashmin Fayazi Hosseini, Nesrine Majdoub, Nima Naseri, Amir Mirzaei, Ebrahim Abbasi","doi":"10.1007/s12011-024-04398-9","DOIUrl":"10.1007/s12011-024-04398-9","url":null,"abstract":"<p><p>This experiment was carried out to investigate the effects of ZnO nanoparticles (ZnO NPs) on metabolic parameters, oxidative stress, apoptosis, and pathophysiological alterations of the pancreas in diabetic rats. Nanoparticle was synthesized and its characterizations were determined. We evaluate the toxicity and useful dosage of the ZnO NPs in Wistar male rats. Our experiment showed that 5 mg/kg had a useful effect and was not toxic. Hence, in the next step, the Wistar male rats were randomly divided into 3 groups as follows: (1) control rats (C); (2) diabetic rats (D), (3) diabetic rats received 5 mg/kg NP. After 4 weeks, animals were sacrificed, and blood chemical factors were measured. The oxidative stress, inflammatory, and apoptosis pathways were evaluated. Insulin and glucagon-like peptide-1 (GLP-1) mass was evaluated by immunofluorescence. The morphological changes were evaluated using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, hematoxylin and eosin (H&E), and aldehyde fuchsin. ZnO NP acts as an insulin sensitizer and normalizes blood glucose, GLP-1 levels as well as apoptosis, oxidative stress, and inflammatory pathway gene expression in diabetic rats. ZnO NP also alleviates the pathological alterations in the pancreas. This study showed that a low dose of ZnO NPs protects pancreatic β cells from oxidative stress and apoptosis. Administration of ZnO NP also normalized the pathophysiological alteration of the pancreas, thus normalizing metabolic abnormality.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4620-4634"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Analysis of Immune Characteristics of Fluorosis and Cuprotosis-Related Genes in Fluorosis Targeted Drugs.","authors":"Ruijie Ba, Bin Liu, Zichen Feng, Guoqing Wang, Shu Niu, Yan Wang, Xuecheng Jiao, Cuiping Wu, Fangfang Yu, Guoyu Zhou, Yue Ba","doi":"10.1007/s12011-025-04517-0","DOIUrl":"10.1007/s12011-025-04517-0","url":null,"abstract":"<p><p>This study aims to investigate the role of cuprotosis in fluorosis and identify potential targeted drugs for its treatment. The GSE70719 and GSE195920 datasets were merged using the inSilicoMerging package. DEGs between the exposure and control groups were found using R software. Overlapping genes of DEG and cuprotosis-related genes (CRGs) were obtained by Venn diagram and were enriched by GO and KEGG. Hub genes were identified using PPI networks and enriched by GSEA. ROC curves, the xCell algorithm, and consensus cluster analysis were utilized to evaluate diagnostic efficacy, examine immune cell infiltration, and identify cuproptosis subtypes, respectively. The GSE53937 dataset was used for external validation. The DSigDB database was used to predict small molecule drugs. Molecular docking was used to validate the relationship between small molecule drugs and hub genes. A total of 1522 DEGs (743 upregulated genes and 779 downregulated genes) and 33 overlapping genes of DEGs and CRGs were obtained. The 33 overlapping genes were enriched in ribosomal biogenesis and oxidative phosphorylation pathways. The hub genes DNTTIP2, GTPBP4, IMP4, MRPL12, MRPL13, MRPL2, MRPS2, MRPS22, NOP2, RSL1D1, and SURF6 were identified, demonstrating great diagnostic ability with AUC > 0.8. These hub genes were associated with immune response and inflammation. Two cuproptosis patterns were established based on 33 CRGs. Mepacrine was screened as a potential drug and demonstrated stability in docking with IMP4. In summary, the current study identified several CRGs that may serve as potential biomarkers for diagnosing fluorosis and are involved in fluoride-induced immune responses. Additionally, mepacrine was screened as a potential treatment for fluorosis by targeting CRGs.