Biological Trace Element Research最新文献

{"title":"Coenzyme Q10 Attenuates Kidney Injury Induced by Titanium Dioxide Nanoparticles and Cadmium Co-exposure in Rats.","authors":"Yasmina M Abd-Elhakim, Mohamed M M Hashem, Khaled Abo-El-Sooud, Abeer E El-Metawally, Bayan A Hassan","doi":"10.1007/s12011-024-04469-x","DOIUrl":"10.1007/s12011-024-04469-x","url":null,"abstract":"<p><p>This study examined the possible defensive role of coenzyme Q10 (CQ10) against the impact of cadmium (Cd) and titanium dioxide nanoparticle (TNP) exposure on rat kidneys. Distilled water (1 mL/rat), corn oil (1 mL/rat), 10 mg CQ10/kg b.wt, 50 mg TNP/kg b.wt, 5 mg Cd/kg b.wt, TNP + Cd, or TNP + Cd + CQ10 was administered orally to seven groups of 70 male Sprague Dawley rats for 60 days. The findings demonstrated that TNP and/or Cd exposure considerably raised serum levels of several renal damage products, disturbed electrolyte balance including sodium, potassium, and calcium, decreased antioxidant enzyme concentration in the kidneys, and elevated malondialdehyde. In addition, rats exposed to TNP and/or Cd had significantly higher levels of renal titanium and Cd. In addition, rats exposed to TNP and/or Cd showed significant histopathological lesions and collagen deposition as revealed by H and E and Masson trichrome staining, respectively. The kidneys were severely damaged by the combined effects of TNP and Cd, although CQ10 greatly mitigated these effects. According to the study, exposure to TNP and Cd can damage the kidneys' function and structure, especially when combined. However, CQ10 can protect against TNP and Cd's nephrotoxic effects.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4183-4197"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12174286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium Biofortification of Vegetables Grown in Calcareous Soil: A Pot Experiment Using ⁷⁷Se as a Tracer.","authors":"Abdolbaset B K Baset, Karzan A M Hawrami, Elizabeth H Bailey, Scott D Young","doi":"10.1007/s12011-024-04483-z","DOIUrl":"10.1007/s12011-024-04483-z","url":null,"abstract":"<p><p>Dietary selenium (Se) is vital for human health and can be provided through consumption of Se-rich vegetables. Soil Se is often poorly available and so biofortification using Se-enriched fertilizers is used to enhance dietary intake. This study aimed to (a) evaluate the feasibility of biofortifying vegetables, commonly grown in the calcareous soils of Kurdistan, with a single application of Se (10 g ha^-1) as selenate and, (b) trace the fate of applied Se using an enriched stable isotope, ⁷⁷Se. A randomized block pot experiment was conducted with five vegetable species: celery, chard, lettuce, radish, and spring onion. Soils were spiked with 2 µg ⁷⁷Se per pot, simulating 10 g ha^-1. Plants were harvested after 8 weeks, and both plant tissues and soils underwent sequential extraction and isotopic analysis to determine Se fractionation and source apportionment. Across all species, plant uptake of native soil Se (Se_s) exceeded that of fertilizer-derived Se (Se_f). Shoot concentrations of Se_s ranged from 58.2 to 115 µg kg^-1, while ⁷⁷Se_f concentrations varied between 10.5 and 46.9 µg kg^-1. Post-harvest soil analyses indicated immobilization of applied ⁷⁷Se: 55% transitioned to organically bound forms, 40% became recalcitrant, and only 5% remained in plant-available fractions. The study underscores the challenges of Se biofortification in calcareous soils, where interaction with CaCO₃ may reduce Se availability. Variations in Se uptake among vegetable species highlight the importance of application timing. To enhance biofortification efficacy for fast-growing leafy vegetables, mid-season or foliar Se applications are recommended to counteract rapid soil immobilization.