Biological Trace Element Research最新文献

{"title":"Development and Validation of an ICP-MS/MS Method for Multi-elemental Analysis of Human Hair with Application to Heart Failure.","authors":"Pablo Ramírez-Castillo, Alba García-Suarez, Pedro Morillas-Blasco, Nuria Vicente-Ibarra, Antonio García-Honrubia, Marina Martínez-Moreno, Raquel Sánchez-Romero, Ana Beltrán-Sanahuja, José-Luis Todolí-Torró","doi":"10.1007/s12011-026-05217-z","DOIUrl":"10.1007/s12011-026-05217-z","url":null,"abstract":"<p><p>Human hair has emerged as a promising non-invasive matrix for assessing long-term exposure to essential and toxic trace elements relevant to cardiovascular disease. However, its clinical applicability depends on the availability of robust and fully validated analytical methodologies capable of overcoming matrix-related challenges. This study aimed to develop and validate a reliable and high-throughput method for multi-elemental (As, Cd, Cu, Pb, Se and Zn) analysis of human hair using microwave-assisted acid digestion followed by ICP-MS/MS, and to explore its potential application in a pilot cohort of heart failure (HF) patients. Method validation was performed using the certified reference material ERM-DB001. Analytical performance was evaluated in both no-gas and He-KED modes, assessing precision, trueness, procedural detection limits, and measurement uncertainty according to EURACHEM/CITAC guidelines. The influence of sample mass (20-200 mg) was also investigated. The validated method was subsequently applied to hair samples from 25 HF patients and 25 control subjects. The method demonstrated satisfactory intra- and inter-day precision, particularly for Cd, Cu, Se, and Zn. He-KED mode effectively reduced polyatomic interferences, improving selectivity and lowering detection limits for elements such as As and Se. Optimal analytical performance was achieved for sample masses between 60 and 100 mg, while lower masses resulted in increased uncertainty. In the pilot application, exploratory differences were observed for Pb, Mg, Ca and Zn concentrations, together with changes in selected elemental correlations (Mg/Na, Pb/Mn, Sr/Ni, Fe/Al, Fe/Ti and Mn/Ti) between heart failure patients and controls. The validated ICP-MS/MS method provides a sensitive and reliable approach for multi-elemental analysis of human hair. Its robustness and applicability to real samples support its potential use in clinical and epidemiological studies, although further research with larger cohorts is required to confirm the observed trends.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7711-7720"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13615901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148390096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potentially Toxic Metal Levels in Cheeses Produced in Türkiye: Regional Variation, Milk Source, Ripening Stage, Production Type, and Associated Dietary Exposure and Health Risk.","authors":"Hüseyin Ender Gürmeriç, Mustafa Şengül, Burhan Basaran","doi":"10.1007/s12011-026-05198-z","DOIUrl":"10.1007/s12011-026-05198-z","url":null,"abstract":"<p><p>This study investigated potentially toxic metal (PTM) levels in cheeses produced in different regions of Türkiye and evaluated the associated dietary exposure and non-carcinogenic health risk. A total of 84 cheese samples were collected from seven geographical regions and classified according to milk source, ripening stage, and production type. After microwave-assisted acid digestion, PTM levels were quantified by inductively coupled plasma mass spectrometry (ICP-MS), and exposure and non-carcinogenic risk were evaluated using Estimated Daily Intake (EDI), Target Hazard Quotient (THQ), and Hazard Index (HI). Aluminium (Al), iron (Fe), and zinc (Zn) ranged from <0.00215 to 7.29,<0.00769 to 38.8, and 0.68 to 56.6 mg/kg wet weight, respectively, whereas chromium (Cr), nickel (Ni), copper (Cu), selenium (Se), cadmium (Cd), and lead (Pb) ranged from <0.58 to 244, <1.26 to 369, <1.88 to 4067, <0.91 to 1378, <0.06 to 12.3, and <2.19 to 1754 µg/kg wet weight, respectively. Values marked with \"<\" represent concentrations below the limit of quantification (LOQ). Arsenic (As) was not detected in any sample. Significant differences were observed for selected PTMs according to ripening stage, milk source, production type, and region (p<0.05). The toxicological contribution (TC) values for nutritional PTM exposure were low. THQ and HI values remained below 1, suggesting a low overall non-carcinogenic risk; however, Pb exceedances in some samples indicate the need for continued PTM monitoring.