Biological Trace Element Research最新文献

{"title":"Geospatial Association between Keshan Disease and Selenoprotein P in Heilongjiang Province: A Bivariate Spatial Autocorrelation Analysis.","authors":"Jiacheng Li, Cheng Wang, Guijin Li, Xinshu Wang, Zhifeng Xing, Tong Wang","doi":"10.1007/s12011-025-04845-1","DOIUrl":"https://doi.org/10.1007/s12011-025-04845-1","url":null,"abstract":"<p><p>Keshan disease, a condition endemic to China, has been associated with selenium deficiency. However, spatial epidemiological research that integrates Keshan disease prevalence with selenium nutrition remains limited. This study aimed to explore the bivariate spatial autocorrelation between Keshan disease prevalence and serum selenoprotein P levels in Heilongjiang Province, in order to provide evidence for disease prevention and control. Prevalence data for all forms of Keshan disease were obtained through a national surveillance project and analyzed using spatial empirical Bayesian smoothing. Serum selenoprotein P levels were measured using enzyme-linked immunosorbent assay. Bivariate spatial autocorrelation analyses were conducted to assess the relationship between selenoprotein P levels and the prevalence of overall Keshan disease, chronic Keshan disease, and latent Keshan disease. Local spatial autocorrelation analysis of selenoprotein P levels revealed three low-low clusters, one low-high cluster, five high-low clusters, and one high-high cluster. No bivariate global spatial autocorrelation was observed between selenoprotein P levels and the prevalence of overall Keshan disease, chronic Keshan disease, or latent Keshan disease. In total, eight local clusters were identified, including three high-low and five low-high clusters. Nine clusters were identified as key regions for Keshan disease. The risk of Keshan disease among low-selenium nutrition groups was concentrated in three clusters. Residents living in or adjacent to these regions should be prioritized for prevention and control interventions.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heavy Metal Analysis of Commercial Rice Grains from Varanasi City of India: Insight into Contamination Level, Daily Intake and Associated Health Risks.","authors":"Priyanka Singh, Rajesh Kumar Sharma, Prince Kumar Singh","doi":"10.1007/s12011-025-04827-3","DOIUrl":"https://doi.org/10.1007/s12011-025-04827-3","url":null,"abstract":"<p><p>Rice (Oryza sativa L.) is a staple food in Many Asian countries and plays a vital role in global food security. Heavy metal contamination of the rice grains is a global concern, yet data on contamination level and associated risks in Northern India remain Limited. Thus, the present study quantified concentrations of Cd, Cr, Pb, As, Ni, Cu, and Zn in 19 rice grain varieties consumed by urban population of India using an Atomic Absorption Spectrophotometer. Hazardous risks of consuming rice grains were assessed through computing estimated daily intake (EDI), target hazard quotient (THQ), and target cancer risk (TCR). Results showed that Pb, As, and Ni levels in rice grains were below the detection thresholds (0.2, 0.2, and 0.04 µg g⁻¹ dw, respectively). However, concentrations of Cd, Cr, Cu and Zn ranged between 0.25-1.3, 0.25-4.1, 1.8-10.5 and 19.5-36.8 µg g⁻¹ dw, respectively. Except Cd, all the heavy metals were below the international and Indian permissible standards. Cd and Cr were detected in six rice varieties only (Jeera 32, Chintu, Cuttack, Silky, Kinki and Gawahi). EDI values via rice consumption were found as Zn > Cu > Cr > Cd for both children and adults. THQ values for Cd, Cr, and Cu exceeds a unit in Sonanchal, Kalanamak, and Moti under high exposure frequency. TCR further revealed that Cd and Cr in rice varieties posed carcinogenic risks to local residents. Ensuring food and public health safety adheres to strict monitoring and regulations of heavy metal contamination in rice grains.