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4397-4407"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of Blood Titanium Levels on DNA Damage in Brazilian Workers Occupationally Exposed to Different Chemical Agents.","authors":"Angela M Moro, Natália Brucker, Gabriela Goethel, Ingrid Flesch, Sabrina Nascimento, Mariele Charão, Bruna Gauer, Elisa Sauer, Larissa V Cestonaro, Gabriel Pedroso Viçozzi, Adriana Gioda, Tatiana D Saint'Pierre, Marcelo D Arbo, Ingrid Garcia, Shanda A Cattani, Rodrigo R Petrecelli, Mirkos Ortiz Martins, Solange Cristina Garcia","doi":"10.1007/s12011-024-04472-2","DOIUrl":"10.1007/s12011-024-04472-2","url":null,"abstract":"<p><p>Occupational exposure to pollutants may cause health-damaging effects in humans. Genotoxicity assays can be used to detect the toxic effects of pollutants. In the present study, we evaluated genetic damage in three populations occupationally exposed to benzene, pyrenes, and agrochemicals and assessed the possible influence of titanium (Ti) co-exposure. A total of 275 subjects were enrolled in this study. The occupationally exposed population was composed of 201 male individuals, divided into three different groups: gas station attendants (GSA group) (n = 76), taxi drivers (TD group) (n = 97), farmers (farmers group) (n = 28), and control (n = 74). Biomarkers of exposure and effect were investigated such as AChe, BuChE, t,t-muconic acid (t,t-MA), and 1-hydroxypyrene (1-OHP). Ti levels in blood were higher in all the workers compared with the control group. DNA damage evaluated by comet assay was higher in the taxi drivers and farmers than in the controls, and the frequency of micronucleate buccal cells was higher in the gas station attendants and taxi drivers than in the controls. Correlations were found among occupational exposure time and biomarkers of exposure, genotoxicity biomarkers, and blood Ti levels. Our results demonstrated Ti co-exposure in the gas station attendants, taxi drivers, and farmers, and blood Ti levels were linked with the respective biomarkers of exposure. Additionally, tools through machine learning corroborated these findings, and Ti was the factor that contributed to DNA damage. Thus, the present study indicates the role of Ti in occupational settings and interactions with already known major xenobiotics present in the occupational environment contributing to genotoxicity.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"4013-4026"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and Validation of Sandell-Kolthoff Method for Saliva Iodine Determination.","authors":"Yue Su, Ming Li, Honglei Xie, Xin Hou, Meng Zhao, Jia Li, Haowen Pan, Zexu Zhang, Zongyu Yue, Wenjing Che, Lanchun Liu, Jiasen Cao, Peng Liu","doi":"10.1007/s12011-024-04468-y","DOIUrl":"10.1007/s12011-024-04468-y","url":null,"abstract":"<p><p>Saliva iodine is a useful biochemical marker for evaluating iodine status and controlling iodine-related diseases. This study aims to establish and validate a simple, reliable, and low-cost Sandell-Kolthoff (S-K) method for the determination of iodine in saliva. The method was established by considering the digestion solution type, concentration, dosage, digestion time and temperature, the volume of saliva samples, glass tube size, dosage of arsenious acid solution and ammonium ceric sulfate solution, and warm bath time. Then, the effectiveness of the method was examined by the linearity of the standard curve, detection limit, precision, and accuracy. The 1.5 mL 1 Mol/L ammonium persulfate solution at 100 °C for 90 min, the 200 μL saliva sample, glass tubes of 15 mm × 120 mm, 2.5 mL arsenious acid solution, 0.3 mL ammonium ceric sulfate solution, and 15 min for warm bath was selected to determine saliva iodine. In terms of validation of the S-K method, the linearity method has the linear range of the standard curve from 0 to 300 μg/L and the coefficient of determination ≥ 0.999; the detection limit, when the volume of saliva samples was 0.2 mL, was 4.6 μg/L; for precision, the relative standard deviation was from 0.3 to 2.8%; and for accuracy, the total recovery rate ranged from 92.8 to 107.0%. This S-K method is easy to operate, has good precision and accuracy, and can be used as a method to determine saliva iodine. It has great potential practical value in disease prevention, clinical auxiliary diagnosis, and experimental research.