Biological Trace Element Research最新文献

{"title":"Trace Element and Phytochemical Characterization of Pinus pinaster and Pinus halepensis Bark Extracts with Protective Effects Against Phenylhydrazine-induced Hemolytic Anemia in Rats.","authors":"Widad Tbatou, Maouane Aouji, Marouane Takie, Abderrazak Aboulghazi, Akissi Zachée Louis Evariste, Bruno Eto, Badiaa Lyoussi, Zineb Benziane Ouaritini","doi":"10.1007/s12011-026-05194-3","DOIUrl":"https://doi.org/10.1007/s12011-026-05194-3","url":null,"abstract":"<p><p>Anemia, characterized by decreased hemoglobin levels and impaired oxygen-carrying capacity, remains a major global public health concern. Conventional therapies, such as blood transfusions and oral iron supplementation, are often associated with adverse effects and limited absorption, highlighting the need for safer and more effective alternatives. Natural products rich in polyphenols and essential minerals have attracted increasing attention due to their multifunctional therapeutic potential. In this context, the present study is the first to investigate the anti-anemic potential of Pinus pinaster Aiton and Pinus halepensis Mill. bark extracts through an integrated phytochemical, nutritional, in vivo, in vitro, and in silico approach. Phytochemical profiling by HPLC-MS/MS revealed several major phenolic compounds, including quercetin, catechin, protocatechuic acid, and taxifolin-3-O-glucoside. The extracts exhibited high total polyphenol and flavonoid contents and strong antioxidant activity, as demonstrated by DPPH, ABTS, PR, and TAC assays. Mineral analysis revealed significant levels of essential elements, including iron, magnesium, potassium, sodium, and zinc. In phenylhydrazine-induced anemic Wistar rats, 21-day treatment with the extracts significantly restored hematological parameters (RBC, Hb, and HCT), serum iron and ferritin levels, and improved renal and hepatic biomarkers compared with untreated anemic controls. In vitro assays further demonstrated anti-hemolytic activity and enhanced erythrocyte membrane stability. Molecular docking analysis supported these findings by revealing favorable interactions between the major phenolic compounds and key targets involved in erythropoiesis, iron metabolism, and oxidative stress, including the erythropoietin receptor, superoxide dismutase, and ferrochelatase. Collectively, these findings highlight the potent anti-anemic and protective effects of Pinus bark extracts and suggest their potential as promising natural therapeutic agents.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149164270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Effect of Administration of Selenium Nanoparticles Synthesized Using Onion Extract on Biochemical and Inflammatory Parameters in Mice Fed with High‑Fructose Diet: In Vivo and In Silico Analysis.","authors":"Fernando Martínez-Esquivias, Alejandro Perez-Larios, Juan Manuel Guzmán-Flores","doi":"10.1007/s12011-025-04912-7","DOIUrl":"https://doi.org/10.1007/s12011-025-04912-7","url":null,"abstract":"","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc-Mediated Modulation of Host-Pathogen Interactions in Poultry: Implications for Salmonella Control and Trace Element Biology.","authors":"M Naeem","doi":"10.1007/s12011-026-05324-x","DOIUrl":"https://doi.org/10.1007/s12011-026-05324-x","url":null,"abstract":"<p><p>Salmonella remains a major foodborne pathogen associated with poultry production, posing significant public health and economic challenges worldwide. Increasing restrictions on antibiotic use have intensified the search for alternative control strategies in poultry systems. Zinc, an essential trace mineral, has emerged as a promising nutritional intervention due to its multifunctional roles in host defense and microbial regulation. This narrative review synthesizes current evidence on the effectiveness of zinc in reducing Salmonella in poultry, with emphasis on underlying mechanisms, comparative efficacy of different zinc sources, and practical applications in production systems. Available evidence indicates that zinc enhances innate and adaptive immune responses, preserves intestinal barrier integrity, modulates gut microbiota toward beneficial populations, and exerts direct antibacterial effects. These combined actions position zinc as a key regulator of host-pathogen interactions, linking trace element homeostasis with immune function and microbial dynamics. Consequently, zinc supplementation is associated with reduced cecal colonization, decreased fecal shedding, and limited systemic