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Borax Triggers Ferroptosis by Modulating the TfR1/GPX4/ACSL4 Pathway in Prostate Cancer Cells with Differential Androgen Sensitivity.","authors":"Ekrem Basaran, Ceyhan Hacioglu, Dursun Baba, Arda Taşkın Taşkıran, Ahmet Yıldırım Balık","doi":"10.1007/s12011-025-04843-3","DOIUrl":"https://doi.org/10.1007/s12011-025-04843-3","url":null,"abstract":"<p><p>Prostate carcinoma remains a predominant contributor to cancer-associated morbidity in males, with rising prevalence and therapeutic resistance highlighting unmet clinical needs. Ferroptosis-a non-apoptotic cell death mechanism mediated through iron-catalyzed phospholipid peroxidation-has emerged as a promising strategy to circumvent drug-resistant malignancies. We investigated the mechanistic interplay between transferrin receptor 1 (TfR1) and borax-mediated ferroptotic activation in androgen-responsive and androgen-independent prostate adenocarcinoma models. Cytotoxic effects of borax on LNCaP and DU-145 cells were assessed using CCK-8 and BrdU assays across 2.5 μM to 320 μM concentrations over 24-72 h. Expression and levels of TfR1, GPX4, ACSL4, GSH, MDA, total ROS, and intracellular Fe²⁺ were evaluated through ELISA, Western blotting, and RT-PCR. Ferroptosis specificity was confirmed using ferrostatin-1 inhibition studies and exclusion of apoptosis/necroptosis/autophagy by pharmacological inhibitors. Nuclear alterations and superoxide anion production were examined using DAPI staining. IC₅₀ values were 138 μM (95% CI: 115-153 μM) for LNCaP cells and 92.1 μM (95% CI: 83.4-102 μM) for DU-145 cells, with DU-145 demonstrating higher sensitivity. Borax exposure in DU-145 cells decreased GSH and GPX4 levels while increasing MDA, ROS, Fe²⁺, ACSL4, and TfR1 expression. Ferrostatin-1 pretreatment effectively attenuated these effects, confirming ferroptosis-dependent mechanisms. Nuclear abnormalities and elevated superoxide production were observed. These findings demonstrate that borax induces cytotoxicity in prostate cancer cells through ferroptosis via TfR1/GPX4/ACSL4 cascade modulation, suggesting a potential role for TfR1 in governing ferroptotic vulnerability. While high concentrations limit immediate clinical application, these results establish mechanistic foundations for ferroptosis-targeted therapy development in prostate cancer.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial Synthesis of Nano Trace Minerals: A Comprehensive Review on Synthesis, Characterization, Biological Effects and Applications in Farm Animals.","authors":"Muniswamy Shobha, Duraisamy Rajendran, Gopalakrishnan Menon, Sriramulu Mohana, Marappan Gopi, Artabandhu Sahoo","doi":"10.1007/s12011-025-04836-2","DOIUrl":"https://doi.org/10.1007/s12011-025-04836-2","url":null,"abstract":"<p><p>In recent years, nano trace minerals have acquired considerable recognition in the agricultural sector, particularly as supplements in farm animals, owing to unique physicochemical characteristics like enhanced surface area, absorbability and bioavailability. These characteristics are largely assessed by the shape and size of the nanoparticles, making them more effective than bulk particles in various biological applications. Traditional methods of synthesizing nano minerals, using physical and chemical approaches, can be expensive, energy-consuming and potentially harmful to the environment. In contrast, microbial synthesis of nano trace minerals offers a promising, economical and environmentally sustainable alternative. This approach leverages the natural metabolic pathways of microorganisms that can produce nanoparticles either via intracellular or extracellular routes. Compared to plant extract-based nanoparticle synthesis, microbial synthesis has several advantages, including better control over particle size and enhanced stability. Additionally, microbial synthesis methods are inherently more industrially feasible and economically viable, making them an ideal option for large-scale applications. Nano trace minerals have shown improved bioavailability and effectiveness in farm animals, particularly in enhancing growth, immune function and disease resistance. These benefits have gained interest in the use of nano trace minerals as feed additives, promoting better animal health and productivity. This review discusses the microbes-mediated synthesis of nano trace minerals, their characterization, biological effects and potential applications, highlighting their pros and cons over traditional mineral supplementations in farm animal nutrition.