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"3998-4012"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-ZnO Improves IUGR-Induced Abnormal Fat Deposition in Finishing Pigs by Remodeling the Intestinal Microbiota and Regulating Serum Metabolism.","authors":"Minhang Tu, Shun Chen, Longfei Ma, Jiaqi Zhang, Gentan Cai, Tian Wang, Chao Wang","doi":"10.1007/s12011-025-04754-3","DOIUrl":"https://doi.org/10.1007/s12011-025-04754-3","url":null,"abstract":"<p><p>Intrauterine growth retardation (IUGR) in pigs is closely associated with excessive obesity and lipid metabolic abnormalities during postnatal catch-up growth. Nano zinc oxide (Nano-ZnO) is a novel nanomaterial that has antioxidant properties and can improve abnormal lipid metabolism. This study aimed to investigate the weight-reducing effects and mechanisms of Nano-ZnO in IUGR pigs. Six normal birth weight (NBW) and twelve IUGR male piglets were divided into three groups post-weaning (day 22): NBW and IUGR groups received a basal diet, while the IUGR + Zn group was fed a Nano-ZnO-supplemented diet (600 mg/kg). Nano-ZnO significantly reduced backfat thickness and improved fatty acid composition in IUGR pigs. Mechanistically, Nano-ZnO activated the NRF2 signaling pathway, enhancing antioxidant capacity in adipose tissue by elevating T-SOD and T-AOC activities, upregulating SOD/GCLC/GCLM expression, and suppressing KEAP1 and MDA levels. Concurrently, it promoted lipolysis via the adiponectin/AMPK/SREBP1C/PPARγ axis, increasing ACC/CPT-1/HSL activity and mRNA expression while inhibiting FAS. Furthermore, Nano-ZnO modulated gut microbiota composition (e.g., increasing cecal UCG-005, Rikenellaceae RC9, and fecal Phascolarctobacterium) and serum metabolites (e.g., reducing phenylpyruvic acid and 9(10)-EpOME, elevating docosahexaenoic acid and glycochenodeoxycholic acid). These findings demonstrate that Nano-ZnO ameliorates abnormal fat deposition in IUGR pigs through coordinated antioxidant, lipolytic, gut microbial, and metabolic reprogramming.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144727295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Trace Elements and their Health Impacts from Vegetable Consumption in the Vicinity of the Lolodorf Uranium Deposit, Southern Cameroon.","authors":"Bonaventure Mvogo Aloa, Jean Félix Beyala Ateba, Cebastien Joel Guembou Shouop, Jean Marie Ema'a Ema'a, Jean Faustin Sabouang, Dieu Souffit Gondji, Germain Hubert Ben-Bolie","doi":"10.1007/s12011-025-04760-5","DOIUrl":"https://doi.org/10.1007/s12011-025-04760-5","url":null,"abstract":"<p><p>Plants possess the capability to absorb, translocate, and accumulate significant levels of potentially harmful substances, including trace metals, in their edible tissues, posing health risks to consumers. To assess the concentrations of inorganic contaminants, namely trace metals and their associated health impact on humans in commonly consumed vegetables cultivated in the localities of Mvengue and Awanda, near the uranium-bearing area of Lolodorf (Cameroon), nine vegetable varieties were sampled for Energy Dispersive X-ray Spectrometry analysis. The selected vegetables included cassava leaves (LMA), cocoyam leaves (LXA), bitter leaves (LVE), nalta-jute leaves (LCO), amaranth leaves (LAM), cassava tubers (TMA), cocoyam tubers (TXA), lady's fingers (FAB), and banana plantains (FMU). Trace elements in leafy vegetables were found in varying concentrations, predominantly Fe, Mn, and Zn, while in non-leafy vegetables (tubers and plantains), Fe, Zn, and Ni were most common. The mean concentrations of trace elements Al, Cr, Mn, Th, U, Fe, Ni, Cu, and Zn were determined to be (671.1 ± 11), (3.1 ± 0.5), (117.1 ± 0.7), (0.2 ± 0.1), (2.9 ± 0.7), (292.9 ± 1.0), (9.2 ± 0.8), (11.9 ± 0.8), and (43.1 ± 0.8) mg/kg, respectively. When comparing the obtained results with the WHO/FAO permissible level, it appears that Al, Cr, and Fe represent the main cause of vegetable contamination in the investigated areas. Concentrations ranged significantly, with some samples exceeding WHO/FAO permissible levels: 44% were contaminated with Al (LVE, LCO, LAM, and FAB) and Cr (LXA, LVE, LAM, and FAB), 33% with Fe (LVE, LCO, and FAB), and 11% with Mn (LAM). No contamination was observed for Ni, Cu, and Zn. The hazard index of the examined vegetables was found to increase in the following order: FMU > LMA > LVE > LCO > FAB > TXA > LAM > LXA > TMA, with values ranging from 1.84× <math> <msup><mrow><mn>10</mn></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </math> to 1.3. The hazard index for these vegetables indicated that only plantains had values over 1, suggesting potential non-cancer risks. The aggregate cancer risk associated with Cr, U, and Ni across each investigated sample increased in the following order: TMA > FMU > LMA > TXA > FAB > LVE > LXA > LCO > LAM, with values ranging from 1.04 × 10^-4 to 1.25 × 10^-3, with Ni the primary contributor. Overall, these findings suggest potential cancer risks associated with the consumption of these vegetables. It is therefore recommended that consumers, particularly those who frequently consume plantain from the investigated area, consider moderating their intake as a precautionary measure to mitigate potential health risks associated with chronic exposure to these metals through diet.