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7426-7440"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13615985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148343873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental Toxicants and Polycystic Ovary Syndrome: Implications for a Metabolic-Ovarian Framework (PMOS) - A Systematic Review.","authors":"James O Ibibama, Polycarp Dauda Madaki, Kenechi Adachi Aliche, Chiara Frazzoli, Orish E Orisakwe","doi":"10.1007/s12011-026-05218-y","DOIUrl":"10.1007/s12011-026-05218-y","url":null,"abstract":"<p><strong>Background: </strong>Polycystic ovary syndrome (PCOS) is increasingly recognized as a systemic condition characterized by intricate endocrine and metabolic dysregulation. Emerging evidence suggests that environmental contaminants may drive its pathogenesis, supporting an expanded conceptualization of the disorder as polycystic metabolic-ovarian syndrome (PMOS). This systematic review aimed to evaluate the association between exposure to potentially toxic elements (PTEs) and endocrine disruptors and the reproductive, hormonal, and metabolic clinical features of PCOS.</p><p><strong>Methods: </strong>Following PRISMA guidelines, a systematic search was conducted across PubMed, Embase, Scopus, and Web of Science for human observational studies published up to 31 December 2025. Methodological quality was appraised using the Newcastle-Ottawa Scale (NOS). Due to heterogeneity in biological matrices and toxicant classes, a narrative synthesis was performed.</p><p><strong>Results: </strong>Fourteen high-quality studies (NOS scores 8-9) from seven countries met the inclusion criteria. The evidence consistently linked exposure to endocrine disrupting chemicals specifically bisphenol A (BPA) and phthalates-with hyperandrogenism, altered luteinizing hormone/follicle-stimulating hormone ratios, and impaired ovarian reserve. Furthermore, heavy metals (cadmium, lead, mercury, arsenic) and emerging rare earth elements were significantly elevated in women with PCOS across multiple matrices (serum, urine, and follicular fluid). These exposures were strongly associated with metabolic disturbances, including insulin resistance, obesity indices, and lipid abnormalities, mediated predominantly by oxidative stress pathways and impaired antioxidant defences.</p><p><strong>Conclusion: </strong>Current observational evidence suggests associations between exposure to selected environmental toxicants and endocrine‑metabolic features of PCOS. However, heterogeneity in exposure assessment, biological matrices, PCOS diagnostic criteria, and confounder adjustment limits causal interpretation. Future prospective studies with repeated biomonitoring are needed.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7721-7734"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148418256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Serum Lithium Levels with Insulin Resistance in Type-2 Diabetes Patients.","authors":"Pramod Kumar, Rasna Gupta, Manoj Yadav, Rahul Patel, Mahendra Kumar Meena, Ankit Gupta","doi":"10.1007/s12011-026-05203-5","DOIUrl":"10.1007/s12011-026-05203-5","url":null,"abstract":"<p><p>Insulin resistance arises from impaired insulin signaling, leading to reduced glucose uptake and the progression of type 2 diabetes mellitus. Experimental evidence indicates that lithium modulates insulin signaling pathways; however, its role in the development of insulin resistance remains poorly defined. Anthropometric, biochemical, and lifestyle data were collected. IR was assessed via HOMA-IR, and insulin processing/clearance was evaluated by insulin to c-peptide ratio (ICPR). Univariate and multivariate logistic regression identified independent predictors of IR. ROC analysis assessed the diagnostic performance of Li. Linear regression examined associations between biochemical parameters and IR. A cross-sectional study was conducted among 211 adults with T2D attending AIIMS Raebareli; of these, 172 participants with complete data constituted the final analytical cohort, including 122 (70.9%) with insulin resistance (IR). The IR group exhibited significantly higher fasting and postprandial glucose, insulin, C-peptide, ICPR, total cholesterol, and TyG index, and lower serum lithium levels than the non-IR group. Multivariate logistic regression analyses identified ICPR, TyG index, and serum lithium (OR = 3.69, 95% CI: 1.190-11.437, P = 0.024) as independent predictors of IR. ROC analysis showed that a lithium cutoff of 0.1 mmol/L predicted IR, with 81.9% sensitivity and 56.5% specificity (AUC = 0.863). Lower lithium levels were associated with higher insulin, C-peptide, and ICPR, and linear regression revealed a significant inverse association between lithium and HOMA-IR (β = -0.233, p = 0.007). Lower serum lithium levels independently predict insulin resistance in T2DM, alongside ICPR and TyG index. This association with hyperinsulinemia and impaired insulin processing/clearance suggests a potential mechanistic role for lithium in metabolic dysregulation.