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arsenic Poisoning in Bihar: A Comparative Health Risk Assessment Through Water and Food Sources.","authors":"Nirmal Kumar Chayal, Arun Kumar, Kumar Sambhav Verma, Radhika Agarwal, Kanhaiya Kumar, Shivam Kumar, Dhruv Kumar, Tejasvi Pandey, Mohammad Ali, Abhinav Srivastava, Akhouri Bishwapriya, Ashok Sharma, Manisha Singh, Ashok Kumar Ghosh","doi":"10.1007/s12011-025-04824-6","DOIUrl":"https://doi.org/10.1007/s12011-025-04824-6","url":null,"abstract":"<p><p>Arsenic poisoning from groundwater has caused serious health hazards to the exposed population globally. The human body is contaminated by arsenic through drinking water and food, causing skin manifestations, neurological disorders, gastrointestinal disorders, cancer, etc. In the present study, a total of n = 286 household handpump water samples and food consumed (wheat and rice) by the studied subjects and each household member's biological samples (hair and nail) were collected for arsenic quantification. The hazard quotient (HQ) and cancer risk (CR) study was evaluated in the exposed studied population from the consumed wheat and rice. Arsenic content in 14% of water samples, 44% of wheat samples, and 3% of rice samples have exceeded their respective permissible limits of 10 μg/L, 100 μg/kg, and 200 μg/kg. In the studied subjects, 69% had arsenic contamination in their hair samples more than the permissible limit (200 μg/kg), while 11% had arsenic contamination in their nail samples more than the permissible limit (500 μg/kg). Arsenic contamination in the participants' household water, hair, and nail samples was positively correlated with one another by a correlation coefficient study. The food samples showed a significant correlation between the subjects' hair and nail samples and rice and wheat. In the six districts of Bihar, the geospatial analysis of all the variables revealed a considerable level of arsenic contamination. The health risk assessment also showed a high risk of arsenic contamination through wheat rather than rice. The risk assessment revealed elevated hazard quotient (HQ) and cancer risk (CR) in the six districts of Bihar's arsenic-exposed population. The present study thus concludes that arsenic contamination in water and food grains is causing health hazards in the studied exposed population of Bihar. Therefore, there is an urgent need to initiate an effective mitigation plan to safeguard the health of the exposed population.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inflammation Mediates the Associations Between Selenium Status and Glucose Metabolism in Chinese Females Over 45 Years Old: a National Cross-Sectional Study.","authors":"Xiyao Liu, Yang Cao, Jingxin Yang, Jie Feng, Wenxuan Wu, Huidi Zhang, Jiaxi Lu, Yuting Li, Lichen Yang","doi":"10.1007/s12011-025-04814-8","DOIUrl":"https://doi.org/10.1007/s12011-025-04814-8","url":null,"abstract":"<p><p>Selenium (Se) status has been reported to be associated with the risk of impaired glucose metabolism, Yet the relationship remains inconclusive, and the potential mechanisms are unclear. The present study aimed to examine the associations of selenium biomarkers with type 2 diabetes (T2D) risk and glucose metabolism among females over 45 years and to explore the mediation effects of inflammation and oxidative stress. The cross-sectional study was conducted based on China Adult Chronic Disease and Nutrition Surveillance (2015-2017), including 1,430 participants. Plasma concentrations of selenium biomarkers [Se, selenoprotein P (SeP), glutathione peroxidase 3 (GPx3)] and glucose metabolism indicators [fasting blood glucose (FBG), fasting plasma insulin (FPI), glycated hemoglobin (HbA1c), homeostasis model assessment of insulin resistance (HOMA-IR)] were measured. Representative inflammatory and oxidative stress indicators were selected, and inflammatory score and oxy-score were further calculated to integrate the effects of single indicators. A high level of SeP was associated with a higher risk of T2D (OR = 1.48, 95%CI: 1.25-1.76, P-trend < 0.05), and with higher FBG, FPI, HbA1c and HOMA-IR (P < 0.05). Plasma Se was positively associated with FBG, FPI and HOMA-IR (P < 0.05). GPx3 only has a positive association with FBG (P < 0.05). Inflammatory score and interleukin-6 (IL-6) may partially mediate the associations between selenium biomarkers and glucose metabolism (P-mediation < 0.05). Excessive Se exposure is associated with an elevated risk of glucose metabolism abnormality, especially while SeP was used to evaluate the selenium status. Inflammation was found to partially explain the above associations, indicating further longitudinal research is needed.