dissemination. Compared with conventional inorganic zinc sources, many organic zinc complexes have demonstrated improved bioavailability and enhanced functional responses under comparable experimental conditions. However, the magnitude of these benefits varies according to ligand chemistry, dietary composition, bird age, physiological status, and study design. Zinc is most effective when integrated with complementary strategies, including probiotics, organic acids, and vaccination programs. Overall, zinc represents a practical component of sustainable, antibiotic-free approaches to improving poultry health and food safety, although further field validation and optimization are required to ensure consistent performance under commercial conditions.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149147071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Carvacrol Modulates Cadmium-Induced Behavioral, Biochemical, Histopathological, and Metabolomic Alterations in rats.","authors":"Nigar Abbak, Emirhan Nemutlu, Tuba Reçber, Asli San Dagli Gul, Fatma Bahar Sunay, Okan Arihan","doi":"10.1007/s12011-026-05355-4","DOIUrl":"10.1007/s12011-026-05355-4","url":null,"abstract":"","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149126657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Protective Effects of Metformin and Memantine Against Arsenic-Induced Neurotoxicity: Insights from In-Silico and In-Vivo Studies.","authors":"Noor Ul Huda Khola, Mahnoor Hayat, Imtisal Muzaffar, Touqeer Ahmed","doi":"10.1007/s12011-026-05353-6","DOIUrl":"https://doi.org/10.1007/s12011-026-05353-6","url":null,"abstract":"","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Dietary Boron Supplementation on Performance, Egg Quality, and Physiological Responses in Late-Laying Hens Subjected to Feed Restriction.","authors":"Soner Uysal, Şeyma Taş, Elif Erbaş, Ayşe Uysal","doi":"10.1007/s12011-026-05223-1","DOIUrl":"10.1007/s12011-026-05223-1","url":null,"abstract":"<p><p>This study aimed to evaluate how different levels of boric acid (BA) supplementation affect performance, serum biochemistry, endoplasmic reticulum (ER) stress, and intestinal structure in late-laying hens subjected to feed restriction. We randomly assigned 180 laying hens, 70 weeks old, into six groups: a non-feed-restricted control group (CON1), non-feed-restricted groups supplemented with 60 mg/kg feed (B60-1) and 90 mg/kg feed (B90-1) of BA, a feed-restricted control group (CON2), and feed-restricted groups supplemented with 60 mg/kg feed (B60-2) and 90 mg/kg feed (B90-2) of BA. The experiment lasted 56 days. Feed restriction significantly reduced egg production and body weight (p < 0.05). However, feed efficiency improved in the feed-restricted control group (CON2) (p < 0.05). In serum biochemical analyses, Alanine transaminase (ALT) levels were lower in the B60-1 group, while triglyceride and total cholesterol levels were lowest in the B90-2 group. Uric acid levels were highest in the CON2 group (p < 0.05), and total protein levels decreased in the B90-2 group (p < 0.05). Nrf-2, GCLC, and GCLM levels were higher in the control groups (CON1 and CON2) compared with BA-supplemented groups (p < 0.01). ATF-4, ATF-6, IRE-1, and CHOP levels significantly increased in the feed-restricted control group (CON2) and decreased with BA supplementation (p ≤ 0.005). While we did not observe significant differences in duodenal structure (p > 0.05), feed restriction caused reductions in villus height and epithelial height in the jejunum and ileum. These reductions were partially improved in the B60-2 and B90-2 groups (p < 0.05). In conclusion, BA supplementation during feed restriction may help maintain intestinal integrity, adjust cellular stress responses, and support performance-related metabolic changes in late-laying hens.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7787-7800"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148390081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Mineral and Trace Element Contents in Commercial Zero-Labeled and Regular Non-Alcoholic Beverages.","authors":"Ga-Eun Choi, Mi-Hyun Kim","doi":"10.1007/s12011-026-05197-0","DOIUrl":"10.1007/s12011-026-05197-0","url":null,"abstract":"<p><p>The consumption of zero-labeled beverages has increased as an alternative to sugar-rich beverages. Although these products are primarily characterized by reduced energy and sugar contents, their mineral and trace element compositions may vary depending on formulation-related factors. The mineral and trace element contents of