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7501-7510"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148343879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomonitoring of Toxic Metals and Metalloids in Hair and Nails of Fishermen from Lake Hawassa, Ethiopia.","authors":"Danait Hadera Gebremedhin, Solomon Sorsa Sota, Mekuria Teshome Mergia","doi":"10.1007/s12011-026-05224-0","DOIUrl":"10.1007/s12011-026-05224-0","url":null,"abstract":"<p><p>Contamination of aquatic ecosystems by toxic metals and metalloids poses potential health risks to fishing communities with high fish consumption. This study assessed arsenic (As), mercury (Hg), chromium (Cr), and copper (Cu) concentrations in paired hair and nail samples from 50 fishermen on Lake Hawassa, Ethiopia, using MP-AES. Descriptive statistics, Wilcoxon signed-rank tests, correlation analyses, and multiple linear regression were applied to evaluate elemental concentrations, relationships between biomarkers, and the influence of age and fishing experience. Nails generally showed higher elemental levels than hair. Median concentrations in hair and nails were 0.15 and 0.42 µg/g for As, 0.18 and 0.35 µg/g for Hg, 0.43 and 0.70 µg/g for Cr, and 8.73 and 7.73 µg/g for Cu, respectively. Significant differences between biomarkers were found for As (p = 0.029) and Hg (p = 0.038), but not for Cr and Cu. Elevated levels above reference values were more frequent in nails, especially for As (36%), Hg (30%), and Cr (30%). Hair and nail concentrations correlated positively for As (R²=0.15, p = 0.005) and Hg (R²=0.22, p < 0.001). Age and fishing experience were not major determinants, though fishing experience negatively correlated with hair Cr (β=-0.547, p = 0.004), and age with nail Cu (β=-0.511, p = 0.034). The findings confirm measurable exposure to toxic elements and suggest nails may be a more sensitive long-term biomarker than hair. This establishes baseline data and underscores the need for ongoing environmental and health surveillance in the region.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7801-7816"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148403545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Health and Regulatory Assessment of Hydroquinone, Mercury and Steroids in Skin-Lightening Creams Marketed in Pakistan.","authors":"Mahnoor Shujait, M Nawaz Ch, Naqi Hussain","doi":"10.1007/s12011-026-05237-9","DOIUrl":"10.1007/s12011-026-05237-9","url":null,"abstract":"<p><p>People from all over the world use cosmetics to enhance their physical appearance, seeking social acceptance. Most locally produced skin-lightening creams used in developing countries are a mixture of fat and water applied to the skin surface. These Skin Lightening creams may pose a potential health risk due to their inclusion of various highly toxic active ingredients like hydroquinone, mercury, and steroids. This study quantifies the levels of these toxic substances in ten commonly used SLC brands in Pakistan and their associated health risks in samples marketed in Lahore. To determine the concentration levels of various ingredients, including hydroquinone, steroids (Betamethasone, Dexamethasone, Hydrocortisone, and Prednisolone), and mercury, high-performance liquid chromatography, ultra violet- high-performance liquid chromatography, and a direct mercury analyzer were used, respectively. Health risks were assessed by calculating Chronic Daily Intake (CDI) and Hazard Quotient (HQ). Results showed that some samples exceeded international limits for hydroquinone (up to 5.56%) and mercury (up to 4.9 ppm), surpassing U.S Food and Drug Administration (USFDA), Pakistan Standards and Quality Control Authority (PSQCA), and World Health Organization (WHO) standards. The analysis also revealed undeclared corticosteroids in several samples: betamethasone (0.6-1.8% in 30% of samples), dexamethasone (1.4-6.4% in 20% of samples), hydrocortisone (0.4-8.5% in 50% of samples), and prednisolone (0.3-1.6% in 30% of samples). These findings suggest the need for improved regulatory enforcement in developing countries along with enhanced monitoring of Pakistan's cosmetic industry to effectively protect public health from the cumulative toxicity of hydroquinone, mercury, and corticosteroids.