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seminal Zinc Levels and Their Nonlinear Relationship with Sperm Concentration: A Chinese Population-Based Study.","authors":"Jiaoying Ma, Yingbo Nan, Huanhuan Li, Peipei Deng, Shusong Wang, Yanqing Tie, Jing Ma","doi":"10.1007/s12011-025-04825-5","DOIUrl":"https://doi.org/10.1007/s12011-025-04825-5","url":null,"abstract":"<p><p>Zinc is essential for testicular development, spermatogenesis, sperm protection, and male fertility maintenance. However, existing research on the relationship between zinc and sperm quality parameters shows inconsistent results. This study aimed to evaluate seminal plasma zinc levels and analyze their correlation with sperm parameters. We collected 791 male semen samples, measuring seminal plasma zinc levels via spectrophotometry and assessing the linear relationship between zinc levels and sperm parameters using RCS curves and threshold effect analysis. The results demonstrated an inverted U-shaped association between seminal plasma zinc concentration and both sperm concentration and total sperm count (p for nonlinear < 0.001). When the concentration of zinc in seminal plasma is < 156.54 mg/L, sperm concentration increases with the increase of zinc concentration in seminal plasma (β = 0.19, 95% CI: 0.12-0.26, p < 0.001), but when it is > 156.54 mg/L, although there is no statistically significant difference (β = -0.09, 95% CI: -0.20-0.02, P =0.097), sperm concentration shows a downward trend (β = -0.09, 95% CI: -0.20-0.02, p = 0.097). When the concentration of zinc in seminal plasma reaches 158.13 mg/L, the total sperm count increases significantly (β = 0.71, 95% CI: 0.45-0.96, p < 0.001), but it decreases when it exceeds this level (β = -0.31, 95% CI: -0.73 - 0.11, p = 0.152). Total zinc content demonstrated a positive linear correlation with both sperm concentration and total sperm count (p < 0.001). The findings revealed a nonlinear association between seminal plasma zinc levels and both sperm concentration and total sperm count. Total zinc content exhibited a linear relationship with these seminal parameters. These results highlight the critical need to establish a safe supplementation threshold for seminal plasma zinc.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship Between the Single Nucleotide Polymorphism A35C in the Cu/Zn-Superoxide Dismutase-1 Gene and Glycemic Control in Individuals with Type 2 Diabetes Mellitus.","authors":"Gabrielli Barbosa de Carvalho, Paula Nascimento Brandão-Lima, Ramara Kadija Fonseca Santos, Vivianne de Sousa Rocha, Alan Santos Oliveira, Beatriz da Cruz Santos, Cynthia Batista Santos, Aline Rocha Reis, Carla Soraya Costa Maia, Ana Mara de Oliveira E Silva, Liliane Viana Pires","doi":"10.1007/s12011-025-04555-8","DOIUrl":"10.1007/s12011-025-04555-8","url":null,"abstract":"<p><p>Hyperglycemia in type 2 diabetes mellitus (T2DM) increases oxidative stress. Furthermore, the presence of the single nucleotide polymorphism A35C (SNP A35C) in Cu/Zn-superoxide dismutase1 (SOD1) gene is closely related to this increase in oxidative stress and the development and progression of T2DM and its complications. This study aimed to evaluate the association between SNP A35C (rs2234694) genotypes and glycemic control in T2DM individuals. A total of 110 individuals were evaluated for anthropometric parameters, body composition, glycemic metabolism markers (fasting serum glucose, %Hb_A1c, insulin, C-peptide, and HOMA-IR, -%B, -%S), and SOD activity. Individuals were grouped according to SNP A35C genotypes. Variables of interest were assessed according to groups. The T-test for independent samples or the Mann-Whitney U test was used to analyze the differences in continuous variables between groups, and the chi-square test was performed for categorical variables. A binary logistic regression model was constructed, with p < 0.05 considered significant. Overweight was found in 81.8% of individuals with T2DM. Individuals with the AC genotype for SNP A35C had higher levels of fasting serum glucose (p = 0.018) and lower values of HOMA-%B (p = 0.044). The presence of the variant allele was positively associated with higher values of fasting serum glucose (OR: 11.340; 95%IC 1.173-109.649; p = 0.036) and HOMA-IR (OR: 9.987; 95%IC 1.127-88.506; p = 0.039). Individuals with the AC genotype of SNP A35C had poorer glycemic control than individuals with the AA genotype, and the presence of the variant allele was associated with poor glycemic control in T2DM individuals.