zero-labeled and regular beverages in Korea were compared. A total of 86 commonly consumed processed beverages were selected as 1:1 matched pairs across major beverage categories. Energy, macronutrient, and sodium data were obtained from nutrition labels, while the contents of macro-minerals (calcium, phosphorus, magnesium, and potassium) and trace minerals (iron, copper, zinc, manganese, molybdenum, chromium, and selenium) were determined by inductively coupled plasma-mass spectrometry. Beverage pH was also measured. Zero-labeled products contained significantly lower energy and sugar levels than regular beverages. Potassium content was significantly higher in zero-labeled carbonated beverages (12.17 vs. 6.73 mg/100 g), and both the potassium and molybdenum contents were higher in zero-labeled energy drinks. Zinc content was significantly lower in zero-labeled mixed beverages. Selenium content was higher in zero-labeled beverages than in regular beverages overall (1.88 vs. 0.87 µg/100 g), particularly in carbonated beverages, sports drinks, and mixed beverages. Other minerals and trace elements did not differ significantly between the groups overall. Beverage pH did not differ between the groups, but was positively correlated with potassium, manganese, and chromium contents. These findings indicate that zero-labeled beverages do not substantially differ from regular beverages in overall mineral and trace element provision but potentially contribute to greater dietary exposure to select minerals in specific beverage categories.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7413-7425"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148359131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyzing the Role of Essential Minerals in Neuropsychiatric Health: A Comparative Assessment of Depressed Versus Healthy Individuals.","authors":"Maqbool Ahmed Soomro, Sanaullah Abbasi, Abdul Majid, Wahid Bux Jatoi","doi":"10.1007/s12011-026-05246-8","DOIUrl":"10.1007/s12011-026-05246-8","url":null,"abstract":"<p><strong>Background: </strong>Emerging evidence suggests a critical link between micronutrient deficiencies and mental health. This study investigated the relationship between serum levels of Magnesium (Mg), Iron (Fe), Zinc (Zn), and Selenium (Se) and the severity of depression among adults in the Sukkur region of Pakistan.</p><p><strong>Methodology: </strong>A comparative cross-sectional study was conducted with 215 participants (105 males, 110 females). Depression severity was assessed using the PHQ-9 instrument, and serum mineral levels were measured using Photometric techniques. Following baseline analysis, a targeted interventional phase was initiated with 40 severely depressed participants, who received a one-month regimen of mineral supplements. Statistical analysis was performed using ANOVA-Tukey and Pearson correlation (p < 0.05).</p><p><strong>Results: </strong>Baseline results revealed a significant inverse correlation between mineral concentrations and depression severity, with the strongest correlations observed for Magnesium (r = -0.771) and Selenium (r = -0.735). Gender-stratified data showed that females generally possessed lower mineral stores, particularly within the severe depression category (Mg: 1.50 ± 0.21 mg/dL). Following the four-week supplementation period, a statistically significant reduction in mean symptom scores was recorded; mean PHQ-9 scores decreased by approximately 50%, falling from \"Severe\" (~ 18.5) to \"Mild\" (~ 8.5) levels. This psychological recovery mirrored the significant restoration of serum mineral levels, with Magnesium rising from 1.26 ± 0.01 to 2.23 ± 0.02 mg/dL and Zinc increasing from 46.45 0.53 to 76.95 ± 0.88 µg/dL.</p><p><strong>Conclusion: </strong>The findings confirm that mineral depletion is strongly associated with the severity of depression. The significant attenuation of depressive symptoms observed during the exploratory targeted supplementation phase suggests the potential value of nutritional therapy as a supportive adjunct or secondary intervention strategy for Major Depressive Disorder (MDD).