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7976-7988"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148560850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Terminalia arjuna Bark Nanosuspension Attenuates Fluoride-Induced Cardiotoxicity in Rats by Modulating Oxidative Stress, Inflammation and ACE Expression.","authors":"Senthilkumar Keerthana, Palanisamy Sankar, Arivalagan Arivuchelvan, Kuppusamy Gopal","doi":"10.1007/s12011-026-05238-8","DOIUrl":"10.1007/s12011-026-05238-8","url":null,"abstract":"<p><p>Fluoride exposure through drinking water is an independent risk factor for cardiovascular disease. Terminalia arjuna is well known for its cardioprotective properties, including positive inotropic effects, hypolipidemic activity and coronary vasodilation. This study aimed to evaluate the effect of T. arjuna bark nanosuspension on fluoride-induced cardiotoxicity in rats. The nanosuspension was prepared using polysorbate-80 as an aqueous stabilizer and exhibited uniform particles with a mean size of 118.0 nm. Forty rats were divided into five groups: Group I served as the control, while the other four groups were exposed to sodium fluoride (100 ppm) daily through drinking water for 28 days. Group III received 200 mg/kg T. arjuna bark extract, while Groups IV and V were administered T. arjuna bark nanosuspension at doses of 100 mg/kg and 50 mg/kg, respectively. Fluoride administration caused significant increases in blood pressure and serum biochemical markers, along with decreased cardiac antioxidant defenses, in rats compared with the control group. Fluoride exposure also increased angiotensin-converting enzyme (ACE), nuclear factor kappa B (NF-κB), and tumor necrosis factor alpha (TNF-α) levels, as well as altered the histology and immunohistochemical expression (Bcl-2 and caspase-9) of cardiac tissue. Treatment with T. arjuna bark nanosuspension significantly attenuated these fluoride-mediated cardiotoxic effects and preserved the normal histological architecture of the cardiac tissue. However, the magnitude of these effects indicates that T. arjuna bark nanosuspension at a dose of 100 mg/kg has greater ameliorative potential.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7989-8000"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Integrated in Silico, in Vitro, and in Vivo Assessment of the Antioxidant and Neuroprotective Profile of Selenocyanate Compounds.","authors":"Maria Eduarda Ribeiro da Rocha, Bruna Rafaela Pizzi, Pâmella da Costa, Tácia Katiane Hall, Mariana Parron Paim, Pâmella Cordeiro, Victor Menezes, Vanessa Nascimento, César Augusto Brüning, Paloma Taborda Birmann, Cristiani Folharini Bortolatto","doi":"10.1007/s12011-026-05247-7","DOIUrl":"10.1007/s12011-026-05247-7","url":null,"abstract":"<p><p>Cyanate-based compounds have received increasing attention due to their neuroprotective and antioxidant properties, which can be further enhanced by the incorporation of selenium, a key element in redox regulation. In this study, five selenocyanate derivatives (1-5), differing by alkyl or aryl groups, were synthesized and systematically evaluated using an integrated in silico, in vitro, and in vivo approach to explore their pharmacological and toxicological potential. Computational analyses included predictions of pharmacokinetic and toxicological parameters (ADME/T), as well as molecular docking studies targeting neurotransmitter-related enzymes, namely acetylcholinesterase (AChE) and monoamine oxidases (MAO-A and B). Antioxidant activity was evaluated using multiple complementary in vitro assays, including DPPH, ABTS, FRAP, GST-like, and SOD-like, as well as cerebral reactive species (RS). Cerebral AChE, MAO-A, and MAO-B activities were also measured in vitro. In vivo experiments involved evaluating acute oral toxicity in female Swiss mice. In silico results indicated favorable ADME/T profiles and affinity of selenocyanates for the target neurochemical enzymes. In vitro assays revealed antioxidant capacity for selenocyanates, particularly for compounds 1, 4, and 5. All compounds inhibited cerebral AChE and MAO isoforms in vitro, with particular emphasis on compounds 2 and 4 as AChE inhibitors. Therefore, compounds 2 and 4 were tested in vivo and showed no acute toxicity at an oral dose of 50 mg/kg; however, at 300 mg/kg, compound 2 showed signs of toxicity, whereas compound 4 remained non-lethal. Taken together, these results highlight organoselenocyanates, especially compound 4, as promising candidates for the development of antioxidant and neuroprotective agents.