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"5034-5040"},"PeriodicalIF":3.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Seasonality on the Contamination of Mussels (Perna perna) and Swimming Crabs (Achelous spinimanus) by Essential and Toxic Metals and the Health Risks of the Consumer Population on the Southeast Coast of Brazil.","authors":"Alexandre Mendes Ramos-Filho, Paloma de Almeida Rodrigues, Adriano Teixeira de Oliveira, Júlia Vianna da Anunciação de Pinho, Bernardo Ferreira Braz, Ricardo Erthal Santelli, Carlos Adam Conte-Junior","doi":"10.1007/s12011-025-04558-5","DOIUrl":"10.1007/s12011-025-04558-5","url":null,"abstract":"<p><p>The marine environment is severely contaminated by anthropogenic activities. Among the pollutants are metals such as mercury (Hg), chromium (Cr), and zinc (Zn), elements commonly found in industrial effluents from steel mills. This study aims to quantify the concentrations of Hg, Cr, and Zn in seafood samples (crab and mussels) and conduct a consumption risk analysis, considering the seasonality and sexual dimorphism of the animals. The samples were collected from traditional fisheries in Itaipu, Niterói, Rio de Janeiro, Brazil. Muscle samples were removed from the animals, which served as the basis for determining Hg, Cr, and Zn levels. Consumption risk assessment was also conducted following the Estimated Monthly Intake and Hazard Quotient (HQ). The quantification results were below the limits set by international regulatory agencies. However, the risk analysis for the population revealed some HQ values above one, particularly for crab consumption.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"5416-5431"},"PeriodicalIF":3.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Iron Alleviates Dextran Sodium Sulfate-Induced Intestinal Injury by Regulating Regeneration of Intestinal Stem Cells in Weaned Mice.","authors":"Xianglin Zeng, Lanmei Yin, Yitong Zhang, Qianqian Wang, Jun Li, Yuebang Yin, Qiye Wang, Jianzhong Li, Huansheng Yang","doi":"10.1007/s12011-025-04546-9","DOIUrl":"10.1007/s12011-025-04546-9","url":null,"abstract":"<p><p>Iron deficiency is the most common comorbidity of inflammatory bowel disease (IBD), but the effect of iron supplementation on the repair processes of intestinal injury in weaned mice is unknown. This study aimed to evaluate the potential mechanism of dietary iron on intestinal injury and intestinal regeneration in the dextran sodium sulfate (DSS)-induced colitis of the weaned mouse model. The mice were fed either a control diet containing (45.00 mg/kg Fe) or iron supplemental (448.30 mg/kg Fe) diet for 14 days, followed by a 7-day oral administration of 2.5% DSS to all mice. The result showed that at day 0 of the recovery period (0 DRP), the impact of iron on the gut index and intestinal morphology was found to be more significant in weaned mice compared to adult mice. At 3 DRP, the iron diet alleviated inflammation-induced weight loss, shortening of colon length, thickening of the muscle layer, and disruption of gut morphology. At 0, 3, and 7 DRP, we found that an iron diet increased intestinal stem cell (ISC) viability and protected epithelial integrity. Furthermore, FeSO₄ significantly enhanced organoid viability and increased mRNA expression of differentiation, ISC, and retinol metabolism-related marker genes in the organoids compared with the control group. Overall, this study demonstrates that the iron diet accelerates intestinal regeneration after intestinal injury in weaned mice by activating the retinol metabolic pathway to regulate the proliferation and differentiation of ISCs.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"5219-5234"},"PeriodicalIF":3.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}