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations of Metal Mixtures with Lipid Profiles: Exploring the Mediating Role of Testosterone in a Cross-Sectional Study.","authors":"Sencai Lin, Ying Yang, Junxiu He, Sihan Hu, Yu Bao, Xiaoting Ge, Xiaobo Yang","doi":"10.1007/s12011-025-04745-4","DOIUrl":"https://doi.org/10.1007/s12011-025-04745-4","url":null,"abstract":"<p><p>Metal exposure is a critical driver for dyslipidemia, yet the associations and underlying mechanisms between them remain uncertain. This study aimed to investigate the relationships between metal exposure, lipid profile, and total testosterone (TT) and to explore the mediating role of TT in 548 manganese-exposed male workers. We quantified 15 blood metals alongside serum lipid profiles [total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C)] and TT levels. Least absolute shrinkage and selection operator (LASSO) was used to select key metals. Bayesian kernel machine regression (BKMR) and generalized linear regression (GLM) were performed to explore the associations among key metals, lipid profiles and TT. Mediation analysis was used to evaluate mediating role of TT between metal and lipid profile. BKMR indicated that Pb-dominated (PIP = 0.72) mixtures (Pb, Cu, As, Se) elevated HDL-C, whereas Cu-dominated (PIP = 0.42) mixtures (Mg, Cu, As, Se) reduced TT. GLM revealed that Pb (β = 0.05) and Se (β = 0.18) were positively associated with HDL-C, whereas Cu was inversely correlated with TC (β =  - 0.16), HDL-C (β =  - 0.20) and LDL-C (β =  - 0.34). Mg (β =  - 0.31) and As (β = 0.09) were significantly associated with TT. Mediation analysis revealed that TT mediated 8% (P < 0.05) effect between Cu and LDL-C. In conclusion, exposure to metal was associated with lipid profile in male occupational population. TT may play a potential mediating role between Cu and LDL-C, emphasizing the potential mechanism of endocrine system in metal-induced lipid abnormalities.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimating the Global Burden and Health Risks Associated with Dietary and Cigarette-Related Cadmium Exposure.","authors":"Jianjiang Shao, Guanling Song, Yunhua Hu, Qiang Niu, Yizhong Yan","doi":"10.1007/s12011-025-04747-2","DOIUrl":"https://doi.org/10.1007/s12011-025-04747-2","url":null,"abstract":"<p><p>To quantitatively assess the global burden and health risks associated with dietary and cigarette-related cadmium (Cd) exposure, and to provide a scientific basis for developing public health policies for the prevention and control of global Cd exposure. This study combined the Cd concentration data in foods and cigarettes and their consumption data to assess the Cd exposure levels from these two sources. The toxicity kinetics (TK) model was used to convert dietary Cd exposure into urinary Cd (U-Cd) exposure to further evaluate the burden of cardiovascular disease (CVD) caused by dietary Cd exposure. The risk assessment model of the United States Environmental Protection Agency (USEPA) was adopted to evaluate the cancer and non-cancer risks caused by cigarette-related Cd exposure. The global average daily dietary Cd intake was 2.20 μg/kg bw/day. The daily Cd exposure to cigarettes was 0.0359 μg/kg bw/day. The proportions of the burden of CVD, stroke and coronary heart disease (CHD) explained by dietary Cd intake were 6.08%, 3.34% and 2.74%, respectively. The excess lifetime cancer risk (ELCR) due to cigarette-related Cd exposure was 0.0037, and the non-cancer hazard quotient (NCHQ) was 26.89. Cd exposure in diet and cigarettes significantly affects the global CVD burden and cancer risk. This study emphasizes the importance of reducing Cd exposure in the environment and formulating relevant policies to mitigate health risks, including strengthening Cd control in food and implementing effective and reasonable tobacco control policies.