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"8107-8129"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148629575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of Virgin Olive Oil Against Titanium Dioxide Nanoparticle-Induced Hepato-Renal Toxicity: Evidence from Oxidative Stress Biomarkers and Restoration of Antioxidant Enzyme Activities.","authors":"Khaoula Boughediri, Ramzi Triki, Soumaia Boughediri, Said Derbal, Meryem Ouazouaz, Mahfuz Elmastaş, Zafer Omer Ozdemir, Cherifa Henchiri, Houssem Boulebd","doi":"10.1007/s12011-026-05226-y","DOIUrl":"10.1007/s12011-026-05226-y","url":null,"abstract":"<p><p>Titanium dioxide nanoparticles (TiO₂-NPs) are extensively used in industrial and biomedical applications and have been reported to induce hepato-renal toxicity through oxidative stress and inflammation. This study evaluated the protective effects of virgin olive oil (VOO) against TiO₂-NP-induced damage, while characterizing its chemical composition and exploring the molecular interactions of its bioactive compounds. VOO composition was analyzed by UV-visible spectrophotometry, HPLC-PDA, and GC-FID, and its antioxidant activity was assessed using the DPPH assay. Twenty-four male Wistar rats were divided into four groups (n = 6): a control group and three TiO₂-NP-exposed groups, two of which received VOO at 2 and 4 g/kg body weight, respectively, starting 24 h post-exposure. After six weeks, hematological, biochemical, oxidative stress, and histopathological parameters were evaluated. Molecular docking was performed to explore interactions between identified phenolics and key antioxidant and inflammatory enzymes (GSTP1-1, COX-2, CAT, and 5-LOX). VOO exhibited notable antioxidant activity (DPPH inhibition: 54.80%) and a high content of phenolic compounds, including quercetin, rutin, and 4-hydroxybenzoic acid. TiO₂-NP exposure induced significant oxidative stress, evidenced by increased malondialdehyde levels, decreased glutathione content, reduced antioxidant enzyme activities (GPx, GST, and CAT), and altered hematological parameters. VOO supplementation significantly attenuated these alterations in a dose-dependent manner, partially restored antioxidant defenses, and improved biochemical and hematological profiles, while helping to preserve tissue architecture. Docking analysis revealed notable binding affinities of phenolic compounds toward target enzymes, suggesting interactions consistent with the in vivo findings. In conclusion, VOO may mitigate TiO₂-NP-induced hepato-renal toxicity in rats, possibly through antioxidant and anti-inflammatory mechanisms. These effects are likely associated with its phenolic compounds, as suggested by both in-vivo and in silico findings.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7833-7850"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148418247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stratified Reference Intervals for Serum Selenium by Selenium Environment, Health, and Physiological Status in Populations from Shaanxi Province, China.","authors":"Zhe Yang, Xianshun Gao, Meng Qu, Xiaoping Du, Hui Xie, Xiaohong Li, Rongli Lian, Chen Chen, Qun Chen","doi":"10.1007/s12011-026-05235-x","DOIUrl":"10.1007/s12011-026-05235-x","url":null,"abstract":"<p><p>Serum selenium (Se) is a core indicator for evaluating human selenium nutritional status. In Shaanxi Province, considerable geographical variation in selenium environment distribution limits the applicability of generalized reference intervals, which do not account for regional or physiological differences. This study aimed to establish stratified serum/hair Se reference intervals for populations with different physiological statuses across high-Se, adequate-Se, and low-Se regions in Shaanxi Province, China, and to identify key influencing factors, thereby providing region-specific evidence for selenium monitoring and intervention. This study integrated multiple datasets from Shaanxi Province, China, including pregnant women, healthy adults, hospitalized patients, and children aged 7-12 years, and comprised two components: (1) a multicenter observational analysis to establish stratified serum and hair selenium reference intervals and evaluate environmental factors, and (2) a selenium supplementation intervention in children from a low-selenium region to assess changes in hair selenium after supplementation. Multiple sets of stratified reference intervals were established: 32.7-96.5 µg/L for healthy adults (adequate-Se), 30.1-86.7 µg/L for healthy women, 32.8-89.7 µg/L for pregnant women (high-Se), 33.1-68.2 µg/L for pregnant women (medium-to-high Se composite), 35.2-88.2 µg/L for elderly hospitalized patients (adequate-Se), and 0.2-0.5 µg/g for hair Se in supplemented children (low-Se). Region, age, and liver/kidney function were identified as core influencing factors. Hair Se levels in children from low-Se regions were significantly increased after Se supplementation. This study established stratified Se reference intervals for populations with different Se environments and health/physiological statuses in Shaanxi Province, China, providing region-specific evidence for selenium assessment and for cautiously tailored nutritional intervention in comparable settings.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7940-7955"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148519865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}