</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"8089-8106"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148496949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cadmium Exposure and Osteoporosis: A Spatially Contextualized Adverse Outcome Pathway Integrating Epidemiology, Toxicogenomics and Transcriptomics.","authors":"Ye Tong, Baicheng Wan, Gaofeng Zeng, Shaohui Zong","doi":"10.1007/s12011-026-05249-5","DOIUrl":"10.1007/s12011-026-05249-5","url":null,"abstract":"<p><p>Cadmium is an environmentally relevant metal toxicant associated with impaired bone health, but the mechanistic architecture linking cadmium exposure to osteoporosis remains insufficiently organized. We developed a cadmium-centered, adverse outcome pathway (AOP)-guided framework by integrating population epidemiology, toxicogenomics, bulk and single-cell transcriptomics, inferred myeloid pseudotime, and spatial transcriptomics. Survey-weighted analyses of NHANES 2013-2014 and 2017-2018 showed that whole-blood cadmium was positively associated with osteoporosis (odds ratio, 1.43; 95% confidence interval, 1.05-1.95), with an increasing exposure-response pattern. In quantile g-computation using total femur bone mineral density as the outcome, cadmium contributed the largest negative-direction weight. Cadmium-related toxicogenomic evidence from the Comparative Toxicogenomics Database was organized into six biologically interpretable key-event modules. Bulk transcriptomic analyses revealed compartment-skewed module representation in femoral tissue and peripheral monocytes, whereas single-cell analysis localized module scores mainly to stromal and myeloid populations. Myeloid pseudotime analysis identified three associated gene programs reflecting innate defense, interferon activation, and remodeling/lipid handling. Spatial transcriptomics further revealed niche-associated module distributions, trabecula-related gradients, and nonrandom spatial clustering, particularly for bone-remodeling, inflammation/cell-fate, and oxidative stress/mitochondrial modules. These findings provide a spatially contextualized, hypothesis-generating AOP framework that organizes cadmium-osteoporosis association evidence with osteoporosis-relevant molecular and spatial patterns. The proposed relationships require validation in longitudinal exposure studies and experimental models.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"8143-8163"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148519909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal Dynamics of Trace Mineral Prioritization and Retention in a Bamboo-feeding Specialist: The Giant Panda (Ailuropoda melanoleuca).","authors":"Yan Shu, Fengmei Wang, Jinchao Tan, Dapeng Zhu, Leigang Zhao, Tingting Fang, Shibin Yuan, Le Wang","doi":"10.1007/s12011-026-05251-x","DOIUrl":"10.1007/s12011-026-05251-x","url":null,"abstract":"<p><p>The giant panda (Ailuropoda melanoleuca) is an obligate bamboo specialist and exhibits pronounced seasonal shifts in diet composition. To investigate how wild pandas respond to seasonal variations in dietary trace minerals, we quantified four essential elements-iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu)-in the dominant bamboo species (Bashania fargesii and Fargesia qinlingensis) and in panda feces over a full annual cycle in Foping National Nature Reserve, China. Using nutritional geometry (Geometric Framework, GF) and the Right-Angled Mixture Triangle (RMT) model, we assessed seasonal intake priorities, key elemental ratios (Cu/Mn, Cu/Zn), and relative mineral utilization. Trace mineral prioritization was strongly time-dependent: during the breeding, leaf-feeding, and overwintering periods, the prioritization pattern was Cu > Zn > Mn, whereas during the shoot-feeding stages of both bamboo species, it shifted to Cu > Mn > Zn. Copper consistently emerged as the highest ingestion priority mineral across all seasons, while iron consistently showed the lowest ingestion priority. The Cu/Mn and Cu/Zn ratios varied dynamically with dietary shifts: Cu/Mn was significantly higher in shoots than in leaves, and Cu/Zn peaked in April (Bashania shoots) and August (Fargesia leaves). Compared with wild individuals, captive pandas in Sichuan exhibited substantially higher Fe intake relative to Cu (77-623 mg Fe per 1 mg Cu vs. 13-29 mg Fe per 1 mg Cu in wild pandas), indicating a potential risk of iron overload. We conclude that wild giant pandas maintain trace mineral balance by synchronizing seasonal migration and dietary switching with fluctuations in bamboo mineral profiles. Our results emphasize the conservation importance of maintaining bamboo diversity in situ and highlight the need to refine captive feeding protocols to mimic wild seasonal nutritional patterns, particularly with respect to Fe regulation.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"8186-8195"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148878875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}