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Long-Term Fluoride Exposure: Systematic Review and Meta-Analysis on Anxiety and Depression in Animal Models.","authors":"Fernanda Marlen Enríquez-Sánchez, María Isabel Pérez-Vega, María de la Luz Miranda-Beltrán, Liliana Valdez-Jiménez","doi":"10.1007/s12011-025-04755-2","DOIUrl":"https://doi.org/10.1007/s12011-025-04755-2","url":null,"abstract":"<p><p>Studies have shown that long-term fluoride ingestion affects the structure and functioning of the central nervous system, altering cognitive functions of exposed individuals and mood disorders. The aim of this study is to present a systematic review and meta-analysis on the effects of experimental fluoride poisoning on anxiety and depression in animal models. The systematic review was performed according to PRISMA methodology, using the terms \"fluoride and depression\", \"fluoride and anxiety\" in PubMed, Scopus and International Society for Fluoride Research databases, on studies published between 2013-2025. Subsequently, a meta-analysis was conducted, and the risk of bias was evaluated using the SYRCLE methodology. The results demonstrated that prolonged fluoride exposure instigates substantial behavioral alterations in animal models, manifesting a pro-depressant effect in the tail suspension test (β = 1.53, p < 0.001) with an evident dose-response relationship (β = 0.069 per ppm, p = 0.0004). The analysis revealed a higher susceptibility in males (β = 2.68, p = 0.010) and rats exhibit different response patterns compared to mice (β = -1.72, p = 0.043). In anxiety tests, non-significant trends were observed in the elevated maze (β = -0.65, p = 0.108), but a significant reduction of rearing was observed in the open field (β = -0.85, p = 0.033). Despite considerable heterogeneity among the studies, the findings remained consistent in sensitivity analyses, indicating that fluoride exerts a differential effect on neural circuits implicated in emotional regulation. This observation carries significant ramifications for the assessment of neurobehavioral risks in exposed populations.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Egg Quality, Serum Parameters, and Bone Characteristics in Late-Phase Laying Hens Supplemented with Zinc, Manganese, and Copper with Sulfate or Amino Acids.","authors":"Arda Sözcü, Zehra Avcı Küpeli, Aydın İpek, Merve Gündüz","doi":"10.1007/s12011-025-04748-1","DOIUrl":"https://doi.org/10.1007/s12011-025-04748-1","url":null,"abstract":"<p><p>The aim of this study was to determine the effect of different zinc (Zn), manganese (Mn), and copper (Cu) combinations in different forms on egg quality, serum parameters, and bone characteristics in late-phase laying hens. A total of 600 Lohmann White layer hens at 64 weeks of age were divided into dietary groups with three repetitions (n = 40 hens/cage) in each group. The experimental groups were created as follows: control diet (Cont), supplementation of calcium carbonate (CaCO₃) as a mineral carrier (CaCO₃), sulfate form of Zn, Mn, and Cu (Sulf); Zn, Mn, and Cu-glycine chelate (Gly); Zn + Mn-methionine and Cu-lysine chelate (Met-Lys). The minerals were supplemented with an amount of 1 kg/ton of feed. The mean value of egg production was higher in both Gly (81.5%) and Met-Lys (81.4%) and the lowest in the Cont group (78.7%, P < 0.01). The eggshell thickness was found to be 0.397 mm in the Gly group and 0.399 mm in the Met-Lys group compared to the Cont (0.388 mm, P < 0.01). The lowest number of lymphocytes (34.6%) and the highest number of monocytes (26.3%) and basophils (7.0%) were observed in the Met-Lys group (P < 0.05). The plasma Zn was found to be the highest in the CaCO₃ group (176.2 µg/dL) and the lowest in the Cont (73.3 µg/dL) and Met-Lys groups (66.3 µg/dL, P < 0.001). The breaking strength and the ash content of the tibia and femur were found to be the highest in the Met-Lys group (P < 0.05). The Met-Lys supplementation could improve egg production, egg weight, and eggshell thickness, provide a hepatoprotective effects, and strengthen the effect for femur and tibia bones in laying hens during the late term of the laying period.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}