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4529-4542"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sub-Chronic 30 mg/kg Iron Treatment Induces Spatial Cognition Impairment and Brain Oxidative Stress in Wistar Rats.","authors":"Karima Maaroufi, Yassine Khadhraoui, Afef Moulahi, Abid Ouarghi, Kevin Poirot, Etienne Save, Hichem Sebai","doi":"10.1007/s12011-024-04511-y","DOIUrl":"10.1007/s12011-024-04511-y","url":null,"abstract":"<p><p>Iron overload has been shown to have deleterious effects in the brain through the formation of reactive oxygen species, which ultimately may contribute to neurodegenerative disorders. Accordingly, rodent studies have indicated that systemic administration of iron produces excess iron in the brain and results in behavioral and cognitive deficits. To what extent cognitive abilities are affected and which neurobiological mechanisms underlie those deficits remain to be more fully characterized. In the present study, we looked at the effects of a 30 mg/kg iron sub-chronic treatment on cognitive abilities in two hippocampal-dependent spatial tasks (place navigation, spatial/non-spatial object recognition), in relation with iron content and oxidative stress biomarkers (MDA, SOD, CAT) in the cerebellum, hippocampus, prefrontal cortex and striatum, four brain areas known to be involved in the processing of spatial information. Iron-treated rats were impaired in acquisition and retention of the platform location in the navigation task and in the spatial/non-spatial object recognition task. Iron content and MDA were found to be increased in the four brain regions of interest, but activity of the antioxidant enzymes was not modified. The results indicate that the ability of rats to process spatial information whether in place navigation or spontaneous object spatial/non-spatial recognition is disrupted following a 30 mg/kg sub-chronic treatment. The deficits are hypothesized to result from iron excess-induced oxidative stress in the network of brain areas involved in the processing of spatial information.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4635-4647"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cadmium Pollution Deteriorates the Muscle Quality of Labeo rohita by Altering Its Nutrients and Intestinal Microbiota Diversity.","authors":"Ayesha Begum, Md Golam Rabbane, Mohammad Moniruzzaman, Md Rakibul Hasan, Xuexiu Chang","doi":"10.1007/s12011-025-04524-1","DOIUrl":"10.1007/s12011-025-04524-1","url":null,"abstract":"<p><p>The detrimental effects of cadmium (Cd), a hazardous heavy metal, on fish have triggered global concerns. While the ecotoxicity of Cd on fish has been investigated, the impact of Cd on muscle quality and its correlation with the gut microbiota in fish remains scarce. To comprehensively uncover Cd effects based on preliminary muscle Cd deposition, relevant studies, and ecological Cd pollution data, we exposed Labeo rohita to Cd under concentrations of 0.00 (control), 0.05, and 0.40 mg/L for 30 days and assessed fish health, muscle quality, and intestinal bacterial diversity. We observed significant Cd bioaccumulation in the fish muscle and intestine at 0.40 mg/L treatment, adversely impacting fish health with lower growth indices, higher mortality, behavioral aberrations, and clinical anomalies. More interestingly, Cd exposure decreased muscle quality by reducing nutrient levels, including fat, protein, iron, zinc, mono and polyunsaturated fatty acids, and increasing free amino acids and saturated fatty acids. Elevated oxidative stress markers, including total superoxide dismutase (T-SOD), catalase (CAT), and hydrogen peroxide (H₂O₂), were detected in the muscles, indicating degraded quality as a result of damage to cellular structures including proteins, lipids, and DNA. Simultaneously, we found Cd exposure altered fish intestinal microbial diversity, impairing muscle nutrient assimilation, thereby influencing muscle quality. Functional predictions suggested a decrease in pathways related to fermentation and chemoheterotrophy in the exposed groups. Overall, this study highlights how Cd toxicity jeopardizes fish health and deteriorates muscle quality which needs to be addressed for human benefit.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4835-4852"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}